CN108308457A - A kind of composite feed additive and the preparation method and application thereof improving jewfish liver mitochondrion function - Google Patents
A kind of composite feed additive and the preparation method and application thereof improving jewfish liver mitochondrion function Download PDFInfo
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- CN108308457A CN108308457A CN201810147841.3A CN201810147841A CN108308457A CN 108308457 A CN108308457 A CN 108308457A CN 201810147841 A CN201810147841 A CN 201810147841A CN 108308457 A CN108308457 A CN 108308457A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/116—Heterocyclic compounds
- A23K20/137—Heterocyclic compounds containing two hetero atoms, of which at least one is nitrogen
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/19—Carboxylic acids, e.g. valproic acid
- A61K31/195—Carboxylic acids, e.g. valproic acid having an amino group
- A61K31/197—Carboxylic acids, e.g. valproic acid having an amino group the amino and the carboxyl groups being attached to the same acyclic carbon chain, e.g. gamma-aminobutyric acid [GABA], beta-alanine, epsilon-aminocaproic acid, pantothenic acid
- A61K31/198—Alpha-aminoacids, e.g. alanine, edetic acids [EDTA]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
- A61K31/4353—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom ortho- or peri-condensed with heterocyclic ring systems
- A61K31/4375—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom ortho- or peri-condensed with heterocyclic ring systems the heterocyclic ring system containing a six-membered ring having nitrogen as a ring heteroatom, e.g. quinolizines, naphthyridines, berberine, vincamine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/24—Heavy metals; Compounds thereof
- A61K33/32—Manganese; Compounds thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/24—Heavy metals; Compounds thereof
- A61K33/34—Copper; Compounds thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/062—Ascomycota
- A61K36/064—Saccharomycetales, e.g. baker's yeast
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Abstract
The present invention relates to a kind of composite feed additives and the preparation method and application thereof improving jewfish liver mitochondrion function, belong to field of fodder.The composite feed additive of every 100 parts by weight include the methionine chelate manganese of 6 10 parts by weight, the Methionine chelate copper of 26 parts by weight, the berberine of 30 50 parts by weight, the creatine of 20 30 parts by weight and 0.5 2 parts by weight red phaffia rhodozyma, surplus is carrier.The raw material of the composite feed additive is safe, without hazard residue, and condition is created for production green feed and aquatic products.Preparation method is：Above-mentioned raw materials are mixed according to the ratio.This preparation method is simple, and condition controllability is good, and stability is good, has a good application prospect.Above-mentioned composite feed additive is applied to prepare jewfish feed, the oxidation resistance and mitochondrial respiration-chain function of jewfish liver mitochondrion can be improved, reduce Mitochondrial oxidative damage.
Description
Technical field
The present invention relates to field of fodder, and more particularly to a kind of composite feed improving jewfish liver mitochondrion function adds
Add agent and the preparation method and application thereof.
Background technology
In recent years, with high density, intensive, industrialized aquiculture development, the frequent hair of jewfish fatty liver is cultivated
Life is the Universal Problems that aquaculture faces.Fatty liver not only results in the jewfish speed of growth and declines with feed conversion rate, can also
The immunity for reducing jewfish brings huge economic losses to jewfish cultivation, seriously affects the sound development of jewfish cultivation.According to me
Laboratory result of study for many years, find the dysfunction of mitochondria be jewfish fatty liver occur basic reason.Mitochondria
The reduction of fatty acid decomposition energy, fat is caused to be easy in jewfish liver over-deposit after dysfunction.In addition, mitochondrial function
Obstacle also results in body and generates oxidative stress.Energy supply is necessarily affected after mitochondria dysfunction, then immunity of organism
Reaction is affected.Therefore, phenomena such as jewfish fatty liver often occurs oxidative damage, hypoimmunity, all hinders with mitochondrial function
Hindering has close relationship.
Therefore, the product that exploitation can improve jewfish liver mitochondrion function has very important effect.
Invention content
The purpose of the present invention is to provide a kind of composite feed additives improving jewfish liver mitochondrion function, this is multiple
The raw material of mould assembly feed addictive is safe, without hazard residue, and condition is created for production green feed and aquatic products.
The second object of the present invention is to provide a kind of preparation method of above-mentioned feed addictive, and the preparation method is simple,
Condition controllability is good, and stability is good, has a good application prospect.
