CN108184520A - A kind of ecological agriculture leisure picking management mode - Google Patents

A kind of ecological agriculture leisure picking management mode Download PDF

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Publication number
CN108184520A
CN108184520A CN201711486326.XA CN201711486326A CN108184520A CN 108184520 A CN108184520 A CN 108184520A CN 201711486326 A CN201711486326 A CN 201711486326A CN 108184520 A CN108184520 A CN 108184520A
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garden
plantation
aquaculture
temperature
starfish
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Chinese (zh)
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曲翠平
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Individual
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005—Cultivation methods
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C21/00—Methods of fertilising, sowing or planting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00—Protecting plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00—Culture of aquatic animals
    • A01K61/10—Culture of aquatic animals of fish
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00—Culture of aquatic animals
    • A01K61/50—Culture of aquatic animals of shellfish
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00—Culture of aquatic animals
    • A01K61/50—Culture of aquatic animals of shellfish
    • A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/02—Breeding vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/06—Aluminium; Calcium; Magnesium; Compounds thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/16—Heavy metals; Compounds thereof
    • A01N59/20—Copper
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
    • A01N61/02—Mineral oils; Tar oils; Tar; Distillates, extracts or conversion products thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80—Soil conditioners
    • E—FIXED CONSTRUCTIONS
    • E04—BUILDING
    • E04H—BUILDINGS OR LIKE STRUCTURES FOR PARTICULAR PURPOSES; SWIMMING OR SPLASH BATHS OR POOLS; MASTS; FENCING; TENTS OR CANOPIES, IN GENERAL
    • E04H5/00—Buildings or groups of buildings for industrial or agricultural purposes
    • E04H5/08—Buildings or groups of buildings for agricultural purposes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/28—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture specially adapted for farming
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/60—Fishing; Aquaculture; Aquafarming
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Plant Pathology (AREA)
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Abstract

It lies fallow the present invention relates to a kind of ecological agriculture and picks management mode, villa garden circulation pattern is cultivated including characteristic kind, characteristic kind cultivates villa garden circulation pattern and is made of plantation, beast fowl cultivation garden, aquaculture garden, food and drink garden, amusement and recreation garden and product processing garden, integrate sightseeing, picking, tourism, food and drink and amusement and recreation, quality services are provided for tourist.

Description

A kind of ecological agriculture leisure picking management mode
Technical field
It lies fallow the present invention relates to a kind of ecological agriculture and picks management mode.
Background technology
The ecological agriculture is the staple of conversation that Chinese society advocates now, how to realize circular economy, to greatest extent Optimize the utilization of resources, be the important development direction to develop eco-agriculture.
With social progress and improvement of living standard, people are food-safe and amusement and recreation require higher and higher, mesh It is preceding that there has been no the management modes for meeting people's demand.
Invention content
It lies fallow the technical problem to be solved by the invention is to provide a kind of ecological agriculture and picks management mode, the present invention is logical Plant of the interplanting with expelling parasite insecticidal effect in orchard is crossed, can achieve the purpose that expelling parasite and reduce Pesticide use amount.
In order to solve the above-mentioned technical problem, the present invention uses following technical scheme：
A kind of ecological agriculture leisure picking management mode, which is characterized in that cultivate villa garden circulation pattern including characteristic kind；
The characteristic kind cultivation villa garden circulation pattern includes plantation, beast fowl cultivation garden, aquaculture garden, food and drink garden, stops One in not busy amusement garden and product processing garden or arbitrarily combine two or more；
The plantation is used to plant economic plants；
The beast fowl cultivation garden is used to cultivate beast fowl；
The aquaculture garden is used for aquatic kind；
The food and drink garden is used for one or two in providing food and drink for tourist and stay；
The amusement and recreation garden is used to provide in aquatic recreational amenities, ground recreation and aerial recreation for tourist One or arbitrarily combine two or more；
The product processing garden is used for the economic plants in the plantation, the beast fowl kind in beast fowl cultivation garden, water Aquatic products in production cultivation garden are processed sale and the brewing of drinks；
After straw and fruit that the plantation generates are processed available for the brutish kind in beast fowl cultivation garden and Aquatic products in aquaculture garden；The economic plant that the metabolic waste of brutish kind can be used in breeding and planting garden in beast fowl cultivation garden Aquatic products in object and aquaculture garden；The mud of aquaculture garden bottom of pond can be used in plantation as nutriment, leisure joy It can be used for botanical garden and beast fowl cultivation garden after the filtered disinfection of the water in paradise and aquaculture garden.
The plantation is irrigated using trickle irrigation；Fertilizer uses self-prepared organic fertilizer or biological organic fertilizer；Pollination is using hornet With one or both of spontaneous pollination combination；Insect prevention is using plant of the plantation with anthelminthic effect and puts beneficial insect and mite in a suitable place to breed The combination of one or both of worm；Diseases prevention is using self-control biological pesticide, sulphur mixture, Bordeaux mixture, mineral oil and trichoderma harzianum In one kind or arbitrarily combine two or more；The plant of the anthelminthic effect is garlic, lavender, fish pelargonium, common nepenthes, food One kind in cordyceps sinensis and folium artemisiae argyi arbitrarily combines two or more；The beneficial insect be coccinella septempunctata, the orange spider dragonfly of pyriform, One kind in Chrysopa, ichneumon wasp and trichogramma arbitrarily combines two or more；The beneficial acarid is the grazing eclipse acarid in the Holy Land.
The plantation for plant economic crops including one or both of vegetables, fruit tree, herbage and Chinese medicine with On；
The beast fowl cultivation garden is used to cultivate one or more of chicken, duck, goose, pig, ox and sheep；
The aquaculture garden is for one or more of cultured fishes, crab class, shrimps, shell and Chelonian；
The food and drink garden is used to utilize the industrial crops in plantation, the beast fowl in beast fowl cultivation garden, aquaculture garden In aquatic products and product processing garden processing food and brewing drinks provide food and beverage sevice；
The amusement and recreation garden is for offer swimming, hot spring bathing, trampoline, Wild Mouse, carrousel, roller-coaster and touches One in bumper car or arbitrarily combine two or more；
The product processing garden is used for food processing, brewing, brewed spirit, wine production, dairy produce and fruit juice One or more of manufacture.
