CN108342427A - A kind of method and purposes improving uncooked amylum digestion rate - Google Patents
A kind of method and purposes improving uncooked amylum digestion rate Download PDFInfo
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- CN108342427A CN108342427A CN201810077828.5A CN201810077828A CN108342427A CN 108342427 A CN108342427 A CN 108342427A CN 201810077828 A CN201810077828 A CN 201810077828A CN 108342427 A CN108342427 A CN 108342427A
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- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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Abstract
The present invention provides a kind of method and purposes improving uncooked amylum digestion rate, and the preparation method includes：A, starch is uniformly mixed with Protein Extraction Reagent, obtains suspension；B, suspension is stirred；C, stratum granulosum is taken out into the suspension centrifugation after stirring；D, stratum granulosum is cleaned and is filtered, obtain filter cake；E, by filtration cakes torrefaction；Up to the uncooked amylum of high digestion rate.Product of the present invention by low power consuming low cost in a manner of be efficiently peeled off starch granules binding protein (SGAPs), maintain original starch structure integrality and physicochemical property stability on the basis of for the first time find stripping SGAPs after starch be more easy to by Amylase Hydrolysis.The purposes includes being used as industrial production raw material, and the production efficiency of starch sugar can be promoted in sugar industry, reduces energy consumption.
Description
Technical field
The invention belongs to field of food, and in particular to a kind of method and purposes improving uncooked amylum digestion rate.
Background technology
Starch is one of maximum natural polymer of molecular weight, is the main matter of plant storage energy, simultaneously
It is also the substrate of amylorrhexis reaction.According to the rate for discharging glucose after enzymolysis, starch can be divided into (20 points of rapid digestion starch
Decompose in clock), slowly digestible starch (being decomposed in 20-120 minute) and resistant starch (not decomposed in 120 minutes), this method lead to
It is usually used in describing the complexity of amylorrhexis.Meanwhile starch is as being widely present in cereal, root, stem and the fruit of plant
Glucose high polymer, be most important raw materials for production in sugar industry.
Starch sugar is the major product of starch deep processing, remarkable effect is played in food industry economy, but at this stage
There are still technical bottlenecks in starch sugar production process.Traditional handicraft is to use thermophilic digestion enzymatic isolation method again, and thermophilic digestion makes
Starch occurs to be gelatinized this process, need to consume a large amount of energy, and generate a large amount of industrial wastewater.In recent years, by alphalise starch
Enzyme and glucuroide compound action in starch sugar production process, directly by raw material starch liquefacation, saccharification " two enzymes method ",
Environmentally friendly and energy saving aspect has more apparent advantage than traditional boiling.And how more efficiently raw material Starch Production to be utilized to form sediment
Pulverized sugar is always the research hotspot of industry concern, but the enzymolysis mechanism of starch granules is not fully apparent from yet.
Most protein are effectively removed during starch isolation, but starch granules binding protein (starch
Granule-associated proteins, SGAPs) division rejecting can not be scraped by the protein in starch isolation.Although forming sediment
SGAPs contents in powder are only 0.8% or so, but these micro protein and grain quality are closely related.SGAPs is generally same
When be present in starch particle surface and inner passage, be known respectively as starch particle surface albumen (starch granule
Channel proteins) and starch granules channel protein (starch granule surface proteins).In SGAPs
Hydrophobic grouping and starch granules produce strong interaction, general starch extracting method can not be effectively peeled off.
Relationship between SGAPs and amylorrhexis ability is rarely reported.
Invention content
For the defects in the prior art, the object of the present invention is to provide it is a kind of improve uncooked amylum digestion rate method and
Purposes is specifically to provide a kind of starch, the starch easily digest it is easy to digest, can be used as the raw material of industry, be used in sugar industry
Starch sugar product is produced more efficiently.
The purpose of the present invention is what is be achieved through the following technical solutions：
The present invention provides a kind of methods improving uncooked amylum digestion rate, and described method includes following steps：
A, starch is uniformly mixed with Protein Extraction Reagent, obtains suspension；
B, suspension is stirred；
C, stratum granulosum is taken out into the suspension centrifugation after stirring；
D, stratum granulosum is cleaned and is filtered, obtain filter cake；
E, by filtration cakes torrefaction；
Up to the uncooked amylum of high digestion rate.
