CN2068968U - Dna-expanding instrument for use in polymerase chain reaction - Google Patents
Dna-expanding instrument for use in polymerase chain reaction Download PDFInfo
- Publication number
- CN2068968U CN2068968U CN 90205278 CN90205278U CN2068968U CN 2068968 U CN2068968 U CN 2068968U CN 90205278 CN90205278 CN 90205278 CN 90205278 U CN90205278 U CN 90205278U CN 2068968 U CN2068968 U CN 2068968U
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- cam
- water bath
- cam body
- rocking bar
- electric motor
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Abstract
The utility model relates to a DNA-expanding instrument for polymerase chain reaction, belonging to the class of analytical instrument. The utility model comprises a single-board computer, a set of extraction mechanisms and three water tanks with different constant temperatures, an outer shell made of aluminum alloy is covered out of the utility model, and a panel is provided with an operation keyboard and a digital display screen. The extraction mechanisms are composed of cams, electromotors, rockers and object carriers. Biological samples are alternately soaked in the water tanks in turn via the extraction mechanisms, and soaking time and frequency and the temperature of water in the tanks are all controlled by the single-board computer. Manual labor amount is reduced, consistent experimental result is easy to obtain, and a temperature control system is simplified.
Description
The utility model relates to the polymerase chain reaction DNA(thymus nucleic acid that uses in a kind of Bioexperiment technology) the amplification instrument, belong to the analytical instrument class.
A mode that adopts amplification in vitro from micro-biological sample is obtained the technology of a large amount of specific genetic material, is that the feature of utilizing archaeal dna polymerase to depend on dna profiling is simulated intravital reproduction process bring out polyreaction between additional a pair of primer.This working process is based on sex change under three kinds of differing tempss, annealing, the extension circulation is finished.Like this, experiment needs three different constant temperature water baths of temperature, and same biological sample will soak in these three water baths according to a certain order in turn, and the time of soaking in each groove is not waited.Biological sample in each groove in turn the set(ting)value of soak time to increase progressively, such circulation will be carried out 30-50 time.If during another biological sample of experiment, then the temperature of water bath and soak time are again with preceding a kind of different.Such operation was undertaken by manual in the past, and labor capacity is big, and is loaded down with trivial details, and elapsed time is long, and poor repeatability usually influences the accuracy of experimental result.
The purpose of this utility model is will provide a kind of to control water temperature, the soak time of each groove and the operating process of soaking by sheave stream automatically by single board computer, reaches automated cycle and keep constant temperature, timing purpose accurately in each circulation.
The purpose of this utility model is achieved in that it mainly is made up of a single board computer, temperature different three constant temperature water baths and a cover extraction mechanism, and wherein extraction mechanism is by a cylindrical cam body of vertically fixedly laying, at the intravital electric motor of cam, partly form on cam body top and by motor-operated rocking bar with four of the goods racks that rocking bar connects as one.Three cams distribute on the periphery on cam body top.When motor drives rocking bar and goods rack moved in a circle, rocking bar was subjected to action of cam, and rotate around bearing pin the rear end, and front end has been scratched on cam the time, and the indentation, there between cam falls.A water bath is laid in the outside of the indentation, there between each cam, when making rocking bar be in indentation, there, hangs biological sample on goods rack and is immersed in just in the water in the groove.The rotation of electric motor with stop, the water temperature in each water bath, the time that biological sample soaks in different water baths and the cycle index of soaking in turn control by single board computer.
The utility model is not structurally done any change to three different constant temperature water baths of original temperature, only require that they put by a graded, make the indentation, there of biological sample between cam on the goods rack, can be dipped into when falling in the water in the groove and get final product with goods rack; The extraction mechanism of being made up of cam body, electric motor, rocking bar and goods rack is operated automatically by computer program and is replaced manual operation to finish repeatedly circulation automatically, has alleviated the hand labour amount; The delay time that biological sample soaks in each water bath is by the computer program timing, and agreeing property is easy; Automatically the break-make of controlling electric heater by single board computer keeps the constant of water temperature in each water bath, and temperature control system is become simply.
