CN106508944A - Pesticidal composition containing 2-methylsulfonyl-5-(2,4-dichlorophenyl)-1,3,4-oxadiazole and prochloraz - Google Patents
Pesticidal composition containing 2-methylsulfonyl-5-(2,4-dichlorophenyl)-1,3,4-oxadiazole and prochloraz Download PDFInfo
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- CN106508944A CN106508944A CN201610791092.9A CN201610791092A CN106508944A CN 106508944 A CN106508944 A CN 106508944A CN 201610791092 A CN201610791092 A CN 201610791092A CN 106508944 A CN106508944 A CN 106508944A
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- Prior art keywords
- prochloraz
- bacterium azoles
- dichloro
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- dislikes bacterium
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/38—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< where at least one nitrogen atom is part of a heterocyclic ring; Thio analogues thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/82—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with three ring hetero atoms
Abstract
The invention discloses a pesticidal composition containing 2-methylsulfonyl-5-(2,4-dichlorophenyl)-1,3,4-oxadiazole and prochloraz. The composition contains active ingredients, and the active ingredients comprise 1-50 parts by weight of 2-methylsulfonyl-5-(2,4-dichlorophenyl)-1,3,4-oxadiazole and 1-50 parts by weight of prochloraz. The pesticidal composition can overcome and delay drug resistance of pathogens, the controlling effect of the pesticidal composition is better than that of a single drug, and the pesticidal composition is resistant to rain wash, good in fast-acting property, long in persistent period, reduced in usage frequency, timesaving, laborsaving, and capable of controlling a plurality of pests and diseases and reducing drug cost.
Description
Technical field
The invention belongs to pesticide field, and in particular to a kind of composition pesticide for disliking bacterium azoles and Prochloraz containing dichloro.
Background technology
Due to agricultural chemicals it is single using and medication not science, unreasonable, rapidly increased insect to currently used agriculture
The resistance of medicine so that some agricultural chemicals new components that high cost, long-time input are developed shorten service life, while access times
It is many, also increase environmental pollution and increase drug cost.Citrus psylla is unique medium that yellow twig is propagated, and has tender tip in the same year
In the case of, in 11～14 generations, can occur for 1 year, field generations are overlapped, and single wood louse passes sick rate and is up to 70-80%, and adult can fly,
Difficulty of prevention and cure is high.Meanwhile, in each growth period of oranges and tangerines, except wood louse, also red spider, blister mite, leaf miner, aleyrodid, aphid
Occur while the disease such as the insect pests such as worm and resin disease (rubber disease), anthracnose, so such pest and disease damage in the urgent need to address is asked
Topic.At present for the control of insect for being agriculturally also easy to produce resistance, main method is that exploitation is new with existing kind no interactions resistance
Compounding between composition or existing pesticide species, the former is as required development cost is high, the cycle is long, and development rate at present
The speed for much producing less than pest resistance to insecticide.
Dichloro dislikes bacterium azoles：It is a kind of azoles sulfone series bactericidal agent, chemical name is：2- methyl sulfuryl -5- (2,4 dichloro benzenes
Base) -1,3,4- diazole, molecular structure isIts molecular formula is C9H6Cl2O3S, average molecular
Quality is 293.1.It is a kind of new type bactericide, is low toxicity, the green chemical pesticide of safety, but the agricultural chemicals is not also extensively made
With.Physicochemical property：Active compound is acicular crystal, water insoluble, is soluble in conventional organic solvent.Active compound to light, thermally-stabilised, in alkalescence
Easily decompose under condition (pH10).Mammalian toxicity：Rat acute LD50=1780mg/kg.Rat acute is percutaneous and sucks
LD50>2000mg/kg。
Prochloraz, chemical name：N- propyl group-N- [2- (2,4,6- Trichlorophenoxies) ethyl]-imidazoles -1- formamides.Point
Minor：C15H16Cl3N3O2, molecular weight：376.7, stability：It is stable in 20 DEG C, the water of pH7, to concentrated acid or alkali and sunlight not
It is stable.Degrade in the sunlight, it is stable under normal shelf conditions, it is unstable under strong acid, basic conditions, it is mainly used in preventing and treating mandarin orange
The blossom-end rot of tangerine fruit storage phase, penicilliosis, green mould, anthracnose.
Above-mentioned citrus red mite, oranges and tangerines blister mite, oranges and tangerines resin disease, citrus anthracnose, oranges and tangerines downy mildew, c itrus canker
Disease, oranges and tangerines gray mold, orange caterpillar, diaphorina citri generate certain resistance to the action of a drug to Prochloraz, and exclusive use has not reached reason
The prevention effect thought.At present, we not yet find and for dichloro to dislike Patents and the document report that bacterium azoles and Prochloraz are applied in combination
Road.
The content of the invention
The purpose of the present invention solves above-mentioned technical problem, there is provided a kind of pesticide combination for disliking bacterium azoles and Prochloraz containing dichloro
Thing, can overcome and delay that the pathogen resistance to the action of a drug, preventive effect be good better than single dose, resistance of rainwater washing against, quick-acting, the lasting period is long, reduce
Access times, save time, laborsaving, can prevent and treat various pests and disease simultaneously, reduce drug cost.
For realizing above-mentioned purpose, the technical scheme is that：
A kind of composition pesticide for disliking bacterium azoles and Prochloraz containing dichloro, including active component, the active component is dichloro
Bacterium azoles and Prochloraz is disliked, is calculated by weight as：
Dichloro dislikes bacterium azoles 1-50 parts；
Prochloraz 1-50 parts.
Used as further technical scheme, it is 1-5 parts that the above dichloro dislikes bacterium azoles, and the Prochloraz is 1-50 parts.
Used as further technical scheme, it is 1-10 parts that the above dichloro dislikes bacterium azoles, and the Prochloraz is 1-30 parts.
Used as further technical scheme, it is 1-20 parts that the above dichloro dislikes bacterium azoles, and the Prochloraz is 1-30 parts.
Used as further technical scheme, it is 1-30 parts that the above dichloro dislikes bacterium azoles, and the Prochloraz is 1-30 parts.
Used as further technical scheme, it is 1-30 parts that the above dichloro dislikes bacterium azoles, and the Prochloraz is 1-20 parts.
Used as further technical scheme, above composition pesticide also includes auxiliary agent, and the auxiliary agent includes dispersant, emulsification
One or more in agent, antifreezing agent, thickener, defoamer, solvent, wetting agent, disintegrant and filler.
Used as further technical scheme, the parts by weight of the above auxiliary agent addition are：
Used as further technical scheme, the above emulsifying agent is calcium dodecyl benzene sulfonate, dodecyl polyoxy second
Alkene ether, styrylphenol polyoxyethylene ether, NPE, thermally coupled distillation columns, fatty acid polyglycol
In oxyethylene group ester, polyoxyethylene aliphatic alcohol ether, dodecyl polyoxyethylene sulfonic acid sodium salt and phenethyl phenol polyethenoxy ether
One or more combination；
The above solvent is dimethylbenzene, diesel oil, toluene, cyclohexanol, methyl alcohol, soybean oil, turpentine oil, methyl oleate, molten
One or more combination in agent oil, dimethylformamide, ethanol, N- pyrrolidones, ethyl acetate and water；
The above wetting agent is lauryl sodium sulfate, calcium dodecyl benzene sulfonate, the condensation of methyl naphthalene sulfonic acid sodium formaldehyde
One or more combination in thing, pull open powder, Negel and spaonin powder；
The above dispersant is sodium lignin sulfonate, calcium lignosulfonate, APES formaldehyde condensation product
Sodium sulphate, calcium dodecyl benzene sulfonate, APES, polycarboxylic acids sodium, aliphatic amine polyoxyethylene ether and glycerine polyoxy
One or more combination in ethene ether fatty acid ester；
The above antifreezing agent is one or more the combination in ethylene glycol, propane diols, glycerine and urea；
The above thickener be xanthans, Magnesiumaluminumsilicate, hydroxymethyl cellulose and polyvinyl alcohol in one or two
Combination above；
The above defoamer is one or more the group in silicone oil, organic silicone and polypropylene glycerol aether
Close；
The above disintegrant be bentonite, ammonium sulfate, sodium acid carbonate, aluminium chloride and succinic acid in one or two with
On combination；
The above filler be white carbon, kaolin, diatomite, precipitated calcium carbonate and starch in one or more
Combination.
As further technical scheme, the formulation of above composition pesticide be suspending agent, aqueous emulsion, water dispersible granules,
Microemulsion or wettable powder.
A kind of preparation method of composition pesticide for disliking bacterium azoles and Prochloraz containing dichloro as above, it be by activity into
Divide and auxiliary agent mixes by weight, be obtained by the conventional machining mode of its formulation.
A kind of purposes of composition pesticide for disliking bacterium azoles and Prochloraz containing dichloro as above, it is red in preventing and treating oranges and tangerines
Spider, oranges and tangerines blister mite, oranges and tangerines resin disease, citrus anthracnose, oranges and tangerines downy mildew, citrus bacterial canker disease, oranges and tangerines gray mold, oranges and tangerines aphid
Application in worm, diaphorina citri.
