CN108271980A - A kind of ginseng beverage and its preparation method and application - Google Patents
A kind of ginseng beverage and its preparation method and application Download PDFInfo
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- CN108271980A CN108271980A CN201810133872.3A CN201810133872A CN108271980A CN 108271980 A CN108271980 A CN 108271980A CN 201810133872 A CN201810133872 A CN 201810133872A CN 108271980 A CN108271980 A CN 108271980A
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- glycoprotein
- krill
- parts
- ginseng
- water
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- 241000208340 Araliaceae Species 0.000 title claims abstract description 110
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 title claims abstract description 110
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- 235000013361 beverage Nutrition 0.000 title claims abstract description 97
- 238000002360 preparation method Methods 0.000 title claims abstract description 29
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- 229940035035 polydextrose Drugs 0.000 claims abstract description 27
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 52
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- 239000007864 aqueous solution Substances 0.000 claims description 28
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- 239000000843 powder Substances 0.000 claims description 24
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- QEWYKACRFQMRMB-UHFFFAOYSA-N fluoroacetic acid Chemical compound OC(=O)CF QEWYKACRFQMRMB-UHFFFAOYSA-N 0.000 description 6
- 235000019419 proteases Nutrition 0.000 description 6
- 244000189799 Asimina triloba Species 0.000 description 5
- 235000006264 Asimina triloba Nutrition 0.000 description 5
- 235000009467 Carica papaya Nutrition 0.000 description 5
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- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000628997 Flos Species 0.000 description 1
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- 241000123870 Geum aleppicum Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
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- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/04—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from fish or other sea animals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
- A23L2/66—Proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Zoology (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Marine Sciences & Fisheries (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Non-Alcoholic Beverages (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention provides a kind of ginseng beverage, the ginseng beverage includes 0.5 10 parts of ginseng by weight；38 parts of krill glycoprotein；0.5 2 parts of polydextrose；220 290 parts of water；The present invention also provides a kind of preparation method of ginseng beverage and purposes；Ginseng beverage provided by the invention, traditional Chinese medicine and krill glycoprotein are compounded, so that krill glycoprotein can be also fully absorbed, not only there is important health value, and the antitumor activity of krill glycoprotein can be given full play to and improve the effect of blood vessel, the dispersion stabilization of beverage is improved by the addition of polydextrose in beverage in addition, the quality of beverage is improved, more there is tasty and refreshing feeling.
Description
Technical field
The invention belongs to the field of Chinese medicines, it is related to a kind of ginseng beverage and its preparation method and application.
Background technology
Ginseng is perennial herb, and ginseng main root is 30-60 centimetres high, plump, meat, yellow-white, cylindrical or spinning
Capitate, below slightly branch；Root-like stock (reed head) is short, uprightly, with beauty and medicinal efficacy, can moisturize the skin, clinical
On have certain positive effect for treatment disease of cardiovascular system, stomach and a variety of diseases such as liver diseases and diabetes, and
The acute of anoxic, chronic plateau sickness etc. can be prevented, but the factors such as ginseng inconvenient eating limit ginseng trend greatly at present
It is many.
CN106722938A discloses a kind of ginseng beverage, is made of the raw material of following parts by weight：90~120 parts of ginseng,
5~10 parts of CORTEX PHELLODENDRI AMURENE, 5~10 parts of aleppo avens, 1~3 part of the root of bidentate achyranthes, 1~3 part of Chinese yam, 1~3 part of Fructus Caryophylli, 1~3 part of Flos Magnoliae Officinalis,
1~3 part of kamuning, such beverage enhances human immunological competence, but this beverage ingredient is excessively complicated, and adjunct ingredient may
The health value of ginseng can be impacted, certain side effect may be generated to human body.
At present all there is certain defect in the beverage of ginseng, thus how to develop it is a kind of it is novel, drunk convenient for masses
Beverage and the health-care hospital effect for further promoting beverage, have important market value.
Invention content
The purpose of the present invention is to provide a kind of ginseng beverages, allow water to return ontology, highlight healthy water, be really achieved
Healthy moisturizing, the purpose to regain one's strength.
For this purpose, the present invention uses following technical scheme：
In a first aspect, the present invention provides a kind of ginseng beverage, the ginseng beverage includes ginseng 2-8 by weight
Part；1-3 parts of krill glycoprotein；0.5-2 parts of polydextrose；240-280 parts of water.
Ginseng beverage provided by the invention compounds traditional Chinese medicine and krill glycoprotein so that krill glycoprotein also may be used
It is fully absorbed, not only there is important health value, and the antitumor activity of krill glycoprotein can be given full play to and changed
The effect of kind blood vessel, improves the dispersion stabilization of beverage, improves beverage in beverage by the addition of polydextrose in addition
Quality more has tasty and refreshing feeling.
In the present invention, the weight content of the ginseng is 2-8 parts, for example, can be 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7
Part or 8 parts.
In the present invention, the weight content of the krill glycoprotein is 1-3 parts, for example, can be 1 part, 1.5 parts, 2 parts,
2.5 parts or 3 parts.
In the present invention, krill glycoprotein is cooperateed with ginseng plays effect, not only makes beverage with health role, but also have
There is certain antitumor activity, and lack any one component, the nutritive value of beverage can be made to be greatly reduced with health-care efficacy.
