CN108378018A - A kind of specimen preserving liquid and its preparation method and application - Google Patents
A kind of specimen preserving liquid and its preparation method and application Download PDFInfo
- Publication number
- CN108378018A CN108378018A CN201810307637.3A CN201810307637A CN108378018A CN 108378018 A CN108378018 A CN 108378018A CN 201810307637 A CN201810307637 A CN 201810307637A CN 108378018 A CN108378018 A CN 108378018A
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- China
- Prior art keywords
- parts
- preserving liquid
- specimen preserving
- realgar
- isothiazolone
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of specimen preserving liquids, are mixed by weight ratio by following raw material：10 20 parts of methanol, 13 parts of polyoxyethylene aliphatic alcohol ether, 8 12 parts of hexanol, 24 parts of benzalkonium bromide, 57 parts of salt, 57 parts of phenol, 48 parts of potassium nitrate, 10 20 parts of alum, 5 15 parts of glycerine, 35 parts of benzoic acid, 68 parts of isothiazolone, 57 parts of picric acid, 35 parts of realgar.The present invention preserves liquid and is free of formaldehyde, and volatilization is slow, has no irritating odor, raw material is easy to get, of low cost, safe, preservation effect is good, long period preservation will not go mouldy, enable to sample water suction moisturizing, be not easy it is dry, without precipitation, color is natural, do not shrink, remains flexible state, can repeatedly use, it should not damage, and poisonous and harmful substance is free of, it is environmentally protective to the infringement of human body to eliminate formaldehyde, mitigates the burden of environment.
Description
Technical field
The present invention relates to Saving specimen technical field, specifically a kind of specimen preserving liquid and its preparation method and application.
Background technology
Formalin specimen preserving liquid has good fixed function, this is because the functional group of formaldehyde and sample protein
A large amount of to combine, long-term placement further polymerization forms paraformaldehyde.This process is allowed to form in protein peptide interchain suitable
The tissue morphology of sample can be sterilized and be kept to the crosslinking of degree.But formaldehyde, benzene class are determined as by the World Health Organization
Carcinogen is listed in high poison chemicals, meeting intense stimulus eyes, skin, respiratory mucosa etc., it is different to ultimately cause immune function
Often, hepatic injury and central nervous system are affected, will cause fetal anomaly, leukaemia, chronic respiratory disease, nasopharyngeal carcinoma,
The diseases such as feminine menstrual disorder, acute confusion depression, serious threat is caused to health.Simultaneously because formalin
Low boiling point, therefore the highly volatile in room temperature are accelerated with the rising evaporation rate of temperature, give out strong impulse smell,
Serious pollution laboratory environment.Over time, air formaldehyde concentration also constantly increases, and to medicine, biology and moves
The health of object worker, which causes, to be seriously threatened.In addition, the sample through formalin long-term preservation, it may occur that go mouldy, xanthochromia,
Phenomena such as sample is hardened.
Invention content：
The object of the present invention is to provide a kind of specimen preserving liquids and its preparation method and application.The preservation liquid is free of first
Aldehyde, volatilization is slow, has no irritating odor, toxicity is very low, safe, will not pollute experimental situation, and preservation effect is good, the long period
Preservation will not go mouldy, sample soft texture, and color and luster is good.
In order to solve the above technical problems, the present invention is realized using following technical scheme：
A kind of specimen preserving liquid is mixed by weight ratio by following raw material：
10-20 parts of methanol, 1-3 parts of polyoxyethylene aliphatic alcohol ether, 8-12 parts of hexanol, 2-4 parts of benzalkonium bromide, 5-7 parts of salt, phenol
5-7 parts, 4-8 parts of potassium nitrate, 10-20 parts of alum, 5-15 parts of glycerine, 3-5 parts of benzoic acid, 6-8 parts of isothiazolone, picric acid 5-7
Part, 3-5 parts of realgar.
Further, a kind of specimen preserving liquid is mixed by weight ratio by following raw material：
10 parts of methanol, 1 part of polyoxyethylene aliphatic alcohol ether, 8 parts of hexanol, 2 parts of benzalkonium bromide, 5 parts of salt, 5 parts of phenol, potassium nitrate 4
Part, 10 parts of alum, 5 parts of glycerine, 3 parts of benzoic acid, 6 parts of isothiazolone, 5 parts of picric acid, 3 parts of realgar.
Further, a kind of specimen preserving liquid is mixed by weight ratio by following raw material：
15 parts of methanol, 2 parts of polyoxyethylene aliphatic alcohol ether, 10 parts of hexanol, 3 parts of benzalkonium bromide, 6 parts of salt, 6 parts of phenol, potassium nitrate 6
Part, 15 parts of alum, 10 parts of glycerine, 4 parts of benzoic acid, 7 parts of isothiazolone, 6 parts of picric acid, 4 parts of realgar.
