CN108184881A - A kind of missible oil containing copper rosinate and pyraclostrobin, preparation method and applications - Google Patents
A kind of missible oil containing copper rosinate and pyraclostrobin, preparation method and applications Download PDFInfo
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- CN108184881A CN108184881A CN201711461182.2A CN201711461182A CN108184881A CN 108184881 A CN108184881 A CN 108184881A CN 201711461182 A CN201711461182 A CN 201711461182A CN 108184881 A CN108184881 A CN 108184881A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/24—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing the groups, or; Thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
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Abstract
The invention discloses a kind of missible oil containing copper rosinate and pyraclostrobin, preparation method and applications, belong to technical field of pesticide.The missible oil containing copper rosinate and pyraclostrobin includes the raw material of following parts by weight：1 40 parts of 1 30 parts of copper rosinate and pyraclostrobin.The invention also discloses the preparation method and applications of the above-mentioned missible oil containing copper rosinate and pyraclostrobin.The present invention uses copper rosinate and pyraclostrobin that missible oil is made for raw material, can generate higher synergistic effect, expands broad-spectrum, overcomes and pathogen is delayed to develop immunity to drugs.Preventive effect is better than single dose, resistance of rainwater washing against, quick-acting is good, the lasting period is long, reduces access times, can prevent a variety of pathogens and disease simultaneously, reduce drug cost.Especially there is apparent synergistic effect to citrus red mite, citrus rust mite, citrus resin disease, anthracnose of orange, citrus downy mildew, citrus ulcer, citrus gray mold and citrus whitefly aphid, control effect is good.
Description
Technical field
The present invention relates to a kind of missible oil containing copper rosinate and pyraclostrobin, preparation method and applications, belong to pesticide
Technical field.
Background technology
Citrus psylla is unique medium that yellow twig is propagated, and in the case where there is tender tip in the same year, can occur 11~14 within 1 year
Generation, field generations overlapping, single wood louse pass sick rate and are up to 70-80%, and adult can fly, and difficulty of prevention and cure is high.It is meanwhile each in citrus
Growth period, in addition to citrus psylla, the also insect pests such as citrus red mite, citrus rust mite, citrus leaf-miner, citrus whitefly aphid
With citrus resin disease (rubber disease), anthracnose of orange, citrus downy mildew, citrus gray mold, citrus ulcer, alternaria of orange etc.
Occur while disease, so such pest and disease damage problem in the urgent need to address.
At present, due to the single use of pesticide and medication not science, unreasonable, pest has quickly been aggravated to currently used
The resistance of pesticide shortens some high costs, the service life for putting into the pesticide new component developed for a long time, while access times
It is more, it also aggravated environmental pollution, increased drug cost.At this stage for being agriculturally also easy to produce the disease control of resistance, mainly
Method is the compounding developed between the new component of existing kind no interactions resistance or existing pesticide species, the former is due to required
The speed that the development rate that development cost is high, the period is long and current is much generated less than pathogen drug resistance.
In consideration of it, there is an urgent need for developing a kind of new missible oil and preparation method thereof, to make up drawbacks described above.
Invention content
An object of the present invention is to provide a kind of missible oil containing copper rosinate and pyraclostrobin.The missible oil of the present invention,
Higher synergistic effect can be generated, expands broad-spectrum, overcomes and pathogen is delayed to develop immunity to drugs.Preventive effect is better than single dose, resistance to
Rain drop erosion, quick-acting is good, the lasting period is long, reduces access times, time saving, laborsaving, can prevent a variety of pathogens and disease simultaneously
Evil, reduces drug cost.Especially to citrus red mite, citrus rust mite, citrus resin disease, anthracnose of orange, citrus downy mildew
Disease, citrus ulcer, citrus gray mold and citrus whitefly aphid have apparent synergistic effect, and control effect is good.
The technical solution that the present invention solves above-mentioned technical problem is as follows：A kind of breast containing copper rosinate and pyraclostrobin
Oil includes the raw material of following parts by weight：1-40 parts of 1-30 parts of copper rosinate and pyraclostrobin.
Raw material brief introduction used in the present invention：
Copper rosinate is a kind of novel copper agent sterilization pesticide of high-efficiency low-toxicity wide spectrum, long, easy to use with the lasting period
New feature, overcome many shortcomings of Bordeaux mixture, be the preferable fungicide for replacing Bordeaux mixture, and have prevention ＆ protection and
Treat double action.Copper rosinate can be used for preventing a variety of fungies and the frequently seen plants disease caused by bacterium, have to vegetables bright
Aobvious stimulating growth effect, can replace, spray effect is good with other fungicide.Copper rosinate used in the present invention is commercially available purchase.
Pyraclostrobin is the methoxy acrylic bactericide for having both pyrrazole structure, can prevent by a variety of fungies
Disease caused by cause of disease.Pyraclostrobin used in the present invention is commercially available purchase.
In the prior art, citrus red mite, citrus rust mite, citrus resin disease (rubber disease), anthracnose of orange, citrus frost
Mildew, citrus ulcer, citrus gray mold and citrus whitefly aphid, produce pyraclostrobin certain drug resistance, individually
Using ideal control effect has been not achieved.The present invention uses copper rosinate and pyraclostrobin that missible oil is made for raw material, can produce
Raw higher synergistic effect, expands broad-spectrum, overcomes and pathogen is delayed to develop immunity to drugs.Preventive effect is better than single dose, resistance to rainwater
Wash away, quick-acting is good, the lasting period is long, reduce access times, it is time saving, laborsaving, a variety of pathogens and disease can be prevented simultaneously, drop
Low drug cost.Especially to citrus red mite, citrus rust mite, citrus resin disease, anthracnose of orange, citrus downy mildew, mandarin orange
Tangerine canker, citrus gray mold and citrus whitefly aphid have apparent synergistic effect, and control effect is good.
Based on the above technical solution, the present invention can also be improved as follows.
Further, include the raw material of following parts by weight：1-40 parts of 1-10 parts of copper rosinate and pyraclostrobin.
Further, include the raw material of following parts by weight：1-40 parts of 1-15 parts of copper rosinate and pyraclostrobin.
Further, include the raw material of following parts by weight：1-40 parts of 1-20 parts of copper rosinate and pyraclostrobin.
Further, include the raw material of following parts by weight：1-20 parts of 1-30 parts of copper rosinate and pyraclostrobin.
Further, include the raw material of following parts by weight：1-20 parts of 1-20 parts of copper rosinate and pyraclostrobin.
Further, include the raw material of following parts by weight：1-20 parts of 1-15 parts of copper rosinate and pyraclostrobin.
Further, include the raw material of following parts by weight：1-15 parts of 1-15 parts of copper rosinate and pyraclostrobin.
Further, include the raw material of following parts by weight：20 parts of 15 parts of copper rosinate and pyraclostrobin.
Further, the missible oil also contains auxiliary agent；The weight of the total weight and auxiliary agent of the copper rosinate and pyraclostrobin
The ratio of amount is 1：(0.8-1).
Further, the auxiliary agent includes dispersant, emulsifier, antifreezing agent, thickener, antifoaming agent, solvent, wetting agent, collapses
Solve the mixture of one or more of agent, filler.
Further, the mixture that the auxiliary agent is dispersant, emulsifier and solvent form；The copper rosinate and pyrazoles
The total weight of kresoxim-methyl and dispersant, emulsifier, solvent the ratio of weight be 1：(0.1-15)：(0.1-12)：(25.9-
95.8)。
Further, the dispersant is sodium lignin sulfonate, naphthalene sulfonic acid-formaldehyde condensation product sodium salt, alkylphenol-polyethenoxy
Ether formaldehyde condensation products sodium sulphate, alkyl phenol polyoxyethylene ether, sodium polycarboxylate, aliphatic amine polyoxyethylene ether and glycerin polyoxyethylene ether
The mixture of one or more of aliphatic ester.
Further, the emulsifier is alkyl phenol polyoxyethylene ether phosphate, polyoxyethylene aliphatic alcohol ether, styryl
Phenol polyoxyethylene ether, polyoxyethylene carboxylate sodium phosphate, calcium dodecyl benzene sulfonate, aliphatic acid polyethenoxy base ester, ten
Dialkyl group polyoxyethylene ether, dodecyl polyoxyethylene sulfonic acid sodium and phenethyl phenol poly-oxypropylene polyoxyethylene ether, polyvinyl alcohol,
The mixture of one or more of dodecyl phenol polyethenoxy ether, lauryl sodium sulfate, Negel.
