CN108157684A - A kind of feed addictive for improving Larimichthys crocea oxidation resistance and preparation method thereof - Google Patents

A kind of feed addictive for improving Larimichthys crocea oxidation resistance and preparation method thereof Download PDF

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Publication number
CN108157684A
CN108157684A CN201810072193.XA CN201810072193A CN108157684A CN 108157684 A CN108157684 A CN 108157684A CN 201810072193 A CN201810072193 A CN 201810072193A CN 108157684 A CN108157684 A CN 108157684A
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parts
resveratrol
feed
fish meal
larimichthys crocea
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李宜聪
艾庆辉
姬仁磊
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Ocean University of China
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/22—Animal feeding-stuffs from material of animal origin from fish
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/105—Aliphatic or alicyclic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/111—Aromatic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/116—Heterocyclic compounds
    • A23K20/132—Heterocyclic compounds containing only one nitrogen as hetero atom
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/30—Oligoelements
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

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Abstract

The present invention provides a kind of feed addictives for improving Larimichthys crocea oxidation resistance, resveratrol are added in rheum officinale fish meal, the additive amount of the resveratrol is between the rheum officinale fish meal and 0.05 0.15wt% of resveratrol gross mass.The preparation method of the feed addictive includes：Resveratrol and rheum officinale fish meal are mixed evenly, you can.The feed addictive of the embodiment of the present invention in rheum officinale fish meal by way of adding a certain amount of resveratrol, to achieve the purpose that improve Larimichthys crocea oxidation resistance, particularly with the high lipid food of raising Larimichthys crocea, provide a kind of functional feedstuff additive that can remarkably promote Larimichthys crocea oxidation resistance, by adjusting feed formula, slow down or even eliminate this because of harm to Larimichthys crocea caused by high lipid food application, advantageous basis has been established in fast but also healthy growth not only for Larimichthys crocea.

Description

A kind of feed addictive for improving Larimichthys crocea oxidation resistance and preparation method thereof
Technical field
The present invention relates to Larimichthys crocea feed processing production field, in particular to a kind of raising anti-oxidant energy of Larimichthys crocea Feed addictive of power and preparation method thereof.
Background technology
Larimichthys crocea (Larimichthys crocea), Osteichthyes, Perciformes (Perciformes), Sciaenidae (Sciaeni-dae), yellow croaker category also known as yellow croaker, great Wang Yu, big fresh, Cymbaria dahurica fish, ivy gourd, Jin Long, gold dragon, sweet osmanthus yellow croaker, Great Zhong, Hong Kou, sciaenoid, stone fish, cucumber fish are one of traditional " four big marine products " (Larimichthys crocea, little yellow croaker, hairtail, cuttlefish), It is China coastal seas Main Commercial Fishes.The Larimichthys crocea of coastal area of china can be divided into 3 populations：1. one East Sea geographical population of East Sea yellow croaker (the first geographical population).Southern Yellow Sea is distributed in Northern Taiwan Strait, including Lv Siyang, Dai Quyang, Sheng mountain fishing ground, cat head Ocean, Dongtou ocean, Fujian official Jing Yang until Minjiang Estuary marine site, be the main foreigner tourists of Chinese rhubarb fish.2. Sound Velocity in Southern Taiwan Strait- East Guangdong geographical population (the second geographical population).3. west of Guangdong Province Yuexi proles (third geographical population).It is distributed mainly on West To Zhujiang Estuary extremely The Nanhai District of Qiongzhou Strait.
Larimichthys crocea is the marine fish of China's cage culture yield maximum, and national total output reaches within 2016 16.55 ten thousand tons.The cultivation of Larimichthys crocea at present is fed still to feed based on chilled fish, but the popularity rate of mixed feed has reached 30%.Especially the system of Larimichthys crocea nutrient research is goed deep into recent years, and Larimichthys crocea special compound feed has been succeeded in developing and has been seen in Market, the use of mixed feed increasingly obtain the approval in market.And chilled fish source is unstable, price fluctuation is big, using not It is convenient, easily corruption grows germ and waste of resources is caused, the problems such as being damaged to environment becomes increasingly conspicuous, mixed feed Demand gradually increase, spreading speed of the mixed feed in rheum officinale fish culture can also be accelerated.
