CN108135179A - Non-toxic herb agent composition and its method and purposes - Google Patents

Non-toxic herb agent composition and its method and purposes Download PDF

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CN108135179A
CN108135179A CN201680061820.9A CN201680061820A CN108135179A CN 108135179 A CN108135179 A CN 108135179A CN 201680061820 A CN201680061820 A CN 201680061820A CN 108135179 A CN108135179 A CN 108135179A
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plant agent
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P·戴尔
P·D·戴尔
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Neozyme International Inc
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Neozyme International Inc
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Abstract

Subject description discloses plant agent composition, the virulence factor of article, the container comprising such composition or the kit of manufacture and control plant disease or increase plant growth and/or the methods and purposes of fruit production.

Description

Non-toxic herb agent composition and its method and purposes
Priority and Shen this application claims the U.S. Provisional Patent Application 62/208,662 submitted for 22nd in August in 2015 Please day equity, the content of the temporary patent application is integrally incorporated accordingly by reference with it.
Agricultural is most important for the world.Agricultural is vital not only for whole world food is provided, and Even if it has important Economic Importance for the economy of most of not all countries.It can influence agricultural crops Three factors of yield are plant diseases, unfavorable growth conditions and cultivate inefficiency.
Loss from infectious plant disease can have catastrophic humanistic influence, and wherein Crop damage causes Starvation is hard up and suffers from hunger.In addition, the loss from plant disease can also have apparent economic impact, and crop is given birth to Production person and dealer cause reduced income and higher price are caused for consumer.Infectious plant disease control wherein In the case of method processed lacks or is restricted, 30% to 50% annual loss is common for chief crop.Based on agricultural The conventional plant agent (plant agent) of chemicals has improved agricultural productivity.However, due to its negative consequences for example Such as the increased cost of consumer and the income for crop production person and dealer's reduction, the use of agricultural chemicals It has become and is out of favour.Additionally, there are cumulative public's problems about agricultural chemicals to the negative effect of environment.Therefore, It from plant disease in terms of crop yield is improved is vital to protect agriculturally important crop.
Crop plants in whole world Different ecosystems are also exposed to the health and vigor for negatively affecting plant Unfavorable growth conditions.These are less than ideal conditions typically due to soil or weather conditions or the extreme value including temperature It is various stress, the unfavorable relationship between moisture and oxygen, soil or noxious material and the excess of essential mineral matter in atmosphere Or lack.Such factor can reduce the productivity of crop or even in better growing conditions in larger or smaller degree Under.Therefore, the growth conditions for improving agriculturally important crop is important in terms of crop yield is improved.
Finally, increased population in the world increases the adjoint reduction together with the land for agricultural not only to optimization crop life Yield and to increase cultivate efficiency increase pressure.In addition, the increase only increased with world population and the agriculture land used Both reductions on ground continue, and the demand for improving crop yield will be increased.Therefore, the agriculturally life of the raising of important crop Yield is vital in terms of crop yield is improved.
Therefore, for the health of plant and the environmental-friendly processing of vigor will be increased there are great demand, no matter plant By plant disease, by the working condition of difference come stress or even when plant is healthy and/or is given birth under favorable conditions Long, but need increased cultivation efficiency and productivity.It is such to handle the amount that also reduce agricultural chemicals, if not If altogether dispensing with, to protect the happiness of the mankind and environment.
It summarizes
Plant agent composition (plant agent composition) is disclosed in terms of this specification.Disclosed plant Agent composition includes the microorganism supernatant of processed fermentation and one or more of nonionic surface active agent.It is disclosed Plant agent composition can also include one or more of anionic surfactant.Disclosed plant agent composition is can to give birth to Object degradation and be nontoxic to the mankind, mammal, plant and environment.
Plant agent kit (plant agent kit) is disclosed in terms of this specification.Disclosed plant agent kit Comprising plant agent composition disclosed herein and how using the composition come improve plant health and vigor specification.
The method that control plant disease is disclosed in terms of this specification.Disclosed method is included a effective amount of this paper public affairs The plant agent composition opened is applied to the one or more of one or more of plants and/or wherein desired control plant disease Place (location).
The side for increasing plant growth and/or fruit production (fruit production) is disclosed in terms of this specification Method.Disclosed method include by a effective amount of plant agent composition disclosed herein be applied to one or more of plants and/or Wherein it is expected one or more places for increasing plant growth and/or fruit production.
Plant agent composition is disclosed in terms of this specification for controlling the purposes of plant disease.Disclosed purposes includes A effective amount of plant agent composition disclosed herein is applied to one or more of plants and/or wherein desired control plant disease One or more places of disease.
Plant agent composition is disclosed in terms of this specification for increasing the purposes of plant growth and/or fruit production. Disclosed purposes includes a effective amount of plant agent composition disclosed herein being applied to one or more of plants and/or wherein It is expected one or more places of increased plant growth and/or fruit production.
It is described in detail
Plant becomes have disease when continuously being interfered by certain virulence factors, and the virulence factor causes to destroy plant Normal configuration, growth, function or other active abnormal physiological processes.It is this to physiological system necessary to plant or biochemistry One or more of interference of system cause characteristic pathological condition or symptom.Plant disease is for example true by pathogenic organisms Bacterium, bacterium, mycoplasma, virus, viroid, nematode or parasitic flowering plant (parasitic flowering plant) cause. Infectious agent is transferable, can be bred in its host or on its host and travel to another easily from a susceptible host Feel host.Plant disease can broadly be classified according to the property of its major virulent factor (causal agent).It is such Major virulent factor includes virus, the microorganism of such as fungi and bacterium and the animal such as nematode.However, in treatment by such A difficulty in terms of plant disease caused by major virulent factor is that they are protected against ring typically via certain structure Border influences.These protective structures are not only necessary in terms of the health for maintaining these virulence factors, but also are protecting these It is helpful in terms of compound of the virulence factor from being designed to destroy virulence factor.
The complete virion for being referred to as virion is coated by the protectiveness for being referred to as the protein of capsid The nucleic acid composition that (protective coat) is surrounded.Capsid wraps up the genetic stocks of virus and by several oligomeric constituents subunit Composition, the subunit are made of the protein for being referred to as promoter.Certain viruses are by coating, it means that capsid is with being referred to as The lipid film coating of peplos.Coating is obtained by the capsid of the intercellular membrane in virus host；Example includes inside The outer membrane of nuclear membrane, golgi's membrane and cell.
Microorganism such as such as bacterium, mycoplasma (without the bacterium of cell wall) and certain fungus secretions generally include carefully The polymer aggregation (polymeric conglomeration) of the biopolymer of extracellular nucleic acid, protein and polysaccharide, institute State the matrix that biopolymer forms extracellular polymer substance (EPS).EPS matrix be embedded in cell, cell is caused to be adhering to each other with And it is adhered to (biological) of any work or lifeless (abiotic) surface is referred to as biomembrane or rete malpighii to be formed The stockless group of microorganism.Biomembrane bacterium colony can also be on solid substrate that is in aqueous solution or being exposed to aqueous solution be submerged It is formed or is formed on liquid surface as floatation pad (mat).
There are five stages of biomembrane development, initial attachment, irreversible attachment, maturation I, maturation II and dispersion.Biology Film, which is formed initially, to be attached to surface with the plankton freely floated and starts.These first external bacterium colonies (colonists) are most Surface is just adhered to by weak reversible adhesion via Van der Waals force.If do not detached immediately from surface, then these first External bacterium colony becomes by secreting EPS matrix and forming cell adherence structure such as pili (irreversible attachment) and permanent earth anchor It is fixed.It is had begun once colonizing, biomembrane just passes through the cell division of embedded microorganism and new recruitment (ripe I and II) It combines and grows.Other than the extracellular biological polymer by microorganism secretion, biomembrane can also be incorporated to from surrounding ring The material in border, including being not limited to minerals, soil particle and biological components.Ripe I and II be wherein establish biomembrane and The stage of shapes and sizes can only be changed.The terminal stage of biofilm formation is referred to as disperseing, and wherein microorganism is from biomembrane Release is to enter the planktonic growth stage, to propagate and colonize new surface.
The microorganism to survive in biomembrane is distinguishing in a physiologically and with differing markedly from same species The property of the plankton freely floated.One for these differences is the reason is that since biomembrane protects microorganism from ring Border influences and them is allowed to cooperate and interact in many ways.For example, biomembrane increases microorganism to detergent and resists The resistance of raw element.In addition, lateral gene transfer greatly facilitates biomembrane and leads to more stable biofilm structure.Biomembrane Interior microorganism can also use the product of such as N- acetylhomoserines lactone (AHL) to lead to each other via quorum sensing (QS) Letter.Therefore, biomembrane in terms of microorganism is protected with that may completely cut off with the interaction that environment is harmful to by them by playing key And important role.
Larger microorganism can also be protected against environment influence by certain structure.Nematode has cuticula, polymerization Protein extracellular epimatrix.When most of conjunction born of the same parents cuticular celluloses for being referred to as hypodermis (hypodermis) are from its top film When (apical membrane) secretes various protein, the cuticula of nematode is formed, the protein is by peroxidase under It is widely crosslinked to form cuticula on the outer surface of skin.The key component of this flexibility cuticula is collagen superfamily and cutin The member of plain (cuticlin), a kind of protein of highly cross-linked insoluble type.Covering cuticula is rich in lipid Three layers of epicuticle, the epicuticle itself is by loose association, rich in glycoprotein, negatively charged surface coatings (or polysaccharide Coating) covering.This multi-functional extracellular structure generates the impervious barrier of height, the impervious barrier protection line of height Worm is from dehydration and pathogenic infection；And structural framing is generated, the structural framing keeps its subject form and integrality, prevents It can only move by the mechanical damage of environmental nuisance and via body-wall muscle (body-wall muscle) is attached to.Cause This, nematode cuticula is keeping the integrality of animal and its with playing crucial and important role in terms of the interaction of environment.
Therefore, protectiveness present in the major virulent factor of plant disease such as such as virus, bacterium, fungi and nematode Structure is important not only for the survival of these factors, but also protects them from environment influence.Therefore, it destroys or with it The processing of the protective structures of the major virulent factor of his mode destruction of plants disease will have very big benefit.
Plant or green plants are the plant kingdoms to form differentiation branch green plants circle (clade Viridiplantae) The multi-celled eukaryotes of (kingdom Plantae).Green plants includes flowering plant, coniferae and other naked sub- plants Object, pteridophyte, lycopods, Anthocerotae, Hepaticae, bryophyte and green alga, but exclude red algae and brown alga, fungi, Archimycetes, Bacterium and animal.Plant is characterized in that, obtains the major part of its energy from sunlight via photosynthesis using chloroplaset. Chloroplaset includes chlorophyll a and chlorophyll b, they provide the green of chloroplaset.Plant is further characterized in that there is thick fiber The cell wall of element, central vacuole used to store, for storing the plasmid body of pigment, zoogamy, modularization and uncertain life Long (modular and indeterminate growth) and the alternation of generations, although asexual reproduction is also common.
Typical plant is divided into two kinds of first-level class, root system system and dry systems in structure.Root system system is usually understood And the stem structure including taproot and lateral root and improvement such as stem tuber and root-like stock.This system serves following：By plant anchor Determine in the soil, from ground absorption water and nutrients, water and nutrients to be transported in entire plant, stores food, and generate certain A little hormones.Dry systems are usually more than ground and including stem, leaf and organ of multiplication.This system serves following：By plant liter More than height to soil, photosynthesis is carried out, is bred, water and nutrients are transported in entire plant, store food and generated Hormone.
The plant that the vascular tissue of resource is distributed included in entire plant is referred to as vascular plant.It is also known as dimension Guan Zhi The vascular plant (vascular plant) of object (tracheophyte) is defined as guiding water in entire plant With the dimension pipe of special non-woodization of the vascular tissue (xylem) and the photosynthetic product of guiding of the lignifying of minerals Organize those terrestrial plants of (bast).Vascular tissue includes lycopods, equisetales, pteridophyte, gymnosperm (including pine Cypress class) and angiosperm (flowering plant).The scientific of the group includes vascular plant and vascular plant (Tracheobionta)。
Xylem is the vascular tissue including dead cell in maturation.Xylem provides xylem sap is upward from root Unidirectional transport is unidirectionally transported to plant and in entire plant.Xylem sap includes water, soluble mineral nutrients and inorganic Ion, although xylem can also include many organic chemicals.The movement that xylem sap passes through xylem be it is passive, according to Rely in capillarity to provide the power for the balanced arrangement for establishing counteracting gravity.This capillarity mainly passes through two kinds of mechanism To realize：Transpiration pull (transpirational pull) and root pressure.Transpiration pull is due to coming from Ye Zhongxi by evaporation The surface tension that the water on the surface of born of the same parents generates, causes the negative pressure in xylem, negative pressure generation be enough by xylem sap from The power that root and soil pull up.Root pressure is since the infiltration generated by root cells relative to the more negative flow of water of soil is made With this is because causing the higher solute concentration of positive pressure, which forces xylem sap upward from xylem towards leaf.
Bast includes vascular tissue living, and the vascular tissue includes 1) guiding cell (conducting cell), It is referred to as screen element (sieve element), forms pipe；2) parenchyma cell, partner cells or albuminous cell including specialization Both with unspecialized cell；And 3) sertoli cell (supportive cell), such as fiber that machinery supports and hard is provided Change cell.Screen element does not have karyon and with considerably less organelle, therefore they are thin dependent on partner cells or albumin Born of the same parents are for its most of metabolic demand.Bast is when needed to the every other part of plant, and particularly plant is non-photosynthetic Effect partial such as root provides the photosynthate (or juice) generated by the photosynthesis region (mainly leaf) of plant Multi-direction transport or transport in memory structure such as pipe or stem.Photosynthate is the solution based on water, the solution Rich in the carbohydrate generated in photosynthesis period and other soluble organic nutrient substances.The shifting that photosynthate passes through bast It is dynamic to be driven by positive hydrostatic pressing.This process is referred to as transporting, and the process by being referred to as phloem loading and unloading To complete.Cell in sugared source by by solute molecule active transport to cell come " loading " sieve-tube elements (sieve- tube element).This causes water to be moved in screen element by osmosis, generates and pushes down on juice to the pressure of pipe Power.In sugared library (sugar sink), solute is initiatively transported out by cell from screen element, is produced a contrary effect.
Root system system is the organ for typically lying in the plant below the surface of soil.In structure, root includes epidermis, skin Matter, endothelium, pericycle and skeleton.Epidermis is the outer layer of cell.Cortex is to be incorporated in outside by epidermis and pass through Endothelial bound is in the primary structure tissue of internal root.Endothelium separates cortex and pericycle, the pericycle be side (or point Branch) root origin in tissue.It is the vascular tissue for including xylem and bast at the center of root.Root system system includes main root, side Root and Gen Mao and three vitellariums can be divided into.Maturation zone is the root system for the maturing part for including main root, lateral root and Gen Mao The part of system absorbs water and nutrients from soil and passes through water and nutrients in xylem transport to branch system.Prolong It is the cell wherein newly divided just in widened region to stretch area (zone of elongation).Meristem zone includes the tip of a root point Raw tissue and root cap and be wherein cell division and area that new cell growth occurs.
Root hair is the unicellular extension of absorbability of the epidermal cell of root.These tiny hair-like structures play water and minerals The effect of the main portions of absorption.In the presence of with the relevant beneficial microorganism of root hair, the microorganism and plant form beneficial The relationship of symbiosis.Mycorhiza is the soil fungi for seeming to extend root and the contact of soil overview (soil profile), enhancing water with Nutritious compound absorption.Rhizobium are typically via forming tubercle on the root of plant so that atmospheric nitrogen can be used for the soil of plant Bacterium.
Survival of the suitable transport of xylem sap and photosynthate (photosynthate) the two for plant It is important.Therefore, promote this transportational process by the health of useful plants.For example, improved absorption causes to plant at root hair The amount of increased water, minerals and other nutrients needed for object growth.Similarly, preferably pass through the xylem of vascular tissue Juice and photosynthate, which flow, ensures to maintain and continue the effective and efficient of compound and energy needed for plant growth Synthesis.
On the other hand, destroy or prevent any obstruction of the movement of xylem sap and photosynthate to influence plant Health.For example, the interference of transpiration pull is tempestuously reduced in xylem caused by high temperature, high humility, dark or arid Negative water pressure leads to the xylem sap flowing of difference.Similarly, pass through the difference of root hair caused by by unfavorable environmental condition The interference of the root pressure of water and nutritious compound absorption can significantly reduce the positive hydraulic pressure in xylem, lead to the xylem sap stream of difference It is dynamic.As another example, the photosynthate flowing destroyed in xylem leads to the nutrients distribution of difference.In these situations It is any in, such flow damage can cause it is listless wither, wither, the breeding of stunted growth and reduction and to plant disease and The increased sensibility of unfavorable environmental condition.About agricultural, such flow damage eventually leads to reduced crop yield.Cause This, the processing for respectively facilitating, keeping or enhancing xylem sap and the photosynthate flowing in xylem and bast is incited somebody to action With very big benefit.
Irrigation is by water manual application to land or soil.It is used to help the growth of agricultural crops, the holding of landscape with And in Arid Area and the planting again to destroyed soil during the rainfall insufficient period.It irrigates in crop production With other several purposes, including protecting plants from frost, inhibit weed growth and prevent soil consolidation.Compared to it Under, only rely upon direct rainfall agricultural be referred to as rain support (rain-fed) or nonirrigated farmland cultivation.
The target of irrigation is that entire field is equably supplied with water so that each plant has the water of its needs, neither Also it is not very little too much.Top formula (overhead) is irrigated or sprinkler irrigation is wherein under high pressure by piping network by water distribution One or more centers into field and the system being distributed by pushing up formula water sprinkler or rifle.Water sprinkler can also be by On the platform of different zones that can be manually or automatically moved to field.Centrally-pivoted axle (center pivot), Mobile water sprinkler (traveling sprinkler), lateral movement and wheel line, which irrigate (wheel line irrigation), is The type of top formula irrigation method.Localized irrigation is that wherein water is distributed simultaneously under low pressure by piping network with predetermined pattern The system of each plant or its neighbouring plant is applied to as small emission.It oozes, spray or miniature water sprinkler and blistering Device irrigation is the type of localized irrigation's method.Localized irrigation's method can be most water-saving irrigation method, because they are only delivered Required water simultaneously minimizes evaporation and runoff.
Most of business and house irrigation system are " (in ground) in ground " systems, it is meant that everything all by It is embedded in underground.In pipeline, water sprinkler, injector (emitter) and in the case that irrigation valve is hidden, this causes no garden More cleaning, the more beautiful landscape that hose (garden hose) or other articles must move back and forth manually.However, this is really Some shortcomings are generated in the system aspects that maintenance is buried completely.
Irrigation can lead to many problems.For example, since the growth of algae and other microorganisms generates biomembrane, top formula fills Irrigating the piping network of system and localized irrigation system may become to block, and lead to abnormal water distribution.Water distribution undesirable in this way It can cause to negatively affect the health of plant and the unfavorable growth conditions of vigor.For example, inconsistent water distribution cause by The irrigation of part in field caused by unequal unevenness in distribution is insufficient or heavy irrigation；Increase Soil salinity, As a result, toxic salt is accumulated on the soil surface caused by irrigating deficiency, caused by irrigating insufficient or heavy irrigation Crop failure；And increase the prevalence of plant disease.Therefore, promote, maintain or enhance localized irrigation system and top formula irrigates system The processing of water flowing will be with very big benefit in system.
It is not intended to be limited by its theory, plant agent composition disclosed by the invention dissolves, disperses or otherwise break The virulence factor of bad plant disease is one or more of groups of protective structures as present on virus, bacterium, fungi and nematode Point, lead to the death of virulence factor by destroying one or more of basic physiology courses.This mechanism of action and public affairs herein The cuticula of the capsid of virus, the biomembrane of microorganism and nematode is broken or otherwise ruptured to the plant agent composition opened The ability of film upper epidermal layer based on lipid is associated.Using the method for disclosed plant agent composition by outer exposed come real It is existing, by being applied directly to virulence factor, will become to be exposed to disclosed plant agent group indirectly by processing wherein virulence factor Close object place or with provide one or more of components of protective structures present on virulence factor be sufficiently destroyed and It is any that virulence factor is then exposed to disclosed plant agent composition by way of destroying the subsequent death of basic physiological process Other methods.
In addition, it is undesirable to it is limited by its theory, plant agent composition disclosed by the invention improves the absorption by root hair, It improves and the flowing of the photosynthate in bast is flowed and improved by the xylem sap of xylem, cause to maintain And/or the improved transport of the water and nutrients of the health and vigor of enhancing plant.This mechanism of action and plant disclosed herein Agent composition increases the absorption of water, minerals and other nutrients from soil, increase in xylem capillarity and/ Or hydrostatic pressing and/or increase the ability of synthesis of compound and energy and be associated, cause lasting and continuation plant growth and/ Or the health and vigor of the plant of enhancing.It is realized, passed through by outer exposed using the method for disclosed plant agent composition Be applied directly to plant, indirectly by processing wherein plant by become to be exposed to disclosed plant agent composition place or with The absorption for improving water, minerals and other nutrients is proposed, improves transport and/or improvement dimension of these raw material in entire plant Plant is exposed to disclosed plant agent composition by the mode of synthesis held and continue the compound and energy needed for plant growth Any other method.
Similarly, it is undesirable to be limited by its theory, plant agent composition disclosed by the invention is dissolved, disperseed or with other Mode remove destroy xylem in xylem sap flowing and/or bast in photosynthate flow one or more Component causes to maintain and/or enhance the improved transport of the water and nutrients of the health of plant and vigor.This mechanism of action It is dissolved with plant agent composition disclosed herein or otherwise removing stops the channel of (block) xylem and bast The ability of one or more of components is associated.It is realized using the method for disclosed plant agent composition by outer exposed, By being applied directly to plant, indirectly by the place that processing wherein plant will become to be exposed to disclosed plant agent composition, Or with provide blocking xylem sap and/or photosynthate flowing one or more of components be sufficiently destroyed and with Afterwards in entire plant the improved mode of water and nutrients by plant be exposed to disclosed plant agent composition any other Method.In embodiments, one or more of component packets of the flowing of blocking xylem sap and/or photosynthate flowing Include biomembrane.
In addition, it is undesirable to be limited by its theory, plant agent composition disclosed by the invention is dissolved, disperseed or with its other party Formula removes one or more of components of water flowing in the pipeline network for destroying irrigation system, causes to maintain and/or enhancing is planted The health of object and the improved water distribution of vigor.This mechanism of action dissolves with plant agent composition disclosed herein or with other The ability that mode removes one or more of components of blocks line network is associated.Using the side of disclosed plant agent composition Method is realized by outer exposed, by being applied directly to the pipeline network of irrigation system or to provide blocks line network One or more of components it is abundant remove and then in entire pipeline network the mode of improved water transport by pipe-line-network Network is exposed to any other method of disclosed plant agent composition.In embodiments, one kind of blocks line network or more Various ingredients include biomembrane.
Do not consider theory of operation, disclosed plant agent composition and method and purposes provide independent of to the mankind or The alternative means of the chemicals of environmental toxic.But plant agent composition disclosed herein and method and purposes pass through exploration Intrinsic technique works to improve the synthesis that raw material absorb and transport and improve growth and maintain compound and energy.It is similar Ground, plant agent composition disclosed herein and method and purposes by exploring natural fragility of the virulence factor to its environment, Stop water in the one or more of components or blocking irrigation system that xylem sap and/or photosynthate flow in plant One or more of components of flowing work.In addition, the component of disclosed plant agent composition is had been demonstrated to people and domestic animal It is generally nontoxic and to wild animal and environment with minimum adverse effect.
The aspect of this specification partly discloses plant agent composition.Plant agent composition disclosed herein is included through place The microorganism supernatant of the fermentation of reason and one or more of nonionic surface active agent.On the microorganism of processed fermentation Clear liquid does not have the microorganism such as yeast or bacterium of any work, and in addition without any organized enzyme, activable proenzyme (pro- ) or any enzymatic activity enzyme.In addition, plant agent composition itself does not have the microorganism such as yeast or bacterium of any work, And in addition without any organized enzyme, activable proenzyme or any enzymatic activity.
In in terms of this embodiment, plant agent composition disclosed herein includes the warp of for example, about 75% to about 99% The microorganism supernatant of the fermentation of processing and one or more of nonionic surface active agent of about 1%-25%.Implement herein In the other side of scheme, plant agent composition disclosed herein includes for example, about 80% to about 97% processed fermentation Microorganism supernatant and about 3%-20% one or more of nonionic surface active agent.In the another of this embodiment In aspect, plant agent composition disclosed herein includes the microorganism supernatant of for example, about 85% to about 95% processed fermentation One or more of nonionic surface active agent of liquid and about 5%-15%.In the other other side of this embodiment In, plant agent composition disclosed herein include for example, about 87% to about 93% processed fermentation microorganism supernatant and One or more of nonionic surface active agent of about 7%-13%.It is public herein in the other side of this embodiment The plant agent composition opened includes the microorganism supernatant and about 8%- of for example, about 88% to about 92% processed fermentation 12% one or more of nonionic surface active agent.In the other side of this embodiment, plant disclosed herein The one of microorganism supernatant of the agent composition comprising for example, about 89% to about 91% processed fermentation and about 9%-11% Kind or more kind nonionic surface active agent.
The aspect of this specification partly discloses the microorganism supernatant of fermentation.On the microorganism of fermentation disclosed herein Clear liquid can pass through culture yeasts bacterial strain, bacterium bacterial strain or yeast in the fermentation medium comprising sugared source, malt and magnesium salts It is prepared by the combination of both bacterial strain and bacterium bacterial strain.In in terms of this embodiment, only single yeast strain is in fermented and cultured It is used in base.In the other side of this embodiment, two or more different yeast strains are in the fermentation medium It uses.In the another aspect of this embodiment, only single bacterium bacterial strain uses in the fermentation medium.In this embodiment In addition in other side, two or more different bacterium bacterial strains use in the fermentation medium.In this embodiment In other side, one or more of different yeast strains are together with one or more of different bacteriums in fermentation medium Middle use.In the another aspect of this embodiment, two kinds, three kinds, four kinds, five kinds or more the different yeast strains of kind together with Two kinds, three kinds, four kinds, five kinds or more are planted different bacteriums and used in the fermentation medium.
Sugared source includes but not limited to the sucrose from molasses, raw sugar, soybean or its mixture.In addition to reduced sugar for example Except glucose and maltose and ash content, organic non-saccharide and some water, molasses are usually also comprising 50% sucrose up to about. The presence of the sugar of the type found in molasses is important in terms of their enzymes of generation and the activity of saccharomycete is promoted.Although Untreated sugarcane blackstrap molasses molasses (cane blackstrap molasses) are preferred, but can also use other sugar Sweet such as beet molasses, bucket molasses (barrel molasses) and the like are as the natural next of the material needed for enzymatic fermentation Source.In the amount for preparing molasses useful in fermentation medium disclosed herein between by weight 40% and about 80%, and Preferably between by weight about 55% and about 75%.It will be appreciated that the specific amount of the molasses used can change with Generate desired optimum combination object.
Raw sugar is the sugar product for not being refined and including remaining molasses and other natural impuritys.Although no It is clearly understood, it has however been found that with using the residue chemistry used included in decoloration and final purifying and in refining The use of the refined sugar of product is compared, and the chemicals of the remnants may have some harmful works to saccharomycete and maltase With the property that the presence generation of raw sugar significantly improves in fermentation reaction.Have been found that existing fermented material in the mixture A part include raw sugar in the case of, the optimal biological property and enzymatic property of disclosed fermentation medium are modified. Can be by weight about 10% and about 40% in the amount for preparing raw sugar useful in fermentation medium disclosed herein, and Preferably between by weight about 10% and about 30%.It will be appreciated that the specific amount of the raw sugar used can change To generate desired optimum combination object.
