CN108208811A - A kind of preparation method of Cordyceps militaris pectase - Google Patents
A kind of preparation method of Cordyceps militaris pectase Download PDFInfo
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- CN108208811A CN108208811A CN201810085470.0A CN201810085470A CN108208811A CN 108208811 A CN108208811 A CN 108208811A CN 201810085470 A CN201810085470 A CN 201810085470A CN 108208811 A CN108208811 A CN 108208811A
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- cordyceps militaris
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- 239000000047 product Substances 0.000 description 9
- KQLDDLUWUFBQHP-UHFFFAOYSA-N Cordycepin Natural products C1=NC=2C(N)=NC=NC=2N1C1OCC(CO)C1O KQLDDLUWUFBQHP-UHFFFAOYSA-N 0.000 description 5
- OFEZSBMBBKLLBJ-BAJZRUMYSA-N cordycepin Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)C[C@H]1O OFEZSBMBBKLLBJ-BAJZRUMYSA-N 0.000 description 5
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- 239000004472 Lysine Substances 0.000 description 1
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- 206010057249 Phagocytosis Diseases 0.000 description 1
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- 241000382353 Pupa Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
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- 239000011707 mineral Substances 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
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- 150000002772 monosaccharides Chemical class 0.000 description 1
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- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
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- 102000004169 proteins and genes Human genes 0.000 description 1
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- CDBYLPFSWZWCQE-UHFFFAOYSA-L sodium carbonate Substances [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of preparation method of Cordyceps militaris pectase, this method includes the following steps：A) fruiting bodies of cordyceps militaris crushes, and adds in purified water, adds in chitinase, and temperature is 55~65 DEG C, and pH is 5.5~6.6, digests 4~6h, then enzyme deactivation, filtering, concentration；B) a variety of fruits and vegetables are cleaned, ultraviolet disinfection, is sliced, squeezes the juice, add in maltitol and xylitol；C) juice is mixed to get Cordyceps militaris juice in proportion with Cordyceps militaris concentrate；D) seven kinds of probiotic powders are respectively accessed in Cordyceps militaris juice in proportion for bifidobacterium adolescentis, Lactobacillus casei, lactobacillus acidophilus, streptococcus thermophilus, Lactobacillus rhamnosus, lactobacillus plantarum and lactobacillus bulgaricus, standing for fermentation.Cordyceps militaris pectase stable quality of the present invention, sweet mouthfeel is moderate, and Cordyceps sinensis polysaccharide content is high and product stability is good, has the function of strengthen immunity.
Description
Technical field
The invention belongs to field of fermentation engineering, and in particular to a kind of preparation method of Cordyceps militaris pectase.
Background technology
Ferment is also known as enzyme, is the catalyst of various biological respinses, is a kind of protein with sp act, it exists
In each physiological response system and organ-tissue, use catalysis and regulation and control in vivo all biochemical reactions and metabolism into
Row.Being metabolized of every cell in human body, it is newborn, decompose, digestion, synthesis etc., depend on ferment to complete, can more determine ours
The length in service life is with short, therefore ferment is referred to as " Source of life " again.Ferment also has " specificity ", each ferment can only urge
Change a kind of biochemical reaction to carry out, therefore the ferment type in human body is up to thousands of kinds.Ferment is probiotic action in various food materials,
The easy absorbing material of various small molecules that food materials are resolved into, such as various monosaccharide, amino acid, vitamin, minerals etc. easily absorb
Small-molecule substance.
Ferment is usually using fruits and vegetables as raw material, by adding different probiotics, at most few then some months, several years, process
Complex process, the time is long, and easy living contaminants, product quality is difficult to control.
Cordyceps militaris is also known as northern Chinese caterpillar Fungus, northeast cordyceps sinensis etc., and medicinal material is belonged to cordyceps sinensis.It sends out under study for action in recent years
Existing, Cordyceps militaris has important pharmaceutical value, has and improves immune function, and protect liver clearing lung-heat is relieving cough and reducing sputum, antifatigue, anti anoxia,
It is antiviral and other effects.Since the success of Cordyceps militaris artificial cultivation, people to the cultivation technique of Cordyceps militaris, grind by pharmacology and toxicity etc.
