CN104705532A - Feed additive for improving anti-stress capability of large yellow croakers - Google Patents

Feed additive for improving anti-stress capability of large yellow croakers Download PDF

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Publication number
CN104705532A
CN104705532A CN201510170691.4A CN201510170691A CN104705532A CN 104705532 A CN104705532 A CN 104705532A CN 201510170691 A CN201510170691 A CN 201510170691A CN 104705532 A CN104705532 A CN 104705532A
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China
Prior art keywords
feed
vitamin
stress
yellow croaker
additive
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CN201510170691.4A
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Chinese (zh)
Inventor
张文兵
陈效儒
潘孝毅
张璐
徐玮
麦康森
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中国海洋大学
通威股份有限公司
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Priority to CN201510170691.4A priority Critical patent/CN104705532A/en
Publication of CN104705532A publication Critical patent/CN104705532A/en

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Abstract

The invention aims to provide a feed additive for improving the anti-stress capability of large yellow croakers. The feed additive comprises procyanidine and a vitamin C additive, wherein the mass ratio of procyanidine and the vitamin C additive is preferably (0.5-2):1. The additive amount of procyanidine in the anti-stress additive of large yellow croakers is 100-300mg/kg, and the additive amount of vitamin C phosphate is 500-600mg/kg. The procyanidine and the vitamin C additive combined in the proportion can be used for improving the anti-stress capability and anti-trawling stress capability of the large yellow croakers and does not generate obvious side effects on livers of large yellow croakers.