The key enzyme for advantageously contributing to fermentation reaction is provided by malt and yeast and/or bacterium.What is used is specific Malt is preferably diastatic malt, and the diastatic malt includes enzyme, and the enzyme includes carbohydrase, maltose and amylase.It removes Contribute to except the total effect and activity of enzymatic composition in final products mixture, malt is also believed to improve saccharomycete And/or the activity of bacterium.It can be by weight about 3% in the amount for preparing malt useful in fermentation medium disclosed herein Between about 15%, and preferably between by weight about 7% and about 12%.It will be appreciated that the tool of the malt used The amount of body can change to generate desired optimum combination object.
Fermentation be cause carbohydrate and other complicated organic material decompositions into such as sugar, acid, gas and alcohol more The metabolic process of simple substance.Fermentation can carry out in yeast, bacterium and mould.Fermentation includes alcohol fermentation and lactic acid hair Ferment.Lactic fermentation includes homofermentative lactic and heterolactic fermentation.
Yeast, which refers to generate, to be caused for example by carbohydrate-modifying into the fermentation reaction of carbon dioxide and alcohol institute Any fermented fungal of the enzyme needed.During fermentation reaction, many enzymes are generated by active dry yeasr, and including hydrolase and Both oxidizing ferment, for example, invertase, catalyzing enzyme, lactase, maltose, carboxylase and other.Yeast is included in following food Useful yeast strain in processing and fermentation：Such as the such as fermentation based on beans, the fermentation based on flour, the hair based on cereal Ferment, the fermentation based on vegetables, the fermentation based on fruit, the fermentation based on honey, the fermentation based on dairy products, the hair based on fish Ferment, the fermentation based on meat and the fermentation based on tea.The useful special Blastocystis in fermentation reaction disclosed herein Non-exhaustive inventory includes but not limited to brettanomyce Pseudomonas, Candida, intends Weir Blastocystis (Cyberlindnera), the black powder Blastocystis (Cystofilobasidium) of sporangiocyst line, Dbaly yeast Pseudomonas (Debaryomyces), moral carat saccharomycete (Dekkera), Fusarium, geotrichum sp category, her Sa Blastocystis (Issatchenkia), Kazakhstan's Blastocystis (Kazachstania), Ke Leke Blastocystis (Kloeckera), gram Shandong dimension Blastocystis, lecanium Pseudomonas (Lecanicillium), Mucor, Neurospora, Pediococcus (Pediococcus), Penicillium, Pichia pastoris Pseudomonas, Rhizopus (Rhizopus), rhodosporidium toruloides Pseudomonas (Rhodosporidium), red ferment Female Pseudomonas (Rhodotorula), Schizosccharomyces, hair spore Blastocystis (Thrichosporon), has spore at Blastocystis Torula (Torulaspora), Torulopsis, Verticillium dahliae category (Verticillium), Ye Luoweiya Blastocystis (Yarrowia), Zygosaccharomyces Pseudomonas (Zygosaccharomyces) or Zygotorulaspora.Fermentation disclosed herein is anti- Useful yeast species belong to but are not limited in answering, and useful special yeast species is non-detailed in fermentation reaction disclosed herein Most inventory includes but not limited to abnormal British mold (B.anomalus), Brussels British mold (B.bruxellensis), gram Lawson British mold (B.claussenii), Ban Tute British molds (B.custersianus), Na Shi British molds (B.naardenensis), this southern brettanomyce (B.nanus), mound shape are false Silk yeast (C.colliculosa), small Candida (C.exiguous), Candida humicola (C.humicola), koumiss Candidiasis (C.kefyr), candida krusei (C.krusei), Miller Candida (C.milleri), Candida Bacterium (C.mycoderma), candida pelliculosa bacterium (C.pelliculosa), corrugated candidiasis (C.rugose), star Candidiasis (C.stellate), Candida tropicalis (C.tropicalis), candida albicans bacterium of laying eggs (C.utilis), Candida valida bacterium (C.valida), sour wine candidiasis (C.vini), candida zeylanoides bacterium (C.zeylanoides), it is inferior to intend Weir killer yeast bacterium (Cb.mrakii), Cryptococcus (Cs, infirmominiatum), the Chinese Dbaly yeast bacterium (D.hansenii), Ke Leke saccharomyces pastorianus bacterium (D.kloeckeri), abnormal moral carat saccharomycete (Dk.anomala), Brussels moral gram yeast (Dk.bruxellensis), F.domesticum, Geotrichum (G.candidum), Issatchenkia orientalis bacterium (I.orientalis), marine yeast (K.exigua), monospore saccharomyces cerevisiae (K.unispora), Africa Ke Leke yeast (KI.africana), K. apiculata bacterium (KI.apis), Java gram Strangle gram yeast (KI.javanica), lactic acid yeast kluyveromyces (Ku.lactis), Kluyveromyces marxianus bacterium (Ku.marxianus), Kluyveromyces marxianus bacterium (Ku.marxianus), Verticillium lecanii (L.lecanii), frozen soil hair Mould (M.hiemalis), thicket Mucor (M.plumbeus), Mucor racemosus (M.racemosus), Mucor racemosus, type arteries and veins Born of the same parents bacterium (N.intermedia), pediococcus cerevisiae (P.cerevisiae), white Penicillium notatum (Pn.album), sramana cypress cheese are green It is mould (Pn.camemberti), cheese Penicillium notatum (Pn.caseifulvum), penicillium chrysogenum (Pn.chrysogenum), general Logical Penicillium notatum (Pn.commune) receives ground mould (Pn.nalgiovense), penicillium roqueforti (Pn.roqueforti), chlorine nitrogen Tall and erect Penicillium notatum (Pn.solitum), fermentation Pichia yeast (Pi.fermentans), Rhizopus microsporus (R.microspores), Rs.infirmominiatum, sticky red rhodotorula (Rt.glutinis), small Rhodotorula sp (Rt.minuta), deep red saccarom ycete (Rt.rubra), saccharomyces bayanus bacterium (S.bayanus), Bu Ladi saccharomycete (S.boulardii), saccharomyces carlsbergensis bacterium (S.carlsbergensis), S. cervisiae (S.cerevisiae), true saccharomyces bayanus bacterium (S.eubayanus), unusual wine brewing Saccharomycete (S.paradoxus), saccharomyces pastorianus bacterium (S.pastorianus), methamidophos (S.rouzii), saccharomyces uvarum There are spore circle in bacterium (S.uvarum), schizosaccharomyces pombe bacterium (Sc.pombe), Fructus Atriplicis Sibiricae trichosporon bacteria (Th.beigelii), Dell Saccharomycete (T.delbrueckii), T.franciscae, ball have spore torula bacterium (T.pretoriensis), small ellipse to have spore Torula bacterium (T.microellipsoides), spherical shape have spore torula bacterium (T.globosa), India's Tilletia foetida (T.indica), Mary has spore torula bacterium (T.maleeae), T.quercuum, variable plan ball saccharomycete (To.versatilis), Verticillium lecanii (V.lecanii), Yarrowialipolytica bacterium (Y.lipolytica), Bayer connect Close saccharomycete (Z.bailii), two spore Zygosaccharomyces bacterium (Z.bisporus), aroma-producing yeast bacterium (Z.cidri), fermentation engagement ferment Female bacterium (Z.fermentati), Florence Zygosaccharomyces bacterium (Z.florentinus), engagement sugar yeast bacterium (Z.kombuchaensis), adhesive bond saccharomycete (Z.lentus), honey Zygosaccharomyces bacterium (Z.mellis), small ellipse connect Close saccharomycete (Z.microellipsoides), Mu Lake Zygosaccharomyces bacterium (Z.mrakii), vacation Lu Shi combining yeast bacterium (Z.pseudorouxii) and Lu Shi Zygosaccharomyces bacterium (Z.rouxii) and Zt.florentina.Preferred yeast is usual The saccharomyces cerevisiae (Saccharomyces cerevisiae) that can be obtained as Saccharomyces cerevisiae.
Bacterium refers to that the hair for leading to for example to generate alcohol such as ethyl alcohol or acid such as acetic acid, lactic acid and/or succinic acid can be generated Any zymogenous bacteria of enzyme needed for ferment reaction.What useful specific bacterium belonged in fermentation reaction disclosed herein is non-exhaustive Inventory include but not limited to acetobacter (Acetobacter), Arthrobacter, Aerococcus (Aerococcus), Bacillus, Bifidobacterium, short shape Bacillus (Brachybacterium), brevibacterium, Barnobacterium, meat Bacillus (Carnobacterium), rodlike Pseudomonas, enterococcus spp, Colibacter, glucose acetobacter (Gluconacetobacter), Gluconobacter, Hafnia (Hafnia), Halomonas (Halomonas), Cook Pseudomonas (Kocuria), Lactobacillus, lactococcus, Leuconostoc, megacoccus category (Macrococcus), Microbacterium, Micrococcus, neisseria, wine Coccus (Oenococcus), Pediococcus, Propionibacterium, Proteus, false monospore Pseudomonas, Psychrobacter (Psychrobacter), Salmonella, gemma Bacillus acidi lactici (Sporolactobacillus), grape Coccus, streptococcus, streptomyces, Tetracoccus (Tetragenococcus), Vagococcus (Vagococcus), Wei Si Bordetella (Weissella) and zymomonas.Useful specific bacterium object in fermentation reaction disclosed herein Kind non-exhaustive inventory include but not limited to acetify acetobacter (A.aceti), aphid parasite acetobacter (A.fabarum), Luo Wang acetobacters (A.lovaniensis), apple acetobacter (A.malorum), east acetobacter (A.orientalis), Acetobacter pasteurianus (A.pasteurianus), Acetobacter pasteurianus (A.pasteurianus), A.pomorum, Portugal acetobacter (A.syzygii), tropical acetobacter (A.tropicalis), A Shi arthrobacteriums (Ar.arilaitensis), Bei Reershi arthrobacteriums (Ar.Bergerei), Arthrobacter globiformis (Ar.Globiformis), tobacco Arthrobacterium (Ar.nicotianae), changeable arthrobacterium (Ar.variabilis), Bacillus cercus (B.cereus, condensation bud Spore bacillus (B.coagulans), bacillus licheniformis (B.licheniformis), bacillus pumilus (B.pumilus), ball Shape bacillus (B.sphaericus), bacillus stearothermophilus (B.stearothermophilus), bacillus subtilis (B.subtilis), bifidobacterium adolescentis (B.adolescentis), animal bifidobacteria (B.animalis), not tally bifid bar Bacterium (B.bifidum), bifidobacterium breve (B.breve), bifidobacterium infantis (B.infantis), lactic acid Bifidobacterium (B.lactis), bifidobacterium longum (B.longum), bifidobacterium pseudolongum (B.pseudolongum), bifidobacterium thermophilum (B.thermophilum), the small brevibacterium of food (Br.alimentarium), the small brevibacterium of food (Br.alimentarium), Cheese ferment small brevibacterium (Br.tyrofermentans), cheese ferment small brevibacterium (Br.tyrofermentans), Bv.aurantiacum, brevibacterium casei (Bv.casei), Brevibacterium linens (Bv.linens), meat bacillus is blazoned (C.divergens), maltose meat bacillus (C.maltaromaticum), the flesh of fish bacillus (C.piscicola) that dwells, production ammonia are short Bacillus (C.ammoniagenes), cheese corynebacteria (Co.casei), micro- yellow corynebacteria (Co.flavescens), the stick completely cut off Shape bacillus (Co.mooreparkense), variation corynebacteria (Co.variabile), enterococcus faecalis (E.faecalis), dung intestines ball Bacterium (E.faecium), fixed nitrogen glucose acetobacter (G.azotocaptans), acetobacter diazotrophicus acidfast bacilli (G.diazotrophicus), circus glucose acetobacter (G.entanii), European glucose acetobacter (G.europaeus), Chinese Xun Shi glucose acetobacter (G.hansenii), Johanna's glucose acetobacter (G.johannae), wooden glucose acetobacter (G.oboediens), wooden grape vinegar acidfast bacilli (G.xylinus), Gluconobacter oxvdans (Gl.oxydans), honeycomb are breathed out Husband Buddhist nun bacterium (H.alvei), elongation Halomonas (Hl.elongate), thermophilic cock Salmonella (K.rhizophila), thermophilic cock Salmonella (K.rhizophila), variation Kocuria kristinae ad (K.varians), variation Kocuria kristinae ad (K.varians), acidproof Bacillus acidi lactici (L.acidifarinae), sour powder Bacillus acidi lactici (L.acidifarinae), Lactobacillus acidophilus (L.acidipiscis), disappear Change Bacillus acidi lactici (L.alimentarius), short Bacillus acidi lactici (L.brevis), Bu Shi Bacillus acidi lacticis (L.bucheri), cocoa Bacillus acidi lactici (L.cacaonum), Lactobacillus casei (L.casei), cellobiose Bacillus acidi lactici (L.cellobiosus), mound Shape bacterium colony Bacillus acidi lactici (L.collinoides), compound Bacillus acidi lactici (L.composti), rod-shaped lactobacillus (L.coryniformis), curling Bacillus acidi lactici (L.crispatus), lactobacillus curvatus (L.curvatus), De Shi lactic acid Bacillus, dextrin Bacillus acidi lactici (L.dextrinicus), Di Shi Bacillus acidi lacticis (L.diolivorans), double enzyme fusiform Bacillus acidi lacticis (L.fabifermentans), sausage Bacillus acidi lactici (L.farciminis), fermentation lactobacillus (L.fermentum), grignard Bacillus acidi lactici (L.gasseri), Ghana's Bacillus acidi lactici (L.ghanensis), Kazakhstan Bacillus acidi lactici (L.hammesii), Harbin Bacillus acidi lactici (L.harbinensis), Lactobacillus helveticus (L.helveticus), Xi Shi Bacillus acidi lacticis (L.hilgardii), Homotype corruption wine Bacillus acidi lactici (L.homohiochii), Zhan Shi Bacillus acidi lacticis (L.jensenii), Yue Shi Bacillus acidi lacticis (L.johnsonii), koumiss sample Bacillus acidi lactici (L.kefiranofaciens), Kefir grains Bacillus acidi lactici (L.kefiri), bubble Dish Bacillus acidi lactici (L.kimchi), L.kisonensis, Kun Shi Bacillus acidi lactici (L.kunkeei), Malian Bacillus acidi lactici (L.mali), food cassava Bacillus acidi lactici (L.manihotivorans), bright step on Bacillus acidi lactici (L.mindensis), mucous membrane lactic acid Bacillus (L.mucosae), Na Shi Bacillus acidi lacticis (L.nagelii), south Mu Shi (L.namuresis), the special Bacillus acidi lactici in south (L.nantesis), Deng Shi Bacillus acidi lacticis (L.nodensis), L.oeni, L.otakiensis, bread Bacillus acidi lactici are exerted (L.panis), the short Bacillus acidi lactici of class (L.parabrevis), class Bu Shi Bacillus acidi lacticis (L.parabuchneri), class cheese breast Acidfast bacilli (L.paracasei), class kefir Bacillus acidi lactici (L.parakefiri), class digestion Bacillus acidi lactici (L.paralimentarius), class lactobacillus plantarum (L.paraplantarum), pentose Bacillus acidi lactici (L.pentosus), L.perolens, lactobacillus plantarum (L.plantarum), the sub- Bacillus acidi lactici of rag (L.pobuzihii), bridge Bacillus acidi lactici (L.pontis), L.rapi, reuteri (L.reuteri), rhamnose lactic acid bacteria (L.rhamnosus), red breast Acidfast bacilli (L.rossiae), picogram Bacillus acidi lactici (L.sakei), saliva Bacillus acidi lactici (L.salivarius), San Francisco lactic acid Bacillus (L.sanfranciscensis), Sa rub Bacillus acidi lactici (L.satsumensis), L.secaliphilus, L.senmaizukei, L.siliginis, nematode Bacillus acidi lactici (L.similis), lactobacillus reuteri (L.spicheri), The double mortar Bacillus acidi lactici (L.suebicus) of pig, L.sunkii, L.tucceti, cowpox Bacillus acidi lactici (L.vaccinostercus), Wen's Bacillus acidi lactici (L.versmoldesis), Witter not La Lishi Bacillus acidi lacticis (L.yamanashiensis, Lactococcus lactis (Lc.lactis), lactococcus raffinolactis (Lc.raffinolactis), leukonid (Le.carnosum), the bright string of thermophilic citric acid Pearl bacterium (Le.citreum), bright string coccus (Le.fallax), leuconostoc lactis (Le.holzapfelii), Le.inhae, pickles Leukonid (Le.kimchi), lactic acid leukonid (Le.lactis), Leuconostoc mesenteroides (Le.mesenteroides), palm fibre Palmitic acid leukonid (Le.palmae), leuconostoc pseudomesenteroides (Le.Pseudomesenteroides), the big coccus of molten junket (M.caseolyticus), blade microbacterium (Mb.foliorum), Mb gubbeenense, micrococcus luteus (Mc.luteus), Micrococcus lylae (Mc.lylae), Pediococcus acidilactici (P.acidilactici), Pediococcus pentosaceus (P.pentosaceus), sour propionibacterium acide-propionici (P.acidipropionici), Fei Shi propionic acid bars are produced (P.freudenreichii), propionibacterium jensenii (P.jensenii), Te Shi Propionibacteriums (P.thoenii), common variation Coccus (Pr.vulgaris), Pseudomonas fluorescens (Ps.fluorescens), Py.celer, meat streptomycete (S.carnosus), condiment staphylococcus (S.condiment), Staphylococcus equorum (S.equorum), Fu Shi staphylococcuses (S.fleurettii), fish fermented grape coccus (S.piscifermentans), staphylococcus saprophyticus (S.saphrophyticus), Staphylococcus sciuri (S.sciuri), simulation staphylococcus (S.simulans), amber grape ball Bacterium (S.succinus), calf staphylococcus (S.vitulinus), Warner staphylococcus (S.warneri), staphylococcus xylosus (S.xylosus), streptococcus cremoris (St.cremoris), gallic acid streptococcus (St.gallolyticus), saliva hammer Bacterium (St.salivarius), streptococcus thermophilus (St.thermophiles) detest color streptomycete (St.griseus), thermophilic salt tetrad Coccus (T.halophilus), South Korea's tetrads (T.koreensis), Benin Wei Si Salmonellas (W.beninensis), cibarium Wei Si Salmonellas (W.cibaria), W.fabaria, W.ghanesis, South Korea Wei Si Salmonellas (W.koreensis), class goldbeater's skin Wei Si Salmonella (W.paramesenteroides), Thailand Wei Si Salmonellas (W.thailandensis) and zymomonas mobilis (Z.mobilis)。
Mould refers to that the hair for leading to for example to generate alcohol such as ethyl alcohol or acid such as acetic acid, lactic acid and/or succinic acid can be generated Any fermentation mould of enzyme needed for ferment reaction.What useful specific mould belonged in fermentation reaction disclosed herein is non-exhaustive Inventory includes but not limited to aspergillus.The non-exhaustive inventory of useful specific mould species in fermentation reaction disclosed herein Including but not limited to A.acidus, aspergillus fumigatus, black-koji mould, aspergillus oryzae and soy sauce koji mould (A.sojae).
It will be appreciated that the actual amount of the various types of enzymes generated will depend on many factors, it is included in preparation fermentation The molasses and the type of sugar used in mixture.However, again, it is believed that in molasses and raw sugar are used, obtain optimal enzyme and receive Rate and activity.It in embodiments, can be by weight in the amount for preparing yeast useful in fermentation medium disclosed herein Between meter about 0.2% and about 5%, and preferably between by weight about 1% and about 3%.It will be appreciated that the ferment used Female specific amount can change to generate desired optimum combination object.
The presence of a small amount of organic catalyst such as magnesium salts is not only during fermentation reaction, but also hereafter in product composition In trap and decompose organic waste materials in improve enzyme activity.Preferred magnesium salts is magnesium sulfate.It is disclosed herein preparing The amount of useful magnesium salts can be between by weight about 0.1% and about 5%, and preferably by weight in fermentation medium Between meter about 1% and about 3%.It will be appreciated that the Specific amounts of the magnesium salts used can change to generate desired optimum combination Object.
In order to prepare the microorganism supernatant of fermentation, molasses, sucrose and magnesium salts are added into the warm water suitably measured.To the greatest extent The Specific amounts for the water that pipe uses is not especially important, but the water typically suitably measured is the hair used from fermentation reaction About the 2 of the total weight of the other compositions of ferment culture medium are again to about 20 times.The water of this amount is enough to promote easily to mix and foot To activate yeast, bacterium and/or mould and dissolve other materials.In addition, the temperature of water cannot be too hot so that heat inactivation fermentation Required maltase and yeast enzyme.Thus, for example greater than about 65 DEG C of coolant-temperature gage must be avoided by and preferred temperature is about Between 25 DEG C to about 45 DEG C.The use of cold water may lead to excessively slowly fermentation reaction rate, and therefore it is expected to increase Reaction rate in the case of, should also avoid the use of cold water.Molasses, sugar and magnesium salts are effectively being mixed and dissolving it Afterwards, malt and yeast are added, stir mixture and allows to cure, it is substantially complete until fermenting.At about 20 DEG C and about 45 Temperature between DEG C, reaction time can be between about 2 days and about 5 days.The effervesce of attentive response mixture can be passed through (effervescence) generally subside come after the completion of being readily determined.At the end of fermentation reaction, by micro- life of fermentation Object culture centrifuges to remove " sludge (sludge) " that is formed during fermentation.Collect the fermented supernatant fluid generated (typically By weight about 90% to about 98%) for subsequent processing.
The microorganism supernatant of fermentation includes biological nutrients, minerals and amino acid.Biological nutrients typically with from The amount of about 0.01% to about 1% of the total weight of the microorganism supernatant of fermentation exists.Each individual biological nutrients is typical Ground with from the amount of about 0.00001% to about 0.01% of the total weight of the microorganism supernatant of fermentation exist.Biological nutrients Example includes but not limited to biotin, folic acid, glucan such as alpha-glucans and beta glucan, niacin, inositol (insotil), general Acid, pyridoxol, riboflavin and thiamine.In in terms of this embodiment, the microorganism supernatant of fermentation disclosed herein Biotin comprising for example, about 0.00001% to about 0.0011%, the folic acid of about 0.0006% to about 0.016%, about 0.005% The inositol of niacin, about 0.01% to about 1% to about 15%, the pantothenic acid of about 0.00017% to about 0.017%, about 0.0006% The riboflavin of pyridoxol, about 0.002% to about 0.023% and the thiamines of about 0.001% to about 0.02% to about 0.016% Element.In in terms of other of this embodiment, the microorganism supernatant of fermentation disclosed herein include for example, about 0.00006% to About 0.0006% biotin, the folic acid of about 0.001% to about 0.011%, the niacin of about 0.01% to about 0.1%, about The inositol of 0.08% to about 0.18%, the pantothenic acid of about 0.002% to about 0.012%, about 0.001% to about 0.011% pyrrole tremble Alcohol, the riboflavin of about 0.007% to about 0.017%, about 0.003% to about 0.013% thiamine.In this embodiment again In other aspects, the microorganism supernatant of fermentation disclosed herein includes the biology of for example, about 0.00012% to about 0.0006% The flesh of element, the folic acid of about 0.001% to about 0.011%, the niacin of about 0.01% to about 0.1%, about 0.08% to about 0.18% Alcohol, the pantothenic acid of about 0.003% to about 0.013%, the pyridoxol of about 0.001% to about 0.011%, about 0.008% are to about The thiamine of 0.017% riboflavin, about 0.003% to about 0.013%.This embodiment it is other in terms of him in, herein The microorganism supernatant of disclosed fermentation includes the biotin of for example, about 0.00009% to about 0.0003%, about 0.004% to about 0.008% folic acid, the niacin of about 0.03% to about 0.07%, the inositol of about 0.11% to about 0.15%, about 0.006% are to about 0.01% pantothenic acid, the pyridoxol of about 0.004% to about 0.008%, the riboflavin of about 0.01% to about 0.014%, about The thiamine of 0.006% to about 0.010%.
Minerals typically with from the amount of about 0.1% to about 20% of the total weight of the microorganism supernatant of fermentation exist. Each individual minerals is typically deposited with the amount from about 0.0001% to about 5% of the total weight of the microorganism supernatant of fermentation .The example of minerals includes but not limited to calcium, chromium, copper, iron, magnesium, phosphate, potassium, sodium and zinc.In the side of this embodiment In face, the microorganism supernatant of fermentation disclosed herein include the calcium of for example, about 0.02% to about 0.3%, about 0.000002% to About 0.0016% chromium, the copper of about 0.000009% to about 0.0014%, the iron of about 0.00005% to about 0.02%, about The magnesium of 0.001% to about 1.3%, the phosphate of about 0.2% to about 14%, the potassium of about 0.4% to about 16%, about 0.2% are to about The zinc of 15% sodium and about 0.08% to about 13%.In in terms of other of this embodiment, fermentation disclosed herein it is micro- Biological supernatant includes the calcium of for example, about 0.07% to about 0.21%, the chromium of about 0.000007% to about 0.0011%, about The copper of 0.00004% to about 0.0009%, the iron of about 0.0001% to about 0.015%, the magnesium of about 0.005% to about 0.9%, about The phosphate of 0.7% to about 9%, the potassium of about 0.9% to about 11%, the sodium of about 0.7% to about 10% and about 0.3% are to about 8% zinc.This embodiment again in terms of other in, the microorganism supernatant of fermentation disclosed herein includes for example, about The calcium of 0.05% to about 1%, the chromium of about 0.0001% to about 0.0009%, the copper of about 0.00006% to about 0.0007%, about The iron of 0.0001% to about 0.013%, the magnesium of about 0.005% to about 1%, the phosphate of about 0.1% to about 7%, about 0.5% to The zinc of about 9% potassium, the sodium of about 0.5% to about 8% and about 0.5% to about 6%.In the other in terms of other of this embodiment In, the microorganism supernatant of fermentation disclosed herein includes the calcium of for example, about 0.12% to about 0.16%, about 0.0002% to about 0.0006% chromium, the copper of about 0.00009% to about 0.0004%, the iron of about 0.0006% to about 0.01%, about 0.01% to About 0.4% magnesium, the phosphate of about 1% to about 4%, the potassium of about 2% to about 6%, the sodium of about 1% to about 5% and about 0.8% To about 3% zinc.
Amino acid typically with from the amount of about 20% to about 60% of the total weight of the microorganism supernatant of fermentation exist.Often Individual amino acid is planted typically to exist from the amount of about 0.1% to about 15% of the total weight of the microorganism supernatant of fermentation. The example of minerals include but not limited to alanine, arginine, aspartic acid, cysteine, glutamic acid, glycine, lysine, Methionine, phenylalanine, proline, serine and threonine.In in terms of this embodiment, fermentation disclosed herein Microorganism supernatant include the alanine of for example, about 0.2% to about 16%, the arginine of about 0.09% to about 15%, about The aspartic acid of 0.4% to about 18%, the cysteine of about 0.003% to about 5%, the glutamic acid of about 0.5% to about 20%, about The glycine of 0.09% to about 15%, the lysine of about 0.09% to about 15%, the methionine of about 0.002% to about 5%, about The phenylalanine of 0.09% to about 15%, the proline of about 0.09% to about 15%, about 0.09% to about 15% serine with And the threonine of about 0.09% to about 15%.In in terms of other of this embodiment, on the microorganism of fermentation disclosed herein Clear liquid includes the alanine of for example, about 0.7% to about 11%, the arginine of about 0.5% to about 10%, about 0.9% to about 13% Aspartic acid, the cysteine of about 0.008% to about 1.2%, the glutamic acid of about 1% to about 15%, about 0.5% to about 10% Glycine, the lysine of about 0.8% to about 12%, the methionine of about 0.2% to about 1.6%, about 0.5% to about 10% benzene Alanine, the proline of about 0.5% to about 10%, the serine of about 0.5% to about 10% and about 0.5% to about 10% Soviet Union Propylhomoserin.This embodiment again in terms of other in, the microorganism supernatant of fermentation disclosed herein include for example, about 0.5% to About 9% alanine, the arginine of about 0.5% to about 8%, the aspartic acid of about 1% to about 11%, about 0.01% to about 2% Cysteine, the glutamic acid of about 3% to about 13%, the glycine of about 0.5% to about 8%, about 1% to about 10% bad ammonia Acid, the methionine of about 0.3% to about 3%, the phenylalanine of about 0.5% to about 7%, about 0.5% to about 7% proline, The threonine of the serine of about 0.5% to about 7% and about 0.5% to about 7%.In the other in terms of other of this embodiment In, the microorganism supernatant of fermentation disclosed herein includes the essence of the alanine of for example, about 2% to about 6%, about 1% to about 5% Propylhomoserin, the aspartic acid of about 4% to about 8%, the cysteine of about 0.03% to about 0.7%, about 6% to about 10% paddy ammonia Acid, the glycine of about 1% to about 5%, the lysine of about 3% to about 7%, the methionine of about 0.7% to about 1.1%, about 1% The proline of phenylalanine, about 1% to about 5%, the serine of about 1% to about 5% and about 1% to about 5% to about 5% Threonine.
