CN108354059A - A kind of production method of cottonseed protein - Google Patents
A kind of production method of cottonseed protein Download PDFInfo
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- CN108354059A CN108354059A CN201711472292.9A CN201711472292A CN108354059A CN 108354059 A CN108354059 A CN 108354059A CN 201711472292 A CN201711472292 A CN 201711472292A CN 108354059 A CN108354059 A CN 108354059A
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- Prior art keywords
- cottonseed protein
- dephenolize
- production method
- cottonseed
- degreasing
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- 235000012343 cottonseed oil Nutrition 0.000 title claims abstract description 86
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 70
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 70
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 37
- 229920000742 Cotton Polymers 0.000 claims abstract description 63
- 210000001161 mammalian embryo Anatomy 0.000 claims abstract description 53
- 108090000790 Enzymes Proteins 0.000 claims abstract description 44
- 102000004190 Enzymes Human genes 0.000 claims abstract description 44
- 238000001035 drying Methods 0.000 claims abstract description 41
- 238000005238 degreasing Methods 0.000 claims abstract description 32
- 238000005554 pickling Methods 0.000 claims abstract description 25
- 238000001556 precipitation Methods 0.000 claims abstract description 12
- 239000000835 fiber Substances 0.000 claims abstract description 8
- 238000005096 rolling process Methods 0.000 claims abstract description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 69
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 46
- 229940088598 enzyme Drugs 0.000 claims description 43
- 235000019441 ethanol Nutrition 0.000 claims description 24
- 239000000463 material Substances 0.000 claims description 14
- 229920005610 lignin Polymers 0.000 claims description 13
- 108010059892 Cellulase Proteins 0.000 claims description 10
- 229940106157 cellulase Drugs 0.000 claims description 10
- 230000003647 oxidation Effects 0.000 claims description 10
- 238000007254 oxidation reaction Methods 0.000 claims description 10
- 239000002253 acid Substances 0.000 claims description 8
- 238000005266 casting Methods 0.000 claims description 8
- 239000012535 impurity Substances 0.000 claims description 7
- 238000012546 transfer Methods 0.000 claims description 7
- 230000008030 elimination Effects 0.000 claims description 4
- 238000003379 elimination reaction Methods 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 2
- 230000001590 oxidative effect Effects 0.000 claims description 2
- 238000007873 sieving Methods 0.000 claims description 2
- QBKSWRVVCFFDOT-UHFFFAOYSA-N gossypol Chemical compound CC(C)C1=C(O)C(O)=C(C=O)C2=C(O)C(C=3C(O)=C4C(C=O)=C(O)C(O)=C(C4=CC=3C)C(C)C)=C(C)C=C21 QBKSWRVVCFFDOT-UHFFFAOYSA-N 0.000 abstract description 24
- 235000019198 oils Nutrition 0.000 abstract description 18
- QHOPXUFELLHKAS-UHFFFAOYSA-N Thespesin Natural products CC(C)c1c(O)c(O)c2C(O)Oc3c(c(C)cc1c23)-c1c2OC(O)c3c(O)c(O)c(C(C)C)c(cc1C)c23 QHOPXUFELLHKAS-UHFFFAOYSA-N 0.000 abstract description 12
- 229930000755 gossypol Natural products 0.000 abstract description 12
- 229950005277 gossypol Drugs 0.000 abstract description 12
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 abstract description 10
- 238000005516 engineering process Methods 0.000 abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 abstract description 3
- 229940072033 potash Drugs 0.000 abstract description 3
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 abstract description 3
- 235000015320 potassium carbonate Nutrition 0.000 abstract description 3
- 238000005457 optimization Methods 0.000 abstract description 2
- 238000012545 processing Methods 0.000 abstract description 2
- 241000219146 Gossypium Species 0.000 description 57
- 235000018102 proteins Nutrition 0.000 description 55
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 18
- 238000002474 experimental method Methods 0.000 description 11
- 238000000034 method Methods 0.000 description 10
- 238000012216 screening Methods 0.000 description 10
- 235000012054 meals Nutrition 0.000 description 5
- 230000000694 effects Effects 0.000 description 3
- 230000001007 puffing effect Effects 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 2
- 239000005864 Sulphur Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- WQYVRQLZKVEZGA-UHFFFAOYSA-N hypochlorite Chemical compound Cl[O-] WQYVRQLZKVEZGA-UHFFFAOYSA-N 0.000 description 2
- 238000000465 moulding Methods 0.000 description 2
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 240000000047 Gossypium barbadense Species 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 108010064851 Plant Proteins Proteins 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000003818 cinder Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 235000014103 egg white Nutrition 0.000 description 1
- 210000000969 egg white Anatomy 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 235000021118 plant-derived protein Nutrition 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
- A23J1/148—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds by treatment involving enzymes or microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention discloses a kind of production methods of cottonseed protein, belong to cottonseed processing field, including clean, peel off, being crushed, rolling embryo, degreasing dephenolize, precipitation, drying process, further include that hair light seed is carried out pickling, complex enzyme is added in degreasing dephenolize.The improvement that the present invention passes through optimization and equipment to production technology, improve the purity and potash hydroxide solubility of cottonseed protein, the content for reducing cotton oil, gossypol, crude fibre and coarse ash, to improve the quality of cottonseed protein with its as feed value, widened the purposes range of cottonseed protein.