Description

A kind of feed addictive improving large yellow croaker anti-stress ability
Technical field
The invention belongs to aquatic feeds preparing technical field, be specifically related to a kind of feed addictive improving large yellow croaker anti-stress ability.
Technical background
Large yellow croaker is the maximum marine fish of China's cage culture output, and within 2013, national total output reaches 10.5 ten thousand tons.The cultivation of current large yellow croaker is still based on chilled fish of throwing something and feeding, but the popularity rate of mixed feed reaches 20%.Especially in recent years the system of large yellow croaker nutrient research deeply, succeeded in developing and be seen in market, and the use of mixed feed more and more obtains the approval in market by large yellow croaker special compound feed.In addition chilled fish source is unstable, price fluctuation is large, use inconvenience, easily corruption germinate disease, and resource is caused waste, become increasingly conspicuous to problems such as environment damage, the demand of mixed feed increases gradually, and the spreading speed of mixed feed in large yellow croaker cultivation also can be accelerated.
The problem that large yellow croaker breeding production generally runs into is summer high temperature season because water temperature is higher, and point operation such as fish, draping be frequently subject in the impact of typhoon and breeding process all can produce trawlnet stress, make Pseudosciaena crocea be in stress situation, efficiency of feed utilization reduction will be caused, disease is easily sent out, the death rate increases and grow the generation of unfavorable situations such as slowing down.Therefore, be necessary that exploitation is for high temperature season, the functional feedstuff additive that can promote large yellow croaker anti-stress ability, adjustment feed formula, alleviates and even eliminates stress endangering, for after summer high temperature season, the another fast growing period of large yellow croaker lays the foundation of may occurring this period.Meanwhile, also to improving large yellow croaker Series artificial diets important in inhibiting.
Summary of the invention
The object of this invention is to provide a kind of additive improving large yellow croaker anti-stress ability, thus make up the deficiencies in the prior art.
First the present invention provides a kind of feed addictive, includes OPC and vitamin c class additive.
The mass ratio of its procyanidins and vitamin c class additive is preferably 0.5 ~ 2:1;
Above-mentioned feed addictive is for the preparation of the feed improving large yellow croaker anti-stress ability;
The addition of its procyanidins is preferably 100 ~ 300mg/kg, the addition of vitamin c class additive is 100 ~ 200mg/kg, and the OPC (OPC) under this ratio and vitamin C (VC) can improve the anti-trawlnet stress ability of large yellow croaker.
The one of large yellow croaker feed provided by the invention is specifically composed as follows:
Fish meal 35 ~ 40%, dregs of beans 20 ~ 30%, wheat flour 15 ~ 25%, soybean lecithin 1.5 ~ 2.5%, fish oil 5 ~ 6%, mineral substance premix 1 ~ 3%, glycine 0 ~ 0.1%, betaine 0.2 ~ 0.4%, calcium propionate 0 ~ 0.1%, fumaric acid 0 ~ 0.1%, ethoxyquin woods 0.04 ~ 0.06%, OPC 100 ~ 300mg/kg, vitamin premix 1 ~ 3%; In vitamin premix, the addition of vitamin c class additive is 100 ~ 200mg/kg.
Preferred as embodiment, vitamin c class additive is vitamin C phosphoric ester.
The addition of large yellow croaker resisting stress additive procyanidins of the present invention is 100 ~ 300mg/kg, the addition of vitamin C phosphoric ester is 500 ~ 600mg/kg, OPC (OPC) under this ratio and vitamin C (VC) combination can improve the anti-oxidation stress ability of large yellow croaker and anti-trawlnet stress ability, and do not produce obvious side effect to the liver of large yellow croaker.
Accompanying drawing explanation
Fig. 1: resisting stress additive is on the impact of liver SOD activity;
Fig. 2: resisting stress additive is on the impact of liver CAT activity;
Fig. 3: resisting stress additive is on the impact of blood-sugar content; Wherein different lowercase subscript represent stress before significant difference (P<0.05) between different disposal group, different capitalization subscript represent stress post processing group difference significantly (P<0.05); T checks: P be same processed group trawlnet stress before and after fiducial value.
Fig. 4: resisting stress additive to the effect diagram of Determination of cortisol,
Wherein: Tukey Multiple range test: different lowercase subscript represent stress before significant difference (P<0.05) between different disposal group, different capitalization subscript represent stress post processing group difference significantly (P<0.05); T checks: P be same processed group trawlnet stress before and after fiducial value.
Fig. 5: resisting stress additive is on the impact of serum GOT activity;
Fig. 6: resisting stress additive is on the impact of serum GPT activity.
Detailed description of the invention
Applicant is found by long-term research, by OPC and ascorbic synergy, can effectively improve large yellow croaker anti-stress ability, thus facilitate the present invention.
Below in conjunction with embodiment, the present invention is described in detail.
Embodiment 1:
There are some researches show, with specific growth rate (SGR) for evaluation index large yellow croaker is 60 ~ 75mg/kg feed to vitamin C requirements amount.
1# feed is the amount (283mg/kg) of adding the VC phosphate just meeting large yellow croaker growth demand, and OPC addition is the processed group of 0; The addition of 2# feed VC phosphate is the twice (566mg/kg) of growth demand, and OPC addition is the processed group of 0; 3# feed OPC and Vc phosphate be combined as (100OPC+283VC) mg/kg, wherein the addition of VC phosphate is growth demand; 4# feed OPC and Vc phosphate be combined as (100OPC+566VC) mg/kg, wherein the addition of VC phosphate is the twice of growth demand.In the VC phosphate of wherein 283mg/k, the actual addition of VC is the actual addition of VC in the VC phosphate of 69mg/kg, 566mg/k is 138mg/kg.With above-mentioned experiment feed (referring to table 1), carry out culture experiment.
Table 1: experiment feed formula
Wherein mineral substance premix (mg/kg): epsom salt (MgSO 4.7H 2o), 180; Cupric sulfate pentahydrate (CuSO 4.5H 2o), 2.5; Ferrous sulfate monohydrate (FeSO 4.H 2o), 24; Monohydrate zinc sulphate (ZnSO 4.H 2o), 17.25; Manganese sulfate monohydrate (MnSO 4.H 2o), 14.31; Cobalt chloride (CoCl 2), 0.5; Sodium selenite (Na 2seO 3), 0.2; Calcium iodate (Ca (IO 3) 2.6H 2o), 0.6; Calcium dihydrogen phosphate 10000 microcrystalline cellulose, 8485.But also can select mineral mixture conventional in large yellow croaker feed;
