CN108402069A - A kind of bactericide for preventing rice disease - Google Patents
A kind of bactericide for preventing rice disease Download PDFInfo
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- CN108402069A CN108402069A CN201810266023.5A CN201810266023A CN108402069A CN 108402069 A CN108402069 A CN 108402069A CN 201810266023 A CN201810266023 A CN 201810266023A CN 108402069 A CN108402069 A CN 108402069A
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- Prior art keywords
- bactericide
- rice disease
- compound
- streptomycin sulphate
- rice
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
- A01N47/44—Guanidine; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/82—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with three ring hetero atoms
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A kind of bactericide for preventing rice disease is 1 by least one auxiliary material and mass ratio:0.25~0.35 streptomycin sulphate and 3 O (2 base of N (5 ((phenyl) sulfydryl) 1,3,4 thiadiazoles, 2 base) acetamide) 3 ', 4 ', 5 ', 5,7 pentamethoxyl myricetins (A2) form.The dosage form of the rice disease protective agents is selected from wettable powder, suspending agent, soluble powder.Streptomycin sulphate and the gross mass of compound (A2) account for the 15%~30% of bactericide gross mass.The dosage form of the bactericide is wettable powder, and the auxiliary material is the auxiliary material suitable for wettable powder, including dispersant, wetting agent, suspending agent and carrier.The preferred Nekal BX of dispersant, wetting agent preferably sodium dodecyl sulfate, suspending agent preferably carboxymethyl cellulose sodium.
Description
Technical field
The present invention relates to a kind of rice diseases to prevent medication.
Background technology
Bacterial blight of rice is a kind of bacteriosis, is one of three major disease of China's rice, occurs mainly in East China, China
In, South China rice region, be also distributed in northwest, southwest, North, Northeast China part rice region.Bacterial blight of rice pathogen ownership is false single
Born of the same parents bacillus, Xanthomonas.The rice underproduction infected is up to 50-60%, or even total crop failure.Streptomycin sulphate is as a kind of amino
Glycoside antibiotic is used for prevention lungy in people's veterinary medicine field, and then can be used for preventing in pesticide field includes rice
Various bacteria including bacterial leaf-blight and fungal disease.Due to using for many years, existing bacterial blight of rice germ is to strepto-
The better resistance of element, and streptomysin is as a kind of antibiotic, it in the environment also can be to environment and people and animals with the residual in plant
Health generates harm, can not keep effect of the streptomysin when preventing bacterial blight of rice by increasing usage amount.
Based on problem in the prior art above, how rationally forming compound, a kind of novel can to improve rice white to provide
The bactericide of the rice diseases control effect such as leaf blight becomes urgent problem to be solved in the prior art.
Invention content
To solve aforementioned technical problem, under study for action it was found that disclosing one in Chinese patent literature CN107602548A
The myricetin derivative of serial amide containing thioether thiadiazoles, and disclose it and can be used for preparing disinfectant use in agriculture and antiviral
Agent.The document specifically discloses 3-O- (N- (5- ((phenyl) sulfydryl) -1,3,4- thiadiazoles -2- bases)-acetamide -2- bases) -3 ',
4 ', 5 ', 5,7- pentamethoxyl myricetin (A2).According to content disclosed in CN107602539A, compound (A2) is to rice
Bacterial leaf-blight bacterial strain carries out in vitro bacteriostatic experiment room and shows certain bacteriostatic activity, and bacteriostatic activity is between Thiodiazole-copper and leaf
Between withered azoles.We have found under study for action, using 3-O- (N- (- 1,3,4- thiadiazoles -2- bases of 5- ((phenyl) sulfydryl))-acetyl
Amine -2- bases) -3 ', 4 ', 5 ', 5,7- pentamethoxyl myricetins (A2) and streptomysin compatibility when compound is made, can improve significantly
For the control effect of the plant diseases such as bacterial blight of rice, it is based on this, the present invention provides following technical schemes：
A kind of bactericide for preventing rice disease, it is characterized in that the drug is by least one auxiliary material and quality
Than being 1:0.25~0.35 streptomycin sulphate and 3-O- (N- (5- ((phenyl) sulfydryl) -1,3,4- thiadiazoles -2- bases)-acetyl
Amine -2- bases) -3 ', 4 ', 5 ', 5,7- pentamethoxyls myricetin (A2) composition.