Description
Technical field
The present invention relates to the separation and Extraction of natural plant protein, especially a kind of production method of cottonseed protein belongs to cotton
Seed manufacture field.
Background technology
Cottonseed protein be by cottonseed stripping cotton lint, peel off, it is broken after extract a kind of protein-rich product prepared.China is one
More than 1,000 ten thousand tons of cottonseed is often produced in the country that protein resource lacks, the whole nation per year, and cottonseed protein can be used as a kind of protein resource and be used for
In animal feed, therefore, produce high-quality cottonseed protein can make up to a certain extent protein resource deficiency and
There is huge economic benefit.
There are many production method of cottonseed protein, mainly there is hot moulding production and puffing production.It is prepared by hot moulding and puffing production
Cheng Jun uses high-temperature process, and high-temperature process can make cottonseed protein be destroyed and be denaturalized to a certain extent, causes cottonseed egg
White purity is deteriorated, potash hydroxide solubility reduces；In addition, puffing production is higher to equipment requirement, process conditions require harshness,
Condition control is bad easily to cause expansion effect poor, and cottonseed protein becomes sticky and influences follow-up detoxification program.
Influence in order to avoid high-temperature process to cottonseed protein production process, improved processing step are to clean, peel off, breaking
It is broken, roll embryo, infuser degreasing dephenolize, precipitation, drying, entire technical process carries out at low temperature, avoids high-temperature process to cotton
The damage of seed albumen；But the content of the cottonseed protein crude fibre and coarse ash gone out using this improved technique productions occupies height not
Under；Secondly, infuser is using traditional tow chain type leacher or loop type extractor, no matter which kind of infuser, so that material exists
It is accumulated in infuser, degreasing dephenolize process materials cannot come into full contact with solution, cause degreasing dephenolize insufficient, these are all final
Reduce the quality of cottonseed protein；In addition, clasmatosis is insufficient, cause the releases such as cottonseed protein, gossypol, cotton oil incomplete,
Degreasing, insufficient dephenolize are also a key factor for influencing cottonseed protein quality.
Invention content
The technical problem to be solved in the invention is to provide a kind of production method of cottonseed protein, can reduce cottonseed protein
The content of middle crude fibre, coarse ash, cotton oil, gossypol etc. promotes the quality of cottonseed protein and its value as feed, widens cotton
The purposes range of seed albumen.
In order to solve the above technical problems, the technical solution adopted in the present invention is：
A kind of production method of cottonseed protein includes cleaning, peeling off, being crushed, rolling embryo, degreasing dephenolize, precipitation, drying process：
Further include that hair light seed is subjected to pickling；Complex enzyme is added in degreasing dephenolize.
Technical solution of the present invention further improvement lies in that including the following steps：
A. gross cottonseed cleaned, shell suede, obtain a mao light seed；
B. hair light seed is subjected to pickling, obtains light seed；
C. light seed peeled off, be crushed, then impurity elimination, obtain cotton benevolence；
D. cotton benevolence is carried out obtaining embryo piece after rolling embryo, then drying, degreasing dephenolize, obtain the wet cotton dregs of dephenolize；
E. the wet cotton dregs of dephenolize are crushed after drying, precipitation, are sieved, and the fines sifted out is sorted again, obtains cottonseed
Albumen.