Vitamin premix (mg/kg): VAC, 16000IU; D3 particulate, 2500IU; α ?tocopherol, 120; Menadione sodium bisulfite (MSB), 5.1; Thiamine hydrochloride, 24.5; Riboflavin particulate, 36; Pyridoxol, 19.8; Cyanocobalamin, 0.1; D ?calcium pantothenate, 58.8; Nicotinic acid, 198; Folic acid, 19.6; Biotin, 1.2; Inositol, 784; Choline Chloride, 2000.Also the vitamin mixtures in other commercially available aquatic products field can be used.
Select the same a collection of large yellow croaker of artificial incubation then as experiment fish, average weight is 130.35 ± 8.37g.Pick out consistent, the athletic large yellow croaker of specification to put in a suitable place to breed in 12 specifications to be in the seawater floating cage of 2m × 2m × 2.5m, in each net cage, to put 50 tails in a suitable place to breed.Often kind of feedstuff feeding 3 net cages, culture experiment carries out 30 days altogether.Stress tests is carried out after culture experiment terminates.
Stress tests be trawlnet stress:
Trawlnet stress the trawlnet that is operating as just leave water by fish, air exposure 10s, then net put down allow fish mention again after 10s in water, repeat 3 times, blood sampling after 30min.
Glucose in serum and cortisol are the important indicators evaluating fish anti-stress ability, when fish are stimulated by stressor, Determination of cortisol can significantly raise, and then promotes that blood-sugar content increases, blood sugar increase to muscle and nerve provides more multi-energy, make fish adapt to stress environment.On this basis, fish body creates adaptability to stressor, and its biological function recovers normal; Or with stress the intensification of degree, the such as growth rate that fish individual or population level occur, the change such as fertility and resistance against diseases reduction.
Glutamic-oxalacetic transaminease is mainly present in liver cell mitochondria, when liver generation severe necrosis or when destroying, glutamic-oxalacetic transaminease concentration in serum just can be caused higher, if the content of glutamic-oxalacetic transaminease increases severely in serum, possibility liver generation severe necrosis or destruction can be inferred, therefore can be used to judge whether liver suffers damage by the content measuring glutamic-oxalacetic transaminease in serum.Glutamic-pyruvic transaminase (GPT) is mainly present in liver, heart and skeletal muscle.Liver cell or some tissue damage or necrosis, all can make the glutamic-pyruvic transaminase in blood raise, therefore GPT content also deducibility liver whether receive damage.
Measure that four processed group liver Antioxidant Indexes: SOD are active, CAT is active, stress before and after the content of cortisol, blood sugar in large yellow croaker serum, thus reflect its anti-trawlnet stress ability.Determine the content of glutamic-oxalacetic transaminease in serum (GOT), glutamic-pyruvic transaminase (GPT), can reflect whether resisting stress additive damages liver.SOD, CAT, GOT, GPT, cortisol measure selects Nanjing to build up kit.The mensuration of glucose selects hexokinase method, and use instrument is Hitachi Hitachi7600 Biochemical Analyzer.
SPSS17.0 is adopted to carry out variance analysis to data.Multiple comparison analyse is carried out with Tukey inspection when difference reaches remarkable.Above column diagram, different English Superscript letters indicates significant difference (P<0.05), and t checks: P be same processed group stress before and after fiducial value.
As Fig. 1 display, there is significant difference (P<0.05) with the SOD of feed 1 (control group) in 3,4 groups, feed, there is not significant difference (P>0.05) between this 2 feed group; Significant difference (P>0.05) is there is not in feed 2 with the SOD activity of feed 1.
Fig. 2 shows, there is significant difference (P<0.05) in the CAT activity of 4 groups, feed and feed 1 group (control group), between feed 3 and feed 1, feed 4 there is not significant difference (P>0.05) in CAT activity, and feed 2 does not exist significant difference (P>0.05) with the CAT activity of feed 1.
The oxidation resistance of large yellow croaker can be significantly improved, the more remarkable effect that feed is 4 groups according to the OPC and ascorbic resisting stress additive combination that the activity analysis of SOD and CAT are shown to 3,4 groups, feed.
Fig. 3 display stress before, feed 2,3,4 groups of blood-sugar contents are significantly higher than 1 group, feed (P<0.05); Stress after, feed 3,4 groups of large yellow croaker blood-sugar contents are significantly higher than 1,2 groups, feed (P<0.05).
Same processed group large yellow croaker trawlnet stress before and after blood-sugar content compare, feed 1,2,3 groups of large yellow croakers trawlnet stress before and after blood-sugar content there were significant differences (P1=0.007, P2=0.001, P3=0.001); Feed 4 groups of large yellow croakers trawlnet stress before and after blood-sugar content there is no significant difference (, P4=0.064).
Fig. 4 display stress before, feed 2,3,4 groups of Determination of cortisols are significantly higher than 1 group, feed (P<0.05); Stress after, feed 3,4 groups of large yellow croaker Determination of cortisols are also significantly higher than 1,2 groups, feed (P<0.05).
Same processed group large yellow croaker trawlnet stress before and after Determination of cortisol compare, each feed group large yellow croaker trawlnet stress before and after Determination of cortisol all there were significant differences (P<0.05).Feed 1 group of trawlnet stress add 408.2% by front and back Determination of cortisol, and feed 2,3,4 groups of Determination of cortisols add 65.47%, 41.4%, 52.14% respectively.
Experiment show 4 groups, feed trawlnet stress before and after, glucose content in serum without significant difference (P>0.05), and 3,4 groups, feed stress before and after the percentage of cortisol levels have also been obtained obvious reduction relative to 1 group, feed.Show feed 4 groups of large yellow croaker trawlnets stress before and after blood sugar and cortisol levels little, this illustrates that feed 4 groups of OPCs and ascorbic resisting stress additive combination can improve the anti-stress ability of large yellow croaker.
Fig. 5 shows 1,2,3,4 groups, feed and does not cause remarkable impact (P>0.05) to large yellow croaker serum glutamic oxalacetic transaminase activity.
Fig. 6 shows, 1,2,3,4 groups, feed does not cause remarkable impact (P>0.05) to large yellow croaker serum glutamic pyruvic transminase activity, illustrates that OPC prepared by use the present invention and vitamin C combination resisting stress additive do not produce obvious side effect to large yellow croaker liver.
In sum, the resisting stress additive that feed 4 groups of OPCs and vitamin C combine, namely the resisting stress additive of (100OPC+566VC) mg/kg can significantly improve the anti-oxidant of large yellow croaker and anti-trawlnet stress ability, and does not produce obvious side effect to the liver of large yellow croaker.
The additive that OPC and vitamin c class additive different ratio are made is detected, shows that all there is the ability improving large yellow croaker resisting stress.