The component of the present invention is commercially available prod.
Compared with prior art, the device have the advantages that being：Prochloraz in the present invention due to time of application compared with
It is early, citrus red mite, oranges and tangerines blister mite, oranges and tangerines resin disease, citrus anthracnose, oranges and tangerines downy mildew, citrus bacterial canker disease, oranges and tangerines grey mold
Disease, orange caterpillar, diaphorina citri have generated stronger drug resistance to which；Dichloro dislike bacterium azoles to soil-borne disease and migrate disease and
Other fungal diseases have good inhibiting effect, it has been investigated that, dichloro dislikes bacterium azoles and Prochloraz with mass ratio 1:50～50:
1 compounding, can produce higher synergistic effect, expand broad-spectrum, overcome and delay pathogen to develop immunity to drugs.Preventive effect is better than single
Agent, resistance of rainwater washing against, quick-acting are good, the lasting period is long, reduce access times, save time, laborsaving, various pathogens can be prevented and treated simultaneously with
And disease, reduce drug cost.Especially to citrus red mite, oranges and tangerines blister mite, oranges and tangerines resin disease, citrus anthracnose, oranges and tangerines
Downy mildew, citrus bacterial canker disease, oranges and tangerines gray mold, orange caterpillar, diaphorina citri have obvious synergistic effect, and prevention effect is good.
Specific embodiment
With reference to embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited to
The scope that embodiment is represented.
Embodiment 1：50% Prochloraz dichloro dislikes bacterium azole suspending agent
Embodiment 2：2% Prochloraz dichloro dislikes bacterium azole suspending agent
Embodiment 3：60% Prochloraz dichloro dislikes bacterium azole suspending agent
Embodiment 4：60% Prochloraz dichloro dislikes bacterium azole suspending agent
Embodiment 5：30% Prochloraz dichloro dislikes bacterium azole suspending agent
The preparation method of embodiment 1-5 is：Proportioning according to each embodiment is weighed after raw material, and raw material, solvent mixing are stirred
Dissolving is mixed, adds the auxiliary agents such as emulsification, dispersion to carry out high speed shearing emulsification, made corresponding dichloro and dislike the suspension of bacterium azoles Prochloraz
Agent.
Embodiment 6：40% Prochloraz dichloro dislikes bacterium azoles aqueous emulsion
Embodiment 7：2% Prochloraz dichloro dislikes bacterium azoles aqueous emulsion
Embodiment 8：60% Prochloraz dichloro dislikes bacterium azoles aqueous emulsion
Embodiment 9：60% Prochloraz dichloro dislikes bacterium azoles aqueous emulsion
Embodiment 10：30% Prochloraz dichloro dislikes bacterium azoles aqueous emulsion
The preparation method of embodiment 6-10 is：Proportioning according to each embodiment is weighed after raw material, and raw material, solvent mixing are stirred
Dissolving is mixed, adds emulsifying agent to carry out high speed shearing emulsification, made corresponding Prochloraz dichloro and dislike bacterium azoles aqueous emulsion.
Embodiment 11：25% Prochloraz dichloro dislikes bacterium azoles water dispersible granules
Embodiment 12：2% Prochloraz dichloro dislikes bacterium azoles water dispersible granules
Embodiment 13：55% Prochloraz dichloro dislikes bacterium azoles water dispersible granules
Embodiment 14：60% Prochloraz dichloro dislikes bacterium azoles water dispersible granules
Embodiment 15：40% Prochloraz dichloro dislikes bacterium azoles water dispersible granules
The preparation method of embodiment 11-15 is：Proportioning according to each embodiment is weighed after raw material, and raw material mixed powder is broken,
Corresponding Prochloraz dichloro is made through granulation, drying and dislikes bacterium azoles water dispersible granules.
Embodiment 16：30% Prochloraz dichloro dislikes bacterium azoles wettable powder
Embodiment 17：2% Prochloraz dichloro dislikes bacterium azoles wettable powder
Embodiment 18：60% Prochloraz dichloro dislikes bacterium azoles wettable powder
Embodiment 19：55% Prochloraz dichloro dislikes bacterium azoles wettable powder
Embodiment 20：40% Prochloraz dichloro dislikes bacterium azoles wettable powder
The preparation method of embodiment 16-20 is：Proportioning according to each embodiment is weighed after raw material, by raw material mixing Ultramicro-powder
It is broken, make corresponding Prochloraz dichloro and dislike bacterium azoles wettable powder.
Embodiment 21：40% Prochloraz dichloro dislikes bacterium azoles microemulsion
Embodiment 22：2% Prochloraz dichloro dislikes bacterium azoles microemulsion
Embodiment 23：60% Prochloraz dichloro dislikes bacterium azoles microemulsion
Embodiment 24：55% Prochloraz dichloro dislikes bacterium azoles microemulsion
Embodiment 25：30% Prochloraz dichloro dislikes bacterium azoles microemulsion
The preparation method of embodiment 20-25 is：Proportioning according to each embodiment is weighed after raw material, and raw material, solvent are mixed
Stirring and dissolving, adds emulsifying agent, water to carry out high speed shear, makes corresponding Prochloraz dichloro and dislikes bacterium azoles microemulsion.
Application Example：
In order to detect the insecticidal effect of the composition of the present invention, following experimental data is now enumerated：
Table 1 below to table 10 medicament dilution after fountain height by the conventional spray requirement of this area, i.e. leaf just
The back side, fruit surface are sprayed to and to form drop-wise and flow down.
Application Example 5
30% Prochloraz dichloro that the embodiment of the present invention 5 is obtained dislike bacterium azole suspending agent and 25% Prochloraz missible oil,
20% dichloro is disliked bacterium azole suspending agent and carries out field control effectiveness test, and test site is Guilin City Guangxi land reclamation and cultivation mono- group of navel orange of Guilin Liang Feng
Orchard, spray-on process adopt double dilution method, uniformly spray in oranges and tangerines branches and leaves and fruit during preparating liquid, in the morbidity of resin disease just
Phase carries out first time dispenser, applies altogether 4 times, is investigated within 20 days after last medicine, and investigation area is calculated (per cell as 2～3 plants with cell
Mandarin tree), investigate 2 plants of trees per cell, per plant by east, south, west, north, in the sampling of five points, two branches of per investigation, each
Branch investigates top down 4 leaves and whole fruits, records total leaf (fruit) number, disease leaf (fruit) number and the sick level of investigation, calculates sick
Feelings index and preventive effect.
Preventing and treating oranges and tangerines resin disease (rubber disease) the result of the test such as table 1 of Application Example 5：
1 30% Prochloraz dichloro of table dislikes bacterium azole suspending agent preventing and treating oranges and tangerines resin disease (rubber disease) test of pesticide effectiveness result
1 result of the test of table shows, starts to apply first time medicine in oranges and tangerines resin disease (rubber disease) early period of origination, thanking to colored back seat
Fruit middle and later periods, young fruit period, fruit growth period are respectively applied once again, and 30% Prochloraz dichloro dislikes bacterium azole suspending agent preventing and treating oranges and tangerines resin
Disease (rubber disease) has preferable preventive effect by 1500 times of dilution sprayings.
Application Example 7
2% Prochloraz dichloro that the embodiment of the present invention 7 is obtained dislike bacterium azoles aqueous emulsion and 25% Prochloraz missible oil, 20%
Dichloro is disliked bacterium azole suspending agent and carries out field control effectiveness test, and test site is mono- group of navel orange fruit of Guilin City Guangxi land reclamation and cultivation Guilin Liang Feng
Garden, foliar spray method are watered dilution by process dosage and are made into liquid, uniformly spray in oranges and tangerines leaf tow sides, in oranges and tangerines
Aphid full incidence period dispenser 1 time, investigates insect population shortly before dispenser, 1 day after dispenser, respectively investigate borer population of once surviving within 3 days, 7 days, altogether
3 times.Investigation area with cell calculate (per cell as 2～3 plants of mandarin trees), per cell investigate 2 mandarin trees, per plant press east, south,
West, north, in 5 orientation investigate 5 killed tips, investigate the aphid number of 5 leaves of top tip per the tip, calculate relative control effect.
7 doses of preventing and treating orange caterpillar result of the test such as tables 2 of Application Example：
2 2% Prochloraz dichloro of table dislikes bacterium azoles aqueous emulsion preventing and treating orange caterpillar test of pesticide effectiveness result
2 result of the test of table shows that, in mandarin tree aphid full incidence period dispenser, 2% Prochloraz dichloro is disliked bacterium azoles aqueous emulsion and pressed
Amount spray used above, has preferable preventive effect to preventing and treating orange caterpillar.