Further, the solvent for dimethylbenzene, diesel oil, benzene, cyclohexanol, isopropanol, soybean oil, turpentine-based vegetable oil,
The mixing of one or more of methyl oleate, solvent naphtha, rapeseed oil, ethyl alcohol, N- pyrrolidones, ethyl acetate and water
Object.
Further, the antifreezing agent is the mixed of one or more of ethylene glycol, propylene glycol, glycerine and urea
Close object.
Further, the thickener for one kind in xanthans, aluminum magnesium silicate, hydroxymethyl cellulose and polyvinyl alcohol or
Two or more mixtures.
Further, the antifoaming agent is one or more of silicone oil, organic silicone and polypropylene glycerol aether
Mixture.
Further, the wetting agent is lauryl sodium sulfate, calcium dodecyl benzene sulfonate, methyl naphthalene sulfonic acid sodium formaldehyde
The mixture of one or more of condensation product, pull open powder, Negel and spaonin powder.
Further, the disintegrant for one kind in bentonite, ammonium sulfate, sodium bicarbonate, aluminium chloride and succinic acid or
Two or more mixtures.
Further, the filler is one kind or two in white carbon, kaolin, diatomite, precipitated calcium carbonate and starch
Kind or more mixture.
The second object of the present invention is to provide the preparation method of the above-mentioned missible oil containing copper rosinate and pyraclostrobin.This
The preparation method of invention is simple, wide market, is suitble to large-scale promotion application.
The technical solution that the present invention solves above-mentioned technical problem is as follows：A kind of missible oil containing copper rosinate and pyraclostrobin
Preparation method, include the following steps：
Copper rosinate and pyraclostrobin are taken, the parts by weight of the copper rosinate are 1-30 parts, the pyraclostrobin
Parts by weight for 1-40 parts, after mixing to get the missible oil containing copper rosinate and pyraclostrobin；
Or copper rosinate, pyraclostrobin and auxiliary agent are taken, the parts by weight of the copper rosinate are 1-30 parts, the pyrazoles
The parts by weight of kresoxim-methyl are 1-40 parts, and the ratio of the weight of the total weight and auxiliary agent of the copper rosinate and pyraclostrobin is
1：(0.8-1), after mixing to get the missible oil containing copper rosinate and pyraclostrobin.
The third object of the present invention is to provide above-mentioned the answering as the missible oil of active constituent using copper rosinate and pyraclostrobin
With.The missible oil of the present invention can be applied to citrus red mite, citrus rust mite, citrus resin disease, anthracnose of orange, citrus frost
Mildew, citrus ulcer, citrus gray mold and the prevention of citrus whitefly aphid.
The technical solution that the present invention solves above-mentioned technical problem is as follows：It is a kind of to contain copper rosinate and pyrazoles ether as described above
The missible oil of bacterium ester is burst in prevention citrus red mite, citrus rust mite, citrus resin disease, anthracnose of orange, citrus downy mildew, citrus
Application on ulcer disease, citrus gray mold and citrus whitefly aphid.
Beneficial effects of the present invention：
(1) present invention uses copper rosinate and pyraclostrobin that missible oil is made for raw material, can generate higher synergy and make
With expansion broad-spectrum overcomes and pathogen is delayed to develop immunity to drugs.Preventive effect is better than single dose, resistance of rainwater washing against, quick-acting are good,
Lasting period is long, reduces access times, time saving, laborsaving, can prevent a variety of pathogens and disease simultaneously, reduce drug cost.
Especially to citrus red mite, citrus rust mite, citrus resin disease, anthracnose of orange, citrus downy mildew, citrus ulcer, citrus
Gray mold and citrus whitefly aphid have apparent synergistic effect, and control effect is good.
(2) preparation method of the invention is simple, wide market, is suitble to large-scale production.
(3) missible oil of the invention, can be applied to citrus red mite, citrus rust mite, citrus resin disease, citrus anthrax
The prevention of disease, citrus downy mildew, citrus ulcer, citrus gray mold and citrus whitefly aphid.
Specific embodiment
The principle of the present invention and feature are described below in conjunction with specific embodiment, example is served only for explaining this hair
It is bright, it is not intended to limit the scope of the present invention.
Embodiment 1
The missible oil containing copper rosinate and pyraclostrobin of the present embodiment is the pyrazoles ether bacterium that mass percent is 40%
Ester copper rosinate missible oil, is made of the raw material of following weight：Copper rosinate 10g, pyraclostrobin 30g, styrylphenol
Polyoxyethylene ether 7g, polyoxyethylene carboxylate sodium phosphate 4g, lauryl sodium sulfate 2g, polyvinyl alcohol 3.1g, fatty amine gather
Ethylene oxide ether 4g, dimethylbenzene 8g and soybean oil 31.9g.
The preparation method of the above-mentioned missible oil containing copper rosinate and pyraclostrobin, includes the following steps：Take copper rosinate, pyrrole
Azoles kresoxim-methyl, styrylphenol polyoxyethylene ether, polyoxyethylene carboxylate sodium phosphate, lauryl sodium sulfate, polyethylene
Alcohol, aliphatic amine polyoxyethylene ether, dimethylbenzene and soybean oil after mixing, carry out high speed shearing emulsification to get containing copper rosinate
With the missible oil of pyraclostrobin.
The above-mentioned missible oil containing copper rosinate and pyraclostrobin is in prevention citrus red mite, citrus rust mite, citrus resin
Application on disease, anthracnose of orange, citrus downy mildew, citrus ulcer, citrus gray mold and citrus whitefly aphid.
Embodiment 2
The missible oil containing copper rosinate and pyraclostrobin of the present embodiment, is made of the raw material of following weight：Copper rosinate
1g, pyraclostrobin 1g, phenethyl phenol poly-oxypropylene polyoxyethylene ether 2g, alkyl phenol polyoxyethylene ether phosphoesterase 30 .5g, alkyl
Phenol polyethenoxy ether 2g, sodium polycarboxylate 2g, cyclohexanol 9g, isopropanol 5g and methyl oleate 78.5g.
The preparation method of the above-mentioned missible oil containing copper rosinate and pyraclostrobin, includes the following steps：Take copper rosinate, pyrrole
Azoles kresoxim-methyl, phenethyl phenol poly-oxypropylene polyoxyethylene ether, alkyl phenol polyoxyethylene ether phosphate, alkyl phenol polyoxyethylene ether,
Sodium polycarboxylate, cyclohexanol, isopropanol and methyl oleate, after mixing, carry out high speed shearing emulsification to get containing copper rosinate and
The missible oil of pyraclostrobin.
The above-mentioned missible oil containing copper rosinate and pyraclostrobin is in prevention citrus red mite, citrus rust mite, citrus resin
Application on disease, anthracnose of orange, citrus downy mildew, citrus ulcer, citrus gray mold and citrus whitefly aphid.
Embodiment 3
The missible oil containing copper rosinate and pyraclostrobin of the present embodiment is the pyrazoles ether bacterium that mass percent is 50%
Ester copper rosinate missible oil, is made of the raw material of following weight：Copper rosinate 10g, pyraclostrobin 40g, sodium lignin sulfonate
4g, polyoxyethylene carboxylate sodium phosphate 2g, dodecyl phenol polyethenoxy ether 1g, styrylphenol polyoxyethylene ether 3g,
Diesel oil 5g, dimethylbenzene 2g and solvent naphtha 30g.
The preparation method of the above-mentioned missible oil containing copper rosinate and pyraclostrobin, includes the following steps：Take copper rosinate, pyrrole
Azoles kresoxim-methyl, sodium lignin sulfonate, polyoxyethylene carboxylate sodium phosphate, dodecyl phenol polyethenoxy ether, styryl benzene
Phenol polyethenoxy ether, diesel oil, dimethylbenzene and solvent naphtha, after mixing, carry out high speed shearing emulsification to get containing copper rosinate and
The missible oil of pyraclostrobin.
The above-mentioned missible oil containing copper rosinate and pyraclostrobin is in prevention citrus red mite, citrus rust mite, citrus resin
Application on disease, anthracnose of orange, citrus downy mildew, citrus ulcer, citrus gray mold and citrus whitefly aphid.
Embodiment 4
The missible oil containing copper rosinate and pyraclostrobin of the present embodiment, is made of the raw material of following weight：Copper rosinate
30g, pyraclostrobin 10g, APES formaldehyde condensation product sodium sulphate 2g, Negel 2g, naphthalene sulfonic acids first
Aldehyde condensate sodium salt 2g, aliphatic amine polyoxyethylene ether 2g, phenethyl phenol poly-oxypropylene polyoxyethylene ether 2g, glycerin polyoxyethylene ether
Aliphatic ester 4g, N- pyrrolidones 0.5g, ethyl acetate 4g and soybean oil 39.5g.