In the present invention, the weight content of the polydextrose is 0.5-2 parts, such as can be 0.5 part, 1 part, 1.5 parts
Or 2 parts.
In the present invention, the addition of polydextrose so that without adding any other stabilizer in beverage, so that it may to change
The dispersibility and stability of kind beverage more have tasty and refreshing feeling so that the quality of beverage is further promoted.
In the present invention, the weight content of the water is 240-280 parts, for example, can be 240 parts, 245 parts, 250 parts,
255 parts, 260 parts, 265 parts, 270 parts or 280 parts.
Preferably, the ginseng beverage includes 0.8-8 parts of ginseng by weight；4-6 parts of krill glycoprotein；Polydextrose
1-2 parts；240-280 parts of water.
In the present invention, it is preferred to which the ginseng beverage of this range, can be such that health care, the effect of moisturizing and mouthfeel reaches most
Good, the parts by weight of most preferably ginseng, krill glycoprotein and water are respectively 4 parts, 3 parts and 265 parts, and the effect that nutritional ingredient incorporates is most
Good, health-care effect is best.
Preferably, the ginseng beverage further includes 1-3 parts of shark glycoprotein, for example, can be 1 part, 1.5 parts, 2 parts, 2.5
Part or 3 parts.
In the present invention, shark glycoprotein can further strengthen the health-care effect of beverage, by testing surface, shark sugar
Albumen has obvious inhibiting effect, extracorporeal anti-tumor to show new vessels, has apparent inhibiting effect to tumour cell, and
Shark glycoprotein and krill glycoprotein, which can mutually promote, plays antitumous effect, and when individually adding one of which, effect
Decline.
In the present invention, the krill glycoprotein is prepared by krill by enzymolysis and extraction.
Preferably, the method for enzymolysis and extraction krill glycoprotein includes the following steps：
(1) krill meat is homogenized in a solvent, adjusts pH value and homogenate concentrations, supernatant is collected by centrifugation for the first time, continued
Concentration is adjusted with salt, precipitation is collected by centrifugation for the second time；
(2) precipitation that step (1) obtains is handled using solvent, filtering freeze-drying obtains degreasing protein powder；
(3) the degreasing protein powder obtained step (2) is soluble in water, adjusts pH value, and enzyme is added and is digested, after enzymolysis
Product destroy the enzyme treatment obtain enzymolysis liquid, enzymolysis liquid is centrifuged to obtain supernatant, krill glycoprotein crude product is obtained by processing；
(4) the krill glycoprotein crude product that step (3) obtains is soluble in water, and crude krill glycoprotein group is obtained by column chromatography
Point, then pass through sephadex column after purification, reuses high performance liquid chromatography and be further purified, obtain the krill sugar egg
In vain.
Preferably, solvent described in step (1) is ethyl alcohol.
Preferably, a concentration of 75%-85% of the ethyl alcohol, for example, can be 75%, 78%, 80%, 82%, 83%,
84% or 85%.
Preferably, the reagent that pH value is adjusted described in step (1) is sodium hydroxide.
Preferably, adjusted described in step (1) pH value be adjusted to 7.1-8.8, such as can be 7.1,7.4,7.5,
7.8,8,8.1,8.3 or 8.8.
Preferably, it is using water tune concentration of alcohol to 35%-42% that homogenate concentrations are adjusted described in step (1), such as can
To be 35%, 36%, 37%, 38%, 40%, 41% or 42%.
Preferably, further include keeping the temperature 15min- in 25 DEG C of -35 DEG C of water-baths before being centrifuged for the first time described in step (1)
25min。
Preferably, salt described in step (1) is sodium chloride.
Preferably, the sodium chloride adjusts the concentration of supernatant to 10%-15%, for example, can be 10%, 11%,
12%, 13%, 14% or 15%.
Preferably, further include 15 DEG C of -20 DEG C of water-baths heat preservation 6h-9h after salt described in step (1) adjusts concentration.
Preferably, the rotating speed of second of centrifugation described in step (1) is 3000r/min-5000r/min, such as can be
3000r/min, 3200r/min, 3500r/min, 4000r/min, 4500r/min or 5000r/min.
Preferably, the time of second centrifugation described in step (1) is 4min-10min, for example, can be 4min, 5min,
6min, 7min, 8min, 9min or 10min.
Preferably, solvent described in step (2) is ether.
Preferably, the mass ratio of defatted protein powder and water described in step (3) is 1:(42-66), such as can be 1:42、
1:44、1:45、1:50、1:55、1:60、1:63 or 1:66.
Preferably, the reagent that pH value is adjusted described in step (3) is hydrochloric acid.
Preferably, described in step (3) adjust pH value be adjusted to 3-5.5, such as can be 3,4,4.5,4.8,5 or
5.5。
Preferably, enzyme described in step (3) is papain.
In the present invention, use papain that can make the krill glycoprotein being prepared that there is high nutritive value
And health-care effect, and digested using other enzymes, then optimal effect is not achieved.
Preferably, the enzyme activity of the papain is more than 2.5 × 106U/g。
Preferably, the quality of enzyme described in step (3) is the 0.5%-0.7% of degreasing protein sub-prime amount.