Including methane-generating pit, the methane-generating pit is formed methane using the metabolic waste that animal generates and biogas residue, and the biogas is Plantation, beast fowl cultivation garden, aquaculture garden, food and drink garden, amusement and recreation garden and product processing garden provide the energy, and the biogas residue is used Come the industrial crops in breeding and planting garden and the aquatic products in aquaculture garden.
The plantation, beast fowl cultivation garden, aquaculture garden, food and drink garden, amusement and recreation garden and product processing garden at the top of with And several solar panels are installed on campus portion vacant lot, the solar panel is connected with header box, and passes through institute State header box and be uniformly connected to DC cabinet, the DC cabinet connects inverter, the inverter after electric quantity metering device, and Enter national grid.
Self-control biological pesticide is made according to following steps：
By starfish saponin liquid, extracts of Chinese herbal medicine, auxiliary agent and vegetable oil according to mass ratio 20~30:20~30:10~20: 30~50 are added in homogenizer, at least homogeneous once to get biological pesticide；
The starfish saponin liquid follows the steps below：
A) starfish powder is prepared：The starfish after impurity dries will be removed and be crushed to 80~200 mesh, obtain starfish powder；
B) irradiation starfish powder is prepared：Starfish powder is placed in 20~100min of irradiation under far infrared, starfish powder must be irradiated；
C) starfish saponin liquid is prepared:Will irradiation starfish powder and solvent it is miscible after, be added in flash extracter, be in temperature It is extracted under the conditions of 5~30 DEG C, the mass ratio of starfish powder and solvent is 1:9~40, it is filtered after extraction, gained filtrate is sea Star saponin(e liquid；
The extraction voltage of the homogenate extraction be 100~150V, rotating speed be 5000~10000rpm/min, extraction time For 3~20min；
The auxiliary agent is two of the one or any ratio in wetting agent, dispersant, stabilizer, sustained release agent and synergist Kind or more；
The temperature condition of the radiation treatment is 150~250 DEG C；
The solvent is the two or more of the one or any ratio in ethyl alcohol, methanol, methyl acetate and ether；
The vegetable oil is the one or any ratio in palm oil, coconut oil, chinaberry seeds oil, mandarin orange oil and fruit of cubeb litsea tree oil It is two or more；
The extracts of Chinese herbal medicine is trifoliate jewelvine extracting solution and Herba Houttuyniae extract, and extracting method is according to following steps It carries out：
1) not good liquor is prepared：By Chinese herbal medicine and water according to mass ratio 1:5~40 are added in container, are heated to 50~75 DEG C, 20~90min of constant temperature is cooled to 20~40 DEG C, and filtering, gained filtrate is a not good liquor；Residue obtained is the dregs of a decoction；
2) two not good liquors are prepared：By the dregs of a decoction and water according to mass ratio 1:5~40 are added in container, are heated to 70~120 DEG C, Under the conditions of pressure is 0~5MPa, 20~90min of constant temperature is cooled to 20~40 DEG C, and filtering, gained filtrate is two not good liquors；
3) extracting solution is prepared：Two not good liquors obtained by a not good liquor obtained by step 1) and step 2) are merged, are 20~90 in temperature DEG C, under the conditions of pressure is -5MPa~0MPa, 0~90min is concentrated, gained concentrate is extracting solution.
Preferably, homogeneous 2 times, wherein first time homogeneous pressure is 15~35MPa, and homogeneous temperature is 50~70 DEG C, homogeneous Time is 10~60min；Second homogeneous pressure is 2~8PMa, and homogeneous temperature is 50~70 DEG C, the homogeneous time for 10~ 60min。
Preferably, the irradiation temperature described in step b) is 180 DEG C, and exposure time is 40min.
Self-prepared organic fertilizer follows the steps below：
1) fermentate, is prepared：By remaining leftover bits and pieces in the animal wastes in beast fowl cultivation garden, biogas residue, product processing garden processing Material, urea, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose are according to mass ratio 30~99:0~30:0~30:0~4:0~ 1:0~2:0~4:0~4 is added to mixing in the fermentation tank to sterilize, and it is 50~70% to be adjusted to water content with water, must mix training The mass ratio of foster base, access candida utili, candida utili and mixed culture medium is 0.2~2:98~99.8, in temperature It is 25~35 DEG C to spend, and under the conditions of oxygen content is 0.1~0.5mg/L, is fermented 2~7 days, is gone out under the conditions of being 70~150 DEG C in temperature 20~90min of bacterium, obtains fermentate；
2) ammonia-producing bacterium fermentate, is prepared：Corynebacterium ammoniagenes prepared by thawing method, fermentate and thawing are accessed in fermentate The mass ratio of corynebacterium ammoniagenes prepared by method is 95~99:1~5, it is 25~35 DEG C in temperature, water content 50~70% is oxygen-containing It measures under the conditions of 1~5mg/L, to ferment 2~7 days to get production ammoniacal fermentation object；
3) organic fertilizer, is prepared：Ammoniacal fermentation object is produced after being granulated, drying, cool down and sieve to get organic fertilizer.
The preparation of thawing method corynebacterium ammoniagenes follows the steps below：
A) expansion of strain is further cultured for：Using the thalline in exponential phase as seed, it is inoculated with 10% inoculum concentration In fluid nutrient medium, 48h is cultivated in 30 DEG C of shaking table 160r/min, after 8000r/min centrifuges 10min, expansion is obtained and is further cultured for Strain；
B) thawing method improves the permeability of cell：Thawing method is at least repeated once, and step is：The expansion that step 1) is obtained It is further cultured for strain greatly to be put into -20 DEG C of refrigerator, freezes 4h, take out, dissolve at room temperature；
PH7.2~7.4 of the fluid nutrient medium, and be made into according to following mass ratio：Beef infusion broth 1.0L, peptone 10.0g, NaCl 5.0g, agar 25.0g；
The preparation method of wherein beef infusion broth follows the steps below：Lean beef is cleaned, and chopping weighs 550 grams of immersions It in 1375ml water, impregnates a night, filtering, 0.6kg/cm after filtrate packing2Sterilize 40min.
