CN108181946B - Ice bath device for detection room and use method thereof - Google Patents
Ice bath device for detection room and use method thereof Download PDFInfo
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- CN108181946B CN108181946B CN201711494293.3A CN201711494293A CN108181946B CN 108181946 B CN108181946 B CN 108181946B CN 201711494293 A CN201711494293 A CN 201711494293A CN 108181946 B CN108181946 B CN 108181946B
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- ice bath
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- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
- G05D23/00—Control of temperature
- G05D23/19—Control of temperature characterised by the use of electric means
- G05D23/20—Control of temperature characterised by the use of electric means with sensing elements having variation of electric or magnetic properties with change of temperature
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Abstract
The invention discloses an ice bath device for a detection room and a using method thereof, wherein the ice bath device comprises a shell, a sealing cover, a pot body, a sample fixing mechanism, a rotating mechanism and two groups of lifting mechanisms; a plurality of mutually independent ice bath tanks are arranged in the pot body, and each ice bath tank comprises a refrigeration chip, a temperature sensor and a temperature indicator light; the rotating mechanism comprises a first motor and a cam divider; each lifting mechanism comprises a second motor and a magnet block; the control panel comprises a plurality of groups of regulating units and a PLC controller, and the regulating units, the refrigeration chip, the temperature sensor, the temperature indicator lamp, the supporting piece, the first motor, the second motor and the electromagnet are respectively electrically connected with the PLC controller. The automatic ice bath machine can set different ice bath temperatures at the same time, the pot body can rotate automatically, the test tube in the fixed disc is immersed into the designated ice bath groove for ice bath, the automatic operation is realized, the requirements of reagents on different ice bath conditions are met, and the pollution problem caused by manual operation is avoided.
Description
Technical Field
The invention belongs to the technical field of water baths, and particularly relates to an ice bath device for a detection room.
Background
The ice bath is one of the common means for organic synthesis tests and biochemical tests, and can protect samples in the biochemical tests from deterioration through the ice bath. The ice bath method is a method for reducing the temperature by using ice cubes, wherein the ice cubes are placed in a large container, a reaction container is placed in the ice cubes, and the reaction temperature is reduced by the ice cubes, so that the purpose of reducing the temperature is achieved. Chinese patent CN 206715978U discloses "an ice bath device for cell disruption", which is provided with a large beaker, four rubber belts are arranged at the opening at the upper end of the large beaker in a well shape, a rubber pad is placed in the middle of the large beaker, a small beaker is placed at the upper end of the rubber pad, and the upper end of the small beaker is arranged in the four rubber belts; an ice-water mixture is filled between the large beaker and the small beaker, and a thermometer is fixedly arranged on the inner wall of the small beaker through a fixing ring; the small beaker is filled with a solution to be tested. The device has alleviated the vibrations of little beaker through the installation rubber pad, through set up the heat preservation beaker cover in the bottom of big beaker, can prevent that temperature variation is too violent.
However, for some samples, the activity of the samples can be recovered only after ice bath at different temperatures, the existing ice bath device can only set a uniform temperature, if the samples are subjected to ice bath at other water temperatures, the test tube needs to be manually moved to another ice bath device, the change of the temperature in the moving process can affect the biological activity of the samples, and the samples can be polluted due to manual movement for many times.
Disclosure of Invention
The invention mainly solves the technical problem of providing an ice bath device for a detection chamber and a using method thereof, different ice bath temperatures can be set simultaneously, a pot body can rotate autonomously, a test tube in a fixed disc is immersed in a specified ice bath groove for ice bath, automatic operation is realized, the requirements of reagents under different ice bath conditions are met, and the pollution problem caused by manual operation is avoided.