Preferably, the time digested described in step (3) be 2h-4h, such as can be 2h, 2.2h, 2.5h, 2.8h, 3h,
3.5h or 4h.
Preferably, the temperature digested described in step (3) is 36 DEG C -50 DEG C, for example, can be 36 DEG C, 38 DEG C, 40 DEG C, 45
DEG C, 48 DEG C or 50 DEG C.
Preferably, processing described in step (3) includes desalination, concentration and freeze-drying.
Preferably, column chromatography described in step (4) chromatographs for anion exchange resin.
Preferably, the anion exchange resin is DEAE-cellulose-52.
Preferably, sephadex described in step (4) is Sephadex G-100.
Preferably, the chromatographic column of high performance liquid chromatography described in step (4) is Inertsil ODS-3C18Column.
Preferably, the type of elution of column chromatography described in step (4) is：First washed with 0.13mol/L-0.18mol/L NaCl
It is de-, it then uses 0.3mol/L-0.4mol/L NaCl elutions, collects 0.3mol/L-0.4mol/L NaCl elution fractions, i.e., it is crude
Krill glycoprotein fraction.
In the present invention, by krill by using degreasing, enzymolysis, ion exchange resin column chromatography, gel filtration chromatography and anti-
The glycoprotein that phase high-efficient liquid phase chromatogram purification is prepared, concentration purity is high, for improving human vas, promotes health-care effect tool
There is the effect of highly significant.
In the present invention, the preparation method of shark glycoprotein is identical as the preparation method of krill glycoprotein, again by enzyme
Solution is prepared.
Preferably, the ginseng beverage includes 2-8 parts of ginseng；1-3 parts of krill glycoprotein；1-3 parts of shark glycoprotein；Poly- Portugal
0.5-2 parts of grape sugar；240-280 parts of water.
It is further preferred that the ginseng beverage includes 3-8 parts of ginseng；2-3 parts of krill glycoprotein；Shark glycoprotein 2-3
Part；1-2 parts of polydextrose；240-280 parts of water.
Second aspect, the present invention provides a kind of preparation method of ginseng beverage as described in relation to the first aspect, the methods
Include the following steps：By ginseng, krill glycoprotein, polydextrose and water and optionally shark glycoprotein mixing, digestion, sterilization
Obtain the ginseng beverage.
Preferably, it the described method comprises the following steps：
(a) krill glycoprotein aqueous solution and shark is respectively prepared after enzymolysis and extraction in krill glycoprotein and shark glycoprotein
Fish glycoprotein aqueous solution；
(b) ginseng crushing, distillation are obtained into powder, krill glycoprotein aqueous solution and shark glycoprotein aqueous solution is added, and
Polydextrose mixing is added afterwards, digestion obtains the ginseng beverage after sterilization.
Concentration of aqueous solution described in step (a) be 0.5mg/L-5mg/L, such as can be 0.5mg/L, 1mg/L, 2mg/L,
3mg/L, 4mg/L or 5mg/L.
In the present invention, the concentration of aqueous solution of krill glycoprotein and shark glycoprotein is specific, if excessive concentration, meeting
The effect of inhibiting ginseng, and if concentration is too low, its antitumor effect cannot be played.
Preferably, the temperature of digestion described in step (b) is 80 DEG C -100 DEG C, for example, can be 80 DEG C, 82 DEG C, 85 DEG C,
86 DEG C, 88 DEG C, 90 DEG C, 95 DEG C, 96 DEG C, 98 DEG C or 100 DEG C, preferably 100 DEG C.
Preferably, the time of digestion described in step (b) be 16min-18min, such as can be 16min, 16.2min,
16.5min, 17min, 17.3min, 17.5min or 18min.
In the present invention, by controlling temperature and incorporation time, ensure that the main ingredient of ginseng is not destroyed, Ke Yichong
Divide, equably incorporate in mixed liquor；Temperature is excessively high, can destroy the main component of ginseng, and temperature is too low, then cannot make mainly at
Divide and fully incorporates in mixed liquor；Time is too short, is equally not achieved the effect fully dissolved, overlong time, will also result in mainly at
The destruction divided；It is preferred that 100 DEG C, optimum efficiency can be reached.
Preferably, the method sterilized described in step (b) is that ultrasonic sterilization or electromagnetic field are sterilized.
Preferably, the time sterilized described in step (b) be 26min-68min, such as can be 26min, 30min,
35min, 40min, 45min, 50min, 55min, 60min, 62min or 68min.
As optimal technical scheme, ginseng beverage preparation method provided by the invention includes the following steps：
(a) krill glycoprotein aqueous solution and shark is respectively prepared after enzymolysis and extraction in krill glycoprotein and shark glycoprotein
Fish glycoprotein aqueous solution；
(b) ginseng crushing, distillation are obtained into powder, krill glycoprotein aqueous solution and shark glycoprotein aqueous solution is added, and
Polydextrose mixing is added afterwards, digestion obtains the ginseng beverage after sterilization.
The third aspect, the present invention provides a kind of ginseng beverage as described in relation to the first aspect prepare anti-fatigue health-product containing or
Purposes in nourishment for vitality health products.
