BR112018002395A2 - variantes de promotor, ácido nucleico do promotor operacionalmente ligado a uma sequência de sinal e uma sequência que codifica uma proteína,construção de expressão, célula hospedeira quecompreende tal construção de expressão, e métodode produção de uma poi - Google Patents
variantes de promotor, ácido nucleico do promotor operacionalmente ligado a uma sequência de sinal e uma sequência que codifica uma proteína,construção de expressão, célula hospedeira quecompreende tal construção de expressão, e métodode produção de uma poiInfo
- Publication number
- BR112018002395A2 BR112018002395A2 BR112018002395A BR112018002395A BR112018002395A2 BR 112018002395 A2 BR112018002395 A2 BR 112018002395A2 BR 112018002395 A BR112018002395 A BR 112018002395A BR 112018002395 A BR112018002395 A BR 112018002395A BR 112018002395 A2 BR112018002395 A2 BR 112018002395A2
- Authority
- BR
- Brazil
- Prior art keywords
- promoter
- seq
- expression construct
- sequence
- region
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP2015068024 | 2015-08-05 | ||
| EP16163932 | 2016-04-05 | ||
| PCT/EP2016/068784 WO2017021541A1 (en) | 2015-08-05 | 2016-08-05 | Promoter variants |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112018002395A2 true BR112018002395A2 (pt) | 2018-09-18 |
Family
ID=56684642
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112018002395A BR112018002395A2 (pt) | 2015-08-05 | 2016-08-05 | variantes de promotor, ácido nucleico do promotor operacionalmente ligado a uma sequência de sinal e uma sequência que codifica uma proteína,construção de expressão, célula hospedeira quecompreende tal construção de expressão, e métodode produção de uma poi |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US10752907B2 (https=) |
| EP (1) | EP3332005B1 (https=) |
| JP (1) | JP6833812B2 (https=) |
| KR (1) | KR102253479B1 (https=) |
| CN (1) | CN108350447B (https=) |
| AU (1) | AU2016303026B2 (https=) |
| BR (1) | BR112018002395A2 (https=) |
| CA (1) | CA2992170C (https=) |
| DK (1) | DK3332005T3 (https=) |
| ES (1) | ES2902044T3 (https=) |
| IL (1) | IL257000B (https=) |
| TW (1) | TW201718859A (https=) |
| WO (1) | WO2017021541A1 (https=) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI560272B (en) | 2011-10-07 | 2016-12-01 | Lonza Ag | Regulatable promoter |
| JP7717459B2 (ja) | 2018-02-12 | 2025-08-04 | ロンザ リミテッド | 目的タンパク質を産生するための宿主細胞 |
| EP3536784A1 (en) | 2018-03-05 | 2019-09-11 | ACIB GmbH | Host cell engineered for improved metabolite production |
| EP3802832A1 (en) * | 2018-06-07 | 2021-04-14 | Basf Se | Promoter for yeast |
| EP3824083A4 (en) * | 2018-09-24 | 2022-01-05 | Orta Dogu Teknik Universitesi | Design of alcohol dehydrogenase 2 (adh2) promoter variants by promoter engineering |
| JP2022502018A (ja) * | 2018-09-24 | 2022-01-11 | オルタ ドグ テクニク ユニヴェルシテシ | 改変されたａｏｘ１プロモータバリアント |
| DK3874047T4 (da) * | 2019-01-11 | 2025-02-03 | Lonza Ag | Karbonkilde-reguleret proteinproduktion i en rekombinant værtscelle |
| RS64248B1 (sr) * | 2019-01-29 | 2023-06-30 | Clariant Produkte Deutschland Gmbh | Himerni promotor za upotrebu u metaboličkom inženjeringu |
| AU2020252241B2 (en) | 2019-04-01 | 2024-03-21 | Universität Für Bodenkultur Wien | Mut- methylotrophic yeast |
| WO2020200414A1 (en) | 2019-04-01 | 2020-10-08 | Lonza Ltd | Protein production in mut-methylotrophic yeast |
| WO2020200415A1 (en) | 2019-04-01 | 2020-10-08 | Lonza Ltd | Mut- methylotrophic yeast |
| US11618900B2 (en) * | 2019-11-01 | 2023-04-04 | The Johns Hopkins University | Modulating SYNGAP |
| EP4127185B1 (en) | 2020-04-01 | 2025-06-04 | Lonza Ltd. | Helper factors for expressing proteins in yeast |
| CA3174666A1 (en) * | 2020-04-09 | 2021-10-14 | Robert Schlegl | Improved methods of producing a lipidated protein |
| CA3193155A1 (en) | 2020-09-30 | 2022-04-07 | Lonza Ltd | Host cells overexpressing translational factors |
| IL304910A (en) | 2021-02-12 | 2023-10-01 | Boehringer Ingelheim Rcv Gmbh | Signal peptides for increased protein secretion |
| CN114657190B (zh) * | 2022-04-06 | 2023-08-29 | 暨南大学 | Msn2p作为负调控因子在提高宿主细胞中蛋白表达中的应用 |
| CN114908092B (zh) * | 2022-05-12 | 2023-07-18 | 华南理工大学 | 一种长效、高强度的杂合启动子及其重组菌株和应用 |