Concrete structure of the present utility model is provided by following examples and accompanying drawing.
Fig. 1 is a sectional view of the present utility model;
Fig. 2 is a partial top view of the present utility model;
Fig. 3 is the sectional view of extraction mechanism, the rocking bar among the figure shown in the dotted line (7) on cam, the indentation, there of the rocking bar shown in the solid line (7) between cam;
Fig. 4 is the vertical view of cam, and the dash area among the figure is the indentation, there between the cam;
Fig. 5 is the stretch-out view of cam;
Fig. 6 soaks in each water bath in turn and the single board computer control flow chart of soak time about biological sample;
Fig. 7 is the thermostatic control skeleton diagram of water bath (11), and (19) among the figure are for giving the desired temperature of putting.
Describe structure of the present utility model and working condition in detail below in conjunction with accompanying drawing.
An embodiment called after PCR-90 type DNA cloning instrument of the present utility model, it comprises a single board computer (18), three constant temperature water bath (11), (12), (13) and cover extraction mechanism that have temperature sensor (16) that temperature is different.Electric heater (17) is arranged in the bottom of each water bath, three-arrive-place transmitter (15) is arranged in the outside of extraction mechanism, these equipment are installed on the block plate chassis (21), add aluminium alloy case (20).By the keyboard and the high brightness digital display screen (22) of this amplification positive tilted-putted single board computer of instrument (18), monitor the working condition of this amplification instrument.
Extraction mechanism is by cam body (8), electric motor (10), rocking bar (7) and goods rack (9) are formed, cylindrical by the cam body (8) that stainless steel is made, the identical passive type cam of three sizes of uniform distribution on the periphery on its top, electric motor (10) is in cam body (8), the two is linked together by web plate (4), electric motor (10) is subjected to the control of single board computer (18), the output shaft (3) of electric motor (10) drives main shaft (2), main shaft (2) is supported on the bearing (5), the rocking bar of being made by stainless steel (7) is driven by main shaft (2) that roller bearing (6) rolls along the cam gabarit on the cam body (8) when moving in a circle, rocking bar (7) is subjected to action of cam, rotate around bearing pin (1) rear end, front end and the goods rack (9) that connects together with front end with the variation of cam gabarit on, lower swing.
It is on the circumference of cylindrical at center that three constant temperature water baths (11), (12), (13) that temperature is different are distributed in cam body (8), three water baths respectively with cam body (3) on three cams between breach corresponding.When rocking bar (7) moves on cam, goods rack (9) carries biological sample (14) and is synchronized with the movement, when rocking bar (7) moves to indentation, there between the cam to level sensor (15) to signal that puts in place of single board computer (18) input, single board computer (18) is exported one immediately and is shut down order, electric motor (10) shuts down, goods rack (9) drops on the top of water bath (13) just, biological sample (14) just is immersed in the water bath (13), when soak time reaches the computer program set(ting)value of soaking time-delay in water bath (13), single board computer (18) makes electric motor (10) turn round once more, rocking bar (7) is by next cam jack-up, and goods rack (9) and biological sample (14) break away from water bath (13); When rocking bar (7) moves to indentation, there between the next protruding opinion, electric motor (10) stops once more, the top biological sample (14) that goods rack (9) drops on water bath (12) just just is immersed in when soak time reaches the computer program set(ting)value of soaking time-delay in water bath (12) in the water bath (12), single board computer (18) makes electric motor (10) turn round for the third time, rocking bar (7) is by next cam jack-up, goods rack (9) and biological sample (14) break away from water bath (12), when rocking bar (7) moves to indentation, there between the next cam, electric motor (10) stops for the third time, goods rack (9) drops on the top of water bath (11) just, biological sample just is immersed in the water bath (11), when soak time reaches the computer program set(ting)value of soaking time-delay in water bath (11), finish once circulation, the counter of single board computer (18) subtracts one, then enter circulation next time again, when rolling counters forward was zero, experiment finished.