Application Example 15
The 40% Prochloraz dichloro that the embodiment of the present invention 15 is obtained dislikes bacterium azoles water dispersible granules and 25% Prochloraz breast
Oil, 20% dichloro are disliked bacterium azole suspending agent and carry out field control effectiveness test, and test site is Guilin City Guangxi land reclamation and cultivation mono- teams of Guilin Liang Feng
Navel orange orchard, foliar spray method are watered dissolved dilution by process dosage and are made into liquid, the whole strain of mandarin tree is sprayed,
Orange young is taken out and sends out phase dispenser 1 time, investigates insect population shortly before dispenser, 5 days after dispenser, respectively investigates within 10 days, 15 days and once survives
Borer population, totally 3 times.Investigation area is calculated (per cell as 2～3 plants of mandarin trees) with cell, and investigation method determines tip investigation using singling, often
Cell investigates 2 mandarin trees, per plant by east, south, west, north, in 5 different azimuths respectively choose 2 and have the worm tip to be marked, investigate
Record work borer population per the tip.Compare with blank district, calculate relative control effect.
Application Example 15 prevents and treats diaphorina citri result of the test such as table 3：
3 40% Prochloraz dichloro of table is disliked bacterium azoles water dispersible granules and prevents and treats diaphorina citri test of pesticide effectiveness result
3 result of the test of table shows that endanger phase dispenser in diaphorina citri, 40% Prochloraz dichloro dislikes bacterium azoles water dispersible granules
In the case of amount used above, there is preferable preventive effect to preventing and treating diaphorina citri.
The 40% Prochloraz dichloro that the embodiment of the present invention 15 is obtained dislikes bacterium azoles water dispersible granules and 25% Prochloraz breast
Oil, 20% dichloro are disliked bacterium azole suspending agent and carry out field control effectiveness test, and test site is Guilin City Guangxi land reclamation and cultivation mono- teams of Guilin Liang Feng
Navel orange orchard, foliar spray method are watered dilution by process dosage and are made into liquid, uniformly spray in oranges and tangerines leaf tow sides,
In orange caterpillar full incidence period dispenser 1 time, insect population shortly before dispenser, is investigated, 1 day after dispenser, respectively investigate within 3 days, 7 days and once survive
Borer population, totally 3 times.Investigation area calculates (per cell as 2～3 plants of mandarin trees) with cell, investigates 2 mandarin trees per cell, and per plant is pressed
East, south, west, north, in 5 orientation investigate 5 killed tips, investigate the aphid number of 5 leaves of top tip per the tip, calculate relative control effect.
Application Example 15 prevents and treats orange caterpillar result of the test such as table 4：
4 40% Prochloraz dichloro of table dislikes bacterium azoles water dispersible granules preventing and treating orange caterpillar test of pesticide effectiveness result
4 result of the test of table shows that, in orange caterpillar full incidence period dispenser, 40% Prochloraz dichloro dislikes bacterium azoles water dispersible granules
In the case of amount used above, there is preferable preventive effect to preventing and treating orange caterpillar.
Application Example 16
The 30% Prochloraz dichloro that the embodiment of the present invention 16 is obtained dislikes bacterium azoles wettable powder and 25% Prochloraz breast
Oil, 20% dichloro are disliked bacterium azole suspending agent and carry out field control effectiveness test, and test site is Guilin City Guangxi land reclamation and cultivation mono- teams of Guilin Liang Feng
Navel orange orchard, foliar spray method are watered dilution by process dosage and are made into liquid, uniformly spray in mandarin tree blade, branch extremely
Moistening, in oranges and tangerines blister mite peak of occurrence early stage dispenser 1 time, investigates mite quantity living shortly before dispenser, 1 day after dispenser, 7 days, 14 days,
21 days, 28 days each mite numbers remaining living for investigating once fixed point leaf, totally 5 times.Investigation area is calculated (per cell as 2～3 plants of mandarin oranges with cell
Tangerine), investigation method is that every cell investigates 2 mandarin trees, per plant by east, south, west, north, in 5 azimuth mark side shoots, investigation
The mite quantity living of 20 new leaf backs, directly observes the mite number in each visual field with 10 times of folding hand held magnascopes.It is right with blank
Compare according to area, calculate relative control effect.
Application Example 16 prevents and treats oranges and tangerines blister mite result of the test such as table 5：
5 30% Prochloraz dichloro of table dislikes bacterium azoles wettable powder preventing and treating oranges and tangerines blister mite test of pesticide effectiveness result
5 result of the test of table shows, in oranges and tangerines blister mite peak of occurrence early stage dispenser, 30% Prochloraz dichloro is disliked bacterium azoles and existed
In the case of amount used above, there is preferable preventive effect to wettable powder preventing and treating oranges and tangerines blister mite.
The 30% Prochloraz dichloro that the embodiment of the present invention 16 is obtained dislikes bacterium azoles wettable powder and 25% Prochloraz breast
Oil, 20% dichloro are disliked bacterium azole suspending agent and carry out field control effectiveness test, and test site is Guilin City Guangxi land reclamation and cultivation mono- teams of Guilin Liang Feng
Navel orange orchard, foliar spray method are watered dilution by process dosage and are made into liquid, uniformly spray in mandarin tree blade positive and negative two
Face, limb are contained in citrus red mite and send out initial stage dispenser 1 time to moistening, and investigate mite quantity living shortly before dispenser, 15 days after dispenser, 20
My god, each investigation in 30 days once pinpoint the remaining red spider quantity of leaf, totally 3 times.Investigation area is calculated (per cell as 2～3 plants with cell
Mandarin tree), investigation method is that every cell investigates 2 mandarin trees, tree crown by east, south, west, north, in each mark in 5 orientation one
Treetop branch, 5 blades that investigation is most pushed up per the tip investigate remaining red spider quantity on 25 blades, altogether with 10 times of collapsible hands
Hold the mite number that magnascope directly observes each visual field.Compare with blank district, calculate relative control effect.
Application Example 16 prevents and treats citrus red mite result of the test such as table 6：
6 30% Prochloraz dichloro of table dislikes bacterium azoles wettable powder preventing and treating citrus red mite test of pesticide effectiveness result
6 result of the test of table shows that containing in citrus red mite and sending out initial stage dispenser, 30% Prochloraz dichloro dislikes bacterium azoles wettable
Pulvis has preferable preventive effect to preventing and treating citrus red mite in the case of amount used above.
Application Example 22
2% Prochloraz dichloro that the embodiment of the present invention 22 is obtained dislike bacterium azoles microemulsion and 25% Prochloraz missible oil,
20% dichloro is disliked bacterium azole suspending agent and carries out field control effectiveness test, and test site is Guilin City Guangxi land reclamation and cultivation mono- group of navel orange of Guilin Liang Feng
Orchard, foliar spray method are watered dissolved dilution by process dosage and are made into liquid, the whole strain of mandarin tree sprayed, in oranges and tangerines
Young sprout is taken out and sends out phase dispenser 1 time, investigates insect population shortly before dispenser, 5 days after dispenser, respectively investigates borer population of once surviving within 10 days, 15 days,
Totally 3 times.Investigation area is calculated (per cell as 2～3 plants of mandarin trees) with cell, and investigation method determines tip investigation using singling, per cell
2 mandarin trees of investigation, per plant by east, south, west, north, in 5 different azimuths respectively choose 2 and have the worm tip to be marked, investigation records
The work borer population per the tip.Compare with blank district, calculate relative control effect.
22 doses of Application Example prevents and treats diaphorina citri result of the test such as table 7：
7 2% Prochloraz dichloro of table is disliked bacterium azoles microemulsion and prevents and treats diaphorina citri test of pesticide effectiveness result
7 result of the test of table shows, causes harm phase dispenser in diaphorina citri, 2% Prochloraz dichloro dislike bacterium azoles microemulsion with
In the case of upper usage amount, there is preferable preventive effect to preventing and treating diaphorina citri.
Application Example 6
40% Prochloraz dichloro that the embodiment of the present invention 6 is obtained dislike bacterium azoles aqueous emulsion and 25% Prochloraz missible oil,
20% dichloro is disliked bacterium azole suspending agent and carries out field control effectiveness test, and test site is Guilin City Guangxi land reclamation and cultivation mono- group of navel orange of Guilin Liang Feng
Orchard, using spray-on process, adopts double dilution method, uniformly sprays in oranges and tangerines branches and leaves and fruit, send out in downy mildew during preparating liquid
The sick initial stage carries out first time dispenser, applies 4 times altogether.Investigated within 20 days after last medicine.Investigation area is calculated (per cell as 2 with cell
～3 plants of mandarin trees), investigate 2 plants of trees per cell, per plant by east, south, west, north, in the sampling of five points, two branches of per investigation,
Each branch investigates top down 4 leaves and whole fruits, records total leaf (fruit) number, disease leaf (fruit) number and the sick level of investigation, meter
Calculate disease index and preventive effect.
Application Example 6 prevents and treats oranges and tangerines downy mildew result of the test such as table 8：
8 40% Prochloraz dichloro of table dislikes bacterium azoles aqueous emulsion preventing and treating oranges and tangerines downy mildew test of pesticide effectiveness result
8 result of the test of table shows, starts to apply first time medicine in oranges and tangerines downy mildew early period of origination, thank spend after in setting after
Phase, young fruit period, fruit growth period are respectively applied once again, and 40% Prochloraz dichloro dislikes what bacterium azoles aqueous emulsion was tested in amount used above
In the case of, there is preferable preventive effect to preventing and treating oranges and tangerines downy mildew.
