CN108350447B - 启动子变体 - Google Patents

启动子变体 Download PDF

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Publication number
CN108350447B
CN108350447B CN201680058388.8A CN201680058388A CN108350447B CN 108350447 B CN108350447 B CN 108350447B CN 201680058388 A CN201680058388 A CN 201680058388A CN 108350447 B CN108350447 B CN 108350447B
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promoter
seq
poi
sequence
fold
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Chinese (zh)
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CN108350447A (zh
Inventor
D·马塔诺维赫
B·加塞尔
R·普里尔霍弗
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Lonza AG
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Lonza AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
CN201680058388.8A 2015-08-05 2016-08-05 启动子变体 Active CN108350447B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP2015068024 2015-08-05
EPPCT/EP2015/068024 2015-08-05
EP16163932 2016-04-05
EP16163932.3 2016-04-05
PCT/EP2016/068784 WO2017021541A1 (en) 2015-08-05 2016-08-05 Promoter variants

Publications (2)

Publication Number Publication Date
CN108350447A CN108350447A (zh) 2018-07-31
CN108350447B true CN108350447B (zh) 2021-08-31

Family

ID=56684642

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201680058388.8A Active CN108350447B (zh) 2015-08-05 2016-08-05 启动子变体

Country Status (13)

Country Link
US (2) US10752907B2 (https=)
EP (1) EP3332005B1 (https=)
JP (1) JP6833812B2 (https=)
KR (1) KR102253479B1 (https=)
CN (1) CN108350447B (https=)
AU (1) AU2016303026B2 (https=)
BR (1) BR112018002395A2 (https=)
CA (1) CA2992170C (https=)
DK (1) DK3332005T3 (https=)
ES (1) ES2902044T3 (https=)
IL (1) IL257000B (https=)
TW (1) TW201718859A (https=)
WO (1) WO2017021541A1 (https=)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI560272B (en) 2011-10-07 2016-12-01 Lonza Ag Regulatable promoter
JP7717459B2 (ja) 2018-02-12 2025-08-04 ロンザ リミテッド 目的タンパク質を産生するための宿主細胞
EP3536784A1 (en) 2018-03-05 2019-09-11 ACIB GmbH Host cell engineered for improved metabolite production
EP3802832A1 (en) * 2018-06-07 2021-04-14 Basf Se Promoter for yeast
EP3824083A4 (en) * 2018-09-24 2022-01-05 Orta Dogu Teknik Universitesi Design of alcohol dehydrogenase 2 (adh2) promoter variants by promoter engineering
JP2022502018A (ja) * 2018-09-24 2022-01-11 オルタ ドグ テクニク ユニヴェルシテシ 改変されたａｏｘ１プロモータバリアント
DK3874047T4 (da) * 2019-01-11 2025-02-03 Lonza Ag Karbonkilde-reguleret proteinproduktion i en rekombinant værtscelle
RS64248B1 (sr) * 2019-01-29 2023-06-30 Clariant Produkte Deutschland Gmbh Himerni promotor za upotrebu u metaboličkom inženjeringu
AU2020252241B2 (en) 2019-04-01 2024-03-21 Universität Für Bodenkultur Wien Mut- methylotrophic yeast
WO2020200414A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Protein production in mut-methylotrophic yeast
WO2020200415A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Mut- methylotrophic yeast
US11618900B2 (en) * 2019-11-01 2023-04-04 The Johns Hopkins University Modulating SYNGAP
EP4127185B1 (en) 2020-04-01 2025-06-04 Lonza Ltd. Helper factors for expressing proteins in yeast
CA3174666A1 (en) * 2020-04-09 2021-10-14 Robert Schlegl Improved methods of producing a lipidated protein
CA3193155A1 (en) 2020-09-30 2022-04-07 Lonza Ltd Host cells overexpressing translational factors
IL304910A (en) 2021-02-12 2023-10-01 Boehringer Ingelheim Rcv Gmbh Signal peptides for increased protein secretion
CN114657190B (zh) * 2022-04-06 2023-08-29 暨南大学 Msn2p作为负调控因子在提高宿主细胞中蛋白表达中的应用
CN114908092B (zh) * 2022-05-12 2023-07-18 华南理工大学 一种长效、高强度的杂合启动子及其重组菌株和应用
EP4353825A1 (en) * 2022-10-10 2024-04-17 bisy GmbH Modified promoter sequences
WO2024133538A1 (en) 2022-12-20 2024-06-27 Lonza Ltd Host cells with pat1 knockout for increased specific protein productivity
EP4726051A1 (en) * 2023-06-06 2026-04-15 FUJIFILM Corporation Recombinant yeast, method for producing target protein, and kit and method for producing recombinant yeast
CN121358867A (zh) * 2023-06-15 2026-01-16 丹斯塔发酵股份公司 C2碳源响应性启动子
WO2025247966A1 (en) 2024-05-29 2025-12-04 Mybios Gmbh Process for protein production in methylotrophic yeast host cell.
WO2026027251A1 (en) 2024-08-01 2026-02-05 Boehringer Ingelheim Rcv Gmbh & Co Kg Signal peptides for increased protein secretion
BE1032887B1 (nl) * 2024-08-26 2026-04-14 Anheuser Busch Inbev Sa Geoptimaliseerd promotorontwerp voor industriëe toepassing