Further, a kind of specimen preserving liquid is mixed by weight ratio by following raw material：
20 parts of methanol, 3 parts of polyoxyethylene aliphatic alcohol ether, 12 parts of hexanol, 4 parts of benzalkonium bromide, 7 parts of salt, 7 parts of phenol, potassium nitrate 8
Part, 20 parts of alum, 15 parts of glycerine, 5 parts of benzoic acid, 8 parts of isothiazolone, 7 parts of picric acid, 5 parts of realgar.
The preparation method of above-mentioned specimen preserving liquid, includes the following steps：
（1）By polyoxyethylene aliphatic alcohol ether, benzalkonium bromide, salt, phenol, potassium nitrate, alum, benzoic acid, isothiazolone, bitter taste
Acid, realgar are put into methanol and stir evenly, spare；
（2）In step（1）Hexanol is added after being completely dissolved in the product, glycerine stirs evenly to get specimen preserving liquid.
Step（1）The mixing speed is 200-400r/min.
Step（2）The mixing speed is 300-500r/min.
Step（2）The mixing time is 1-3h.
The application method of above-mentioned specimen preserving liquid, includes the following steps：Total body perfusion first is carried out to sample with fixer, then
Liquid, which is preserved, with the present invention carries out immersion preservation, you can.
The fixer is not particularly limited, using conventional commercial product.
Compared with the prior art, the advantages of the present invention are as follows：The present invention preserves liquid and is free of formaldehyde, and volatilization is slow, nonirritant
Smell, raw material are easy to get, of low cost, safe, and preservation effect is good, and long period preservation will not go mouldy, and enable to
Sample water suction moisturizing, be not easy it is dry, without precipitation, color is natural, do not shrink, remains flexible state, can repeatedly use,
It should not damage, and be free of poisonous and harmful substance, it is environmentally protective to the infringement of human body to eliminate formaldehyde, mitigates the burden of environment.
Specific implementation mode
The content for using specific embodiment to further illustrate the present invention below.
Embodiment 1
A kind of specimen preserving liquid is mixed by weight ratio by following raw material：
10 parts of methanol, 1 part of polyoxyethylene aliphatic alcohol ether, 8 parts of hexanol, 2 parts of benzalkonium bromide, 5 parts of salt, 5 parts of phenol, potassium nitrate 4
Part, 10 parts of alum, 5 parts of glycerine, 3 parts of benzoic acid, 6 parts of isothiazolone, 5 parts of picric acid, 3 parts of realgar.
The preparation method of above-mentioned specimen preserving liquid, includes the following steps：
（1）By polyoxyethylene aliphatic alcohol ether, benzalkonium bromide, salt, phenol, potassium nitrate, alum, benzoic acid, isothiazolone, bitter taste
Acid, realgar are put into methanol and stir evenly, spare；
（2）In step（1）Hexanol is added after being completely dissolved in the product, glycerine stirs evenly to get specimen preserving liquid.
Step（1）The mixing speed is 200r/min.
Step（2）The mixing speed is 300r/min.
Step（2）The mixing time is 1h.
The application method of above-mentioned specimen preserving liquid, includes the following steps：Total body perfusion first is carried out to sample with fixer, then
Liquid, which is preserved, with the present invention carries out immersion preservation, you can.The fixer is not particularly limited, and is using conventional commercial product
It can.
Embodiment 2
A kind of specimen preserving liquid is mixed by weight ratio by following raw material：
15 parts of methanol, 2 parts of polyoxyethylene aliphatic alcohol ether, 10 parts of hexanol, 3 parts of benzalkonium bromide, 6 parts of salt, 6 parts of phenol, potassium nitrate 6
Part, 15 parts of alum, 10 parts of glycerine, 4 parts of benzoic acid, 7 parts of isothiazolone, 6 parts of picric acid, 4 parts of realgar.
The preparation method of above-mentioned specimen preserving liquid, includes the following steps：
（1）By polyoxyethylene aliphatic alcohol ether, benzalkonium bromide, salt, phenol, potassium nitrate, alum, benzoic acid, isothiazolone, bitter taste
Acid, realgar are put into methanol and stir evenly, spare；
（2）In step（1）Hexanol is added after being completely dissolved in the product, glycerine stirs evenly to get specimen preserving liquid.
Step（1）The mixing speed is 300r/min.
Step（2）The mixing speed is 400r/min.
Step（2）The mixing time is 2h.
The application method of above-mentioned specimen preserving liquid, includes the following steps：Total body perfusion first is carried out to sample with fixer, then
Liquid, which is preserved, with the present invention carries out immersion preservation, you can.The fixer is not particularly limited, and is using conventional commercial product
It can.
Embodiment 3
A kind of specimen preserving liquid is mixed by weight ratio by following raw material：
20 parts of methanol, 3 parts of polyoxyethylene aliphatic alcohol ether, 12 parts of hexanol, 4 parts of benzalkonium bromide, 7 parts of salt, 7 parts of phenol, potassium nitrate 8
Part, 20 parts of alum, 15 parts of glycerine, 5 parts of benzoic acid, 8 parts of isothiazolone, 7 parts of picric acid, 5 parts of realgar.