Study carefully very extensively.Research shows that Cordyceps militaris has the function of extremely similar to cordyceps sinensis, have no toxic side effect.It can be as cordyceps sinensis
Substitute.
The Cordyceps militaris of existing Cordyceps militaris pectase product addition mostly extracts cordycepin using organic solvent, and extraction process is multiple
It is miscellaneous and easily cause chemical contamination, and content of functional component is low, effect unobvious；In addition, existing Cordyceps militaris pectase production
Product add white granulated sugar or sucrose as carbon source through fermentation more, easily cause blood glucose rise, are not suitable for diabetes patient.
Invention content
The purpose of the present invention is above-mentioned shortcoming is overcome to provide, a kind of stability is good, and sweet mouthfeel is moderate, Cordyceps sinensis polysaccharide
Content is high and diabetes patient is suitble to have the function of the preparation method of the Cordyceps militaris pectase of strengthen immunity.It and should
Method extraction process is simple, environmentally protective.
The purpose of the present invention is what is be accomplished by the following way：
A kind of preparation method of Cordyceps militaris pectase, this method include the following steps：
A) fruiting bodies of cordyceps militaris crushes, addition purified water, and the weight ratio of Cordyceps militaris and purified water is 1:10~15, then add
Enter chitinase, enzyme concentration is 1%~2%, and temperature is 55~65 DEG C, under the conditions of pH is 5.5~6.5, digests 4~6h, then
Enzyme deactivation filters, is concentrated to give Cordyceps militaris concentrate；
B) a variety of fruits and vegetables are cleaned, sterilized, slice is squeezed the juice, and adds in maltitol and xylitol obtains juice；
C) juice and Cordyceps militaris concentrate are pressed 3~5：1 weight ratio is mixed to get Cordyceps militaris juice, adjusts pH4
~6；
D) compound probiotic powder is accessed in Cordyceps militaris juice, 37~42 DEG C are left to ferment 4~6 days, pasteurize.
The step a) is to carry out in a kettle, and when enzymolysis need to be stirred continuously or be spaced stirring, after enzymolysis, anti-
Kettle temperature degree is answered to rise to 100 DEG C or more and carries out enzyme deactivation, 20~30min of enzyme deactivation time.
To be concentrated in vacuo, condition is 0.075~0.09MPa of vacuum degree for concentration described in step a), and temperature is 55~65 DEG C,
It is 1~1.05 to be concentrated into density, obtains Cordyceps militaris concentrate.
In step b), Kiwi berry, mulberry fruit, blueberry, grape, tomato and the celery of the weight such as a variety of fruits and vegetables are.
In step b), the dosage of maltitol is the 8%~10% of fruits and vegetables weight, and the dosage of xylitol is fruits and vegetables weight
8%~10%.
In step b), preferably the dosage of maltitol is the 10% of fruits and vegetables total weight, and the dosage of xylitol is fruits and vegetables gross weight
The 10% of amount.
Disinfection described in step b) is using ultraviolet disinfection, and disinfecting time is 25~35min；The slice is to be cut into
The thin slice of 3~5mm thickness.
After maltitol and xylitol are added in step b), then with ultraviolet disinfection handle 30min.
In step d), the compound probiotic powder is bifidobacterium adolescentis, Lactobacillus casei, lactobacillus acidophilus, thermophilic
Streptococcus, Lactobacillus rhamnosus, lactobacillus plantarum and lactobacillus bulgaricus；Bifidobacterium adolescentis, Lactobacillus casei, acidophilus breast
Bacillus, streptococcus thermophilus, Lactobacillus rhamnosus, lactobacillus plantarum and lactobacillus bulgaricus respectively access pupa in 0.5~1% ratio
In cordyceps sinensis juice, seven total inoculum concentration is 3.5~7%.
Beneficial effects of the present invention compared with the prior art：
(1) this product is combined Cordyceps militaris with fruits and vegetables, there is significant strengthen immunity；
(2) Cordyceps militaris cell wall is crushed by adding chitinase, cellular content functional component is made to be easier to discharge,
The content of active ingredient can be improved, reduces technological process and the use of organic solvent again relative to water extract-alcohol precipitation, extracts work
Skill is simple and reduces chemical contamination.