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090325241A1 (en) * 2008-06-13 2009-12-31 Thomas William Jeffries Sugar transport sequences, yeast strains having improved sugar uptake, and methods of use
WO2014067926A1 (en) * 2012-10-29 2014-05-08 Lonza Ltd Expression sequences
CN103998460A (zh) * 2011-10-07 2014-08-20 隆扎有限公司 可调控启动子

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT501955B1 (de) 2005-02-23 2007-08-15 Univ Graz Tech Mutierte aox1-promotoren
EP2494053A4 (en) * 2009-10-30 2013-10-30 Merck Sharp & Dohme PROCESS FOR PRODUCING RECOMBINANT PROTEINS WITH INCREASED SECRETION EFFICIENCY

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090325241A1 (en) * 2008-06-13 2009-12-31 Thomas William Jeffries Sugar transport sequences, yeast strains having improved sugar uptake, and methods of use
CN103998460A (zh) * 2011-10-07 2014-08-20 隆扎有限公司 可调控启动子
WO2014067926A1 (en) * 2012-10-29 2014-05-08 Lonza Ltd Expression sequences

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Pichia pastoris:protein production host and model organism for biomedical research;Brigitte Gasser等;《Future Microbiology》;20130228;第8卷(第2期);第191-208页 *

Also Published As

Publication number Publication date
WO2017021541A1 (en) 2017-02-09
DK3332005T3 (da) 2022-01-03
JP6833812B2 (ja) 2021-02-24
CA2992170C (en) 2022-08-16
HK1251256A1 (zh) 2019-01-25
EP3332005A1 (en) 2018-06-13
CA2992170A1 (en) 2017-02-09
JP2018522565A (ja) 2018-08-16
US12522837B2 (en) 2026-01-13
BR112018002395A2 (pt) 2018-09-18
KR20180037237A (ko) 2018-04-11
US20200347391A1 (en) 2020-11-05
AU2016303026B2 (en) 2020-10-22
TW201718859A (zh) 2017-06-01
IL257000A (en) 2018-03-29
CN108350447A (zh) 2018-07-31
EP3332005B1 (en) 2021-09-22
AU2016303026A1 (en) 2018-02-15
US10752907B2 (en) 2020-08-25
KR102253479B1 (ko) 2021-05-21
US20180223293A1 (en) 2018-08-09
ES2902044T3 (es) 2022-03-24
IL257000B (en) 2022-06-01

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