40% Prochloraz dichloro that the embodiment of the present invention 6 is obtained dislike bacterium azoles aqueous emulsion and 25% Prochloraz missible oil,
20% dichloro is disliked bacterium azole suspending agent and carries out field control effectiveness test, and test site is Guilin City Guangxi land reclamation and cultivation mono- group of navel orange of Guilin Liang Feng
Orchard, using spray-on process, adopts double dilution method, uniformly sprays in oranges and tangerines branches and leaves and fruit, in oranges and tangerines anthrax during preparating liquid
Sick early stage or premorbid carry out first time dispenser, apply altogether 3 times, and dispenser is spaced 10 days, carry out within 15 days after last medicine disposable
Investigation, investigation area calculate (per cell as 2～3 plants of mandarin trees) with cell.
Blade disease survey method：Investigate 2 plants of trees per cell, per plant by east, south, west, north, in the sampling of five points, per point
Whole blades of two tips of investigation, record total leaf tree of investigation, sick leaf tree and sick level, calculate disease index and preventive effect.
Fruit disease investigation method：Two plants of mandarin trees, 100 fruits of every plant of random searching, record investigation is investigated per cell
Total fruit tree and disease fruit number, calculate diseased fruit rate and preventive effect.
Application Example 6 prevents and treats citrus anthracnose result of the test such as table 9：
9 40% Prochloraz dichloro of table dislikes bacterium azoles aqueous emulsion preventing and treating citrus anthracnose test of pesticide effectiveness result
9 result of the test of table shows, carries out first time dispenser, young fruit period, fruit in citrus anthracnose early stage or premorbid
Grow directly from seeds, 40% Prochloraz dichloro dislikes bacterium azoles aqueous emulsion in the case of the test of above dosage, to anti-
Controlling citrus anthracnose has preferable preventive effect.
Application Example 11
The 25% Prochloraz dichloro that the embodiment of the present invention 11 is obtained dislikes bacterium azoles water dispersible granules and 25% Prochloraz breast
Oil, 20% dichloro are disliked bacterium azole suspending agent and carry out field control effectiveness test, and test site is Guilin City Guangxi land reclamation and cultivation mono- teams of Guilin Liang Feng
Navel orange orchard, foliar spray method are watered dilution by process dosage and are made into liquid, uniformly spray in mandarin tree blade, branch extremely
Moistening, in oranges and tangerines blister mite peak of occurrence early stage dispenser 1 time, investigates mite quantity living shortly before dispenser, 1 day after dispenser, 7 days, 14 days,
21 days, 28 days each mite numbers remaining living for investigating once fixed point leaf, totally 5 times.Investigation area is calculated (per cell as 2～3 plants of mandarin oranges with cell
Tangerine), investigation method is that every cell investigates 2 mandarin trees, per plant by east, south, west, north, in 5 azimuth mark side shoots, investigation
The mite quantity living of 20 new leaf backs, directly observes the mite number in each visual field with 10 times of folding hand held magnascopes.It is right with blank
Compare according to area, calculate relative control effect.
Application Example 11 prevents and treats oranges and tangerines blister mite result of the test such as table 10：
10 25% Prochloraz dichloro of table dislikes bacterium azoles water dispersible granules preventing and treating oranges and tangerines blister mite test of pesticide effectiveness result
10 result of the test of table shows that, in oranges and tangerines blister mite peak of occurrence early stage dispenser, 25% Prochloraz dichloro dislikes bacterium azoles
Water dispersible granules have preferable preventive effect to preventing and treating oranges and tangerines blister mite in the case of amount used above test.
Application example embodiment 14
The 60% Prochloraz dichloro that the embodiment of the present invention 14 is obtained dislikes bacterium azoles water dispersible granules and 25% Prochloraz breast
Oil, 20% dichloro are disliked bacterium azole suspending agent and carry out field control effectiveness test, and test site is Guilin City Guangxi land reclamation and cultivation mono- teams of Guilin Liang Feng
Navel orange orchard, using spray-on process, adopts double dilution method, uniformly sprays in oranges and tangerines branches and leaves and fruit, in oranges and tangerines during preparating liquid
Canker early stage or premorbid carry out first time dispenser, apply altogether 3 times, and dispenser is spaced 10 days, carries out one within 15 days after last medicine
Secondary sex investigation, investigation area calculate (per cell as 2～3 plants of mandarin trees) with cell.
Blade disease survey method：Investigate 2 plants of trees per cell, per plant by east, south, west, north, in the sampling of five points, per point
Whole blades of two tips of investigation, record total leaf tree of investigation, sick leaf tree and sick level, calculate disease index and preventive effect.
Fruit disease investigation method：Two plants of mandarin trees, 100 fruits of every plant of random searching, record investigation is investigated per cell
Total fruit tree and disease fruit number, calculate diseased fruit rate and preventive effect.
Application Example 14 prevents and treats citrus bacterial canker disease result of the test such as table 11：
11 60% Prochloraz dichloro of table dislikes bacterium azoles aqueous emulsion preventing and treating citrus bacterial canker disease test of pesticide effectiveness result
11 result of the test of table shows, carries out first time dispenser, young fruit period, fruit in citrus bacterial canker disease early stage or premorbid
Grow directly from seeds, 60% Prochloraz dichloro dislikes bacterium azoles water dispersible granules in the case of the test of above dosage,
There is preferable preventive effect to preventing and treating citrus bacterial canker disease.
Application example embodiment 19
The 55% Prochloraz dichloro that the embodiment of the present invention 19 is obtained dislikes bacterium azoles wettable powder and 25% Prochloraz breast
Oil, 20% dichloro are disliked bacterium azole suspending agent and carry out field control effectiveness test, and test site is Guilin City Guangxi land reclamation and cultivation mono- teams of Guilin Liang Feng
Navel orange orchard, using spray-on process, adopts double dilution method, uniformly sprays in oranges and tangerines branches and leaves and fruit, in oranges and tangerines during preparating liquid
Gray mold early stage or premorbid carry out first time dispenser, apply altogether 3 times, and dispenser is spaced 10 days, carries out one within 15 days after last medicine
Secondary sex investigation, investigation area calculate (per cell as 2～3 plants of mandarin trees) with cell.
Blade disease survey method：Investigate 2 plants of trees per cell, per plant by east, south, west, north, in the sampling of five points, per point
Whole blades of two tips of investigation, record total leaf tree of investigation, sick leaf tree and sick level, calculate disease index and preventive effect.
Fruit disease investigation method：Two plants of mandarin trees, 100 fruits of every plant of random searching, record investigation is investigated per cell
Total fruit tree and disease fruit number, calculate diseased fruit rate and preventive effect.
Application Example 19 prevents and treats oranges and tangerines gray mold result of the test such as table 12：
12 55% Prochloraz dichloro of table dislikes bacterium azoles wettable powder preventing and treating oranges and tangerines gray mold test of pesticide effectiveness result
12 result of the test of table shows, carries out first time dispenser, young fruit period, fruit in oranges and tangerines gray mold early stage or premorbid
Grow directly from seeds, 55% Prochloraz dichloro dislikes bacterium azoles wettable powder in the case of the test of above dosage,
There is preferable preventive effect to preventing and treating oranges and tangerines gray mold.
Following instance illustrates the synergistic effect of composition
Toxicity Determination of the pesticide sterilizing Pesticidal combination to diaphorina citri
Subjects are diaphorina citri.
Test adopts medicine embrane method.Take cylindrical transparent glass preserving jar (base diameter 6.8cm, bottleneck diameter 5.5cm, height
8.7cm), add 50ml to be diluted to the liquid of desired concn, cover tightly lid, gently shake up, be inverted 30s, a bottle inwall is all glued
Then liquid is poured out, then allows bottle to dry naturally by full liquid.Oranges and tangerines tender shoots is soaked into 1min with identical liquid, is dried in the air naturally
It is put in the bottle for having dried after dry；Tender shoots base portion moisturizing is encased with the cotton of dipped identical liquid.Wood louse adult band plug glass
Test tube is gathered from kamuning tree.Each process is repeated 3 times, and repeats 10 adults every time.CO is used during process2Anesthesia (18-20s)
After be put into each process bottle in, blank clear water, each bottleneck are covered with 40 mesh gauzes, in case adult flies out.20 minutes
After check whether there is dead worm, borer population living before record medicine.The vial after worm will be connect it will be placed in the illumination box of temperature (25 ± 1) DEG C
In.After medicine, 24h estimates dead borer population, borer population living, calculates the death rate, and the clear water control death rate is efficiency test less than 10%.
First by concentration conversion into natural logrithm value, corrected mortality is converted into arcsine transformation value.Again with arcsine transformation
Be worth for dependent variable, with dosage natural logrithm value as independent variable, linear regression analysis carried out with DPS Biological Statistic Analysis Softwares, draw each
The toxicity regression linear equation of individual medicament.Toxicity index and the co-toxicity coefficient (CTC) of medicament are calculated according to the abundant methods of Sun Yun.