(3) ferment prepared by the method for the present invention can be sufficiently reserved cordycepin abundant in Cordyceps militaris, improve easy miscellaneous bacteria
The situation of pollution can effectively inhibit the growth of miscellaneous bacteria, can make shelf life of products up to 18 months or more without adding preservative.
(4) this product substitutes traditional white granulated sugar or glucose with glycitols substance, can promote the growth and breeding of probiotics,
Accelerate course of fermentation, can terminate within 4~6 days to ferment；And blood glucose rise will not be caused, it is particularly suitable for diabetes patient.
Specific embodiment：
It is further illustrated the present invention below by way of specific embodiment.But the detail of embodiment is only used for explaining this hair
It is bright, it should not be construed as limited overall technical solution.
Embodiment 1
1st, the preparation of Cordyceps militaris juice
Fruiting bodies of cordyceps militaris dried food and nuts is crushed to obtain fruiting bodies of cordyceps militaris powder in high speed disintegrator, in a kettle
The weight ratio of addition purified water, Cordyceps militaris and purified water is 1:10, chitinase is added in, carries out broken wall treatment, enzyme concentration is
1.5%, temperature is 55 DEG C, pH 5.5, digests 5h, and when enzymolysis need to be stirred continuously or be spaced stirring, need to be anti-after enzymolysis
Kettle temperature degree is answered to rise to 100 DEG C or more, then enzyme deactivation 20min carries out centrifugal filtration, filtrate is concentrated in vacuo, and condition is true
Reciprocal of duty cycle 0.075MPa, temperature are 60 DEG C, and it is 1.05 to be concentrated into 60 DEG C of density, obtains Cordyceps militaris concentrate.
Fruits and vegetables：Kiwi berry, mulberry fruit, blueberry, grape, tomato and each 1 parts by weight of celery, are cleaned with purified water,
It drains away the water, ultraviolet disinfection about 30min is cut into the thin slice of 3~5mm, is smashed to pieces, squeezed the juice with high-speed homogenization machine, adds in fruits and vegetables weight
10% maltitol, fruits and vegetables weigh 10% xylitol, then handle 30min with ultraviolet disinfection.
2nd, the preparation of Cordyceps militaris pectase
Juice presses 4 with Cordyceps militaris concentrate:1 weight ratio is mixed to get Cordyceps militaris juice, then adds citric acid
Adjust pH to 6.0.It is bifidobacterium adolescentis, Lactobacillus casei, lactobacillus acidophilus, streptococcus thermophilus, sandlwood by seven kinds of probiotic powders
Sugared lactobacillus, lactobacillus plantarum and lactobacillus bulgaricus are respectively accessed in 1% ratio in Cordyceps militaris juice, total inoculum concentration 7%,
It 37 DEG C, is left to ferment 5 days.
3rd, finished product detection and filling
There is the distinctive fragrance of Cordyceps militaris through the Cordyceps militaris pectase obtained by everfermentation, the soft lubrication of entrance is sour-sweet suitable
In.By 100 DEG C, 10min of pasteurize, cooling is filled into bottle.
In the Cordyceps militaris pectase that the present embodiment is prepared, for soluble solid up to 20.1%, cordycepin content can
Up to 0.23%, Cordyceps sinensis polysaccharide content is up to 2.6%；18 months pectase room temperature lower shelf-lifves, pectase stable and uniform.
Embodiment 2
1st, the preparation of Cordyceps militaris juice
Fruiting bodies of cordyceps militaris dried food and nuts is crushed to obtain fruiting bodies of cordyceps militaris powder in high speed disintegrator, in a kettle
The weight ratio of addition purified water, Cordyceps militaris and purified water is 1:15, add in chitinase, enzyme concentration 2%, temperature 60
DEG C, pH 6 digests 5h, and when enzymolysis need to be stirred continuously or be spaced stirring, after enzymolysis, temperature of reaction kettle need to be risen to 100
DEG C or more, then enzyme deactivation 30min carries out centrifugal filtration, filtrate is concentrated in vacuo, and condition is vacuum degree 0.08MPa, and temperature is
65 DEG C, it is 1.05 to be concentrated into density, obtains Cordyceps militaris concentrate.
Fruits and vegetables：Kiwi berry, mulberry fruit, blueberry, grape, tomato and each 1 parts by weight of celery, are cleaned with purified water,
It drains away the water, ultraviolet disinfection about 30min is cut into the thin slice of 3~5mm, is smashed to pieces with high-speed homogenization machine, adds in the wheat that fruits and vegetables weigh 8%
Bud sugar alcohol, fruits and vegetables weigh 8% xylitol, then handle 30min with ultraviolet disinfection.