Preferably, in step A, the Starches include in rice starch, cornstarch, wheaten starch, potato starch
At least one；The mass volume ratio of the starch and Protein Extraction Reagent is 1:2~1:5；The Protein Extraction Reagent includes
The each component of following concentration or volumn concentration：25~50mM trishydroxymethylaminomethanes (pH=7~8), 5~20% the third three
Alcohol, 0.05~2% sodium dodecyl sulfate solution and 0.1~4% mercaptoethanol.The sodium dodecyl sulfate solution and mercapto
Base ethyl alcohol is to protein from being removed on starch and have facilitation in being soluble in the aqueous phase.
It is highly preferred that the Protein Extraction Reagent includes each component of following concentration or volumn concentration：25~50mM
Trishydroxymethylaminomethane (pH=7~8), 10~15% glycerine, 1~2% sodium dodecyl sulfate solution and 1~2% mercapto
Base ethyl alcohol.Each component is matched in the range, and the peeling effect of the SGAPs in starch can be made best, the enzymolysis energy of starch
Power is best.The sodium dodecyl sulfate solution too high levels are by indissoluble solution；And mercaptoethanol too high levels will be toxic and different
Taste, it is unfavorable for later-period purification starch, ideal starch product can not be obtained.
Preferably, in step B, the agitating mode is 200~400 revs/min and at the uniform velocity stirs；The mixing time is 36
~72 hours.The bigger extraction efficiency of stir speed (S.S.) is higher, but rate too conference blistering is unfavorable for subsequent operation and limit effect
It should successively decrease, therefore 200~400 revs/min are most suitable mixing speed.
Preferably, in step C, the centrifugation step is specially：By suspension with 4000~8000 revs/min centrifugation 10~
20 minutes, supernatant liquor is poured out, digs out the stratum granulosum of lower layer.
Preferably, in step D, the cleaning is specially with step is filtered：Starch is placed in 80% ethyl alcohol and is uniformly mixed,
Decompression filters, and collects filter cake；With absolute ethyl alcohol dissolving filter cake and filter again, be repeated up to filtrate clarification, in filter cake no liquid after
It is continuous to filter out.
Preferably, in step E, the drying condition is that 40 DEG C of baking ovens are dried 48~72 hours.
The present invention also provides a kind of farinose purposes based on high digestion rate made from preceding method, the purposes
Include the raw material as sugar industry, for producing starch sugar.
The method of the present invention has been effectively peeled off SGAPs, has been obviously improved the enzymolysis ability of starch, can more rapidly decompose
With release glucose, it can be used as the raw material of industry, for starch sugar product to be produced more efficiently in sugar industry.It is limited to pass
System technology, relevant product are also more rare on the market, it is contemplated that have certain market prospects.
Compared with prior art, the present invention has following advantageous effect：
(1) the method for the present invention has been effectively peeled off the SGAPs in starch, finds that the content for reducing SGAPs can be carried significantly for the first time
The enzymolysis ability of high starch；
(2) the method for the present invention is easy to implement, production efficiency is high, production cost is low, energy consumption is low, is starch sugar in sugar industry
Production provide raw material basis.
Description of the drawings
Upon reading the detailed description of non-limiting embodiments with reference to the following drawings, other feature of the invention,
Objects and advantages will become more apparent upon：
Fig. 1 is the rapid digestion content of starch variation effect of the front and back starch of SGAPs strippings.
Specific implementation mode
With reference to specific embodiment, the present invention is described in detail.Following embodiment will be helpful to the technology of this field
Personnel further understand the present invention, but the invention is not limited in any way.It should be pointed out that the ordinary skill of this field
For personnel, without departing from the inventive concept of the premise, several changes and improvements can also be made.These belong to the present invention
Protection domain.
Embodiment 1
The present embodiment is related to a kind of preparation method of starch, and described method includes following steps：
Step 1,70 grams of general rice starch (amylose content 14%) are weighed in 200 milliliters of Protein Extraction Reagents (to contain
50mM trishydroxymethylaminomethanes (pH=8), 10% glycerine, 0.1% sodium dodecyl sulfate solution and 0.2% sulfydryl second
Alcohol) in, it is uniformly mixed；
Step 2, magneton is used at the uniform velocity to be stirred 48 hours with 300 revs/min after mixing；
Step 3, suspension is poured into centrifuge tube and is centrifuged 15 minutes with 6000 revs/min, poured out supernatant liquor, dig out down
The stratum granulosum of layer；
Step 4, starch is placed in 500 milliliter of 80% ethyl alcohol and is uniformly mixed, and is depressurized and is filtered with Buchner funnel, collects filter
Cake.It with 1000 milliliters of absolute ethyl alcohol dissolving filter cakes and filters again, is repeated up to filtrate clarification, no liquid continues to filter out in filter cake；
Step 5, filter cake is dried 48 hours in 40 DEG C of baking ovens, is ground up, sieved to get starch granules binding protein has been removed
Starch.