Ginseng beverage provided by the invention may also be fabricated which with the productions such as antifatigue health products or nourishment for vitality health products
Product are mutually promoted especially because the collocation of both krill glycoprotein and shark glycoprotein uses, and reasonably combined ginseng uses,
So that beverage has good antitumor efficacy, it can be used as a kind of health products of adjuvant therapy of tumors and drink.
Compared with the existing technology, the invention has the advantages that：
(1) this ginseng beverage provided by the invention, traditional Chinese medicine and krill glycoprotein is compounded so that krill glycoprotein
Also it can be fully absorbed, not only there is important health value, and the antitumor activity of krill glycoprotein can be given full play to
Effect with blood vessel is improved, improves the dispersion stabilization of beverage by the addition of polydextrose in beverage in addition, improves drink
The quality of material more has tasty and refreshing feeling.
(2) ginseng beverage provided by the invention improves people by the reasonably combined of shark glycoprotein and krill glycoprotein
The health value of ginseng beverage mutually promotes and plays antitumor effect, compared to existing ginseng beverage, has expanded and has applied model
It encloses, on the basis of keeping mouthfeel, has both antitumous effect, the rehabilitation of body can be promoted, had broad application prospects.
Specific implementation mode
The technical solution further illustrated the present invention below by specific implementation mode.Those skilled in the art should be bright
, the embodiment, which is only to aid in, understands the present invention, should not be regarded as a specific limitation of the invention.
Embodiment 1
The present embodiment prepares ginseng beverage by following steps, including 3 parts of ginseng, 3 parts of krill glycoprotein, poly- grape
1 part of sugar, 268 parts of water；
Ginseng crushing, distillation are obtained into powder, krill glycoprotein aqueous solution is added, polydextrose is then added and mixes, 100
DEG C digestion 18min, ginseng beverage is obtained after 30min is sterilized.
The wherein preparation method of krill glycoprotein, includes the following steps：
(1) krill meat is homogenized in ethanol, pH to 8 is adjusted with the sodium hydroxide solution of 1mol/L, then with water tune second
Determining alcohol keeps the temperature 15min-25min in 25 DEG C of -35 DEG C of water-baths, supernatant is collected by centrifugation for the first time to 40%, continues to use chlorination
Sodium adjusts concentration and centrifuges 7min for the second time to 13%, 4000r/min, collects precipitation；
(2) obtained precipitation is handled into 2h using ether, filtering freeze-drying obtains degreasing protein powder；
(3) it is 1 according to solid-liquid ratio by obtained degreasing protein powder:45 is soluble in water, adjusts pH to 5, and pawpaw egg is added
White enzyme is digested, and 3h is digested in 45 DEG C, and the product destroy the enzyme treatment after enzymolysis obtains enzymolysis liquid, enzymolysis liquid is centrifuged to obtain supernatant
Liquid obtains krill glycoprotein crude product by processing；
(4) the krill glycoprotein crude product that step (3) obtains is soluble in water, is handed over by DEAE-cellulose-52 anion
Resin chromatography is changed, 0.15mol/L NaCl elutions are first used, 0.35mol/L NaCl elutions is then used, collects 0.35mol/L NaCl
Elution fraction obtains crude krill glycoprotein fraction, then passes through Sephadex G-100 sephadex columns after purification, then make
It is further purified with high performance liquid chromatography, chromatographic column is Inertsil ODS-3C18Column, mobile phase are acetonitrile and water (0.1% 3
Fluoroacetic acid, flow velocity 1.5mL/min), obtain krill glycoprotein.
Embodiment 2
The present embodiment prepares ginseng beverage by following steps, including 6 parts of ginseng, 1 part of krill glycoprotein, poly- grape
0.5 part of sugar, 270 parts of water；
Ginseng crushing, distillation are obtained into powder, krill glycoprotein aqueous solution is added, polydextrose is then added and mixes, 100
DEG C digestion 16min, obtains the ginseng beverage after 26min is sterilized.
The wherein preparation method of krill glycoprotein, includes the following steps：
(1) krill meat is homogenized in ethanol, adjusts pH value to 8.8 with the sodium hydroxide solution of 1mol/L, then uses water
It adjusts concentration of alcohol to 42%, 15min-25min is kept the temperature in 25 DEG C of -35 DEG C of water-baths, supernatant is collected by centrifugation for the first time, continues to use
Sodium chloride adjusts concentration and centrifuges 10min for the second time to 15%, 3000r/min, collects precipitation；
(2) obtained precipitation is handled into 2h using ether, filtering freeze-drying obtains degreasing protein powder；
(3) it is 1 according to solid-liquid ratio by obtained degreasing protein powder:42 is soluble in water, adjusts pH value to 3, pawpaw is added
Protease is digested, and 2h is digested in 45 DEG C, and the product destroy the enzyme treatment after enzymolysis obtains enzymolysis liquid, enzymolysis liquid is centrifuged to obtain
Clear liquid obtains krill glycoprotein crude product by processing；
(4) the krill glycoprotein crude product that step (3) obtains is soluble in water, is handed over by DEAE-cellulose-52 anion
Resin chromatography is changed, 0.15mol/L NaCl elutions are first used, 0.35mol/L NaCl elutions is then used, collects 0.35mol/L NaCl
Elution fraction obtains crude krill glycoprotein fraction, then passes through Sephadex G-100 sephadex columns after purification, then make
It is further purified with high performance liquid chromatography, chromatographic column is Inertsil ODS-3C18Column, mobile phase are acetonitrile and water (0.1% 3
Fluoroacetic acid, flow velocity 1.5mL/min), obtain krill glycoprotein.