The third object of the present invention is to provide application of the above-mentioned feed addictive in jewfish feed, is applied to system
Standby jewfish feed, can be improved the oxidation resistance and mitochondrial respiration-chain function of jewfish liver mitochondrion, reduce mitochondria oxygen
Change damage.
The present invention solves its technical problem using following technical scheme to realize：
The present invention proposes a kind of composite feed additive improving jewfish liver mitochondrion function, every 100 parts by weight
Composite feed additive includes the methionine chelate manganese of 6-10 parts by weight, the Methionine chelate copper of 2-6 parts by weight, 30-50 weights
The berberine, the creatine of 20-30 parts by weight and the red phaffia rhodozyma of 0.5-2 parts by weight of part are measured, surplus is carrier.
The present invention also proposes the preparation method of above-mentioned composite feed additive：Mixing methionine Chelated Manganese, egg according to the ratio
Propylhomoserin chelated copper, berberine, creatine, red phaffia rhodozyma and carrier.
In addition, the application the invention also provides above-mentioned composite feed additive in preparing jewfish feed, such as can
It is made an addition in jewfish feed for the ratio of 0.5-1.5% by weight percent.
The composite feed additive and its system for the improvement jewfish liver mitochondrion function that present pre-ferred embodiments provide
Preparation Method and the advantageous effect of application are：
(1) raw material selected by is safe, without hazard residue, and condition is created for production green feed and aquatic products.
(2) product target for modulation is clear, safe and efficient, formula is reasonable, has synergistic effect, compatible use ratio between each component
Single use effect is more preferable, more stable.
(3) activity of the respiratory chain complex of mitochondria can be improved in product, promotes energetic supersession, the splicing thread grain of mitochondria
The generation ability of body ATP.
(4) product can improve mitochondria generative capacity, promote the regeneration of mitochondria.
(5) preparation method is simple, and condition controllability is good, and feed stability is good, has good application prospect.
(6) it is used to prepare jewfish feed and feeds jewfish, the oxidation resistance and line of jewfish liver mitochondrion can be improved
Mitochondrial respiratory chain function reduces Mitochondrial oxidative damage.
Description of the drawings
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technology description to be briefly described.
Fig. 1 is normally to organize structure chart of the jewfish liver cell under Electronic Speculum in test example 2；
Fig. 2 is structure chart of the high in fat group of jewfish liver cell under Electronic Speculum in test example 2；
Fig. 3 is structure chart of the 3 jewfish liver cell of test group under Electronic Speculum in test example 2.
Specific implementation mode
It in order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below will be in the embodiment of the present invention
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, builds according to normal condition or manufacturer
The condition of view carries out.Reagents or instruments used without specified manufacturer is the conventional production that can be obtained by commercially available purchase
Product.
Composite feed additive to the improvement jewfish liver mitochondrion function of the embodiment of the present invention and its preparation below
Method is specifically described with application.
It has been investigated that the dysfunction of mitochondria is the basic reason that fatty,fiss liver occurs.Mitochondria is aliphatic acid
The main place of beta oxidation, the also source of active oxygen generation.Mitochondria dysfunction not only results in the reduction of fatty acid decomposition energy,
Fat is easily in liver over-deposit.Moreover, mitochondria dysfunction, which also results in reactive oxygen species, generates raising, body is made to produce
Raw oxidative stress.Mitochondria is the center of energetic supersession, and energy supply is necessarily affected after dysfunction, and energy deficiency is also
Can organism immune response be affected.
Therefore, phenomena such as fatty,fiss liver often occurs oxidative damage, hypoimmunity all with the dysfunction of mitochondria
There is extremely close relationship.
Based on above-mentioned discovery, in the embodiment of the present invention, the composite feed additive of every 100 parts by weight for example may include
The methionine chelate manganese of 6-10 parts by weight, the Methionine chelate copper of 2-6 parts by weight, the berberine of 30-50 parts by weight, 20-30 weights
The creatine of part and the red phaffia rhodozyma of 0.5-2 parts by weight are measured, surplus is carrier.
Further, the above-mentioned composite feed additive of every 100 parts by weight for example may include the egg ammonia of 7-9 parts by weight
Sour Chelated Manganese, the Methionine chelate copper of 3-5 parts by weight, the berberine of 35-45 parts by weight, 23-27 parts by weight creatine and
The red phaffia rhodozyma of 0.5-1.5 parts by weight, surplus are carrier.