The preparation method of above-mentioned specimen preserving liquid, includes the following steps：
（1）By polyoxyethylene aliphatic alcohol ether, benzalkonium bromide, salt, phenol, potassium nitrate, alum, benzoic acid, isothiazolone, bitter taste
Acid, realgar are put into methanol and stir evenly, spare；
（2）In step（1）Hexanol is added after being completely dissolved in the product, glycerine stirs evenly to get specimen preserving liquid.
Step（1）The mixing speed is 400r/min.
Step（2）The mixing speed is 500r/min.
Step（2）The mixing time is 3h.
The application method of above-mentioned specimen preserving liquid, includes the following steps：Total body perfusion first is carried out to sample with fixer, then
Liquid, which is preserved, with the present invention carries out immersion preservation, you can.The fixer is not particularly limited, and is using conventional commercial product
It can.
The above content is a further detailed description of the present invention in conjunction with specific preferred embodiments, and it cannot be said that
The specific implementation of the present invention is confined to these explanations.For those of ordinary skill in the art to which the present invention belongs, exist
Under the premise of not departing from present inventive concept, a number of simple deductions or replacements can also be made, all shall be regarded as belonging to the present invention's
Protection domain.
Claims (8)
1. a kind of specimen preserving liquid, it is characterized in that：It is mixed by weight ratio by following raw material：
10-20 parts of methanol, 1-3 parts of polyoxyethylene aliphatic alcohol ether, 8-12 parts of hexanol, 2-4 parts of benzalkonium bromide, 5-7 parts of salt, phenol
5-7 parts, 4-8 parts of potassium nitrate, 10-20 parts of alum, 5-15 parts of glycerine, 3-5 parts of benzoic acid, 6-8 parts of isothiazolone, picric acid 5-7
Part, 3-5 parts of realgar.
2. specimen preserving liquid according to claim 1, it is characterized in that：It is mixed by weight ratio by following raw material：
10 parts of methanol, 1 part of polyoxyethylene aliphatic alcohol ether, 8 parts of hexanol, 2 parts of benzalkonium bromide, 5 parts of salt, 5 parts of phenol, potassium nitrate 4
Part, 10 parts of alum, 5 parts of glycerine, 3 parts of benzoic acid, 6 parts of isothiazolone, 5 parts of picric acid, 3 parts of realgar.
3. specimen preserving liquid according to claim 1, it is characterized in that：It is mixed by weight ratio by following raw material：
15 parts of methanol, 2 parts of polyoxyethylene aliphatic alcohol ether, 10 parts of hexanol, 3 parts of benzalkonium bromide, 6 parts of salt, 6 parts of phenol, potassium nitrate 6
Part, 15 parts of alum, 10 parts of glycerine, 4 parts of benzoic acid, 7 parts of isothiazolone, 6 parts of picric acid, 4 parts of realgar.
4. specimen preserving liquid according to claim 1, it is characterized in that：It is mixed by weight ratio by following raw material：
20 parts of methanol, 3 parts of polyoxyethylene aliphatic alcohol ether, 12 parts of hexanol, 4 parts of benzalkonium bromide, 7 parts of salt, 7 parts of phenol, potassium nitrate 8
Part, 20 parts of alum, 15 parts of glycerine, 5 parts of benzoic acid, 8 parts of isothiazolone, 7 parts of picric acid, 5 parts of realgar.
5. a kind of preparation method of specimen preserving liquid, it is characterized in that：Include the following steps：
（1）By polyoxyethylene aliphatic alcohol ether, benzalkonium bromide, salt, phenol, potassium nitrate, alum, benzoic acid, isothiazolone, bitter taste
Acid, realgar are put into methanol and stir evenly, spare；
（2）In step（1）Hexanol is added after being completely dissolved in the product, glycerine stirs evenly to get specimen preserving liquid.
6. the preparation method of specimen preserving liquid according to claim 5, it is characterized in that：Step（1）The mixing speed is
200-400r/min。
7. the preparation method of specimen preserving liquid according to claim 5, it is characterized in that：Step（2）The mixing speed is
300-500r/min。
8. the preparation method of specimen preserving liquid according to claim 5, it is characterized in that：Step（2）The mixing time is
1-3h。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810307637.3A CN108378018A (en) | 2018-04-08 | 2018-04-08 | A kind of specimen preserving liquid and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810307637.3A CN108378018A (en) | 2018-04-08 | 2018-04-08 | A kind of specimen preserving liquid and its preparation method and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108378018A true CN108378018A (en) | 2018-08-10 |
Family
ID=63072657
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810307637.3A Withdrawn CN108378018A (en) | 2018-04-08 | 2018-04-08 | A kind of specimen preserving liquid and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108378018A (en) |
-
2018
- 2018-04-08 CN CN201810307637.3A patent/CN108378018A/en not_active Withdrawn
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Application publication date: 20180810 |