The problem commonly encountered in Larimichthys crocea breeding production is the use of high lipid food, and high lipid food is because of its albumen Matter saves effect and receives extensive attention, but can lead to the damage of Larimichthys crocea oxidation resistance using high lipid food and then lead to feed The generation for the unfavorable situations such as utilization rate reduces, disease is easily sent out, the death rate increases and growth slows down.
In view of this, it is special to propose the present invention.
Invention content
The first object of the present invention is to provide a kind of feed addictive that can reach and improve Larimichthys crocea oxidation resistance, The feed addictive is by way of adding a certain amount of resveratrol in rheum officinale fish meal, is resisted with reaching raising Larimichthys crocea The purpose of oxidability, particularly with the high lipid food of raising Larimichthys crocea, Larimichthys crocea antioxygen can be remarkably promoted by providing one kind The functional feedstuff additive of change ability by adjusting feed formula, slows down or even eliminates this because high lipid food application is brought The harm to Larimichthys crocea, for Larimichthys crocea, advantageous basis has been established in fast but also healthy growth not only.Meanwhile also to improving Larimichthys crocea Series artificial diets important in inhibiting, therefore the feed addictive should be widely promoted application, be also beneficial to create bigger Economic benefit.
The second object of the present invention is to provide the preparation method of above-mentioned feed addictive, preparation method operating procedure letter Single, operating condition is mild, and front and rear step linking is close, and three wastes generate, whole environmentally protective, the feed addictive being prepared Have the Larimichthys crocea oxidation resistance that increases significantly, more feasible way is provided to cultivate out the more excellent Larimichthys crocea of quality Diameter.
In order to realize the above-mentioned purpose of the present invention, spy uses following technical scheme：
The present invention provides a kind of feed addictives for improving Larimichthys crocea oxidation resistance, i.e., are added in rheum officinale fish meal Resveratrol, the additive amount of the resveratrol is the rheum officinale fish meal and the 0.05-0.15wt% of resveratrol gross mass Between.
In the prior art, Larimichthys crocea is the marine fish of China's cage culture yield maximum, national total yield in 2016 Amount has reached 16.55 ten thousand tons.The cultivation of Larimichthys crocea at present is fed still to feed based on chilled fish, but the popularity rate of mixed feed Reach 30%.Especially the system of Larimichthys crocea nutrient research is goed deep into recent years, and Larimichthys crocea special compound feed has been succeeded in developing And market is seen in, the use of mixed feed increasingly obtains the approval in market.And chilled fish source is unstable, price fluctuation is big, Inconvenient to use, easy corruption grows germ and waste of resources is caused, the problems such as being damaged to environment becomes increasingly conspicuous, and matches The demand for closing feed gradually increases, and spreading speed of the mixed feed in rheum officinale fish culture can also be accelerated.
The problem commonly encountered in Larimichthys crocea breeding production is the use of high lipid food, and high lipid food is because of its albumen Matter saves effect and receives extensive attention, but can lead to the damage of Larimichthys crocea oxidation resistance using high lipid food and then lead to feed The generation for the unfavorable situations such as utilization rate reduces, disease is easily sent out, the death rate increases and growth slows down.
The present invention adds in order to solve the above technical problems, providing a kind of feed that can improve Larimichthys crocea oxidation resistance Add agent, which is by adding a certain amount of resveratrol in rheum officinale fish meal, and Larimichthys crocea is improved so as to reach The purpose of oxidation resistance.
Certainly, the additive amount of this resveratrol needs to control in certain range, and additive amount is rheum officinale fish meal With the 0.05-0.15wt% of both resveratrols gross mass.
Preferably, it controls in 0.07-0.10wt%.
In addition to this, the additive amount of resveratrol is the rheum officinale fish meal and the 50-150mg/ of resveratrol gross mass Between kg.
Optimally, it controls between 80-120mg/kg.