2nd, the preparation of Cordyceps militaris pectase
Juice presses 4 with Cordyceps militaris concentrate:1 weight ratio is mixed to get Cordyceps militaris juice, then adds citric acid
Adjust pH to 6.0.It is bifidobacterium adolescentis, Lactobacillus casei, lactobacillus acidophilus, streptococcus thermophilus, sandlwood by seven kinds of probiotic powders
Sugared lactobacillus, lactobacillus plantarum and lactobacillus bulgaricus are respectively accessed in 0.8% ratio in Cordyceps militaris juice, total inoculum concentration
5.6%, it 37 DEG C, is left to ferment 5 days.
3rd, finished product detection and filling
There is the distinctive fragrance of Cordyceps militaris through the Cordyceps militaris pectase obtained by everfermentation, the soft lubrication of entrance is sour-sweet suitable
In.By 100 DEG C, 10min of pasteurize, cooling is filled into bottle, through being Cordyceps militaris pectase after the assay was approved.
For soluble solid up to 20.3%, cordycepin content is reachable in the Cordyceps militaris pectase that the present embodiment is prepared
0.35%, Cordyceps sinensis polysaccharide content is up to 2.8%；18 months pectase room temperature lower shelf-lifves, pectase stable and uniform.
Embodiment 3
1st, the preparation of Cordyceps militaris juice
Fruiting bodies of cordyceps militaris dried food and nuts is crushed to obtain fruiting bodies of cordyceps militaris powder in high speed disintegrator, in a kettle
The weight ratio of addition purified water, Cordyceps militaris and purified water is 1:10, add in chitinase, enzyme concentration 1%, temperature 55
DEG C, pH 6 digests 6h, and when enzymolysis need to be stirred continuously or be spaced stirring, after enzymolysis, temperature of reaction kettle need to be risen to 100
DEG C or more, then enzyme deactivation 25min carries out centrifugal filtration, filtrate is concentrated in vacuo, condition be vacuum degree 0.075MPa, temperature
It it is 65 DEG C, it is 1.05 to be concentrated into density, obtains Cordyceps militaris concentrate.
Fruits and vegetables：Kiwi berry, mulberry fruit, blueberry, grape, tomato and each 1 parts by weight of celery, are cleaned with purified water,
It drains away the water, ultraviolet disinfection about 30min is cut into the thin slice of 3~5mm, is smashed to pieces with high-speed homogenization machine, adds in the wheat that fruits and vegetables weigh 9%
Bud sugar alcohol, fruits and vegetables weigh 9% xylitol, then handle 30min with ultraviolet disinfection.
2nd, the preparation of Cordyceps militaris fruits and vegetables fermentation ferment
Juice presses 5 with Cordyceps militaris concentrate:1 weight ratio is mixed to get Cordyceps militaris juice, then adds citric acid
Adjust pH to 6.0.It is bifidobacterium adolescentis, Lactobacillus casei, lactobacillus acidophilus, streptococcus thermophilus, sandlwood by seven kinds of probiotic powders
Sugared lactobacillus, lactobacillus plantarum and lactobacillus bulgaricus are respectively accessed in 1% ratio in Cordyceps militaris juice, total inoculum concentration 7%,
It 37 DEG C, is left to ferment 5 days.
3rd, finished product detection and filling
There is the distinctive fragrance of Cordyceps militaris through the Cordyceps militaris pectase obtained by everfermentation, the soft lubrication of entrance is sour-sweet suitable
In.By 100 DEG C, 10min of pasteurize, cooling is filled into bottle, through being Cordyceps militaris pectase after the assay was approved.
For soluble solid up to 20.2%, cordycepin content is reachable in the Cordyceps militaris pectase that the present embodiment is prepared
0.29%, Cordyceps sinensis polysaccharide content is up to 2.5%；18 months pectase room temperature lower shelf-lifves, pectase stable and uniform.