A kind of bactericide for preventing rice disease, it is characterized in that the rice disease protective agents
Middle streptomycin sulphate and the mass ratio of compound (A2) are 1:0.19~0.23.
A kind of bactericide for preventing rice disease, it is characterized in that the rice disease protective agents
Dosage form is selected from wettable powder, suspending agent, soluble powder.Streptomycin sulphate and the gross mass of compound (A2) account for plant sterilization
The 15%~30% of agent gross mass, preferably sulfuric acid streptomysin and the gross mass of compound (A2) account for the 15% of the bactericide
~20%.
A kind of bactericide for preventing rice disease, it is characterized in that the dosage form of the bactericide is
Wettable powder, the auxiliary material are the auxiliary material suitable for wettable powder, including dispersant, wetting agent, suspending agent and carrier.
The dispersant is selected from APES formaldehyde condensation products sulfat (SOPA), Nekal BX, naphthalene sulfonic acids
Sodium formaldehyde condensation product (NNO), one kind in sodium lignin sulfonate；
The wetting agent is in lauryl sodium sulfate, sodium alkyl benzene sulfonate, Negel formaldehyde condensation products
It is one or more.
The suspending agent is selected from the one or more of polyethylene glycol, sodium carboxymethylcellulose, hydroxypropyl methyl cellulose.
The carrier is selected from attapulgite, diatomite, kaolin, talcum powder, starch, glucose, sodium sulphate, lightweight carbonic acid
At least one of calcium, white carbon.
A kind of bactericide for preventing rice disease, it is characterized in that the preferred Nekal BX of dispersant, wetting
Agent preferably sodium dodecyl sulfate, suspending agent preferably carboxymethyl cellulose sodium.
It, can be notable under study for action it was found that streptomycin sulphate and compound (A2) are carried out compatibility with special ratios
Improve the bacteriostatic activity to bacterial blight of rice pathogenic bacteria.And it, which belongs to low toxicityization, is shown to the detection of the acute toxicity of compound (A2)
Close object.Based on the discovery, the present invention provides a kind of bactericides for preventing rice disease, pass through preferably sulfuric acid strepto-
The ratio of element and compound (A2), makes its control effect have compared with streptomysin or compound (A2) is administered alone and significantly carries
It is high.So as on the basis of reaching same control effect, significantly reduce the usage amount of streptomycin sulphate, and then reduce sulfuric acid
Pollution of the streptomysin environment and the harmful effect caused by user health.We have found in recipe determination, sulfuric acid strepto-
The proportioning of element and compound (A2) is 1:When 0.25~0.35, more apparent raising prevention bacterial blight of rice effect can be played
The effect of fruit, higher or lower compound (A2) addition can not all generate this effect, and by streptomycin sulphate and compound
(A2) ratio is preferably 1:When 0.28~0.32, the effect for improving prevention bacterial blight of rice becomes apparent, and in advance
It was found that compound (A2) and the synergistic effect of streptomycin sulphate have specificity, when using other normal in option screening
When with bacterial blight of rice protective agents such as Yekuzuo and compound (A2) compatibility, apparent effect is had no.