In addition to this, the additive amount of resveratrol can also be 90mg/kg, 100mg/kg, 110mg/kg etc., as long as will add Dosage is controlled in range of the presently claimed invention, can reach corresponding effect, may be because if dosage is too small certainly The small effect for not having at all and improving Larimichthys crocea oxidation resistance is measured, if the anti-oxidant energy of the opposite too big Larimichthys crocea of additive amount Power will not be promoted further, thus additive amount need to control it is suitable.Resveratrol under the adding proportion can improve Larimichthys crocea Anti-oxidation stress ability, and apparent side effect is not generated to the liver of Larimichthys crocea.
The purity assay of resveratrol of the present invention is preferably controlled in more than 98% or more than 99%, even Reach 100%, purity height is more advantageous to the efficient performance of its effect.
In addition, the rheum officinale fish meal of the present invention mainly includes：In terms of mass fraction, 20-50 parts of fish meal, 15-20 parts of dregs of beans, 15-20 parts of wheat flour, 1-3 parts of soybean lecithin, 4-7 parts of fish oil, 4-7 parts of soya-bean oil, 1-3 parts of mineral substance premix, phagostimulant 0.2-0.4 parts, 0.05-0.1 parts of ethoxyquin woods, 1-3 parts of vitamin premix.
Preferably, the rheum officinale fish meal mainly includes：In terms of mass fraction, 40 parts of fish meal, 18 parts of dregs of beans, wheat flour 16 Part, 2 parts of soybean lecithin, 6 parts of fish oil, 6 parts of soya-bean oil, 2 parts of mineral substance premix, 0.3 part of phagostimulant, 0.1 part of ethoxyquin woods, 2 parts of vitamin premix.
Above-mentioned feed is that required nutrient is formulated in the breeding process based on Larimichthys crocea, more science Rationally.
The present invention also improves this in addition to providing a kind of formula for the feed addictive for improving Larimichthys crocea oxidation resistance The preparation method of feed addictive, includes the following steps：
Resveratrol and rheum officinale fish meal are mixed evenly, you can.
The preparation method operating procedure of the present invention is simple, and operating condition is mild, and front and rear step linking is close, three wastes production Raw, whole environmentally protective, the feed addictive being prepared has the Larimichthys crocea oxidation resistance that increases significantly, and produces for cultivation The more excellent Larimichthys crocea of matter provides more feasible approach.
Preferably, the temperature of mixing is controlled at 20-30 DEG C.
Preferably, the time control of mixing is in 20-40min.
Preferably, the rate control of mixing is between 200-300rpm.
By the control of each operating parameter in more suitable range, the rheum officinale fish meal of homogeneity can be prepared, Resveratrol is preferably merged into each other with other feed ingredients.
Compared with prior art, beneficial effects of the present invention are：
(1) a kind of feed addictive that can reach and improve Larimichthys crocea oxidation resistance is provided, which is logical The mode that a certain amount of resveratrol is added in rheum officinale fish meal is crossed, to achieve the purpose that improve Larimichthys crocea oxidation resistance, Particularly with the high lipid food of raising Larimichthys crocea, provide a kind of the functional of Larimichthys crocea oxidation resistance that can remarkably promote and raise Feed additives,
(2) present invention is by adjusting feed formula, slow down in addition eliminate this because caused by high lipid food application to rheum officinale The harm of fish, for Larimichthys crocea, advantageous basis has been established in fast but also healthy growth not only.Meanwhile also to improving the cooperation of Larimichthys crocea series Feed important in inhibiting, therefore the feed addictive should be widely promoted application, be also beneficial to create the economic benefit of bigger.
(3) the present invention provides a kind of preparation methods of feed addictive, and the preparation method operating procedure is simple, operate item Part is mild, and front and rear step linking is close, and three wastes generate, and whole environmentally protective, the feed addictive being prepared has notable Raising Larimichthys crocea oxidation resistance, provide more feasible approach to cultivate out the more excellent Larimichthys crocea of quality.
