CN108142379A - For differentiating and observing the feeder of bacterium feeding habits and the small mite class of phytophagous - Google Patents
For differentiating and observing the feeder of bacterium feeding habits and the small mite class of phytophagous Download PDFInfo
- Publication number
- CN108142379A CN108142379A CN201711319666.3A CN201711319666A CN108142379A CN 108142379 A CN108142379 A CN 108142379A CN 201711319666 A CN201711319666 A CN 201711319666A CN 108142379 A CN108142379 A CN 108142379A
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- room floor
- feeder
- bacterium
- circular hole
- cover plate
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- 241000894006 Bacteria Species 0.000 title claims abstract description 51
- 238000000034 method Methods 0.000 claims abstract description 12
- 239000012780 transparent material Substances 0.000 claims abstract description 4
- 238000002955 isolation Methods 0.000 claims description 17
- 239000011521 glass Substances 0.000 claims description 12
- 238000009395 breeding Methods 0.000 claims description 7
- 230000001488 breeding effect Effects 0.000 claims description 7
- 239000001963 growth medium Substances 0.000 claims description 5
- 241000233866 Fungi Species 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 4
- 230000004083 survival effect Effects 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 235000013305 food Nutrition 0.000 description 5
- 239000005357 flat glass Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000005553 drilling Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 238000012271 agricultural production Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000009400 out breeding Methods 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000011800 void material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention belongs to agriculture fields, it proposes a kind of for differentiating and observe bacterium feeding habits and the feeder of the small mite class of phytophagous, the feeder includes raising room floor and cover plate, the raising room floor is made of transparent material, the perforation circular hole of a diameter of 15 18mm is provided in raising room floor or has bottom circular hole, cover plate and receptacle stacking are put, and are fixed with clip.The present invention also proposes a kind of method differentiated and observe bacterium feeding habits and the small mite class of phytophagous.Feeder proposed by the present invention is simple for production, is easy to observe, and can effectively prevent the escape of mite, and feeding environment is also relatively uniform.
Description
Technical field
The invention belongs to agriculture fields, and in particular to a kind of device for differentiating and observing for small mite class feeding habits.
Background technology
Some microminiature mite class feeding habits are complicated, you can feeding plant also can use mould feeder, in order to observe with its clear and definite feeding habits,
It needs to develop special device, convenient for observing its unique biological habit.Such as some types of certain tarsonemids have become
The important evil mite of agricultural production, but since its individual is very small, it is kind to creep, there is skototaxis, feeding habits are complicated, are not easy to observe.
Lack necessary observation device and means at present, so the mite class biological habit research to this type is less, it is raw to agricultural
The harm that such mite is solved in production brings certain difficulty.
Common mite class method for breeding in experiment, substantially on the raising of excised leaf opening, often result in the big of mite
Amount escape；And to verify whether it relies solely on food bacterium survival, it is even more very difficult.The present invention preferably solves above-mentioned difficulty
Topic successfully completes the raising of tarsonemid and observation experiment, also provides necessary means for some small mite class Biology Observations.
Invention content
For shortcoming existing for this field, the purpose of the present invention is to propose to one kind for differentiate and observe bacterium feeding habits and
The feeder of the small mite class of phytophagous.
It is another object of the present invention to propose a kind of method differentiated and observe bacterium feeding habits and the small mite class of phytophagous.
Realize the object of the invention technical solution be：
It is a kind of for differentiating and observing bacterium feeding habits and the feeder of the small mite class of phytophagous, the feeder includes receptacle
Layer and cover plate, the raising room floor are made of transparent material, raise and having bottom circular hole or passing through for a diameter of 15-18mm is provided in room floor
Logical circular hole, cover plate and receptacle stacking are put, and are fixed with clip.
Wherein, the feeder includes bacterium culture room floor, isolation sieve, raises room floor and cover plate, in the bacterium culture room floor
Be provided with a diameter of 15-18mm has bottom circular hole, and the isolation sieve is to paste gauze on perforated panel to be made (gauze is upper), described
The perforation circular hole of a diameter of 15-18mm is provided in raising room floor；The bacterium culture room floor, isolation sieve, raising room floor and cover plate are suitable
Sequence stacks, and keeps the middle overlapping of axles of two circular holes of raising room floor and bacterium culture room floor, is fixed with clip.