The aspect of this specification partly discloses the microorganism supernatant of processed fermentation.Processed fermentation it is micro- Biological supernatant is to be denaturalized, kill or other modes destroy the yeast of any remaining work, the work contributed by yeast and malt Property enzyme and microorganism supernatant by fermentation disclosed herein present in another any other microorganism come source contribution The supernatant that the mode of body or enzyme is handled.The non-limiting examples of useful processing routine include the use of the boiling process of high temperature； High-pressure treatment process using high temperature and high pressure or the irradiation technique by the way that supernatant to be exposed to ionising radiation；Or it is denaturalized, kills Dead or other modes destroy the yeast of any remaining work, the organized enzyme contributed by yeast and malt and by hairs disclosed herein Another kind present in the microorganism supernatant of ferment comes any other microorganism of source contribution or any other sterilization process of enzyme. In addition, treatment process above can be used alone, it is in combination with one another or with pasteurization technique, chemical sterilization technique and Sterilising filtration process combination uses, to be denaturalized, kill or otherwise destroy present in fermented supernatant fluid disclosed herein Protein such as enzyme and microorganism such as yeast, bacterium and/or mould.All methods discussed above are ordinary skills Technique known to personnel, the field because they prepare and/or sterilize commonly used in food.
Then the microorganism supernatant of processed fermentation can store in liquid form, for subsequent use.It is optional Ground is selected, the microorganism supernatant of processed fermentation can be spray-dried to generate xeraphium by methods known in the art End.Dry powder form can also be stored for subsequent use.
The microorganism supernatant of the processed fermentation disclosed herein of any amount can be used for disclosed plant agent combination In object, condition is that the amount is useful to putting into practice method disclosed herein.For determining that proper amount of factor is included for example through place The microorganism supernatant of the fermentation of reason whether in liquid or powder type, the microorganism supernatant of processed fermentation specific quotient Industry source, for producing the ad hoc approach of the microorganism supernatant of processed fermentation, plant agent composition, whether conduct concentrates Object or as off-the-shelf produce and when by concentrate preparation plant agent composition when desired dilution factor.In general, phase For dry powder form, it would be desirable to the microorganism supernatant of the processed fermentation of larger amount of liquid form.
In in terms of this embodiment, the amount of the microorganism supernatant of the processed fermentation used is for example by weight Meter about 0.5%, by weight about 1.0%, by weight about 1.5%, by weight about 2.0%, by weight about 2.5%, press Weight meter about 3.0%, by weight about 3.5%, by weight about 4.0%, by weight about 4.5%, by weight about 5.0%th, by weight about 6.0%, by weight about 7.0%, by weight about 7.5%, by weight about 8.0%, by weight Meter about 9.0% or by weight about 10.0%.In in terms of other of this embodiment, micro- life of the processed fermentation used The amount of object supernatant is such as by weight at least 0.5%, by weight at least 1.0%, by weight at least 1.5%, by weight Gauge at least 2.0%, by weight at least 2.5%, by weight at least 3.0%, by weight at least 3.5%, by weight At least 4.0%, by weight at least 4.5%, by weight at least 5.0%, by weight at least 6.0%, by weight at least 7.0%th, by weight at least 7.5%, by weight at least 8.0%, by weight at least 9.0% or by weight at least 10.0%.This embodiment again in terms of other in, the amount of the microorganism supernatant of the processed fermentation used is for example By weight at most 0.5%, by weight at most 1.0%, by weight at most 1.5%, by weight at most 2.0%, by weight Gauge at most 2.5%, by weight at most 3.0%, by weight at most 3.5%, by weight at most 4.0%, by weight At most 4.5%, by weight at most 5.0%, by weight at most 6.0%, by weight at most 7.0%, by weight at most 7.5%th, by weight at most 8.0%, by weight at most 9.0% or by weight at most 10.0%.In this embodiment In addition in other aspects, the amount of the microorganism supernatant of the processed fermentation used for example by weight about 0.1% to by Between weight meter about 2.5%, between by weight about 0.1% to by weight about 3.0%, by weight about 0.1% to Between by weight about 3.5%, between by weight about 0.1% to by weight about 4.0%, by weight about 0.1% Between to by weight about 5.0%, between by weight about 0.5% to by weight about 2.5%, by weight about Between 0.5% to by weight about 3.0%, between by weight about 0.5% to by weight about 3.5%, by weight Between about 0.5% to by weight about 4.0%, between by weight about 0.5% to by weight about 5.0%, by weight Meter about 1% to by weight about 2.5% between, between by weight about 1% to by weight about 3.0%, by weight Between about 1% to by weight about 3.5%, between by weight about 1% to by weight about 4.0%, by weight about Between 1% to by weight about 5.0%, between by weight about 1% to by weight about 6.0%, by weight about 1% Between to by weight about 7.0%, between by weight about 1% to by weight about 8.0%, by weight about 1% to Between by weight about 9.0% or between by weight about 1% to by weight about 10.0%.
In in terms of other of this embodiment, the amount of the microorganism supernatant of the processed fermentation used be for example by Weight meter about 15.0%, by weight about 20.0%, by weight about 25.0%, by weight about 30.0%, by weight about 35.0%th, by weight about 40.0% it, by weight about 45.0%, by weight about 50.0%, by weight about 55.0%, presses Weight meter about 60.0%, by weight about 65.0%, by weight about 70.0%, by weight about 75.0%, by weight about 80.0%th, by weight about 85.0% or by weight about 90.0%.This embodiment again in terms of other in, the warp that uses The amount of the microorganism supernatant of the fermentation of processing is such as by weight at least 15.0%, by weight at least 20.0%, by weight Gauge at least 25.0%, by weight at least 30.0%, by weight at least 35.0%, by weight at least 40.0%, by weight Gauge at least 45.0%, by weight at least 50.0%, by weight at least 55.0%, by weight at least 60.0%, by weight Gauge at least 65.0%, by weight at least 70.0%, by weight at least 75.0%, by weight at least 80.0%, by weight Gauge at least 85.0% or by weight at least 90.0%.This embodiment it is other in terms of other in, use through processing Fermentation microorganism supernatant amount be such as by weight at most 15.0%, by weight at most 20.0%, by weight At most 25.0%, by weight at most 30.0%, by weight at most 35.0%, by weight at most 40.0%, by weight At most 45.0%, by weight at most 50.0%, by weight at most 55.0%, by weight at most 60.0%, by weight At most 65.0%, by weight at most 70.0%, by weight at most 75.0%, by weight at most 80.0%, by weight At most 85.0% or by weight at most 90.0%.
In in terms of other of this embodiment, the amount of the microorganism supernatant of the processed fermentation used for example by Between weight meter about 5% to by weight about 7.5%, between by weight about 5% to by weight about 10%, by weight Meter about 5% to by weight about 15% between, between by weight about 5% to by weight about 20%, by weight about Between 5% to by weight about 25%, between by weight about 5% to by weight about 30%, by weight about 5% to Between by weight about 35%, between by weight about 5% to by weight about 40%, by weight about 5% to by weight Between gauge about 45%, between by weight about 5% to by weight about 50%, by weight about 5% to by weight Between about 55%, between by weight about 5% to by weight about 60%, by weight about 5% to by weight about Between 65%, between by weight about 5% to by weight about 70%, by weight about 5% to by weight about 75% Between, between by weight about 5% to by weight about 80%, between by weight about 5% to by weight about 85%, Between by weight about 5% to by weight about 90%, between by weight about 5% to by weight about 95%, by Between weight meter about 10% to by weight about 15%, between by weight about 10% to by weight about 20%, by weight Between gauge about 10% to by weight about 25%, between by weight about 10% to by weight about 30%, by weight Meter about 10% to by weight about 35% between, between by weight about 10% to by weight about 40%, by weight Between about 10% to by weight about 45%, between by weight about 10% to by weight about 50%, by weight about Between 10% to by weight about 55%, between by weight about 10% to by weight about 60%, by weight about Between 10% to by weight about 65%, between by weight about 10% to by weight about 70%, by weight about Between 10% to by weight about 75%, between by weight about 10% to by weight about 80%, by weight about Between 10% to by weight about 85%, between by weight about 10% to by weight about 90%, by weight about Between 10% to by weight about 95%, between by weight about 15% to by weight about 20%, by weight about Between 15% to by weight about 25%, between by weight about 15% to by weight about 30%, by weight about Between 15% to by weight about 35%, between by weight about 15% to by weight about 40%, by weight about Between 15% to by weight about 45%, between by weight about 15% to by weight about 50%, by weight about Between 15% to by weight about 55%, between by weight about 15% to by weight about 60%, by weight about Between 15% to by weight about 65%, between by weight about 15% to by weight about 70%, by weight about Between 15% to by weight about 75%, between by weight about 15% to by weight about 80%, by weight about Between 15% to by weight about 85%, between by weight about 15% to by weight about 90%, by weight about Between 15% to by weight about 95%, between by weight about 25% to by weight about 25%, by weight about Between 25% to by weight about 30%, between by weight about 25% to by weight about 35%, by weight about Between 25% to by weight about 40%, between by weight about 25% to by weight about 45%, by weight about Between 25% to by weight about 50%, between by weight about 25% to by weight about 55%, by weight about Between 25% to by weight about 60%, between by weight about 25% to by weight about 65%, by weight about Between 25% to by weight about 70%, between by weight about 25% to by weight about 75%, by weight about Between 25% to by weight about 80%, between by weight about 25% to by weight about 85%, by weight about Between 25% to by weight about 90%, between by weight about 25% to by weight about 95%, by weight about Between 25% to by weight about 30%, between by weight about 25% to by weight about 35%, by weight about Between 25% to by weight about 40%, between by weight about 25% to by weight about 45%, by weight about Between 25% to by weight about 50%, between by weight about 25% to by weight about 55%, by weight about Between 25% to by weight about 60%, between by weight about 25% to by weight about 65%, by weight about Between 25% to by weight about 70%, between by weight about 25% to by weight about 75%, by weight about Between 25% to by weight about 80%, between by weight about 25% to by weight about 85%, by weight about Between 25% to by weight about 90%, between by weight about 25% to by weight about 95%, by weight about Between 30% to by weight about 35%, between by weight about 30% to by weight about 40%, by weight about Between 30% to by weight about 45%, between by weight about 30% to by weight about 50%, by weight about Between 30% to by weight about 55%, between by weight about 30% to by weight about 60%, by weight about Between 30% to by weight about 65%, between by weight about 30% to by weight about 70%, by weight about Between 30% to by weight about 75%, between by weight about 30% to by weight about 80%, by weight about Between 30% to by weight about 85%, between by weight about 30% to by weight about 90%, by weight about Between 30% to by weight about 95%, between by weight about 35% to by weight about 40%, by weight about Between 35% to by weight about 45%, between by weight about 35% to by weight about 50%, by weight about Between 35% to by weight about 55%, between by weight about 35% to by weight about 60%, by weight about Between 35% to by weight about 65%, between by weight about 35% to by weight about 70%, by weight about Between 35% to by weight about 75%, between by weight about 35% to by weight about 80%, by weight about Between 35% to by weight about 85%, between by weight about 35% to by weight about 90%, by weight about Between 35% to by weight about 95%, between by weight about 40% to by weight about 45%, by weight about Between 40% to by weight about 50%, between by weight about 40% to by weight about 55%, by weight about Between 40% to by weight about 60%, between by weight about 40% to by weight about 65%, by weight about Between 40% to by weight about 70%, between by weight about 40% to by weight about 75%, by weight about Between 40% to by weight about 80%, between by weight about 40% to by weight about 85%, by weight about Between 40% to by weight about 90%, between by weight about 40% to by weight about 95%, by weight about Between 45% to by weight about 50%, between by weight about 45% to by weight about 55%, by weight about Between 45% to by weight about 60%, between by weight about 45% to by weight about 65%, by weight about Between 45% to by weight about 70%, between by weight about 45% to by weight about 75%, by weight about Between 45% to by weight about 80%, between by weight about 45% to by weight about 85%, by weight about Between 45% to by weight about 90%, between by weight about 45% to by weight about 95%, by weight about Between 50% to by weight about 55%, between by weight about 50% to by weight about 60%, by weight about Between 50% to by weight about 65%, between by weight about 50% to by weight about 70%, by weight about Between 50% to by weight about 75%, between by weight about 50% to by weight about 80%, by weight about Between 50% to by weight about 85%, between by weight about 50% to by weight about 90%, by weight about Between 50% to by weight about 95%, between by weight about 55% to by weight about 60%, by weight about Between 55% to by weight about 65%, between by weight about 55% to by weight about 70%, by weight about Between 55% to by weight about 75%, between by weight about 55% to by weight about 80%, by weight about Between 55% to by weight about 85%, between by weight about 55% to by weight about 90%, by weight about Between 55% to by weight about 95%, between by weight about 60% to by weight about 65%, by weight about Between 60% to by weight about 70%, between by weight about 60% to by weight about 75%, by weight about Between 60% to by weight about 80%, between by weight about 60% to by weight about 85%, by weight about Between 60% to by weight about 90%, between by weight about 60% to by weight about 95%, by weight about Between 65% to by weight about 70%, between by weight about 65% to by weight about 75%, by weight about Between 65% to by weight about 80%, between by weight about 65% to by weight about 85%, by weight about Between 65% to by weight about 90%, between by weight about 65% to by weight about 95%, by weight about Between 70% to by weight about 75%, between by weight about 70% to by weight about 80%, by weight about Between 70% to by weight about 85%, between by weight about 70% to by weight about 90%, by weight about Between 70% to by weight about 95%, between by weight about 75% to by weight about 80%, by weight about Between 75% to by weight about 85%, between by weight about 75% to by weight about 90%, by weight about Between 75% to by weight about 95%, between by weight about 80% to by weight about 85%, by weight about Between 80% to by weight about 90%, between by weight about 80% to by weight about 95%, by weight about Between 85% to by weight about 90%, by weight about 85% to by weight about 95% or 90% to by weight about Between 95%.
The aspect of this specification partly discloses surfactant.Surfactant is the surface tension for reducing liquid Compound, allows easily to spread and reduce between two kinds of liquid or the interfacial tension between liquid and solid.Single table Face activating agent can be mixed with buffer solution disclosed herein or a variety of surfactants can be molten with buffering disclosed herein Liquid mixes.Useful surfactant include but not limited to ionic surfactant, amphoteric ion (both sexes) surfactant, Nonionic surface active agent or any combinations therein.The surfactant used in method disclosed herein can change, As understood by a person skilled in the art, and normally partially depend on the special buffer used, diluted protein and The conductivity value of use.
Ionic surfactant includes anionic surfactant.Anionic surfactant is included based on attached Be connected to the anionic surfactants of the permanent functional group on head (head) as such as sulfate, sulfonate, phosphate, Carboxylate or pH dependence anion type surfactants.Anionic surfactant includes but not limited to alkyl sulfate such as Ammonium lauryl sulfate and lauryl sodium sulfate (SDS)；Alkyl ether sulfate such as sodium laureth sulfate (sodium laureth sulfate) and myristyl alcohol polyethers sodium sulphate (sodium myreth sulfate)；More library esters (docusate) such as dioctyl sodium sulphosuccinate (dioctyl sodium sulfosuccinate)；Sulfonate fluorochemical surface Activating agent such as perfluoro octane sulfonate (PFOS) and perflurobutane sulfonate；Alkyl diphenyl ether dithionate such as DOWFAXTM 2A1 (two disodium sulfate of lauryl phenyl ether), DOWFAXTM3B2 (two disodium sulfate of decyl phenyl ether), DOWFAXTMC10L (decyls Two disodium sulfate of phenyl ether), DOWFAXTM2EP and DOWFAXTM8390 (two disodium sulfates of palmityl phenyl ether)；Potassium phosphate Polyether ester such as TRITONTMH-55 and TRITONTMH-66；Alkylbenzenesulfonate；Alkylaryl ether phosphate；Alkyl ether phosphates Salt；Alkyl carboxylate such as fatty acid salt and odium stearate；Sodium N-lauroyl sarcosinate；Carboxylate fluorine-containing surfactant such as perfluor Pelargonate and per-fluoro octanoate and hexyl diphenyl ether sodium sulphate (DOWFAXTM C6L)。
Ionic surfactant further includes cationic surface active agent.Cationic surface active agent is included based on forever The surfactant of long property cationic surfactant or pH dependence cationic surfactants, such as such as primary amine, secondary amine Or tertiary amine.Cationic surface active agent includes but not limited to alkyl trimethyl ammonium salt such as cetyltrimethylammonium bromide (CTAB) With cetyltrimethyl ammonium chloride (CTAC)；Chlorination palmityl pyridine (CPC)；The beef tallow amine (POEA) of polyethoxylated；Benzene Prick oronain (BAC)；Benzethonium chloride (BAC)；The bromo- 5- nitros -1,3- dioxanes of 5-；Dimethyldioctadecylammonium ammonium chloride；With it is double Primary amine, secondary amine or the tertiary amine of octadecyldimethyl ammonium bromide (DODAB) and pH dependences, such as wherein primary amine is more than 10 PH become the surfactant that positively charged or secondary amine becomes electrically charged lotus in the pH less than 4, such as dichloride Octenidine.Its He includes the anionic surfactant based on biology by useful anionic surfactant, including but not limited toAM 30-KE, Texapon Special andEHS, sodium 2-ethylhexyl sulfate.It is such Surfactant based on biology is not the molecule of synthesis, but the anionic surface derived from organic substance such as plant is lived Property agent.
Zwitterionic surfactant is based on primary amine, secondary amine or tertiary amine or quaternary ammonium cation and sulfate, carboxylate or phosphorus Hydrochlorate.Zwitterionic surfactant includes but not limited to 3- [(3- courages amidopropyl) dimethylammonio] -1- propane sulfonic acids Salt (CHAPS)；Sulfobetaines (sultaine) such as cocamidopropyl propyl amide hydroxyl sulfo betaine (cocamidopropyl hydroxysultaine)；Glycine betaine such as cocamidopropyl betaine；Or lecithin.
Nonionic surface active agent be less denaturation and therefore to dissolving memebrane protein and lipid be it is useful, together When retaining protein-protein interaction.Nonionic surface active agent includes polyethers nonionic surface active agent, poly- hydroxyl Base nonionic surface active agent and biosurfactant.Nonionic surface active agent includes alcohol ethoxylate, alkyl Phenol elhoxylate, phenol elhoxylate, acid amide ethoxylates, glycerol ethoxylate, fatty acid ethoxylate And amine ethoxylates.Nonionic surface active agent disclosed herein can have H (OCH2CH2)xOC6H4R1、 (OCH2CH2)xOR2Or H (OCH2CH2)xOC(O)R2General formula, wherein x represented added to alkylphenol and/or fatty alcohol or fat The molal quantity of ethylene oxide (ethylene oxide) in acid, R1Represent long chain alkyl group and R2Represent long-chain fat race Group.In in terms of this embodiment, R1It is C7-C10Alkyl group and/or R2It is C12-C20Aliphatic group.Other are useful Nonionic surface active agent include based on biology nonionic surface active agent, including but not limited toNonionic surface active agent derived from MET-10U molasses is undersaturated short-chain amides.It is such Surfactant based on biology is not the molecule of synthesis, but the non-ionic surface derived from organic substance such as plant is lived Property agent.
The non-limiting examples of surfactant include polyethylene glycol anhydrous sorbitol Arrcostab (or the sorb of ethoxylation Alcohol ester) as Polysorbate 20 dehydrating sorbitol monooleate (20), Polysorbate 40 anhydrosorbitol list oil Acid esters (40), Polysorbate 60 dehydrating sorbitol monooleate (60), 61 dehydration of polysorbate Sorbitol monooleate (61), Polysorbate 65 dehydrating sorbitol monooleate (65), gather 80 dehydrating sorbitol monooleate of sorbierite (80), 81 dehydrating sorbitol monooleate of polysorbate (81) and Polysorbate 85 dehydrating sorbitol monooleate (85)；Sorbitol ester such as dehydration Sorbitol monooleate, sorbitan monolaurate, sorbitan-monopalmityl ester, anhydrosorbitol list Stearate and anhydrosorbitol tristearate；Polyglycerol ester such as glyceryl monooleate, glyceryl monolaurate, Dan Zong Palmitic acid acid glyceride, glycerin monostearate, olein, castor-oil plant acid glyceride, glyceryl tristearate, single two glyceride And glyceryl triacetate；The polyglycerol ester of ethoxylation；Alkyl-glucoside such as peanut base glucoside (arachidyl glucoside)、C12-20Alkyl-glucoside, caprylyl/Octyl glucoside, cetearyl glucoside (cetearyl Glucoside), cocoyl glucoside, Ethyl-glucoside and lauryl glucoside, decyl glucoside；Ethyoxyl The alkyl-glucoside of change；Sucrose ester such as single sucrose oleate ester, mono laurate sucrose ester, single palmitic acid sucrose ester, monostearate Sucrose ester, three sucrose oleate esters, ricinoleic acid sucrose ester, three stearic acid sucrose esters, two glyceride of sucrose and triacetic acid sucrose ester； The sucrose ester of ethoxylation；Amine oxide；The alcohol of ethoxylation；The aliphatic alcohol of ethoxylation；Alkylamine；The alkane of ethoxylation Base amine；The nonyl phenol of the alkylphenol of ethoxylation such as ethoxylation and the octyl phenol of ethoxylation；Alkyl polysaccharide；Ethoxy The alkyl polysaccharide of base；The castor oil of the aliphatic acid of ethoxylation such as ethoxylation；Such as ethoxylation of the fatty alcohol of ethoxylation Cetyl oleyl alcohol, the cetyl stearyl alcohol of ethoxylation, the decyl alcohol of ethoxylation, ethoxylation lauryl alcohol and ethyoxyl The tridecanol of change；The fatty amine of ethoxylation；Pa network sand nurse class (polyethylene-polypropylene copolymer), as pa network sand nurse 124 (L44), pa network sand nurse 181 (L61), pa network sand nurse 182 ( L62), pa network sand nurse 184 (L64), pa network sand nurse 188 (F68), pa network sand nurse 237 (F87), pa network sand nurse 338 (L108) and pa network sand nurse 407 ( F127)；Linear secondary alcohol ethoxylate such as TERGITOLTM 15-S-5、TERGITOLTM 15-S-7、TERGITOLTM 15-S- 9、TERGITOLTM 15-S-12、TERGITOLTM 15-S-15、TERGITOLTM 15-S-20、TERGITOLTM15-S-30 with And TERGITOLTM15-S-40；Alkylphenol polyol ethers；Alkyl aryl polyethyleneglycol ether；Polyethylene glycol alkyl ether, such as eight second Glycol list dodecyl ether, five ethylene glycol list dodecyl ether,30 and35；12 ethoxy-ethanols of 2- (-PX)；Polyethylene glycol octyl phenol ether such as polyethylene glycol (4-5) to tert-octyl phenol (X-45) With Triton X-100 (X-100)；Polyethylene glycol alkyl phenols ether such as Nonoxynol-9；Phenoxy group gathers Ethoxy ethanol such as nonylphenoxy polyethoxy ethyl alcohol and octylphenoxy polyethoxy ethanol (CA-630 or NONIDETTMP-40)；Glucoside alkyl ether such as octylglucopyranoside glycosides；Maltoside alkyl ether such as dodecyl pyrans wheat Bud glucosides；Thioglucoside alkyl ether such as heptyl pyrans thioglucoside；Cardigin, alkyl esters of glycerol such as lauric acid Ester；Alkyl aryl polyether sulfuric ester；Sulfonic acid alcohol ester；Anhydrosorbitol Arrcostab；Coconut oleoyl amine ethanol amine such as coconut oleoyl amine one Ethanol amine and cocamide diethanolamine；Mono laurate sucrose ester；Dodecyl dimethylamine oxide and sodium taurocholate.Herein In disclosed method other non-limiting examples of useful surfactant can in such as Winslow, et al., Methods and Compositions for Simultaneously Isolating Hemoglobin from Red Blood Cells and Inactivating Viruses,U.S.2008/0138790；Pharmaceutical Dosage Forms and Drug Delivery Systems (Howard C.Ansel, et al., editor, Lippincott Williams＆Wilkins Publishers, the 7th edition, 1999)；Remington:The Science and Practice of Pharmacy(Alfonso R.Gennaro is edited, Lippincott, Williams＆Wilkins, the 20th edition, and 2000)；Goodman&Gilman's The Pharmacological Basis of Therapeutics (Joel G.Hardman et al., editor, McGraw-Hill Professional, the 10th edition, 2001) and Handbook of Pharmaceutical Excipients (Raymond C.Rowe, et al., APhA Publications, the 4th edition, 2003) in be found, each in these bibliography being led to accordingly Reference is crossed to be integrally incorporated with it.
Nonionic surface active agent synergistically works with the effect for enhancing the microorganism supernatant to ferment.In addition, It has been determined that the nonionic surface active agent used in plant agent composition disclosed herein is with enhancing chemical action Compatible.Therefore, in embodiments, plant agent composition disclosed herein is only comprising one or more of non-ionic surfaces Activating agent.In another embodiment, plant agent composition disclosed herein is only comprising one or more of non-ionic tables Face activating agent and one or more of anionic surfactant.In another embodiment, plant agent disclosed herein Composition does not include any cationic surface active agent.In another embodiment, plant agent composition disclosed herein Not comprising any cationic surface active agent or zwitterionic surfactant.In another embodiment, it is disclosed herein Plant agent composition do not include any ionic surfactant.In another embodiment, plant agent disclosed herein Composition does not include any ionic surfactant or zwitterionic surfactant.
The surfactant disclosed herein of any amount can be used, condition is that the amount is to putting into practice method disclosed herein Useful.In in terms of this embodiment, the amount of the surfactant used is such as by weight about 0.01%, by weight Meter about 0.05%, by weight about 0.075%, by weight about 0.1%, by weight about 0.2%, by weight about 0.3%, By weight about 0.4%, by weight about 0.5%, by weight about 0.6%, by weight about 0.7%, by weight about 0.8%th, by weight about 0.9%, by weight about 1.0%, by weight about 1.5%, by weight about 2.0%, by weight Meter about 2.5%, by weight about 3.0%, by weight about 4.0%, by weight about 5.0%, by weight about 6.0%, press Weight meter about 7.0%, by weight about 7.5%, by weight about 8.0%, by weight about 9.0% or by weight about 10.0%.In in terms of other of this embodiment, the amount of the surfactant used is such as by weight at least 0.01%, By weight at least 0.05%, by weight at least 0.075%, by weight at least 0.1%, by weight at least 0.25%, By weight at least 0.5%, by weight at least 0.75%, by weight at least 1.0%, by weight at least 1.5%, by weight Gauge at least 2.0%, by weight at least 2.5%, by weight at least 3.0%, by weight at least 4.0%, by weight At least 5.0%, by weight at least 6.0%, by weight at least 7.0%, by weight at least 7.5%, by weight at least 8.0%th, by weight at least 9.0% or by weight at least 10.0%.This embodiment again in terms of other in, use The amount of surfactant is such as by weight at most 0.01%, by weight at most 0.05%, by weight at most 0.075%th, by weight at most 0.1%, by weight at most 0.25%, by weight at most 0.5%, by weight at most 0.75%th, by weight at most 1.0%, by weight at most 1.5%, by weight at most 2.0%, by weight at most 2.5%th, by weight at most 3.0%, by weight at most 4.0%, by weight at most 5.0%, by weight at most 6.0%th, by weight at most 7.5%, by weight at most 8.0%, by weight at most 9.0% or by weight at most 10.0%.