| EP4353825A1 (en) * | 2022-10-10 | 2024-04-17 | bisy GmbH | Modified promoter sequences |
| WO2024133538A1 (en) | 2022-12-20 | 2024-06-27 | Lonza Ltd | Host cells with pat1 knockout for increased specific protein productivity |
| EP4726051A1 (en) * | 2023-06-06 | 2026-04-15 | FUJIFILM Corporation | Recombinant yeast, method for producing target protein, and kit and method for producing recombinant yeast |
| CN121358867A (zh) * | 2023-06-15 | 2026-01-16 | 丹斯塔发酵股份公司 | C2碳源响应性启动子 |
| WO2025247966A1 (en) | 2024-05-29 | 2025-12-04 | Mybios Gmbh | Process for protein production in methylotrophic yeast host cell. |
| WO2026027251A1 (en) | 2024-08-01 | 2026-02-05 | Boehringer Ingelheim Rcv Gmbh & Co Kg | Signal peptides for increased protein secretion |
| BE1032887B1 (nl) * | 2024-08-26 | 2026-04-14 | Anheuser Busch Inbev Sa | Geoptimaliseerd promotorontwerp voor industriëe toepassing |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AT501955B1 (de) | 2005-02-23 | 2007-08-15 | Univ Graz Tech | Mutierte aox1-promotoren |
| US8105811B2 (en) | 2008-06-13 | 2012-01-31 | Wisconsin Alumni Research Foundation | Sugar transport sequences, yeast strains having improved sugar uptake, and methods of use |
| EP2494053A4 (en) * | 2009-10-30 | 2013-10-30 | Merck Sharp & Dohme | PROCESS FOR PRODUCING RECOMBINANT PROTEINS WITH INCREASED SECRETION EFFICIENCY |
| TWI560272B (en) * | 2011-10-07 | 2016-12-01 | Lonza Ag | Regulatable promoter |
| AU2013341049B2 (en) | 2012-10-29 | 2017-07-20 | Lonza Ltd | Expression sequences |
-
2016
- 2016-08-05 DK DK16751266.4T patent/DK3332005T3/da active
- 2016-08-05 AU AU2016303026A patent/AU2016303026B2/en active Active
- 2016-08-05 KR KR1020187006217A patent/KR102253479B1/ko active Active
- 2016-08-05 WO PCT/EP2016/068784 patent/WO2017021541A1/en not_active Ceased
- 2016-08-05 BR BR112018002395A patent/BR112018002395A2/pt not_active IP Right Cessation
- 2016-08-05 JP JP2018506219A patent/JP6833812B2/ja active Active
- 2016-08-05 ES ES16751266T patent/ES2902044T3/es active Active
- 2016-08-05 TW TW105125044A patent/TW201718859A/zh unknown
- 2016-08-05 US US15/750,334 patent/US10752907B2/en active Active
- 2016-08-05 EP EP16751266.4A patent/EP3332005B1/en active Active
- 2016-08-05 CA CA2992170A patent/CA2992170C/en active Active
- 2016-08-05 CN CN201680058388.8A patent/CN108350447B/zh active Active
-
2018
- 2018-01-18 IL IL257000A patent/IL257000B/en unknown
-
2020
- 2020-06-29 US US16/914,866 patent/US12522837B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| WO2017021541A1 (en) | 2017-02-09 |
| DK3332005T3 (da) | 2022-01-03 |
| JP6833812B2 (ja) | 2021-02-24 |
| CA2992170C (en) | 2022-08-16 |
| HK1251256A1 (zh) | 2019-01-25 |
| EP3332005A1 (en) | 2018-06-13 |
| CA2992170A1 (en) | 2017-02-09 |
| JP2018522565A (ja) | 2018-08-16 |
| US12522837B2 (en) | 2026-01-13 |
| CN108350447B (zh) | 2021-08-31 |
| KR20180037237A (ko) | 2018-04-11 |
| US20200347391A1 (en) | 2020-11-05 |
| AU2016303026B2 (en) | 2020-10-22 |
| TW201718859A (zh) | 2017-06-01 |
| IL257000A (en) | 2018-03-29 |
| CN108350447A (zh) | 2018-07-31 |
| EP3332005B1 (en) | 2021-09-22 |
| AU2016303026A1 (en) | 2018-02-15 |
| US10752907B2 (en) | 2020-08-25 |
| KR102253479B1 (ko) | 2021-05-21 |
| US20180223293A1 (en) | 2018-08-09 |
| ES2902044T3 (es) | 2022-03-24 |
| IL257000B (en) | 2022-06-01 |
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Legal Events
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| B07D | Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette] |
Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NO 10196/2001, QUE MODIFICOU A LEI NO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUENCIA PREVIA DA ANVISA. CONSIDERANDO A APROVACAO DOS TERMOS DO PARECER NO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDENCIAS CABIVEIS. |
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| B07E | Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette] | ||
| B06U | Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette] | ||
| B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 6A ANUIDADE. |
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| B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2682 DE 31-05-2022 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013. |