Technical solution of the present invention further improvement lies in that：The complex enzyme includes cellulase and lignin oxidation's enzyme,
The mass ratio of complex enzyme and the embryo piece after drying is 1g:The mass ratio of 5~10kg, cellulase and lignin oxidation's enzyme is 1:1
~1.2.
Technical solution of the present invention further improvement lies in that：The acid solution used when pickling is sulfuric acid solution, sulfuric acid solution
The mass volume ratio of a concentration of 0.12~0.13mol/L, hair light seed and sulfuric acid solution is 100g:0.15~0.2L, pickling when
Between be 10~15min.
Technical solution of the present invention further improvement lies in that：Using sieve is thrown when sieving impurity elimination, the material casting speed for throwing sieve is 18
~21m/s.
Technical solution of the present invention further improvement lies in that：It rolls proembryo first to soften cotton benevolence in softening kettle, soften
Temperature is 82~88 DEG C afterwards；The thickness of the embryo piece is 0.2~0.3mm.
Technical solution of the present invention further improvement lies in that：The dephenolize is handled using ethanol solution, ethanol solution
A concentration of 50~55%, the mass volume ratio of embryo piece and ethanol solution after drying is 1t:0.4~0.7m3。
Technical solution of the present invention further improvement lies in that：Drying is dried at 65~75 DEG C with plate-press dryer
Dry, degreasing, dephenolize are carried out at the same time using the tow chain type leacher of strip wing, and temperature when degreasing, dephenolize is 40~55 DEG C.
Technical solution of the present invention further improvement lies in that：Sorting is carried out using sieve is turned, and turns sieve transfer sieve plate
Aperture is 16~20 mesh, and the rotating speed of rotating cylinder is 30~60r/min.
By adopting the above-described technical solution, the technological progress that the present invention obtains is：
The present invention improves the purity and potassium hydroxide of cottonseed protein by the improvement of optimization and equipment to production technology
Solubility, reduces the content of cotton oil, gossypol, crude fibre and coarse ash, and then improves the feed value of cottonseed protein, widens
The purposes range of cottonseed protein.
Hair light seed is carried out pickling by the present invention, reduces the content of crude fibre in cottonseed protein, coarse ash.Pickling of the present invention
Acid used is preferably sulfuric acid solution, a concentration of 0.12~0.13mol/L of sulfuric acid solution, the mass body of hair light seed and sulfuric acid solution
Product is than being 100g:0.15~0.2L, within the scope of this optimum condition, sulfuric acid solution can degrade the binding site of short flannel and boll hull,
The remaining short flannel of hair light seed surface can be from being detached from boll hull, being dissolved into sulfuric acid solution, to reduce by short after 10~15min
Suede takes the coarse-fibred content in cottonseed protein to；In addition, the inorganic salts such as the un-cleared sandstone of stripping suede step that clean before pickling
Class is also dissolved into sulfuric acid solution in acid cleaning process and is removed, these all reduce the content of coarse ash in cottonseed protein,
Improve the quality of cottonseed protein.
The present invention peels off light seed, it is broken after, successively pass through angle sieve, rotary flat sieve and throw and sieved, obtained
Cotton benevolence impurity it is less, further improve the purity of cottonseed protein.Light seed is peeled off, it is broken after, cotton benevolence and cotton seed hulls etc. are miscellaneous
Matter is mixed in together, successively passes through tertiary screen, and under the best material casting speed for throwing 18~21m/s of sieve, can be by cotton seed hulls etc.
Other impurities fully remove.
The selection of the thickness and infuser of embryo piece of the present invention, improves the dynamics of degreasing dephenolize, reduces in cottonseed protein
The content of cotton oil, gossypol.The thickness for the embryo piece that flaker is shut out is 0.2~0.3mm, and embryo piece is relatively thin, embryo piece and degreasing, dephenolize institute
It is larger with the contact area of solvent；Infuser is preferably the tow chain type leacher of strip wing, in the base of traditional tow chain type leacher
Material wing is increased on plinth, if can material be divided into stem portion, so as to avoid accumulation of the material in infuser, also increases object
Material and degreasing, the contact area of dephenolize solvent for use, these are all conducive to the abjection of cotton oil, gossypol, ensure that degreasing dephenolize is anti-
Answer smooth simultaneously fully carries out.