Invention has following advantageous effects：
1st, the present invention integrates sightseeing, picking, tourism, food and drink and amusement and recreation, and quality services are provided for tourist.
2nd, the present invention can be reached self-sufficiency using cyclic culture pattern, non-harmful product is provided for tourist substantially.
3rd, the present invention, can by interplanting the plant with expelling parasite insecticidal effect in orchard or putting beneficial insect and acarid in a suitable place to breed To reach expelling parasite, insecticidal action, Pesticide use amount is reduced.
4th, the present invention uses trifoliate jewelvine extracting solution, contains rotenone in trifoliate jewelvine, and rotenone is very strong to insect and toxicity in fish, and To mammal, then toxicity is very light.Therefore it will not damage to people and animals, not destroy the ecological balance.
5th, extracts of Chinese herbal medicine of the present invention, which is adopted, is extracted with water, and there is no problem of solvent residual, do not generate waste water and exhaust gas, no Environment can be damaged.
6th, the present invention is extracted using secondary water, and low molecular weight can be prevented to be dissolved in the active matter of water, decomposed in the high temperature environment, Ensure the integrality of extract.
7th, the present invention is irradiated using far infrared, can improve the content of starfish saponin.
8th, the present invention carries out extract using homogenizer secondary homogeneous, makes emulsion-stabilizing, easy to use.
9th, the preferred fruit of cubeb litsea tree oil of vegetable oil of the present invention, fruit of cubeb litsea tree oil main component citral (60%~80%), methyl heptan Ketenes (2.5%), citronellal (1.0%), australene, amphene, limonene, α-humulene, p-isopropyl methanol, geraniol, camphor. Camphor and australene have desinsection and anthelmintic action, can significantly increase the drug effect of the present invention.
10th, the present invention is fermented under anaerobic using aerobic and anaerobism candida utili, can prevent nutritional ingredient Loss, and anaerobic fermentation generate alcohol to extraction be a supporting role.
11st, the present invention is fermented under anoxic conditions using aerobic and anaerobism corynebacterium ammoniagenes, produces ATP, and ATP is a kind of High energy phosphate compound, in cell, it can realize energy storage and exoergic with mutually converting for ADP, so as to ensure that cell items The energy supply of vital movement promotes plant growth.It is unreasonable due to long-term fertilization, cause soil acidification, corynebacterium ammoniagenes N0 can be restored3 -And urea, generating ammonia makes tunning that can improve acidified soil in alkalescent.
12nd, the present invention has heavy metal certain fixed function.Contain polypeptide, organic acid and other in fermentate of the present invention Protide, heavy metal can make albumen, organic acid and polypeptide inactivation, while heavy metal also can be by albumen, alginic acid and polypeptide It is fixed, so as to prevent crop from absorbing.
13rd, current soil hardening is serious, applies the microorganism in ground often in anaerobic condition, more can using aerobic and oxygen bacterium Adapt to present soil environment.
14th, the permeability of corynebacterium ammoniagenes cell is improved by thawing method, the ATP that corynebacterium ammoniagenes generate can be made more to hold It is easily penetrated into from cell membrane extracellularly, product effects are significantly better than the product of untreated corynebacterium ammoniagenes.
Specific embodiment
It is further illustrated the present invention with reference to specific example.
Embodiment 1
The characteristic kind cultivation villa garden circulation pattern includes plantation, beast fowl cultivation garden, aquaculture garden, food and drink garden, stops Not busy amusement garden and product processing garden；
Tourist, which enters plantation, can watch and pick the economic plants planted in plantation, and economic plants has rape, white Dish, peach, cherry tree, strawberry, herbage, purslane and Herba Euphorbiae Humifusae；
Tourist, which enters beast fowl cultivation garden, can watch and buy the beast fowl cultivated in beast fowl cultivation garden, beast fowl have chicken, duck, Goose, pig, ox, Ma Heyang；
Tourist, which enters aquaculture garden, can watch the aquatic products cultivated in aquaculture garden of fishing, aquatic products have carp, Grass carp, crab methyl red, prawn, grass shrimp, cray, scallop and tortoise；
The food and drink garden is used to provide food and drink and lodging for tourist；
Tourist can carry out Drifting, hot spring bathing, trampoline, dodgem and Rollercoaster amusement in amusement and recreation garden and live It is dynamic；
The product processing garden is used for food processing, brewing, brewed spirit, wine production, dairy produce and fruit juice Manufacture, tourist can buy according to oneself demand；
The processed rear formation feed of straw and fruit that plantation generates, for the brutish kind in beast fowl cultivation garden And the nursing of the aquatic products in aquaculture garden；Organic fertilizer is formed after the metabolic waste of brutish kind is processed in beast fowl cultivation garden Or biological organic fertilizer or feed, for supplying the nutriment needed for the economic plants in plantation and the aquatic products in aquaculture garden Feed；The mud of aquaculture garden bottom of pond can be used in plantation as nutriment, amusement and recreation garden and aquaculture garden Animal drinks in irrigation and beast fowl cultivation garden after the filtered disinfection of water available for botanical garden.
The plantation is irrigated using trickle irrigation；Fertilizer uses beast poultry manure in beast fowl cultivation garden, biogas residue, product processing garden to add Organic fertilizer or biological organic fertilizer is made in remaining leftover bits and pieces in work；Pollination is using hornet and spontaneous pollination；Insect prevention is using plantation Garlic, lavender, fish pelargonium, common nepenthes and shichongcao and put in a suitable place to breed the orange spider dragonfly of coccinella septempunctata, pyriform, Chrysopa, ichneumon wasp and Trichogramma and the grazing eclipse acarid in the Holy Land；Diseases prevention is using self-control biological pesticide, sulphur mixture, Bordeaux mixture, mineral oil and Ha Ci wood Mould.
