CN108138128A - 用于悬浮细胞的小规模养殖方法 - Google Patents

用于悬浮细胞的小规模养殖方法 Download PDF

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Publication number
CN108138128A
CN108138128A CN201680030077.0A CN201680030077A CN108138128A CN 108138128 A CN108138128 A CN 108138128A CN 201680030077 A CN201680030077 A CN 201680030077A CN 108138128 A CN108138128 A CN 108138128A
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culture
cells
scale
cell
small
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English (en)
Chinese (zh)
Inventor
S·戈勒兹
R·施塔恩
S·克雷耶
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Glycotope GmbH
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Glycotope GmbH
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Application filed by Glycotope GmbH filed Critical Glycotope GmbH
Publication of CN108138128A publication Critical patent/CN108138128A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/04—Immortalised cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201680030077.0A 2015-05-29 2016-05-25 用于悬浮细胞的小规模养殖方法 Pending CN108138128A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
LU92727 2015-05-29
LU92727 2015-05-29
EP16151811 2016-01-19
EP16151811.3 2016-01-19
PCT/EP2016/061763 WO2016193083A1 (en) 2015-05-29 2016-05-25 Small scale cultivation method for suspension cells

Publications (1)

Publication Number Publication Date
CN108138128A true CN108138128A (zh) 2018-06-08

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CN201680030077.0A Pending CN108138128A (zh) 2015-05-29 2016-05-25 用于悬浮细胞的小规模养殖方法

Country Status (10)

Country Link
US (1) US20180135091A1 (https=)
EP (1) EP3303557B1 (https=)
JP (1) JP6737813B2 (https=)
KR (1) KR20180010200A (https=)
CN (1) CN108138128A (https=)
AU (1) AU2016272566A1 (https=)
BR (1) BR112017023624A2 (https=)
CA (1) CA2985435A1 (https=)
RU (1) RU2730656C2 (https=)
WO (1) WO2016193083A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111139216A (zh) * 2018-11-01 2020-05-12 上海药明生物技术有限公司 高通量、自动化的细胞连续培养方法及其应用
CN114599782A (zh) * 2019-07-01 2022-06-07 科学生物技术公司 用于在多并联生物反应器中培养粘附细胞的方法

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3303557B1 (en) 2015-05-29 2021-05-05 Glycotope GmbH Small scale cultivation method for suspension cells
EP3472338B1 (en) 2016-06-20 2024-05-22 Octapharma AG Means and methods for modifying multiple alleles
WO2018062358A1 (ja) * 2016-09-29 2018-04-05 Ｃｏａｉｄｏ株式会社 災害緊急用地域一斉架電システム
JP7317466B2 (ja) 2017-12-12 2023-07-31 株式会社日立製作所 細胞株及び培養条件のスクリーニング方法、及びその装置
WO2020201296A1 (en) * 2019-04-01 2020-10-08 The Automation Partnership (Cambridge) Ltd. Operation process for a cell cultivation system
KR20220006066A (ko) * 2019-05-08 2022-01-14 지머젠 인코포레이티드 대규모의 성능 예측을 개선하기 위해 소규모 미생물에 대한 실험 및 플레이트 모델을 디자인하기 위한 매개변수 축소
CN118064651B (zh) * 2024-03-11 2025-03-11 上海迈邦生物科技有限公司 一种细胞灌流培养体系维持稳态的方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0599651A2 (en) * 1992-11-25 1994-06-01 National Research Council Of Canada Bioreactor for the perfusion culture of cells
CN101490239A (zh) * 2006-07-14 2009-07-22 帝斯曼知识产权资产管理有限公司 改进的细胞培养方法
CN102690781A (zh) * 2012-04-28 2012-09-26 苏州金盟生物技术有限公司 一种灌流式细胞培养的培养基更换方法
US20130017577A1 (en) * 2009-11-17 2013-01-17 Medarex, Inc. Methods for enhanced protein production
CN103298925A (zh) * 2010-12-21 2013-09-11 葛莱高托普有限公司 用于培养人髓样白血病细胞以及由其衍生的细胞的方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2997771B2 (ja) * 1998-06-29 2000-01-11 金沢大学長 細胞の懸濁培養方法
US20040185535A1 (en) 2003-03-21 2004-09-23 Giles Wilson Industrial-scale serum-free production of recombinant FVII in mammalian cells
US20040185534A1 (en) * 2000-10-02 2004-09-23 Knudsen Ida Molgaard Industrial-scale serum-free production of recombinant proteins in mammalian cells
WO2007071072A1 (en) 2005-12-22 2007-06-28 Corporation De L'ecole Polytechnique De Montreal High-rate perfusion bioreactor
EP2014760A1 (en) 2007-06-13 2009-01-14 CMC Biopharmaceuticals A/S A method for producing a biopolymer (e.g. polypeptide) in a continuous fermentation process
EP2599651A1 (de) 2011-12-01 2013-06-05 Magna E-Car Systems GmbH & Co OG Heiz-/Kühlsystem für eine Batterie eines Kraftfahrzeugs sowie Betriebsverfahren hierfür
CN104195187B (zh) 2014-08-01 2018-02-16 润科生物工程（福建）有限公司 沉降‑逆灌流‑放流偶联制备富含dha油脂的方法及其专用细胞沉降槽
EP3303557B1 (en) 2015-05-29 2021-05-05 Glycotope GmbH Small scale cultivation method for suspension cells

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0599651A2 (en) * 1992-11-25 1994-06-01 National Research Council Of Canada Bioreactor for the perfusion culture of cells
CN101490239A (zh) * 2006-07-14 2009-07-22 帝斯曼知识产权资产管理有限公司 改进的细胞培养方法
US20130017577A1 (en) * 2009-11-17 2013-01-17 Medarex, Inc. Methods for enhanced protein production
CN103298925A (zh) * 2010-12-21 2013-09-11 葛莱高托普有限公司 用于培养人髓样白血病细胞以及由其衍生的细胞的方法
CN102690781A (zh) * 2012-04-28 2012-09-26 苏州金盟生物技术有限公司 一种灌流式细胞培养的培养基更换方法

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111139216A (zh) * 2018-11-01 2020-05-12 上海药明生物技术有限公司 高通量、自动化的细胞连续培养方法及其应用
CN114599782A (zh) * 2019-07-01 2022-06-07 科学生物技术公司 用于在多并联生物反应器中培养粘附细胞的方法

Also Published As

Publication number Publication date
JP2018516077A (ja) 2018-06-21
WO2016193083A1 (en) 2016-12-08
JP6737813B2 (ja) 2020-08-12
EP3303557A1 (en) 2018-04-11
RU2730656C2 (ru) 2020-08-24
EP3303557B1 (en) 2021-05-05
US20180135091A1 (en) 2018-05-17
BR112017023624A2 (pt) 2018-07-17
RU2017140920A3 (https=) 2019-07-17
KR20180010200A (ko) 2018-01-30
RU2017140920A (ru) 2019-07-01
CA2985435A1 (en) 2016-12-08
AU2016272566A1 (en) 2017-11-23

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