CN108353925A - A kind of Compound Streptomycin bactericide - Google Patents
A kind of Compound Streptomycin bactericide Download PDFInfo
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- CN108353925A CN108353925A CN201810264631.2A CN201810264631A CN108353925A CN 108353925 A CN108353925 A CN 108353925A CN 201810264631 A CN201810264631 A CN 201810264631A CN 108353925 A CN108353925 A CN 108353925A
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- Prior art keywords
- compound
- bactericide
- streptomycin
- auxiliary material
- rice
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
- A01N47/44—Guanidine; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/82—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with three ring hetero atoms
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A kind of Compound Streptomycin bactericide, it is characterized in that the drug is 1 by least one auxiliary material and mass ratio:0.3~0.5 streptomycin sulphate and 3 O (2 base of N (5 ((4 methoxyphenyl) sulfydryl) 1,3,4 thiadiazoles, 2 base) acetamide) 3 ', 4 ', 5 ', 5,7 pentamethoxyl myricetins (A10) form.The dosage form of the bactericide is wettable powder, and the auxiliary material is the auxiliary material suitable for wettable powder, including dispersant, wetting agent, suspending agent and carrier.The preferred naphthalenesulfonic acid-formaldehyde condensate of dispersant, wetting agent preferably sodium dodecyl sulfate, the preferred hydroxypropyl methyl cellulose of suspending agent, the carrier are selected from least one of attapulgite, diatomite, kaolin, talcum powder, starch, glucose, sodium sulphate, precipitated calcium carbonate, white carbon.
Description
Technical field
The present invention relates to a kind of rice diseases to prevent medication.
Background technology
Bacterial blight of rice is a kind of bacteriosis, is one of three major disease of China's rice, occurs mainly in East China, China
In, South China rice region, be also distributed in northwest, southwest, North, Northeast China part rice region.Bacterial blight of rice pathogen ownership is false single
Born of the same parents bacillus, Xanthomonas.The rice underproduction infected is up to 50-60%, or even total crop failure.Streptomycin sulphate is as a kind of amino
Glycoside antibiotic then can be used for preventing the various bacteria and fungoid disease including bacterial blight of rice in pesticide field
Evil, such as 15%~20% streptomysin wettable powder of pesticide, soluble powder and 72% agricultural streptomycin original
Expect medicine.But under long-time service, bacterial blight of rice germ has produced stronger resistance to streptomysin, and due to streptomysin
Can also harm be generated to environment and human and livestock health, can not be made by increasing with the residual in plant in the environment as antibiotic
Dosage come keep prevention bacterial blight of rice effect.
Under study for action it was found that disclosing a series of amide containing thioether thiadiazoles in Chinese patent literature CN107602548A
Myricetin derivative, and disclose it and can be used for preparing disinfectant use in agriculture and antivirotic.The document specifically discloses 3-
O- (N- (5- ((4- methoxyphenyls) sulfydryl) -1,3,4- thiadiazoles -2- bases)-acetamide -2- bases) -3 ', 4 ', 5 ', 5,7- five
Methoxyl group myricetin (A10) is although compound (A10) shows the prevention certain to bacterial blight of rice in isolated experiment
Effect, but effect is similar with traditional plant fungicide Yekuzuo, can not show a candle to Huang Dezhi Thiodiazole-copper.Therefore in existing skill
On the basis of art, based on the above problem in the prior art, compound plant bactericide how is rationally formed with streptomysin to improve rice
The rice diseases control effect such as bacterial leaf-blight becomes urgent problem to be solved in the prior art.
Invention content
Under study for action it was found that using 3-O- (N- (5- ((4- methoxyphenyls) sulfydryl) -1,3,4- thiadiazoles -2-
Base)-acetamide -2- bases) -3 ', 4 ', 5 ', 5,7- pentamethoxyl myricetins (A10) and streptomysin compatibility be when being made compound, and it can
The significant control effect improved for plant diseases such as bacterials blight of rice, is based on this, the present invention provides following technical sides
Case：
A kind of Compound Streptomycin bactericide, it is characterized in that the drug is 1 by least one auxiliary material and mass ratio:
0.3~0.5 streptomycin sulphate and 3-O- (N- (5- ((4- methoxyphenyls) sulfydryl) -1,3,4- thiadiazoles -2- bases)-acetyl
Amine -2- bases) -3 ', 4 ', 5 ', 5,7- pentamethoxyls myricetin (A10) composition.