Characteristic kind cultivation villa garden circulation pattern further includes methane-generating pit, and the methane-generating pit is given birth to using the metabolic waste that animal generates Into biogas and biogas residue, the biogas is plantation, beast fowl cultivates garden, aquaculture garden, food and drink garden, amusement and recreation garden and product and adds Work garden provides the energy, and the biogas residue is used for the industrial crops in breeding and planting garden and the aquatic products in aquaculture garden.
The plantation, beast fowl cultivation garden, aquaculture garden, food and drink garden, amusement and recreation garden and product processing garden at the top of with And several solar panels are installed on campus portion vacant lot, the solar panel is connected with header box, and passes through institute State header box and be uniformly connected to DC cabinet, the DC cabinet connects inverter, the inverter after electric quantity metering device, and Enter national grid.
Self-control biological pesticide is made according to following steps：
By starfish saponin liquid, extracts of Chinese herbal medicine, auxiliary agent and vegetable oil according to mass ratio 25:25:15:35 be added to it is homogeneous In machine, homogeneous 2 times, wherein first time homogeneous pressure is 30MPa, and homogeneous temperature is 60 DEG C, and the homogeneous time is 20min；Second Homogeneous pressure is 4PMa, and homogeneous temperature is 60 DEG C, and the homogeneous time is 30min to get biological pesticide；
The starfish saponin liquid follows the steps below：
A) starfish powder is prepared：The starfish after impurity dries will be removed and be crushed to 100 mesh, obtain starfish powder；
B) irradiation starfish powder is prepared：Starfish powder is placed under the far infrared that temperature is 180 DEG C and irradiates 40min, must be irradiated Starfish powder；
C) starfish saponin liquid is prepared:Will irradiation starfish powder, ethyl alcohol and ether it is miscible after, be added in flash extracter in temperature Spend be 10~25 DEG C under conditions of extract 5min, the mass ratio of starfish powder, ethyl alcohol and ether is 1:18:2, mistake after extraction Filter, gained filtrate is starfish saponin liquid；
The extracts of Chinese herbal medicine is trifoliate jewelvine extracting solution and Herba Houttuyniae extract, and extracting method is according to following steps It carries out：
1) not good liquor is prepared：By one kind in trifoliate jewelvine or cordate houttuynia and water according to mass ratio 1:15 are added in container, add Heat is cooled to 30 DEG C, filtering, gained filtrate is a not good liquor to 65 DEG C, constant temperature 40min；Residue obtained is the dregs of a decoction；
2) two not good liquors are prepared：By the dregs of a decoction and water according to mass ratio 1:12 are added in container, are heated to 95 DEG C, are in pressure Under the conditions of 1.5MPa, constant temperature 45min is cooled to 30 DEG C, and filtering, gained filtrate is two not good liquors；
3) extracting solution is prepared：Two not good liquors obtained by a not good liquor obtained by step 1) and step 2) are merged, are 75 DEG C in temperature, pressure Under the conditions of power is -0.8MPa, 30min is concentrated, gained concentrate is one kind in trifoliate jewelvine or Herba Houttuyniae extract.
Self-prepared organic fertilizer follows the steps below：
1) fermentate, is prepared：By remaining leftover bits and pieces in the animal wastes in beast fowl cultivation garden, biogas residue, product processing garden processing Material, urea, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose are according to mass ratio 80:5:10:2:0.5:0.5:1:1 is added to Mixing in the fermentation tank to sterilize, it is 65% to be adjusted to water content with water, obtains mixed culture medium, accesses candida utili, produces protein The mass ratio of Candida and mixed culture medium is 0.5:99.5, it is 28 DEG C in temperature, under the conditions of oxygen content is 0.2mg/L, hair Ferment 5 days, sterilize 20min under the conditions of being 120 DEG C in temperature, obtains fermentate；
2) ammonia-producing bacterium fermentate, is prepared：Corynebacterium ammoniagenes prepared by thawing method, fermentate and thawing are accessed in fermentate The mass ratio of corynebacterium ammoniagenes prepared by method is 98:2, it is 30 DEG C in temperature, water content 60%, under the conditions of oxygen content is 3mg/L, 5 days are fermented to get production ammoniacal fermentation object；
3) organic fertilizer, is prepared：Ammoniacal fermentation object is produced after being granulated, drying, cool down and sieve to get organic fertilizer.
The preparation of thawing method corynebacterium ammoniagenes follows the steps below：
A) expansion of strain is further cultured for：Using the thalline in exponential phase as seed, it is inoculated with 10% inoculum concentration In fluid nutrient medium, 48h is cultivated in 30 DEG C of shaking table 160r/min, after 8000r/min centrifuges 10min, expansion is obtained and is further cultured for Strain；
B) thawing method improves the permeability of cell：Thawing method is at least repeated once, and step is：The expansion that step 1) is obtained It is further cultured for strain greatly to be put into -20 DEG C of refrigerator, freezes 4h, take out, dissolve at room temperature；
PH7.2~7.4 of the fluid nutrient medium, and be made into according to following mass ratio：Beef infusion broth 1.0L, peptone 10.0g, NaCl 5.0g, agar 25.0g；
The preparation method of wherein beef infusion broth follows the steps below：Lean beef is cleaned, and chopping weighs 550 grams of immersions It in 1375ml water, impregnates a night, filtering, 0.6kg/cm after filtrate packing2Sterilize 40min.
Embodiment 2
The characteristic kind cultivation villa garden circulation pattern includes plantation；
Tourist, which enters plantation, can watch and pick the economic plants planted in plantation, and economic plants has rape, white Dish, peach, cherry tree, strawberry, herbage, purslane and Herba Euphorbiae Humifusae；
The plantation is irrigated using trickle irrigation；Fertilizer uses organic fertilizer, biological organic fertilizer and self-prepared organic fertilizer；Pollination uses Hornet and spontaneous pollination；Insect prevention using plantation garlic, lavender, fish pelargonium, common nepenthes and shichongcao and put in a suitable place to breed coccinella septempunctata, The orange spider dragonfly of pyriform, Chrysopa, ichneumon wasp and the grazing eclipse acarid in trichogramma and the Holy Land；Diseases prevention is using self-control biological pesticide, sulphur Mixture, Bordeaux mixture, mineral oil and trichoderma harzianum.