Actual measurement toxicity index=standard agent EC50/ reagent agent EC50×100
Single dose theoretical toxicity index=single dose actual measurement content of the toxicity index × single dose in mixture
Mixture theoretical toxicity index=each single dose theoretical toxicity index sum
Toxicity index/mixture theoretical toxicity index × 100 of co-toxicity coefficient=mixture actual measurement
Evaluation criterion：CTC≤80 are antagonism, and 80 ＜ CTC ＜ 120 are summation action, and CTC >=120 are synergistic effect.
Experimental result such as table 13：
Table 13：The toxicity index of each medicament and Prochloraz dichloro dislike the co-toxicity coefficient of bacterium azoles
| Reagent agent | Proportioning | EC50(mg/kg) | Co-toxicity coefficient |
| Prochloraz | -- | 5.76 | - |
| Dichloro dislikes bacterium azoles | -- | 4.25 | - |
| Prochloraz：Dichloro dislikes bacterium azoles | 50：1 | 3.52 | 162.5 |
| Prochloraz：Dichloro dislikes bacterium azoles | 40：1 | 3.39 | 168.45 |
| Prochloraz：Dichloro dislikes bacterium azoles | 30：1 | 3.21 | 177.41 |
| Prochloraz：Dichloro dislikes bacterium azoles | 15：1 | 3.01 | 187.21 |
| Prochloraz：Dichloro dislikes bacterium azoles | 1：1 | 2.59 | 188.85 |
| Prochloraz：Dichloro dislikes bacterium azoles | 1：5 | 2.64 | 168.34 |
| Prochloraz：Dichloro dislikes bacterium azoles | 1：10 | 2.74 | 155.96 |
| Prochloraz：Dichloro dislikes bacterium azoles | 1：15 | 2.81 | 153.76 |
| Prochloraz：Dichloro dislikes bacterium azoles | 1：20 | 2.93 | 146.88 |
| Prochloraz：Dichloro dislikes bacterium azoles | 1：30 | 3.06 | 140.07 |
| Prochloraz：Dichloro dislikes bacterium azoles | 1：40 | 3.34 | 128.069 |
| Prochloraz：Dichloro dislikes bacterium azoles | 1：50 | 3.39 | 126.02 |
Table 13 shows that Prochloraz dislikes bacterium azoles in mass ratio 50 with dichloro：1～1：50 compoundings, have synergy to make diaphorina citri
With especially proportioning is 1:15～50:When between 1, synergy is more notable, and co-toxicity coefficient is all higher than 150, is far longer than synergistic effect
Evaluation criterion 120.
Toxicity Determination of the composition of the present invention to citrus red mite：
The slide infusion process that test is recommended using FAO (Food and Agriculture Organization of the United Nation) (FAO).Every piece of slide be inoculated with 20 it is living
It is female to set series concentration value on the basis of 3 pilot studies into mite, clear water control, often process 3 repetitions (totally 60 it is female into
Mite).Leaching medicine is blotted with blotting paper after 5 seconds immediately, is then placed in 24-26 DEG C of insulating box, checks dead within 48 hours after medicine
Rate, calculates corrected mortality.By concentration conversion into natural logrithm value, corrected mortality is converted into arcsine transformation value, then with anti-
Sinusoidal replacement value is dependent variable, with dosage natural logrithm value as independent variable, carries out linear regression point with DPS Biological Statistic Analysis Softwares
Analysis, draws the toxicity regression linear equation of each medicament.Correction is obtained according to the toxicity regression linear equation of each medicament dead
Rate arcsine transformation value (y), draws EC by looking into antilogarithm table50Value, then according to the abundant methods of Sun Yun calculate medicament toxicity index and
Co-toxicity coefficient (CTC).
Actual measurement toxicity index=standard agent EC50/ reagent agent EC50×100
Single dose theoretical toxicity index=single dose actual measurement content of the toxicity index × single dose in mixture
Mixture theoretical toxicity index=each single dose theoretical toxicity index sum
Toxicity index/mixture theoretical toxicity index × 100 of co-toxicity coefficient=mixture actual measurement
Evaluation criterion：CTC≤80 are antagonism, and 80 ＜ CTC ＜ 120 are summation action, and CTC >=120 are synergistic effect.
Experimental result such as table 14：
Table 14：The toxicity index of each medicament and Prochloraz dichloro dislike the co-toxicity coefficient of bacterium azoles
| Reagent agent | Proportioning | EC50(mg/kg) | Co-toxicity coefficient |
| Dichloro dislikes bacterium azoles | -- | 7.68 | - |
| Prochloraz | -- | 5.33 | - |
| Dichloro dislikes bacterium azoles：Prochloraz | 1：50 | 3.88 | 138.2 |
| Dichloro dislikes bacterium azoles：Prochloraz | 1：40 | 3.75 | 143.2 |
| Dichloro dislikes bacterium azoles：Prochloraz | 1：30 | 3.21 | 167.7 |
| Dichloro dislikes bacterium azoles：Prochloraz | 1：15 | 2.87 | 189.34 |
| Dichloro dislikes bacterium azoles：Prochloraz | 1：1 | 3.21 | 196.04 |
| Dichloro dislikes bacterium azoles：Prochloraz | 5：1 | 4.01 | 178.41 |
| Dichloro dislikes bacterium azoles：Prochloraz | 10：1 | 4.53 | 163 |
| Dichloro dislikes bacterium azoles：Prochloraz | 15：1 | 4.72 | 158.35 |
| Dichloro dislikes bacterium azoles：Prochloraz | 20：1 | 4.89 | 153.83 |
| Dichloro dislikes bacterium azoles：Prochloraz | 30：1 | 5.12 | 147.9 |
| Dichloro dislikes bacterium azoles：Prochloraz | 40：1 | 5.23 | 145.28 |
| Dichloro dislikes bacterium azoles：Prochloraz | 50：1 | 5.42 | 140.48 |
Table 14 test result indicate that dichloro dislikes bacterium azoles and Prochloraz in mass ratio 1:50～50:1 compounding, to the red spider of oranges and tangerines
Spider has synergistic effect, and especially proportioning is 1:40～30:When between 1, synergy is more notable.
Toxicity Determination of the composition of the present invention to oranges and tangerines resin disease (broken skin disease)：
Test adopts mycelial growth rate method, takes the experiment liquid that 5ml prepares and is added to equipped with the hot culture medium (PDA of 45ml
Culture medium, 45-50 DEG C) conical flask in, after shaking up, pour rapidly diameter 90mm glass culture dish into, each culture dish pours band into
Medicine culture medium 12ml, each process 4 repetitions, horizontal rest, after cooling flat board.With diameter 5mm card punch from culture 4d
Cut bacteria cake for examination bacterium edge, the one side with mycelia is connected on the malicious culture medium of band with pin is chosen, all operations are ultra-clean
Workbench carries out sterile working.Right-angled intersection is adopted after cultivating 4d during flat board to be placed on after process 20 DEG C of constant temperature sterile culture case
Method measures the colony diameter of each process respectively, calculates each mean value for processing colony diameter, the average net growth of colony diameter
And mycelial growth inhibition rate.
Bacterium colony net growth (mm)=colony diameter -5
Mycelial growth inhibition rate (%)=((control bacterium colony net growth-process bacterium colony net growth)/control bacterium colony is net
Increment) × 100 by concentration conversion into natural logrithm value, suppress rate conversion arcsin function value, with arcsine letter replacement value be
Dependent variable, with dosage natural logrithm value as independent variable, carries out linear regression analysis with DPS Biological Statistic Analysis Softwares, draws recurrence side
Journey, then carry out the conversion of anti-natural logrithm and draw EC50.Toxicity index and the co-toxicity coefficient (CTC) of medicament are calculated according to the abundant methods of Sun Yun.
Actual measurement toxicity index=standard agent EC50/ reagent agent EC50×100
Single dose theoretical toxicity index=single dose actual measurement content of the toxicity index × single dose in mixture
Mixture theoretical toxicity index=each single dose theoretical toxicity index sum
Toxicity index/mixture theoretical toxicity index × 100 of co-toxicity coefficient=mixture actual measurement
Evaluation criterion：CTC≤80 are antagonism, and 80 ＜ CTC ＜ 120 are summation action, and CTC >=120 are synergistic effect.