A kind of Compound Streptomycin bactericide, it is characterized in that sulfate chain in the rice disease protective agents
Mycin and the mass ratio of compound (A10) are 1:0.35~0.45.
A kind of Compound Streptomycin bactericide, it is characterized in that the dosage form of the rice disease protective agents is selected from
Wettable powder, suspending agent, soluble powder.Streptomycin sulphate and the gross mass of compound (A10) account for the total matter of bactericide
The 15%~30% of amount.
A kind of Compound Streptomycin bactericide, it is characterized in that the dosage form of the bactericide is wettable powder
Agent, the auxiliary material are the auxiliary material suitable for wettable powder, including dispersant, wetting agent, suspending agent and carrier.
The dispersant is selected from APES formaldehyde condensation products sulfat (SOPA), Nekal BX, naphthalene sulfonic acids
Sodium formaldehyde condensation product (NNO), one kind in sodium lignin sulfonate；
The wetting agent is in lauryl sodium sulfate, sodium alkyl benzene sulfonate, Negel formaldehyde condensation products
It is one or more.
The suspending agent is selected from the one or more of polyethylene glycol, sodium carboxymethylcellulose, hydroxypropyl methyl cellulose.
The carrier is selected from attapulgite, diatomite, kaolin, talcum powder, starch, glucose, sodium sulphate, lightweight carbonic acid
At least one of calcium, white carbon.
A kind of Compound Streptomycin bactericide, it is characterized in that the preferred naphthalenesulfonic acid-formaldehyde condensate of dispersant, profit
Humectant preferably sodium dodecyl sulfate, the preferred hydroxypropyl methyl cellulose of suspending agent.
Compound Streptomycin bactericide provided by the invention, by by streptomycin sulphate with compound (A10) with specific
Ratio carries out compatibility, significantly improves the bacteriostatic activity to bacterial blight of rice pathogenic bacteria, makes its control effect and be administered alone
Streptomysin or compound (A10), which are compared, to be significantly increased.So as on the basis of reaching same control effect, significantly drop
The usage amount of low streptomycin sulphate, and then reduce pollution of the streptomycin sulphate environment and the bad shadow caused by user health
It rings.In addition it, which belongs to low toxicity compounds, is shown to the detection of the acute toxicity of compound (A10).It is found in recipe determination, sulfate chain
The proportioning of mycin and compound (A10) is 1:When 0.3~0.5, more apparent raising prevention bacterial blight of rice can be played
The effect of effect, higher or lower compound (A10) addition can not all generate this effect, and by streptomycin sulphate and chemical combination
The ratio of object (A10) is preferably 1:When 0..35~0.45, the effect for improving prevention bacterial blight of rice becomes apparent, and
In advance it was found that when withered using the other common bacterial blight of rice protective agents such as leaves of compound (A10) in option screening
When azoles is with compound (A10) compatibility, apparent effect is had no.
