CN108338068B - Method for transforming and improving rape cytoplasmic male sterile line - Google Patents

Abstract

The invention discloses a method for transforming and improving rape cytoplasmic male sterile line (CMS), which comprises the following steps: hybridizing hybrid seeds prepared from rape cytoplasmic male sterile lines as male parents and a cytoplasmic male sterile maintainer line to be transferred as a female parent to obtain an F1 generation; planting F1 generation, selecting single plant with excellent agronomic character for emasculation, and backcrossing with the male parent as recurrent parent to obtain BCF1 generation; planting BCF1 generations, and bagging and selfing single plants with excellent agronomic characters to obtain BCF2 generations; planting 100 plants in BCF2 generation, selecting individual plants with excellent agronomic characters from BCF2 generation segregating population for selfing, simultaneously carrying out test crossing with stable cytoplasmic male sterile line, carrying out test crossing for 5-10 plants, harvesting seeds after the selfing individual plants are mature for the next generation to continue to carry out the selective selfing, carrying out backcross with the individual plants which are thoroughly sterile and have excellent agronomic characters in the test crossing F1 generation, carrying out the continuous selective selfing to BCF7 generation, and carrying out the backcross of the sterile individual plants to BC5F1 generation to obtain the final product.

Description

Method for transforming and improving rape cytoplasmic male sterile line

Technical Field

The invention relates to the field of rape breeding, in particular to a method for transforming and improving rape cytoplasmic male sterile line (CMS).

Background

The rape belongs to Brassica (Brassica) plants in Cruciferae, is one of four oil crops in the world, is also a main oil crop in China, and plays an important role in national economy and people's life in China. The rape heterosis is obvious, and the yield can be increased by 20-30% generally, so the utilization of the rape heterosis is one of the important fields of rape breeding. The method for utilizing the rape heterosis in the world is most common in a quality sterile system, the basis for breeding quality sterile hybrid rape varieties is the breeding of three-line materials, and the yield potential and the production application area of the hybrid rape varieties are directly influenced by the stability of the sterility of the quality sterile line and the breeding level.

Disclosure of Invention

In order to solve the above problems, the present invention provides a method for transforming and improving cytoplasmic male sterile line (CMS) of rape.

In order to achieve the purpose, the invention adopts the technical scheme that:

a method for transforming and improving rape cytoplasmic male sterile line (CMS) comprises the following steps:

s1, hybridizing the hybrid seeds prepared by rape cytoplasmic male sterile line (CMS) as male parent and the cytoplasmic male sterile maintainer line to be transferred as female parent to obtain F1 generation;

s1, planting F1 generations, selecting single plants with excellent agronomic characters for emasculation, and backcrossing by using the male parents as recurrent parents to obtain BCF1 generations;

s3, planting BCF1 generations, and bagging and selfing single plants with excellent agronomic characters to obtain BCF2 generations;

s4, planting 100 plants of BCF2, selecting single plants with excellent agronomic characters from a BCF2 segregating population for selfing, simultaneously carrying out test crossing with a stable cytoplasmic male sterile line, carrying out test crossing on 5-10 plants, harvesting seeds after the selfing single plants are mature for the next generation to continue to carry out selfing, backcrossing with the single plants which are thoroughly sterile and have excellent agronomic characters in a test crossing F1 generation, carrying out continuous selection selfing to BCF7 generation, and backcrossing the sterile single plants to BC5F1 generation, thus a stable cytoplasmic male sterile line (CMS) of the rape and corresponding maintainer line materials can be selectively bred.

The invention has the following beneficial effects:

improves some bad characters of the existing rape homozygous cytoplasmic male sterile line (CMS), and enhances the excellent quality and the high yield performance of the rape homozygous cytoplasmic male sterile line.

Drawings

FIG. 1 is a schematic flow chart of a method for transforming modified rape cytoplasmic male sterile line (CMS) according to the present invention.