Self-control biological pesticide is made according to following steps：
By starfish saponin liquid, extracts of Chinese herbal medicine, auxiliary agent and vegetable oil according to mass ratio 25:25:15:35 be added to it is homogeneous In machine, homogeneous 2 times, wherein first time homogeneous pressure is 30MPa, and homogeneous temperature is 60 DEG C, and the homogeneous time is 20min；Second Homogeneous pressure is 4PMa, and homogeneous temperature is 60 DEG C, and the homogeneous time is 30min to get biological pesticide；
The starfish saponin liquid follows the steps below：
A) starfish powder is prepared：The starfish after impurity dries will be removed and be crushed to 100 mesh, obtain starfish powder；
B) irradiation starfish powder is prepared：Starfish powder is placed under the far infrared that temperature is 180 DEG C and irradiates 40min, must be irradiated Starfish powder；
C) starfish saponin liquid is prepared:Will irradiation starfish powder, ethyl alcohol and ether it is miscible after, be added in flash extracter in temperature Spend be 10~25 DEG C under conditions of extract 5min, the mass ratio of starfish powder, ethyl alcohol and ether is 1:18:2, mistake after extraction Filter, gained filtrate is starfish saponin liquid；
The extracts of Chinese herbal medicine is trifoliate jewelvine extracting solution and Herba Houttuyniae extract, and extracting method is according to following steps It carries out：
1) not good liquor is prepared：By one kind in trifoliate jewelvine or cordate houttuynia and water according to mass ratio 1:15 are added in container, add Heat is cooled to 30 DEG C, filtering, gained filtrate is a not good liquor to 65 DEG C, constant temperature 40min；Residue obtained is the dregs of a decoction；
2) two not good liquors are prepared：By the dregs of a decoction and water according to mass ratio 1:12 are added in container, are heated to 95 DEG C, are in pressure Under the conditions of 1.5MPa, constant temperature 45min is cooled to 30 DEG C, and filtering, gained filtrate is two not good liquors；
3) extracting solution is prepared：Two not good liquors obtained by a not good liquor obtained by step 1) and step 2) are merged, are 75 DEG C in temperature, pressure Under the conditions of power is -0.8MPa, 30min is concentrated, gained concentrate is one kind in trifoliate jewelvine or Herba Houttuyniae extract.
Self-prepared organic fertilizer follows the steps below：
1) fermentate, is prepared：By animal wastes, urea, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose according to matter Measure ratio 95:2:0.5:0.5:1:1 mixing, it is 65% to be adjusted to water content with water, obtains mixed culture medium, accesses candida utili, The mass ratio of candida utili and mixed culture medium is 0.5:99.5, it stacks, ferments 20 days, obtain fermentate；
2) ammonia-producing bacterium fermentate, is prepared：Corynebacterium ammoniagenes prepared by thawing method, fermentate and thawing are accessed in fermentate The mass ratio of corynebacterium ammoniagenes prepared by method is 98:2, it is 30 DEG C in temperature, under the conditions of water content 60%, ferments 5 days to get production Ammoniacal fermentation object；
3) organic fertilizer, is prepared：Ammoniacal fermentation object is produced after being granulated, drying, cool down and sieve to get organic fertilizer.
The preparation of thawing method corynebacterium ammoniagenes follows the steps below：
A) expansion of strain is further cultured for：Using the thalline in exponential phase as seed, it is inoculated with 10% inoculum concentration In fluid nutrient medium, 48h is cultivated in 30 DEG C of shaking table 160r/min, after 8000r/min centrifuges 10min, expansion is obtained and is further cultured for Strain；
B) thawing method improves the permeability of cell：Thawing method is at least repeated once, and step is：The expansion that step 1) is obtained It is further cultured for strain greatly to be put into -20 DEG C of refrigerator, freezes 4h, take out, dissolve at room temperature；
PH7.2~7.4 of the fluid nutrient medium, and be made into according to following mass ratio：Beef infusion broth 1.0L, peptone 10.0g, NaCl 5.0g, agar 25.0g；
The preparation method of wherein beef infusion broth follows the steps below：Lean beef is cleaned, and chopping weighs 550 grams of immersions It in 1375ml water, impregnates a night, filtering, 0.6kg/cm after filtrate packing2Sterilize 40min.
Embodiment 3
The characteristic kind cultivation villa garden circulation pattern includes plantation, beast fowl cultivation garden, aquaculture garden, food and drink garden, stops Not busy amusement garden and product processing garden；
Tourist, which enters plantation, can watch and pick the economic plants planted in plantation, and economic plants has rape, white Dish, peach, cherry tree, strawberry, herbage, purslane and Herba Euphorbiae Humifusae；
Tourist, which enters beast fowl cultivation garden, can watch and buy the beast fowl cultivated in beast fowl cultivation garden, beast fowl have chicken, duck, Goose, pig, ox, Ma Heyang；
Tourist, which enters aquaculture garden, can watch the aquatic products cultivated in aquaculture garden of fishing, aquatic products have carp, Grass carp, crab methyl red, prawn, grass shrimp, cray, scallop and tortoise；
The food and drink garden is used to provide food and drink and lodging for tourist；
Tourist can carry out Drifting, hot spring bathing, trampoline, dodgem and Rollercoaster amusement in amusement and recreation garden and live It is dynamic；
The product processing garden is used for food processing, brewing, brewed spirit, wine production, dairy produce and fruit juice Manufacture, tourist can buy according to oneself demand；
The processed rear formation feed of straw and fruit that plantation generates, for the brutish kind in beast fowl cultivation garden And the nursing of the aquatic products in aquaculture garden；Organic fertilizer is formed after the metabolic waste of brutish kind is processed in beast fowl cultivation garden Or biological organic fertilizer or feed, for supplying the nutriment needed for the economic plants in plantation and the aquatic products in aquaculture garden Feed；The mud of aquaculture garden bottom of pond can be used in plantation as nutriment, amusement and recreation garden and aquaculture garden Animal drinks in irrigation and beast fowl cultivation garden after the filtered disinfection of water available for botanical garden.