Further, the above-mentioned composite feed additive of every 100 parts by weight for example may include the egg ammonia of 8 parts by weight
Sour Chelated Manganese, the Methionine chelate copper of 4 parts by weight, the berberine of 40 parts by weight, the creatine of 25 parts by weight and 1 parts by weight it is red
Phaffia rhodozyma, surplus are carrier.
Optionally, above-mentioned carrier includes defatted rice bran and/or powdered rice hulls.
Wherein, methionine chelate manganese primarily serves in the composite feed additive that the present invention program provides provides manganese member
The effect of element.Constituent or activator of the manganese as a variety of enzymes, especially as the important set of manganese peroxidase mutase
At ingredient, the peroxide of mitochondria can be eliminated, protection mitochondria is from oxidative damage.
Secondly, also contain methionine in methionine chelate manganese, lipid peroxide damage primary and secondary can be avoided molten
Enzyme body film, the acid phosphatase as hydrolysis for causing to contain in lysosome release and important to cell and mitochondrial membrane etc.
Organelle damage.In addition, methionine can also promote liver plasma membrane phospholipid methylation, membrane fluidity is made to enhance, reduced
The siltation of bile in liver cell, reinforcement turn sulfenyl effect, reduce bile acid and are built up in liver, have preferable detoxication.
By using the methionine chelate manganese simultaneously containing methionine and manganese, the work of methionine and manganese can not only be had both
With, and can also more individual methionine or manganese there is higher biological value, not only reduce the dosage of manganese, also reduce
Production cost.
Preferably, the ligand ratio (molar ratio of methionine and manganese element) in above-mentioned methionine chelate manganese is preferably controlled in
2.5-3：1, this ligand is relatively suitble to jewfish to be absorbed and utilized than the stability of methionine chelate manganese in range.Ligand can be caused than too small
The chelate of formation is unstable, and free manganese ion, which can be catalyzed, causes hydroxyl radical free radical to generate；Ligand is than excessive, though chelate is steady
Qualitative enhancing, but after its stability is too strong, be absorbed and utilized not only bad for jewfish, while can also cause the waste of methionine, from
And increase production cost.
Methionine chelate copper mainly plays anti-oxidation function in the feed addictive of the embodiment of the present invention, can remove freedom
Base protects the integrality of mitochondrial membrane.It provides methionine for jewfish jointly with methionine chelate manganese, and protects mitochondria normal
Running.
Preferably, the copper in the embodiment of the present invention contained by Methionine chelate copper is Cu2+.Preferably, above-mentioned methionine chelate
The stability of copper is stronger preferably at 92-93%, this chelation percent for the chelation percent of copper in copper, not only can avoid in jewfish body
It is catalyzed hydroxyl radical free radical to generate, while being also well suited to digesting and assimilating for jewfish.
Further, the ligand ratio (molar ratio of methionine and copper) of Methionine chelate copper is excellent in the embodiment of the present invention
Selected control system is in 2-2.5：1, this ligand is than the methionine chelate manganese with above-mentioned chelation percent (92-93%) in range relatively steady
While determining, also it is absorbed and utilized compared with conducive to jewfish.
Berberine is also referred to as jamaicin, and berberine is commonly used in the treatment disease of digestive tracts such as bacillary enteritis or dysentery at present,
Separately also there is application in terms of adjusting intestinal flora and constipation.But in the embodiment of the present invention, by regarding berberine as material composition
One of, the key factor of its active cell mitochondria biogenesis can be utilized, the biosynthesis of mitochondria, the oxygen of aliphatic acid are promoted
Change, while also there is certain anti-oxidation function.
Creatine is naturally occurring amino acid derivativges, primarily serve in embodiments of the present invention for cell supplement energy,
The effects that enhancing cell biological energy, enhance the function of mitochondria and reducing the apoptosis that mitochondria mediates.
Red phaffia rhodozyma can enhance the activity of jewfish mitochondrial respiratory chain Complex II, reduce line in embodiments of the present invention
The improper autophagy of plastochondria and division, promote the generation of mitochondria, and phaffiafhodozyma also has strong anti-oxidative activity.