Bacterium culture room floor, isolation sieve, raising room floor and cover plate four are partly superimposed together from bottom to up, edge alignment,
I.e. receptacle circular hole is aligned, then four parts are fixed together with clip with culturing room's band bottom circular hole, that is, forms the raising of bacterium feeding habits
Device.Material used above is not limited only to organic glass and glass, other are easily punched, the transparent material easily observed.
Preferably, the raising room floor is made of organic glass, and the length × width × height for raising room floor is (70-80) × (20-
30)×(4-6)mm；The cover plate is transparent glass sheet, and the length × width × height of cover plate is (70-80) × (20-30) × 1mm.
Isolation sieve is pasted warp layer net by the plastic plate of hollow out and is formed, and gauze mesh is determined according to the size of mite, is ensured
Each worm state of mite cannot pass through gauze.The plastic plate of hollow out plays the role of support to gauze, prevents gauze in use
Recess avoids gauze from touching culture medium, and guarantee mite can only be food to pass through the hypha,hyphae of gauze in raising room floor, simultaneously
By gauze and the minim gap of the closed formation of both ends material, air needed for existence is provided for mite.The void region of plastic plate
It is less than the diameter of raising chamber and receptacle.
Specifically, the perforated panel is made of plastic plate, 3-4 hole is provided on perforated panel, the area in hole is 20-30mm2,
The length × width × height of perforated panel is (20-30) × (20-30) × (0.2-1) mm, the center of opening area and raising on perforated panel
The middle overlapping of axles of perforation circular hole in room floor.
Wherein, bacterium culture room floor is made of organic glass, the length × width × height of bacterium culture room floor for (70-80) ×
(20-30)×(4-6)mm.The thickness for having the bottom of bottom circular hole in bacterium culturing room is 0.5-1mm.
A kind of method differentiated and observe bacterium feeding habits and the small mite class of phytophagous：It is raised using phytophagous feeder or bacterium feeding habits
Support device.
The phytophagous feeder includes raising room floor and cover plate, raises and has been provided with bottom circular hole in room floor；The bacterium feeding habits
Feeder includes bacterium culture room floor, isolation sieve, raising room floor and cover plate, raises and is provided with perforation circular hole in room floor.
During using the phytophagous feeder, plant leaf blade is cut into the disk of diameter 10-15mm, is placed on the receptacle
The circular hole bottom of layer, then at least 1 pair of the female male imago of small mite class is put into the circular hole for having bottom raising room floor, cover plate is covered, is raised
Support the existence of observation mite and breeding situation.
Using bacterium feeding habits feeder, fungi culture medium is put into the circular hole of bacterium culture room floor, inoculated fungi is consolidated with clip
The fixed bacterium culture room floor, isolation sieve, bottomless raising room floor and cover plate, when hypha,hyphae passes through gauze, in raising room floor
At least 1 pair of the female male imago of small mite class is put into circular hole, covers cover plate, the existence of breeding observing mite and breeding situation.
The beneficial effects of the present invention are：
Feeder proposed by the present invention is simple for production, is easy to observe, and can effectively prevent the escape of mite, and feeding environment is also opposite
It is unified.
Feeder of the present invention raises tiny organism such as tarsonemid and its observation experiment, has the following advantages that：
Phytophagous feeder, easily operated, storage is convenient.Feeder is transparent, and the size of receptacle just exists
Under the visual field of anatomical lens, it is extremely easy to experimental observation.Receptacle is substantially what is closed, but in unlike material engaging portion due to material
The coefficient of expansion it is different and generate minim gap, can be that raising object provide air, and mite escape can effectively be prevented to be missing, mite institute
The environment at place is relatively uniform, greatly improves the accuracy of experimental data.
Bacterium feeding habits feeder, not only has many advantages, such as the easy observation of phytophagous feeder, outside anti-escape, it is ensured that raising
Interior can be used as food in addition to hypha,hyphae, no other things, convenient for identifying whether raised mite class has bacterium feeding habits.Gauze
Engaging portion can provide existence air for the tiny organism in bacterium feeding habits feeder, ensure its survival.
Description of the drawings
Fig. 1 phytophagous feeder photos,
Fig. 2：The photo of bacterium feeding habits feeder depression angle.