Claims (8)

1. a feed addictive, is characterized in that, described feed addictive includes OPC and vitamin c class additive.
2. feed addictive as claimed in claim 1, it is characterized in that, described feed addictive procyanidins and the mass ratio of vitamin c class additive are 0.5 ~ 2:1.
3. feed addictive as claimed in claim 1 or 2, it is characterized in that, described vitamin c class additive is vitamin C phosphoric ester.
4. feed addictive according to claim 1 is for the preparation of the feed of large yellow croaker.
5. a large yellow croaker feed, is characterized in that, is added with feed addictive according to claim 1 in described large yellow croaker feed.
6. large yellow croaker feed as claimed in claim 5, it is characterized in that, described feed addictive, the addition of its procyanidins is preferably 100 ~ 300mg/kg, and the addition of vitamin c class additive is 100 ~ 200mg/kg.
7. large yellow croaker feed as claimed in claim 5, is characterized in that, described large yellow croaker feed composed as follows:
Fish meal 35 ~ 40%, dregs of beans 20 ~ 30%, wheat flour 15 ~ 25%, soybean lecithin 1.5 ~ 2.5%, fish oil 5 ~ 6%, mineral substance premix 1 ~ 3%, glycine 0 ~ 0.1% betaine 0.2 ~ 0.4%, calcium propionate 0 ~ 0.1%, fumaric acid 0 ~ 0.1%, ethoxyquin woods 0.04 ~ 0.06%, OPC 100 ~ 300mg/kg, vitamin premix 1 ~ 3%; In vitamin premix, the addition of vitamin c class additive is 100 ~ 200mg/kg.
8. the large yellow croaker feed as described in any one of claim 5-7, is characterized in that, described vitamin c class additive is vitamin C phosphoric ester.
CN201510170691.4A 2015-04-12 2015-04-12 Feed additive for improving anti-stress capability of large yellow croakers CN104705532A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104982760A (en) * 2015-08-07 2015-10-21 盐城工学院 Anti-stress low fish meal compound feed for pikes and production method thereof
CN105941198A (en) * 2016-04-22 2016-09-21 苏州依科曼生物农业科技有限公司 Micro-ecological mixed preparation and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1736241A (en) * 2005-09-01 2006-02-22 中国海洋大学 Artificial micro-particle feedstuff for pseudosciaenacrocea larvae
CN102038109A (en) * 2010-12-20 2011-05-04 宁波大学 Antistress reinforcing agent for large yellow croaker
JP4863615B2 (en) * 2004-12-16 2012-01-25 日清丸紅飼料株式会社 Functional fish feed

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4863615B2 (en) * 2004-12-16 2012-01-25 日清丸紅飼料株式会社 Functional fish feed
CN1736241A (en) * 2005-09-01 2006-02-22 中国海洋大学 Artificial micro-particle feedstuff for pseudosciaenacrocea larvae
CN102038109A (en) * 2010-12-20 2011-05-04 宁波大学 Antistress reinforcing agent for large yellow croaker

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
卢俊蛟等: "饲料中添加葡多酚对吉富罗非鱼生长性能、肠道消化酶活性、血脂水平和肝胰脏抗氧化能力的影响", 《动物营养学报》, vol. 26, no. 4, 30 April 2014 (2014-04-30) *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104982760A (en) * 2015-08-07 2015-10-21 盐城工学院 Anti-stress low fish meal compound feed for pikes and production method thereof
CN105941198A (en) * 2016-04-22 2016-09-21 苏州依科曼生物农业科技有限公司 Micro-ecological mixed preparation and application thereof

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