Above-mentioned raw materials ingredient also has synergistic function, example other than respectively having individual effect between each raw material
Such as：Methionine chelate manganese has the oxidative damage for cooperateing with antioxidation and capable of reducing mitochondria with Methionine chelate copper.Egg
Propylhomoserin Chelated Manganese also has with berberine cooperates with antioxidation, also, methionine chelate manganese can enhance berberine and promote line grain
The effect that body generates.Methionine chelate manganese can cooperate with the energetic supersession ability for improving mitochondria with creatine, supplement cellular energy.
Methionine chelate manganese can cooperate with anti-oxidant with red phaffia rhodozyma, improve the activity of mitochondrial respiratory chain.Berberine can promote
Mitochondria generates the expression of gene, and creatine provides energy for mitochondria generation, and the two collaboration improves the generation of mitochondria.
From the above, after the raw material in the feed addictive of the embodiment of the present invention is used cooperatively, liver can be effectively improved
The function of mitochondria improves the fatty acid decomposition ability of mitochondria, alleviates the generation of fatty liver.
In addition, the embodiment of the present invention additionally provides a kind of preparation method of above-mentioned composite feed additive, namely by upper
State proportioning mixing methionine Chelated Manganese, Methionine chelate copper, berberine, creatine, red phaffia rhodozyma and carrier.
Optionally, since the content of red phaffia rhodozyma, Methionine chelate copper and methionine chelate manganese is less, especially
Red phaffia rhodozyma, uneven to avoid mixing, such as causing certain parts, certain parts are free of red Fife containing phaffiafhodozyma
Carrier can be divided into two parts in the embodiment of the present invention, first by red phaffia rhodozyma, methionine chelate manganese, egg ammonia by the case where yeast
Sour chelated copper is uniformly mixed with first part carrier, is then mixed again with berberine, creatine by formula ratio, finally again with it is remaining
Second part carrier mixes, to so that each component is uniformly mixed.
Preferably, before mixing, solid material can be crushed to 150-180 μm, it is uniform with the particle for improving solid material
Property, improve the edibility and digestibility of feed addictive.
In addition, the embodiment of the present invention additionally provides a kind of application of above-mentioned composite feed additive, such as can be used
In preparing jewfish feed and feeding jewfish, so as to the energy for effectively improving jewfish liver mitochondrion quantity, improving mitochondria
Metabolic capability and respiratory chain activity and raising mitochondrial antioxidant ability, improve jewfish liver mitochondrion function to reach
Effect.
Optionally, which for example can be that 0.5-1.5% makes an addition to sea by weight percent
It in perch feed, such as can be added in jewfish feed by 0.5wt%, 1wt% and 1.5wt%, be preferably added to jewfish by 1wt%
In feed.Under the adding proportion, the cost performance of feed addictive is best.
The feature and performance of the present invention are described in further detail with reference to embodiments.
Embodiment 1
Contain the methionine chelate manganese of 6 parts by weight, the egg of 2 parts by weight by the composite feed additive of every 100 parts by weight
The red phaffia rhodozyma of propylhomoserin chelated copper, the berberine of 30 parts by weight, the creatine of 20 parts by weight and 0.5 parts by weight, surplus are de-
The ratio of fat rice bran mixes each raw material, must improve the composite feed additive of jewfish liver mitochondrion function.
Wherein, the ligand ratio of methionine chelate manganese is 2.5：1.Copper contained by Methionine chelate copper is Cu2+, methionine chelate
The chelation percent of copper is 92% in copper, and the ligand ratio of Methionine chelate copper is 2：1.
Embodiment 2
The present embodiment and embodiment 1 difference lies in：Contain 10 weight by the composite feed additive of every 100 parts by weight
The methionine chelate manganese of part, the creatine and 2 weights of the Methionine chelate copper of 6 parts by weight, the berberine of 50 parts by weight, 30 parts by weight
The red phaffia rhodozyma of part is measured, surplus is that the ratio of defatted rice bran mixes each raw material, must improve jewfish liver mitochondrion work(
The composite feed additive of energy.
Embodiment 3
The present embodiment and embodiment 1 difference lies in：Contain 7 weight by the composite feed additive of every 100 parts by weight
The methionine chelate manganese of part, the creatine and 0.5 of the Methionine chelate copper of 3 parts by weight, the berberine of 35 parts by weight, 23 parts by weight
The red phaffia rhodozyma of parts by weight, surplus are that the ratio of defatted rice bran mixes each raw material, must improve jewfish liver mitochondrion
The composite feed additive of function.