Preferably by ethanol solution when dephenolize of the present invention, ethanol solution is compared with methanol solution used in traditional handicraft, gossypol
Solubility in ethanol solution is larger so that gossypol can be fully removed, and ethyl alcohol is weaker than the toxicity of methanol very much, one
Determine to improve the safety of product in degree, reduce operational danger；Secondly as ethyl alcohol has with 6# oil used when degreasing
Stronger compatibility, this enables degreasing and dephenolize to be carried out at the same time, simplifies production technology, shortens the reaction time, improves
Production efficiency；Again, it is added complex enzyme when degreasing, dephenolize, the mixed enzyme of complex enzyme preferred lignin oxidation's enzyme and cellulase,
The mass ratio of cellulase and lignin oxidation's enzyme is 1:1~1.2, complex enzyme with drying after embryo piece mass ratio be 1g:5~
10kg, within the scope of this optimum condition, cellulose and lignin in decomposable asymmetric choice net embryo piece are conducive to the decomposition of embryo piece medium staple, shell,
Improve the content of cottonseed protein；In addition, when degreasing, dephenolize temperature be 40~55 DEG C, in this temperature range, complex enzyme activity compared with
Height, lignin oxidation's enzyme and cellulase can mutually promote, and play the role of better lignin degrading and cellulose.
The entire production technology of the present invention carries out under cryogenic, and effectively cottonseed protein can be avoided in high temperature
Lower degradation, denaturation ensure that the activity of cottonseed protein and chemical constitution are not destroyed.Temperature is 82~88 after cotton embryo softening of the present invention
DEG C, temperature when drying embryo piece is 65~75 DEG C, and temperature is relatively low, will not destroy the stability of cottonseed protein or occur secondary anti-
It answers, to ensure that high-dissolvability and purity of the cottonseed protein in potassium hydroxide solution.
The present invention first dries the wet cotton dregs obtained after degreasing dephenolize, then carries out precipitation, crushing and sieve, and turns the web plate of sieve
Aperture aperture is 16~20 mesh, and the rotating speed of rotating cylinder is that 30~60r/min can be by boll hull, bobbles within the scope of this optimum condition
Equal impurity screen out completely, further ensure the high-purity of cottonseed protein.
Specific implementation mode
The present invention is described in further details with reference to embodiment：
A kind of production method of cottonseed protein, includes the following steps：
A. gross cottonseed is cleaned using wind-force, screening trommel, shells suede using twice delinter, obtains a mao light seed；
B. hair light seed is subjected to pickling, acid pickling liquid is oxidizing acid, such as sulfuric acid solution, hypochlorite solution or nitric acid
Solution, preferably sulfuric acid solution, a concentration of 0.12~0.13mol/L of sulfuric acid solution used, the matter of hair light seed and sulfuric acid solution
Amount volume ratio is 100g:The time of 0.15~0.2L, pickling are 10~15min, and light seed is obtained after pickling；
C. light seed peeled off, be crushed, then sieved through angle sieve, rotary flat sieve, throwing, wherein throwing the material casting speed of sieve
Degree is 18~21m/s, obtains cotton benevolence；
D. cotton benevolence is moved into softening kettle and is softened, the temperature of cotton benevolence is 82~88 DEG C after softening；It again will with flaker
Cotton benevolence rolls embryo, and obtained embryo piece thickness is 0.2~0.3mm, and embryo piece is then carried out low temperature drying with plate-press dryer, when drying
Temperature be 65~75 DEG C；Embryo piece after drying moves into the tow chain type leacher of strip wing, and quantitative 6# oil, ethanol solution is added
With complex enzyme carry out degreasing, dephenolize, a concentration of the 50~55% of ethanol solution used, the quality of embryo piece and ethanol solution after drying
Volume ratio is 1t:0.4~0.7m3；Complex enzyme is cellulase and lignin oxidation's enzyme is 1 in mass ratio:1~1.2 is mixed
The mass ratio of the enzyme of conjunction, complex enzyme and the embryo piece after drying is 1g:5~10kg；
E. the wet cotton dregs of dephenolize are obtained after dephenolize, wet cotton dregs are first dried, then carry out precipitation, then crush,
Screening, the fines sifted out, which enters to turn to sieve, to be sorted, and the aperture for turning sieve transfer sieve plate is 16~20 mesh, and the rotating speed of rotating cylinder is 30
Boll hull, bobbles and Cottonseed Meal are screened out, obtain cottonseed protein by~60r/min, and cottonseed protein is measured and packed.