In order to solve the technical problems, the invention adopts a technical scheme that: an ice bath device for a detection room comprises a shell, a sealing cover, a pot body, a sample fixing mechanism, a rotating mechanism and two groups of lifting mechanisms, wherein the sealing cover is positioned above the shell, the pot body is positioned in the shell, the two groups of lifting mechanisms and the rotating mechanism are positioned between the shell and the pot body, and the two groups of lifting mechanisms are symmetrically positioned at the bottom of the pot body;
the ice bath device is characterized in that a plurality of mutually independent ice bath grooves are formed in the pot body, each ice bath groove comprises a refrigeration chip, a temperature sensor and a temperature indicator lamp, the refrigeration chip is arranged at the bottom of the ice bath groove, the temperature sensor is fixed on the inner wall of the ice bath groove, and the temperature indicator lamp is positioned on the outer wall of the ice bath groove;
the sample fixing mechanism comprises a fixed disc and a supporting piece, one end of the supporting piece is fixedly connected with the fixed disc, and the other end of the supporting piece is fixed on one side of the shell;
the rotating mechanism comprises a first motor and a cam divider, the center of the pot body is connected with the output end of the cam divider, the input end of the cam divider is connected with the first motor, and the first motor is fixed at the bottom of the shell;
each lifting mechanism comprises a second motor and a magnet block fixed at the front end of an output shaft of the second motor, two electromagnets are arranged at the bottom of the pot body and symmetrically arranged, and the electromagnets correspond to the magnet blocks in position;
the control panel comprises a plurality of groups of regulating units and a PLC (programmable logic controller), each regulating unit correspondingly regulates and controls each ice bath, the regulating units, the refrigeration chip, the temperature sensor, the temperature indicator lamp, the supporting piece, the first motor, the second motor and the electromagnet are respectively and electrically connected with the PLC, and the PLC is externally connected with a power supply;
an elastic connecting piece is arranged between the first motor and the shell.
Furthermore, the regulation and control unit comprises a temperature adjusting key, a time adjusting key and a display screen, wherein the temperature adjusting key, the time adjusting key and the display screen are respectively and electrically connected with the PLC.
Further, the number of the ice bath grooves is two, and the two ice bath grooves are of semicircular structures.
Furthermore, the fixed disk comprises a plurality of fixing pieces, two ends of each fixing piece are respectively fixed with the inner wall of the fixed disk, and the plurality of fixing pieces are mutually staggered to form a net-shaped structure.
Further, the fixing piece is a stainless steel spring fixing piece.
Further, the elastic connecting piece is a rigid spring.
Further, the fixed disk is a circular fixed disk, and the diameter of the fixed disk is smaller than the radius of the ice bath.
Further, the first motor is a stepping motor.
Further, the second motor is a lifting cylinder.
The use method of the ice bath device for the detection room comprises the following steps:
the method comprises the following steps: starting the equipment, injecting a proper amount of liquid gel into the ice bath, setting the water temperature of the corresponding ice bath and the time needing ice bath through a temperature adjusting key and a time adjusting key on a control panel, and covering a sealing cover;
step two: the PLC controller controls the refrigeration chip to refrigerate, when the water temperature in each ice bath tank reaches a set value, the temperature sensor transmits a signal to the PLC controller, and then a temperature indicator lamp on the outer wall of each ice bath tank is lightened;
step three: placing a sample needing ice bath in a test tube, fixing the test tube between meshes formed by fixing parts on a fixing frame, driving a pot body to ascend by an output shaft of a second motor, ensuring that the test tube in the fixing disc is immersed in a designated ice bath tank, and carrying out ice bath on the sample in the test tube;
step four: when the ice bath time set value is reached, the magnet block on the output shaft of the second motor is attracted with the electromagnet at the bottom of the pot body, the pot body is driven by the second motor to descend to the original position, the magnet block is separated from the electromagnet, the first motor drives the cam divider to rotate, so that the pot body rotates to a set angle, and the second motor drives the pot body to ascend, so that the test tube is immersed in an ice bath at another temperature for ice bath;
step five: and after the ice bath is completely finished, opening the sealing cover, and taking out the test tube from the fixed disk.