Implementation result：SGAPs types in the supernatant liquor that the present embodiment is isolated by LS-MS/MS authentication steps 3, it is main
To be 9 kinds of starch granules channel proteins such as granule bound starch synzyme (Q0DEV5) and rare starch particle surface albumen, BCA
Residual protein was 0.39% (ative starch contains 0.54%) in the starch that method determination step 5 obtains, and illustrated that the present embodiment can be effective
Starch granules channel protein is removed, but starch particle surface albumen cannot be effectively peeled off.The rapid digestion content of starch of ative starch
It is 36.1%, the rapid digestion content of starch of the present embodiment starch is 35.4%, and there was no significant difference.
Embodiment 2
The present embodiment is related to a kind of preparation method of starch, and the method is same as Example 1, the difference is that only：
In step 1, with Waxy Rice Starch (amylose content 0.1%)；
Implementation result：The present embodiment identifies SGAPs types, predominantly Q-enzyrne in supernatant liquor by LS-MS/MS
(A2X5K0) etc. 3 kinds of starch granules channel proteins and rare starch particle surface albumen, BCA methods measure residual protein in starch
For 0.53% (ative starch contains 0.77%), illustrate that the present embodiment can be effectively peeled off starch granules channel protein, but cannot effectively shell
From starch particle surface albumen.The rapid digestion content of starch of ative starch is 51.0%, and the rapid digestion of the present embodiment starch forms sediment
Powder content is 47.0%, and rapid digestion content of starch declines.
Embodiment 3
The present embodiment is related to a kind of preparation method of starch, and the method is same as Example 1, the difference is that only：
In step 1, Protein Extraction Reagent (trishydroxymethylaminomethane containing 50mM (pH=8), 10% glycerine, 1.5% ten
Dialkyl sulfonates solution and 2% mercaptoethanol)；
Implementation result：The present embodiment identifies SGAPs types in supernatant liquor by LS-MS/MS, exists simultaneously particle combination
16 kinds of starch particle surface eggs such as 9 kinds of starch granules channel proteins such as amylosynthease (Q0DEV5) and glutelin (Q84X94)
In vain, it was only 0.12% (ative starch contains 0.54%) that BCA methods, which measure residual protein in starch, illustrated that the present embodiment can simultaneously effectively
Remove starch granules channel protein and starch particle surface albumen.The rapid digestion content of starch of ative starch is 36.1%, this reality
The rapid digestion content of starch for applying starch is 46.0%, and rapid digestion content of starch significantly rises.
Embodiment 4
The present embodiment is related to a kind of preparation method of starch, and the method is same as Example 1, the difference is that only：
In step 1, with Waxy Rice Starch (amylose content 0.1%)；
In step 1, Protein Extraction Reagent (trishydroxymethylaminomethane containing 50mM (pH=8), 10% glycerine, 1.5% ten
Dialkyl sulfonates solution and 2% mercaptoethanol)；
Implementation result：The present embodiment identifies SGAPs types in supernatant liquor by LS-MS/MS, exists simultaneously starch branch
11 kinds of starch particle surface albumen such as 3 kinds of starch granules channel proteins such as enzyme (A2X5K0) and glutelin (Q84X94), BCA methods are surveyed
It was only 0.10% (ative starch contains 0.77%) to determine residual protein in starch, illustrated that the present embodiment can be effectively peeled off starch simultaneously
Grain channel protein and starch particle surface albumen.The rapid digestion content of starch of ative starch is 51.0%, the present embodiment starch
Rapid digestion content of starch is 54.3%, and rapid digestion content of starch rises.
Embodiment 3 is most highly preferred embodiment of the invention, effectively can remove starch granules channel protein and starch granules simultaneously
Surface protein, rapid digestion starch are promoted to 46% by 36.1%, and growth rate 27.4% is the maximum in all embodiments
Value.Present invention firstly discovers that stripping only starch granules channel protein can reduce rapid digestion content of starch (Examples 1 and 2), and
Rapid digestion content of starch (embodiment can be obviously improved by removing starch granules channel protein and starch particle surface albumen but simultaneously
3 and 4).The technology of the present invention has low cost, low power consuming and easy-operating feature, and effectively prevents in traditional technology to starch
Structural damage ensures that its stability in following process application, the production for starch sugar in sugar industry provide raw material
Basis.
The starch and its corresponding ative starch that embodiment 1-4 is prepared are all made of Englyst methods and determine rapid digestion
Starch (in 20 minutes by Amylase Hydrolysis be glucose starch) content, the results are shown in Figure 1.