Embodiment 4
The present embodiment and embodiment 1 difference lies in：Contain 9 weight by the composite feed additive of every 100 parts by weight
The methionine chelate manganese of part, the creatine and 1.5 of the Methionine chelate copper of 5 parts by weight, the berberine of 45 parts by weight, 27 parts by weight
The red phaffia rhodozyma of parts by weight, surplus are that the ratio of defatted rice bran mixes each raw material, must improve jewfish liver mitochondrion
The composite feed additive of function.
Embodiment 5
The present embodiment and embodiment 1 difference lies in：Contain 8 weight by the composite feed additive of every 100 parts by weight
The methionine chelate manganese of part, the creatine and 1 weight of the Methionine chelate copper of 4 parts by weight, the berberine of 40 parts by weight, 25 parts by weight
The red phaffia rhodozyma of part is measured, surplus is that the ratio of defatted rice bran mixes each raw material, must improve jewfish liver mitochondrion work(
The composite feed additive of energy.
Embodiment 6
The present embodiment and embodiment 1 difference lies in：Ligand ratio in methionine chelate manganese is 3：1.
Embodiment 7
The present embodiment and embodiment 1 difference lies in：Ligand ratio in methionine chelate manganese is 2.75：1.
Embodiment 8
The present embodiment and embodiment 1 difference lies in：The chelation percent of copper is 93% in Methionine chelate copper.
Embodiment 9
The present embodiment and embodiment 1 difference lies in：The chelation percent of copper is 92.5% in Methionine chelate copper.
Embodiment 10
The present embodiment and embodiment 1 difference lies in：Carrier is powdered rice hulls.
Embodiment 11
The present embodiment and embodiment 1 difference lies in：Carrier is the mixture of defatted rice bran and powdered rice hulls.
Embodiment 12
The present embodiment and embodiment 1 difference lies in：The ligand ratio of Methionine chelate copper is 2.5：1.
Embodiment 13
The present embodiment and embodiment 1 difference lies in：The ligand ratio of Methionine chelate copper is 2.25：1.
Embodiment 14
The present embodiment and embodiment 1 difference lies in：In mixed process, carrier is divided into two parts, it according to the ratio first will be red
Phaffia rhodozyma, methionine chelate manganese, Methionine chelate copper and first part carrier are uniformly mixed, and then press formula ratio and the coptis again
Element, creatine mixing, finally mix with remaining second part carrier again.
Embodiment 15
The present embodiment provides a kind of applications for the composite feed additive improving jewfish liver mitochondrion function, are pressed
Mass percent makes an addition to for 0.5% in jewfish feed.
Embodiment 16
The present embodiment provides a kind of applications for the composite feed additive improving jewfish liver mitochondrion function, are pressed
Mass percent makes an addition to for 1% in jewfish feed.
Embodiment 17
The present embodiment provides a kind of applications for the composite feed additive improving jewfish liver mitochondrion function, are pressed
Mass percent makes an addition to for 1.5% in jewfish feed.
Test example
It repeats to implement above-described embodiment 1-14, obtains the composite feed of enough improvement jewfish liver mitochondrion functions
Additive.
It as test group 1-3 by taking embodiment 1-2 and embodiment 5 as an example, is designed using single-factor, test fish is tamed one
Zhou Hou selects 1500 tail of jewfish of physically fit, specification uniform (counterpoise is 200 ± 3g), be randomly divided into 5 groups it is (control group, high in fat
Group, test group 1-3), every group of 3 repetitions are each to repeat 100 tails.Jewfish cultivate in 15 seawater cages (specification be 2.0m ×
2.0m × 2.0m) in cultivation.During culture experiment, feed daily 2 times (7：30 and 17：30) it, is satiated with food and feeds, the culture-cycle 10
Week.Every group of feed formula is as shown in table 1, normal group feed fat content 11%, high in fat group：Feed fat level is 20% (this
Laboratory early-stage study shows：20% fat meeting liver injury mitochondria), test group：Feed fat level is 20%, and is added
This additive.It is machined to the particle that grain size is 5mm or so with small particle, room temperature, which air-dries, to be placed on 4 DEG C of refrigerators and save backup.