Description of the drawings
It, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical solution of the prior art Embodiment or attached drawing needed to be used in the description of the prior art are briefly described, it should be apparent that, in being described below Attached drawing is some embodiments of the present invention, for those of ordinary skill in the art, before not making the creative labor It puts, can also be obtained according to these attached drawings other attached drawings.
The SOD activity index of the feed addictive of raising Larimichthys crocea oxidation resistance that Fig. 1 is 1-3 of the embodiment of the present invention；
The CAT activity index of the feed addictive of raising Larimichthys crocea oxidation resistance that Fig. 2 is 1-3 of the embodiment of the present invention；
The MDA content's index of the feed addictive of raising Larimichthys crocea oxidation resistance that Fig. 3 is 1-3 of the embodiment of the present invention；
The GSH activity index of the feed addictive of raising Larimichthys crocea oxidation resistance that Fig. 4 is 1-3 of the embodiment of the present invention；
The glutamic-oxalacetic transaminease AST of the feed addictive of raising Larimichthys crocea oxidation resistance that Fig. 5 is 1-3 of the embodiment of the present invention (GOT) content's index.
Specific embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the invention.It is not specified in embodiment specific Condition person, the condition suggested according to normal condition or manufacturer carry out.Reagents or instruments used without specified manufacturer is The conventional products that can be obtained by commercially available purchase.
Embodiment 1-3
Embodiment 1：A kind of rheum officinale fish feed additive, weigh purity assay reach 98% resveratrol preparation with it is high in fat Rheum officinale fish meal other components are according to 0.05：99.95 weight, which is uniformly mixed, is made pellet, and Larimichthys crocea is fed.
Embodiment 2：A kind of rheum officinale fish feed additive, weigh purity assay reach 98% resveratrol preparation with it is high in fat Rheum officinale fish meal other components are according to 0.1：99.9 weight, which is uniformly mixed, is made pellet, and Larimichthys crocea is fed.
Embodiment 3：A kind of rheum officinale fish feed additive, weigh purity assay reach 98% resveratrol preparation with it is high in fat Rheum officinale fish meal other components are according to 0.15：99.85 weight, which is uniformly mixed, is made pellet, and Larimichthys crocea is fed.
Fodder making fat level be 9 Larimichthys crocea pellet as a control group, and Fodder making fat level be 18 Larimichthys crocea pellet as high in fat group, specific dispensing see the table below 1：
The preparation method of each group feed carries out in accordance with the following steps：Resveratrol and rheum officinale fish meal other compositions are being stirred It mixes in container and is mixed evenly, 20 DEG C, mixing time 20min of temperature control, stir speed (S.S.) is controlled in 300rpm.
Table 1 tests feed formula
Wherein mineral substance premix (mg/kg)：Epsom salt (MgSO4.7H2O), 180；Cupric sulfate pentahydrate (CuSO4.5H2O), 2.5；Ferrous sulfate monohydrate (FeSO4.H2O), 24；Monohydrate zinc sulphate (ZnSO4.H2O), 17.25；One water sulphur Sour manganese (MnSO4.H2O), 14.31；Cobalt chloride (CoCl2), 0.5；Sodium selenite (Na2SeO3), 0.2；Calcium iodate (Ca (IO3) 2.6H2O), 0.6；10000 microcrystalline cellulose of calcium dihydrogen phosphate, 8485.But common mineral in rheum officinale fish meal can also be selected Matter mixture；
Vitamin premix (mg/kg)：VA Acetate, 16000IU；D3 particles, 2500IU；Alpha-tocopherol, 120；Sulfurous Sour hydrogen sodium menadione (MSB), 5.1；Thiamine hydrochloride, 24.5；Riboflavin particle, 36；Pyridoxol, 19.8；Cyanocobalamin, 0.1；D- Calcium pantothenate, 58.8；Niacin, 198；Folic acid, 19.6；Biotin, 1.2；Inositol, 784；Choline chloride, 2000.City can also be used The vitamin mixtures of other aquatic products fields sold.