This embodiment it is other in terms of other in, the amount of the surfactant used is for example by weight about Between 0.1% to by weight about 0.5%, between by weight about 0.1% to by weight about 0.75%, by weight Between about 0.1% to by weight about 1.0%, between by weight about 0.1% to by weight about 1.5%, by weight Meter about 0.1% to by weight about 2.0% between, between by weight about 0.1% to by weight about 2.5%, by weight Between gauge about 0.2% to by weight about 0.5%, between by weight about 0.2% to by weight about 0.75%, by Between weight meter about 0.2% to by weight about 1.0%, between by weight about 0.2% to by weight about 1.5%, Between by weight about 0.2% to by weight about 2.0%, between by weight about 0.2% to by weight about 2.5%, Between by weight about 0.5% to by weight about 1.0%, by weight about 0.5% to by weight about 1.5% it Between, between by weight about 0.5% to by weight about 2.0%, by weight about 0.5% to by weight about 2.5% Between, between by weight about 0.5% to by weight about 3.0%, by weight about 0.5% to by weight about Between 4.0%, between by weight about 0.5% to by weight about 5.0%, by weight about 1.0% to by weight Between about 2.5%, between by weight about 1.0% to by weight about 3.0%, by weight about 1.0% to by weight Meter about 4.0% between, between by weight about 1.0% to by weight about 5.0%, by weight about 1.0% to by weight Between gauge about 6.0%, between by weight about 1.0% to by weight about 7.0%, by weight about 1.0% to by Between weight meter about 7.5%, between by weight about 1.0% to by weight about 8.0%, by weight about 1.0% to Between by weight about 9.0%, between by weight about 1.0% to by weight about 10.0%, by weight about 2.0% Between to by weight about 2.5%, between by weight about 2.0% to by weight about 3.0%, by weight about Between 2.0% to by weight about 4.0%, between by weight about 2.0% to by weight about 5.0%, by weight Between about 2.0% to by weight about 6.0%, between by weight about 2.0% to by weight about 7.0%, by weight Meter about 2.0% to by weight about 7.5% between, between by weight about 2.0% to by weight about 8.0%, by weight Between gauge about 2.0% to by weight about 9.0%, between by weight about 2.0% to by weight about 10.0%, by Between weight meter about 5.0% to by weight about 6.0%, between by weight about 5.0% to by weight about 7.0%, Between by weight about 5.0% to by weight about 7.5%, between by weight about 5.0% to by weight about 8.0%, Between by weight about 5.0% to by weight about 9.0%, by weight about 5.0% to by weight about 10.0% it Between, between by weight about 5.0% to by weight about 11.0%, by weight about 5.0% to by weight about Between 12.0%, between by weight about 5.0% to by weight about 13.0%, by weight about 5.0% to by weight Between meter about 14.0% or between by weight about 5.0% to by weight about 15.0%.
The aspect of this specification partly discloses the pH of plant agent composition disclosed herein.Plant agent composition is most Whole pH is typically acid, because this contributes to the longer storage period of composition.In in terms of this embodiment, herein The pH of disclosed plant agent composition is for example, about 2, about 2.5, about 3, about 3.5, about 4, about 4.5, about 5, about 5.5 or about 6.Herein In other aspects of embodiment, the pH of plant agent composition disclosed herein is for example, at least 2, at least 2.5, at least 3, at least 3.5th, at least 4, at least 4.5, at least 5, at least 5.5 or at least 6.This embodiment again in terms of other in, it is disclosed herein The pH of plant agent composition is such as at most 2, at most 2.5, at most 3, at most 3.5, at most 4, at most 4.5, at most 5, at most 5.5 Or to how many 6.This embodiment it is other in terms of other in, the pH of plant agent composition disclosed herein for example about 2 to Between about 3, between about 2 to about 3.5, between about 2 to about 4, between about 2 to about 4.5, between about 2 to about 5, about 2 Between to about 5.5, between about 2 to about 6, between about 2.5 to about 3, between about 2.5 to about 3.5, about 2.5 to about 4 it Between, between about 2.5 to about 4.5, between about 2.5 to about 5, between about 2.5 to about 5.5, between about 2.5 to about 6, Between about 3 to about 3.5, between about 3 to about 4, between about 3 to about 4.2, between about 3 to about 4.5, about 3 to about 4.7 Between, between about 3 to about 5, between about 3 to about 5.2, between about 3 to about 5.5, between about 3 to about 6, about 3.5 Between to about 4, between about 3.5 to about 4.2, between about 3.5 to about 4.5, between about 3.5 to about 4.7, about 3.5 to Between about 5, between about 3.5 to about 5.2, between about 3.5 to about 5.5, between about 3.5 to about 6, about 3.7 to about 4.0 Between, between about 3.7 to about 4.2, between about 3.7 to about 4.5, between about 3.7 to about 5.2, about 3.7 to about 5.5 Between or between about 3.7 to about 6.0.
Mammal, plant life and environment of the plant agent composition disclosed herein to the mankind, including domestic animal have most Small side effect.In in terms of this embodiment, plant agent composition disclosed herein to the mankind, mammal, plant and Environment is generally nontoxic.In in terms of other of this embodiment, plant agent composition disclosed herein is to the mankind, lactation Animal, plant and environment are substantially nontoxic.
The aspect of this specification partly discloses plant agent composition, and the plant agent composition is biodegradable 's.Biodegradable plant agent composition disclosed herein is once by after application according to method disclosed herein and purposes Make it easy to degrade, corrode, reabsorb, decomposing (decompose) or destroy (break down) into essence or obvious degree can Biodegradable plant agent composition.In in terms of this embodiment, at least 75%, at least 80%, at least 85%, at least 90%th, at least 95%, at least 99% plant agent composition disclosed herein for example, about 1 day, about 2 days, about 3 days, about 4 days, It is biodegradable in about 5 days, about 6 days or about 7 days.In in terms of other of this embodiment, at least 75%, at least 80%, at least 85%th, at least 90%, at least 95%, at least 99% plant agent composition disclosed herein for example, about 1 day to about 2 days, about 1 It to about 3 days, about 1 day to about 4 days, about 1 day to about 5 days, about 1 day to about 6 days, about 1 day to about 7 days, about 2 days to about 3 days, about 2 days to about 4 days, about 2 days to about 5 days, about 2 days to about 6 days, about 2 days to about 7 days, about 3 days to about 4 days, about 3 days to about 5 days, About 3 days to about 6 days, about 3 days to about 7 days, about 4 days to about 5 days, about 4 days to about 6 days, about 4 days to about 7 days, about 5 days to about 6 My god, it is biodegradable in about 5 days to about 7 days or about 6 days to about 7 days.
In in terms of this embodiment, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, extremely Few 99% plant agent composition disclosed herein for example, about 7 days, about 8 days, about 9 days, about 10 days, about 11 days, about 12 days, about It is biodegradable in 13 days or about 14 days.In in terms of other of this embodiment, at least 75%, at least 80%, at least 85%, extremely Few 90%, at least 95%, at least 99% plant agent composition disclosed herein was at for example, about 7 days to about 8 days, about 7 days to about 9 My god, about 7 days to about 10 days, about 7 days to about 11 days, about 7 days to about 12 days, about 7 days to about 13 days, about 7 days to about 14 days, about 8 It was to about 9 days, about 8 days to about 10 days, about 8 days to about 11 days, about 8 days to about 12 days, about 8 days to about 13 days, about 8 days to about 14 My god, about 9 days to about 10 days, about 9 days to about 11 days, about 9 days to about 12 days, about 9 days to about 13 days, about 9 days to about 14 days, about 9 It to about 11 days, about 9 days to about 12 days, about 9 days to about 13 days, about 9 days to about 14 days, about 10 days to about 11 days, about 10 days extremely About 12 days, about 10 days to about 13 days, about 10 days to about 14 days, about 11 days to about 12 days, about 11 days to about 13 days, about 11 days to about It is biodegradable in 14 days, about 12 days to about 13 days, about 12 days to about 14 days or about 13 days to about 14 days.
In in terms of this embodiment, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, extremely Few 99% plant agent composition disclosed herein was at for example, about 15 days, about 16 days, about 17 days, about 18 days, about 19 days, about 20 days It is or biodegradable in about 21 days.In in terms of other of this embodiment, at least 75%, at least 80%, at least 85%, at least 90%th, at least 95%, at least 99% plant agent composition disclosed herein for example, about 15 days to about 16 days, about 15 days to about 17 days, about 15 days to about 18 days, about 15 days to about 19 days, about 15 days to about 20 days, about 15 days to about 21 days, about 16 days to about 17 My god, about 16 days to about 18 days, about 16 days to about 19 days, about 16 days to about 20 days, about 16 days to about 21 days, about 17 days to about 18 My god, about 17 days to about 19 days, about 17 days to about 20 days, about 17 days to about 21 days, about 18 days to about 19 days, about 18 days to about 20 My god, about 18 days to about 21 days, about 19 days to about 20 days, it is biodegradable in about 19 days to about 21 days or about 20 days to about 21 days.
The aspect of this specification partly discloses kit, and the kit is included for putting into practice method disclosed herein Or one or more of components (component) that purposes is useful.Kit provide can be used for put into practice method disclosed herein or The convenient shell (enclosure) of the component of purposes is to promote or enhance commercial distribution.For example, kit can include herein Disclosed plant agent composition and other one or more of reagents available for putting into practice method disclosed herein or purposes, such as Such as one or more of diluents and/or one or more of carriers.
Kit typically provides suitable container, such as comprising available for putting into practice the one of method disclosed herein or purposes The box of kind or more kind component or carriers of other closings.In addition, kit disclosed herein will typically comprise individual appearance Device, such as bottle, bottle (vial), flask or the carrier of other closings comprising one or more of components.For example, for herein It the container of disclosed plant agent composition and is included in kit for the individual container of one or more of reagents.Examination Agent box can be portable, such as can be transported and make in such as business or the remote districts in industrial equipment or farmland With.Other kits can have purposes in residential housing.
Kit disclosed herein can include label or insert.Label or insert include that individual material can be used as Material, packaging material (such as box) are provided or are attached or be fixed to " printed matter " of the container comprising reagent constituents.Mark Label or insert can also comprise：Computer-readable medium, such as disk (such as hard disk, flash memory)；CD such as CD or DVD-ROM/RAM, DVD, MP3, tape；Or electric storage medium such as RAM and ROM；Or these mixing, such as magnetism/optics Storage medium, FLASH media or type of memory card.Label or insert can include determining that one or more of components therein Information, dosage, dose frequency or timing, the information about individual component.Label or insert can include determining that manufacture Quotient's information, article No., manufacturer position and the information on date.Label or insert can include about reagent constituents can by with In condition or situation information.Label or insert can include being used for the examination using in method as disclosed herein or purposes One or more of specifications in agent box component.Specification can include dosage, frequency or duration and for putting into practice The explanation of any method or purposes or processing scheme described herein and about potential hazard or wherein by inappropriate using trying The warning of situation in the case of agent box component.
The method that the aspect of this specification partly discloses the virulence factor of control plant disease.Disclosed control plant The method of the virulence factor of disease includes the following steps：A effective amount of plant agent composition disclosed herein, which is applied to infect, to be had One or more of plants of virulence factor and/or will have in a manner that wherein virulence factor will be exposed to plant agent composition The plant agent composition disclosed herein of effect amount is applied to one or more places.Plant agent composition disclosed herein is answered With the adverse effect caused for example to the virulence factor for seeking controlled plant disease.
The aspect of this specification partly discloses plant agent composition disclosed herein for controlling the cause of plant disease The purposes of cause of disease.The purposes of disclosed plant agent composition is included a effective amount of plant agent composition application disclosed herein To infecting one or more of plants for having virulence factor and/or will be exposed to plant agent composition with wherein virulence factor A effective amount of plant agent composition disclosed herein is applied to one or more places by mode.Plant agent group disclosed herein The application of conjunction object leads to the adverse effect for example to the virulence factor for seeking controlled plant disease.
The aspect of this specification partly discloses the method for increasing plant growth and/or crop production.Disclosed increase The method of plant growth and/or crop production includes the following steps：By a effective amount of plant agent composition application disclosed herein To have to one or more of plants and/or in a manner that one of which or more kind plant will be exposed to plant agent composition The plant agent composition disclosed herein of effect amount is applied to one or more places.Plant agent composition disclosed herein is answered With causing for example by the improved absorption of root hair, improved xylem sap flowing by xylem and improved in bast In portion photosynthate flowing, the absorption of increased water, minerals and other nutrients from soil, it is increased The synthesis of capillarity and/or hydrostatic pressing and/or increased compound and energy in xylem and/or blocking xylem Juice flows and/or the destruction of one or more of components of photosynthate flowing.In embodiments, stop xylem Juice flows and/or one or more of components of photosynthate flowing include biomembrane.
The aspect of this specification partly disclose plant agent composition disclosed herein for increase plant growth and/or The purposes of crop production.The purposes of disclosed plant agent composition includes should by a effective amount of plant agent composition disclosed herein Wherein plant agent combination is applied to one or more of plants and/or by a effective amount of plant agent composition disclosed herein One or more places that object will be exposed to one or more of plants.The application of plant agent composition disclosed herein is led It causes for example by the improved absorption of root hair, improved xylem sap flowing by xylem and improved in bast Photosynthate flowing, the absorption of increased water, minerals and other nutrients from soil is increased wooden The synthesis of capillarity and/or hydrostatic pressing and/or increased compound and energy in portion and/or blocking xylem sap The destruction of one or more of components of flowing and/or photosynthate flowing.In embodiments, stop xylem sap One or more of components of flowing and/or photosynthate flowing include biomembrane.
The aspect of this specification partly discloses the method for efficiency for keeping or improving the irrigation system.It is disclosed holding or The method of the efficiency to improve the irrigation system includes the following steps：A effective amount of plant agent composition disclosed herein is applied to filling Irrigate one or more pipelines in the pipeline network of system.The application of plant agent composition disclosed herein causes for example to stop The suitable removing of one or more of components of one or more pipeline networks of irrigation system.In embodiments, it hinders The one or more of components for keeping off one or more pipeline networks include biomembrane.
The aspect of this specification partly discloses plant agent composition disclosed herein and irrigates system for keeping or improving The purposes of the efficiency of system.The purposes of disclosed plant agent composition includes：By a effective amount of plant agent composition disclosed herein One or more pipelines being applied in the pipeline network of irrigation system.The application of plant agent composition disclosed herein causes Such as the suitable removing of one or more of components of one or more pipeline networks of blocking irrigation system.In embodiment party In case, stop that one or more of components of one or more pipeline networks include biomembrane.
On the microorganism of nonionic surface active agent and the processed fermentation in plant agent composition disclosed herein The combination of clear liquid leads to the acceleration of chemical bond present in following molecular structure, particularly polysaccharide and component based on lipid In-situ chemical reaction：1) one or more of components present in the protective structures of the virulence factor of plant disease；2) stop Xylem sap and/or one or more of components of photosynthate flowing in plant；Or 3) stop water in irrigation system One or more of components of flowing.The dissolving of these in-situ chemical reactions, dispersion otherwise destroy：1) virulence factor Protective structures one or more of components, by destroy one or more of basic physiological processes cause it is described it is pathogenic because The death of son；2) stop one kind or more that photosynthate flows in xylem sap flowing in xylem and/or bast Various ingredients cause the improved transport of water and nutrients, water and nutrients that will keep and/or enhance health and the work of plant Power；Or 3) one or more of components of water flowing in the pipeline network of irrigation system are destroyed, lead to improved water distribution, this changes Into water distribution will keep and/or enhancing plant health and vigor.
It does not wish to be bound by any theory restrictions, in aqueous environment after appliable plant agent composition, spontaneously forms height Reactive, unique texture, ultra-fine microbubble.These " functionalized " microbubbles include external " highly reactive " shell and Inner core, the shell include one or more of nonionic surface active agent and the microorganism supernatant from processed fermentation The component of liquid, the inner core include air." highly reactive " shell makes it possible to realize the quality of the oxygen in aqueous environment The violent living things catalysis increased with the acceleration of the molecular structure of compound transmitted, provides synergistic function to a combination thereof.About oxygen Mass transfer, this functional level for increasing the delivery rate of oxygen and improve the dissolved oxygen in aqueous environment, level is far More than the solubility limit as expected from Henry's law and in the level that cannot be realized simply by mechanical ventilation system.Seem The component interference nonionic surface active agent of microorganism supernatant from processed fermentation, which generates, assembles good micella The ability of shell.The result is that the loose packing of molecules of these fermentation components and surfactant, makes shell " functionalization " with more ventilative, More favorable condition is created so as to be transmitted for a large amount of gases.Therefore, what this oxygen transfer function increased the oxygen in aqueous environment can The property used.About the living things catalysis of acceleration, this functionality reacts platform to reduce the energy needed for catalysis reaction generation by offer The transformation of amount, the reaction platform increase the local concentration of reactant, enabling contribute electronics and promote in the bad position of electronics The chemical reaction of point.Therefore, the fracture of chemical bond present in this biocatalytic Activity mediation compound, the chemical bond include Glycosidic bond and ester bond.Therefore, " functionalized " shell of microbubble has the catalytic activity similar to conventional enzyme system, but does not need to Any enzyme.Therefore, " functionalized " microbubble is generated using plant agent composition disclosed herein, it is described " functionalized：Micro- gas Bubble increases the interaction of molecules that the oxygen of higher dissolved oxygen levels is caused to dissipate and accelerates the catalytic decomposition for leading to compound.
When the protective structures with virulence factor contact, " functionalized " microbubble is can contribute electronics or in electronics At bad site react mode and protective structures one or more of component chemicals interact, the mode mediates The fracture of chemical bond present in one or more of components, the chemical bond include glycosidic bond and ester bond.Similarly, when with resistance When the xylem sap and/or one or more of components of photosynthate flowing kept off in plant contacts, " functionalized " Microbubble is with one or more of component chemicals mutual in a manner of it can contribute electronics or be reacted at the bad site of electronics Effect, the mode mediate the fracture of chemical bond present in one or more of components, the chemical bond include glycosidic bond and Ester bond.Similarly, when one or more of components with water flowing in blocking irrigation system contact, " functionalized " microbubble It is interacted in a manner of it can contribute electronics or be reacted at the bad site of electronics with one or more of component chemicals, institute The mode of stating mediates the fracture of chemical bond present in one or more of components, and the chemical bond includes glycosidic bond and ester bond.This A little interactions seem the hydrolysed form using beta oxidation, wherein other than depending on " highly reactive property " shell, also using micro- Oxygen present in the core of bubble.Therefore, the oxygen transfer ability of property and core present in " functionalized " microbubble synergistically rises Effect is destroyed with enhancing the in situ of chemical bond, the chemical bond include it is following present in glycosidic bond and ester bond：1) plant disease Virulence factor protective structures present in one or more of components；2) stop xylem sap and/or light in plant One or more of components that cooperation product flows；And/or 3) stop one or more of groups of water flowing in irrigation system Point.
In addition, when being contacted with root hair, Water transport is more permeated by the film of root hair and to enhance root hair function Symbiont provides better microbial environment, and " functionalized " microbubble increases water and absorbs, and increases nitrogen and fixes, increases gas It exchanges, increases capillarity and hydrostatic pressing in vascular tissue.Such interaction improves the absorption by root hair, improves It is flowed by the xylem sap of xylem and improves the photosynthate in bast and flowed, this causes will be for keeping And/or enhance the improved transport of the health of the plant and raw material of vigor, growth components and energy.
The application of plant agent composition disclosed herein can be causing a disease plant disease by following any method One or more of components present in the protective structures of the factor are exposed to disclosed plant agent composition, and mode is to pass through Destroy one or more of basic physiological processes provide protective structures one or more of components it is suitable destroy and with Death afterwards.For example, exposure can be by being applied directly to virulence factor or will be by by indirect application to wherein virulence factor It is exposed to the place of plant agent composition.
Similarly, the application of plant agent composition disclosed herein can pass through following any method：To provide increase The absorption of water, minerals and other nutrients from soil, increase in xylem capillarity and/or hydrostatic pressing and/ Or increase the synthesis of compound and energy and root hair absorb, pass through xylem xylem sap flowing and bast in photosynthetic work Root hair is exposed to disclosed plant agent composition by the subsequent improved mode flowed with product.For example, exposure can be by straight It connects to be applied to one or more of plants or plant plant by indirect application to one of which or more and will be exposed to plant agent The place of composition.
Similarly, the application of plant agent composition disclosed herein can pass through following any method：To provide protection Property structure the suitable of one or more of components destroy and will keep and/or the Shui Heying of the health of enhancing plant and vigor The subsequent improved mode of the transport of object is supported, by photosynthesis in xylem sap flowing in blocking xylem and/or bast One or more of components of product flowing are exposed to disclosed plant agent composition.For example, exposure can be by directly applying Plant agent composition will be exposed to by one or more of plants or planting plant by indirect application to one of which or more Place.
Similarly, the application of plant agent composition disclosed herein can pass through following any method：To provide protection Property structure the suitable of one or more of components destroy and will keep and/or the irrigation system of the health of enhancing plant and vigor The subsequent improved mode of water distribution in system, by the one or more of the water flowing in the pipeline network for destroying irrigation system Component is exposed to disclosed plant agent composition.For example, exposure can be by being applied directly to the one or more of irrigation system A pipeline network will be exposed to plant agent by one or more pipeline networks of indirect application to wherein irrigation system The place of composition.
The undiluted form of plant agent composition disclosed herein can use in method disclosed herein and purposes. Selectively, plant agent composition disclosed herein can be desirably diluted, and those skilled in the art will know that can be made With the dilution (dilution) of such composition.The dilution of plant agent composition disclosed herein can typically use water It carries out, although other suitable diluents can also be used, as long as the formation of they and microbubble as disclosed herein is phase Hold.In in terms of this embodiment, plant agent composition is diluted into following ratio：Such as 1:10、1:25、1:50、 1:75、1:100、1:200、1:300、1:400、1:500、1:600、1:700、1:800、1:900、1:1000、1:2000、1: 3000、1:4000、1:5000、1:6000、1:7000、1:8000、1:9000、1:10000、1:20000、1:30000、1: 4000、1:50000、1:60000、1:70000、1:80000、1:90000 or 1:100000.In terms of other of this embodiment In, plant agent composition is diluted into following ratio：For example, at least 1:10th, at least 1:25th, at least 1:50th, at least 1:75th, extremely Few 1:100th, at least 1:200th, at least 1:300th, at least 1:400th, at least 1:500th, at least 1:600th, at least 1:700th, at least 1: 800th, at least 1:900th, at least 1:1000th, at least 1:2000th, at least 1:3000th, at least 1:4000th, at least 1:5000th, at least 1: 6000th, at least 1:7000th, at least 1:8000th, at least 1:9000th, at least 1:10000th, at least 1:20000th, at least 1:30000th, extremely Few 1:4000th, at least 1:50000th, at least 1:60000th, at least 1:70000th, at least 1:80000th, at least 1:90000 or at least 1: 100000.This embodiment again in terms of other in, plant agent composition is diluted into following ratio：Such as at most 1: 10th, at most 1:25th, at most 1:50th, at most 1:75th, at most 1:100th, at most 1:200th, at most 1:300th, at most 1:400th, at most 1: 500th, at most 1:600th, at most 1:700th, at most 1:800th, at most 1:900th, at most 1:1000th, at most 1:2000th, at most 1:3000、 At most 1:4000th, at most 1:5000th, at most 1:6000th, at most 1:7000th, at most 1:8000th, at most 1:9000th, at most 1: 10000th, at most 1:20000th, at most 1:30000th, at most 1:4000th, at most 1:50000th, at most 1:60000th, at most 1:70000、 At most 1:80000th, at most 1:90000 or at most 1:100000.
In the other in terms of other of this embodiment, plant agent composition is diluted into following ratio：For example, about 1:1 to About 1:10th, about 1:1 to about 1:25th, about 1:1 to about 1:50th, about 1:1 to about 1:75th, about 1:1 to about 1:100th, about 1:2 to about 1: 10th, about 1:2 to about 1:25th, about 1:2 to about 1:50th, about 1:2 to about 1:75th, about 1:2 to about 1:100th, about 1:10 to about 1:25th, about 1:10 to about 1:50th, about 1:10 to about 1:75th, about 1:10 to about 1:100th, about 1:10 to about 1:125th, about 1:10 to about 1:150、 About 1:10 to about 1:175th, about 1:10 to about 1:200th, about 1:10 to about 1:225th, about 1:10 to about 1:250th, about 1:50 to about 1: 100th, about 1:50 to about 1:200th, about 1:50 to about 1:300th, about 1:50 to about 1:400th, about 1:50 to about 1:500th, about 1:50 to About 1:600th, about 1:50 to about 1:700th, about 1:50 to about 1:800th, about 1:50 to about 1:900th, about 1:50 to about 1:1000th, about 1: 100 to about 1:200th, about 1:100 to about 1:300th, about 1:100 to about 1:400th, about 1:100 to about 1:500th, about 1:100 to about 1: 600th, about 1:100 to about 1:700th, about 1:100 to about 1:800th, about 1:100 to about 1:900th, about 1:100 to about 1:1000th, about 1: 500 to about 1:1000th, about 1:500 to about 1:2000th, about 1:500 to about 1:3000th, about 1:500 to about 1:4000th, about 1:500 to About 1:5000th, about 1:500 to about 1:6000th, about 1:500 to about 1:7000th, about 1:500 to about 1:8000th, about 1:500 to about 1: 9000th, about 1:500 to about 1:10000th, about 1:1000 to about 1:2000th, about 1:1000 to about 1:3000th, about 1:1000 to about 1: 4000th, about 1:1000 to about 1:5000th, about 1:1000 to about 1:6000th, about 1:1000 to about 1:7000th, about 1:1000 to about 1: 8000th, about 1:1000 to about 1:9000th, about 1:1000 to about 1:10000th, about 1:5000 to about 1:10000th, about 1:5000 to about 1:20000th, about 1:5000 to about 1:30000th, about 1:5000 to about 1:40000th, about 1:5000 to about 1:50000th, about 1:5000 To about 1:60000th, about 1:5000 to about 1:70000th, about 1:5000 to about 1:80000th, about 1:5000 to about 1:90000th, about 1: 5000 to about 1:100000th, about 1:10000 to about 1:20000th, about 1:10000 to about 1:30000th, about 1:10000 to about 1: 40000th, about 1:10000 to about 1:50000th, about 1:10000 to about 1:60000th, about 1:10000 to about 1:70000th, about 1: 10000 to about 1:80000th, about 1:10000 to about 1:90000th, about 1:10000 to about 1:100000.