The invention has the following beneficial effects:
1. the water bath kettle can set different ice bath temperatures at the same time, and meets the requirement that the activity of the reagent can be recovered only by ice baths at different temperatures; the pot body can rotate autonomously, the test tube in the fixed disc is immersed in the designated ice bath groove for ice bath, automatic operation is realized, and the pollution problem caused by manual operation is avoided;
2. the pot body is provided with a plurality of mutually independent ice bath grooves, and the ice bath temperature and time of the corresponding ice bath groove can be set through the regulating and controlling unit on the control panel, so that the requirements of reagents for passing different ice bath temperatures are met;
3. the pot body can be automatically lifted under the driving of the second motor, so that the test tube in the fixed disc is ensured to be immersed in an ice bath in a specified ice bath groove and rotated to a specified angle under the driving of the first motor, the test tube does not need to be manually moved, and reagent pollution caused by manual operation is avoided;
4. the PLC controller is used for controlling the operation process of the whole equipment, the automation degree is high, the test efficiency is improved, and the test effect is ensured.
Drawings
FIG. 1 is a schematic diagram of the apparatus of the present invention;
FIG. 2 is a schematic view of the internal structure of the housing of the present invention;
FIG. 3 is a schematic diagram of the ice bath configuration of the present invention;
FIG. 4 is a schematic view of the structure of the fixed disk of the present invention;
FIG. 5 is a control schematic of the present invention;
the parts in the drawings are marked as follows:
the device comprises a shell 1, a sealing cover 2, a pot body 3, an ice bath groove 4, a refrigeration chip 41, a temperature sensor 42, a temperature indicator lamp 43, a fixed disc 5, a fixing piece 51, a supporting piece 6, a first motor 7, a cam divider 8, a second motor 9, a magnet block 10, an electromagnet 11, a PLC (programmable logic controller) 12, a power supply 13, a temperature adjusting key 14, a time adjusting key 15, a display screen 16 and an elastic connecting piece 17.
Detailed Description
The following detailed description of the preferred embodiments of the present invention, taken in conjunction with the accompanying drawings, will make the advantages and features of the invention easier to understand by those skilled in the art, and thus will clearly and clearly define the scope of the invention.
Example (b): an ice bath device for a detection room is shown in figures 1-5 and comprises a shell 1, a sealing cover 2 positioned above the shell, a pot body 3 positioned in the shell, a sample fixing mechanism, a rotating mechanism and two groups of lifting mechanisms, wherein the two groups of lifting mechanisms and the rotating mechanism are positioned between the shell and the pot body, and the two groups of lifting mechanisms are symmetrically positioned at the bottom of the pot body;
a plurality of mutually independent ice bath grooves 4 are arranged in the pot body 3, each ice bath groove 4 comprises a refrigeration chip 41, a temperature sensor 42 and a temperature indicator lamp 43, the refrigeration chip 41 is arranged at the bottom of the ice bath groove 4, the temperature sensor 42 is fixed on the inner wall of the ice bath groove 4, and the temperature indicator lamp 43 is positioned on the outer wall of the ice bath groove 4;
the sample fixing mechanism comprises a fixing disc 5 and a supporting piece 6, one end of the supporting piece 6 is fixedly connected with the fixing disc 5, and the other end of the supporting piece 6 is fixed on one side of the shell 1;
the rotating mechanism comprises a first motor 7 and a cam divider 8, the center of the pot body 3 is connected with the output end of the cam divider 8, the input end of the cam divider 8 is connected with the first motor 7, and the first motor 7 is fixed at the bottom of the shell 1;
each lifting mechanism comprises a second motor 9 and a magnet block 10 fixed at the front end of an output shaft of the second motor, two electromagnets 11 are arranged at the bottom of the pot body 3, the two electromagnets 11 are symmetrically arranged, and the magnet block 10 corresponds to the electromagnets 11 in position;
the control panel comprises a plurality of groups of regulating units and a PLC (programmable logic controller) 12, each regulating unit correspondingly regulates and controls each ice bath, the regulating units, the refrigeration chip, the temperature sensor, the temperature indicator lamp, the supporting piece, the first motor, the second motor and the electromagnet are respectively and electrically connected with the PLC, and the PLC is externally connected with a power supply;
an elastic connecting piece 17 is arranged between the first motor 7 and the shell 1.