Embodiment 4
Other operating procedures are consistent with embodiment 3, and only resveratrol preparation is pressed with rheum officinale fish meal other components high in fat According to 0.06：99.994 weight is uniformly mixed, in preparation method, 30 DEG C, mixing time 40min of temperature control, and stir speed (S.S.) control In 200rpm.
Embodiment 5
Other operating procedures are consistent with embodiment 3, and only resveratrol preparation is pressed with rheum officinale fish meal other components high in fat According to 0.07：99.993 weight is uniformly mixed, in preparation method, 25 DEG C, mixing time 30min of temperature control, and stir speed (S.S.) control In 250rpm.
Embodiment 6-8
Other operating procedures are consistent with embodiment 3, and only feed formula carries out dispensing according to such as the following table 2：
Table 2 tests feed formula
Experimental example 1
Feed addictive is made in composition of raw materials in table 1 and is added to feeding Larimichthys crocea 10 weeks in rheum officinale fish meal, is measured Liver resistance to oxidation index.The same a collection of Larimichthys crocea of current year artificial incubation is selected as experiment fish, average weight for 13.75 ± 0.27g.The Larimichthys crocea that specification is consistent, physically fit is picked out to put in a suitable place to breed in the seawater floating net that 15 specifications are 1m × 1m × 1.5m In case, 60 tails are put in each net cage in a suitable place to breed.3 net cages of each feedstuff feeding, culture experiment carry out 70 days altogether.After culture experiment Carry out analysis experiment.
Glutamic-oxalacetic transaminease is primarily present in liver cell mitochondria, when liver occurs severe necrosis or destroys, can just be drawn Play that glutamic-oxalacetic transaminease concentration in serum is higher, if the content of glutamic-oxalacetic transaminease increases severely in serum, may infer that may liver Severe necrosis or destruction occurs, therefore can be used to judge whether liver is damaged by measuring the content of glutamic-oxalacetic transaminease in serum Evil.
Measure five processing group liver Antioxidant Indexes：SOD activity, CAT activity, MDA contents and GSH activity.It determines The content of glutamic-oxalacetic transaminease AST (GOT), can reflect whether antioxidant damages liver in liver.Anti-oxidant finger Mapping selects Nanjing to build up kit surely.
Variance analysis is carried out to data using SPSS17.0.When difference reaches it is notable when with Tukey inspection carry out multiple ratio Compared with analysis.Different English Superscript letters represent significant difference (P above column diagram<0.05).
If Fig. 1 is shown, there are significant difference (P for the SOD activity of 4 groups of feed and feed 2 groups (high in fat group)<0.05), feed Significant difference (P is not present between 3,4,5 groups>0.05)；There are significant difference (P for the SOD activity of feed 2 and feed 1>0.05).
If Fig. 2 is shown, there are significant difference (P for the CAT activity of 2,3,4 groups of 1 group of feed and feed<0.05), feed 5 is with raising Significant difference (P is not present in CAT activity between expecting 1 group>0.05), but 2,3,4,5 groups of CAT activity of feed there are raising trend.
If Fig. 3 is shown, there are significant difference (P for the MDA contents of 5 groups of 2 groups of feed and feed<0.05), feed 3,4,5 groups it Between MDA contents be not present significant difference (P>0.05), but 3,4,5 groups of MDA contents of feed there are downward trends.
If Fig. 4 is shown, there are significant difference (P for the GSH activity of 2 groups of 3 groups of feed and feed<0.05), feed 3,4,5 groups it Between GSH activity be not present significant difference (P>0.05), but 4,5 groups of GSH activity of feed are above 2 groups of feed.
If Fig. 5 is shown, significant difference (P is not present in the AST activity that 2,3,4,5 groups of feed<0.05), feed 3,4,5 groups it Between AST activity be above 2 groups of feed.
In short, the white lamb's-quarters according to the embodiment 1-3 for showing liver Antioxidant index in above 53,4,5 groups of feed Reed alcohol antioxidant combines the oxidation resistance that can significantly improve Larimichthys crocea, and the effect that 4 groups of feed is more notable, and to big The liver of yellow croaker does not generate apparent side effect, by the way that the rheum officinale fish meal of other embodiments of the invention to be carried out to identical examination Test the identical result that can also obtain.