The application of plant agent composition disclosed herein is with effective quantity.The effective quantity of disclosed plant agent composition can be with It is：1) it is enough the amount that the group of the virulence factor to seeking controlled plant disease adversely affects；2) it is enough to improve logical The absorption of root hair is crossed, improves and flows and improve what photosynthate in bast flowed by the xylem sap of xylem Amount；3) it is enough to increase the absorption of water, minerals and other nutrients from soil, increases capillarity in xylem And/or the amount of the synthesis of hydrostatic pressing and/or increase compound and energy；4) it is enough to cause blocking xylem sap and/or photosynthetic Act on the amount suitably destroyed of one or more of components of product flowing；And/or 5) it is enough to cause blocking irrigation system The amount suitably removed of one or more of components of one or more pipeline networks.The reality of disclosed plant agent composition Border effective quantity is by being used to assess the control activity of plant agent composition disclosed herein and the conventional screening sequence of efficiency come really It is fixed.Such screening sequence is well known to those skilled in the art., it is expected that the plant disclosed herein with higher activity level Agent composition can be used with smaller amount and concentration, and the plant agent composition with relatively low activity level may need Larger quantities or concentration are wanted to realize identical control effect.
The application of plant agent composition disclosed herein is with effective quantity.The effective quantity of disclosed plant agent composition can be with It is the amount for being enough to cause desired effect herein.The actually active amount of disclosed plant agent composition is public herein by being used to assess The control activity for the plant agent composition opened is determined with the conventional screening sequence of efficiency.Such screening sequence is this field skill Known to art personnel., it is expected that the plant agent composition disclosed herein with higher activity level can with smaller amount and Concentration uses, and the plant agent composition with relatively low activity level may need larger quantities or concentration to realize phase Same control effect.
The effective quantity of disclosed plant agent composition can be enough to cause unfavorable shadow to seeking controlled virulence factor Loud amount.In in terms of this embodiment, the effective quantity of disclosed plant agent composition is the group being enough to infecting plant In the amount that adversely affects of following virulence factor：For example, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%th, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90% or about 95%.In in terms of other of this embodiment, the effective quantity of disclosed plant agent composition is enough to infecting The amount that following virulence factor in the group of plant adversely affects：For example, at least 10%, at least 15%, at least 20%, At least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%th, at least 70%, at least 75%, at least 80%, at least 85%, at least 90% or at least 95%.In the another of this embodiment In other outer aspects, the effective quantity of disclosed plant agent composition be enough to infect plant group in it is following it is pathogenic because The amount that son adversely affects：Such as at most 10%, at most 15%, at most 20%, at most 25%, at most 30%, at most 35%, At most 40%, at most 45%, at most 50%, at most 55%, at most 60%, at most 65%, at most 70%, at most 75%, at most 80%th, at most 85%, at most 90% or at most 95%.This embodiment again in terms of other in, disclosed plant agent combination The effective quantity of object be enough to infect plant group in the amount that adversely affects of following virulence factor：For example, about 10% To about 20%, about 10% to about 30%, about 10% to about 40%, about 10% to about 50%, about 10% to about 60%, about 10% to About 70%, about 10% to about 80%, about 10% to about 90%, about 10% to about 95%, about 20% to about 30%, about 20% to about 40%th, about 20% to about 50%, about 20% to about 60%, about 20% to about 70%, about 20% to about 80%, about 20% to about 90%th, about 20% to about 95%, about 30% to about 40%, about 30% to about 50%, about 30% to about 60%, about 30% to about 70%th, about 30% to about 80%, about 30% to about 90%, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%th, about 40% to about 70%, about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%th, about 50% to about 70%, about 50% to about 80%, about 50% to about 90%, about 50% to about 95%, about 60% to about 70%th, about 60% to about 80%, about 60% to about 90%, about 60% to about 95%, about 70% to about 80%, about 70% to about 90%th, about 70% to about 95%, about 80% to about 90%, about 80% to about 95% or about 90% to about 95%.
In in terms of this embodiment, the effective quantity of disclosed plant agent composition is the group being enough to infecting plant In the murderous amount of following virulence factor：For example, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, About 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90% Or about 95%.In in terms of other of this embodiment, the effective quantity of disclosed plant agent composition is enough to infecting plant Group in the murderous amount of following virulence factor：For example, at least 10%, at least 15%, at least 20%, at least 25%, At least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%th, at least 75%, at least 80%, at least 85%, at least 90% or at least 95%.In this embodiment again in terms of other In, the effective quantity of disclosed plant agent composition be enough to infect plant group in following virulence factor cause death Amount：Such as at most 10%, at most 15%, at most 20%, at most 25%, at most 30%, at most 35%, at most 40%, at most 45%th, at most 50%, at most 55%, at most 60%, at most 65%, at most 70%, at most 75%, at most 80%, at most 85%, At most 90% or at most 95%.This embodiment it is other in terms of other in, the effective quantity of disclosed plant agent composition is Be enough to infect plant group in the murderous amount of following virulence factor：For example, about 10% to about 20%, about 10% To about 30%, about 10% to about 40%, about 10% to about 50%, about 10% to about 60%, about 10% to about 70%, about 10% to About 80%, about 10% to about 90%, about 10% to about 95%, about 20% to about 30%, about 20% to about 40%, about 20% to about 50%th, about 20% to about 60%, about 20% to about 70%, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%th, about 30% to about 40%, about 30% to about 50%, about 30% to about 60%, about 30% to about 70%, about 30% to about 80%th, about 30% to about 90%, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%, about 40% to about 70%th, about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%, about 50% to about 70%th, about 50% to about 80%, about 50% to about 90%, about 50% to about 95%, about 60% to about 70%, about 60% to about 80%th, about 60% to about 90%, about 60% to about 95%, about 70% to about 80%, about 70% to about 90%, about 70% to about 95%th, about 80% to about 90%, about 80% to about 95% or about 90% to about 95%.
The effective quantity of disclosed plant agent composition can be enough to reduce the group for seeking controlled virulence factor The amount of size.In in terms of this embodiment, the effective quantity of disclosed plant agent composition is to be enough to reduce to seek to be controlled Virulence factor group size following amount：For example, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%th, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90% or about 95%.In in terms of other of this embodiment, the effective quantity of disclosed plant agent composition is to be enough to reduce to seek Seek the following amount of the size of the group of controlled virulence factor：For example, at least 10%, at least 15%, at least 20%, at least 25%th, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, At least 70%, at least 75%, at least 80%, at least 85%, at least 90% or at least 95%.In its other party again of this embodiment In face, the effective quantity of disclosed plant agent composition be enough to reduce the size for the group for seeking controlled virulence factor with Under amount：Such as at most 10%, at most 15%, at most 20%, at most 25%, at most 30%, at most 35%, at most 40%, extremely More 45%, at most 50%, at most 55%, at most 60%, at most 65%, at most 70%, at most 75%, at most 80%, at most 85%th, at most 90% or at most 95%.This embodiment it is other in terms of other in, disclosed plant agent composition it is effective Amount is the following amount for being enough to reduce the size for the group for seeking controlled virulence factor：For example, about 10% to about 20%, about 10% to about 30%, about 10% to about 40%, about 10% to about 50%, about 10% to about 60%, about 10% to about 70%, about 10% to about 80%, about 10% to about 90%, about 10% to about 95%, about 20% to about 30%, about 20% to about 40%, about 20% to about 50%, about 20% to about 60%, about 20% to about 70%, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%, about 30% to about 40%, about 30% to about 50%, about 30% to about 60%, about 30% to about 70%, about 30% to about 80%, about 30% to about 90%, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%, about 40% to about 70%, about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%, about 50% to about 70%, about 50% to about 80%, about 50% to about 90%, about 50% to about 95%, about 60% to about 70%, about 60% to about 80%, about 60% to about 90%, about 60% to about 95%, about 70% to about 80%, about 70% to about 90%, about 70% to about 95%, about 80% to about 90%, about 80% to about 95% or about 90% to about 95%.
The effective quantity of disclosed plant agent composition can be the group that is enough to prevent to seek controlled virulence factor into Enter or infect the amount in one or more places.In in terms of this embodiment, the effective quantity of disclosed plant agent composition It is the amount for being enough the group of following virulence factor is prevented to enter or infect one or more places：For example, about 10%, about 15%th, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%th, about 75%, about 80%, about 85%, about 90% or about 95%.In in terms of other of this embodiment, disclosed plant The effective quantity of agent composition is the amount for being enough the group of following virulence factor is prevented to enter or infect one or more places： For example, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, At least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90% or at least 95%.This embodiment again in terms of other in, the effective quantity of disclosed plant agent composition is to be enough to hinder Only the group of following virulence factor enters or infects the amount in one or more places：Such as at most 10%, at most 15%, extremely More 20%, at most 25%, at most 30%, at most 35%, at most 40%, at most 45%, at most 50%, at most 55%, at most 60%th, at most 65%, at most 70%, at most 75%, at most 80%, at most 85%, at most 90% or at most 95%.Implement herein In other other aspects of scheme, the effective quantity of disclosed plant agent composition is the group for being enough to prevent following virulence factor Into or infect the amount in one or more places：For example, about 10% to about 20%, about 10% to about 30%, about 10% to about 40%th, about 10% to about 50%, about 10% to about 60%, about 10% to about 70%, about 10% to about 80%, about 10% to about 90%th, about 10% to about 95%, about 20% to about 30%, about 20% to about 40%, about 20% to about 50%, about 20% to about 60%th, about 20% to about 70%, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%, about 30% to about 40%th, about 30% to about 50%, about 30% to about 60%, about 30% to about 70%, about 30% to about 80%, about 30% to about 90%th, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%, about 40% to about 70%, about 40% to about 80%th, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%, about 50% to about 70%, about 50% to about 80%th, about 50% to about 90%, about 50% to about 95%, about 60% to about 70%, about 60% to about 80%, about 60% to about 90%th, about 60% to about 95%, about 70% to about 80%, about 70% to about 90%, about 70% to about 95%, about 80% to about 90%th, about 80% to about 95% or about 90% to about 95%.
The effective quantity of disclosed plant agent composition can be enough to improve by the water of root hair, minerals and from soil The amount of the absorption of other nutrients of earth.In in terms of this embodiment, the effective quantity of disclosed plant agent composition is foot To improve the following amount of the absorption by the water of root hair, minerals and other nutrients from soil：For example, about 10%, About 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, About 70%, about 75%, about 80%, about 85%, about 90% or about 95%.In in terms of other of this embodiment, disclosed plant The effective quantity of agent composition is the absorption for being enough to improve through the water of root hair, minerals and other nutrients from soil Following amount：For example, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%th, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, At least 85%, at least 90% or at least 95%.This embodiment it is other in terms of other in, disclosed plant agent composition Effective quantity is the following amount for being enough to improve the absorption by the water of root hair, minerals and other nutrients from soil： Such as at most 10%, at most 15%, at most 20%, at most 25%, at most 30%, at most 35%, at most 40%, at most 45%, At most 50%, at most 55%, at most 60%, at most 65%, at most 70%, at most 75%, at most 80%, at most 85%, at most 90% or at most 95%.This embodiment again in terms of other in, the effective quantity of disclosed plant agent composition is to be enough to change Into the following amount of the absorption by the water of root hair, minerals and other nutrients from soil：For example, about 10% to about 20%th, about 10% to about 30%, about 10% to about 40%, about 10% to about 50%, about 10% to about 60%, about 10% to about 70%th, about 10% to about 80%, about 10% to about 90%, about 10% to about 95%, about 20% to about 30%, about 20% to about 40%th, about 20% to about 50%, about 20% to about 60%, about 20% to about 70%, about 20% to about 80%, about 20% to about 90%th, about 20% to about 95%, about 30% to about 40%, about 30% to about 50%, about 30% to about 60%, about 30% to about 70%th, about 30% to about 80%, about 30% to about 90%, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%th, about 40% to about 70%, about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%th, about 50% to about 70%, about 50% to about 80%, about 50% to about 90%, about 50% to about 95%, about 60% to about 70%th, about 60% to about 80%, about 60% to about 90%, about 60% to about 95%, about 70% to about 80%, about 70% to about 90%th, about 70% to about 95%, about 80% to about 90%, about 80% to about 95% or about 90% to about 95%.
The effective quantity of disclosed plant agent composition can be enough to improve to flow by the xylem sap of xylem Amount.In in terms of this embodiment, the effective quantity of disclosed plant agent composition is to be enough to improve through the wooden of xylem The following amount of portion's juice flowing：For example, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%th, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90% or about 95%. In in terms of other of this embodiment, the effective quantity of disclosed plant agent composition is to be enough to improve through the wooden of xylem The following amount of portion's juice flowing：For example, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%th, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, At least 80%, at least 85%, at least 90% or at least 95%.This embodiment it is other in terms of other in, disclosed plant The effective quantity of agent composition is to be enough to improve the following amount flowed by the xylem sap of xylem：Such as at most 10%, At most 15%, at most 20%, at most 25%, at most 30%, at most 35%, at most 40%, at most 45%, at most 50%, at most 55%th, at most 60%, at most 65%, at most 70%, at most 75%, at most 80%, at most 85%, at most 90% or at most 95%.This embodiment again in terms of other in, the effective quantity of disclosed plant agent composition is to be enough to improve by wooden The following amount of the xylem sap flowing in portion：For example, about 10% to about 20%, about 10% to about 30%, about 10% to about 40%th, about 10% to about 50%, about 10% to about 60%, about 10% to about 70%, about 10% to about 80%, about 10% to about 90%th, about 10% to about 95%, about 20% to about 30%, about 20% to about 40%, about 20% to about 50%, about 20% to about 60%th, about 20% to about 70%, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%, about 30% to about 40%th, about 30% to about 50%, about 30% to about 60%, about 30% to about 70%, about 30% to about 80%, about 30% to about 90%th, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%, about 40% to about 70%, about 40% to about 80%th, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%, about 50% to about 70%, about 50% to about 80%th, about 50% to about 90%, about 50% to about 95%, about 60% to about 70%, about 60% to about 80%, about 60% to about 90%th, about 60% to about 95%, about 70% to about 80%, about 70% to about 90%, about 70% to about 95%, about 80% to about 90%th, about 80% to about 95% or about 90% to about 95%.
The effective quantity of disclosed plant agent composition can be enough to improve the amount that photosynthate flows in bast. In in terms of this embodiment, the effective quantity of disclosed plant agent composition is to be enough to improve photosynthate in bast The following amount of flowing：For example, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%th, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90% or about 95%.Implement herein In other aspects of scheme, the effective quantity of disclosed plant agent composition is to be enough to improve photosynthate in bast to flow Following amount：For example, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%th, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, At least 85%, at least 90% or at least 95%.This embodiment it is other in terms of other in, disclosed plant agent composition Effective quantity is to be enough to improve the following amount that photosynthate flows in bast：Such as at most 10%, at most 15%, at most 20%th, at most 25%, at most 30%, at most 35%, at most 40%, at most 45%, at most 50%, at most 55%, at most 60%, At most 65%, at most 70%, at most 75%, at most 80%, at most 85%, at most 90% or at most 95%.In this embodiment Other aspects again in, the effective quantity of disclosed plant agent composition is enough to improve photosynthate in bast and flows Following amount：For example, about 10% to about 20%, about 10% to about 30%, about 10% to about 40%, about 10% to about 50%, about 10% to about 60%, about 10% to about 70%, about 10% to about 80%, about 10% to about 90%, about 10% to about 95%, about 20% to about 30%, about 20% to about 40%, about 20% to about 50%, about 20% to about 60%, about 20% to about 70%, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%, about 30% to about 40%, about 30% to about 50%, about 30% to about 60%, about 30% to about 70%, about 30% to about 80%, about 30% to about 90%, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%, about 40% to about 70%, about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%, about 50% to about 70%, about 50% to about 80%, about 50% to about 90%, about 50% to about 95%, about 60% to about 70%, about 60% to about 80%, about 60% to about 90%, about 60% to about 95%, about 70% to about 80%, about 70% to about 90%, about 70% to about 95%, about 80% to about 90%, about 80% to about 95% or about 90% to about 95%.
The effective quantity of disclosed plant agent composition can be enough to increase water, minerals and other battalion from soil Support the amount of the absorption of object.In in terms of this embodiment, the effective quantity of disclosed plant agent composition is to be enough to increase water, ore deposit The following amount of the absorption of substance and other nutrients from soil：For example, about 10%, about 15%, about 20%, about 25%, About 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, About 85%, about 90% or about 95%.In in terms of other of this embodiment, the effective quantity of disclosed plant agent composition is foot To increase the following amount of the absorption of water, minerals and other nutrients from soil：For example, at least 10%, at least 15%th, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, At least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90% or at least 95%.Herein In other other aspects of embodiment, the effective quantity of disclosed plant agent composition is to be enough to increase water, minerals and come From the following amount of the absorption of other nutrients of soil：Such as at most 10%, at most 15%, at most 20%, at most 25%, extremely More 30%, at most 35%, at most 40%, at most 45%, at most 50%, at most 55%, at most 60%, at most 65%, at most 70%th, at most 75%, at most 80%, at most 85%, at most 90% or at most 95%.In this embodiment again in terms of other In, the effective quantity of disclosed plant agent composition is the suction for being enough to increase water, minerals and other nutrients from soil The following amount received：For example, about 10% to about 20%, about 10% to about 30%, about 10% to about 40%, about 10% to about 50%, About 10% to about 60%, about 10% to about 70%, about 10% to about 80%, about 10% to about 90%, about 10% to about 95%, about 20% to about 30%, about 20% to about 40%, about 20% to about 50%, about 20% to about 60%, about 20% to about 70%, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%, about 30% to about 40%, about 30% to about 50%, about 30% to about 60%, about 30% to about 70%, about 30% to about 80%, about 30% to about 90%, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%, about 40% to about 70%, about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%, about 50% to about 70%, about 50% to about 80%, about 50% to about 90%, about 50% to about 95%, about 60% to about 70%, about 60% to about 80%, about 60% to about 90%, about 60% to about 95%, about 70% to about 80%, about 70% to about 90%, about 70% to about 95%, about 80% to about 90%, about 80% to about 95% or about 90% to about 95%.
The effective quantity of disclosed plant agent composition can be the capillarity and/or hydrostatic for being enough to increase in xylem The amount of pressure.In in terms of this embodiment, the effective quantity of disclosed plant agent composition is the hair for being enough to increase in xylem The following amount of capillary action and/or hydrostatic pressing：For example, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, About 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90% Or about 95%.In in terms of other of this embodiment, the effective quantity of disclosed plant agent composition is to be enough to increase xylem In capillarity and/or hydrostatic pressing following amount：For example, at least 10%, at least 15%, at least 20%, at least 25%, At least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%th, at least 75%, at least 80%, at least 85%, at least 90% or at least 95%.In the other in terms of other of this embodiment In, the effective quantity of disclosed plant agent composition is be enough to increase capillarity and/or hydrostatic pressing in xylem following Amount：Such as at most 10%, at most 15%, at most 20%, at most 25%, at most 30%, at most 35%, at most 40%, at most 45%th, at most 50%, at most 55%, at most 60%, at most 65%, at most 70%, at most 75%, at most 80%, at most 85%, At most 90% or at most 95%.This embodiment again in terms of other in, the effective quantity of disclosed plant agent composition is foot To increase the following amount of capillarity and/or hydrostatic pressing in xylem：For example, about 10% to about 20%, about 10% to about 30%th, about 10% to about 40%, about 10% to about 50%, about 10% to about 60%, about 10% to about 70%, about 10% to about 80%th, about 10% to about 90%, about 10% to about 95%, about 20% to about 30%, about 20% to about 40%, about 20% to about 50%th, about 20% to about 60%, about 20% to about 70%, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%th, about 30% to about 40%, about 30% to about 50%, about 30% to about 60%, about 30% to about 70%, about 30% to about 80%th, about 30% to about 90%, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%, about 40% to about 70%th, about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%, about 50% to about 70%th, about 50% to about 80%, about 50% to about 90%, about 50% to about 95%, about 60% to about 70%, about 60% to about 80%th, about 60% to about 90%, about 60% to about 95%, about 70% to about 80%, about 70% to about 90%, about 70% to about 95%th, about 80% to about 90%, about 80% to about 95% or about 90% to about 95%.
The effective quantity of disclosed plant agent composition can be the amount for being enough to improve the transport that raw material pass through plant.Herein In the aspect of embodiment, the effective quantity of disclosed plant agent composition be enough to improve the transport that raw material pass through plant with Under amount：For example, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%th, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90% or about 95%.In this embodiment In other aspects, the effective quantity of disclosed plant agent composition is to be enough to improve the following of the transport that raw material pass through plant Amount：For example, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%th, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, At least 90% or at least 95%.This embodiment it is other in terms of other in, the effective quantity of disclosed plant agent composition is It is enough to improve the following amount for the transport that raw material pass through plant：Such as at most 10%, at most 15%, at most 20%, at most 25%th, at most 30%, at most 35%, at most 40%, at most 45%, at most 50%, at most 55%, at most 60%, at most 65%, At most 70%, at most 75%, at most 80%, at most 85%, at most 90% or at most 95%.In its other party again of this embodiment In face, the effective quantity of disclosed plant agent composition is to be enough to improve the following amount for the transport that raw material pass through plant：Such as About 10% to about 20%, about 10% to about 30%, about 10% to about 40%, about 10% to about 50%, about 10% to about 60%, about 10% to about 70%, about 10% to about 80%, about 10% to about 90%, about 10% to about 95%, about 20% to about 30%, about 20% to about 40%, about 20% to about 50%, about 20% to about 60%, about 20% to about 70%, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%, about 30% to about 40%, about 30% to about 50%, about 30% to about 60%, about 30% to about 70%, about 30% to about 80%, about 30% to about 90%, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%, about 40% to about 70%, about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%, about 50% to about 70%, about 50% to about 80%, about 50% to about 90%, about 50% to about 95%, about 60% to about 70%, about 60% to about 80%, about 60% to about 90%, about 60% to about 95%, about 70% to about 80%, about 70% to about 90%, about 70% to about 95%, about 80% to about 90%, about 80% to about 95% or about 90% to about 95%.
The effective quantity of disclosed plant agent composition can be the amount for being enough to increase the synthesis of compound and energy in plant. In in terms of this embodiment, the effective quantity of disclosed plant agent composition is enough to increase compound and energy in plant The following amount of synthesis：For example, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%th, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90% or about 95%.Implement herein In other aspects of scheme, the effective quantity of disclosed plant agent composition is the synthesis for being enough to increase compound and energy in plant Following amount：For example, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%th, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, At least 85%, at least 90% or at least 95%.This embodiment it is other in terms of other in, disclosed plant agent composition Effective quantity is the following amount for being enough to increase the synthesis of compound and energy in plant：Such as at most 10%, at most 15%, at most 20%th, at most 25%, at most 30%, at most 35%, at most 40%, at most 45%, at most 50%, at most 55%, at most 60%, At most 65%, at most 70%, at most 75%, at most 80%, at most 85%, at most 90% or at most 95%.In this embodiment Again other aspect in, the effective quantity of disclosed plant agent composition is the synthesis for being enough to increase compound and energy in plant Following amount：For example, about 10% to about 20%, about 10% to about 30%, about 10% to about 40%, about 10% to about 50%, about 10% to about 60%, about 10% to about 70%, about 10% to about 80%, about 10% to about 90%, about 10% to about 95%, about 20% to about 30%, about 20% to about 40%, about 20% to about 50%, about 20% to about 60%, about 20% to about 70%, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%, about 30% to about 40%, about 30% to about 50%, about 30% to about 60%, about 30% to about 70%, about 30% to about 80%, about 30% to about 90%, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%, about 40% to about 70%, about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%, about 50% to about 70%, about 50% to about 80%, about 50% to about 90%, about 50% to about 95%, about 60% to about 70%, about 60% to about 80%, about 60% to about 90%, about 60% to about 95%, about 70% to about 80%, about 70% to about 90%, about 70% to about 95%, about 80% to about 90%, about 80% to about 95% or about 90% to about 95%.
The effective quantity of disclosed plant agent composition can be enough to improve maintenance and continue the chemical combination needed for plant growth The amount of the synthesis of object and energy.In in terms of this embodiment, the effective quantity of disclosed plant agent composition is to be enough to improve The following amount of the synthesis of compound and energy needed for maintenance and continuation plant growth：For example, about 10%, about 15%, about 20%th, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%th, about 80%, about 85%, about 90% or about 95%.In in terms of other of this embodiment, disclosed plant agent composition Effective quantity be the synthesis for being enough to improve maintenance and continuing compound and energy needed for plant growth following amount：Such as extremely Few 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%th, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90% Or at least 95%.This embodiment it is other in terms of other in, the effective quantity of disclosed plant agent composition is to be enough to improve The following amount of the synthesis of compound and energy needed for maintenance and continuation plant growth：Such as at most 10%, at most 15%, extremely More 20%, at most 25%, at most 30%, at most 35%, at most 40%, at most 45%, at most 50%, at most 55%, at most 60%th, at most 65%, at most 70%, at most 75%, at most 80%, at most 85%, at most 90% or at most 95%.Implement herein In other aspects again of scheme, the effective quantity of disclosed plant agent composition is to be enough to improve maintenance and continue needed for plant growth Compound and energy synthesis following amount：For example, about 10% to about 20%, about 10% to about 30%, about 10% to about 40%th, about 10% to about 50%, about 10% to about 60%, about 10% to about 70%, about 10% to about 80%, about 10% to about 90%th, about 10% to about 95%, about 20% to about 30%, about 20% to about 40%, about 20% to about 50%, about 20% to about 60%th, about 20% to about 70%, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%, about 30% to about 40%th, about 30% to about 50%, about 30% to about 60%, about 30% to about 70%, about 30% to about 80%, about 30% to about 90%th, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%, about 40% to about 70%, about 40% to about 80%th, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%, about 50% to about 70%, about 50% to about 80%th, about 50% to about 90%, about 50% to about 95%, about 60% to about 70%, about 60% to about 80%, about 60% to about 90%th, about 60% to about 95%, about 70% to about 80%, about 70% to about 90%, about 70% to about 95%, about 80% to about 90%th, about 80% to about 95% or about 90% to about 95%.
The effective quantity of disclosed plant agent composition can be enough is dissolving, dispersion or otherwise remove destroy it is wooden The amount for one or more of components that xylem sap flows in portion.In in terms of this embodiment, disclosed plant agent group The effective quantity for closing object be enough to dissolve, disperse or otherwise remove destroy xylem sap flows in xylem one kind or The following amount of more kinds of components：For example, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%th, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90% or about 95%. In in terms of other of this embodiment, the effective quantity of disclosed plant agent composition is to be enough to dissolve, disperse or with its other party Formula removes the following amount for destroying one or more of components that xylem sap flows in xylem：For example, at least 10%, extremely Few 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%th, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90% or at least 95%.This embodiment it is other in terms of other in, the effective quantity of disclosed plant agent composition be enough to dissolve, disperse or Otherwise remove the following amount for destroying one or more of components that xylem sap flows in xylem：Such as at most 10%th, at most 15%, at most 20%, at most 25%, at most 30%, at most 35%, at most 40%, at most 45%, at most 50%, At most 55%, at most 60%, at most 65%, at most 70%, at most 75%, at most 80%, at most 85%, at most 90% or at most 95%.This embodiment again in terms of other in, the effective quantity of disclosed plant agent composition be enough to dissolve, disperse or with Other modes remove the following amount for destroying one or more of components that xylem sap flows in xylem：For example, about 10% To about 20%, about 10% to about 30%, about 10% to about 40%, about 10% to about 50%, about 10% to about 60%, about 10% to About 70%, about 10% to about 80%, about 10% to about 90%, about 10% to about 95%, about 20% to about 30%, about 20% to about 40%th, about 20% to about 50%, about 20% to about 60%, about 20% to about 70%, about 20% to about 80%, about 20% to about 90%th, about 20% to about 95%, about 30% to about 40%, about 30% to about 50%, about 30% to about 60%, about 30% to about 70%th, about 30% to about 80%, about 30% to about 90%, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%th, about 40% to about 70%, about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%th, about 50% to about 70%, about 50% to about 80%, about 50% to about 90%, about 50% to about 95%, about 60% to about 70%th, about 60% to about 80%, about 60% to about 90%, about 60% to about 95%, about 70% to about 80%, about 70% to about 90%th, about 70% to about 95%, about 80% to about 90%, about 80% to about 95% or about 90% to about 95%.