Below by specific example, the invention will be further described：
Small white mouse 60, male female fifty-fifty, weight is close.It is randomly divided into 3 groups, every group 20, I group of control group；II present invention
1 Cordyceps militaris pectase group of embodiment, III Cordyceps militaris concentrates group (are prepared) according to step described in embodiment 1, I group of difference
10mL normal diets (formula is fed daily：Silkworm chrysalis 3%, blood meal 3%, soya bean 13%, fish meal 5%, lysine 0.25%, egg ammonia
Acid 0.4%, potassium dihydrogen phosphate 1%, mixed vitamin 1%, remaining is basic feed)；II group is fed 10mL normal diets daily,
1 times/day of 10mL Cordyceps militaris pectase is fed simultaneously, and each 10mL (10ml/ bottles) is fed 1 month.III groups are fed daily
10mL normal diets, while 1 times/day, each 10mL of 10mL Cordyceps militaris concentrate is fed, it feeds 1 month.
The measure of mouse immune shoot formation：Mouse is put to death after experiment and is weighed, then takes out spleen, thymus gland
It weighs respectively, and calculates the ratio of internal organs/weight.
Phagocytic function measures：Using clearance in mice measuring.Mouse is dilute by 4 times of 10g/0.1mL tail veins injection
The india ink released takes 30 μ L of blood from posterior orbit when 3min, 11min, is added to 3mL 0.1%Na2CO3Solution shakes up,
OD values are surveyed at 640nm wavelength, while make blank control, calculate phagocytic index.
Chicken red blood cell (CRBC) is tested：At abdominal cavity mouse, then, abdomen are put to death after CRBC the suspensions 0.5mL, lh of injection 5%
Chamber injects 2mL physiological saline, and soft mouse web portion takes peritoneal fluid film-making, 37 DEG C of incubation 0.5h of plate is put into, then with life
Manage normal saline washing, drying, acetone：Methanol solution (1：1) fixed 5min, Giemsa stain (4%) dye 3min, micro- sem observation meter
The macrophage quantity of chicken red blood cell is swallowed in 100 phagocytes of number, and calculates phagocytic percentage.
Specific experiment result data is shown in Tables 1 and 2：
Influence of the 1 Cordyceps militaris pectase of table to mouse immune organ
Influence of the 2 Cordyceps militaris pectase of table to mouse phagocyte imdex and phagocyte phagocytic function
By Tables 1 and 2 as it can be seen that compared with the control group, Cordyceps militaris pectase can significantly improve Turnover of Mouse Peritoneal Macrophages
Phagocytosis to CRBC, significant difference (P ＜ 0.01).Show that Cordyceps militaris pectase can enhance the macrophage of mouse and swallow thin
The phagocytic function of born of the same parents, while the also significant difference (P ＜ 0.05) compared with Cordyceps militaris concentrate group.Illustrate Cordyceps militaris of the present invention
Pectase has synergistic function, has the function of significant strengthen immunity.
Claims (10)
1. a kind of preparation method of Cordyceps militaris pectase, it is characterised in that this method includes the following steps：
A) fruiting bodies of cordyceps militaris crushes, addition purified water, and the weight ratio of Cordyceps militaris and purified water is 1:10~15, it adds several
Fourth matter enzyme, enzyme concentration 1%~2%, temperature are 55~65 DEG C, under the conditions of pH is 5.5~6.5, digest 4~6h, then enzyme deactivation, mistake
It filters, be concentrated to give Cordyceps militaris concentrate；
B) a variety of fruits and vegetables are cleaned, sterilized, slice is squeezed the juice, and adds in maltitol and xylitol obtains juice；
C) juice and Cordyceps militaris concentrate are pressed 3~5：1 weight ratio is mixed to get Cordyceps militaris juice, adjusts pH4~6；
D) compound probiotic is accessed in Cordyceps militaris juice, 37~42 DEG C are left to ferment 4~6 days.
2. the preparation method of Cordyceps militaris pectase according to claim 1, it is characterised in that the step a) is anti-
It answers in kettle and carries out, when enzymolysis need to be stirred continuously or be spaced stirring, and after enzymolysis, temperature of reaction kettle is risen to 100 DEG C or more
Enzyme deactivation is carried out, the enzyme deactivation time is 20~30min.