The plantation is irrigated using trickle irrigation；Fertilizer uses beast poultry manure in beast fowl cultivation garden, biogas residue, product processing garden to add Organic fertilizer or biological organic fertilizer is made in remaining leftover bits and pieces in work；Pollination is using hornet and spontaneous pollination；Insect prevention is using plantation Garlic, lavender, fish pelargonium, common nepenthes and shichongcao and put in a suitable place to breed the orange spider dragonfly of coccinella septempunctata, pyriform, Chrysopa, ichneumon wasp and Trichogramma and the grazing eclipse acarid in the Holy Land；Diseases prevention is using self-control biological pesticide, sulphur mixture, Bordeaux mixture, mineral oil and Ha Ci wood Mould.
Characteristic kind cultivation villa garden circulation pattern further includes methane-generating pit, and the methane-generating pit is given birth to using the metabolic waste that animal generates Into biogas and biogas residue, the biogas is plantation, beast fowl cultivates garden, aquaculture garden, food and drink garden, amusement and recreation garden and product and adds Work garden provides the energy, and the biogas residue is used for the industrial crops in breeding and planting garden and the aquatic products in aquaculture garden.
The plantation, beast fowl cultivation garden, aquaculture garden, food and drink garden, amusement and recreation garden and product processing garden at the top of with And several solar panels are installed on campus portion vacant lot, the solar panel is connected with header box, and passes through institute State header box and be uniformly connected to DC cabinet, the DC cabinet connects inverter, the inverter after electric quantity metering device, and Enter national grid.
Self-control biological pesticide is made according to following steps：
By starfish saponin liquid, extracts of Chinese herbal medicine, auxiliary agent and vegetable oil according to mass ratio 25:25:15:35 be added to it is homogeneous In machine, homogeneous 1 time, homogeneous pressure 35MPa, homogeneous temperature is 60 DEG C, and the homogeneous time is 30min to get biological pesticide；
The starfish saponin liquid follows the steps below：
A) starfish powder is prepared：The starfish after impurity dries will be removed and be crushed to 100 mesh, obtain starfish powder；
B) irradiation starfish powder is prepared：Starfish powder is placed under the far infrared that temperature is 200 DEG C and irradiates 30min, must be irradiated Starfish powder；
C) starfish saponin liquid is prepared:Will irradiation starfish powder and ethyl alcohol it is miscible after, be added in flash extracter and be in temperature Extract 10min under conditions of 10~25 DEG C, the mass ratio of starfish powder and ethyl alcohol is 1:25, it is filtered after extraction, gained filtrate As starfish saponin liquid；
The extracts of Chinese herbal medicine is trifoliate jewelvine extracting solution and Herba Houttuyniae extract, and extracting method is according to following steps It carries out：
1) not good liquor is prepared：By one kind in trifoliate jewelvine or cordate houttuynia and water according to mass ratio 1:20 are added in container, add Heat is cooled to 30 DEG C, filtering, gained filtrate is a not good liquor to 70 DEG C, constant temperature 30min；Residue obtained is the dregs of a decoction；
2) two not good liquors are prepared：By the dregs of a decoction and water according to mass ratio 1:15 are added in container, are heated to 95 DEG C, are in pressure Under the conditions of 1.5MPa, constant temperature 45min is cooled to 30 DEG C, and filtering, gained filtrate is two not good liquors；
3) extracting solution is prepared：Two not good liquors obtained by a not good liquor obtained by step 1) and step 2) are merged, are 75 DEG C in temperature, item Under part, 40min is concentrated, gained concentrate is one kind in trifoliate jewelvine or Herba Houttuyniae extract.
Self-prepared organic fertilizer follows the steps below：
1) fermentate, is prepared：By remaining leftover bits and pieces in the animal wastes in beast fowl cultivation garden, biogas residue, product processing garden processing Material, urea, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose are according to mass ratio 75:10:10:2:0.5:0.5:1:1 adds in The mixing into the fermentation tank to sterilize, it is 65% to be adjusted to water content with water, obtains mixed culture medium, accesses candida utili, production The mass ratio of protein Candida and mixed culture medium is 1:99, it is 28 DEG C in temperature, under the conditions of oxygen content is 0.2mg/L, fermentation 5 My god, sterilize 20min under the conditions of being 120 DEG C in temperature, obtains fermentate；
2) ammonia-producing bacterium fermentate, is prepared：Corynebacterium ammoniagenes prepared by thawing method, fermentate and thawing are accessed in fermentate The mass ratio of corynebacterium ammoniagenes prepared by method is 98:2, it is 30 DEG C in temperature, water content 60%, under the conditions of oxygen content is 3mg/L, 5 days are fermented to get production ammoniacal fermentation object；
3) organic fertilizer, is prepared：Ammoniacal fermentation object is produced after being granulated, drying, cool down and sieve to get organic fertilizer.
The preparation of thawing method corynebacterium ammoniagenes follows the steps below：
A) expansion of strain is further cultured for：Using the thalline in exponential phase as seed, it is inoculated with 10% inoculum concentration In fluid nutrient medium, 48h is cultivated in 30 DEG C of shaking table 160r/min, after 8000r/min centrifuges 10min, expansion is obtained and is further cultured for Strain；
B) thawing method improves the permeability of cell：Thawing method is at least repeated once, and step is：The expansion that step 1) is obtained It is further cultured for strain greatly to be put into -20 DEG C of refrigerator, freezes 4h, take out, dissolve at room temperature；
PH7.2~7.4 of the fluid nutrient medium, and be made into according to following mass ratio：Beef infusion broth 1.0L, peptone 10.0g, NaCl 5.0g, agar 25.0g；
The preparation method of wherein beef infusion broth follows the steps below：Lean beef is cleaned, and chopping weighs 550 grams of immersions It in 1375ml water, impregnates a night, filtering, 0.6kg/cm after filtrate packing2Sterilize 40min.