Although illustrate and describing the present invention with specific embodiment, it will be appreciated that without departing substantially from the present invention's Many other change and modification can be made in the case of spirit and scope.It is, therefore, intended that in the following claims Including belonging to all such changes and modifications in the scope of the invention.

Claims (10)

1. a kind of feed addictive for improving Larimichthys crocea oxidation resistance, which is characterized in that white lamb's-quarters is added in rheum officinale fish meal Reed alcohol, the additive amount of the resveratrol is between the rheum officinale fish meal and the 0.05-0.15wt% of resveratrol gross mass.
2. feed addictive according to claim 1, which is characterized in that the additive amount of the resveratrol is the rheum officinale Between fish meal and the 0.07-0.10wt% of resveratrol gross mass.
3. according to claim 1-2 any one of them feed addictives, which is characterized in that the purity assay of the resveratrol More than 98%.
4. feed addictive according to claim 3, which is characterized in that the purity assay of the resveratrol 99% with On.
5. feed addictive according to claim 4, which is characterized in that the purity assay of the resveratrol is 100%.
6. according to claim 1-5 any one of them feed addictives, which is characterized in that the rheum officinale fish meal mainly wraps It includes：In terms of mass fraction, 20-50 parts of fish meal, 15-20 parts of dregs of beans, 15-20 parts of wheat flour, 1-3 parts of soybean lecithin, fish oil 4-7 Part, 4-7 parts of soya-bean oil, 1-3 parts of mineral substance premix, 0.2-0.4 parts of phagostimulant, 0.05-0.1 parts of ethoxyquin woods, vitamin is pre- 1-3 parts of batch mixing.
7. feed addictive according to claim 6, which is characterized in that the rheum officinale fish meal mainly includes：With quality Number meter, 40 parts of fish meal, 18 parts of dregs of beans, 16 parts of wheat flour, 2 parts of soybean lecithin, 6 parts of fish oil, 6 parts of soya-bean oil, minerals premix 2 parts of material, 0.3 part of phagostimulant, 0.1 part of ethoxyquin woods, 2 parts of vitamin premix.
8. the preparation method of claim 1-7 any one of them feed addictives, which is characterized in that include the following steps：
Resveratrol and rheum officinale fish meal are mixed evenly, you can.
9. the preparation method of feed addictive according to claim 8, which is characterized in that the temperature control of mixing exists 20-30 DEG C, the time control of mixing is in 20-40min.
10. the preparation method of feed addictive according to claim 8, which is characterized in that the rate control of mixing Between 200-300rpm.
CN201810072193.XA 2018-01-25 2018-01-25 A kind of feed addictive for improving Larimichthys crocea oxidation resistance and preparation method thereof Pending CN108157684A (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
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CN111802536A (en) * 2020-07-31 2020-10-23 福建傲农生物科技集团股份有限公司 Feed additive for large yellow croaker female fish and preparation method and application thereof
CN111802536B (en) * 2020-07-31 2022-05-20 福建傲农生物科技集团股份有限公司 Feed additive for large yellow croaker female fish and preparation method and application thereof
CN112056465A (en) * 2020-10-16 2020-12-11 中国水产科学研究院淡水渔业研究中心 Tilapia feed additive, tilapia feed and tilapia feeding method
CN112155122A (en) * 2020-10-20 2021-01-01 中国水产科学研究院淡水渔业研究中心 Chinese herbal medicine additive, tilapia feed and using method thereof
CN116114796A (en) * 2022-10-31 2023-05-16 山东省海洋资源与环境研究院(山东省海洋环境监测中心、山东省水产品质量检验中心) Stress protective agent and preparation method and application thereof
CN116114796B (en) * 2022-10-31 2024-04-02 山东省海洋资源与环境研究院(山东省海洋环境监测中心、山东省水产品质量检验中心) Stress protective agent and preparation method and application thereof
CN115997866A (en) * 2022-12-26 2023-04-25 湖南师范大学 High-fat fish culture feed

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