3. the preparation method of Cordyceps militaris pectase according to claim 1, it is characterised in that the concentration described in step a)
To be concentrated in vacuo, condition is 0.075~0.09MPa of vacuum degree, and temperature is 55~65 DEG C, and it is 1~1.05 to be concentrated into density, is obtained
Cordyceps militaris concentrate.
4. the preparation method of Cordyceps militaris pectase according to claim 1, it is characterised in that it is more in the step b)
Kind of fruits and vegetables for etc. weight Kiwi berry, mulberry fruit, blueberry, grape, tomato and celery.
5. the preparation method of Cordyceps militaris pectase according to claim 1, it is characterised in that in the step b), malt
The dosage of sugar alcohol is the 8%~10% of fruits and vegetables weight, and the dosage of xylitol is the 8%~10% of fruits and vegetables weight.
6. the preparation method of Cordyceps militaris pectase according to claim 5, it is characterised in that in the step b), malt
The dosage of sugar alcohol is the 10% of fruits and vegetables total weight, and the dosage of xylitol is the 10% of fruits and vegetables total weight.
7. the preparation method of Cordyceps militaris pectase according to claim 1, it is characterised in that described in the step b)
Disinfection be using ultraviolet disinfection, disinfecting time is 25~35min；The slice is the thin slice for being cut into 3~5mm thickness.
8. the preparation method of Cordyceps militaris pectase according to claim 1, it is characterised in that added in the step b)
After maltitol and xylitol, then with ultraviolet disinfection handle 30min.
9. the preparation method of Cordyceps militaris pectase according to claim 1, it is characterised in that in step d), described answers
Conjunction probiotics is bifidobacterium adolescentis, Lactobacillus casei, lactobacillus acidophilus, streptococcus thermophilus, Lactobacillus rhamnosus, plant breast bar
Bacterium and lactobacillus bulgaricus.
10. the preparation method of Cordyceps militaris pectase according to claim 9, it is characterised in that bifidobacterium adolescentis is done
Lactobacillus paracasei, lactobacillus acidophilus, streptococcus thermophilus, Lactobacillus rhamnosus, lactobacillus plantarum and lactobacillus bulgaricus respectively press 0.5
In~1% ratio access Cordyceps militaris juice, seven total inoculum concentration is 3.5~7%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109717340A (en) * | 2019-03-01 | 2019-05-07 | 江南大学 | A kind of fermentation preparation of the two-step Cordyceps militaris ferment of combination complex enzyme hydrolysis |
| CN111528427A (en) * | 2020-05-09 | 2020-08-14 | 青岛国海生物制药有限公司 | Laiyang pear enzyme paste added with cordyceps militaris and preparation method of Laiyang pear enzyme paste |
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| CN104856015A (en) * | 2015-04-24 | 2015-08-26 | 西藏月王生物技术有限公司 | A cordyceps sinensis enzyme and a preparation method thereof |
| CN105167090A (en) * | 2015-09-29 | 2015-12-23 | 江苏阜丰生物科技有限公司 | Hericium erinaceus, fruit and vegetable drink and production method thereof |
| CN107198074A (en) * | 2017-06-08 | 2017-09-26 | 河北中佳本草生物技术有限公司 | A kind of integration of drinking and medicinal herbs liquid ferment and preparation method thereof |
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| CN101780119A (en) * | 2010-03-22 | 2010-07-21 | 江门市鸿豪生物科技有限公司 | Cordyceps militaris and Agaricus blazei medicated wine and preparation method thereof |
| CN104856015A (en) * | 2015-04-24 | 2015-08-26 | 西藏月王生物技术有限公司 | A cordyceps sinensis enzyme and a preparation method thereof |
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| CN109717340A (en) * | 2019-03-01 | 2019-05-07 | 江南大学 | A kind of fermentation preparation of the two-step Cordyceps militaris ferment of combination complex enzyme hydrolysis |
| CN109717340B (en) * | 2019-03-01 | 2022-07-08 | 江南大学 | Fermentation preparation method of two-step cordyceps militaris enzyme combined with composite enzymolysis |
| CN111528427A (en) * | 2020-05-09 | 2020-08-14 | 青岛国海生物制药有限公司 | Laiyang pear enzyme paste added with cordyceps militaris and preparation method of Laiyang pear enzyme paste |
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Application publication date: 20180629 |