Culture experiment terminates, and fasting is for 24 hours.Count the mantissa of fish in each net cage.By the fish MS- of a concentration of 100mg/L
222 (Sigma, the U.S.) anaesthetize, and are placed on ice pan and acquire liver, and extract mitochondria, for testing following index.Test result
As shown in table 2 to table 5.
1 each group feed formula group % of table
Note：Vitamin premix (mg or g/kg feeds) contains thiamine 25mg, riboflavin 45mg, hydrochloric acid respectively in table 1
Pyridoxol 20mg, vitamin B120.1mg, vitamin K310mg, inositol 800mg, pantothenic acid 60mg, niacin 200mg, folic acid 20mg,
Biotin 1.2mg, retinyl acetate 32mg, vitamin D35mg, alpha-tocopherol 120mg, ethoxyquinoline 150mg, choline
(50%) 5000mg.
Mineral substance premix (mg/kg feeds) contains magnesium sulfate monohydrate 4000mg, manganese sulfate monohydrate 50mg, potassium iodide respectively
(1%) 100mg, cobalt chloride (1%) 100mg, cupric sulfate pentahydrate 20mg, ferrous sulfate monohydrate 260mg, monohydrate zinc sulphate 150mg,
Sodium selenite (1%) 50mg.
Influence of the 2 different tests group of table to jewfish liver mitochondrion content
Note：Colleague's subscript does not have same letter to indicate significant difference (P<0.05).
As can be seen from Table 2, after the composite feed additive that addition embodiment 1,2,5 is provided, the jewfish of test group
Mitochondrial protein content be significantly higher than high in fat group of (P<0.05), with normal group without significant difference (P>0.05).Test group and height
Fat group is compared, and mitochondria DNA copy number and mitochondria density are significantly increased (P<0.05), with normal group without significant difference.Table
Bright addition composite feed additive provided in an embodiment of the present invention can be improved jewfish mitochondrial protein content, DNA copy number with
And mitochondria density.
The index of 3 different tests group mitochondrial function of table
Note：Colleague's subscript does not have same letter to indicate significant difference (P<0.05).
As can be seen from Table 3, after the composite feed additive that addition embodiment 1,2,5 is provided, test group jewfish
Mitochondrial isocitrate dehydrogenase, succinate dehydrogenase and Na+,K+- ATPase activity significantly increases (P compared with to high in fat group<
0.05), show that composite feed additive provided in an embodiment of the present invention has enhancing mitochondria without significant difference with normal group
The effect of function.In addition, after the composite feed additive that addition embodiment 1,2,5 is provided, the aliphatic acid of test group jewfish
More high in fat group of decomposition rate significantly improves, and the indifference compared with normal group shows composite feed provided in an embodiment of the present invention
The fatty acid decomposition ability of mitochondria can be improved in additive.
4 different tests group mitochondrial respiratory chain complex activity of table
Note：Colleague's subscript does not have same letter to indicate significant difference (P<0.05).
As can be seen from Table 4, after the composite feed additive that addition embodiment 1,2,5 is provided, test group jewfish
Mitochondrial respiratory chain composite I, more high in fat group of II, III are able to significantly improve (P<0.05), and reach normal group of level, table
Bright composite feed additive provided in an embodiment of the present invention can improve the respiratory chain activity of mitochondria.
5 different tests group Mitochondrial oxidative damage index of table
Note：Colleague's subscript does not have same letter to indicate significant difference (P<0.05).
As can be seen from Table 5, after the composite feed additive that addition embodiment 1,2,5 is provided, test group jewfish
More high in fat group of the total number born SOD activity of mitochondria significantly improves (P<0.05), to enhance anti-oxidation function.And
And after the composite feed additive that is provided of embodiment 1,2,5 is provided, the lipid peroxide MDA of test group jewfish and thin
More high in fat group of considerably lower (P of intracellular ROS contents<0.05), illustrate composite feed additive energy provided in an embodiment of the present invention
Enough oxidative damage degree for effectively reducing mitochondria and being subjected to.
According to the method for above-mentioned test example to the improvement jewfish liver mitochondrion function obtained by 3,4 and 6-14 of embodiment
Composite feed additive is tested, and result equally display contains improvement jewfish provided in an embodiment of the present invention by feeding
The fish meal of the composite feed additive of liver mitochondrion function, can more high in fat group of raising jewfish liver mitochondrion quantity, line
Energetic supersession ability, respiratory chain activity and the oxidation resistance of plastochondria.