Experimental result such as table 15；
Table 15：The toxicity index of each medicament and Prochloraz dichloro dislike the co-toxicity coefficient of bacterium azoles
| Reagent agent | Proportioning | EC50(mg/kg) | Co-toxicity coefficient |
| Dichloro dislikes bacterium azoles | - | 10.42 | - |
| Prochloraz | - | 6.61 | - |
| Dichloro dislikes bacterium azoles:Prochloraz | 1：50 | 4.71 | 141.35 |
| Dichloro dislikes bacterium azoles:Prochloraz | 1：40 | 4.33 | 154.03 |
| Dichloro dislikes bacterium azoles:Prochloraz | 1：30 | 3.79 | 176.49 |
| Dichloro dislikes bacterium azoles:Prochloraz | 1：15 | 3.42 | 197.80 |
| Dichloro dislikes bacterium azoles:Prochloraz | 1：1 | 3.65 | 221.61 |
| Dichloro dislikes bacterium azoles:Prochloraz | 5：1 | 4.35 | 218.55 |
| Dichloro dislikes bacterium azoles:Prochloraz | 10：1 | 4.88 | 202.89 |
| Dichloro dislikes bacterium azoles:Prochloraz | 15：1 | 5.32 | 189.05 |
| Dichloro dislikes bacterium azoles:Prochloraz | 20：1 | 5.88 | 172.48 |
| Dichloro dislikes bacterium azoles:Prochloraz | 30：1 | 6.45 | 158.60 |
| Dichloro dislikes bacterium azoles:Prochloraz | 40：1 | 6.63 | 154.99 |
| Dichloro dislikes bacterium azoles:Prochloraz | 50：1 | 6.96 | 147.64 |
Table 15 test result indicate that dichloro dislikes bacterium azoles and Prochloraz composition in mass ratio 1:50～50:1 compounding, to mandarin orange
Tangerine fat disease (broken skin disease) has synergistic effect, and especially proportioning is 1：30～20：When between 1, synergy is more notable.The present invention's
Toxicity Determination of the composition to citrus anthracnose：
Test adopts mycelial growth rate method, takes the experiment liquid that 5ml prepares and is added to equipped with the hot culture medium (PDA of 45ml
Culture medium, 45-50 DEG C) conical flask in, after shaking up, pour rapidly diameter 90mm glass culture dish into, each culture dish pours band into
Medicine culture medium 12ml, each process 4 repetitions, horizontal rest, after cooling flat board.With diameter 5mm card punch from culture 4d
Cut bacteria cake for examination bacterium edge, the one side with mycelia is connected on the malicious culture medium of band with pin is chosen, all operations are ultra-clean
Workbench carries out sterile working.Right-angled intersection is adopted after cultivating 4d during flat board to be placed on after process 20 DEG C of constant temperature sterile culture case
Method measures the colony diameter of each process respectively, calculates each mean value for processing colony diameter, the average net growth of colony diameter
And mycelial growth inhibition rate.
Bacterium colony net growth (mm)=colony diameter -5
Mycelial growth inhibition rate (%)=((control bacterium colony net growth-process bacterium colony net growth)/control bacterium colony is net
Increment) × 100 by concentration conversion into natural logrithm value, suppress rate conversion arcsin function value, with arcsine letter replacement value be
Dependent variable, with dosage natural logrithm value as independent variable, carries out linear regression analysis with DPS Biological Statistic Analysis Softwares, draws recurrence side
Journey, then carry out the conversion of anti-natural logrithm and draw EC50.Toxicity index and the co-toxicity coefficient (CTC) of medicament are calculated according to the abundant methods of Sun Yun.
Actual measurement toxicity index=standard agent EC50/ reagent agent EC50×100
Single dose theoretical toxicity index=single dose actual measurement content of the toxicity index × single dose in mixture
Mixture theoretical toxicity index=each single dose theoretical toxicity index sum
Toxicity index/mixture theoretical toxicity index × 100 of co-toxicity coefficient=mixture actual measurement
Evaluation criterion：CTC≤80 are antagonism, and 80 ＜ CTC ＜ 120 are summation action, and CTC >=120 are synergistic effect.
Experimental result such as table 16；
Table 16：The toxicity index of each medicament and Prochloraz dichloro dislike the co-toxicity coefficient of bacterium azoles
Table 16 test result indicate that dichloro dislikes bacterium azoles and Prochloraz composition in mass ratio 1:50～50:1 compounding, to mandarin orange
Tangerine anthracnose has synergistic effect, and especially proportioning is 1：30～40：When between 1, synergy is more notable.
Toxicity Determination of the composition of the present invention to citrus bacterial canker disease：
Test adopts mycelial growth rate method, takes the experiment liquid that 5ml prepares and is added to equipped with the hot culture medium (PDA of 45ml
Culture medium, 45-50 DEG C) conical flask in, after shaking up, pour rapidly diameter 90mm glass culture dish into, each culture dish pours band into
Medicine culture medium 12ml, each process 4 repetitions, horizontal rest, after cooling flat board.With diameter 5mm card punch from culture 4d
Cut bacteria cake for examination bacterium edge, the one side with mycelia is connected on the malicious culture medium of band with pin is chosen, all operations are ultra-clean
Workbench carries out sterile working.Right-angled intersection is adopted after cultivating 4d during flat board to be placed on after process 20 DEG C of constant temperature sterile culture case
Method measures the colony diameter of each process respectively, calculates each mean value for processing colony diameter, the average net growth of colony diameter
And mycelial growth inhibition rate.
Bacterium colony net growth (mm)=colony diameter -5
Mycelial growth inhibition rate (%)=((control bacterium colony net growth-process bacterium colony net growth)/control bacterium colony is net
Increment) × 100 by concentration conversion into natural logrithm value, suppress rate conversion arcsin function value, with arcsine letter replacement value be
Dependent variable, with dosage natural logrithm value as independent variable, carries out linear regression analysis with DPS Biological Statistic Analysis Softwares, draws recurrence side
Journey, then carry out the conversion of anti-natural logrithm and draw EC50.Toxicity index and the co-toxicity coefficient (CTC) of medicament are calculated according to the abundant methods of Sun Yun.
Actual measurement toxicity index=standard agent EC50/ reagent agent EC50×100
Single dose theoretical toxicity index=single dose actual measurement content of the toxicity index × single dose in mixture
Mixture theoretical toxicity index=each single dose theoretical toxicity index sum
Toxicity index/mixture theoretical toxicity index × 100 of co-toxicity coefficient=mixture actual measurement
Evaluation criterion：CTC≤80 are antagonism, and 80 ＜ CTC ＜ 120 are summation action, and CTC >=120 are synergistic effect.
Experimental result such as table 17；
Table 17：The toxicity index of each medicament and Prochloraz dichloro dislike the co-toxicity coefficient of bacterium azoles
Table 17 test result indicate that dichloro dislikes bacterium azoles and Prochloraz composition in mass ratio 1:50～50:1 compounding, to mandarin orange
Tangerine canker has synergistic effect, and especially proportioning is 1：30～30：When between 1, synergy is more notable.
Toxicity Determination of the composition of the present invention to oranges and tangerines downy mildew：
Test adopts mycelial growth rate method, takes the experiment liquid that 5ml prepares and is added to equipped with the hot culture medium (PDA of 45ml
Culture medium, 45-50 DEG C) conical flask in, after shaking up, pour rapidly diameter 90mm glass culture dish into, each culture dish pours band into
Medicine culture medium 12ml, each process 4 repetitions, horizontal rest, after cooling flat board.With diameter 5mm card punch from culture 4d
Cut bacteria cake for examination bacterium edge, the one side with mycelia is connected on the malicious culture medium of band with pin is chosen, all operations are ultra-clean
Workbench carries out sterile working.Right-angled intersection is adopted after cultivating 4d during flat board to be placed on after process 20 DEG C of constant temperature sterile culture case
Method measures the colony diameter of each process respectively, calculates each mean value for processing colony diameter, the average net growth of colony diameter
And mycelial growth inhibition rate.
Bacterium colony net growth (mm)=colony diameter -5
Mycelial growth inhibition rate (%)=((control bacterium colony net growth-process bacterium colony net growth)/control bacterium colony is net
Increment) × 100 by concentration conversion into natural logrithm value, suppress rate conversion arcsin function value, with arcsine letter replacement value be
Dependent variable, with dosage natural logrithm value as independent variable, carries out linear regression analysis with DPS Biological Statistic Analysis Softwares, draws recurrence side
Journey, then carry out the conversion of anti-natural logrithm and draw EC50.Toxicity index and the co-toxicity coefficient (CTC) of medicament are calculated according to the abundant methods of Sun Yun.
Actual measurement toxicity index=standard agent EC50/ reagent agent EC50×100
Single dose theoretical toxicity index=single dose actual measurement content of the toxicity index × single dose in mixture
Mixture theoretical toxicity index=each single dose theoretical toxicity index sum
Toxicity index/mixture theoretical toxicity index × 100 of co-toxicity coefficient=mixture actual measurement
Evaluation criterion：CTC≤80 are antagonism, and 80 ＜ CTC ＜ 120 are summation action, and CTC >=120 are synergistic effect.