Embodiment 3
The present embodiment prepares ginseng beverage by following steps, including 4 parts of ginseng, 3 parts of krill glycoprotein, shark sugar
2 parts of albumen, 2 parts of polydextrose, 265 parts of water；
(1) krill glycoprotein aqueous solution and shark is respectively prepared after enzymolysis and extraction in krill glycoprotein and shark glycoprotein
Fish glycoprotein aqueous solution；
(2) ginseng crushing, distillation are obtained into powder, krill glycoprotein aqueous solution and shark glycoprotein aqueous solution is added, and
Polydextrose mixing is added afterwards, 80 DEG C of digestion 16min obtain ginseng beverage after 68min is sterilized.
The wherein preparation method (wherein the preparation method of shark glycoprotein is identical as krill glycoprotein) of krill glycoprotein, packet
Include following steps：
(1) krill meat is homogenized in ethanol, adjusts pH value to 7.1 with the sodium hydroxide solution of 1mol/L, then uses water
It adjusts concentration of alcohol to 35%, 15min-25min is kept the temperature in 25 DEG C of -35 DEG C of water-baths, supernatant is collected by centrifugation for the first time, continues to use
Sodium chloride adjusts concentration and centrifuges 4min for the second time to 10%, 3000r/min, collects precipitation；
(2) obtained precipitation is handled into 2h using ether, filtering freeze-drying obtains degreasing protein powder；
(3) it is 1 according to solid-liquid ratio by obtained degreasing protein powder:42 is soluble in water, adjusts pH value to 3, pawpaw is added
Protease is digested, and 2h is digested in 36 DEG C, and the product destroy the enzyme treatment after enzymolysis obtains enzymolysis liquid, enzymolysis liquid is centrifuged to obtain
Clear liquid obtains krill glycoprotein crude product by processing；
(4) the krill glycoprotein crude product that step (3) obtains is soluble in water, is handed over by DEAE-cellulose-52 anion
Resin chromatography is changed, 0.15mol/L NaCl elutions are first used, 0.35mol/L NaCl elutions is then used, collects 0.35mol/L NaCl
Elution fraction obtains crude krill glycoprotein fraction, then passes through Sephadex G-100 sephadex columns after purification, then make
It is further purified with high performance liquid chromatography, chromatographic column is Inertsil ODS-3C18Column, mobile phase are acetonitrile and water (0.1% 3
Fluoroacetic acid, flow velocity 1.5mL/min), obtain krill glycoprotein.
Embodiment 4
The present embodiment prepares ginseng beverage by following steps, including 8 parts of ginseng, 3 parts of krill glycoprotein, shark sugar
3 parts of albumen, 1.5 parts of polydextrose, 280 parts of water；
(1) krill glycoprotein aqueous solution and shark is respectively prepared after enzymolysis and extraction in krill glycoprotein and shark glycoprotein
Fish glycoprotein aqueous solution；
(2) ginseng crushing, distillation are obtained into powder, krill glycoprotein aqueous solution and shark glycoprotein aqueous solution is added, and
Polydextrose mixing is added afterwards, 80 DEG C of digestion 16min obtain ginseng beverage after 68min is sterilized.
The wherein preparation method (wherein the preparation method of shark glycoprotein is identical as krill glycoprotein) of krill glycoprotein, packet
Include following steps：
(1) krill meat is homogenized in ethanol, adjusts pH value to 8.8 with the sodium hydroxide solution of 1mol/L, then uses water
It adjusts concentration of alcohol to 42%, 15min-25min is kept the temperature in 25 DEG C of -35 DEG C of water-baths, supernatant is collected by centrifugation for the first time, continues to use
Sodium chloride adjusts concentration and centrifuges 10min for the second time to 15%, 5000r/min, collects precipitation；
(2) obtained precipitation is handled into 2h using ether, filtering freeze-drying obtains degreasing protein powder；
(3) it is 1 according to solid-liquid ratio by obtained degreasing protein powder:40 is soluble in water, adjusts pH value to 4, pawpaw is added
Protease is digested, and 4h is digested in 50 DEG C, and the product destroy the enzyme treatment after enzymolysis obtains enzymolysis liquid, enzymolysis liquid is centrifuged to obtain
Clear liquid obtains krill glycoprotein crude product by processing；
(4) the krill glycoprotein crude product that step (3) obtains is soluble in water, is handed over by DEAE-cellulose-52 anion
Resin chromatography is changed, 0.13mol/L NaCl elutions are first used, 0.3mol/L NaCl elutions is then used, collects 0.3mol/L NaCl and wash
De- component, obtains crude krill glycoprotein fraction, then passes through Sephadex G-100 sephadex columns after purification, reuses
High performance liquid chromatography is further purified, and chromatographic column is Inertsil ODS-3C18Column, mobile phase are acetonitrile and water (0.1% trifluoro
Acetic acid, flow velocity 1.5mL/min), obtain krill glycoprotein.
