CN108174952A - A kind of folium cortex eucommiae polyphenol oxidase composite inhibitor and preparation method and application - Google Patents
A kind of folium cortex eucommiae polyphenol oxidase composite inhibitor and preparation method and application Download PDFInfo
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- CN108174952A CN108174952A CN201810073804.2A CN201810073804A CN108174952A CN 108174952 A CN108174952 A CN 108174952A CN 201810073804 A CN201810073804 A CN 201810073804A CN 108174952 A CN108174952 A CN 108174952A
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- cortex eucommiae
- folium cortex
- polyphenol oxidase
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/34—Tea substitutes, e.g. matè; Extracts or infusions thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/40—Colouring or decolouring of foods
- A23L5/41—Retaining or modifying natural colour by use of additives, e.g. optical brighteners
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Abstract
A kind of folium cortex eucommiae polyphenol oxidase composite inhibitor and preparation method and application, the folium cortex eucommiae polyphenol oxidase composite inhibitor is using water as solvent, containing 0.1 1.5g/L of vitamin C, 0.1 1.0g/L of L cysteines, 0.1 0.5g/L of kojic acid.Preparation method and application the invention also includes the folium cortex eucommiae polyphenol oxidase composite inhibitor.The compound polyphenol oxidase enzyme inhibitor of the present invention, each component synergy is good, and prevent-browning ability is strong, can control effectively to the brown stain in tea containing leaves of Eucommia ulmoides production process, and can protect the content of Chlorogenic Acid in Eucommia ulmoides Oliv. Leaves, flavones isoreactivity ingredient.
Description
Technical field
The present invention relates to a kind of folium cortex eucommiae polyphenol oxidase composite inhibitors and preparation method and application.
Background technology
Plant polyphenol has the effects that stronger removing free radical, anti-oxidant, anti-aging, radiation protection, antibacterial, is referred to as
" the 7th class nutrient " has a vast market prospect in food, cosmetics and pharmaceuticals industry.Polyphenol compound is through polyphenol
Oxidase catalyzed, oxidation forms the quinones substance and water of sepia, and quinones substance through non-enzymatic polymerization, forms dark brown object again
Matter eventually leads to the generation of brown stain.
Cortex Eucommiae is one of rare tonic Chinese herbal medicine in China, has effects that three drops, a six anti-increasing etc. is many, folium cortex eucommiae is China
The medicinal and edible plant that Ministry of Public Health's examination ＆ approval pass through, it contains a variety of active ingredients, the phenylpropyl alcohol such as flavonoids, based on chlorogenic acid
Plain class, lignanoids, iridoid etc..Wherein flavones and chlorogenic acid can be classified as plant polyphenol again from its structure
Class compound, content reaches 4% or so in folium cortex eucommiae.The manufacture craft of usual Eucommia Tea includes withering, finishing, rubbing, drying
Do and etc., the Eucommia Tea made is black, is primarily due to during rubbing, cell is destroyed, and folium cortex eucommiae is more
Oxygen is met in the exposure of the compounds such as phenol, high temperature easily generates brown stain.The hair of folium cortex eucommiae brown stain during production and processing is into Eucommia Tea
The variation in addition to causing appearance luster is given birth to, will also result in the variation of mouthfeel and the loss of nutriment.
Invention content
The technical problem to be solved by the invention is to provide a kind of brown in folium cortex eucommiae process of manufacture for controlling
Become, improve in folium cortex eucommiae the folium cortex eucommiae polyphenol oxidase composite inhibitor of the content of index ingredient chlorogenic acid, flavones etc. and its
Preparation method and application.
The technical solution adopted by the present invention to solve the technical problems is：
The folium cortex eucommiae polyphenol oxidase composite inhibitor of the present invention, using water as solvent, 0.1-1.5g/L containing vitamin C, L-
Cysteine 0.1-1.0g/L, kojic acid 0.1-0.5g/L.
Further, the water is distilled water or deionized water.
Further, the preferred 0.5-1.0g/L of the vitamin C.