The regulation and control unit comprises a temperature adjusting key 14, a time adjusting key 15 and a display screen 16, wherein the temperature adjusting key, the time adjusting key and the display screen are respectively and electrically connected with the PLC.
The number of the ice bath grooves 4 is two, and the two ice bath grooves 4 are both in a semicircular structure.
The fixed disk 5 comprises a plurality of fixing pieces 51, two ends of each fixing piece 51 are respectively fixed with the inner wall of the fixed disk 5, and the plurality of fixing pieces 51 are mutually staggered to form a net-shaped structure.
The fixing member 51 is a stainless steel spring fixing member.
The elastic connecting piece 17 is a rigid spring.
The fixed disk 5 is a circular fixed disk, and the diameter of the fixed disk is smaller than the radius of the ice bath.
The first motor 7 is a stepping motor.
The second motor 9 is a lifting cylinder.
The use method of the ice bath device for the detection room comprises the following steps:
the method comprises the following steps: starting the equipment, injecting a proper amount of liquid gel into the ice bath, setting the water temperature of the corresponding ice bath and the time needing ice bath through a temperature adjusting key and a time adjusting key on a control panel, and covering a sealing cover;
step two: the PLC controller controls the refrigeration chip to refrigerate, when the water temperature in each ice bath tank reaches a set value, the temperature sensor transmits a signal to the PLC controller, and then a temperature indicator lamp on the outer wall of each ice bath tank is lightened;
step three: placing a sample needing ice bath in a test tube, fixing the test tube between meshes formed by fixing pieces on a fixing frame, driving a pot body to ascend by an output shaft of a second motor, ensuring that the test tube in the fixing disc is immersed in a specified ice bath, and carrying out ice bath on the sample in the test tube;
step four: when the ice bath time set value is reached, the magnet block on the output shaft of the second motor is attracted with the electromagnet at the bottom of the pot body, the pot body is driven by the second motor to descend to the original position, the magnet block is separated from the electromagnet, the first motor drives the cam divider to rotate, so that the pot body rotates to a set angle, and the second motor drives the pot body to ascend, so that the test tube is immersed in an ice bath at another temperature for ice bath;
step five: and after all ice baths are finished, opening the sealing cover, and taking out the test tube from the fixed disk.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all equivalent structural changes made by using the contents of the present specification and the drawings, or applied directly or indirectly to other related technical fields, are included in the scope of the present invention.
Claims (8)
1. An ice bath device for a detection room comprises a shell (1), and is characterized in that: the device also comprises a sealing cover (2) positioned above the shell, a pot body (3) positioned in the shell, a sample fixing mechanism, a rotating mechanism and two groups of lifting mechanisms, wherein the two groups of lifting mechanisms and the rotating mechanism are positioned between the shell and the pot body, and the two groups of lifting mechanisms are symmetrically positioned at the bottom of the pot body;
a plurality of ice bath grooves (4) which are mutually independent are arranged in the pot body, each ice bath groove comprises a refrigeration chip (41), a temperature sensor (42) and a temperature indicator lamp (43), the refrigeration chip is arranged at the bottom of the ice bath groove, the temperature sensor is fixed on the inner wall of the ice bath groove, and the temperature indicator lamp is positioned on the outer wall of the ice bath groove;
the sample fixing mechanism comprises a fixed disc (5) and a supporting piece (6), one end of the supporting piece is fixedly connected with the fixed disc, and the other end of the supporting piece is fixed on one side of the shell;
the rotating mechanism comprises a first motor (7) and a cam divider (8), the center of the pot body is connected with the output end of the cam divider, the input end of the cam divider is connected with the first motor, and the first motor is fixed at the bottom of the shell;
each lifting mechanism comprises a second motor (9) and a magnet block (10) fixed at the front end of an output shaft of the second motor, two electromagnets (11) are arranged at the bottom of the pot body and symmetrically arranged, and the electromagnets correspond to the magnet blocks in position;
the control panel comprises a plurality of groups of regulating units and a PLC (programmable logic controller) (12), each regulating unit correspondingly regulates and controls each ice bath, the regulating units, the refrigeration chip, the temperature sensor, the temperature indicator lamp, the supporting piece, the first motor, the second motor and the electromagnet are respectively and electrically connected with the PLC, and the PLC is externally connected with a power supply (13);
an elastic connecting piece (17) is arranged between the first motor and the shell;
the number of the ice bath grooves is two, and the two ice bath grooves are of semicircular structures;
the elastic connecting piece is a rigid spring.