Embodiment 5
The present embodiment prepares ginseng beverage by following steps, including 4 parts of ginseng, 2 parts of krill glycoprotein, shark sugar
2 parts of albumen, 1 part of polydextrose, 240 parts of water；
(1) krill glycoprotein aqueous solution and shark is respectively prepared after enzymolysis and extraction in krill glycoprotein and shark glycoprotein
Fish glycoprotein aqueous solution；
(2) ginseng crushing, distillation are obtained into powder, krill glycoprotein aqueous solution and shark glycoprotein aqueous solution is added, and
Polydextrose mixing is added afterwards, 100 DEG C of digestion 18min obtain ginseng beverage after 26min is sterilized.
The wherein preparation method (wherein the preparation method of shark glycoprotein is identical as krill glycoprotein) of krill glycoprotein, packet
Include following steps：
(1) krill meat is homogenized in ethanol, adjusts pH value to 7.5 with the sodium hydroxide solution of 1mol/L, then uses water
It adjusts concentration of alcohol to 40%, 15min-25min is kept the temperature in 25 DEG C of -35 DEG C of water-baths, supernatant is collected by centrifugation for the first time, continues to use
Sodium chloride adjusts concentration and centrifuges 8min for the second time to 13%, 5000r/min, collects precipitation；
(2) obtained precipitation is handled into 2h using ether, filtering freeze-drying obtains degreasing protein powder；
(3) it is 1 according to solid-liquid ratio by obtained degreasing protein powder:60 is soluble in water, adjusts pH value to 5, pawpaw is added
Protease is digested, and 4h is digested in 48 DEG C, and the product destroy the enzyme treatment after enzymolysis obtains enzymolysis liquid, enzymolysis liquid is centrifuged to obtain
Clear liquid obtains krill glycoprotein crude product by processing；
(4) the krill glycoprotein crude product that step (3) obtains is soluble in water, is handed over by DEAE-cellulose-52 anion
Resin chromatography is changed, 0.18mol/L NaCl elutions are first used, 0.4mol/L NaCl elutions is then used, collects 0.4mol/L NaCl and wash
De- component, obtains crude krill glycoprotein fraction, then passes through Sephadex G-100 sephadex columns after purification, reuses
High performance liquid chromatography is further purified, and chromatographic column is Inertsil ODS-3C18Column, mobile phase are acetonitrile and water (0.1% trifluoro
Acetic acid, flow velocity 1.5mL/min), obtain krill glycoprotein.
Comparative example 1
Difference lies in do not contain krill glycoprotein, remaining component, component to this comparative example with embodiment 1 in this comparative example
Parts by weight and preparation method it is same as Example 1, ginseng beverage is prepared.
Comparative example 2
This comparative example and embodiment 1 difference lies in, the content of krill glycoprotein is 5 parts in this comparative example, remaining component,
The parts by weight and preparation method of component are same as Example 1, and ginseng beverage is prepared.
Comparative example 3
Difference lies in the content of krill glycoprotein is 0.5 part to this comparative example in this comparative example, remaining group with embodiment 1
Point, the parts by weight of component and preparation method it is same as Example 1, ginseng beverage is prepared.
Comparative example 4
Difference lies in the content of ginseng is 0.8 part to this comparative example in this comparative example, remaining component, component with embodiment 1
Parts by weight and preparation method it is same as Example 1, ginseng beverage is prepared.
Comparative example 5
Difference lies in the content of ginseng is 11 parts to this comparative example in this comparative example, remaining component, component with embodiment 1
Parts by weight and preparation method it is same as Example 1, ginseng beverage is prepared.
Comparative example 6
This comparative example and embodiment 1 difference lies in, enzyme used is flavor protease when being digested in this comparative example,
Krill glycoprotein is prepared, remaining component, the parts by weight of component and preparation method are same as Example 1, and ginseng drink is prepared
Material.
Comparative example 7
This comparative example and embodiment 3 difference lies in, the content of shark glycoprotein is 5 parts in this comparative example, remaining component,
The parts by weight and preparation method of component are same as Example 3, and ginseng beverage is prepared.
Comparative example 8
Difference lies in the content of shark glycoprotein is 0.5 part to this comparative example in this comparative example, remaining group with embodiment 3
Point, the parts by weight of component and preparation method it is same as Example 3, ginseng beverage is prepared.
Comparative example 9
Difference lies in do not contain polydextrose, remaining component, component in this comparative example for this comparative example and embodiment 1
Parts by weight and preparation method are same as Example 1, and ginseng beverage is prepared.