The preparation method of the above-mentioned missible oil containing copper rosinate and pyraclostrobin, includes the following steps：Take copper rosinate, pyrrole
Azoles kresoxim-methyl, APES formaldehyde condensation product sodium sulphate, Negel, naphthalene sulfonic acid-formaldehyde condensation product sodium salt,
Aliphatic amine polyoxyethylene ether, phenethyl phenol poly-oxypropylene polyoxyethylene ether, glycerin polyoxyethylene ether fatty ester, N- pyrrolidines
Ketone, ethyl acetate and soybean oil after mixing, carry out high speed shearing emulsification to get containing copper rosinate and pyraclostrobin
Missible oil.
The above-mentioned missible oil containing copper rosinate and pyraclostrobin is in prevention citrus red mite, citrus rust mite, citrus resin
Application on disease, anthracnose of orange, citrus downy mildew, citrus ulcer, citrus gray mold and citrus whitefly aphid.
Embodiment 5
The missible oil containing copper rosinate and pyraclostrobin of the present embodiment is the pyrazoles ether bacterium that mass percent is 30%
Ester copper rosinate missible oil, is made of the raw material of following weight：Copper rosinate 10g, pyraclostrobin 20g, glycerin polyoxyethylene
Ether fatty acid ester 5g, calcium dodecyl benzene sulfonate 2g, aliphatic acid polyethenoxy base ester 2g, ethyl alcohol 0.1g, methyl oleate 12g, dish
Seed oil 3g and turpentine-based vegetable oil 43g.
The preparation method of the above-mentioned missible oil containing copper rosinate and pyraclostrobin, includes the following steps：Take copper rosinate, pyrrole
Azoles kresoxim-methyl, glycerin polyoxyethylene ether fatty ester, calcium dodecyl benzene sulfonate, aliphatic acid polyethenoxy base ester, ethyl alcohol, oleic acid
Methyl esters, rapeseed oil and turpentine-based vegetable oil after mixing, carry out high speed shearing emulsification to get containing copper rosinate and pyrazoles ether
The missible oil of bacterium ester.
The above-mentioned missible oil containing copper rosinate and pyraclostrobin is in prevention citrus red mite, citrus rust mite, citrus resin
Application on disease, anthracnose of orange, citrus downy mildew, citrus ulcer, citrus gray mold and citrus whitefly aphid.
Application Example：
In order to detect the control effect of the composition of the present invention, tests below data are now enumerated：
Fountain height after following table 1 to the medicament dilution of table 3 is by the positive back of the body of the conventional spray requirement of this field, i.e. leaf
Face, fruit surface, which are sprayed to, to be formed drop-wise and flows down.
Application Example 1
The 40% pyraclostrobin copper rosinate missible oil and 25% pyraclostrobin that the embodiment of the present invention 1 is obtained are wettable
Property pulvis, 30% copper rosinate missible oil carry out field control effectiveness test, test site is mono- teams of land reclamation and cultivation Guilin, Guilin City Guangxi Liang Feng
Navel orange orchard, spray-on process using double dilution method during preparating liquid, are uniformly sprayed on citrus branches and leaves and fruit, are sent out in resin disease
Sick initial stage carries out first time dispenser, applies 4 times, is investigated altogether within 20 days after last medicine, and investigation area is calculated using cell (per cell as 2-
3 plants of mandarin trees), investigate 2 plants of trees per cell, every plant by east, south, west, north, in the sampling of five points, two branches of every investigation, often
A branch investigates top 4 leaves and whole fruits down, records total leaf (fruit) number, sick leaf (fruit) number and sick grade of investigation, calculates
Disease index and preventive effect.
Result of the test is as shown in table 1.
Pyraclostrobin copper rosinate emulsion prevention citrus resin disease (rubber disease) drug effect examination that 1 embodiment 1 of table obtains
Test result
1 result of the test of table shows to start to apply first time medicine in citrus resin disease (rubber disease) early period of origination, is thanking to colored back seat
Fruit middle and later periods, young fruit period, fruit growth period are respectively applied once again, and the pyraclostrobin copper rosinate missible oil that embodiment 1 obtains is prevented
It controls citrus resin disease (rubber disease) to spray by 1500 times of dilutions, there is preferable preventive effect.
Application Example 3
The 50% pyraclostrobin copper rosinate missible oil and 25% pyraclostrobin that the embodiment of the present invention 3 is obtained are wettable
Property pulvis, 30% copper rosinate missible oil carry out field control effectiveness test, test site is mono- teams of land reclamation and cultivation Guilin, Guilin City Guangxi Liang Feng
Navel orange orchard, foliar spray method are watered dilution by processing dosage and are made into liquid, be uniformly sprayed on citrus leaf tow sides,
First time dispenser is carried out in anthracnose early stage, applies 4 times, is investigated within 20 days after last medicine, investigation area is calculated with cell altogether
(being 2-3 plant mandarin trees per cell), investigates 2 plants of trees per cell, every plant by east, south, west, north, in the sampling of five points, every is investigated
Two branches, 4 leaves and whole fruits, total leaf (fruit) number, the disease leaf (fruit) for recording investigation count each branch investigation top down
With sick grade, disease index and preventive effect are calculated.
Result of the test is as shown in table 2.
The pyraclostrobin copper rosinate emulsion prevention citrus anthracnose test of pesticide effectiveness result that 2 embodiment 3 of table obtains
2 result of the test of table shows in mandarin tree anthracnose early period of origination dispenser, the pyraclostrobin that embodiment 3 obtains
Copper rosinate missible oil sprays by amount used above, has preferable preventive effect to prevention citrus anthracnose.
Application Example 5
The 30% pyraclostrobin copper rosinate missible oil and 25% pyraclostrobin that the embodiment of the present invention 5 is obtained are wettable
Property pulvis, 30% copper rosinate missible oil carry out field control effectiveness test, test site is mono- teams of land reclamation and cultivation Guilin, Guilin City Guangxi Liang Feng
Navel orange orchard, foliar spray method are watered dissolved dilution by processing dosage and are made into liquid, first is carried out in gray mold early stage
Secondary dispenser is applied 4 times altogether, is investigated within 20 days after last medicine, and investigation area calculates (per cell as 2-3 plants of mandarin trees) using cell, often
Cell investigates 2 plants of trees, every plant by east, south, west, north, in the sampling of five points, two branches of every investigation, each branch investigation top
Leaf 4 leaves and whole fruit down record total leaf (fruit) number, sick leaf (fruit) number and sick grade of investigation, calculate disease index and anti-
Effect.
Result of the test is as shown in table 3.
The pyraclostrobin copper rosinate emulsion prevention citrus gray mold test of pesticide effectiveness result that 3 embodiment 5 of table obtains
3 result of the test of table shows to endanger phase dispenser, the pyraclostrobin rosin that embodiment 5 obtains in citrus gray mold
Sour copper missible oil has preferable preventive effect in the case of amount used above, to prevention citrus gray mold.
Following instance illustrates the synergistic effect of the missible oil containing copper rosinate and pyraclostrobin
1st, the missible oil containing copper rosinate and pyraclostrobin of the invention is to the Toxicity Determination of citrus blister mite
Subjects are citrus blister mite.Experiment is using medicine embrane method.Take cylindrical transparent glass preserving jar (base diameter
6.8cm, bottleneck diameter 5.5cm, high 8.7cm), the liquid that 50ml has been diluted to required concentration is added in, lid is covered tightly, gently shakes
It is even, 30s is inverted, a bottle inner wall is made all to stick liquid, then pours out liquid, then allows bottle naturally dry.Citrus tender shoots is used
Identical liquid impregnates 1min, is put into the bottle dried after naturally dry；Tender shoots base portion is encased with the cotton of dipped identical liquid
Moisturizing.Blister mite adult is acquired with band plug teat glass from kamuning tree.Each processing is repeated 3 times, repeat every time 10 into
Worm.CO is used during processing2It is put into after anesthesia (18-20s) in each processing bottle, blank control clear water, 40 mesh yarns of each bottleneck
Square is good, flies out to prevent adult.Dead worm is checked whether there is after twenty minutes, records borer population living before medicine.The vial after worm will be connect to put
In the illumination box of temperature (25 ± 1) DEG C.Dead borer population, borer population living are estimated after medicine for 24 hours, calculates the death rate, clear water control is dead
It is efficiency test to die rate no more than 10%.First by concentration conversion into natural logrithm value, corrected mortality is converted into arcsine transformation
Value.Again using arcsine transformation value as dependent variable, using dosage natural logrithm value as independent variable, carried out directly with DPS Biological Statistic Analysis Softwares
Line regression analysis obtains the toxicity regression linear equation of each medicament.The toxicity index of medicament is calculated according to the abundant methods of Sun Yun and is total to
Malicious coefficient (CTC).