The advantageous effect further illustrated the present invention with reference to experimental data：
1st, material to be tested is tested
1 materials and methods：
1.1 test site：Yantai Qixia City Su Jiadian towns apple cultivation garden.
1.2 material to be tested：Common orchard (in addition to not planting expelling parasite plant, putting beneficial insect and acarid in a suitable place to breed, other management with Embodiment 2 is consistent), comparison 1 (when preparing organic fertilizer, in addition to corynebacterium ammoniagenes are without thawing method processing directly use, other management It is consistent with embodiment 2) and embodiment 2, it compares.
This experiment is consistent with other management in addition to processing difference except testing.
1.3 experimental design：The apple orchard for selecting 6 mu of age of trees consistent, 2 is parallel, and 1 mu is 1 processing unit.For common orchard (in addition to not planting expelling parasite plant, putting beneficial insect and acarid in a suitable place to breed, other management are consistent with embodiment 2), comparison 1 (are prepared organic It is other to manage with embodiment 2 unanimously except corynebacterium ammoniagenes are without the processing of thawing method directly in addition to use when fertile) and embodiment 2 do Orchard insect pest situation is observed in comparison, counts orchard apple production.
2 results and analysis
Apple average product (kg) Orchard insect pest situation
Common orchard 1856.7 Insect pest is serious
Comparison 1 1976.5 Substantially without worm
Embodiment 2 2037.4 Substantially without worm
It is compared by common orchard with the present invention, it can be seen that the present invention has apparent anthelminthic effect, is capable of providing apple Yield；Being compared by embodiment 2 and comparison 1 can show that corynebacterium ammoniagenes through thawing method high treating effect, can improve apple production.

Claims (10)

1. a kind of ecological agriculture leisure picking management mode, which is characterized in that cultivate villa garden circulation pattern including characteristic kind；
The characteristic kind cultivation villa garden circulation pattern includes plantation, beast fowl cultivation garden, aquaculture garden, food and drink garden, leisure joy One in paradise and product processing garden or arbitrarily combine two or more；
The plantation is used to plant economic plants；
The beast fowl cultivation garden is used to cultivate beast fowl；
The aquaculture garden is used for aquatic kind；
The food and drink garden is used for one or two in providing food and drink for tourist and stay；
The amusement and recreation garden is used to provide one in aquatic recreational amenities, ground recreation and aerial recreation for tourist Or arbitrarily combine two or more；
The product processing garden is used to support the economic plants in the plantation, the beast fowl kind in beast fowl cultivation garden, aquatic products It grows the aquatic products in garden and is processed sale and the brewing of drinks；
The processed rear brutish kind and aquatic products available in beast fowl cultivation garden of straw and fruit that the plantation generates Aquatic products in cultivation garden；In beast fowl cultivation garden the metabolic waste of brutish kind can be used for economic plants in breeding and planting garden and Aquatic products in aquaculture garden；The mud of aquaculture garden bottom of pond can be used in plantation as nutriment, amusement and recreation garden With after the filtered disinfection of water in aquaculture garden garden is cultivated available for botanical garden and beast fowl.
2. the ecological agriculture leisure picking management mode as described in claim 1, which is characterized in that the plantation is irrigated and adopted Use trickle irrigation；Fertilizer uses self-prepared organic fertilizer or biological organic fertilizer；Pollination is using one or both of hornet and spontaneous pollination Combination；Insect prevention is using plant of the plantation with anthelminthic effect and puts the combination of one or both of beneficial insect and acarid in a suitable place to breed；It is anti- Disease is using self-control biological pesticide, sulphur mixture, Bordeaux mixture, mineral oil and one kind in trichoderma harzianum or arbitrarily combine two kinds More than；The plant of the anthelminthic effect is one kind or arbitrary in garlic, lavender, fish pelargonium, common nepenthes, shichongcao and folium artemisiae argyi It combines two or more；The beneficial insect is one in coccinella septempunctata, the orange spider dragonfly of pyriform, Chrysopa, ichneumon wasp and trichogramma Kind arbitrarily combines two or more；The beneficial acarid is the grazing eclipse acarid in the Holy Land.
3. the ecological agriculture leisure picking management mode as described in claim 1, which is characterized in that the plantation is for kind It plants industrial crops and includes one or more of vegetables, fruit tree, herbage and Chinese medicine；
The beast fowl cultivation garden is used to cultivate one or more of chicken, duck, goose, pig, ox and sheep；
The aquaculture garden is for one or more of cultured fishes, crab class, shrimps, shell and Chelonian；
The food and drink garden is used for using the industrial crops in plantation, the beast fowl in beast fowl cultivation garden, in aquaculture garden The drinks of the food and brewing of aquatic products and product processing garden processing provide food and beverage sevice；
The amusement and recreation garden is for offer swimming, hot spring bathing, trampoline, Wild Mouse, carrousel, roller-coaster and dodgem In one or arbitrarily combine two or more；
The product processing garden is for food processing, brewing, brewed spirit, wine production, dairy produce and fruit juice manufacture One or more of.
4. ecological agriculture leisure picking management mode as described in claim 1, which is characterized in that including methane-generating pit, the natural pond Gas pond is formed methane using the metabolic waste that animal generates and biogas residue, and the biogas is plantation, beast fowl cultivates garden, aquaculture Garden, food and drink garden, amusement and recreation garden and product processing garden provide the energy, the biogas residue be used for breeding and planting garden in industrial crops and Aquatic products in aquaculture garden.
5. ecological agriculture leisure picking management mode as described in claim 1, which is characterized in that the plantation, beast fowl are supported It grows at the top of garden, aquaculture garden, food and drink garden, amusement and recreation garden and product processing garden and is installed on campus portion vacant lot several Solar panel, the solar panel are connected with header box, and pass through the header box and be uniformly connected to DC cabinet, institute DC cabinet connection inverter is stated, the inverter is incorporated to national grid after electric quantity metering device.