Protein content >=65% in the cottonseed protein that this production method is finally produced, cotton oil≤0.4%, gossypol≤
0.02%, crude fibre≤1%, coarse ash≤2%.
Embodiment 1
A kind of production method of cottonseed protein, includes the following steps：
A. gross cottonseed is cleaned using wind-force, screening trommel, shells suede using twice delinter, obtains a mao light seed；
B. hair light seed is subjected to pickling, a concentration of 0.13mol/L of sulfuric acid solution used, hair light seed and sulphur with sulfuric acid solution
The mass volume ratio of acid solution is 100g:The time of 0.15L, pickling are 10min, and light seed is obtained after pickling；
C. light seed peeled off, be crushed, then sieved through angle sieve, rotary flat sieve, throwing, wherein throwing the material casting speed of sieve
Degree is 20m/s, obtains cotton benevolence；
D. cotton benevolence is moved into softening kettle and is softened, the temperature of cotton benevolence is 82 DEG C after softening；Again with flaker by cotton benevolence
Embryo is rolled, obtained embryo piece thickness is 0.2mm, and embryo piece is then carried out low temperature drying with plate-press dryer, and temperature when drying is
65℃；Embryo piece after drying moves into the tow chain type leacher of strip wing, and quantitative 6# oil, ethanol solution and complex enzyme is added and carries out
Degreasing, dephenolize, a concentration of the 55% of ethanol solution used, the mass volume ratio of embryo piece and ethanol solution is 1t after drying:
0.4m3；Complex enzyme is cellulase and lignin oxidation's enzyme is 1 in mass ratio:After 1 enzyme mixed, complex enzyme and drying
Embryo piece mass ratio be 1g:7kg；
E. the wet cotton dregs of dephenolize are obtained after dephenolize, wet cotton dregs are first dried, then carry out precipitation, then crush,
Screening, the fines sifted out, which enters to turn to sieve, to be sorted, and the aperture for turning sieve transfer sieve plate is 16 mesh, and the rotating speed of rotating cylinder is 45r/
Boll hull, bobbles and Cottonseed Meal are screened out, obtain cottonseed protein by min, and cottonseed protein is measured and packed.
Embodiment 2
A kind of production method of cottonseed protein, includes the following steps：
A. gross cottonseed is cleaned using wind-force, screening trommel, shells suede using twice delinter, obtains a mao light seed；
B. hair light seed is subjected to pickling with sulfuric acid solution, a concentration of 0.125mol/L of sulfuric acid solution used, hair light seed and
The mass volume ratio of sulfuric acid solution is 100g:The time of 0.18L, pickling are 12min, and light seed is obtained after pickling；
C. light seed peeled off, be crushed, then sieved through angle sieve, rotary flat sieve, throwing, wherein throwing the material casting speed of sieve
Degree is 18m/s, obtains cotton benevolence；
D. cotton benevolence is moved into softening kettle and is softened, the temperature of cotton benevolence is 85 DEG C after softening；Again with flaker by cotton benevolence
Embryo is rolled, obtained embryo piece thickness is 0.3mm, and embryo piece is then carried out low temperature drying with plate-press dryer, and temperature when drying is
70℃；Embryo piece after drying moves into the tow chain type leacher of strip wing, and quantitative 6# oil, ethanol solution and complex enzyme is added and carries out
Degreasing, dephenolize, a concentration of the 53% of ethanol solution used, the mass volume ratio of embryo piece and ethanol solution is 1t after drying:
0.7m3；Complex enzyme is cellulase and lignin oxidation's enzyme is 1 in mass ratio:1.2 enzymes mixed, complex enzyme and drying
The mass ratio of embryo piece afterwards is 1g:5kg；
E. the wet cotton dregs of dephenolize are obtained after dephenolize, wet cotton dregs are first dried, then carry out precipitation, then crush,
Screening, the fines sifted out, which enters to turn to sieve, to be sorted, and the aperture for turning sieve transfer sieve plate is 18 mesh, and the rotating speed of rotating cylinder is 30r/
Boll hull, bobbles and Cottonseed Meal are screened out, obtain cottonseed protein by min, and cottonseed protein is measured and packed.