Experimental result such as table 18；
Table 18：The toxicity index of each medicament and Prochloraz dichloro dislike the co-toxicity coefficient of bacterium azoles
| Reagent agent | Proportioning | EC50(mg/kg) | Co-toxicity coefficient |
| Dichloro dislikes bacterium azoles | - | 8.46 | - |
| Prochloraz | - | 6.59 | - |
| Dichloro dislikes bacterium azoles:Prochloraz | 1:50 | 4.88 | 135.63 |
| Dichloro dislikes bacterium azoles:Prochloraz | 1:40 | 4.52 | 146.59 |
| Dichloro dislikes bacterium azoles:Prochloraz | 1:30 | 4.11 | 161.49 |
| Dichloro dislikes bacterium azoles:Prochloraz | 1:20 | 3.89 | 171.21 |
| Dichloro dislikes bacterium azoles:Prochloraz | 1:15 | 3.51 | 190.38 |
| Dichloro dislikes bacterium azoles:Prochloraz | 1:10 | 3.28 | 205.03 |
| Dichloro dislikes bacterium azoles:Prochloraz | 1:1 | 3.49 | 212.29 |
| Dichloro dislikes bacterium azoles:Prochloraz | 5:1 | 3.89 | 207.66 |
| Dichloro dislikes bacterium azoles:Prochloraz | 10:1 | 4.28 | 192.69 |
| Dichloro dislikes bacterium azoles:Prochloraz | 15:1 | 4.69 | 177.24 |
| Dichloro dislikes bacterium azoles:Prochloraz | 20:1 | 4.93 | 169.31 |
| Dichloro dislikes bacterium azoles:Prochloraz | 30:1 | 5.24 | 159.99 |
| Dichloro dislikes bacterium azoles:Prochloraz | 40:1 | 5.51 | 152.48 |
| Dichloro dislikes bacterium azoles:Prochloraz | 50:1 | 5.96 | 141.16 |
Table 18 test result indicate that dichloro dislikes bacterium azoles and Prochloraz composition in mass ratio 1:50～50:1 compounding, to mandarin orange
Tangerine downy mildew has synergistic effect, and especially proportioning is 1：30～30：When between 1, synergy is more notable.
Toxicity Determination of the composition of the present invention to oranges and tangerines gray mold：
Test adopts mycelial growth rate method, takes the experiment liquid that 5ml prepares and is added to equipped with the hot culture medium (PDA of 45ml
Culture medium, 45-50 DEG C) conical flask in, after shaking up, pour rapidly diameter 90mm glass culture dish into, each culture dish pours band into
Medicine culture medium 12ml, each process 4 repetitions, horizontal rest, after cooling flat board.With diameter 5mm card punch from culture 4d
Cut bacteria cake for examination bacterium edge, the one side with mycelia is connected on the malicious culture medium of band with pin is chosen, all operations are ultra-clean
Workbench carries out sterile working.Right-angled intersection is adopted after cultivating 4d during flat board to be placed on after process 20 DEG C of constant temperature sterile culture case
Method measures the colony diameter of each process respectively, calculates each mean value for processing colony diameter, the average net growth of colony diameter
And mycelial growth inhibition rate.
Bacterium colony net growth (mm)=colony diameter -5
Mycelial growth inhibition rate (%)=((control bacterium colony net growth-process bacterium colony net growth)/control bacterium colony is net
Increment) × 100 by concentration conversion into natural logrithm value, suppress rate conversion arcsin function value, with arcsine letter replacement value be
Dependent variable, with dosage natural logrithm value as independent variable, carries out linear regression analysis with DPS Biological Statistic Analysis Softwares, draws recurrence side
Journey, then carry out the conversion of anti-natural logrithm and draw EC50.Toxicity index and the co-toxicity coefficient (CTC) of medicament are calculated according to the abundant methods of Sun Yun.
Actual measurement toxicity index=standard agent EC50/ reagent agent EC50×100
Single dose theoretical toxicity index=single dose actual measurement content of the toxicity index × single dose in mixture
Mixture theoretical toxicity index=each single dose theoretical toxicity index sum
Toxicity index/mixture theoretical toxicity index × 100 of co-toxicity coefficient=mixture actual measurement
Evaluation criterion：CTC≤80 are antagonism, and 80 ＜ CTC ＜ 120 are summation action, and CTC >=120 are synergistic effect.
Experimental result such as table 19；
Table 19：The toxicity index of each medicament and Prochloraz dichloro dislike the co-toxicity coefficient of bacterium azoles
| Reagent agent | Proportioning | EC50(mg/kg) | Co-toxicity coefficient |
| Dichloro dislikes bacterium azoles | - | 8.25 | - |
| Prochloraz | - | 6.16 | - |
| Dichloro dislikes bacterium azoles:Prochloraz | 1:50 | 4.68 | 132.28 |
| Dichloro dislikes bacterium azoles:Prochloraz | 1:40 | 4.21 | 147.23 |
| Dichloro dislikes bacterium azoles:Prochloraz | 1:30 | 3.98 | 156.05 |
| Dichloro dislikes bacterium azoles:Prochloraz | 1:20 | 3.61 | 172.72 |
| Dichloro dislikes bacterium azoles:Prochloraz | 1:15 | 3.41 | 183.55 |
| Dichloro dislikes bacterium azoles:Prochloraz | 1:10 | 3.16 | 199.53 |
| Dichloro dislikes bacterium azoles:Prochloraz | 1:1 | 3.34 | 211.18 |
| Dichloro dislikes bacterium azoles:Prochloraz | 5:1 | 3.85 | 202.82 |
| Dichloro dislikes bacterium azoles:Prochloraz | 10:1 | 4.11 | 194.72 |
| Dichloro dislikes bacterium azoles:Prochloraz | 15:1 | 4.42 | 182.78 |
| Dichloro dislikes bacterium azoles:Prochloraz | 20:1 | 4.69 | 173.11 |
| Dichloro dislikes bacterium azoles:Prochloraz | 30:1 | 4.99 | 163.54 |
| Dichloro dislikes bacterium azoles:Prochloraz | 40:1 | 5.26 | 155.56 |
| Dichloro dislikes bacterium azoles:Prochloraz | 50:1 | 5.79 | 141.32 |
Table 19 test result indicate that dichloro dislikes bacterium azoles and Prochloraz composition in mass ratio 1:50～50:1 compounding, to mandarin orange
Tangerine gray mold has synergistic effect, and especially proportioning is 1：30～40：When between 1, synergy is more notable.
Toxicity Determination of the composition of the present invention to oranges and tangerines blister mite：
The slide infusion process that test is recommended using FAO (Food and Agriculture Organization of the United Nation) (FAO).Every piece of slide be inoculated with 20 it is living
It is female to set series concentration value on the basis of 3 pilot studies into mite, clear water control, often process 3 repetitions (totally 60 it is female into
Mite).Leaching medicine is blotted with blotting paper after 5 seconds immediately, is then placed in 24-26 DEG C of insulating box, checks dead within 48 hours after medicine
Rate, calculates corrected mortality.By concentration conversion into natural logrithm value, corrected mortality is converted into arcsine transformation value, then with anti-
Sinusoidal replacement value is dependent variable, with dosage natural logrithm value as independent variable, carries out linear regression point with DPS Biological Statistic Analysis Softwares
Analysis, draws the toxicity regression linear equation of each medicament.Correction is obtained according to the toxicity regression linear equation of each medicament dead
Rate arcsine transformation value (y), draws EC by looking into antilogarithm table50Value, then according to the abundant methods of Sun Yun calculate medicament toxicity index and
Co-toxicity coefficient (CTC).
Actual measurement toxicity index=standard agent EC50/ reagent agent EC50×100
Single dose theoretical toxicity index=single dose actual measurement content of the toxicity index × single dose in mixture
Mixture theoretical toxicity index=each single dose theoretical toxicity index sum
Toxicity index/mixture theoretical toxicity index × 100 of co-toxicity coefficient=mixture actual measurement
Evaluation criterion：CTC≤80 are antagonism, and 80 ＜ CTC ＜ 120 are summation action, and CTC >=120 are synergistic effect.
Experimental result such as table 20
Table 20：The toxicity index of each medicament and Prochloraz dichloro dislike the co-toxicity coefficient of bacterium azoles
| Reagent agent | Proportioning | EC50(mg/kg) | Co-toxicity coefficient |
| Dichloro dislikes bacterium azoles | -- | 7.65 | - |
| Prochloraz | -- | 5.31 | - |
| Dichloro dislikes bacterium azoles：Prochloraz | 1:50 | 4.12 | 129.66 |
| Dichloro dislikes bacterium azoles：Prochloraz | 1:40 | 3.64 | 146.98 |
| Dichloro dislikes bacterium azoles：Prochloraz | 1:30 | 3.31 | 162.02 |
| Dichloro dislikes bacterium azoles：Prochloraz | 1:20 | 2.98 | 181.66 |
| Dichloro dislikes bacterium azoles：Prochloraz | 1:15 | 2.74 | 197.57 |
| Dichloro dislikes bacterium azoles：Prochloraz | 1:10 | 2.58 | 211.7 |
| Dichloro dislikes bacterium azoles：Prochloraz | 1:1 | 2.82 | 222.3 |
| Dichloro dislikes bacterium azoles：Prochloraz | 5:1 | 3.11 | 229.15 |
| Dichloro dislikes bacterium azoles：Prochloraz | 10:1 | 3.54 | 207.78 |
| Dichloro dislikes bacterium azoles：Prochloraz | 15:1 | 3.79 | 196.44 |
| Dichloro dislikes bacterium azoles：Prochloraz | 20:1 | 4.01 | 186.85 |
| Dichloro dislikes bacterium azoles：Prochloraz | 30:1 | 4.46 | 169.5 |
Table 20 test result indicate that dichloro dislikes bacterium azoles and Prochloraz in mass ratio 1:50～50:1 compounding, become rusty wall to oranges and tangerines
Lice has synergistic effect, and especially proportioning is 1:20～20:When between 1, synergy is more notable.