Test example 2
By at the end of experimental period in test example 1 test group 3 and the liver cell of normal group and high in fat group of jewfish in electricity
Under the microscope, result is as shown in Figs. 1-3.It can be seen that by Fig. 1-3：The liver cell mitochondrial of normal group jewfish is in pitch black
Color is in slender shape, and mitochondrial matrix is uniform, and interior outer membrane is clear.The mitochondria density of high in fat group of jewfish becomes smaller, intracellular mitochondrial
Number is reduced, and mitochondrial swelling, nucleus collapses, and outer membrane is fuzzy in mitochondria.More high in fat group of test group can increase jewfish line
The quantity of plastochondria alleviates the swelling of mitochondria.Thus illustrate, improvement jewfish liver provided in an embodiment of the present invention is contained by feeding
The jewfish feed of the composite feed additive of mitochondrial function is conducive to maintain the complete normal configuration of jewfish mitochondria.
In conclusion the composite feed additive provided in an embodiment of the present invention for improving jewfish liver mitochondrion function
Raw material is safe, without hazard residue, and condition is created for production green feed and aquatic products.Preparation method is simple, and condition can
Control property is good, and stability is good, has a good application prospect.Above-mentioned composite feed additive is applied to prepare jewfish feed,
The oxidation resistance and mitochondrial respiration-chain function of jewfish liver mitochondrion can be improved, reduce Mitochondrial oxidative damage.
Embodiments described above is a part of the embodiment of the present invention, instead of all the embodiments.The reality of the present invention
The detailed description for applying example is not intended to limit the range of claimed invention, but is merely representative of the selected implementation of the present invention
Example.Based on the embodiments of the present invention, those of ordinary skill in the art are obtained without creative efforts
Every other embodiment, shall fall within the protection scope of the present invention.
Claims (10)
1. a kind of composite feed additive improving jewfish liver mitochondrion function, which is characterized in that the institute of every 100 parts by weight
It includes the methionine chelate manganese of 6-10 parts by weight, the Methionine chelate copper of 2-6 parts by weight, 30-50 to state composite feed additive
The red phaffia rhodozyma of the berberine of parts by weight, the creatine of 20-30 parts by weight and 0.5-2 parts by weight, surplus are carrier.
2. composite feed additive according to claim 1, which is characterized in that every 100 parts by weight it is described compound
Feed addictive includes the methionine chelate manganese of 7-9 parts by weight, the Methionine chelate copper of 3-5 parts by weight, 35-45 weights
Measure the berberine, the creatine of 23-27 parts by weight and the red phaffia rhodozyma of 0.5-1.5 parts by weight of part, surplus
For the carrier.
3. composite feed additive according to claim 2, which is characterized in that every 100 parts by weight it is described compound
Feed addictive includes the methionine chelate manganese of 8 parts by weight, the Methionine chelate copper of 4 parts by weight, 40 parts by weight
The red phaffia rhodozyma of the berberine, the creatine of 25 parts by weight and 1 parts by weight, surplus are the carrier.
4. according to claim 1-3 any one of them composite feed additives, which is characterized in that the methionine chelate manganese
In ligand ratio be 2.5-3：1.
5. according to claim 1-3 any one of them composite feed additives, which is characterized in that the Methionine chelate copper
The chelation percent of middle copper is 92-93%.
6. according to claim 1-3 any one of them composite feed additives, which is characterized in that the carrier includes degreasing
Rice bran and/or powdered rice hulls.
7. the preparation method of composite feed additive as claimed in any one of claims 1 to 6, which is characterized in that mix according to the ratio
Close the methionine chelate manganese, the Methionine chelate copper, the berberine, the creatine, the red phaffia rhodozyma and institute
State carrier.
8. preparation method according to claim 7, which is characterized in that first by the red phaffia rhodozyma, the methionine chela
Conjunction manganese, the Methionine chelate copper are mixed with the part carrier, are then mixed with the berberine, the creatine, finally again
It is mixed with the remaining carrier.
9. application of the composite feed additive as claimed in any one of claims 1 to 6 in preparing jewfish feed, feature
It is, the feed addictive is that the ratio of 0.5-1.5% makes an addition in jewfish feed by weight percent.
10. application according to claim 9, which is characterized in that the feed addictive is 1% ratio by weight percent
Example makes an addition in jewfish feed.
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