Embodiment 3
A kind of production method of cottonseed protein, includes the following steps：
A. gross cottonseed is cleaned using wind-force, screening trommel, shells suede using twice delinter, obtains a mao light seed；
B. hair light seed is subjected to pickling, a concentration of 0.12mol/L of sulfuric acid solution used, hair light seed and sulphur with sulfuric acid solution
The mass volume ratio of acid solution is 100g:The time of 0.2L, pickling are 15min, and light seed is obtained after pickling；
C. light seed peeled off, be crushed, then sieved through angle sieve, rotary flat sieve, throwing, wherein throwing the material casting speed of sieve
Degree is 21m/s, obtains cotton benevolence；
D. cotton benevolence is moved into softening kettle and is softened, the temperature of cotton benevolence is 88 DEG C after softening；Again with flaker by cotton benevolence
Embryo is rolled, obtained embryo piece thickness is 0.25mm, and embryo piece is then carried out low temperature drying with plate-press dryer, and temperature when drying is
75℃；Embryo piece after drying moves into the tow chain type leacher of strip wing, and quantitative 6# oil, ethanol solution and complex enzyme is added and carries out
Degreasing, dephenolize, a concentration of the 50% of ethanol solution used, the mass volume ratio of embryo piece and ethanol solution is 1t after drying:
0.6m3；Complex enzyme is cellulase and lignin oxidation's enzyme is 1 in mass ratio:1.1 enzymes mixed, complex enzyme and drying
The mass ratio of embryo piece afterwards is 1g:10kg；
E. the wet cotton dregs of dephenolize are obtained after dephenolize, wet cotton dregs are first dried, then carry out precipitation, then crush,
Screening, the fines sifted out, which enters to turn to sieve, to be sorted, and the aperture for turning sieve transfer sieve plate is 20 mesh, and the rotating speed of rotating cylinder is 60r/
Boll hull, bobbles and Cottonseed Meal are screened out, obtain cottonseed protein by min, and cottonseed protein is measured and packed.
Check experiment
A kind of production method of cottonseed protein, includes the following steps：
A. gross cottonseed is cleaned using wind-force, screening trommel, shells suede using twice delinter, obtains a mao light seed；
B. hair light seed peeled off, be crushed, then sieved through angle sieve, rotary flat sieve, throwing, wherein throwing the material casting of sieve
Speed is 21m/s, obtains cotton benevolence；
C. cotton benevolence is moved into softening kettle and is softened, the temperature of cotton benevolence is 85 DEG C after softening；Again with flaker by cotton benevolence
Embryo is rolled, obtained embryo piece thickness is 0.25mm, and embryo piece is then carried out low temperature drying with plate-press dryer, and temperature when drying is
70℃；Embryo piece after drying moves into tow chain type leacher, first carries out degreasing with 6# oil, again with methanol solution carries out dephenolize, used
A concentration of the 53% of methanol solution, the mass volume ratio of embryo piece and methanol solution is 1t after drying:0.6m3；
D. the wet cotton dregs of dephenolize are obtained after dephenolize, wet cotton dregs are first dried, then carry out precipitation, then crush,
Screening, the fines sifted out, which enters, turns sieve, and the aperture for turning sieve transfer sieve plate is 18 mesh, and the rotating speed of rotating cylinder is 45r/min, by cotton
Shell, bobbles and Cottonseed Meal screen out, and obtain cottonseed protein, and cottonseed protein is measured and packed.
Advantage to illustrate the invention, the cottonseed protein that Examples 1 to 3 and check experiment are produced are carried out into sorting
It surveys, testing result is as shown in table 1.