Embodiment 1-5 and comparative example the 1-9 ginseng beverage prepared are subjected to mouth feel score and the test that refreshes the mind (random choosing
Select 100 people to give a mark to this 14 kinds of beverages, then be averaged, wherein most preferably 100, it is minimum 0) and tumour cell
Growth inhibition ratio is tested, and the concrete outcome of test is as shown in table 1 below：
Wherein growth of tumour cell inhibiting rate test uses following methods：
Assay method refers to Wang Bin, Ren Shuwen, Li Guoqiang, and " the antitumor steroidal of Chinese tamarisk and flavone compound are ground Guan Huashi
Study carefully " it [is recorded in《Chinese Pharmaceutical Journal》, the 4th phase of volume 44 in 2009：The 576-580 pages], certain amount is in by when measurement
The 90 μ L of cell per well of exponential phase are inoculated in 96 hole microtest plates, are added per hole after culture for 24 hours and are waited for 10 μ L of test sample, often
A cell strain, each concentration are 3 multiple holes.Cell is in 37 DEG C, 5%CO2Under the conditions of after culture 48h, per Kong Jiayong physiology salts
The 20 μ L of MTT liquid for the 5gL-1 that water is prepared；Continue after cultivating 4h, three liquid (10%SDS-5% isobutanols-are added per hole
0.01molL-1HCl) 50 μ L, in CO2In incubator overnight.Then microplate reader is used to be surveyed per hole A in most suitable wavelength 570nm
Value.Inhibiting rate is calculated according to the following formula：Growth inhibition ratio IR (%)=(1-A medications group/A control groups) × 100%.Cell used
For human leukemia cell line P388.
Table 1
The content of the ginseng beverage provided by the invention it can be seen from the result in upper table, ginseng affects the mouth of beverage
Sense, by comparative example 4 and comparative example 5 with, if additive amount is excessive or very few, the mouthfeel of beverage has it can be seen from embodiment comparison
Larger decline.
And the effect that refreshes the mind of ginseng beverage, the effect that people participates in krill glycoprotein concentration compounding is depended on, especially
When ginseng concentration is not in the range of restriction, the effect to refresh the mind is excessively poor, this can be seen that by comparative example 4-5；And by right
For ratio 1 as can be seen that when not adding krill glycoprotein in beverage, the effect that refreshes the mind of ginseng beverage is excessively poor, illustrates
The addition of krill glycoprotein can promote ginseng further to play the health-care effect to refresh the mind, reach antifatigue and other effects.
Further, beverage also has the effect of antitumor, and this is mainly due to people to participate in krill glycoprotein, shark sugar egg
White collaboration plays the effect generated, when containing krill glycoprotein in beverage, has the apparent effect for inhibiting tumour cell production
Fruit further adds shark glycoprotein, can promote inhibition；When additive amount is not in this hair it can be seen from comparative example 2-3
When in the range of bright restriction, the inhibition of beverage is greatly reduced；And the krill for using other protease to be digested
When glycoprotein, the antitumor inhibiting rate of beverage prepared is declined, and illustrates that the enzyme that the present invention uses is specific；Comparative example 7-
8 with embodiment 3 comparison as can be seen that shark glycoprotein additive amount not in the framework of the present definition when, beverage it is antitumor
Effect is declined.
The addition of polydextrose has been obviously improved the product of ginseng beverage it can be seen from the comparison of embodiment 1 and comparative example 9
Matter improves the mouthfeel of beverage.
To sum up, ginseng beverage provided by the invention has good mouthfeel, antitumous effect and health-care effect, is suitable for
Masses drink and the health products as clinical auxiliary treatment use.
Applicant states that the present invention illustrates ginseng beverage of the invention and preparation method thereof by above-described embodiment and uses
On the way, but the invention is not limited in above-mentioned method detaileds, that is, it is real not mean that the present invention has to rely on above-mentioned method detailed ability
It applies.Person of ordinary skill in the field is it will be clearly understood that any improvement in the present invention, to the equivalent of each raw material of product of the present invention
Replacement and the addition of auxiliary element, the selection etc. of concrete mode, all fall within protection scope of the present invention and the open scope.
Claims (10)
1. a kind of ginseng beverage, which is characterized in that the ginseng beverage includes 2-8 parts of ginseng by weight；Krill glycoprotein
1-3 parts；0.5-2 parts of polydextrose；240-280 parts of water.
2. ginseng beverage according to claim 1, which is characterized in that the ginseng beverage includes ginseng 3- by weight
5 parts；2-3 parts of krill glycoprotein；1-2 parts of polydextrose；250-270 parts of water.
3. ginseng beverage according to claim 1 or 2, which is characterized in that the ginseng beverage further includes shark glycoprotein
1-3 parts.
4. ginseng beverage according to any one of claim 1-3, which is characterized in that the krill glycoprotein is passed through by krill
Enzymolysis and extraction is crossed to be prepared；
Preferably, the method for the enzymolysis and extraction krill glycoprotein includes the following steps：
(1) krill meat is homogenized in a solvent, adjusts pH value and homogenate concentrations, supernatant is collected by centrifugation for the first time, continues to use salt
Concentration is adjusted, precipitation is collected by centrifugation for the second time；
(2) precipitation that step (1) obtains is handled using solvent, filtering freeze-drying obtains degreasing protein powder；
(3) the degreasing protein powder obtained step (2) is soluble in water, adjusts pH value, and enzyme is added and is digested, the production after enzymolysis
Object destroy the enzyme treatment obtains enzymolysis liquid, and enzymolysis liquid is centrifuged to obtain supernatant, and krill glycoprotein crude product is obtained by processing；
(4) the krill glycoprotein crude product that step (3) obtains is soluble in water, and crude krill glycoprotein fraction is obtained by column chromatography,
Then pass through sephadex column after purification, reuses high performance liquid chromatography and be further purified, obtain the krill glycoprotein.