Detailed Description

In order that the objects and advantages of the invention will be more clearly understood, the invention is further described in detail below with reference to examples. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

As shown in FIG. 1, the embodiment of the present invention provides a method for transforming modified rape cytoplasmic male sterile line (CMS), comprising the following steps:

s1, taking the hybrid seed prepared by rape cytoplasmic male sterile line (CMS) as a male parent, taking the castrated improved cytoplasmic male sterile maintainer line as a female parent, and hybridizing to obtain hybrid seed F1;

s2, planting 50 plants of each generation of the male parent in the step S1 and the hybrid seeds F1 harvested on the female parent, selecting 5-10 plants of F1 generation with excellent agronomic characters, disease resistance and lodging resistance in the flowering period, emasculating, continuously using the hybrid seeds prepared by a cytoplasmic male sterile line (CMS) as the male parent for pollination, and harvesting backcross seeds BCF1 generation seeds;

s3, planting harvested BCF1 seeds, selecting 10-20 single plants with excellent agronomic characters, disease resistance and lodging resistance in the flowering period, bagging, selfing, and harvesting BCF2 seeds after maturation;

s4, planting stable rape cytoplasmic male sterile line seeds and the obtained BCF2 generation seeds by a plant division line, selecting 10-20 individual plants of a BCF2 generation line in a flowering period, bagging and selfing, taking pollen of the seeds and the stable rape cytoplasmic male sterile line for test cross, and harvesting BCF3 generation seeds and test cross seed F1 generation seeds after the seeds are mature;

s5, planting BCF3 generation seeds and corresponding test cross species F1 generation seeds in a plant division line, selecting a single plant which is completely sterile in the test cross species F1 generation in a flowering period to backcross with the corresponding BCF3, bagging and selfing BCF3, and harvesting BCF4 seeds and BC1F1 seeds after maturation; continuously selecting and selfing BCF4 to BCF8 generations through 4 generations, and backcrossing the corresponding sterile line BC1F1 to BC5F1 generations;

s6, breeding the harvested BCF8 generation seeds in an isolation net room, planting BCF8 and BC5F1 seeds in another isolation net room according to the parent-parent row ratio of 2: 4, and carrying out seed production in the net room of the sterile line BC5F1 seeds, wherein the harvested seeds are the newly transferred and improved rape cytoplasmic sterile line and the corresponding maintainer line.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that those skilled in the art can make various improvements and modifications without departing from the principle of the present invention, and these improvements and modifications should also be construed as the protection scope of the present invention.

Claims (1)

1. A method for transforming and improving rape cytoplasmic male sterile line is characterized by comprising the following steps:

s1, hybridizing the hybrid seeds prepared by rape cytoplasmic male sterile line as male parent and the cytoplasmic male sterile maintainer line to be transferred as female parent to obtain F1 generation;

s1, planting F1 generations, selecting single plants with excellent agronomic characters for emasculation, and backcrossing by using the male parents as recurrent parents to obtain BCF1 generations;

s3, planting BCF1 generations, and bagging and selfing single plants with excellent agronomic characters to obtain BCF2 generations;

s4, planting stable rape cytoplasmic male sterile line seeds and the obtained BCF2 generation seeds by a plant division line, selecting 10-20 individual plants of a BCF2 generation line in a flowering period, bagging and selfing, taking pollen of the seeds and the stable rape cytoplasmic male sterile line for test cross, and harvesting BCF3 generation seeds and test cross seed F1 generation seeds after the seeds are mature;

s5, planting BCF3 generation seeds and corresponding test cross species F1 generation seeds in a plant division line, selecting a single plant which is completely sterile in the test cross species F1 generation in a flowering period to backcross with the corresponding BCF3, bagging and selfing BCF3, and harvesting BCF4 seeds and BC1F1 seeds after maturation; continuously selecting and selfing BCF4 to BCF8 generations through 4 generations, and backcrossing the corresponding sterile line BC1F1 to BC5F1 generations;

s6, breeding the harvested BCF8 generation seeds in an isolation net room, planting BCF8 and BC5F1 seeds in another isolation net room according to the parent-parent row ratio of 2: 4, and carrying out seed production in the net room of the sterile line BC5F1 seeds, wherein the harvested seeds are the newly transferred and improved rape cytoplasmic sterile line and the corresponding maintainer line.

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