Toxicity Determination of the composition of the present invention to orange caterpillar：
The slide infusion process that test is recommended using FAO (Food and Agriculture Organization of the United Nation) (FAO).Every piece of slide be inoculated with 20 it is living
It is female to set series concentration value on the basis of 3 pilot studies into mite, clear water control, often process 3 repetitions (totally 60 it is female into
Mite).Leaching medicine is blotted with blotting paper after 5 seconds immediately, is then placed in 24-26 DEG C of insulating box, checks dead within 48 hours after medicine
Rate, calculates corrected mortality.By concentration conversion into natural logrithm value, corrected mortality is converted into arcsine transformation value, then with anti-
Sinusoidal replacement value is dependent variable, with dosage natural logrithm value as independent variable, carries out linear regression point with DPS Biological Statistic Analysis Softwares
Analysis, draws the toxicity regression linear equation of each medicament.Correction is obtained according to the toxicity regression linear equation of each medicament dead
Rate arcsine transformation value (y), draws EC by looking into antilogarithm table50Value, then according to the abundant methods of Sun Yun calculate medicament toxicity index and
Co-toxicity coefficient (CTC).
Actual measurement toxicity index=standard agent EC50/ reagent agent EC50×100
Single dose theoretical toxicity index=single dose actual measurement content of the toxicity index × single dose in mixture
Mixture theoretical toxicity index=each single dose theoretical toxicity index sum
Toxicity index/mixture theoretical toxicity index × 100 of co-toxicity coefficient=mixture actual measurement
Evaluation criterion：CTC≤80 are antagonism, and 80 ＜ CTC ＜ 120 are summation action, and CTC >=120 are synergistic effect.
Experimental result such as table 21：
Table 21：The toxicity index of each medicament and Prochloraz dichloro dislike the co-toxicity coefficient of bacterium azoles
Table 21 test result indicate that dichloro dislikes bacterium azoles and Prochloraz in mass ratio 1:50～50:1 compounding, to orange caterpillar
There is synergistic effect, especially proportioning is 1:40～30:When between 1, synergy is more notable.
Claims (10)
1. a kind of composition pesticide for disliking bacterium azoles and Prochloraz containing dichloro, including active component, it is characterised in that：It is described activity into
It is divided into dichloro and dislikes bacterium azoles and Prochloraz, is calculated by weight as：
Dichloro dislikes bacterium azoles 1-50 parts；
Prochloraz 1-50 parts.
2. a kind of composition pesticide for disliking bacterium azoles and Prochloraz containing dichloro according to claim 1, it is characterised in that：It is described
It is 1-5 parts that dichloro dislikes bacterium azoles, and the Prochloraz is 1-50 parts.
3. a kind of composition pesticide for disliking bacterium azoles and Prochloraz containing dichloro according to claim 1, it is characterised in that：It is described
It is 1-10 parts that dichloro dislikes bacterium azoles, and the Prochloraz is 1-30 parts.
4. a kind of composition pesticide for disliking bacterium azoles and Prochloraz containing dichloro according to claim 1, it is characterised in that：It is described
It is 1-20 parts that dichloro dislikes bacterium azoles, and the Prochloraz is 1-30 parts.
5. a kind of composition pesticide for disliking bacterium azoles and Prochloraz containing dichloro according to claim 1, it is characterised in that：It is described
It is 1-30 parts that dichloro dislikes bacterium azoles, and the Prochloraz is 1-30 parts.
6. a kind of composition pesticide for disliking bacterium azoles and Prochloraz containing dichloro according to claim 1, it is characterised in that：It is described
It is 1-30 parts that dichloro dislikes bacterium azoles, and the Prochloraz is 1-20 parts.
7. a kind of composition pesticide for disliking bacterium azoles and Prochloraz containing dichloro according to any one of claim 1-6, which is special
Levy and be：The composition pesticide also include auxiliary agent, the auxiliary agent include dispersant, emulsifying agent, antifreezing agent, thickener, defoamer,
One or more in solvent, wetting agent, disintegrant and filler.
8. a kind of composition pesticide for disliking bacterium azoles and Prochloraz containing dichloro according to claim 7, it is characterised in that：It is described
Auxiliary agent addition parts by weight be：
Dispersant 0.1-15 parts；
Emulsifying agent 0.1-12 parts；
Antifreezing agent 0.1-4 parts；
Thickener 0.1-4 parts；
Defoamer 0.1-2 parts；
Solvent 25.9-95.8 parts；
Wetting agent 0.1-15 parts；
Disintegrant 0.1-6 parts；
Filler 8-97.5 parts.
9. a kind of composition pesticide for disliking bacterium azoles and Prochloraz containing dichloro according to claim 7, it is characterised in that：It is described
Emulsifying agent is calcium dodecyl benzene sulfonate, alkylpolyoxyethylene, styrylphenol polyoxyethylene ether, polyoxyethylene nonyl phenyl
Vinethene, thermally coupled distillation columns, aliphatic acid polyethenoxy base ester, polyoxyethylene aliphatic alcohol ether, dodecyl gather
One or more combination in oxygen ethenesulfonic acid sodium salt and phenethyl phenol polyethenoxy ether；
The solvent is dimethylbenzene, diesel oil, toluene, cyclohexanol, methyl alcohol, soybean oil, turpentine oil, methyl oleate, solvent naphtha, diformazan
One or more combination in base formamide, ethanol, N- pyrrolidones, ethyl acetate and water；
The wetting agent is lauryl sodium sulfate, calcium dodecyl benzene sulfonate, methyl naphthalene sulfonate formaldehyde condensate, pulls open
One or more combination in powder, Negel and spaonin powder；
The dispersant be sodium lignin sulfonate, calcium lignosulfonate, APES formaldehyde condensation product sodium sulphate, ten
Dialkyl benzene sulfonic acids calcium, APES, polycarboxylic acids sodium, aliphatic amine polyoxyethylene ether and glycerin polyoxyethylene ether fat
One or more combination in acid esters；
The antifreezing agent is one or more the combination in ethylene glycol, propane diols, glycerine and urea；
The thickener is one or more the group in xanthans, Magnesiumaluminumsilicate, hydroxymethyl cellulose and polyvinyl alcohol
Close；
The defoamer is one or more the combination in silicone oil, organic silicone and polypropylene glycerol aether；
The disintegrant is one or more the group in bentonite, ammonium sulfate, sodium acid carbonate, aluminium chloride and succinic acid
Close；
Described filler is one or more the combination in white carbon, kaolin, diatomite, precipitated calcium carbonate and starch.
10. a kind of composition pesticide for disliking bacterium azoles and Prochloraz containing dichloro according to claim 7, it is characterised in that：Agriculture
The formulation of drug composition is suspending agent, aqueous emulsion, water dispersible granules, microemulsion or wettable powder.
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| CN201610791092.9A CN106508944A (en) | 2016-08-31 | 2016-08-31 | Pesticidal composition containing 2-methylsulfonyl-5-(2,4-dichlorophenyl)-1,3,4-oxadiazole and prochloraz |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610791092.9A CN106508944A (en) | 2016-08-31 | 2016-08-31 | Pesticidal composition containing 2-methylsulfonyl-5-(2,4-dichlorophenyl)-1,3,4-oxadiazole and prochloraz |
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Family
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|---|---|---|---|
| CN201610791092.9A Pending CN106508944A (en) | 2016-08-31 | 2016-08-31 | Pesticidal composition containing 2-methylsulfonyl-5-(2,4-dichlorophenyl)-1,3,4-oxadiazole and prochloraz |
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| CN109832271A (en) * | 2017-11-27 | 2019-06-04 | 佛山市盈辉作物科学有限公司 | A kind of bactericidal composition for disliking bacterium azoles containing bromothalonil and dichloro |
| CN110376341A (en) * | 2019-06-24 | 2019-10-25 | 广西大学 | A kind of measuring method of graw mold of tomato protective agents photolysis |
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| CN101796955A (en) * | 2009-12-31 | 2010-08-11 | 青岛星牌作物科学有限公司 | Composite bactericidal composite and applications thereof |
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| CN101796955A (en) * | 2009-12-31 | 2010-08-11 | 青岛星牌作物科学有限公司 | Composite bactericidal composite and applications thereof |
| CN104472478A (en) * | 2014-12-13 | 2015-04-01 | 贵州大学 | Suspending agent for C9H6Cl2N2O3S |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109832271A (en) * | 2017-11-27 | 2019-06-04 | 佛山市盈辉作物科学有限公司 | A kind of bactericidal composition for disliking bacterium azoles containing bromothalonil and dichloro |
| CN110376341A (en) * | 2019-06-24 | 2019-10-25 | 广西大学 | A kind of measuring method of graw mold of tomato protective agents photolysis |
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