The effective quantity of disclosed plant agent composition can be enough to dissolve, disperse or otherwise remove destruction bast The amount for one or more of components that photosynthate flows in portion.In in terms of this embodiment, disclosed plant agent The effective quantity of composition is to be enough to dissolve, disperse or otherwise remove to destroy one of photosynthate flowing in bast The following amount of kind or more kind component：For example, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%th, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90% or About 95%.In in terms of other of this embodiment, the effective quantity of disclosed plant agent composition be enough to dissolve, disperse or with Other modes remove the following amount for destroying one or more of components that photosynthate flows in bast：For example, at least 10%th, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, At least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90% or at least 95%.This embodiment it is other in terms of other in, the effective quantity of disclosed plant agent composition be enough to dissolve, disperse or Otherwise remove the following amount for destroying one or more of components that photosynthate flows in bast：Such as extremely More 10%, at most 15%, at most 20%, at most 25%, at most 30%, at most 35%, at most 40%, at most 45%, at most 50%th, at most 55%, at most 60%, at most 65%, at most 70%, at most 75%, at most 80%, at most 85%, at most 90% Or at most 95%.This embodiment again in terms of other in, the effective quantity of disclosed plant agent composition be enough to dissolve, point It dissipates or otherwise removes the following amount for destroying one or more of components that photosynthate flows in bast：Example Such as from about 10% to about 20%, about 10% to about 30%, about 10% to about 40%, about 10% to about 50%, about 10% to about 60%, About 10% to about 70%, about 10% to about 80%, about 10% to about 90%, about 10% to about 95%, about 20% to about 30%, about 20% to about 40%, about 20% to about 50%, about 20% to about 60%, about 20% to about 70%, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%, about 30% to about 40%, about 30% to about 50%, about 30% to about 60%, about 30% to about 70%, about 30% to about 80%, about 30% to about 90%, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%, about 40% to about 70%, about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%, about 50% to about 70%, about 50% to about 80%, about 50% to about 90%, about 50% to about 95%, about 60% to about 70%, about 60% to about 80%, about 60% to about 90%, about 60% to about 95%, about 70% to about 80%, about 70% to about 90%, about 70% to about 95%, about 80% to about 90%, about 80% to about 95% or about 90% to about 95%.
The effective quantity of disclosed plant agent composition can be enough to dissolve, disperse or otherwise remove destruction irrigation The amount of one or more of components of water flowing in the pipeline network of system.In in terms of this embodiment, disclosed plant The effective quantity of agent composition is to be enough to dissolve, disperse or otherwise remove water flowing in the pipeline network for destroying irrigation system One or more of components following amount：For example, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%th, about 45%, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90% or About 95%.In in terms of other of this embodiment, the effective quantity of disclosed plant agent composition be enough to dissolve, disperse or with Other modes remove the following amount of one or more of components of water flowing in the pipeline network for destroying irrigation system：Such as extremely Few 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%th, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90% Or at least 95%.This embodiment it is other in terms of other in, the effective quantity of disclosed plant agent composition be enough to dissolve, One or more of components of water flowing is following in dispersion or the otherwise pipeline network of removing destruction irrigation system Amount：Such as at most 10%, at most 15%, at most 20%, at most 25%, at most 30%, at most 35%, at most 40%, at most 45%th, at most 50%, at most 55%, at most 60%, at most 65%, at most 70%, at most 75%, at most 80%, at most 85%, At most 90% or at most 95%.This embodiment again in terms of other in, the effective quantity of disclosed plant agent composition is foot To dissolve, disperse or otherwise remove one or more of components of water flowing in the pipeline network for destroying irrigation system Following amount：For example, about 10% to about 20%, about 10% to about 30%, about 10% to about 40%, about 10% to about 50%, about 10% to about 60%, about 10% to about 70%, about 10% to about 80%, about 10% to about 90%, about 10% to about 95%, about 20% to about 30%, about 20% to about 40%, about 20% to about 50%, about 20% to about 60%, about 20% to about 70%, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%, about 30% to about 40%, about 30% to about 50%, about 30% to about 60%, about 30% to about 70%, about 30% to about 80%, about 30% to about 90%, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%, about 40% to about 70%, about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%, about 50% to about 70%, about 50% to about 80%, about 50% to about 90%, about 50% to about 95%, about 60% to about 70%, about 60% to about 80%, about 60% to about 90%, about 60% to about 95%, about 70% to about 80%, about 70% to about 90%, about 70% to about 95%, about 80% to about 90%, about 80% to about 95% or about 90% to about 95%.
The effective quantity of disclosed plant agent composition can be the amount for being enough to improve water transport in entire pipeline network.Herein In the aspect of embodiment, the effective quantity of disclosed plant agent composition be enough to improve water transport in entire pipeline network with Under amount：For example, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 55%th, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90% or about 95%.In this embodiment In other aspects, the effective quantity of disclosed plant agent composition is be enough to improve water transport in entire pipeline network following Amount：For example, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%th, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, At least 90% or at least 95%.This embodiment it is other in terms of other in, the effective quantity of disclosed plant agent composition is It is enough to improve the following amount of water transport in entire pipeline network：Such as at most 10%, at most 15%, at most 20%, at most 25%th, at most 30%, at most 35%, at most 40%, at most 45%, at most 50%, at most 55%, at most 60%, at most 65%, At most 70%, at most 75%, at most 80%, at most 85%, at most 90% or at most 95%.In its other party again of this embodiment In face, the effective quantity of disclosed plant agent composition is the following amount for being enough to improve water transport in entire pipeline network：Such as About 10% to about 20%, about 10% to about 30%, about 10% to about 40%, about 10% to about 50%, about 10% to about 60%, about 10% to about 70%, about 10% to about 80%, about 10% to about 90%, about 10% to about 95%, about 20% to about 30%, about 20% to about 40%, about 20% to about 50%, about 20% to about 60%, about 20% to about 70%, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%, about 30% to about 40%, about 30% to about 50%, about 30% to about 60%, about 30% to about 70%, about 30% to about 80%, about 30% to about 90%, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%, about 40% to about 70%, about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%, about 50% to about 70%, about 50% to about 80%, about 50% to about 90%, about 50% to about 95%, about 60% to about 70%, about 60% to about 80%, about 60% to about 90%, about 60% to about 95%, about 70% to about 80%, about 70% to about 90%, about 70% to about 95%, about 80% to about 90%, about 80% to about 95% or about 90% to about 95%.
The effective quantity of disclosed plant agent composition can be the health and vigor that is enough to keep and/or enhance plant Amount.In in terms of this embodiment, the effective quantity of disclosed plant agent composition is be enough to keep and/or enhance plant strong The following amount of health and vigor：For example, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%th, about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90% or about 95%. In in terms of other of this embodiment, the effective quantity of disclosed plant agent composition is enough to keep and/or enhance plant The following amount of health and vigor：For example, at least 10%, at least 15%, at least 20%, at least 25%, at least 30%, at least 35%th, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, At least 80%, at least 85%, at least 90% or at least 95%.This embodiment it is other in terms of other in, disclosed plant The effective quantity of agent composition is the health that is enough to keep and/or enhance plant and the following amount of vigor：Such as at most 10%, extremely More 15%, at most 20%, at most 25%, at most 30%, at most 35%, at most 40%, at most 45%, at most 50%, at most 55%th, at most 60%, at most 65%, at most 70%, at most 75%, at most 80%, at most 85%, at most 90% or at most 95%.This embodiment again in terms of other in, the effective quantity of disclosed plant agent composition is to be enough to keep and/or enhance The health of plant and the following amount of vigor：For example, about 10% to about 20%, about 10% to about 30%, about 10% to about 40%, About 10% to about 50%, about 10% to about 60%, about 10% to about 70%, about 10% to about 80%, about 10% to about 90%, about 10% to about 95%, about 20% to about 30%, about 20% to about 40%, about 20% to about 50%, about 20% to about 60%, about 20% to about 70%, about 20% to about 80%, about 20% to about 90%, about 20% to about 95%, about 30% to about 40%, about 30% to about 50%, about 30% to about 60%, about 30% to about 70%, about 30% to about 80%, about 30% to about 90%, about 30% to about 95%, about 40% to about 50%, about 40% to about 60%, about 40% to about 70%, about 40% to about 80%, about 40% to about 90%, about 40% to about 95%, about 50% to about 60%, about 50% to about 70%, about 50% to about 80%, about 50% to about 90%, about 50% to about 95%, about 60% to about 70%, about 60% to about 80%, about 60% to about 90%, about 60% to about 95%, about 70% to about 80%, about 70% to about 90%, about 70% to about 95%, about 80% to about 90%, about 80% to about 95% or about 90% to about 95%.
The effective quantity of disclosed plant agent composition can be the dilution of plant agent composition disclosed herein.It is real herein In the aspect for applying scheme, the effective quantity of disclosed plant agent composition is following plant agent composition:Diluent ratio：Such as About 1:50th, about 1:75th, about 1:100th, about 1:125th, about 1:150th, about 1:175th, about 1:200th, about 1:225th, about 1:250th, about 1: 275th, about 1:300th, about 1:325th, about 1:350th, about 1:375th, about 1:400th, about 1:425th, about 1:450th, about 1:475th, about 1:500、 About 1:525th, about 1:550th, about 1:575 or about 1:600.In in terms of other of this embodiment, disclosed plant agent composition Effective quantity be following plant agent composition:Diluent ratio：For example, at least 1:50th, at least 1:75th, at least 1:100th, at least 1:125th, at least 1:150th, at least 1:175th, at least 1:200th, at least 1:225th, at least 1:250th, at least 1:275th, at least 1:300、 At least 1:325th, at least 1:350th, at least 1:375th, at least 1:400th, at least 1:425th, at least 1:450th, at least 1:475th, at least 1: 500th, at least 1:525th, at least 1:550th, at least 1:575 or at least 1:600.It is disclosed in terms of other of this embodiment The effective quantity of plant agent composition is following plant agent composition:Diluent ratio：Such as at most 1:50th, at most 1:75th, extremely More 1:100th, at most 1:125th, at most 1:150th, at most 1:175th, at most 1:200th, at most 1:225th, at most 1:250th, at most 1: 275th, at most 1:300th, at most 1:325th, at most 1:350th, at most 1:375th, at most 1:400th, at most 1:425th, at most 1:450th, extremely More 1:475th, at most 1:500th, at most 1:525th, at most 1:550th, at most 1:575 or at most 1:600.In other of this embodiment In aspect, the effective quantity of disclosed plant agent composition is following plant agent composition:Diluent ratio：For example, about 1:50 to About 1:100th, about 1:50 to about 1:200th, about 1:50 to about 1:300th, about 1:50 to about 1:400th, about 1:50 to about 1:500th, about 1: 50 to about 1:600th, about 1:100 to about 1:200th, about 1:100 to about 1:300th, about 1:100 to about 1:400th, about 1:100 to about 1: 500th, about 1:100 to about 1:600th, about 1:200 to about 1:300th, about 1:200 to about 1:400th, about 1:200 to about 1:500th, about 1: 200 to about 1:600th, about 1:300 to about 1:400th, about 1:300 to about 1:500th, about 1:300 to about 1:600th, about 1:400 to about 1: 500th, about 1:400 to about 1:600 or about 1:500 to about 1:600.These plant agent compositions:Diluent ratio is typically dense Degree, the concentration are the method for the virulence factor of disclosed control plant disease, purposes and are planted for disclosed increase Object is grown and/or the method and purposes of crop production are with the purposes of the virulence factor of control plant disease and for disclosed guarantor The method for the efficiency held or improved the irrigation system and the effective quantity of purposes.
In in terms of this embodiment, the effective quantity of disclosed plant agent composition is following plant agent composition: Diluent ratio：For example, about 1:500th, about 1:750th, about 1:1000th, about 1:1250th, about 1:1500th, about 1:1750th, about 1:2000、 About 1:2250th, about 1:2500th, about 1:2750th, about 1:3000th, about 1:3250th, about 1:3500th, about 1:3750th, about 1:4000th, about 1: 4250th, about 1:4500th, about 1:4750th, about 1:5000th, about 1:5250th, about 1:5500th, about 1:5750th, about 1:6000th, about 1:7000、 About 1:8000th, about 1:9000 or about 1:10000.In in terms of other of this embodiment, disclosed plant agent composition has Effect amount is following plant agent composition:Diluent ratio：For example, at least 1:500th, at least 1:750th, at least 1:1000th, at least 1: 1250th, at least 1:1500th, at least 1:1750th, at least 1:2000th, at least 1:2250th, at least 1:2500th, at least 1:2750th, at least 1: 3000th, at least 1:3250th, at least 1:3500th, at least 1:3750th, at least 1:4000th, at least 1:4250th, at least 1:4500th, at least 1: 4750th, at least 1:5000th, at least 1:5250th, at least 1:5500th, at least 1:5750th, at least 1:6000th, at least 1:7000th, at least 1: 8000th, at least 1:9000 or at least 1:10000.This embodiment again in terms of other in, the effective quantity of disclosed composition It is following plant agent composition:Diluent ratio：Such as at most 1:500th, at most 1:750th, at most 1:1000th, at most 1: 1250th, at most 1:1500th, at most 1:1750th, at most 1:2000th, at most 1:2250th, at most 1:2500th, at most 1:2750th, at most 1: 3000th, at most 1:3250th, at most 1:3500th, at most 1:3750th, at most 1:4000th, at most 1:4250th, at most 1:4500th, at most 1: 4750th, at most 1:5000th, at most 1:5250th, at most 1:5500th, at most 1:5750th, at most 1:6000th, at most 1:7000th, at most 1: 8000th, at most 1:9000 or at most 1:10000.In terms of other of this embodiment, disclosed plant agent composition it is effective Amount is following plant agent composition:Diluent ratio：For example, about 1:500 to about 1:1000th, about 1:500 to about 1:2000th, about 1:500 to about 1:3000th, about 1:500 to about 1:4000th, about 1:500 to about 1:5000th, about 1:500 to about 1:6000th, about 1:500 To about 1:7000th, about 1:500 to about 1:8000th, about 1:500 to about 1:9000th, about 1:500 to about 1:10000th, about 1:1000 to About 1:2000th, about 1:1000 to about 1:3000th, about 1:1000 to about 1:4000th, about 1:1000 to about 1:5000th, about 1:1000 to About 1:6000th, about 1:1000 to about 1:7000th, about 1:1000 to about 1:8000th, about 1:1000 to about 1:9000th, about 1:1000 to About 1:10000th, about 1:2000 to about 1:3000th, about 1:2000 to about 1:4000th, about 1:2000 to about 1:5000th, about 1:2000 to About 1:6000th, about 1:2000 to about 1:7000th, about 1:2000 to about 1:8000th, about 1:2000 to about 1:9000th, about 1:2000 to About 1:10000th, about 1:3000 to about 1:4000th, about 1:3000 to about 1:5000th, about 1:3000 to about 1:6000th, about 1:3000 to About 1:7000th, about 1:3000 to about 1:8000th, about 1:3000 to about 1:9000th, about 1:3000 to about 1:10000th, about 1:4000 to About 1:5000th, about 1:4000 to about 1:6000th, about 1:4000 to about 1:7000th, about 1:4000 to about 1:8000th, about 1:4000 to About 1:9000th, about 1:4000 to about 1:10000th, about 1:5000 to about 1:6000th, about 1:5000 to about 1:7000th, about 1:5000 to About 1:8000th, about 1:5000 to about 1:9000th, about 1:5000 to about 1:10000th, about 1:6000 to about 1:7000th, about 1:6000 to About 1:8000th, about 1:6000 to about 1:9000th, about 1:6000 to about 1:10000th, about 1:7000 to about 1:8000th, about 1:7000 to About 1:9000th, about 1:7000 to about 1:10000th, about 1:8000 to about 1:9000th, about 1:8000 to about 1:10000 or about 1:9000 To about 1:10000.These plant agent compositions:Diluent ratio is typically concentration, and the concentration is for disclosed control The method of the virulence factor of plant disease, purposes and for disclosed increase plant growth and/or crop production method and Purposes is with the purposes of the virulence factor of control plant disease and for disclosed holding or the side of efficiency to improve the irrigation system The effective quantity of method and purposes.
In in terms of this embodiment, the effective quantity of disclosed plant agent composition has following ultimate density：Example Such as from about 0.0001%, about 0.0002%, about 0.0003%, about 0.0004%, about 0.0005%, about 0.0006%, about 0.0007%th, about 0.0008%, about 0.0009%, about 0.001%, about 0.002%, about 0.003%, about 0.004%, about 0.005%th, about 0.006%, about 0.007%, about 0.008%, about 0.009%, about 0.01%, about 0.02%, about 0.03%, about 0.04%th, about 0.05%, about 0.06%, about 0.07%, about 0.08%, about 0.09%, about 0.1%, about 0.2%, about 0.3%, About 0.4%, about 0.5%, about 0.6%, about 0.7%, about 0.8%, about 0.9%, about 1%, about 2%, about 3%, about 4%, about 5%th, about 6%, about 7%, about 8%, about 9% or about 10%.In in terms of other of this embodiment, disclosed plant agent combination The effective quantity of object has following ultimate density：For example, at least 0.0001%, at least 0.0002%, at least 0.0003%, at least 0.0004%th, at least 0.0005%, at least 0.0006%, at least 0.0007%, at least 0.0008%, at least 0.0009%, extremely Few 0.001%, at least 0.002%, at least 0.003%, at least 0.004%, at least 0.005%, at least 0.006%, at least 0.007%th, at least 0.008%, at least 0.009%, at least 0.01%, at least 0.02%, at least 0.03%, at least 0.04%, At least 0.05%, at least 0.06%, at least 0.07%, at least 0.08%, at least 0.09%, at least 0.1%, at least 0.2%, extremely Few 0.3%, at least 0.4%, at least 0.5%, at least 0.6%, at least 0.7%, at least 0.8%, at least 0.9%, at least 1%, At least 2%, at least 3%, at least 4%, at least 5%, at least 6%, at least 7%, at least 8%, at least 9% or at least 10%. In other aspects again of this embodiment, the effective quantity of disclosed plant agent composition has following ultimate density：Such as extremely More 0.0001%, at most 0.0002%, at most 0.0003%, at most 0.0004%, at most 0.0005%, at most 0.0006%, At most 0.0007%, at most 0.0008%, at most 0.0009%, at most 0.001%, at most 0.002%, at most 0.003%, extremely More 0.004%, at most 0.005%, at most 0.006%, at most 0.007%, at most 0.008%, at most 0.009%, at most 0.01%th, at most 0.02%, at most 0.03%, at most 0.04%, at most 0.05%, at most 0.06%, at most 0.07%, at most 0.08%th, at most 0.09%, at most 0.1%, at most 0.2%, at most 0.3%, at most 0.4%, at most 0.5%, at most 0.6%th, at most 0.7%, at most 0.8%, at most 0.9%, at most 1%, at most 2%, at most 3%, at most 4%, at most 5%, At most 6%, at most 7%, at most 8%, at most 9% or at most 10%.This embodiment it is other in terms of other in, it is disclosed The effective quantity of plant agent composition has following ultimate density：For example, about 0.0001% to about 0.0005%, about 0.0001% To about 0.001%, about 0.0001% to about 0.005%, about 0.0001% to about 0.01%, about 0.0001% to about 0.05%, About 0.0001% to about 0.1%, about 0.0001% to about 0.5%, about 0.0001% to about 1%, about 0.0001% to about 5%, About 0.0001% to about 10%, about 0.0005% to about 0.001%, about 0.0005% to about 0.005%, about 0.0005% to about 0.01%th, about 0.0005% to about 0.05%, about 0.0005% to about 0.1%, about 0.0005% to about 0.5%, about 0.0005% to about 1%, about 0.0005% to about 5%, about 0.0005% to about 10%, about 0.001% to about 0.005%, about 0.001% to about 0.01%, about 0.001% to about 0.05%, about 0.001% to about 0.1%, about 0.001% to about 0.5%, About 0.001% to about 1%, about 0.001% to about 5%, about 0.001% to about 10%, about 0.005% to about 0.01%, about 0.005% to about 0.05%, about 0.005% to about 0.1%, about 0.005% to about 0.5%, about 0.005% to about 1%, about 0.005% to about 5%, about 0.005% to about 10%, about 0.01% to about 0.05%, about 0.01% to about 0.1%, about 0.01% to about 0.5%, about 0.01% to about 1%, about 0.01% to about 5%, about 0.01% to about 10%, about 0.05% to about 0.1%th, about 0.05% to about 0.5%, about 0.05% to about 1%, about 0.05% to about 5%, about 0.05% to about 10%, about 0.1% to about 0.5%, about 0.1% to about 1%, about 0.1% to about 5%, about 0.1% to about 10%, about 0.5% to about 1%, About 0.5% to about 5%, about 0.5% to about 10%, about 1% to about 5%, about 1% to about 10% or about 5% to about 10%.
The effect of plant agent composition disclosed herein can by determine adverse effect, the death rate, reduction it is pathogenic because Sub-group, one or more places reduced entrance or infect or any other lesion assessment to virulence factor group It monitors, described other lesion assessments include but not limited to inhibition, prevention or the sluggishness of virulence factor growth, virulence factor regeneration Sluggishness or virulence factor development inhibition, prevention or sluggishness, it is all these all to be covered by term " control ".Effect is also logical It crosses the phytotoxicity of the plant to being infected by virulence factor group, the tissue of host plant infected by virulence factor group is damaged Wound and may may be undergone by the mankind that disclosed plant agent composition is applied to the plant infected or otherwise Any adverse effect of plant agent composition disclosed herein is exposed to monitor.Therefore, make in disclosed method or purposes The amount of plant agent composition disclosed herein meets effective quantity standard above, and preferably ornamental plant and agricultural are planted Object, the wild animals and plants that may be contacted with such composition and the mankind have minimum adverse effect or not adversely affect (such as phytotoxicity).
The application of plant agent composition disclosed herein can by effectively generate microbubble as disclosed herein and Effectively exposure seeks any technique of controlled virulence factor to realize.It for example, can be during application by big concentration Any method that gas is introduced into plant agent composition is suitable, because the introducing of such gas can spontaneously form micro- gas Bubble.Suitable technique for applying includes but not limited to spraying, mist formation, atomization, evaporation, scattering, watering, injection, sprinkling and similar Technique.A kind of preferred application process be by manually or mechanically applying, it is described manually or mechanically application by irrigate, spraying, Mist formation, atomization or evaporation.The formation of mist that such application offer is segmented during technique for applying under enough ventilations is to produce Raw microbubble as disclosed herein.The microbubble of the dispersion for the gas being exposed in liquid shows colloidal nature and is referred to as colloid Gas bubble (colloidal gas aphron) (CGA).CGA is different from common gas bubbles, i.e., they include and have any different Shell, the shell includes the surfactant of low concentration.
The microbubble formed with plant agent composition disclosed herein seems to increase the mass transfer of the oxygen in liquid.It does not wish Prestige is fettered by scientific theory, and for this species diversity, there are several possible explanations.First, it is formulated into plant disclosed herein Surfactant in agent composition includes significantly changing nonionic surface active agent and/or the life of the property of bubbling behaviour Object surfactant.Secondly, plant agent combination disclosed herein needs much lower surfactant concentration, for microbubble shape Into.According to it has been shown that surfactant concentration must be close to the critical micelle concentration (CMS) of surfactant system.Herein In disclosed plant agent composition, for used surfactant, microbubble is formed in the CMC less than estimation.This shows Microbubble is surfactant molecule and is more advantageous to the result of the aggregation of the loose packing of molecules of gaseous mass transmission characteristic. Surface comprising less surfactant molecule will be more ventilative than the highly organized micella comprising gas.Do not consider machine System, the trend of the bubble of plant agent composition tissue cluster disclosed herein, aggregation or gas filling provide anti-by increasing The local concentration of object is answered, reduces to be catalyzed and reacts the energy conversion needed for occurring or other the certain mechanism not described still and send out The platform of raw reaction.
In in terms of this embodiment, microbubble disclosed herein has following average diameter：For example, about 5 μm, about 10 μm, about 15 μm, about 20 μm, about 25 μm, about 30 μm, about 40 μm, about 50 μm, about 75 μm, about 100 μm, about 150 μm, about 200 μ M, about 250 μm, about 300 μm, about 350 μm, about 400 μm, about 450 μm, about 500 μm, about 550 μm, about 600 μm, about 650 μm, about 700 μm, about 750 μm, about 800 μm, about 850 μm, about 900 μm, about 950 μm or about 1000 μm.In its other party of this embodiment In face, microbubble disclosed herein has following average diameter：For example, at least 5 μm, at least 10 μm, at least 15 μm, at least 20 μ M, at least 25 μm, at least 30 μm, at least 40 μm, at least 50 μm, at least 100 μm, at least 150 μm, at least 200 μm, at least 250 μ M, at least 300 μm, at least 350 μm, at least 400 μm, at least 450 μm, at least 500 μm, at least 550 μm, at least 600 μm, at least 650 μm, at least 700 μm, at least 750 μm, at least 800 μm, at least 850 μm, at least 900 μm, at least 950 μm or at least 1000 μ m.In in terms of other of this embodiment, microbubble disclosed herein has following average diameter：Such as at most 5 μm, extremely More 10 μm, at most 15 μm, at most 20 μm, at most 25 μm, at most 30 μm, at most 40 μm, at most 50 μm, at most 100 μm, at most 150 μm, at most 200 μm, at most 250 μm, at most 300 μm, at most 350 μm, at most 400 μm, at most 450 μm, at most 500 μm, At most 550 μm, at most 600 μm, at most 650 μm, at most 700 μm, at most 750 μm, at most 800 μm, at most 850 μm, at most 900 μm, at most 950 μm or at most 1000 μm.