Survey toxicity index=standard agent EC50/ reagent agent EC50×100
Single dose theoretical toxicity index=content of the single dose actual measurement toxicity index × single dose in mixture
The sum of mixture theoretical toxicity index=each single dose theoretical toxicity index
Toxicity index/mixture theoretical toxicity index × 100 of co-toxicity coefficient=mixture actual measurement
Evaluation criterion：CTC≤80 are antagonism, and 80 ＜ CTC ＜ 120 are summation action, and CTC >=120 are synergistic effect.
Result of the test is as shown in table 4.
The toxicity index of 4 each medicament of table and the co-toxicity coefficient of pyraclostrobin copper rosinate
| Reagent agent | Quality proportioning | EC50(mg/kg) | Co-toxicity coefficient |
| Pyraclostrobin | -- | 6.42 | - |
| Copper rosinate | -- | 4.61 | - |
| Pyraclostrobin：Copper rosinate | 40:1 | 4.01 | 158.58 |
| Pyraclostrobin：Copper rosinate | 30:1 | 3.72 | 170.42 |
| Pyraclostrobin：Copper rosinate | 20:1 | 3.46 | 182.14 |
| Pyraclostrobin：Copper rosinate | 15:1 | 3.22 | 194.60 |
| Pyraclostrobin：Copper rosinate | 10:1 | 2.96 | 209.42 |
| Pyraclostrobin：Copper rosinate | 1:1 | 2.46 | 218.15 |
| Pyraclostrobin：Copper rosinate | 1:5 | 2.54 | 190.44 |
| Pyraclostrobin：Copper rosinate | 1:10 | 2.68 | 176.54 |
| Pyraclostrobin：Copper rosinate | 1:15 | 2.86 | 164.08 |
| Pyraclostrobin：Copper rosinate | 1:20 | 3.01 | 155.24 |
| Pyraclostrobin：Copper rosinate | 1:30 | 3.33 | 139.71 |
2nd, the missible oil containing copper rosinate and pyraclostrobin of the invention is to the Toxicity Determination of citrus red mite：
The glass slide infusion process that experiment is recommended using FAO (Food and Agriculture Organization of the United Nation) (FAO).Every piece of glass slide be inoculated with 20 it is living
It is female to set series concentration value on the basis of 3 pilot studies into mite, clear water control, often handle 3 repetitions (totally 60 it is female into
Mite).Leaching medicine is blotted after 5 seconds with blotting paper immediately, is then placed in 24-26 DEG C of insulating box, is checked within 48 hours after medicine dead
Rate calculates corrected mortality.By concentration conversion into natural logrithm value, corrected mortality is converted into arcsine transformation value, then with anti-
Sinusoidal replacement value is dependent variable, and using dosage natural logrithm value as independent variable, linear regression point is carried out with DPS Biological Statistic Analysis Softwares
Analysis obtains the toxicity regression linear equation of each medicament.It is dead that correction is obtained according to the toxicity regression linear equation of each medicament
Rate arcsine transformation value (y) obtains EC by looking into antilogarithm table50Value, then according to the abundant methods of Sun Yun calculate medicament toxicity index and
Co-toxicity coefficient (CTC).
Survey toxicity index=standard agent EC50/ reagent agent EC50×100
Single dose theoretical toxicity index=content of the single dose actual measurement toxicity index × single dose in mixture
The sum of mixture theoretical toxicity index=each single dose theoretical toxicity index
Toxicity index/mixture theoretical toxicity index × 100 of co-toxicity coefficient=mixture actual measurement
Evaluation criterion：CTC≤80 are antagonism, and 80 ＜ CTC ＜ 120 are summation action, and CTC >=120 are synergistic effect.
Result of the test is as shown in table 5.
The toxicity index of 5 each medicament of table and the co-toxicity coefficient of pyraclostrobin copper rosinate
| Reagent agent | Quality proportioning | EC50(mg/kg) | Co-toxicity coefficient |
| Copper rosinate | -- | 7.46 | - |
| Pyraclostrobin | -- | 6.01 | - |
| Copper rosinate：Pyraclostrobin | 1:40 | 4.82 | 153.87 |
| Copper rosinate：Pyraclostrobin | 1:30 | 4.61 | 160.57 |
| Copper rosinate：Pyraclostrobin | 1:20 | 4.31 | 171.12 |
| Copper rosinate：Pyraclostrobin | 1:15 | 4.01 | 183.27 |
| Copper rosinate：Pyraclostrobin | 1:10 | 3.79 | 192.61 |
| Copper rosinate：Pyraclostrobin | 1:1 | 3.32 | 200.51 |
| Copper rosinate：Pyraclostrobin | 5:1 | 3.01 | 206.35 |
| Copper rosinate：Pyraclostrobin | 10:1 | 3.12 | 196.09 |
| Copper rosinate：Pyraclostrobin | 15:1 | 3.55 | 171.38 |
| Copper rosinate：Pyraclostrobin | 20:1 | 3.72 | 163.07 |
| Copper rosinate：Pyraclostrobin | 30:1 | 3.89 | 155.47 |
3rd, the missible oil containing copper rosinate and pyraclostrobin of the invention is to the Toxicity Determination of mandarin tree canker：
Experiment takes the experiment liquid that 5ml is prepared to be added to equipped with the hot culture medium (PDA of 45ml using mycelial growth rate method
Culture medium, 45-50 DEG C) conical flask in, after shaking up, pour into diameter 90mm glass culture dish rapidly, each culture dish pours into band
Medicine culture medium 12ml, each handle 4 repetitions, horizontal rest, after cooling tablet.With diameter 5mm card punch from culture 4d
Cut bacteria cake for examination bacterium edge, with needle is chosen by being connected on one side on the malicious culture medium of band with mycelia, all operations are ultra-clean
Workbench carries out sterile working.Tablet is placed in 20 DEG C of constant temperature sterile culture case after processing and uses right-angled intersection after culture 4d
Method measures the colony diameter of each processing respectively, calculates the average value of each processing colony diameter, the average net growth of colony diameter
And mycelial growth inhibition rate.
Bacterium colony net growth (mm)=colony diameter -5
Mycelial growth inhibition rate (%)=((control bacterium colony net growth-processing bacterium colony net growth)/control bacterium colony is net
Increment) × 100
By concentration conversion into natural logrithm value, inhibit rate conversion arcsin function value, using arcsine letter replacement value as because becoming
Amount, using dosage natural logrithm value as independent variable, carries out linear regression analysis with DPS Biological Statistic Analysis Softwares, obtains regression equation, then
It carries out anti-natural logrithm conversion and obtains EC50.The toxicity index of medicament and co-toxicity coefficient (CTC) are calculated according to the abundant methods of Sun Yun.
Survey toxicity index=standard agent EC50/ reagent agent EC50×100
Single dose theoretical toxicity index=content of the single dose actual measurement toxicity index × single dose in mixture
The sum of mixture theoretical toxicity index=each single dose theoretical toxicity index
Toxicity index/mixture theoretical toxicity index × 100 of co-toxicity coefficient=mixture actual measurement
Evaluation criterion：CTC≤80 are antagonism, and 80 ＜ CTC ＜ 120 are summation action, and CTC >=120 are synergistic effect.
Result of the test is as shown in table 6.
The toxicity index of 6 each medicament of table and the co-toxicity coefficient of pyraclostrobin copper rosinate
| Reagent agent | Quality proportioning | EC50(mg/kg) | Co-toxicity coefficient |
| Copper rosinate | - | 9.02 | - |
| Pyraclostrobin | - | 7.68 | - |
| Copper rosinate:Pyraclostrobin | 1:40 | 5.01 | 153.85 |
| Copper rosinate:Pyraclostrobin | 1:30 | 4.75 | 162.46 |
| Copper rosinate:Pyraclostrobin | 1:20 | 4.48 | 172.65 |
| Copper rosinate:Pyraclostrobin | 1:15 | 4.21 | 184.13 |
| Copper rosinate:Pyraclostrobin | 1:10 | 4.03 | 193.18 |
| Copper rosinate:Pyraclostrobin | 1:1 | 3.88 | 213.82 |
| Copper rosinate:Pyraclostrobin | 5:1 | 3.92 | 223.60 |
| Copper rosinate:Pyraclostrobin | 10:1 | 4.81 | 184.60 |
| Copper rosinate:Pyraclostrobin | 15:1 | 5.02 | 177.74 |
| Copper rosinate:Pyraclostrobin | 20:1 | 5.68 | 157.49 |
| Copper rosinate:Pyraclostrobin | 30:1 | 5.98 | 149.99 |
The result of the test of table 6 shows copper rosinate and pyraclostrobin composition in mass ratio 1:40~30:1 compounding is right
Mandarin tree canker has synergistic effect, especially matches 1：40~20：When between 1, synergy is more notable.