Embodiment 5
The present embodiment is related to a kind of preparation method of starch, the described method comprises the following steps：
Step 1,70 grams of general rice starch (amylose content 14%) are weighed in 140 milliliters of Protein Extraction Reagents (to contain
25mM trishydroxymethylaminomethanes (pH=8), 10% glycerine, 1% dodecyl sodium sulfonate solution and 1% mercaptoethanol) in, it mixes
It closes uniform；
Step 2, magneton is used at the uniform velocity to be stirred 72 hours with 200 revs/min after mixing；
Step 3, suspension is poured into centrifuge tube and is centrifuged 10 minutes with 8000 revs/min, poured out supernatant liquor, dig out down
The stratum granulosum of layer；
Step 4, starch is placed in 1000 milliliter of 80% ethyl alcohol and is uniformly mixed, and is depressurized and is filtered with Buchner funnel, collects filter
Cake.It with 2000 milliliters of absolute ethyl alcohol dissolving filter cakes and filters again, is repeated up to filtrate clarification, no liquid continues to filter out in filter cake；
Step 5, filter cake is dried 72 hours in 40 DEG C of baking ovens, is ground up, sieved to get starch granules binding protein has been removed
Starch.
Implementation result：The present embodiment identifies SGAPs types in supernatant liquor by LS-MS/MS, exists simultaneously particle combination
16 kinds of starch particle surface eggs such as 9 kinds of starch granules channel proteins such as amylosynthease (Q0DEV5) and glutelin (Q84X94)
In vain, it was 0.31% (ative starch contains 0.54%) that BCA methods, which measure residual protein in starch, illustrated that the present embodiment can while effectively be shelled
From starch granules channel protein and starch particle surface albumen.The rapid digestion content of starch of ative starch is 36.1%, this implementation
The rapid digestion content of starch of example starch is 41.1%, and rapid digestion content of starch rises.
Embodiment 6
The present embodiment is related to a kind of preparation method of starch, the described method comprises the following steps：
Step 1,70 grams of Waxy Rice Starchs (amylose content 0.1%) are weighed in 350 milliliters of Protein Extraction Reagents (to contain
40mM trishydroxymethylaminomethanes (pH=8), 15% glycerine, 2% dodecyl sodium sulfonate solution and 1.5% mercaptoethanol) in,
It is uniformly mixed；
Step 2, magneton is used at the uniform velocity to be stirred 36 hours with 400 revs/min after mixing；
Step 3, suspension is poured into centrifuge tube and is centrifuged 20 minutes with 4000 revs/min, poured out supernatant liquor, dig out down
The stratum granulosum of layer；
Step 4, starch is placed in 2000 milliliter of 80% ethyl alcohol and is uniformly mixed, and is depressurized and is filtered with Buchner funnel, collects filter
Cake.It with 500 milliliters of absolute ethyl alcohol dissolving filter cakes and filters again, is repeated up to filtrate clarification, no liquid continues to filter out in filter cake；
Step 5, filter cake is dried 60 hours in 40 DEG C of baking ovens, is ground up, sieved to get starch granules binding protein has been removed
Starch.
The present embodiment identifies SGAPs types in supernatant liquor by LS-MS/MS, exists simultaneously Q-enzyrne
(A2X5K0) etc. 11 kinds of starch particle surface albumen such as 3 kinds of starch granules channel proteins and glutelin (Q84X94), BCA methods measure
Residual protein was only 0.19% (ative starch contains 0.77%) in starch, illustrated that the present embodiment can be effectively peeled off starch granules simultaneously
Channel protein and starch particle surface albumen.The rapid digestion content of starch of ative starch be 51.0%, the present embodiment starch it is fast
Fast digestible starch content is 52.4%, and rapid digestion content of starch rises.
Comparative example 1
This comparative example is related to a kind of preparation method of starch, and the method is same as Example 3, the difference is that only：
In step 1, Protein Extraction Reagent (trishydroxymethylaminomethane containing 50mM (pH=8), 10% glycerine, 1.5% ten
Dialkyl sulfonates solution)；
Implementation result：The present embodiment identifies SGAPs types in supernatant liquor by LS-MS/MS, and there is only particles to combine shallow lake
1 kind of starch granules channel protein such as powder synzyme (Q0DEV5), is not present starch particle surface albumen, and BCA methods measure in starch
Residual protein is 0.51% (ative starch contain 0.54%), illustrate the present embodiment cannot be effectively peeled off starch granules channel protein and
Starch particle surface albumen.The rapid digestion content of starch of ative starch is 36.1%, the rapid digestion starch of the present embodiment starch
Content is 36.0%, and rapid digestion content of starch is without significant change.