The photo of the side view angle of Fig. 3 bacterium feeding habits feeders
Fig. 4 is bottomless raising room floor photo
Fig. 5 is isolation sieve photo, and wherein left figure is gauze in upper full face, and right figure is reverse side photo.
Fig. 6 is bacterium feeding habits feeder structure diagram.
In figure, 1 is cover plate, and 2 be raising room floor, and 3 cultivate room floor for isolation sieve, 4 for bacterium.
Specific embodiment
The present invention is now illustrated, but be not limited to the scope of the present invention with following embodiment.The hand used in embodiment
Section, unless otherwise instructed, using the means of this field routine.
The preparation and application of 1 bacterium feeding habits feeder of embodiment
The glass slide that length × width x thickness is 76mm × 25mm × 1mm is taken as cover plate.
The sheet glass that length × width x thickness is taken to be 76mm × 25mm × 5mm, a diameter is made a call to drilling machine in sheet glass centre
Circular hole penetrating 16mm forms bottomless raising room floor (photo is shown in Fig. 4).
The pad of 450 mesh gauzes and 0.5mm thickness is write into plate and is cut into 25mm × 25mm sizes, plate is write in above-mentioned pad with puncher
The hole of 4 diameter 6mm is made a call in center, and into Openworks shape, the two alignment overlay edge is glued as isolation screen layers (referring to Fig. 5).
It is 76mm × 25mm × 5mm sizes by organic glass cut growth × width x thickness of 5mm thickness, with drilling machine organic
Sheet glass centre makes a call to an a diameter of 16mm, and the band bottom circular hole of deep 4mm can be used as culture room floor 4.
Bacterium culture room floor 4 (bacterium culturing room is most lower), isolation sieve 3, raising room floor 2 and 1 composite structure of cover plate referring to Fig. 6,
The photo of combination is shown in Fig. 2 and Fig. 3.
Application process：
The PDA culture medium of 0.1ml is put into culture room floor hole, Apple Mould Core disease fungus is connect on culture medium, so
It is aligned in order with screen layers, raising room floor and cover plate is isolated again afterwards, fixation is clamped with binder clip, treat that hypha,hyphae passes through isolation to sieve
Gauze when, you can in receptacle access tarsonemid male and female it is right into mite 1, carry out breeding observing.For prevent unclamp clip into
When row observation or other operations, each section easily misplaces, and in advance can simply be bonded at each section edge with glue.The method
The female of raising survives 20 days into mite, lays eggs 72 altogether.Illustrate that tarsonemid has food bacterium property, independent feeding hypha,hyphae can be with
Meet the growth and breeding of tarsonemid.The method causes in receptacle in addition to hypha,hyphae, no other things can be used as food, be convenient for
Whether the raised mite class of identification has bacterium feeding habits；Feeder is transparent, and the size of receptacle is just in anatomical lens
Under the visual field, it is extremely easy to experimental observation.
The preparation and application of 2. phytophagous receptacle of embodiment
The glass slide that length × width x thickness is 76mm × 25mm × 1mm is taken as cover plate.
It is 76mm × 25mm × 5mm sizes by organic glass cut growth × width x thickness of 5mm thickness, with drilling machine organic
Sheet glass centre makes a call to an a diameter of 16mm, the band bottom circular hole of deep 4mm, as raising room floor with the end.Raise room floor and cover plate
Combined photograph is shown in Fig. 1.
Application process：
Apple Leaves with card punch are cut into the disk of a diameter of 12mm, are put into the hole of bottom receptacle, then choose into instep
Line mite male and female are right into mite 5, cover cover plate and clamp.In addition using conventional water pond method, it is right into mite 5 that tarsonemid male and female are raised.24h
After observe, as a result show 5 pairs of tarsonemids in phytophagous feeder still in feeder, and 5 pairs of instep lines of conventional water pond raising
Mite has 1 female to flee from blade into mite.It can be seen that the phytophagous feeder of the present invention can use, with conventional method ratio
Compared with effectively preventing the escape of tarsonemid.
Above embodiment be only the preferred embodiment of the present invention is described, not to the scope of the present invention into
Row limits, and under the premise of design spirit of the present invention is not departed from, this field ordinary skill technical staff is to the technical side of the present invention
The all variations and modifications that case is made should all be fallen into the protection domain that claims of the present invention determines.