Further, the preferred 0.3-0.7g/L of the L-cysteine.
Further, the preferred 0.2-0.4g/L of the kojic acid.
The preparation method of the folium cortex eucommiae polyphenol oxidase composite inhibitor of the present invention is added respectively by predetermined ratio into water
Enter vitamin C, L-cysteine, kojic acid, be uniformly mixed, obtain using water as solvent, 0.1-1.5g/L containing vitamin C, L-
The folium cortex eucommiae polyphenol oxidase composite inhibitor of cysteine 0.1-1.0g/L, kojic acid 0.1-0.5g/L.
Brown stain of the folium cortex eucommiae polyphenol oxidase composite inhibitor of the present invention in folium cortex eucommiae process of manufacture is controlled,
Improve folium cortex eucommiae in index ingredient chlorogenic acid, flavones content application.
Fresh folium cortex eucommiae is immersed in 1-3h in prepared folium cortex eucommiae polyphenol oxidase composite inhibitor, folium cortex eucommiae with
The mass ratio of polyphenol oxidase composite inhibitor is 1:8, composite inhibitor contains vitamin C (0.1-1.5g/ using water as solvent
L), L-cysteine (0.1-1.0g/L), kojic acid (0.1-0.5g/L), drain to water content 10-25wt%, microwave de-enzyming, cold
But, it rubs, dries, folium cortex eucommiae can keep the colour-fast discoloration of green, and Eucommia Tea Content of Chlorogenic Acid, flavones, total lignan etc. at this time
Component content is the 1.15 of the Eucommia Tea of produced in conventional processes, 1.2,1.4 times.
Microwave de-enzyming has the characteristics that heating is rapid, temperature is uniform, the thermal efficiency is high, fixation time is short and water-removing is uniform,
It is more advantageous to the reservation of active constituent in Eucommia Tea.Microwave de-enzyming power 6KW, rotating speed 300r/min.
The temperature of drying is 70-110 DEG C, is dried to water content as 2-5wt%.
If folium cortex eucommiae is without any processing, directly processes, rubbing step meeting blackening, Eucommia Tea Content of Chlorogenic Acid, Huang
Ketone, lignanoids, iridoids isoreactivity component content reduce.If folium cortex eucommiae is handled without composite inhibitor, directly wither
More than 10h, water content are down to 20%, and through water-removing, cooling is rubbed, and Eucommia Tea can keep green in the short time, but the later stage stores
Yellow green, yellow can be slowly become, influences product appearance, and Eucommia Tea Content of Chlorogenic Acid, flavones, lignanoids, iridoids
Isoreactivity component content can also reduce.
Research practice shows the compound polyphenol oxidase enzyme inhibitor of the present invention, and each component synergy is good, prevent-browning energy
Power is strong, can control effectively to the brown stain in tea containing leaves of Eucommia ulmoides production process, and can protect Chlorogenic Acid in Eucommia ulmoides Oliv. Leaves, flavones
The content of isoreactivity ingredient.
Specific embodiment
The invention will be further described with reference to embodiments.
Embodiment 1
The folium cortex eucommiae polyphenol oxidase composite inhibitor of the present invention, using distilled water as solvent, 0.5g/L containing vitamin C, L-
Cysteine 0.3g/L, kojic acid 0.3g/L.
Preparation method, be separately added by predetermined ratio into distilled water 100L vitamin C 50g, L-cysteine 30g,
Kojic acid 30g is uniformly mixed, obtains using water as solvent, 0.5g/L containing vitamin C, L-cysteine 0.3g/L, kojic acid
The folium cortex eucommiae polyphenol oxidase composite inhibitor of 0.3g/L.
Brown stain of the folium cortex eucommiae polyphenol oxidase composite inhibitor of the present invention in folium cortex eucommiae process of manufacture is controlled,
Improve folium cortex eucommiae in index ingredient chlorogenic acid, flavones content application.