4th, the missible oil containing copper rosinate and pyraclostrobin of the invention is to the Toxicity Determination of citrus anthracnose：
Experiment takes the experiment liquid that 5ml is prepared to be added to equipped with the hot culture medium (PDA of 45m l using mycelial growth rate method
Culture medium, 45-50 DEG C) conical flask in, after shaking up, pour into diameter 90mm glass culture dish rapidly, each culture dish pours into band
Medicine culture medium 12ml, each handle 4 repetitions, horizontal rest, after cooling tablet.With diameter 5mm card punch from culture 4d
Cut bacteria cake for examination bacterium edge, with needle is chosen by being connected on one side on the malicious culture medium of band with mycelia, all operations are ultra-clean
Workbench carries out sterile working.Tablet is placed in 20 DEG C of constant temperature sterile culture case after processing and uses right-angled intersection after culture 4d
Method measures the colony diameter of each processing respectively, calculates the average value of each processing colony diameter, the average net growth of colony diameter
And mycelial growth inhibition rate.
Bacterium colony net growth (mm)=colony diameter -5
Mycelial growth inhibition rate (%)=((control bacterium colony net growth-processing bacterium colony net growth)/control bacterium colony is net
Increment) × 100
By concentration conversion into natural logrithm value, inhibit rate conversion arcsin function value, using arcsine letter replacement value as because becoming
Amount, using dosage natural logrithm value as independent variable, carries out linear regression analysis with DPS Biological Statistic Analysis Softwares, obtains regression equation, then
It carries out anti-natural logrithm conversion and obtains EC50.The toxicity index of medicament and co-toxicity coefficient (CTC) are calculated according to the abundant methods of Sun Yun.
Survey toxicity index=standard agent EC50/ reagent agent EC50×100
Single dose theoretical toxicity index=content of the single dose actual measurement toxicity index × single dose in mixture
The sum of mixture theoretical toxicity index=each single dose theoretical toxicity index
Toxicity index/mixture theoretical toxicity index × 100 of co-toxicity coefficient=mixture actual measurement
Evaluation criterion：CTC≤80 are antagonism, and 80 ＜ CTC ＜ 120 are summation action, and CTC >=120 are synergistic effect.
Result of the test is as shown in table 7.
The toxicity index of 7 each medicament of table and the co-toxicity coefficient of pyraclostrobin copper rosinate
| Reagent agent | Quality proportioning | EC50(mg/kg) | Co-toxicity coefficient |
| Copper rosinate | - | 8.46 | - |
| Pyraclostrobin | - | 6.02 | - |
| Copper rosinate:Pyraclostrobin | 1:40 | 3.99 | 151.95 |
| Copper rosinate:Pyraclostrobin | 1:30 | 3.45 | 176.13 |
| Copper rosinate:Pyraclostrobin | 1:20 | 3.31 | 184.41 |
| Copper rosinate:Pyraclostrobin | 1:15 | 3.01 | 203.67 |
| Copper rosinate:Pyraclostrobin | 1:10 | 2.88 | 214.66 |
| Copper rosinate:Pyraclostrobin | 1:1 | 3.34 | 210.61 |
| Copper rosinate:Pyraclostrobin | 5:1 | 3.99 | 198.61 |
| Copper rosinate:Pyraclostrobin | 10:1 | 4.32 | 188.87 |
| Copper rosinate:Pyraclostrobin | 15:1 | 4.76 | 173.34 |
| Copper rosinate:Pyraclostrobin | 20:1 | 5.01 | 165.66 |
| Copper rosinate:Pyraclostrobin | 30:1 | 5.51 | 151.56 |
The result of the test of table 7 shows copper rosinate and pyraclostrobin composition in mass ratio 1:40~30:1 compounding is right
Citrus anthracnose has synergistic effect, especially matches 1:30~20:When 1, synergy is more notable.
5th, the missible oil containing copper rosinate and pyraclostrobin of the invention is to the Toxicity Determination of citrus black rot：
Experiment takes the experiment liquid that 5ml is prepared to be added to equipped with the hot culture medium (PDA of 45m l using mycelial growth rate method
Culture medium, 45-50 DEG C) conical flask in, after shaking up, pour into diameter 90mm glass culture dish rapidly, each culture dish pours into band
Medicine culture medium 12ml, each handle 4 repetitions, horizontal rest, after cooling tablet.With diameter 5mm card punch from culture 4d
Cut bacteria cake for examination bacterium edge, with needle is chosen by being connected on one side on the malicious culture medium of band with mycelia, all operations are ultra-clean
Workbench carries out sterile working.Tablet is placed in 20 DEG C of constant temperature sterile culture case after processing and uses right-angled intersection after culture 4d
Method measures the colony diameter of each processing respectively, calculates the average value of each processing colony diameter, the average net growth of colony diameter
And mycelial growth inhibition rate.
Bacterium colony net growth (mm)=colony diameter -5
Mycelial growth inhibition rate (%)=((control bacterium colony net growth-processing bacterium colony net growth)/control bacterium colony is net
Increment) × 100
By concentration conversion into natural logrithm value, inhibit rate conversion arcsin function value, using arcsine letter replacement value as because becoming
Amount, using dosage natural logrithm value as independent variable, carries out linear regression analysis with DPS Biological Statistic Analysis Softwares, obtains regression equation, then
It carries out anti-natural logrithm conversion and obtains EC50.The toxicity index of medicament and co-toxicity coefficient (CTC) are calculated according to the abundant methods of Sun Yun.
Survey toxicity index=standard agent EC50/ reagent agent EC50×100
Single dose theoretical toxicity index=content of the single dose actual measurement toxicity index × single dose in mixture
The sum of mixture theoretical toxicity index=each single dose theoretical toxicity index
Toxicity index/mixture theoretical toxicity index × 100 of co-toxicity coefficient=mixture actual measurement
Evaluation criterion：CTC≤80 are antagonism, and 80 ＜ CTC ＜ 120 are summation action, and CTC >=120 are synergistic effect.
Result of the test is as shown in table 8.
The toxicity index of 8 each medicament of table and the co-toxicity coefficient of pyraclostrobin copper rosinate
| Reagent agent | Quality proportioning | EC50(mg/kg) | Co-toxicity coefficient |
| Copper rosinate | - | 7.66 | - |
| Pyraclostrobin | - | 5.63 | - |
| Copper rosinate:Pyraclostrobin | 1:40 | 3.76 | 150.71 |
| Copper rosinate:Pyraclostrobin | 1:30 | 3.42 | 166.04 |
| Copper rosinate:Pyraclostrobin | 1:20 | 3.01 | 189.43 |
| Copper rosinate:Pyraclostrobin | 1:15 | 2.91 | 196.73 |
| Copper rosinate:Pyraclostrobin | 1:10 | 2.78 | 207.52 |
| Copper rosinate:Pyraclostrobin | 1:1 | 2.98 | 217.78 |
| Copper rosinate:Pyraclostrobin | 5:1 | 3.36 | 215.05 |
| Copper rosinate:Pyraclostrobin | 10:1 | 3.91 | 189.69 |
| Copper rosinate:Pyraclostrobin | 15:1 | 4.21 | 177.94 |
| Copper rosinate:Pyraclostrobin | 20:1 | 4.68 | 160.91 |
| Copper rosinate:Pyraclostrobin | 30:1 | 4.98 | 152.05 |
The result of the test of table 8 shows copper rosinate and pyraclostrobin composition in mass ratio 1:40~30:1 compounding is right
Citrus black rot has synergistic effect, especially matches 1:30~20:When 1, synergy is more notable.