Comparative example 2
This comparative example is related to a kind of preparation method of starch, and the method is same as Example 3, the difference is that only：
In step 1, Protein Extraction Reagent (trishydroxymethylaminomethane containing 50mM (pH=8), 10% glycerine, 2% sulfydryl
Ethyl alcohol)；
Implementation result：The present embodiment identifies SGAPs types in supernatant liquor by LS-MS/MS, and there is only Q-enzyrnes
(A2X5K0) etc. a kind of starch granules channel protein, is not present starch particle surface albumen, and BCA methods measure residual protein in starch
Matter was 0.72% (ative starch contains 0.77%), illustrated that the present embodiment cannot be effectively peeled off starch granules channel protein and starch granules
Surface protein.The rapid digestion content of starch of ative starch is 51.0%, and the rapid digestion content of starch of the present embodiment starch is
50.7%, rapid digestion content of starch is without significant change.
Specific embodiments of the present invention are described above.It is to be appreciated that the invention is not limited in above-mentioned
Particular implementation, those skilled in the art can make a variety of changes or change within the scope of the claims, this not shadow
Ring the substantive content of the present invention.In the absence of conflict, the feature in embodiments herein and embodiment can arbitrary phase
Mutually combination.
Claims (7)
1. a kind of method improving uncooked amylum digestion rate, which is characterized in that described method includes following steps：
A, starch is uniformly mixed with Protein Extraction Reagent, obtains suspension；
B, suspension is stirred；
C, stratum granulosum is taken out into the suspension centrifugation after stirring；
D, stratum granulosum is cleaned and is filtered, obtain filter cake；
E, by filtration cakes torrefaction；
Up to the uncooked amylum of high digestion rate.
2. starch preparation method according to claim 1, which is characterized in that in step A, the Starches include rice
At least one of starch, cornstarch, wheaten starch, potato starch；The quality volume of the starch and Protein Extraction Reagent
Than being 1:2~1:5；The Protein Extraction Reagent includes each component of following concentration or volumn concentration：25~50mM, tri- hydroxyls
Aminomethane, 5~20% glycerine, 0.05~2% sodium dodecyl sulfate solution and 0.1~4% mercaptoethanol.
3. starch preparation method according to claim 1, which is characterized in that in step B, the agitating mode be 200~
400 revs/min are at the uniform velocity stirred；The mixing time is 36~72 hours.
4. starch preparation method according to claim 1, which is characterized in that in step C, the centrifugation step is specially：
Suspension is centrifuged 10~20 minutes with 4000~8000 revs/min, supernatant liquor is poured out, digs out the stratum granulosum of lower layer.
5. starch preparation method according to claim 1, which is characterized in that in step D, the cleaning has with step is filtered
Body is：Starch is placed in 80% ethyl alcohol and is uniformly mixed, and decompression filters, and collects filter cake；It with absolute ethyl alcohol dissolving filter cake and takes out again
Filter is repeated up to filtrate clarification, and no liquid continues to filter out in filter cake.
6. starch preparation method according to claim 1, which is characterized in that in step E, the drying condition is 40 DEG C of bakings
Case is dried 48~72 hours.
7. a kind of farinose purposes based on high digestion rate made from claim 1 the method, which is characterized in that described
Purposes includes the raw material as sugar industry, for producing starch sugar.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115232219A (en) * | 2022-08-19 | 2022-10-25 | 上海交通大学 | Preparation method of deproteinized toughened modified starch |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105622768A (en) * | 2015-12-21 | 2016-06-01 | 上海交通大学 | Soybean starch extracting method |
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2018
- 2018-01-26 CN CN201810077828.5A patent/CN108342427B/en active Active
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105622768A (en) * | 2015-12-21 | 2016-06-01 | 上海交通大学 | Soybean starch extracting method |
Non-Patent Citations (2)
| Title |
|---|
| KAREN V.PINEDA-HIDALGO等: "Physicochemical，Structural，and Proteomic Analysis of Starch Granules from Maize Landraces of Northwest Mexico", 《CEREAL CHEMISTRY》 * |
| PAUL M.BALDWIN: "Starch Granule-Associated Proteins and Polypeptides：A Review", 《STARCH STÄRKE》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115232219A (en) * | 2022-08-19 | 2022-10-25 | 上海交通大学 | Preparation method of deproteinized toughened modified starch |
| CN115232219B (en) * | 2022-08-19 | 2023-08-22 | 上海交通大学 | Preparation method of deproteinized and toughened modified starch |
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