In in terms of this embodiment, microbubble disclosed herein has following average diameter：For example, about 5 μm to about 10 μm, about 5 μm to about 15 μm, about 5 μm to about 20 μm, about 5 μm to about 25 μm, about 5 μm to about 310 μm, about 5 μm to about 40 μm, About 5 μm to about 50 μm, about 5 μm to about 75 μm, about 5 μm to about 100 μm, about 10 μm to about 15 μm, about 10 μm to about 20 μm, about 10 μm to about 25 μm, about 10 μm to about 30 μm, about 10 μm to about 40 μm, about 10 μm to about 50 μm, about 10 μm to about 75 μm, about 10 μm To about 100 μm, about 15 μm to about 20 μm, about 15 μm to about 25 μm, about 15 μm to about 30 μm, about 15 μm to about 40 μm, about 15 μm To about 50 μm, about 15 μm to about 75 μm, about 15 μm to about 100 μm, about 20 μm to about 25 μm, about 20 μm to about 30 μm, about 20 μm To about 40 μm, about 20 μm to about 50 μm, about 20 μm to about 75 μm, about 20 μm to about 100 μm, about 25 μm to about 30 μm, about 25 μm To about 40 μm, about 25 μm to about 50 μm, about 25 μm to about 75 μm, about 25 μm to about 100 μm, about 30 μm to about 40 μm, about 30 μm To about 50 μm, about 30 μm to about 75 μm, about 30 μm to about 100 μm, about 40 μm to about 50 μm, about 40 μm to about 75 μm, about 40 μm To about 100 μm, about 50 μm to about 75 μm, about 50 μm to about 100 μm, about 50 μm to about 150 μm, about 50 μm to about 200 μm, about 50 μm to about 250 μm, about 50 μm to about 300 μm, about 50 μm to about 350 μm, about 50 μm to about 400 μm, about 50 μm to about 450 μm, About 50 μm to about 500 μm, about 50 μm to about 550 μm, about 50 μm to about 600 μm, about 50 μm to about 650 μm, about 50 μm to about 700 μm, about 50 μm to about 750 μm, about 50 μm to about 800 μm, about 50 μm to about 850 μm, about 50 μm to about 900 μm, about 50 μm to about 950 μm, about 50 μm to about 1000 μm, about 100 μm to about 150 μm, about 100 μm to about 200 μm, about 100 μm to about 250 μm, about 100 μm to about 300 μm, about 100 μm to about 350 μm, about 100 μm to about 400 μm, about 100 μm to about 450 μm, about 100 μm to about 500 μm, about 100 μm to about 550 μm, about 100 μm to about 600 μm, about 100 μm to about 650 μm, about 100 μm to about 700 μm, about 100 μm to about 750 μm, about 100 μm to about 800 μm, about 100 μm to about 850 μm, about 100 μm to about 900 μm, about 100 μm to about 950 μm, about 100 μm to about 1000 μm, about 150 μm to about 200 μm, about 150 μm to about 250 μm, about 150 μm to about 300 μm, about 150 μm to about 350 μm, about 150 μm to about 400 μm, about 150 μm to about 450 μm, about 150 μm to about 500 μm, about 150 μm to about 550 μm, about 150 μm to about 600 μm, about 150 μm to about 650 μm, about 150 μm to about 700 μm, about 150 μm to about 750 μm, about 150 μm to about 800 μm, about 150 μm to about 850 μm, about 150 μm to about 900 μm, about 150 μm to about 950 μm, about 150 μm to about 1000 μm, about 200 μm to about 250 μm, about 200 μm to about 300 μm, about 200 μm to about 350 μm, about 200 μm to about 400 μm, about 200 μm to about 450 μm, about 200 μm to about 500 μm, about 200 μm to about 550 μm, about 200 μm to about 600 μm, about 200 μm to about 650 μm, about 200 μm to about 700 μm, about 200 μm to about 750 μm, about 200 μm to about 800 μm, about 200 μm to about 850 μm, about 200 μm to about 900 μm, about 200 μm to about 950 μm, about 200 μm to about 1000 μm, about 250 μm to about 300 μm, about 250 μm extremely About 350 μm, about 250 μm to about 400 μm, about 250 μm to about 450 μm, about 250 μm to about 500 μm, about 250 μm to about 550 μm, About 250 μm to about 600 μm, about 250 μm to about 650 μm, about 250 μm to about 700 μm, about 250 μm to about 750 μm, about 250 μm extremely About 800 μm, about 250 μm to about 850 μm, about 250 μm to about 900 μm, about 250 μm to about 950 μm, about 250 μm to about 1000 μm, About 300 μm to about 350 μm, about 300 μm to about 400 μm, about 300 μm to about 450 μm, about 300 μm to about 500 μm, about 300 μm 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The aspect of this specification partly discloses plant.By way of example, plant includes the group of plant or plant Or part, the land such as specific region in meadow, garden or farmland of plant.As text uses, term " plant " refers to belonging to In the organism of any work for the plant kingdom for forming differentiation branch green plants circle.Non-limiting examples include flowering plant, pine and cypress Class plant and other gymnosperms, pteridophyte, lycopods, Anthocerotae, Hepaticae, bryophyte and green alga, but exclude red algae With brown alga, fungi, Archimycetes, bacterium and animal.Vascular plant includes lycopods, equisetales, pteridophyte, gymnosperm (including coniferals) and angiosperm (flowering plant).Scientific for this group includes vascular plant and vascular bundle is planted Object.As text uses, term " flower (flower) " and " flower (bloom) " or " flower (blossom) " they are synonymous, and refer to It is the reproductive ability structure found in angiosperm.As used herein, term " crop plants " refers to generating the plant of crop Object.Non-limiting examples include generating the plant of fruit, seed, nut, cereal, oil, timber and fiber.As used herein , term " crop " refers to the plant product with economic value.Non-limiting examples include generate fruit, seed, nut, Cereal, oil, timber and fiber.
The aspect of this specification partly discloses place (location).Therefore, plant agent composition disclosed herein Various places are advantageously used in, including but not limited to domestic applications, lawn and horticultural applications, agricultural is applied, has tractor-ploughing In kind of application, greenhouse and nursery application, storage products application, professional plant agent application, leaf application, water or submerged applications (underwater or submerged application), soil incorporation application, seedling case processing (seedling box Treatment) (planting treatment application) is applied in application, stalk injection and plantation processing.
It can be included by the plant disease that plant agent composition disclosed herein, method and/or purposes are handled but unlimited In anthracnose, droop, canker (canker), clubroot, plant decay, goiter and tumor, leaf blister (leaf blister), tikka Disease, mildew, mycosis (mold), mosaic virus, rot disease (rot), rust (rust), incrustation (scab), smut and Wilt disease.
Anthracnose or bird's eye spot (bird's-eye spot) refer to one group of plant disease as caused by many fungal species Disease, colletotrichum (Colletotrichum) of the fungal species from many plants for influencing temperature, wet zone, the long spore category of disk (Gloeosporium), small cluster shell category (Glomerella) and Elsinoe (Elsinoe).The developmental branch of common infection (shoots) it is generated in thin, recess, the dish-shaped fructification for being referred to as acervulus (acervuli) with leaf, anthrax fungi Spore.The dead spot of symptom including small recess or in leaf, stem, fruit or the lesion for taking the raised boundary with a variety of colors, And some infection form canker on branch and branch.Although the severity of infection depends on pathogenic dose and infection Kind both, and can out of, simple ugly to dead range, however anthracnose can cause the wilting of tissue, wither and It is dead.Although the disease is found in many plants including grass and annual plant, shade tree such as mulberry tree (sycamore), Chinese ash (ash), Oak Tree and maple are especially sensitive.The example of anthracnose includes but not limited to grape charcoal Subcutaneous ulcer disease (grape anthracnose) (grapevine anthracnose (Elsinoe ampelina)), Japanese anthracnose of kaki (Japanese Persimmon) (the long spore of persimmon disk (Gloeosporium kaki)), Strawberry anthracnose (Glomerella cingulata), cucurbit Anthracnose (gourd anthracnose) (cucumber anthracnose (Colletotrichum lagenarium)), kidney bean anthracnose (Colletotrichum lindemthianum), anthracnose of tea (Colletotrichum theaesinensis) and cigarette Careless anthracnose (Colletotrichum tabacum).
Droop refers to one group of plant disease as caused by the kind of many fungies and bacterium, is characterized in planting in symptom The rapid and serious chlorosis of object tissue such as leaf, branch, withe or floral organ, flavescence become palm fibre, are spottiness, withered and right It is dead afterwards.Both fungi and bacterium droop most frequently occur under cold wet condition, and usually attack plant tender shoots and The tissue mushroomed out at other initial stages.Economically most important plant is easy infection to one or more of droops, packet Include tomato, potato and apple and many other ornamental species.Droop is often with its pathogenic dose name, such as anthrax-bacilus Belong to droop to name with fungi Colletotrichum capsici (Colletotrichum capsici), and Phytophthora droop is with water mo(u)ld Tobacco black shank bacterium (Phytophthora parasitica) is named.It is (early that the example of droop includes but not limited to alternaria Phase) droop (tomato early blight bacterium (Alternaria solani)), (Fusarium graminearum, potato are withered for Fusarium droop Wither germ (F.avenaceum), fusarium culmorum, micro- spore category pinta (Microdochium nivale)), Phytophthora (after Phase) droop (phytophthora infestans category (Phytophthora infestan)), rhizoctonia seedling droop (Rhizoctonia solani Kuhn (Rhizoctonia solani))；Rhizoctonia air droop (rhizoctonia aerial blight) (miliary damping-off Bacterium), core coral Pseudomonas snow blight (typhula snow blight) (core coral Pseudomonas (Typhula sp.)), apple flower droop (apple blossom blight) (chain sclerotinia sclerotiorum (Monilinia mali)), bacterium rice leaf blight (bacterial Rice leaf blight) (rice leaf spot bacteria (Xanthomonas oryzae)), chestnut droop (chestnut Blight) fire blight (the erwinia amylovora of (Cryphonectria Parasitica (Cryphonectria parasitica)), a kind of fruit, such as apple, pear, etc. (Erwinia amylovora)), gray blight (gray blight) (intend Pestalotia (Pestalotiopsis sp.)), leaf Blight (chrysanthemum leaf spot blight (Septoria chrysanthemi-indici), Rhizoctonia solani Kuhn (Thanatephorus Cucumeris)), net blister sick (net blister blight) (tea net cake germ (Exobasidium reticulatum)), Banded sclerotial blight (sheath blight) (Rhizoctonia solani Kuhn), southern peanut droop (Southern peanut blight) are (neat Pyrenomycetes (Sclerotium rolfsii)), southern corn leaf blight (Southern corn leaf blight) (different rotation spore Chamber bacterium (Cochliobolus heterostrophus), southern corn leaf blight (Bipolaris maydis)), soybean pod and stem Droop (soybean pod and stem blight) (soybean stain germ (Diaporthe phaseolorum )) and liana droop (Mycosphaerella melonis) var.sojae.
Canker refers to one group of common and extensive plant disease, the plant as caused by the kind of many fungies and bacterium Object disease mainly occurs on many xylophytas.Symptom includes the circle on stem (branch), withe, branch (limb) or limb Shape to irregular (round-to-irregular), recess, swelling, it is flattening or rupture, decoloration and top drying Dead region.Canker can expand and fetter withe or branch, kill the branches and leaves other than branches and leaves.Canker can be in structure Plant is weakened until plant crushes in wind or during ice storm.Canker also slows down the normal of wound and more merges into decay of wood Or wilt and license is provided, generate fungi and other biological body.They are by cold or arid threat, insect damage, nutrient imbalance, line It is most common on worm or the plant of root rot (root rot) reduction.Canker can be classified as the sick (sickle of annual canker Spore Pseudomonas canker (Fusarium canker)), perennial canker disease it is (Nectria canker (Nectria canker), curved The poly- shell category canker of spore (Eutypella canker)) and diffusivity canker (Botryosphaeria canker (Botryosphaeria canker), Phytophthora top dry (Phytophthora dieback), Cytospora canker (Cytospora canker)).The example of canker includes but not limited to fungi canker such as apple tree canker, and (apple tree is rotten Rotten germ (Valsa ceratosperma)), Botryosphaeria canker (Botryosphaeria canker), Cytospora Poly- shell category (Eutypella) (maple) canker of canker (Cytospora canker), curved spore, Nectria canker (Nectria canker), Phytophthora top dry, urnu gentian herb category (Urnula) (Oak Tree) canker；And Bacterial Canker Disease is such as Erwinia canker, pseudomonad canker (pseudomonas syringae) and xanthomonas canker (Xanthomonas canker)。
Clubroot refers to influencing one group of plant disease of the member of cabbage family, what the plant disease was given birth to by soil Fungi plasmodiophora brassicae (Plasmodiophora brassicae) causes and is characterized in often cracking and corruption in symptom Rotten deformity and (rodlike) root of deformation.Therefore, plant is difficult to suitably absorb water and nutrients.Plant may slowly give birth to It grows and wilts during the sweltering heat of one day；Plant often restores during nice and cool night.External leaf eventually becomes yellow Or brown.Clubroot will reduce yield and can cause total vegetative loss.
Plant decay disease refers to influence seed and one group of plant disease of new seedling, and is caused by several fungies, The fungi includes Fusarium, Phytophthora, pythium (Pythium) and Rhizoctonia (Rhizoctonia) including vertical withered Rhizoctonia, and be characterized in being in and less than the stem of soil surface and rotting for root tissue in symptom.In majority of case Under, the plant of infection will germinate and start to sprout, but in several days, that they become to be waterlogged and paste, fall in base Portion is simultaneously dead.
Gall matosis refers to one group of plant disease as caused by fungi, bacterium, virus and nematode and certain insects, It is characterized in abnormal, local growth (outgrowth) or the swelling of plant tissue in symptom.Goiter and tumor infects and kills rattan This plant, ornamental flower, fruit tree, brambles (bramble), shade tree and vegetables.Other plant disease also develops into goiter and tumor For the second fruiting of infection, including cedar apple rust (cedar-apple rust)；Clubroot and corn smut.Goiter and tumor The example of disease includes but not limited to plant black knot (Dibotryon morbosum) and crown gall (Agrobacterium tumefaciems (Agrobacterium tumefaciens))。
The leaf blister for being also known as leaf curl refers to one group of many xylophytas and pteridophyte in world wide Plant disease, and caused by the fungi of Exoascus, and be characterized in leaf distorting, crispaturaing in symptom.Cold After moist weather, at germination, leaf becomes swelling, wrinkle and distortion, has yellow, red on the upper surface of leaf Color, brown, whitens or the blister of grey at purple, is recessed on the lower surface with grey.Such leaf is usually dead and falls in early days It falls, makes plant weak.Fruit can be generally reduced by the disleave of leaf blister to produce, and can even kill tree.Leaf blotch Disease influence peach, nectarine tree, Japanese plum, apricot (almond), Chinese bush cherry tree (amelanchier tree), almond tree (apricot), Birch, cherry tree, cherry bay tree (cherry laurel tree), California horse chestnut (California Buckeye tree), alder tree, Oak Tree and willow.Mycotic example includes but not limited to leaf curl (Taphrina deforman)。
Leaf spot refers to one group of plant disease as caused by a large amount of fungies and bacterium, and plant is characterized in symptom Leaf on spot.The plant of infection has the spot of brown or black water saturates on leaf, has sometimes usually in size Upper uniform yellow halo.Spot will expand and run together under humid conditions.Under the dry condition, spot has Appearance (speckled appearance) with spot.As spot becomes more, whole leaves may turn yellow, wither and fall. Fungal leaf spots disease is included from following kind：Alternaria, starlet shield soot category (Asterina), star shield soot category (Asterinella), cercospora (Cercospora), Cercosporalla Cercosporella), rotation spore Pseudomonas (Cochliobolus), Corynespora (Corynespora), bivalve belong to (Diplocarpon), star shell spore category (Diplotheca), cercospora glue category (Gloeocercospora), small cluster shell Pseudomonas, Gnomonia (Gnomonia), Phomopsis Pseudomonas (Phomopsis), nuclear cavity Pseudomonas (Pyrenophora), splits shield Pseudomonas at flat shell Mildew Pseudomonas (Placosphaeria) (Schizothyrium), pyrenomycetes category (Sclerotinia), Septoria (Septoria) and Ji Ci Mildew Pseudomonas (Stigmea).Fungal leaf spots disease is attacked lettuce and can also be occurred on holder platymiscium and other vegetables, the vegetables packet Include such as cabbage, cauliflower (cauliflower), Chinese cabbage, broccoli, brussels sprout, kohlrabi, collard, grassland Cyanines and turnip (rutabaga).Fungal leaf spots disease also will infection strawberry plants and white poplar and willow.Leaf spot Problem will be caused.Fungal leaf spots disease includes the kind from pseudomonas.Bacterial leaf spot particularly attack Prunus family into Member's (drupe, including cherry, plum, almond, apricot peach kernel (apricot) and peach), Prunus family are special to bacterial leaf spot Insensitive.Fruit is likely to occur spottiness or the brown area with recess.Bacterial leaf spot will also infection tomato and capsicum Crop and certain annual and perennial flowering plant, the flowering plant include rose, fish pelargonium, Zinnia, purple cone Flower and Thunbergia alata.The example of wilting leaf spot includes but not limited to alternaria leaf spot (alternaric bacteria (Alternaria Alternata), Alternaria brassicicola (Alternaria brassicicola), Alternaria japonica (Alternaria Japonica)), black spot (alternaric bacteria, Diplocarponrosae (Diplocarpon rosae)), brown spot (Alternarialongipes (Alternaria longipes), Hemintho-sporum leaf spot of rice plants bacterium (Cochliobolus miyabeanus), Phomopisis vexans bacterium (Phomopsis vexans), soybean Septoria musiva (Septoria glycines)), cercospora leaf spot (beet tail spore bacterium (Cercospora beticola)), first pinta (dollar spot) is (the same as fruit stone cup fungi (Sclerotinia Homeocarpa)), early stage leaf spot (cercospora black spot of peanut bacterium (Cercospora personata)), Frogeye Leaf Spot (frog Eye leaf spot) (Germ To Soybean Frogeye Leaf Spot (Cercospora sojina)), gray leaf spot (gray leaf spot) (jade Rice Cercospora Sojina Hara (Cercospora zeae-maydis)), late period leaf spot (peanut Cercospora bacteria (Cercospora Arachidicola)), leaf spot (persimmon angular leaf spot (Cercospora kaki), leaf spot of kaki bacterium (Mycosphaerella Nawae)), brown spot (tan spot) (helminthosporium yellow leaf blotch of wheat (Pyrenophora tritici-repentis)), Leucoplakia (Cercosporella brassicae), zonate spot (target spot) (cucumber stick spore leaf spot fungi (Corynespora )) and mulberry zonate spot (zonate leaf spot) (sorghum cercospora glue (Gloeocercospora cassiicola sorghi))。
Mildew refers to that, by many fungus-caused one group of plant diseases, the feature in symptom is that typically in the upper of leaf White, grey on surface or lower surface, the powdered growth with blue or purple.Small stain occurs and generates to pass through wind To infect the spore of new plant.Leaf will become brown, and when mildew is extensive, and leaf often wilted, withers and in early stage Death, fruit precocious and quality and flavor with difference.Seedling may wilt and collapse.Hundreds of trees, shrub, liana are planted Object, flowers, veterinary antibiotics, grass, field crop, ornamental plant, shrub fruit and weeds may be influenced by mildew.Mildew Example include but not limited to downy mildew (basidiospore Pseudomonas (Basidiophora spp.), lettuce Bremia (B.lactucae) and other Bremias (Bremia spp.), wheat powdery mildew (Erysiphe graminis), grape Downy mildew (Plasmopara viticola), Podosphaera (Podosphaera), green onion downy mildew (Peronospora Destructor), Peronospora parasitic bacterium (Peronospora parasitica), right angle shield-fern downy mildew (Peronospora Sparsa), tobacco downy mildew (Peronospora tabacina) and other Peronospora (Peronospora spp.), phytophthoras Category, shaft peronospora (Plasmopara spp.), bacterium of downy mildew of cucumber (Pseudoperonospora cubensis) and other Pseudoperonospora and Sclerospora (Sclerospora spp.) and white powder mildew (powdery mildew) (powdery mildew of cucumber (Erysiphe cichoracearum), wheat powdery mildew (Erysiphe graminis), pea powdery mildew (Erysiphe ) and other Erysiphe (Erysiphe spp.), Microsphaera (Microsphaera), Phyllactinias pisi (Phyllactinia), white Podosphaera (Podosphaera leucotricha) and other Erysiphes (Podosphaera spp.), cucumber powdery mildew fungus (Sphaerotheca fuliginea), the single softgel shell bacterium of rule grass (Sphaerotheca humuli), rose list softgel shell bacterium (Sphaerotheca pannosa) and other Sphaerothecas (Sphaerotheca spp.) and uncinula necator (Uncinula necator) and other genus erysiphes (Uncinula spp.))。
Mycosis (mold) refers to, by several fungus-caused one group of plant disease, being characterized in infecting in symptom Plant part surface on powdered or wool-like appearance.Mycosis attacks extensive plant, including cereal, herbage (forage grass) and sod grass (turf grass), particularly it is moist, feel nice and cool to mild weather.Mycotic reality Example includes but not limited to grey mycosis (Botrytis cinerea), leaf mycete sick (Cladosporium fulvum), pink colour Avenge mycosis (Fusarium nivale) and snow mycosis (Typhula itoana).
Mosaic virus refers to one group of plant disease as caused by plant virus, and is characterized in having in symptom Several malnourished appearances.Leaf has the striped of yellow or spot, leaf wrinkly or curling, stunted growth and subtracts Small yield.The fruit of infection is presented mottling and forms increased " wart " area.Mosaic virus influences many plants, including rose Rare, beans, tobacco, tomato, potato and capsicum.The example of mosaic disease seed culture of viruses includes but not limited to aphidophilus betae, plum Flower poxvirus (Potyvirus spp.), tobacco mosaic virus (TMV) (Tobamovirus (Tobamovirus spp.)), cassava Mosaic virus (Geminiviruses (Begomovirus spp.)), cowpea mosaic virus, cucumber mosaic virus, Alfalfa mosaic virus, broomcorn millet floral leaf satellite virus (panicum mosaic satellite virus), pumpkin mosaic virus, Radix Curcumae Fragrant broken color viral (tulip breaking virus) and small zucchini yellow mosaic virus (zucchini yellow mosaic virus)。
The rot disease of also referred to as corrupt (decay) refers to any of the kind of the fungi and bacterium given birth to by hundreds of soil Caused one group of plant disease is characterized in that plant decomposes and degenerates (putrefaction) in symptom.It decomposes and degenerates It is due to the corruption of root, stem, timber, flower and/or fruit.Corruption can be it is hard and dry, soft and viscous, aqueous, It is paste or sticky, and any plant part may be influenced.Many rot diseases are in the fruit of storage, root, bulb or stem tuber In be very active.Certain rotten diseases cause leaf corrupt, but those symptoms trend is described as leaf spot and droop.It is rotten The example of rotten disease include but not limited to Aphanomyces (Aphanomyces) root decomposing disease (Aphanomyces cochlioides), Phomopsis Pseudomonas rot disease (Phomopsis Pseudomonas), Phytophthora cactorum bacterium (Phytophtora cactorum), soyabean phytophthora (Phytophthora sojae) and other Phytophthoras), pyrenomycetes category rot disease (sclerotinite (Sclerotinia Sclerotiorum)), bitter rot disease (bitter rot) (sharp spore anthrax-bacilus (Colletotrichum acutatum)), black corruption Rotten disease (black rot) (grape Guignardia (Guignardia bidwellii)), tan rot (Monilinia fructicola (Monilinia fructicola)), hat rot disease (crown rot) (Phytophthora cactorum bacterium (Phytophtora cactorum)), Fruit decomposing disease (penicillium digitatum (Penicillium digitatum), penicillium italicum (P.italicum)), mushroom are rotten Rotten sick (mushroom rot), powder rot disease (pink rot) (red corruption phytophthora (Phytophthora Erythroseptica)), Ripe rot (ripe rot) (GLOMERFLLA CINGULATA bacterium (Glomerella cingulata)), root decomposing disease (silk capsule mould (Aphanomyces spp.), close ring bacterium, easily die a glass agaric (Clitocybe tabescens), Fusarium, rotten mold genus, Phytophthora, Rhizoctonia solani Kuhn), stem rot is rotten and wooden rot disease.
Rust refers to that, by being more than 5,000 kind of fungus-caused one group of plant disease is characterized in conduct in symptom The Huang that coating (coating) on leaf, sprout (young shoot) and fruit in thousands of kinds of economically important plants occurs Color, orange, red, rust, brown or black powder warts.Plant growth and productivity are usually reduced；Certain plants is withered It withers and withered.During the life cycle of rust fungi, rust fungi parasitize a kind of plant (single it is parasitic or monoecism Rust) or two different kinds (heteroecism rust).Single parasitism rust include attack asparagus, beans, chrysanthemum, coffee, The rust of hollyhock, toad's-mouth and sugarcane.Heteroecism rust includes attack cereal, grass, Chinese juniper (juniper), fir, poplar Tree, apple tree, Japanese quince (Japanese quince), hawthorn, rose, currant and gooseberry heteroecism rust.Rust The example of disease includes but not limited to asparagus rust, big wheat rust (Stripe Rust (Puccinia striiformis), stem rust germ (Puccinia graminis), barley aecidium (P.hordei), black stem rust (stem rust germ), deodar rust of apple (Gymnosporangium yamadai) (cedar apple rust), more born of the same parents' Rusts (Phragmidium spp.), grape rust (Phakopsora Ampelopsidis), leaf rust (leaf rust bacterium (Puccinia triticina triticia)), rust of pear (pear gymnosporangium (Gymnosporangium haraeanum)), southern rust (Southern rust) (stripe rust of wheat Puccinia Polvsora), soybean rust (phakopsora pachyrhizi), item (Huang) rust (Puccinia striiformis Tritici), scallion rust (Puccinia allii), wheat rust (Stripe Rust, stem rust germ, concealment handle rust (P.recondita)), white blister (Albugo (Albugo spp.), hole handle rust (Puccinia horiana)) and white Pine-cluster cups (Cronartium ribicola (Cronartium ribicola)).
Incrustation refer to one group of plant disease as caused by several fungies and bacterium, be characterized in symptom fruit, Hardening, undue growth on stem tuber, leaf or stem and the tissue (shelly-shaped lesion) ruptured sometimes.The leaf of impacted plant It in incipient wilting and may fall.Incrustation often influences apple, crabapple (crab apple), cereal, cucumber, peach, U.S. State hickory nut, photinia glabra (photinis), potato and fiery spine (pyracantha) trees or plant.Fruit incrustation can Can be the main problem about apple and peach, and potato is especially sensitive to common incrustation.The example packet of incrustation It includes but is not limited to apple incrustation (venturia inaequalis (Venturia inaequalis)), citrus incrustation (citrus scab sore germ (Elsinoe fawcetti)), common incrustation (streptomyces scabies (Streptomyces scabies)), peach incrustation (thermophilic fruit Cladosporium sp (Cladosporium carpophilum)), incrustation (pear cucumerinum (Venturia nashicola), pears are black Star bacterium (V.pirina)), powdery potato incrustation (powdery potato scab) (spongospora subterranea (Spongospora Subterranean f.sp.subterranea)) and white incrustation (white scab) (Elsinoeleucospila (Elsinoe leucospila))。
Smut refers to, by fungus-caused one group of plant disease, being characterized in being referred to as sporangium in symptom Group (sori) cigarette ash sample agglomerate (sootlike mass) in accumulate fungal spore, the fungal spore seed, leaf, stem, It is formed in blister in flower part and bulb.Sporangiorus is usually broken to the black powder easily disperseed by wind.It is many Smut fungi enters embryo or small plants, whole body development, and and if only if plant is presented close to during maturation in outside.Other are black Powder disease is local, the tissue of active infections growth.Smut is in grass, cereal and corn (corn) (corn (maize)), sugarcane With it is most common on sorghum.The example of smut includes but not limited to barley smut (Ustilago nuda), corn smut (Ustilago maydis (Ustilago maydis), ustilago zeae (U.zeae)), loose smut (loose smut) are (small Wheat ustilago (Ustilago tritici)) and stinking smut (T. caries (Tilletia caries)。
Wilt disease refers to one group of plant disease as caused by many fungies and bacterium, and is characterized in forever in symptom Long dwarfing is wilted and withered, is often followed by the death of all or part of plants.The fungi of wilting is caused to include from sickle The kind that spore Pseudomonas and Verticillium dahliae belong to, and cause the bacterium of wilting from corynebacterium, Erwinia (Erwinia), false list Born of the same parents Pseudomonas and the kind of xanthomonas (Xanthomonas).Wilt disease influence more than 500 kinds of tree, shrub, liana, Flower, household plant (house plant), veterinary antibiotics, field crop and weeds.The example of wilt disease includes but not limited to Fusarium wilt disease (Fusarium oxysporum), Oak Tree wilt disease (Ceratocystis fagacearum (Bretz) Hunt (Ceratocystis fagacearum), this Family name's wilt disease (Stewart's wilt) and verticillium wilt are (black and white verticillium sp (Verticillium alboatrum), big beautiful Verticillium dahliae (V.dahliae)).
Its group can be included by the virulence factor that plant agent composition disclosed herein, method and/or purposes control But it is not limited to virus, bacterium, fungi and nematode.In addition, all developing stage can pass through plant agent group disclosed herein Close object, method and/or purposes control, including but not limited to ovum, larva, pupa (nymphal), childhood, pupa (pupal) and into Year.