Fresh folium cortex eucommiae is immersed in 2h in prepared folium cortex eucommiae polyphenol oxidase composite inhibitor, folium cortex eucommiae with it is more
The mass ratio of phenol oxidase composite inhibitor is 1:8, composite inhibitor contains vitamin C (0.5g/L), L- using water as solvent
Cysteine (0.3g/L), kojic acid (0.3g/L) are drained to water content 20wt%, microwave de-enzyming, and cooling is rubbed, and is dried, this
When folium cortex eucommiae can keep the colour-fast discoloration of green, and the component contents such as Eucommia Tea Content of Chlorogenic Acid, flavones, total lignan are tradition
1.15,1.2,1.4 times of the Eucommia Tea of method production.
Microwave de-enzyming has the characteristics that heating is rapid, temperature is uniform, the thermal efficiency is high, fixation time is short and water-removing is uniform,
It is more advantageous to the reservation of active constituent in Eucommia Tea.Microwave de-enzyming power 6KW, rotating speed 300r/min.
The temperature of drying is 90-100 DEG C, is dried to water content as 3wt%.
If folium cortex eucommiae is without any processing, directly processes, rubbing step meeting blackening, Eucommia Tea Content of Chlorogenic Acid, Huang
Ketone, lignanoids, iridoids isoreactivity component content reduce.If folium cortex eucommiae is handled without composite inhibitor, directly wither
More than 10h, water content are down to 20%, and through water-removing, cooling is rubbed, and Eucommia Tea can keep green in the short time, but the later stage stores
Yellow green, yellow can be slowly become, influences product appearance, and Eucommia Tea Content of Chlorogenic Acid, flavones, lignanoids, iridoids
Isoreactivity component content can also reduce.
Research practice shows the compound polyphenol oxidase enzyme inhibitor of the present invention, and each component synergy is good, prevent-browning energy
Power is strong, can control effectively to the brown stain in tea containing leaves of Eucommia ulmoides production process, and can protect Chlorogenic Acid in Eucommia ulmoides Oliv. Leaves, flavones
The content of isoreactivity ingredient.
Embodiment 2
The folium cortex eucommiae polyphenol oxidase composite inhibitor of the present invention, using distilled water as solvent, 0.8g/L containing vitamin C, L-
Cysteine 0.5g/L, kojic acid 0.4g/L.
Preparation method, be separately added by predetermined ratio into distilled water 100L vitamin C 80g, L-cysteine 50g,
Kojic acid 40g is uniformly mixed, obtains using water as solvent, 0.8g/L containing vitamin C, L-cysteine 0.5g/L, kojic acid
The folium cortex eucommiae polyphenol oxidase composite inhibitor of 0.4g/L.
Brown stain of the folium cortex eucommiae polyphenol oxidase composite inhibitor of the present invention in folium cortex eucommiae process of manufacture is controlled,
Improve folium cortex eucommiae in index ingredient chlorogenic acid, flavones content application.
Fresh folium cortex eucommiae is immersed in 3h in prepared folium cortex eucommiae polyphenol oxidase composite inhibitor, folium cortex eucommiae with it is more
The mass ratio of phenol oxidase composite inhibitor is 1:8, composite inhibitor contains vitamin C (0.8g/L), L- using water as solvent
Cysteine (0.5g/L), kojic acid (0.4g/L) are drained to water content 20wt%, microwave de-enzyming, and cooling is rubbed, and is dried, this
When folium cortex eucommiae can keep the colour-fast discoloration of green, and the component contents such as Eucommia Tea Content of Chlorogenic Acid, flavones, total lignan are tradition
1.15,1.2,1.4 times of the Eucommia Tea of method production.
Microwave de-enzyming has the characteristics that heating is rapid, temperature is uniform, the thermal efficiency is high, fixation time is short and water-removing is uniform,
It is more advantageous to the reservation of active constituent in Eucommia Tea.Microwave de-enzyming power 6KW, rotating speed 300r/min.
The temperature of drying is 95-100 DEG C, is dried to water content as 4wt%.