Specific implementation mode
One, in vitro antibacterial activity test
(1) test method
Using nephelometry, each experimental drug is tested to rice leaf spot bacteria
The inhibitory activity of (Xanthomonasoryzaepv.oryzae (Xoo)), concrete operation step are as follows：
A. in 2000mL beakers be added 1000mL sterile purified waters, sequentially added under electromagnetic agitation peptone 5.0g,
Yeast powder 1.0g, glucose 10.0g, beef extract 3.0g, it is to be mixed uniformly after pH adjusted with sodium hydrate aqueous solution
To neutral (7.2 ± 0.2)；
B. test tube sterilizing is cleaned to be placed on rack for test tube, using liquid-transfering gun into every test tube solution in removing step A
After 4.0mL plus rubber stopper, every 6 test tubes packaging is primary, after the 20min that sterilize at 121 DEG C using autoclave for use；
C. 0.00375-0.0042g samples to be tested are weighed in centrifuge tube, to pipette 80 μ respectively after 150 μ L DMSO dissolvings
After L and 40 μ L to sterilizing in numbered centrifuge tube, 40 μ LDMSO are separately added to the centrifuge tube equipped with 40 μ L sample solution, upwards
It states and 4mLTween-20 is respectively added in centrifuge tube, while setting Thiodiazole-copper and Yekuzuo compares medicament, DMSO makees blank.Per fragmented
Solution, which pipettes in the sterile medium test tube of 1mL to 3 dress step B, in heart pipe (operates before alcolhol burner, prevents other bacteriums dirty
Dye)；
E. 96 orifice plate of blank is taken, blank OD values is surveyed and excludes the hole that OD values are more than 0.05,200 to be added in backward each available hole
Solution is surveyed OD values and is recorded in test tube in μ L (4), and the rice leaf spot bacteria after 40 μ L activation is finally accessed into every test tube
Strain, 24~48h of shaken cultivation in 30 DEG C, 180rpm constant-temperature tables, during which in test tube solution O D values to track bacterium
Growth conditions take 200 μ L solution to survey OD values and record in test tube after culture；
F. Bacteria suppression rate calculation formula is as follows,
Correct the OD values=values of OD containing bacterium culture medium-aseptic culture medium OD values
Sample to be tested grouping is as follows with when experimental result：(n=3)
The compound (A2) used in experiment is provided by Tianjin Kai Yin Science and Technology Ltd.s, and >=98%, lot number is content
20170905, Thiodiazole-copper uses 20% suspending agent, and drug dose to be measured is calculated with active constituent actual content in each experimental group.
The result shows that it is 15 to match：5 to 16:4 streptomycin sulphate shows better bacteriostatic activity with compound (A2), not only
Higher than the experimental group that two kinds of drugs are used alone, also higher than existing mature plant fungicide Yekuzuo and Huang Dezhi
Thiodiazole-copper.
Two, embodiment
A kind of bactericide for preventing rice disease provided by the invention, preparing becomes wettable powder, by sulphur
Sour streptomysin, compound (A2) and auxiliary material composition.The formula of the composition is respectively as shown in Examples 1 to 5 in following table, simultaneously
Prepare comparative example 1~5 (percentage shown in table is the mass percent for accounting for composition gross mass, disregards moisture)：
The preparation method of the composition is：
1) streptomycin sulphate crushes respectively with compound (A2),
2) by dispersant, wetting agent, mixed with suspending agent after grind, the obtained active constituent powder of step 1) is added,
It adds after carrier mixes well and crosses 200 mesh sieve；Obtain wettable powder (moisture content ＜ 1%).
The preferred Nekal BX of dispersant, wetting agent preferably sodium dodecyl sulfate, suspending agent preferably carboxymethyl cellulose sodium,
Carrier is weight ratio 10：1 attapulgite and white carbon.
Three, control effect is tested
With field leaf-cutting connect bacterium method for measuring test different proportion streptomycin sulphate and compound (A2) it is white to rice
The control effect of leaf blight, experimental procedure are as follows
1, using the rice leaf spot bacteria strain of Zhejiang Academy of Agricultural Science plant protection and the offer of institute of microbiology
(Xanthomonasoryzaepv.oryzae (Xoo)), culture become 109After the suspension of a/ml, with the dissecting scissors for being moistened with bacterium solution
3~4cm of rice blade tip is subtracted, 20 rice leaves of every group of selection, 7 days sprays prevent after connecing bacterium, examine or check and compare within 10 days after administration
The control effect (calculating control effect with Spot expansion) and use Excel that group is compared carry out t inspections, water used to experimental result
Rice varieties are middle morning 39 (authorization number is that rice 2009039 is examined in Zhejiang)
2, grouping see the table below (means ± s, n=20) with administration with effect situation
By above-mentioned each group drug, experimental group 1~7 and control 1~2 are by wettable powder made from each embodiment and comparative example
It is diluted according to active ingredient final concentration 200ppm, control group 3 is using commercially available 20% Thiodiazole-copper suspending agents according to active ingredient
Final concentration 200ppm is diluted.Positive controls are diluted with water 1000 times using the suspending agent (active substance) of comparative example 5.