Table 1
As can be seen from Table 1, the protein content for the cottonseed protein that the embodiment of the present invention 1~3 produces is 66% or so, long-range
In 35% protein content of check experiment；Cotton oil amount is respectively less than 0.4%, and the cotton oil amount of check experiment is up to 0.95%；Cotton
Phenol content is 166~183ppm, and check experiment is up to 236ppm；Coarse-fibred content be 6.2~6.6wt%, much smaller than pair
According to the crude fiber content of experiment 16wt%；Potash hydroxide solubility is 59~65, molten much larger than the potassium hydroxide of check experiment 41
Xie Du；Cinder is divided into 4~6%, is much smaller than the crude ash content of check experiment 12%；Solvent for use is ethanol solution when dephenolize,
Compared with the methanol solution of check experiment, operational risk smaller when production obtains product safety higher；Examples 1 to 3 is de-
Fat, dephenolize can be carried out at the same time, and compared with check experiment degreasing, dephenolize must be carried out separately, simplified production technology, improved production
Efficiency；In addition, it is 1g with the mass ratio for drying embryo piece that Examples 1 to 3 degreasing dephenolize process, which utilizes complex enzyme, complex enzyme,:5~
10kg, enzyme dosage used is small, and the use of enzyme can be by the more complete of cell degradation so that albumen, gossypol, cotton oil etc. can fill
Divide and release, to further decrease the content of gossypol, cotton oil etc., and check experiment does not use complex enzyme, gossypol, cotton oil etc.
Removal is incomplete, and cottonseed protein content is low.In summary, cottonseed protein quality produced by the invention is high, so as to promote cottonseed
The feed value of albumen, the purposes range for widening cottonseed protein.
Claims (9)
1. a kind of production method of cottonseed protein, including clean, peel off, being crushed, rolling embryo, degreasing dephenolize, precipitation, drying process,
It is characterized in that：Further include that hair light seed is subjected to pickling；Complex enzyme is added in degreasing dephenolize.
2. a kind of production method of cottonseed protein according to claim 1, it is characterised in that include the following steps：
A. gross cottonseed cleaned, shell suede, obtain a mao light seed；
B. hair light seed is subjected to pickling, obtains light seed；
C. light seed peeled off, be crushed, then impurity elimination, obtain cotton benevolence；
D. cotton benevolence is carried out obtaining embryo piece after rolling embryo, then drying, degreasing dephenolize, obtain the wet cotton dregs of dephenolize；
E. the wet cotton dregs of dephenolize are crushed after drying, precipitation, are sieved, and the fines sifted out is sorted again, obtains cottonseed protein.
3. a kind of production method of cottonseed protein according to claim 2, it is characterised in that：The complex enzyme includes fiber
The mass ratio of plain enzyme and lignin oxidation's enzyme, complex enzyme and the embryo piece after drying is 1g:5~10kg, cellulase and lignin
The mass ratio of oxidizing ferment is 1:1~1.2.
4. a kind of production method of cottonseed protein according to claim 2, it is characterised in that：The acid solution used when pickling is
The mass volume ratio of sulfuric acid solution, a concentration of 0.12~0.13mol/L of sulfuric acid solution, hair light seed and sulfuric acid solution is 100g:
The time of 0.15~0.2L, pickling are 10~15min.
5. a kind of production method of cottonseed protein according to claim 2, it is characterised in that：Throwing is used when sieving impurity elimination
Sieve, the material casting speed for throwing sieve are 18~21m/s.
6. a kind of production method of cottonseed protein according to claim 2, it is characterised in that：Proembryo is rolled first by cotton benevolence soft
Change and softened in pot, temperature is 82~88 DEG C after softening；The thickness of the embryo piece is 0.2~0.3mm.
7. a kind of production method of cottonseed protein according to claim 2, it is characterised in that：The dephenolize is molten using ethyl alcohol
Liquid is handled, and a concentration of the 50~55% of ethanol solution, the mass volume ratio of embryo piece and ethanol solution after drying is 1t:
0.4~0.7m3。
8. a kind of production method of cottonseed protein according to claim 2, it is characterised in that：Drying is to use plate-press dryer
It is dried at 65~75 DEG C, degreasing, dephenolize are carried out at the same time using the tow chain type leacher of strip wing, degreasing, dephenolize
When temperature be 40~55 DEG C.
9. a kind of production method of cottonseed protein according to claim 2, it is characterised in that：Sorting is carried out using a turn sieve
, the aperture for turning sieve transfer sieve plate is 16~20 mesh, and the rotating speed of rotating cylinder is 30~60r/min.
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| CN110437300A (en) * | 2019-08-16 | 2019-11-12 | 晨光生物科技集团喀什有限公司 | A kind of production technology recycling cotton oil, cotton protein in native suede |
| CN112358912A (en) * | 2020-09-29 | 2021-02-12 | 新疆希普生物科技股份有限公司 | Preparation method and application of baked cottonseed |
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