2. An ice bath apparatus for a detection chamber according to claim 1, characterized in that: the regulation and control unit comprises a temperature adjusting key (14), a time adjusting key (15) and a display screen (16), wherein the temperature adjusting key, the time adjusting key and the display screen are respectively electrically connected with the PLC.
3. An ice bath apparatus for a detection chamber according to claim 1, wherein: the fixed disk comprises a plurality of fixing pieces (51), two ends of each fixing piece are respectively fixed with the inner wall of the fixed disk, and the fixing pieces are mutually staggered to form a net-shaped structure.
4. An ice bath apparatus for a detection chamber according to claim 3, wherein: the fixing piece is a stainless steel spring fixing piece.
5. An ice bath apparatus for a detection chamber according to claim 1, wherein: the fixed disk is a circular fixed disk, and the diameter of the fixed disk is smaller than the radius of the ice bath.
6. An ice bath apparatus for a detection chamber according to claim 1, wherein: the first motor is a stepping motor.
7. An ice bath apparatus for a detection chamber according to claim 1, characterized in that: the second motor is a lifting cylinder.
8. The use method of the ice bath device for the detection room according to claim 1 is characterized in that: the method comprises the following steps:
the method comprises the following steps: starting the equipment, injecting a proper amount of liquid gel into the ice bath, setting the water temperature of the corresponding ice bath and the time needing ice bath through a temperature adjusting key and a time adjusting key on a control panel, and covering a sealing cover;
step two: the PLC controller controls the refrigeration chip to refrigerate, when the water temperature in each ice bath tank reaches a set value, the temperature sensor transmits a signal to the PLC controller, and then a temperature indicator lamp on the outer wall of each ice bath tank is lightened;
step three: placing a sample needing ice bath in a test tube, fixing the test tube between meshes formed by fixing parts on a fixing frame, driving a pot body to ascend by an output shaft of a second motor, ensuring that the test tube in the fixing disc is immersed in a designated ice bath tank, and carrying out ice bath on the sample in the test tube;
step four: when the set value of ice bath time is reached, the magnet block on the output shaft of the second motor is attracted with the electromagnet at the bottom of the pot body, the pot body is driven by the second motor to descend to the original position, the magnet block is separated from the electromagnet, the first motor drives the cam divider to rotate, so that the pot body rotates to a set angle, and then the second motor drives the pot body to ascend, so that the test tube is immersed in an ice bath at another temperature for ice bath;
step five: and after the ice bath is completely finished, opening the sealing cover, and taking out the test tube from the fixed disk.
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CN201711494293.3A CN108181946B (en) | 2017-12-31 | 2017-12-31 | Ice bath device for detection room and use method thereof |
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CN108181946B true CN108181946B (en) | 2023-02-24 |
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CN112545662B (en) * | 2020-11-18 | 2022-07-12 | 顺德职业技术学院 | Self-adaptive bath specimen collecting box |
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GB1267963A (en) * | 1969-04-25 | 1972-03-22 | Knight Instr Ltd | Rotary still |
CN106645268B (en) * | 2017-02-20 | 2023-03-28 | 河南工程学院 | Freezing point measuring device |
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CN2068968U (en) * | 1990-04-27 | 1991-01-09 | 中国科学院遗传研究所 | Dna-expanding instrument for use in polymerase chain reaction |
CN204503136U (en) * | 2014-08-15 | 2015-07-29 | 金陵科技学院 | A kind of lift timing water-bath |
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