The experimental results showed that the bactericide that the embodiment of the present invention 1~5 provides, and is applied alone streptomycin sulphate or chemical combination
Object (A2) is compared, and effect when being prevented for bacterial blight of rice is improved.Experimental group 1~5 and pair for using streptomycin sulphate
It is significantly increased (P ＜ 0.05) compared to control effect according to 1 group.And the experimental group 6/7 of comparative example 1/2 is used, although with experiment
The active ingredient of group 1~5 is closer to, but its control effect has been decreased obviously.Illustrate streptomycin sulphate and compound (A2)
In the technical solution of the present invention synergistic effect, and streptomycin sulphate and compound in embodiment 2~4 are produced under selected ratio
(A2) preferred proportion can generate better control effect when preventing rice bacterial leaf spot.
Claims (6)
1. a kind of bactericide for preventing rice disease, it is characterized in that the drug is by least one auxiliary material and mass ratio
It is 1:0.25~0.35 streptomycin sulphate and 3-O- (N- (5- ((phenyl) sulfydryl) -1,3,4- thiadiazoles -2- bases)-acetamide -
2- yls) -3 ', 4 ', 5 ', 5,7- pentamethoxyls myricetin (A2) composition.
2. a kind of bactericide for preventing rice disease as described in claim 1, it is characterized in that the rice disease
Streptomycin sulphate and the mass ratio of compound (A2) are 1 in evil protective agents:0.19~0.23.
3. a kind of bactericide for preventing rice disease as claimed in claim 1 or 2, it is characterized in that the rice is sick
The dosage form of evil protective agents is selected from wettable powder, suspending agent, soluble powder.Total matter of streptomycin sulphate and compound (A2)
Amount accounts for the 15%~30% of bactericide gross mass.
4. a kind of bactericide for preventing rice disease as claimed in claim 3, it is characterized in that preferably sulfuric acid strepto-
Element and the gross mass of compound (A2) account for the 15%~20% of the bactericide.
5. a kind of bactericide for preventing rice disease as claimed in claim 4, it is characterized in that the plant sterilization
The dosage form of agent is wettable powder, and the auxiliary material is the auxiliary material suitable for wettable powder, including dispersant, wetting agent, suspending
Agent and carrier.
6. a kind of bactericide for preventing rice disease as claimed in claim 5, it is characterized in that dispersant preferably pulls open
Powder BX, wetting agent preferably sodium dodecyl sulfate, suspending agent preferably carboxymethyl cellulose sodium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810266023.5A CN108402069A (en) | 2018-03-28 | 2018-03-28 | A kind of bactericide for preventing rice disease |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810266023.5A CN108402069A (en) | 2018-03-28 | 2018-03-28 | A kind of bactericide for preventing rice disease |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108402069A true CN108402069A (en) | 2018-08-17 |
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ID=63132593
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810266023.5A Pending CN108402069A (en) | 2018-03-28 | 2018-03-28 | A kind of bactericide for preventing rice disease |
Country Status (1)
| Country | Link |
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| CN (1) | CN108402069A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105211075A (en) * | 2014-05-30 | 2016-01-06 | 四川利尔作物科学有限公司 | Bactericidal composition and application thereof |
| CN107602548A (en) * | 2017-09-14 | 2018-01-19 | 贵州大学 | A kind of myricetin derivative, the Preparation method and use of amide containing thioether thiadiazoles |
-
2018
- 2018-03-28 CN CN201810266023.5A patent/CN108402069A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105211075A (en) * | 2014-05-30 | 2016-01-06 | 四川利尔作物科学有限公司 | Bactericidal composition and application thereof |
| CN107602548A (en) * | 2017-09-14 | 2018-01-19 | 贵州大学 | A kind of myricetin derivative, the Preparation method and use of amide containing thioether thiadiazoles |
Non-Patent Citations (1)
| Title |
|---|
| 杨平华 主编: "《农田常用杀菌剂使用技术》", 31 January 2009 * |
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Application publication date: 20180817 |
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