Specific implementation mode
One, in vitro antibacterial activity test
(1)) test method
Using nephelometry, each experimental drug is tested to rice leaf spot bacteria
The inhibitory activity of (Xanthomonasoryzaepv.oryzae (Xoo)), concrete operation step are as follows：
A. in 2000mL beakers be added 1000mL sterile purified waters, sequentially added under electromagnetic agitation peptone 5.0g,
Yeast powder 1.0g, glucose 10.0g, beef extract 3.0g, it is to be mixed uniformly after pH adjusted with sodium hydrate aqueous solution
To neutral (7.2 ± 0.2)；
B. test tube sterilizing is cleaned to be placed on rack for test tube, using liquid-transfering gun into every test tube solution in removing step A
After 4.0mL plus rubber stopper, every 6 test tubes packaging is primary, after the 20min that sterilize at 121 DEG C using autoclave for use；
C. 0.00375-0.0042g samples to be tested are weighed in centrifuge tube, to pipette 80 μ L respectively after 150 μ LDMSO dissolvings
With in numbered centrifuge tube after 40 μ L to sterilizing, separately add 40 μ LDMSO to equipped with 40 μ L sample solution centrifuge tube, upwards
It states and 4mLTween-20 is respectively added in centrifuge tube, while setting Thiodiazole-copper and Yekuzuo compares medicament, DMSO makees blank.Per fragmented
Solution, which pipettes in the sterile medium test tube of 1mL to 3 dress step B, in heart pipe (operates before alcolhol burner, prevents other bacteriums dirty
Dye)；
E. 96 orifice plate of blank is taken, blank OD values is surveyed and excludes the hole that OD values are more than 0.05,200 to be added in backward each available hole
Solution is surveyed OD values and is recorded in test tube in μ L (4), and the rice leaf spot bacteria after 40 μ L activation is finally accessed into every test tube
Strain, 24~48h of shaken cultivation in 30 DEG C, 180rpm constant-temperature tables, during which in test tube solution O D values to track bacterium
Growth conditions take 200 μ L solution to survey OD values and record in test tube after culture；
F. Bacteria suppression rate calculation formula is as follows,
Correct the OD values=values of OD containing bacterium culture medium-aseptic culture medium OD values
Sample to be tested grouping is as follows with when experimental result：(n=3)
The compound (A10) used in experiment is provided by Tianjin Kai Yin Science and Technology Ltd.s, and >=98%, lot number is content
20170905, Thiodiazole-copper uses 20% suspending agent, and drug dose to be measured is calculated with active constituent actual content in each experimental group.
The result shows that it is 14 to match：6 to 15：5 streptomycin sulphate shows better bacteriostatic activity with compound (A10), wherein
14:6 experimental group 3 is not only higher than the experimental group 6/7 that two kinds of drugs are used alone, also higher than existing mature plant fungicide leaf
Withered azoles and Huang Dezhi Thiodiazole-copper.
Two, embodiment
A kind of Compound Streptomycin bactericide provided by the invention, prepare become wettable powder, by streptomycin sulphate,
Compound (A10) and auxiliary material composition.The formula of the composition prepares comparison respectively as shown in Examples 1 to 5 in following table
Example 1~5 (percentage shown in table is the mass percent for accounting for composition gross mass, disregards moisture)：
The preparation method of the composition is：
1) streptomycin sulphate crushes respectively with compound (A10),
2) by dispersant, wetting agent, mixed with suspending agent after grind, the obtained active constituent powder of step 1) is added,
It adds after carrier mixes well and crosses 200 mesh sieve；Obtain wettable powder (moisture content ＜ 1%).
The preferred NNO of dispersant, wetting agent preferably sodium dodecyl sulfate, the preferred hydroxypropyl methyl cellulose of suspending agent carry
Body is weight ratio 100：5 kaolin and talcum powder.
Three, control effect is tested
With field leaf-cutting connect bacterium method for measuring test different proportion streptomycin sulphate and compound (A10) it is white to rice
The control effect of leaf blight, experimental procedure are as follows
1, using the rice leaf spot bacteria strain of Zhejiang Academy of Agricultural Science plant protection and the offer of institute of microbiology
(Xanthomonasoryzaepv.oryzae (Xoo)), culture become 109After the suspension of a/ml, with the dissecting scissors for being moistened with bacterium solution
3~4cm of rice blade tip, 20 rice leaves of every group of selection are subtracted, the spray prevention in 7 days after connecing bacterium is examined or check and compareed for 10 days after administration
The control effect (calculating control effect with Spot expansion) and use Excel that group is compared carry out t inspections, water used to experimental result
Rice varieties are middle morning 39 (authorization number is that rice 2009039 is examined in Zhejiang)
2, grouping see the table below (means ± s, n=20) with administration with effect situation
By above-mentioned each group drug, experimental group 1~7 and control 1~2 are by wettable powder made from each embodiment and comparative example
It is diluted according to active ingredient final concentration 200ppm, control 3 is using commercially available 20% Thiodiazole-copper suspending agents according to active ingredient end
Concentration 200ppm is diluted, and positive controls are diluted with water 1000 times using the suspending agent (active substance) of comparative example 5.