If folium cortex eucommiae is without any processing, directly processes, rubbing step meeting blackening, Eucommia Tea Content of Chlorogenic Acid, Huang
Ketone, lignanoids, iridoids isoreactivity component content reduce.If folium cortex eucommiae is handled without composite inhibitor, directly wither
More than 10h, water content are down to 20%, and through water-removing, cooling is rubbed, and Eucommia Tea can keep green in the short time, but the later stage stores
Yellow green, yellow can be slowly become, influences product appearance, and Eucommia Tea Content of Chlorogenic Acid, flavones, lignanoids, iridoids
Isoreactivity component content can also reduce.
Research practice shows the compound polyphenol oxidase enzyme inhibitor of the present invention, and each component synergy is good, prevent-browning energy
Power is strong, can control effectively to the brown stain in tea containing leaves of Eucommia ulmoides production process, and can protect Chlorogenic Acid in Eucommia ulmoides Oliv. Leaves, flavones
The content of isoreactivity ingredient.
Embodiment 3
The folium cortex eucommiae polyphenol oxidase composite inhibitor of the present invention, using distilled water as solvent, 1.0g/L containing vitamin C, L-
Cysteine 0.7g/L, kojic acid 0.4g/L.
Preparation method, be separately added by predetermined ratio into distilled water 100L vitamin C 100g, L-cysteine 70g,
Kojic acid 40g is uniformly mixed, obtains using water as solvent, 1.0g/L containing vitamin C, L-cysteine 0.7g/L, kojic acid
The folium cortex eucommiae polyphenol oxidase composite inhibitor of 0.4g/L.
Brown stain of the folium cortex eucommiae polyphenol oxidase composite inhibitor of the present invention in folium cortex eucommiae process of manufacture is controlled,
Improve folium cortex eucommiae in index ingredient chlorogenic acid, flavones content application.
Fresh folium cortex eucommiae is immersed in 2h in prepared folium cortex eucommiae polyphenol oxidase composite inhibitor, folium cortex eucommiae with it is more
The mass ratio of phenol oxidase composite inhibitor is 1:8, composite inhibitor contains vitamin C (1.0g/L), L- using water as solvent
Cysteine (0.7g/L), kojic acid (0.4g/L) are drained to water content 25wt%, microwave de-enzyming, and cooling is rubbed, and is dried, this
When folium cortex eucommiae can keep the colour-fast discoloration of green, and the component contents such as Eucommia Tea Content of Chlorogenic Acid, flavones, total lignan are tradition
1.15,1.2,1.4 times of the Eucommia Tea of method production.
Microwave de-enzyming has the characteristics that heating is rapid, temperature is uniform, the thermal efficiency is high, fixation time is short and water-removing is uniform,
It is more advantageous to the reservation of active constituent in Eucommia Tea.Microwave de-enzyming power 6KW, rotating speed 300r/min.
The temperature of drying is 90-100 DEG C, is dried to water content as 4wt%.
If folium cortex eucommiae is without any processing, directly processes, rubbing step meeting blackening, Eucommia Tea Content of Chlorogenic Acid, Huang
Ketone, lignanoids, iridoids isoreactivity component content reduce.If folium cortex eucommiae is handled without composite inhibitor, directly wither
More than 10h, water content are down to 20%, and through water-removing, cooling is rubbed, and Eucommia Tea can keep green in the short time, but the later stage stores
Yellow green, yellow can be slowly become, influences product appearance, and Eucommia Tea Content of Chlorogenic Acid, flavones, lignanoids, iridoids
Isoreactivity component content can also reduce.
Research practice shows the compound polyphenol oxidase enzyme inhibitor of the present invention, and each component synergy is good, prevent-browning energy
Power is strong, can control effectively to the brown stain in tea containing leaves of Eucommia ulmoides production process, and can protect Chlorogenic Acid in Eucommia ulmoides Oliv. Leaves, flavones
The content of isoreactivity ingredient.
Claims (9)
1. a kind of folium cortex eucommiae polyphenol oxidase composite inhibitor, it is characterised in that：Using water as solvent, 0.1- containing vitamin C
1.5g/L, L-cysteine 0.1-1.0g/L, kojic acid 0.1-0.5g/L.