6th, the missible oil containing copper rosinate and pyraclostrobin of the invention is to the Toxicity Determination of citrus downy mildew：
Experiment takes the experiment liquid that 5ml is prepared to be added to equipped with the hot culture medium (PDA of 45m l using mycelial growth rate method
Culture medium, 45-50 DEG C) conical flask in, after shaking up, pour into diameter 90mm glass culture dish rapidly, each culture dish pours into band
Medicine culture medium 12ml, each handle 4 repetitions, horizontal rest, after cooling tablet.With diameter 5mm card punch from culture 4d
Cut bacteria cake for examination bacterium edge, with needle is chosen by being connected on one side on the malicious culture medium of band with mycelia, all operations are ultra-clean
Workbench carries out sterile working.Tablet is placed in 20 DEG C of constant temperature sterile culture case after processing and uses right-angled intersection after culture 4d
Method measures the colony diameter of each processing respectively, calculates the average value of each processing colony diameter, the average net growth of colony diameter
And mycelial growth inhibition rate.
Bacterium colony net growth (mm)=colony diameter -5
Mycelial growth inhibition rate (%)=((control bacterium colony net growth-processing bacterium colony net growth)/control bacterium colony is net
Increment) × 100
By concentration conversion into natural logrithm value, inhibit rate conversion arcsin function value, using arcsine letter replacement value as because becoming
Amount, using dosage natural logrithm value as independent variable, carries out linear regression analysis with DPS Biological Statistic Analysis Softwares, obtains regression equation, then
It carries out anti-natural logrithm conversion and obtains EC50.The toxicity index of medicament and co-toxicity coefficient (CTC) are calculated according to the abundant methods of Sun Yun.
Survey toxicity index=standard agent EC50/ reagent agent EC50×100
Single dose theoretical toxicity index=content of the single dose actual measurement toxicity index × single dose in mixture
The sum of mixture theoretical toxicity index=each single dose theoretical toxicity index
Toxicity index/mixture theoretical toxicity index × 100 of co-toxicity coefficient=mixture actual measurement
Evaluation criterion：CTC≤80 are antagonism, and 80 ＜ CTC ＜ 120 are summation action, and CTC >=120 are synergistic effect.
Result of the test is as shown in table 9.
The toxicity index of 9 each medicament of table and the co-toxicity coefficient of pyraclostrobin copper rosinate
| Reagent agent | Quality proportioning | EC50(mg/kg) | Co-toxicity coefficient |
| Copper rosinate | -- | 7.62 | - |
| Pyraclostrobin | -- | 6.02 | - |
| Copper rosinate:Pyraclostrobin | 1:40 | 3.99 | 151.65 |
| Copper rosinate:Pyraclostrobin | 1:30 | 3.61 | 167.90 |
| Copper rosinate:Pyraclostrobin | 1:20 | 3.41 | 178.32 |
| Copper rosinate:Pyraclostrobin | 1:15 | 3.12 | 195.51 |
| Copper rosinate:Pyraclostrobin | 1:10 | 3.01 | 203.89 |
| Copper rosinate:Pyraclostrobin | 1:1 | 2.98 | 225.71 |
| Copper rosinate:Pyraclostrobin | 5:1 | 3.98 | 183.34 |
| Copper rosinate:Pyraclostrobin | 10:1 | 4.11 | 181.03 |
| Copper rosinate:Pyraclostrobin | 15:1 | 4.46 | 168.06 |
| Copper rosinate:Pyraclostrobin | 20:1 | 4.67 | 161.13 |
| Copper rosinate:Pyraclostrobin | 30:1 | 4.99 | 151.41 |
The result of the test of table 9 shows copper rosinate and pyraclostrobin composition in mass ratio 1:40~30:1 compounding is right
Citrus downy mildew has synergistic effect, especially matches 1:30~20:When 1, synergy is more notable.
7th, the missible oil containing copper rosinate and pyraclostrobin of the invention is to the Toxicity Determination of citrus gray mold：
Experiment takes the experiment liquid that 5ml is prepared to be added to equipped with the hot culture medium (PDA of 45m l using mycelial growth rate method
Culture medium, 45-50 DEG C) conical flask in, after shaking up, pour into diameter 90mm glass culture dish rapidly, each culture dish pours into band
Medicine culture medium 12ml, each handle 4 repetitions, horizontal rest, after cooling tablet.With diameter 5mm card punch from culture 4d
Cut bacteria cake for examination bacterium edge, with needle is chosen by being connected on one side on the malicious culture medium of band with mycelia, all operations are ultra-clean
Workbench carries out sterile working.Tablet is placed in 20 DEG C of constant temperature sterile culture case after processing and uses right-angled intersection after culture 4d
Method measures the colony diameter of each processing respectively, calculates the average value of each processing colony diameter, the average net growth of colony diameter
And mycelial growth inhibition rate.
Bacterium colony net growth (mm)=colony diameter -5
Mycelial growth inhibition rate (%)=((control bacterium colony net growth-processing bacterium colony net growth)/control bacterium colony is net
Increment) × 100
By concentration conversion into natural logrithm value, inhibit rate conversion arcsin function value, using arcsine letter replacement value as because becoming
Amount, using dosage natural logrithm value as independent variable, carries out linear regression analysis with DPS Biological Statistic Analysis Softwares, obtains regression equation, then
It carries out anti-natural logrithm conversion and obtains EC50.The toxicity index of medicament and co-toxicity coefficient (CTC) are calculated according to the abundant methods of Sun Yun.
Survey toxicity index=standard agent EC50/ reagent agent EC50×100
Single dose theoretical toxicity index=content of the single dose actual measurement toxicity index × single dose in mixture
The sum of mixture theoretical toxicity index=each single dose theoretical toxicity index
Toxicity index/mixture theoretical toxicity index × 100 of co-toxicity coefficient=mixture actual measurement
Evaluation criterion：CTC≤80 are antagonism, and 80 ＜ CTC ＜ 120 are summation action, and CTC >=120 are synergistic effect.
Result of the test is as shown in table 10.
The toxicity index of 10 each medicament of table and the co-toxicity coefficient of pyraclostrobin copper rosinate
| Reagent agent | Quality proportioning | EC50(mg/kg) | Co-toxicity coefficient |
| Copper rosinate | -- | 8.82 | - |
| Pyraclostrobin | -- | 6.02 | - |
| Copper rosinate:Pyraclostrobin | 1:40 | 3.99 | 152.05 |
| Copper rosinate:Pyraclostrobin | 1:30 | 3.63 | 167.56 |
| Copper rosinate:Pyraclostrobin | 1:20 | 3.39 | 180.31 |
| Copper rosinate:Pyraclostrobin | 1:15 | 3.11 | 197.49 |
| Copper rosinate:Pyraclostrobin | 1:10 | 2.99 | 207.32 |
| Copper rosinate:Pyraclostrobin | 1:1 | 3.21 | 222.92 |
| Copper rosinate:Pyraclostrobin | 5:1 | 3.88 | 210.97 |
| Copper rosinate:Pyraclostrobin | 10:1 | 4.13 | 204.90 |
| Copper rosinate:Pyraclostrobin | 15:1 | 4.78 | 179.31 |
| Copper rosinate:Pyraclostrobin | 20:1 | 4.98 | 173.27 |
| Copper rosinate:Pyraclostrobin | 30:1 | 5.32 | 163.34 |
The result of the test of table 10 shows copper rosinate and pyraclostrobin composition in mass ratio 1:40~30:1 compounding is right
Citrus gray mold has synergistic effect, especially matches 1:30~30:When 1, synergy is more notable.
8th, the missible oil containing copper rosinate and pyraclostrobin of the invention is to the Toxicity Determination of citrus blister mite：
The glass slide infusion process that experiment is recommended using FAO (Food and Agriculture Organization of the United Nation) (FAO).Every piece of glass slide be inoculated with 20 it is living
It is female to set series concentration value on the basis of 3 pilot studies into mite, clear water control, often handle 3 repetitions (totally 60 it is female into
Mite).Leaching medicine is blotted after 5 seconds with blotting paper immediately, is then placed in 24-26 DEG C of insulating box, is checked within 48 hours after medicine dead
Rate calculates corrected mortality.By concentration conversion into natural logrithm value, corrected mortality is converted into arcsine transformation value, then with anti-
Sinusoidal replacement value is dependent variable, and using dosage natural logrithm value as independent variable, linear regression point is carried out with DPS Biological Statistic Analysis Softwares
Analysis obtains the toxicity regression linear equation of each medicament.It is dead that correction is obtained according to the toxicity regression linear equation of each medicament
Rate arcsine transformation value (y) obtains EC by looking into antilogarithm table50Value, then according to the abundant methods of Sun Yun calculate medicament toxicity index and
Co-toxicity coefficient (CTC).