5. ginseng beverage according to claim 4, which is characterized in that solvent described in step (1) is ethyl alcohol；
Preferably, a concentration of 75%-85% of the ethyl alcohol；
Preferably, the reagent that pH value is adjusted described in step (1) is sodium hydroxide；
Preferably, it is to be adjusted to 7.1-8.8 that pH value is adjusted described in step (1)；
Preferably, it is to use water tune concentration of alcohol to 35%-42% that homogenate concentrations are adjusted described in step (1)；
Preferably, further include keeping the temperature 15min-25min in 25 DEG C of -35 DEG C of water-baths before being centrifuged for the first time described in step (1)；
Preferably, salt described in step (1) is sodium chloride；
Preferably, the sodium chloride adjusts the concentration of supernatant to 10%-15%；
Preferably, further include 10 DEG C of -20 DEG C of water-baths heat preservation 6h-9h after salt described in step (1) adjusts concentration；
Preferably, the rotating speed of second of centrifugation described in step (1) is 3000r/min-5000r/min；
Preferably, the time of second of centrifugation described in step (1) is 4min-10min；
Preferably, solvent described in step (2) is ether；
Preferably, the mass ratio of defatted protein powder and water described in step (3) is 1:(42-66)；
Preferably, the reagent that pH value is adjusted described in step (3) is hydrochloric acid；
Preferably, it is to be adjusted to 3-5.5 that pH value is adjusted described in step (3)；
Preferably, enzyme described in step (3) is papain；
Preferably, the enzyme activity of the papain is more than 2.5 × 106U/g；
Preferably, the quality of enzyme described in step (3) is the 0.5%-0.7% of degreasing protein sub-prime amount；
Preferably, the time digested described in step (3) is 2h-4h；
Preferably, the temperature digested described in step (3) is 36 DEG C -50 DEG C；
Preferably, column chromatography described in step (4) chromatographs for anion exchange resin；
Preferably, the anion exchange resin is DEAE-cellulose-52；
Preferably, the preparation method of the shark glycoprotein is identical as the preparation method of krill glycoprotein.
6. ginseng beverage according to any one of claims 1-5, which is characterized in that the ginseng beverage includes ginseng 3-
8 parts；2-3 parts of krill glycoprotein；2-3 parts of shark glycoprotein；1-2 parts of polydextrose；240-280 parts of water.
7. the preparation method of the ginseng beverage according to any one of claim 1-6, which is characterized in that the method includes
Following steps：Ginseng, krill glycoprotein, polydextrose and water and optionally shark glycoprotein mixing, digestion, sterilization are obtained
The ginseng beverage.
8. the method according to the description of claim 7 is characterized in that the described method comprises the following steps：
(a) by krill glycoprotein and shark glycoprotein after enzymolysis and extraction, krill glycoprotein aqueous solution and shark sugar is respectively prepared
Protein solution；
(b) ginseng crushing, distillation are obtained into powder, krill glycoprotein aqueous solution and shark glycoprotein aqueous solution is added, then added
Enter polydextrose mixing, digestion obtains the ginseng beverage after sterilization.
9. according to the method described in claim 8, it is characterized in that, concentration of aqueous solution described in step (a) is 0.5mg/L-
5mg/L；
Preferably, the temperature of digestion described in step (b) is 80 DEG C -100 DEG C；
Preferably, the time of digestion described in step (b) is 16min-18min；
Preferably, the method sterilized described in step (b) is that ultrasonic sterilization or electromagnetic field are sterilized；
Preferably, the time sterilized described in step (b) is 26min-68min.
10. the ginseng beverage according to any one of claim 1-6 is preparing anti-fatigue health-product containing or nourishment for vitality health care
Purposes in product.
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| CN201810133872.3A CN108271980A (en) | 2018-02-09 | 2018-02-09 | A kind of ginseng beverage and its preparation method and application |
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| CN201810133872.3A CN108271980A (en) | 2018-02-09 | 2018-02-09 | A kind of ginseng beverage and its preparation method and application |
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| CN108271980A true CN108271980A (en) | 2018-07-13 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109043260A (en) * | 2018-08-03 | 2018-12-21 | 福建省力菲克药业有限公司 | One seed ginseng class beverage and preparation method thereof |
| CN114451505A (en) * | 2022-02-21 | 2022-05-10 | 福建农林大学 | Anti-fatigue beverage added with glycoprotein |
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| CN103113462A (en) * | 2011-11-16 | 2013-05-22 | 浙江海洋学院 | Shark glycoprotein, and preparation method and application thereof |
| CN106942538A (en) * | 2017-03-28 | 2017-07-14 | 山东华胶饮品有限公司 | Formula of donkey-hide gelatin ginseng juice's composite beverage and preparation method thereof |
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| CN103113462A (en) * | 2011-11-16 | 2013-05-22 | 浙江海洋学院 | Shark glycoprotein, and preparation method and application thereof |
| CN106942538A (en) * | 2017-03-28 | 2017-07-14 | 山东华胶饮品有限公司 | Formula of donkey-hide gelatin ginseng juice's composite beverage and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109043260A (en) * | 2018-08-03 | 2018-12-21 | 福建省力菲克药业有限公司 | One seed ginseng class beverage and preparation method thereof |
| CN114451505A (en) * | 2022-02-21 | 2022-05-10 | 福建农林大学 | Anti-fatigue beverage added with glycoprotein |
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