Claims (8)
- It is 1. a kind of for differentiating and observe bacterium feeding habits and the feeder of the small mite class of phytophagous, which is characterized in that the feeder Including raising room floor and cover plate, the raising room floor is made of transparent material, raises and is provided with passing through for a diameter of 15-18mm in room floor Logical circular hole has bottom circular hole, and cover plate and receptacle stacking are put, and are fixed with clip.
- 2. feeder according to claim 1, which is characterized in that the feeder includes bacterium culture room floor, isolation sieve, raises Room floor and cover plate are supported, a diameter of 15-18mm is provided in the bacterium culture room floor has bottom circular hole, and the isolation sieve is in perforated panel Upper stickup gauze is made；The perforation circular hole of a diameter of 15-18mm, the bacterium culture room floor, isolation are provided in the raising room floor Sieve, raising room floor and cover plate sequentially stack from bottom to top, and keep the axis of two circular holes of raising room floor and bacterium culture room floor It overlaps, is fixed with clip.
- 3. feeder according to claim 1 or 2, which is characterized in that the raising room floor is made of organic glass, raises The length × width × height of room floor is (70-80) × (20-30) × (4-6) mm；The cover plate is transparent glass sheet, the length of cover plate × wide × a height of (70-80) × (20-30) × 1mm.
- 4. feeder according to claim 2, which is characterized in that the perforated panel is made of plastic plate, is opened on perforated panel There is 3-4 hole, the area in hole is 20-30mm2, the length × width × height of perforated panel is (20-30) × (20-30) × (0.2-1) mm, The center of opening area and the middle overlapping of axles of the perforation circular hole in raising room floor on perforated panel.
- 5. feeder according to claim 2, which is characterized in that the bacterium culture room floor is made of organic glass, bacterium training The length × width × height for supporting room floor is (70-80) × (20-30) × (4-6) mm；There is the thickness at the bottom of bottom circular hole in bacterium culture room floor For 0.5-1mm.
- A kind of 6. method differentiated and observe bacterium feeding habits and the small mite class of phytophagous, which is characterized in that use phytophagous feeder Or bacterium feeding habits feeder differentiates and observes；The phytophagous feeder includes raising room floor and cover plate, raises and has been provided with bottom circular hole in room floor；The bacterium feeding habits raising Device includes bacterium culture room floor, isolation sieve, raising room floor and cover plate, raises and is provided with perforation circular hole in room floor.
- 7. according to the method described in claim 6, it is characterized in that, it is using the mode of phytophagous feeder：By plant leaf blade It cleans up, is cut into the disk of diameter 10-15mm, be placed on the circular hole bottom of the raising room floor, then the circular hole in raising room floor At least 1 pair of the female male imago of small mite class is inside put into, covers cover plate, the survival and reproduction situation of breeding observing mite.
- 8. according to the method described in claim 6, it is characterized in that, it is using the mode of bacterium feeding habits feeder：In bacterium culturing room Be put into culture medium in the circular hole of layer, inoculated fungi, with clip fix the bacterium culture room floor, isolation sieve, bottomless raising room floor and When hypha,hyphae passes through gauze, at least 1 pair of the female male imago of small mite class is put into the circular hole of bottomless raising room floor for cover plate, Cover cover plate, the survival and reproduction situation of breeding observing mite.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711319666.3A CN108142379B (en) | 2017-12-12 | 2017-12-12 | Feeder for identifying and observing edible fungus and phytophagous tiny mites |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711319666.3A CN108142379B (en) | 2017-12-12 | 2017-12-12 | Feeder for identifying and observing edible fungus and phytophagous tiny mites |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108142379A true CN108142379A (en) | 2018-06-12 |
| CN108142379B CN108142379B (en) | 2020-03-20 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711319666.3A Active CN108142379B (en) | 2017-12-12 | 2017-12-12 | Feeder for identifying and observing edible fungus and phytophagous tiny mites |
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| Country | Link |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113163741A (en) * | 2018-10-05 | 2021-07-23 | 百澳思集团公众有限公司 | Mite composition and method for feeding mites |
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| Publication number | Publication date |
|---|---|
| CN108142379B (en) | 2020-03-20 |
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