Survey toxicity index=standard agent EC50/ reagent agent EC50×100
Single dose theoretical toxicity index=content of the single dose actual measurement toxicity index × single dose in mixture
The sum of mixture theoretical toxicity index=each single dose theoretical toxicity index
Toxicity index/mixture theoretical toxicity index × 100 of co-toxicity coefficient=mixture actual measurement
Evaluation criterion：CTC≤80 are antagonism, and 80 ＜ CTC ＜ 120 are summation action, and CTC >=120 are synergistic effect.
Result of the test is as shown in table 11.
The toxicity index of 11 each medicament of table and the co-toxicity coefficient of pyraclostrobin copper rosinate
| Reagent agent | Quality proportioning | EC50(mg/kg) | Co-toxicity coefficient |
| Copper rosinate | - | 8.62 | - |
| Pyraclostrobin | - | 6.26 | - |
| Copper rosinate：Pyraclostrobin | 1:40 | 4.38 | 143.88 |
| Copper rosinate：Pyraclostrobin | 1:30 | 3.79 | 166.64 |
| Copper rosinate：Pyraclostrobin | 1:15 | 3.64 | 174.25 |
| Copper rosinate：Pyraclostrobin | 1:1 | 3.32 | 191.84 |
| Copper rosinate：Pyraclostrobin | 5:1 | 3.12 | 205.76 |
| Copper rosinate：Pyraclostrobin | 10:1 | 3.21 | 225.95 |
| Copper rosinate：Pyraclostrobin | 15:1 | 3.81 | 212.87 |
| Copper rosinate：Pyraclostrobin | 20:1 | 4.39 | 189.85 |
| Copper rosinate：Pyraclostrobin | 30:1 | 4.88 | 172.57 |
The result of the test of table 11 shows copper rosinate and pyraclostrobin in mass ratio 1:40~30:1 compounding, becomes rusty to citrus
Tick has synergistic effect, especially matches 1:30~30:When 1, synergy is more notable.
9th, the missible oil containing copper rosinate and pyraclostrobin of the invention is to the Toxicity Determination of orange caterpillar：
The glass slide infusion process that experiment is recommended using FAO (Food and Agriculture Organization of the United Nation) (FAO).Every piece of glass slide be inoculated with 20 it is living
It is female to set series concentration value on the basis of 3 pilot studies into mite, clear water control, often handle 3 repetitions (totally 60 it is female into
Mite).Leaching medicine is blotted after 5 seconds with blotting paper immediately, is then placed in 24-26 DEG C of insulating box, is checked within 48 hours after medicine dead
Rate calculates corrected mortality.By concentration conversion into natural logrithm value, corrected mortality is converted into arcsine transformation value, then with anti-
Sinusoidal replacement value is dependent variable, and using dosage natural logrithm value as independent variable, linear regression point is carried out with DPS Biological Statistic Analysis Softwares
Analysis obtains the toxicity regression linear equation of each medicament.It is dead that correction is obtained according to the toxicity regression linear equation of each medicament
Rate arcsine transformation value (y) obtains EC by looking into antilogarithm table50Value, then according to the abundant methods of Sun Yun calculate medicament toxicity index and
Co-toxicity coefficient (CTC).
Survey toxicity index=standard agent EC50/ reagent agent EC50×100
Single dose theoretical toxicity index=content of the single dose actual measurement toxicity index × single dose in mixture
The sum of mixture theoretical toxicity index=each single dose theoretical toxicity index
Toxicity index/mixture theoretical toxicity index × 100 of co-toxicity coefficient=mixture actual measurement
Evaluation criterion：CTC≤80 are antagonism, and 80 ＜ CTC ＜ 120 are summation action, and CTC >=120 are synergistic effect.
Result of the test is as shown in table 12.
The toxicity index of 12 each medicament of table and the co-toxicity coefficient of pyraclostrobin copper rosinate
| Reagent agent | Quality proportioning | EC50(mg/kg) | Co-toxicity coefficient |
| Copper rosinate | -- | 7.88 | - |
| Pyraclostrobin | -- | 6.11 | - |
| Copper rosinate：Pyraclostrobin | 1：40 | 3.88 | 158.34 |
| Copper rosinate：Pyraclostrobin | 1：30 | 3.69 | 166.79 |
| Copper rosinate：Pyraclostrobin | 1：15 | 3.55 | 173.97 |
| Copper rosinate：Pyraclostrobin | 1：10 | 3.12 | 198.62 |
| Copper rosinate：Pyraclostrobin | 1：1 | 3.01 | 207.22 |
| Copper rosinate：Pyraclostrobin | 5：1 | 3.38 | 203.64 |
| Copper rosinate：Pyraclostrobin | 10：1 | 4.01 | 187.46 |
| Copper rosinate：Pyraclostrobin | 15：1 | 4.31 | 178.14 |
| Copper rosinate：Pyraclostrobin | 20：1 | 4.61 | 167.89 |
| Copper rosinate：Pyraclostrobin | 30：1 | 4.81 | 161.60 |
The result of the test of table 12 shows copper rosinate and pyraclostrobin in mass ratio 1:40~30:1 compounding, to citrus aphid
Worm has synergistic effect, especially matches 1:30~20:When 1, synergy is more notable.
10th, the missible oil containing copper rosinate and pyraclostrobin of the invention is to the Toxicity Determination of phyllocnistis citrella stainton：
The glass slide infusion process that experiment is recommended using FAO (Food and Agriculture Organization of the United Nation) (FAO).Every piece of glass slide be inoculated with 20 it is living
It is female to set series concentration value on the basis of 3 pilot studies into mite, clear water control, often handle 3 repetitions (totally 60 it is female into
Mite).Leaching medicine is blotted after 5 seconds with blotting paper immediately, is then placed in 24-26 DEG C of insulating box, is checked within 48 hours after medicine dead
Rate calculates corrected mortality.By concentration conversion into natural logrithm value, corrected mortality is converted into arcsine transformation value, then with anti-
Sinusoidal replacement value is dependent variable, and using dosage natural logrithm value as independent variable, linear regression point is carried out with DPS Biological Statistic Analysis Softwares
Analysis obtains the toxicity regression linear equation of each medicament.It is dead that correction is obtained according to the toxicity regression linear equation of each medicament
Rate arcsine transformation value (y) obtains EC by looking into antilogarithm table50Value, then according to the abundant methods of Sun Yun calculate medicament toxicity index and
Co-toxicity coefficient (CTC).
Survey toxicity index=standard agent EC50/ reagent agent EC50×100
Single dose theoretical toxicity index=content of the single dose actual measurement toxicity index × single dose in mixture
The sum of mixture theoretical toxicity index=each single dose theoretical toxicity index
Toxicity index/mixture theoretical toxicity index × 100 of co-toxicity coefficient=mixture actual measurement
Evaluation criterion：CTC≤80 are antagonism, and 80 ＜ CTC ＜ 120 are summation action, and CTC >=120 are synergistic effect.
Result of the test is as shown in table 13.
The toxicity index of 13 each medicament of table and the co-toxicity coefficient of pyraclostrobin copper rosinate
| Reagent agent | Quality proportioning | EC50(mg/kg) | Co-toxicity coefficient |
| Copper rosinate | -- | 7.91 | - |
| Pyraclostrobin | -- | 6.31 | - |
| Copper rosinate：Pyraclostrobin | 1:40 | 3.81 | 166.28 |
| Copper rosinate：Pyraclostrobin | 1:30 | 4.01 | 158.14 |
| Copper rosinate：Pyraclostrobin | 1:15 | 3.81 | 166.70 |
| Copper rosinate：Pyraclostrobin | 1:10 | 3.55 | 179.48 |
| Copper rosinate：Pyraclostrobin | 1:1 | 3.12 | 204.83 |
| Copper rosinate：Pyraclostrobin | 5:1 | 3.08 | 208.71 |
| Copper rosinate：Pyraclostrobin | 10:1 | 3.38 | 207.69 |
| Copper rosinate：Pyraclostrobin | 15:1 | 4.01 | 189.26 |
| Copper rosinate：Pyraclostrobin | 20:1 | 4.33 | 178.56 |
| Copper rosinate：Pyraclostrobin | 30:1 | 4.62 | 168.54 |
The result of the test of table 13 shows copper rosinate and pyraclostrobin in mass ratio 1:40~30:1 compounding, dives to citrus
Leaf moth has synergistic effect, especially matches 1:15~30:When 1, synergy is more notable.