2. folium cortex eucommiae polyphenol oxidase composite inhibitor according to claim 1, it is characterised in that：The water is distilled water
Or deionized water.
3. folium cortex eucommiae polyphenol oxidase composite inhibitor according to claim 1 or 2, it is characterised in that：The vitamin C
For 0.5-1.0g/L.
4. folium cortex eucommiae polyphenol oxidase composite inhibitor according to claim 1 or 2, it is characterised in that：Half Guangs of L-
Propylhomoserin is 0.3-0.7g/L.
5. folium cortex eucommiae polyphenol oxidase composite inhibitor according to claim 3, it is characterised in that：The L-cysteine
For 0.3-0.7g/L.
6. folium cortex eucommiae polyphenol oxidase composite inhibitor according to claim 1 or 2, it is characterised in that：The kojic acid is
0.2-0.4g/L。
7. folium cortex eucommiae polyphenol oxidase composite inhibitor according to claim 5, it is characterised in that：The kojic acid is 0.2-
0.4g/L。
8. a kind of preparation method of folium cortex eucommiae polyphenol oxidase composite inhibitor as described in claim 1, it is characterised in that：It presses
Predetermined ratio is separately added into vitamin C, L-cysteine, kojic acid into water, is uniformly mixed, obtains using water as solvent, containing dimension
The compound suppression of folium cortex eucommiae polyphenol oxidase of raw element C 0.1-1.5g/L, L-cysteine 0.1-1.0g/L, kojic acid 0.1-0.5g/L
Preparation.
9. a kind of folium cortex eucommiae polyphenol oxidase composite inhibitor as described in claim 1 is in control folium cortex eucommiae process of manufacture
In brown stain, improve folium cortex eucommiae in index ingredient chlorogenic acid, flavones content application.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109349473A (en) * | 2018-11-23 | 2019-02-19 | 盐城工学院 | A kind of Brazilian certain kind of berries fruit juice and preparation method thereof with fat-reducing effect |
| CN109524064A (en) * | 2018-11-12 | 2019-03-26 | 云南省烟草农业科学研究院 | A kind of virtual screening method of polyphenol oxidase enzyme inhibitor |
| CN110923251A (en) * | 2019-12-19 | 2020-03-27 | 中国烟草总公司郑州烟草研究院 | Tobacco polyphenol oxidase NtPPO4 and application thereof |
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| CN102040520A (en) * | 2010-11-15 | 2011-05-04 | 浙江工业大学 | Method for separating and purifying chlorogenic acid from eucommia bark leaves |
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| CN102040520A (en) * | 2010-11-15 | 2011-05-04 | 浙江工业大学 | Method for separating and purifying chlorogenic acid from eucommia bark leaves |
| CN106932508A (en) * | 2016-11-23 | 2017-07-07 | 河北中医学院 | A kind of preparation method and application of honeysuckle polyphenol oxidase composite inhibitor |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109524064A (en) * | 2018-11-12 | 2019-03-26 | 云南省烟草农业科学研究院 | A kind of virtual screening method of polyphenol oxidase enzyme inhibitor |
| CN109524064B (en) * | 2018-11-12 | 2020-10-20 | 云南省烟草农业科学研究院 | Virtual screening method of polyphenol oxidase inhibitor |
| CN109349473A (en) * | 2018-11-23 | 2019-02-19 | 盐城工学院 | A kind of Brazilian certain kind of berries fruit juice and preparation method thereof with fat-reducing effect |
| CN110923251A (en) * | 2019-12-19 | 2020-03-27 | 中国烟草总公司郑州烟草研究院 | Tobacco polyphenol oxidase NtPPO4 and application thereof |
| CN110923251B (en) * | 2019-12-19 | 2022-07-08 | 中国烟草总公司郑州烟草研究院 | Tobacco polyphenol oxidase NtPPO4 and application thereof |
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Application publication date: 20180619 |