Can be seen that by above-mentioned experiment the result shows that, the preferred sulphur of bactericide that the embodiment of the present invention 1~5 provides
The ratio of sour streptomysin and compound (A10) is 1:0.3~0.5, compared with streptomycin sulphate or compound (A10) is applied alone,
The effect of prevention bacterial blight of rice prevention can be improved.The control effect compared with compareing 1,2 group of experimental group 1~5 has significantly
It improves (P ＜ 0.05).Using the experimental group 6/7 of comparative example 1/2, control effect has been decreased obviously.Illustrate streptomycin sulphate
Synergistic effect, and sulfuric acid in embodiment 2~4 are produced under the ratio selected in the technical solution of the present invention with compound (A10)
The preferred proportion (1 of streptomysin and compound (A10)：0.35~0.45) it, can be generated when preventing rice bacterial leaf spot preferably
Control effect.
Claims (5)
1. a kind of Compound Streptomycin bactericide, it is characterized in that the drug is 1 by least one auxiliary material and mass ratio:0.3
~0.5 streptomycin sulphate and 3-O- (N- (5- ((4- methoxyphenyls) sulfydryl) -1,3,4- thiadiazoles -2- bases)-acetamide -
2- yls) -3 ', 4 ', 5 ', 5,7- pentamethoxyls myricetin (A10) composition.
2. a kind of Compound Streptomycin bactericide as described in claim 1, it is characterized in that the rice disease prevents medicine
Streptomycin sulphate and the mass ratio of compound (A10) are 1 in object:0.35~0.45.
3. a kind of Compound Streptomycin bactericide as claimed in claim 1 or 2, it is characterized in that the rice disease prevents medicine
The dosage form of object is selected from wettable powder, suspending agent, soluble powder, and streptomycin sulphate and the gross mass of compound (A10) account for plant
The 15%~30% of object fungicide gross mass.
4. a kind of Compound Streptomycin bactericide as described in claim 1, it is characterized in that the dosage form of the bactericide
For wettable powder, the auxiliary material is the auxiliary material suitable for wettable powder, including dispersant, wetting agent, suspending agent and carrier.
5. a kind of Compound Streptomycin bactericide as claimed in claim 4, it is characterized in that the preferred sodium naphthalene sulfonate first of dispersant
Aldehyde condensate, wetting agent preferably sodium dodecyl sulfate, the preferred hydroxypropyl methyl cellulose of suspending agent, the carrier are selected from bumps
At least one of stick soil, diatomite, kaolin, talcum powder, starch, glucose, sodium sulphate, precipitated calcium carbonate, white carbon.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810264631.2A CN108353925A (en) | 2018-03-28 | 2018-03-28 | A kind of Compound Streptomycin bactericide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810264631.2A CN108353925A (en) | 2018-03-28 | 2018-03-28 | A kind of Compound Streptomycin bactericide |
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| Publication Number | Publication Date |
|---|---|
| CN108353925A true CN108353925A (en) | 2018-08-03 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810264631.2A Pending CN108353925A (en) | 2018-03-28 | 2018-03-28 | A kind of Compound Streptomycin bactericide |
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| Country | Link |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102239873A (en) * | 2011-05-17 | 2011-11-16 | 吴元林 | Bactericide composite containing streptomycin sulphate and application thereof |
| CN107517995A (en) * | 2017-08-18 | 2017-12-29 | 广东省农业科学院农业生物基因研究中心 | A kind of formula and preparation method and application for improving hybrid rice seeds storage capacity |
| CN107602548A (en) * | 2017-09-14 | 2018-01-19 | 贵州大学 | A kind of myricetin derivative, the Preparation method and use of amide containing thioether thiadiazoles |
-
2018
- 2018-03-28 CN CN201810264631.2A patent/CN108353925A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102239873A (en) * | 2011-05-17 | 2011-11-16 | 吴元林 | Bactericide composite containing streptomycin sulphate and application thereof |
| CN107517995A (en) * | 2017-08-18 | 2017-12-29 | 广东省农业科学院农业生物基因研究中心 | A kind of formula and preparation method and application for improving hybrid rice seeds storage capacity |
| CN107602548A (en) * | 2017-09-14 | 2018-01-19 | 贵州大学 | A kind of myricetin derivative, the Preparation method and use of amide containing thioether thiadiazoles |
Non-Patent Citations (1)
| Title |
|---|
| 杨平华 等: "《农田常用杀菌剂使用技术》", 31 January 2009, 四川科学技术出版社 * |
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Application publication date: 20180803 |