Single board computer (18) is accepted the temperature value of temperature sensor in each water bath (16) input, and with computer program in each groove desired temperature relatively, when the water temperature in certain water bath departs from set(ting)value, single board computer (18) is controlled electric heater unit (17) energising or the outage in this groove, to keep the constant of water temperature in the groove.
Claims (3)
1, a kind of polymerase chain reaction DNA cloning instrument, it is formed by different three constant temperature water baths (11) of temperature, (12), (13) with extraction mechanism and control device that biological sample (14) soaks in each water bath in turn, it is characterized in that:
1. to be placed in cam body (8) be on the circumference of cylindrical at center water bath (11), (12), (13);
2. extraction mechanism is made up of cam body (8), electric motor (10), rocking bar (7) and goods rack (9), electric motor (10) is in cam body, both are linked together by web plate (4), the output shaft (3) of electric motor (10) drives main shaft (2), main shaft (2) is supported on the bearing (5), when rocking bar (7) was moved in a circle by main shaft (2) drive, roller bearing (6) rolled along the cam gabarit on the cam body (8), and the goods rack (9) of rocking bar (7) and front end thereof rotates around bearing pin (1).
2, amplification instrument according to claim 1 is characterized in that the identical passive type cam of three sizes of uniform distribution on the periphery on cam body (8) top.
3, amplification instrument according to claim 1 and 2 is characterized in that: water bath (11), (12), (13) respectively with cam body (8) on cam between indentation, there corresponding.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 90205278 CN2068968U (en) | 1990-04-27 | 1990-04-27 | Dna-expanding instrument for use in polymerase chain reaction |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 90205278 CN2068968U (en) | 1990-04-27 | 1990-04-27 | Dna-expanding instrument for use in polymerase chain reaction |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2068968U true CN2068968U (en) | 1991-01-09 |
Family
ID=4886075
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 90205278 Expired - Lifetime CN2068968U (en) | 1990-04-27 | 1990-04-27 | Dna-expanding instrument for use in polymerase chain reaction |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2068968U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108181946A (en) * | 2017-12-31 | 2018-06-19 | 江苏科泰检测技术服务有限公司 | Sensing chamber ice bath device and its application method |
| CN109797091A (en) * | 2019-01-17 | 2019-05-24 | 浙江大学 | A kind of rotary quickly dual temperature PCR amplification automatic control device and control method |
| CN109810891A (en) * | 2019-01-17 | 2019-05-28 | 浙江大学 | A kind of quick dual temperature PCR amplification automatic control device of rocker-type and control method |
| CN114350760A (en) * | 2022-01-12 | 2022-04-15 | 浙江正合谷生物科技有限公司 | PCR amplification instrument and method for nucleic acid on-site temperature-variable amplification |
-
1990
- 1990-04-27 CN CN 90205278 patent/CN2068968U/en not_active Expired - Lifetime
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108181946A (en) * | 2017-12-31 | 2018-06-19 | 江苏科泰检测技术服务有限公司 | Sensing chamber ice bath device and its application method |
| CN108181946B (en) * | 2017-12-31 | 2023-02-24 | 江苏科泰检测技术服务有限公司 | Ice bath device for detection room and use method thereof |
| CN109797091A (en) * | 2019-01-17 | 2019-05-24 | 浙江大学 | A kind of rotary quickly dual temperature PCR amplification automatic control device and control method |
| CN109810891A (en) * | 2019-01-17 | 2019-05-28 | 浙江大学 | A kind of quick dual temperature PCR amplification automatic control device of rocker-type and control method |
| CN114350760A (en) * | 2022-01-12 | 2022-04-15 | 浙江正合谷生物科技有限公司 | PCR amplification instrument and method for nucleic acid on-site temperature-variable amplification |
| CN114350760B (en) * | 2022-01-12 | 2024-04-12 | 浙江正合谷生物科技有限公司 | PCR amplification instrument and amplification method for on-site variable-temperature amplification of nucleic acid |
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| Date | Code | Title | Description |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CX01 | Expiry of patent term |