11st, the missible oil containing copper rosinate and pyraclostrobin of the invention is to the Toxicity Determination of citrus aleyrodid：
The glass slide infusion process that experiment is recommended using FAO (Food and Agriculture Organization of the United Nation) (FAO).Every piece of glass slide be inoculated with 20 it is living
It is female to set series concentration value on the basis of 3 pilot studies into mite, clear water control, often handle 3 repetitions (totally 60 it is female into
Mite).Leaching medicine is blotted after 5 seconds with blotting paper immediately, is then placed in 24-26 DEG C of insulating box, is checked within 48 hours after medicine dead
Rate calculates corrected mortality.By concentration conversion into natural logrithm value, corrected mortality is converted into arcsine transformation value, then with anti-
Sinusoidal replacement value is dependent variable, and using dosage natural logrithm value as independent variable, linear regression point is carried out with DPS Biological Statistic Analysis Softwares
Analysis obtains the toxicity regression linear equation of each medicament.It is dead that correction is obtained according to the toxicity regression linear equation of each medicament
Rate arcsine transformation value (y) obtains EC by looking into antilogarithm table50Value, then according to the abundant methods of Sun Yun calculate medicament toxicity index and
Co-toxicity coefficient (CTC).
Survey toxicity index=standard agent EC50/ reagent agent EC50×100
Single dose theoretical toxicity index=content of the single dose actual measurement toxicity index × single dose in mixture
The sum of mixture theoretical toxicity index=each single dose theoretical toxicity index
Toxicity index/mixture theoretical toxicity index × 100 of co-toxicity coefficient=mixture actual measurement
Evaluation criterion：CTC≤80 are antagonism, and 80 ＜ CTC ＜ 120 are summation action, and CTC >=120 are synergistic effect.
Result of the test is as shown in table 14.
The toxicity index of 14 each medicament of table and the co-toxicity coefficient of pyraclostrobin copper rosinate
| Reagent agent | Quality proportioning | EC50(mg/kg) | Co-toxicity coefficient |
| Copper rosinate | -- | 8.25 | - |
| Pyraclostrobin | -- | 6.32 | - |
| Copper rosinate：Pyraclostrobin | 1:40 | 3.99 | 159.30 |
| Copper rosinate：Pyraclostrobin | 1:30 | 3.82 | 166.70 |
| Copper rosinate：Pyraclostrobin | 1:15 | 3.45 | 185.25 |
| Copper rosinate：Pyraclostrobin | 1:10 | 3.19 | 201.06 |
| Copper rosinate：Pyraclostrobin | 1:1 | 3.08 | 209.65 |
| Copper rosinate：Pyraclostrobin | 5:1 | 3.42 | 209.27 |
| Copper rosinate：Pyraclostrobin | 10:1 | 4.02 | 195.28 |
| Copper rosinate：Pyraclostrobin | 15:1 | 4.36 | 184.11 |
| Copper rosinate：Pyraclostrobin | 20:1 | 4.65 | 174.10 |
| Copper rosinate：Pyraclostrobin | 30:1 | 4.82 | 168.71 |
The result of the test of table 14 shows copper rosinate and pyraclostrobin in mass ratio 1:40~30:1 compounding, to citrus powder
Lice has synergistic effect, especially matches 1:30~30:When 1, synergy is more notable.
It can be seen that the present invention the missible oil containing copper rosinate and pyraclostrobin to citrus red mite, citrus rust mite,
Citrus resin disease, anthracnose of orange, citrus downy mildew, citrus ulcer, citrus gray mold and citrus whitefly aphid have significantly
Synergistic effect, control effect are good.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of missible oil containing copper rosinate and pyraclostrobin, which is characterized in that include the raw material of following parts by weight：Rosin
Sour copper 1-30 parts and 1-40 parts of pyraclostrobin.
2. a kind of missible oil containing copper rosinate and pyraclostrobin according to claim 1, which is characterized in that including as follows
The raw material of parts by weight：20 parts of 15 parts of copper rosinate and pyraclostrobin.
A kind of 3. missible oil containing copper rosinate and pyraclostrobin according to claim 1, which is characterized in that the missible oil
Also contain auxiliary agent；The ratio of the weight of the total weight and auxiliary agent of the copper rosinate and pyraclostrobin is 1：(0.8-1).
A kind of 4. missible oil containing copper rosinate and pyraclostrobin according to claim 3, which is characterized in that the auxiliary agent
Including one or both of dispersant, emulsifier, antifreezing agent, thickener, antifoaming agent, solvent, wetting agent, disintegrant, filler
Above mixture.
A kind of 5. missible oil containing copper rosinate and pyraclostrobin according to claim 4, which is characterized in that the auxiliary agent
Mixture for dispersant, emulsifier and solvent composition；The total weight of the copper rosinate and pyraclostrobin and dispersant, breast
Agent, the weight of solvent ratio be 1：(0.1-15)：(0.1-12)：(25.9-95.8).
6. any a kind of missible oil containing copper rosinate and pyraclostrobin according to claim 4 or 5, feature
It is, the dispersant is sodium lignin sulfonate, naphthalene sulfonic acid-formaldehyde condensation product sodium salt, APES formaldehyde condensation product
In sodium sulphate, alkyl phenol polyoxyethylene ether, sodium polycarboxylate, aliphatic amine polyoxyethylene ether and glycerin polyoxyethylene ether fatty ester
One or more kinds of mixtures.
7. any a kind of missible oil containing copper rosinate and pyraclostrobin according to claim 4 or 5, feature
It is, the emulsifier is alkyl phenol polyoxyethylene ether phosphate, polyoxyethylene aliphatic alcohol ether, styrylphenol polyoxyethylene
Ether, polyoxyethylene carboxylate sodium phosphate, calcium dodecyl benzene sulfonate, aliphatic acid polyethenoxy base ester, dodecyl polyoxy second
Alkene ether, dodecyl polyoxyethylene sulfonic acid sodium and phenethyl phenol poly-oxypropylene polyoxyethylene ether, polyvinyl alcohol, dodecylphenol gather
The mixture of one or more of ethylene oxide ether, lauryl sodium sulfate, Negel.
8. any a kind of missible oil containing copper rosinate and pyraclostrobin according to claim 4 or 5, feature
It is, the solvent is dimethylbenzene, diesel oil, benzene, cyclohexanol, isopropanol, soybean oil, turpentine-based vegetable oil, methyl oleate, solvent
The mixture of one or more of oil, rapeseed oil, ethyl alcohol, N- pyrrolidones, ethyl acetate and water.
9. a kind of preparation method of the missible oil containing copper rosinate and pyraclostrobin, includes the following steps：
Copper rosinate and pyraclostrobin are taken, the parts by weight of the copper rosinate are 1-30 parts, the weight of the pyraclostrobin
It is 1-40 parts to measure number, after mixing to get the missible oil containing copper rosinate and pyraclostrobin；
Or copper rosinate, pyraclostrobin and auxiliary agent are taken, the parts by weight of the copper rosinate are 1-30 parts, the pyrazoles ether bacterium
The parts by weight of ester are 1-40 parts, and the ratio of the weight of the total weight and auxiliary agent of the copper rosinate and pyraclostrobin is 1：
(0.8-1), after mixing to get the missible oil containing copper rosinate and pyraclostrobin.
It is 10. a kind of as red in prevention citrus such as missible oil of the claim 1-8 any one of them containing copper rosinate and pyraclostrobin
Spider, citrus rust mite, citrus resin disease, anthracnose of orange, citrus downy mildew, citrus ulcer, citrus gray mold and citrus
Application on aleyrodid aphid.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102217621A (en) * | 2011-07-18 | 2011-10-19 | 青岛瀚生生物科技股份有限公司 | Bactericide composition containing pyraclostrobin and copper rosinate |
| CN107410296A (en) * | 2017-06-22 | 2017-12-01 | 广东植物龙生物技术股份有限公司 | A kind of preparation method of pyraclostrobin and copper rosinate suspending agent |
-
2017
- 2017-12-28 CN CN201711461182.2A patent/CN108184881A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102217621A (en) * | 2011-07-18 | 2011-10-19 | 青岛瀚生生物科技股份有限公司 | Bactericide composition containing pyraclostrobin and copper rosinate |
| CN107410296A (en) * | 2017-06-22 | 2017-12-01 | 广东植物龙生物技术股份有限公司 | A kind of preparation method of pyraclostrobin and copper rosinate suspending agent |
Non-Patent Citations (1)
| Title |
|---|
| 蒲占湑等: "六种杀菌剂对柑橘炭疽病菌的室内毒力和田间防治效果", 《浙江农业学报》 * |
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Application publication date: 20180622 |