6. the ecological agriculture as claimed in claim 2 leisure picking management mode, which is characterized in that self-control biological pesticide according to Lower step is made：
By starfish saponin liquid, extracts of Chinese herbal medicine, auxiliary agent and vegetable oil according to mass ratio 20~30:20~30:10~20:30~ 50 are added in homogenizer, at least homogeneous once to get biological pesticide；
The starfish saponin liquid follows the steps below：
A) starfish powder is prepared：The starfish after impurity dries will be removed and be crushed to 80~200 mesh, obtain starfish powder；
B) irradiation starfish powder is prepared：Starfish powder is placed in 20~100min of irradiation under far infrared, starfish powder must be irradiated；
c）Prepare starfish saponin liquid:Will irradiation starfish powder and solvent it is miscible after, be added in flash extracter, temperature for 5~ 30℃
Under the conditions of extract, the mass ratio of starfish powder and solvent is 1:9~40, it is filtered after extraction, gained filtrate is starfish Saponin(e liquid；The extraction voltage of the homogenate extraction be 100~150V, rotating speed be 5000~10000rpm/min, extraction time For 3~20min；
The auxiliary agent be two kinds of the one or any ratio in wetting agent, dispersant, stabilizer, sustained release agent and synergist with On；
The temperature condition of the radiation treatment is 150~250 DEG C；
The solvent is the two or more of the one or any ratio in ethyl alcohol, methanol, methyl acetate and ether；
The vegetable oil is the two of palm oil, coconut oil, chinaberry seeds oil, mandarin orange oil and the one or any ratio in fruit of cubeb litsea tree oil Kind or more；
The extracts of Chinese herbal medicine is trifoliate jewelvine extracting solution and Herba Houttuyniae extract, extracting method be according to following steps into Row：
1）Prepare a not good liquor：By Chinese herbal medicine and water according to mass ratio 1:5~40 are added in container, are heated to 50~75 DEG C, perseverance 20~90min of temperature is cooled to 20~40 DEG C, and filtering, gained filtrate is a not good liquor；Residue obtained is the dregs of a decoction；
2）Prepare two not good liquors：By the dregs of a decoction and water according to mass ratio 1:5~40 are added in container, are heated to 70~120 DEG C, are pressing Under the conditions of power is 0~5MPa, 20~90min of constant temperature is cooled to 20~40 DEG C, and filtering, gained filtrate is two not good liquors；
3）Prepare extracting solution：By step 1）One not good liquor of gained and step 2）Two not good liquor of gained merges, and is 20~90 DEG C in temperature, pressure Under the conditions of power is -5MPa~0MPa, 0~90min is concentrated, gained concentrate is extracting solution.
7. ecological agriculture leisure picking management mode as claimed in claim 6, which is characterized in that homogeneous 2 times, wherein for the first time Homogeneous pressure is 15~35MPa, and homogeneous temperature is 50~70 DEG C, and the homogeneous time is 10~60min；Second of homogeneous pressure is 2 ~8PMa, homogeneous temperature are 50~70 DEG C, and the homogeneous time is 10~60min.
8. ecological agriculture leisure picking management mode as claimed in claim 6, which is characterized in that step b）Described in irradiation Temperature is 180 DEG C, and exposure time is 40min.
9. ecological agriculture leisure picking management mode as claimed in claim 6, which is characterized in that self-prepared organic fertilizer is according to following Step carries out：
1）, prepare fermentate：By remaining leftover bits and pieces, urine in the animal wastes in beast fowl cultivation garden, biogas residue, product processing garden processing Element, magnesium sulfate, sodium chloride, potassium dihydrogen phosphate and glucose are according to mass ratio 30~99:0~30:0~30:0~4:0~1:0~ 2:0~4:0~4 is added to mixing in the fermentation tank to sterilize, and it is 50~70% to be adjusted to water content with water, obtains mixed culture medium, connects Enter candida utili, the mass ratio of candida utili and mixed culture medium is 0.2~2:98~99.8, temperature for 25~ 35 DEG C, under the conditions of oxygen content is 0.1~0.5mg/L, ferment 2~7 days, temperature for sterilizing 20 under the conditions of 70~150 DEG C~ 90min obtains fermentate；
2）, prepare ammonia-producing bacterium fermentate：Corynebacterium ammoniagenes prepared by thawing method, fermentate and thawing legal system are accessed in fermentate The mass ratio of standby corynebacterium ammoniagenes is 95~99:1~5, it is 25~35 DEG C in temperature, water content 50~70%, oxygen content 1 Under the conditions of~5mg/L, 2~7 days are fermented to get production ammoniacal fermentation object；
3）, prepare organic fertilizer：Ammoniacal fermentation object is produced after being granulated, drying, cool down and sieve to get organic fertilizer.
10. the preparation method of organic fertilizer as claimed in claim 2, it is characterised in that：The preparation of thawing method corynebacterium ammoniagenes is pressed It is carried out according to following steps：
a）The expansion of strain is further cultured for：Using the thalline in exponential phase as seed, liquid is inoculated in 10% inoculum concentration In culture medium, 48h is cultivated in 30 DEG C of shaking table 160r/min, after 8000r/min centrifuges 10min, expansion is obtained and is further cultured for strain；
b）Thawing method improves the permeability of cell：Thawing method is at least repeated once, and step is：By step 1）Obtained expansion is again Culture strain is put into -20 DEG C of refrigerator, freezes 4h, is taken out, is dissolved at room temperature；
PH7.2~7.4 of the fluid nutrient medium, and be made into according to following mass ratio：Beef infusion broth 1.0L, peptone 10.0g, NaCl 5.0g, agar 25.0g;
The preparation method of wherein beef infusion broth follows the steps below：Lean beef is cleaned, and chopping weighs 550 grams and is soaked in It in 1375ml water, impregnates a night, filtering, 0.6kg/cm after filtrate packing2Sterilize 40min.
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