Plant agent composition disclosed herein, method and/or purposes can control belong to Prokaryota, mycota and The group of the virulence factor of Nematoda.In embodiments, plant agent composition disclosed herein, method and/or purposes can be with Control belongs to the group of the virulence factor (being referred to as bacterium virulence factor) in bacterium domain (Domain Bacteria) and cyanobacteria domain. In in terms of this embodiment, plant agent composition disclosed herein, method and/or purposes can control the cause for belonging to following The group of cause of disease：Acidfast bacilli door (Phylum Acidobacteria), actinomyces door (Actinobacteria), production water bacterium door (Aquificae), Armatimonadetes (armoring bacterium door), Bacteroidetes (Bacteroidetes), thermophilic silk bacterium door (Caldiserica), Chlamydia door (Chlamydiae), green bacterium door (Chlorobi), green curved bacterium door (Chloroflexi), production Jin Junmen (Chrysiogenetes), cyanobacteria, deferrization bacillus door (Deferribacteres), abnormal cocci-Thermus door (Deinococcus-Thermus), net group's bacterium door (Dictyoglomi), Elusimicrobia, Filamentous bacillus door (Fibrobacter), Firmicutes (Firmicutes), fusiform bacilarmature door (Fusobacteria), bud monad door (Gemmatimonadetes), viscose ball bacteria door (Lentisphaerae), nitrification spirillum door (Nitrospira), floating mould Bacterium door (Planctomycetes), Proteobacteria (Proteobacteria), conveyor screw, mutual bacteria door (Synergistetes), Without wall bacterium door, thermally desulfurizing bacillus door (Thermodesulfobacteria), hot germ door (Thermomicrobia), thermobacillus Door (Thermotogae) or wart germ door (Verrucomicrobia).Particularly belonging to for bacterium virulence factor is non-exhaustive clear It is single to include but not limited to Agrobacterium (Agrobacterium), corynebacteria, chestnut epidemic disease Pseudomonas (Cryphonectria), Ou Wenshi Pseudomonas, Penicillium, pseudomonas, streptomyces and xanthomonas (Xanthomonas).The spy of bacterium virulence factor Other kind of non-exhaustive inventory includes but not limited to Agrobacterium tumefaciems (Agrobacterium tumefaciens), parasitic chestnut Vaccine (Cryphonectria parasitica), chestnut epidemic disease category (Cryphonectria), erwinia amylovora, penicillium digitatum, meaning Big profit Penicillium notatum (P.italicum), pseudomonas syringae, streptomyces scabies and ecru monad (Xanthomonas oryzae)。
In another embodiment, plant agent composition disclosed herein, method and/or purposes can control and belong to disease The group of the virulence factor of malicious mesh (Order Virales).It is non-exhaustive clear to be referred to as particularly belonging to for the viral pathogenesis factor It is single to include but not limited to Begomovirus (Begomovirus), Potyvirus group and tobacco mosaic virus (TMV).
In another embodiment, plant agent composition disclosed herein, method and/or purposes can control belong to Under virulence factor group：Ascomycota (Division Ascomycota), is partly known Basidiomycota (Basidiomycota) Bacterium door (Deuteromycota) or Zygomycota.The non-exhaustive inventory particularly belonged to for being referred to as the fungal attack factor includes But it is not limited to Albugo (Albugo), alternaria, Aphanomyces (Aphanomyces), halimasch (Armillaria), small Star shield soot category (Asterina), star shield soot category, basidiospore Pseudomonas, Bipolaris, Botryosphaeria (Botryosphaeria), Botrytis, Bremia, long beak shell category (Ceratocystis), cercospora, Cercosporalla (Cercosporella), branch Spore bacterium (Cladosporium), Clitocybe (Clitocybe), rotation spore Pseudomonas, colletotrichum, Corynespora, column Rust (Cronartium), Cytospora (Cytospora), Beancurd sheet shell Pseudomonas (Diaporthe), double Portugal's chamber Pseudomonas (Dibotryon), Bivalve belongs to (Diplocarpon), star shell spore category (Diplotheca), Elsinoe, Erysiphe, the poly- shell category of curved spore (Eutypella), Exobasidium (Exobasidium), Fusarium, cercospora glue category (Gloeocercospora), the long spore category of disk (Gloeosporium), small cluster shell Pseudomonas, Gnomonia (Gnomonia), ball seat Pseudomonas (Guignardia), glue Rust (Gymnosporangium), micro- tubercle Pseudomonas (Microdochium), Microsphaera, chain sclerotinia sclerotiorum belong (Monilinia), ball Chamber Pseudomonas (Mycosphaerella), clump red shell Pseudomonas (Nectria), intend Pestalotia at downy mildew Pseudomonas (Peronospora) (Pestalotiopsis), Phakopsora (Phakopsora), Phomopsis Pseudomonas, more born of the same parents' Rusts (Phragmidium), ball Needle shell category (Phyllactinia), Phytophthora, flat shell Mildew Pseudomonas (Placosphaeria), knee Pseudomonas (Plasmodiophora), Plasmopara (Plasmopara), single softgel shell bacterium, Pseudoperonospora, Puccinia, Puccinia, corruption Mould category, Rhizoctonia split shield Pseudomonas (Schizothyrium), Sclerospora (Sclerospora), pyrenomycetes category, Septoria (Septoria), (Spongospora), Ci Mildew Pseudomonas, dies lead fungi category at Exoascus for Sphaerotheca, powder scab Pseudomonas (Thanatephorus), Tilletia (Tilletia), core coral Pseudomonas (Typhula), powdery mildew Pseudomonas (Uncinula), Urnu gentian herb category (Urnula), Ustilago (Ustilago), black skin shell Pseudomonas (Valsa), Venturia (Venturia) And Verticillium dahliae category.Special kind of non-exhaustive inventory of the fungal attack factor includes but not limited to alternaric bacteria, wild cabbage chain Lattice spore bacterium, Alternaria japonica (Alternaria japonica), Alternarialongipes bacterium, tomato early blight bacterium, spiral case shape silk capsule Mould (Aphanomyces cochlioides), close ring bacterium, southern corn leaf blight (Bipolaris maydis), the pathogen of Botrytis cinerea (Botrytis cinerea), lettuce disk stalk mould, Ceratocystis fagacearum (Bretz) Hunt, peanut Cercospora bacteria, beet tail spore bacterium, persimmon angular leaf spot bacterium (C.kaki), cercospora black spot of peanut bacterium, Germ To Soybean Frogeye Leaf Spot, Gray Leaf Spot Pathogen, black spot of cabbage (Cercosporella Brassicae), cladosporium carpophilum bacterium, leaf muld of tomato (C.fulvum), easy die a glass agaric, southern corn leaf blight, rice flax Pinta bacterium, sharp spore anthrax-bacilus, capsicum anthrax-bacilus (C.capsici), cucurbit anthrax-bacilus (C.lagenarium), Kidney bean anthrax-bacilus (C.lindemthianum), tea anthrax-bacilus (C.theaesinensis), tobacco anthrax-bacilus (C.tabacum), cucumber stick spore leaf Pinta bacterium, Cronartium ribicola, soybean stem canker (Diaporthe phaseolorum), Prunus melanocarcinoma germ (Dibotryon morbosum), Diplocarponrosae, grape Elsinochrome (Elsinoe ampelina), citrus scab sore germ, Elsinoeleucospila (E.leucospila), cucumber powdery mildew's pathogen (Erysiphe cichoracearum), millet Powdery Mildew (E.graminis), powdery mildew of pea bacterium (E.pisi), tea net cake germ, potato wilt bacterium, fusarium culmorum, cereal sickle Spore bacterium, Fusarium oxysporum, pink colour snow mycosis (F.nivale), sorghum cercospora glue (Gloeocercospora sorghi), persimmon The long spore of disk (Gloeosporium kaki), Strawberry anthracnose Glomerella cingulata), grape Guignardia (Guignardia bidwellii), pear gymnosporangium, micro- spore category pinta (Microdochium nivale), drupe chain nuclear disk Bacterium (Monilinia laxa), a kind of fruit, such as apple, pear, etc. brown rot germ (M.fructigena), Monilinia fructicola, chain sclerotinia sclerotiorum (M.mali), rattan This plant droop (Mycosphaerella melonis), leaf spot of kaki bacterium (M.nawae), green onion downy mildew (Peronospora Destructor), phytophthora parasitic (P.Parasitica), right angle shield-fern downy mildew (P.Sparsa), tobacco downy mildew (P.tabacina), grape layer rest fungus (Phakopsora ampelopsidis), phakopsora pachyrhizi sydow (P.pachyrhizi), juniper Plan stem point bacterium (Phomopsis juniperovora), Phomopisis vexans bacterium, Phytophthora cactorum bacterium (Phytophtora cactorum), red corruption Phytophthora (P.erythroseptica), phytophthora infestans, phytophthora parasitic (P.parasitica), soyabean phytophthora, wild cabbage Plasmodiophora brassicae (Plasmodiophora brassicae), P. viticola, podosphaera leucotricha, helminthosporium yellow leaf blotch of wheat (Pyrenophora tritici-repentis), bacterium of downy mildew of cucumber (Pseudoperonospora cubensis), shallot Rust (Puccinia allii), stem rust germ, big wheat rust (P.hordei), hole handle rust (P.horiana), wheat item rust Sick (P.polvsora), concealment handle rust (P.recondita), Stripe Rust, leaf rust bacterium, Rhizoctonia solani Kuhn, with fruit stone disk Bacterium (Sclerotinia homeocarpa), Sclerotium rolfsii, sclerotinite, chrysanthemum leaf spot blight (Septoria Chrysanthemi-indici), soybean Septoria musiva, spotted wilt of tomato (S.lycopersici), cucumber powdery mildew fungus (Sphaerotheca fuliginea), the single softgel shell bacterium (S.Humuli) of rule grass, rose list softgel shell bacterium (S.pannosa), Ma Ling Potato powder scab bacterium (Spongospora subterranean), lopsided external capsule bacterium (Taphrina deformans), rice sheath blight disease Bacterium (Thanatephorus cucumeris), T. caries (Tilletia caries), snow mycosis (Typhula itoana), uncinula necator (Uncinula necator), ustilago zeae, barley smut (U.nuda), Ustilago tritici (U.Tritici), ustilago zeae (U.zeae), apple rot pathogen, venturia inaequalis, the black star of pears Germ, Pear scab (V.pirina), black and white verticillium sp and verticillium dahliae (V.dahlia).
In another embodiment, plant agent composition disclosed herein, method and/or purposes can control and belong to line The group of the virulence factor of worm door (Nematomorpha).It is referred to as the non-exhaustive inventory packet particularly belonged to of nematode virulence factor It includes but is not limited to Aphelenchoides (Aphelenchoides), thorn Turbatrix (Belonolaimus), small loop wire Eimeria (Criconemella), Dirofilaria (Dirofilaria), Ditylenchus (Ditylenchus), Heterodera (Heterodera), Hirschmanniella (Hirschmanniella), tie category (Hoplolaimus), Meloidogyne (Meloidogyne), Onchocerca (Onchocerca), Pratylenchus (Pratylenchus), perforation line Eimeria (Radopholus) and kidney shape turbatrix (Rotylenchulus).Special kind of nematode virulence factor it is non-exhaustive clear It is single to include but not limited to heart worm (Dirofilaria immitis), corn cyst roundworm (Heterodera zeae), root Tie lines worm (Meloidogyne incognita), javanese root knot nematode (Meloidogyne javanica), Onchocerca caecutiens (Onchocerca volvulus), radopholus similes thorne (Radopholus similis) and kidney worm (Rotylenchulus reniformis)。
Plant agent composition described herein, method and purposes will most unlikely damage mammal or environment, and be Non-plant toxicity and can be safely applied to economically valuable plant or crop.In addition, plant described herein Agent composition, method and purposes can indoors with outdoor application and will not soften, dissolve or otherwise adversely Influence processed surface.Finally, virulence factor will not generate plant agent composition described herein, method and purposes anti- Property.
The aspect of this specification can also be described as follows：
1. it is a kind of control plant disease virulence factor method, the method includes it is following, be substantially made up of Or it is made up of：A effective amount of plant agent composition is applied to the one or more of plants infected by the virulence factor And/or by a effective amount of plant agent composition application in a manner that wherein virulence factor will be exposed to the plant agent composition To one or more places, wherein the application of the plant agent composition causes to seeking described in controlled plant disease The adverse effect of virulence factor, wherein the composition is included hereinafter, being substantially made up of or being made up of：Through processing Fermentation microorganism supernatant and one or more of nonionic surface active agent, wherein the composition does not have any work Property enzyme or work bacterium, and wherein described composition have at most 5.0 pH.
2. a kind of method for increasing plant growth and/or crop production, the method includes it is following, substantially by with the following group Into or be made up of：A effective amount of plant agent composition is applied to one or more of plants and/or by a effective amount of plant Agent composition, which is applied to wherein described plant agent composition, will be exposed to one of one or more of plant or more Multiple places, wherein the application of the plant agent composition leads to the improved absorption by root hair, it is improved to pass through xylem Xylem sap flowing and the improved photosynthate flowing in bast, increased water, minerals and come from The absorption of other nutrients of soil, increased capillarity and/or hydrostatic pressing and/or increasedization in xylem Close object and energy synthesis and/or one or more of groups of the flowing of blocking xylem sap and/or photosynthate flowing Point destruction, wherein the composition include it is following, be substantially made up of or be made up of：Processed fermentation it is micro- Biological supernatant and one or more of nonionic surface active agent, wherein the composition does not have any organized enzyme or work Bacterium, and wherein described composition has at most 5.0 pH.
Kept or the method for efficiency that improves the irrigation system 3. a kind of, the method includes it is following, substantially by with the following group Into or be made up of：A effective amount of plant agent composition is applied to one in the pipeline network of the irrigation system or more Multiple pipelines, wherein the application of the plant agent composition causes to stop the one of one or more pipeline networks of irrigation system Kind or more kind component suitable removing, wherein the composition include it is following, be substantially made up of or by with the following group Into：The microorganism supernatant of processed fermentation and one or more of nonionic surface active agent, wherein the composition There is no the bacterium of any organized enzyme or work, and wherein described composition has at most 5.0 pH.
4. a effective amount of plant agent composition is used to control the purposes of plant disease, wherein the composition include it is following, It is substantially made up of or is made up of：The microorganism supernatant and one or more of processed fermentation are non-ionic Surfactant, wherein the composition does not have the bacterium of any organized enzyme or work, and wherein described composition has at most 5.0 pH.
5. a effective amount of plant agent composition is used to increase the purposes of plant growth and/or crop production, wherein described group Close object include it is following, be substantially made up of or be made up of：The microorganism supernatant of processed fermentation and it is a kind of or More kinds of nonionic surface active agent, wherein the composition does not have the bacterium of any organized enzyme or work, and wherein described Composition has at most 5.0 pH.
6. a effective amount of plant agent composition is used for the purposes of efficiency for keeping or improving the irrigation system, wherein the combination Object include it is following, be substantially made up of or be made up of：The microorganism supernatant of processed fermentation and a kind of or more A variety of nonionic surface active agent, wherein the composition does not have the bacterium of any organized enzyme or work, and wherein described group Closing object has at most 5.0 pH.
7. the method according to embodiment 1-3 or the purposes according to embodiment 4-6, wherein described through processing Fermentation yeast supernatants of the microorganism supernatant from fermentation, fermentation bacterial supernatant, fermentation mould supernatant or Any combination thereof.
8. method or purposes according to embodiment 6, wherein the yeast supernatants of the fermentation are by belonging to following The yeast species of category generate：Brettanomyce Pseudomonas, Candida, intend Weir Blastocystis, the black powder Blastocystis of sporangiocyst line, Dbaly yeast Pseudomonas, moral carat Blastocystis, Fusarium, geotrichum sp category, her Sa Blastocystis, Kazakhstan's saccharomycete Category, Ke Leke Blastocystis, Kluyveromyces, lecanium Pseudomonas, Mucor, Neurospora, Pediococcus, Penicillium notatum Category, Pichia pastoris Pseudomonas, Rhizopus, rhodosporidium toruloides Pseudomonas, rhodotorula Pseudomonas, Blastocystis, Schizosccharomyces, hair spore Sub- Blastocystis has spore Torula, Torulopsis, Verticillium dahliae category, Ye Luoweiya Blastocystis, Zygosaccharomyces Pseudomonas Or Zygotorulaspora.
9. method or purposes according to embodiment 6, wherein the yeast supernatants of the fermentation are made wine ferment by yeast Mother generates.
10. method or purposes according to embodiment 6, wherein the bacterial supernatant of the fermentation is by belonging to following The bacterial species of category generate：It is acetobacter, Arthrobacter, Aerococcus, Bacillus, Bifidobacterium, short shape Bacillus, short Bacillus, Barnobacterium, meat Bacillus, rodlike Pseudomonas, enterococcus spp, Colibacter, glucose acetobacter, Portugal Saccharobacillus category, Hafnia (Hafnia), Halomonas (Halomonas), Cook Pseudomonas, Lactobacillus, galactococcus Category, Leuconostoc, megacoccus category, Microbacterium, Micrococcus, neisseria, wine Coccus, Pediococcus, Propionibacterium Category, Proteus, Pseudomonas, Psychrobacter, Salmonella, lactobacillus, staphylococcus, streptococcus, Streptomyces, Tetracoccus (Tetragenococcus), Vagococcus (Vagococcus), Wei Si Bordetella or fermentation Zygosaccharomyces.
11. method or purposes according to embodiment 6, wherein the bacterial supernatant of the fermentation is by belonging to Aspergillus The mould species of category generate.
12. the method according to embodiment 1-3 or 7-11 or the purposes according to embodiment 4-11, wherein institute State the microorganism supernatant that plant agent composition includes the by weight at least 35% processed fermentation.
13. the method according to embodiment 1-3 or 7-12 or the purposes according to embodiment 4-12, wherein institute State the microorganism supernatant that plant agent composition includes the by weight at most 95% processed fermentation.
14. the method according to embodiment 1-3 or 7-13 or the purposes according to embodiment 4-13, wherein institute State nonionic surface active agent include it is following, be substantially made up of or be made up of：Polyethers non-ionic surface is lived Property agent, polyhydroxy nonionic surface active agent and/or non-ionic biosurfactant.
15. method or purposes according to embodiment 14, wherein the polyhydroxy nonionic surface active agent packet Include it is following, be substantially made up of or be made up of：Sucrose ester, the sucrose ester of ethoxylation, sorbitol ester, ethyoxyl Sorbitol ester, alkyl-glucoside, the alkyl-glucoside of ethoxylation, polyglycerol ester or the polyglycereol of ethoxylation of change Ester.
16. the method according to embodiment 1-3 or 7-15 or the purposes according to embodiment 4-15, wherein institute State nonionic surface active agent include it is following, be substantially made up of or be made up of：Amine oxide, ethoxylation Alcohol, the aliphatic alcohol of ethoxylation, alkylamine, the alkylamine of ethoxylation, the alkylphenol of ethoxylation, alkyl polysaccharide, second The alkyl polysaccharide of oxygroup, the aliphatic acid of ethoxylation, the fatty alcohol of ethoxylation or the fatty amine of ethoxylation have H (OCH2CH2)xOC6H4R1、(OCH2CH2)xOR2Or H (OCH2CH2)xOC(O)R2General formula nonionic surface active agent, wherein X expressions are added into the molal quantity of the ethylene oxide of alkylphenol and/or fatty alcohol or aliphatic acid, R1Represent long chain alkyl group And R2Represent long aliphatic radicals.
17. method or purposes according to embodiment 16, wherein R1It is C7-C10Alkyl group (the normal- of positive structure Alkyl group) and/or wherein R2It is C12-C20Aliphatic group.
18. the method according to embodiment 1-3 or 7-17 or the purposes according to embodiment 4-17, wherein institute State nonionic surface active agent be the nonyl phenol of ethoxylation, the octyl phenol of ethoxylation, ethoxylation cetyl Oleyl alcohol, the cetyl stearyl alcohol of ethoxylation, the decyl alcohol of ethoxylation, the lauryl alcohol of ethoxylation, ethoxylation tridecanol, Or the castor oil of ethoxylation.
19. the method according to embodiment 1-3 or 7-18 or the purposes according to embodiment 4-18, wherein institute State one or more of non-ionic surfactants that plant agent composition includes by weight from about 1% to about 15% Agent.
20. according to the method described in embodiment 19, wherein the plant agent composition include by weight from about 5% to About 13% one or more of nonionic surface active agent.
21. according to the method described in embodiment 20, wherein the plant agent composition include by weight from about 7% to About 11% one or more of nonionic surface active agent.
22. the method according to embodiment 1-3 or 7-21 or the purposes according to embodiment 4-21, wherein institute State plant agent composition also comprising it is following, be substantially made up of or be made up of：One or more of anionic tables Face activating agent.
23. method or purposes according to embodiment 22, wherein the plant agent composition include by weight from About 0.5% to about 10% one or more of anionic surfactant.
24. method or purposes according to embodiment 23, wherein the plant agent composition include by weight from About 1% to about 8% one or more of anionic surfactant.
25. method or purposes according to embodiment 24, wherein the plant agent composition include by weight from About 2% to about 6% one or more of anionic surfactant.
26. the method according to embodiment 1-3 or 7-25 or the purposes according to embodiment 4-25, wherein institute It is at most 4.5 to state pH.
27. method or purposes according to embodiment 26, wherein the pH is about 3.7 to about 4.2.
28. the method according to embodiment 1-3 or 7-27 or the purposes according to embodiment 4-27, wherein institute It is generally nontoxic to the mankind, mammal, plant and environment to state plant agent composition.
29. the method according to embodiment 1-3 or 7-28 or the purposes according to embodiment 4-28, wherein institute It is biodegradable to state plant agent composition.
30. the method according to embodiment 1 or 7-29 or the purposes according to embodiment 4 or 7-29, wherein The effective quantity of the plant agent composition leads to the adverse effect to seeking the controlled virulence factor.
31. method or purposes according to embodiment 30, wherein the effective quantity of the plant agent composition is unfavorable Seek the controlled virulence factor below ground influence：About 70%, about 75%, about 80%, about 85%, about 86%, about 87%th, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98% or about 99%；Or at least 70%, at least 75%, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%th, at least 89%, at least about 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%th, at least 97%, at least 98% or at least 99%；Or at most 70%, at most 75%, at most 80%, at most 85%, at most 86%th, at most 87%, at most 88%, at most 89%, at least more 90%, at most 91%, at most 92%, at most 93%, at most 94%th, at most 95%, at most 96%, at most 97%, at most 98% or at most 99%；Or about 70% to about 80%, about 70% to About 85%, about 70% to about 90%, about 70% to about 95%, about 70% to about 99%, about 75% to about 85%, about 75% to about 90%th, about 75% to about 95%, about 75% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 99%th, about 85% to about 93%, about 85% to about 95%, about 85% to about 97%, about 85% to about 99%, about 90% to about 93%th, about 90% to about 95%, about 90% to about 97%, about 90% to about 99%, about 93% to about 95%, about 93% to about 97%th, about 93% to about 99%, about 95% to about 97% or about 95% to about 99%.
32. the method according to embodiment 1 or 7-31 or the purposes according to embodiment 4 or 7-31, wherein The effective quantity of the plant agent composition causes to seek the death rate of the controlled virulence factor.
33. method or purposes according to embodiment 32, wherein the effective quantity of the plant agent composition causes The following death rate for seeking the controlled virulence factor：About 70%, about 75%, about 80%, about 85%, about 86%, about 87%th, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98% or about 99%；Or at least 70%, at least 75%, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%th, at least 89%, at least about 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%th, at least 97%, at least 98% or at least 99%；Or at most 70%, at most 75%, at most 80%, at most 85%, at most 86%th, at most 87%, at most 88%, at most 89%, at least more 90%, at most 91%, at most 92%, at most 93%, at most 94%th, at most 95%, at most 96%, at most 97%, at most 98% or at most 99%；Or about 70% to about 80%, about 70% to About 85%, about 70% to about 90%, about 70% to about 95%, about 70% to about 99%, about 75% to about 85%, about 75% to about 90%th, about 75% to about 95%, about 75% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 99%th, about 85% to about 93%, about 85% to about 95%, about 85% to about 97%, about 85% to about 99%, about 90% to about 93%th, about 90% to about 95%, about 90% to about 97%, about 90% to about 99%, about 93% to about 95%, about 93% to about 97%th, about 93% to about 99%, about 95% to about 97% or about 95% to about 99%.
34. the method according to embodiment 1 or 7-33 or the purposes according to embodiment 4 or 7-33, wherein The size of the group of the controlled virulence factor is sought in the effective quantity reduction of the plant agent composition.
35. method or purposes according to embodiment 34, wherein the effective quantity of the plant agent composition reduces Seek the size of the group of controlled virulence factor：About 70%, about 75%, about 80%, about 85%, about 86%, about 87%, about 88%th, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98% or About 99%；Or at least 70%, at least 75%, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%th, at least about 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%th, at least 98% or at least 99%；Or at most 70%, at most 75%, at most 80%, at most 85%, at most 86%, at most 87%th, at most 88%, at most 89%, at least more 90%, at most 91%, at most 92%, at most 93%, at most 94%, at most 95%th, at most 96%, at most 97%, at most 98% or at most 99%；Or about 70% to about 80%, about 70% to about 85%, about 70% to about 90%, about 70% to about 95%, about 70% to about 99%, about 75% to about 85%, about 75% to about 90%, about 75% to about 95%, about 75% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 99%, about 85% to about 93%, about 85% to about 95%, about 85% to about 97%, about 85% to about 99%, about 90% to about 93%, about 90% to about 95%, about 90% to about 97%, about 90% to about 99%, about 93% to about 95%, about 93% to about 97%, about 93% to about 99%, about 95% to about 97% or about 95% to about 99%.
36. the method according to embodiment 1-3 or 7-35 or the purposes according to embodiment 4 or 7-35, Described in the effective quantity prevention of plant agent composition seek the group of controlled virulence factor and enter or infect one or more Multiple places.
37. method or purposes according to embodiment 36, wherein the effective quantity of the plant agent composition prevents The group of the following virulence factor enters or infects one or more places：About 70%, about 75%, about 80%, about 85%th, about 86%, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%th, about 97%, about 98% or about 99%；Or at least 70%, at least 75%, at least 80%, at least 85%, at least 86%, extremely Few 87%, at least 88%, at least 89%, at least about 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%th, at least 96%, at least 97%, at least 98% or at least 99%；Or at most 70%, at most 75%, at most 80%, at most 85%th, at most 86%, at most 87%, at most 88%, at most 89%, at least more 90%, at most 91%, at most 92%, at most 93%th, at most 94%, at most 95%, at most 96%, at most 97%, at most 98% or at most 99%；Or about 70% to about 80%, About 70% to about 85%, about 70% to about 90%, about 70% to about 95%, about 70% to about 99%, about 75% to about 85%, about 75% to about 90%, about 75% to about 95%, about 75% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 99%, about 85% to about 93%, about 85% to about 95%, about 85% to about 97%, about 85% to about 99%, about 90% to about 93%, about 90% to about 95%, about 90% to about 97%, about 90% to about 99%, about 93% to about 95%, about 93% to about 97%, about 93% to about 99%, about 95% to about 97% or about 95% to about 99%.
38. the method according to embodiment 2 or 7-29 or the purposes according to embodiment 5 or 7-29, wherein The effective quantity of the plant agent composition improves the suction by the water of root hair, minerals and other nutrients from soil It receives.
39. method or purposes according to embodiment 38, wherein the effective quantity of the plant agent composition improves Pass through the absorption of the water of root hair, minerals and other nutrients from soil：About 70%, about 75%, about 80%, about 85%th, about 86%, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%th, about 97%, about 98% or about 99%；Or at least 70%, at least 75%, at least 80%, at least 85%, at least 86%, extremely Few 87%, at least 88%, at least 89%, at least about 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%th, at least 96%, at least 97%, at least 98% or at least 99%；Or at most 70%, at most 75%, at most 80%, at most 85%th, at most 86%, at most 87%, at most 88%, at most 89%, at least more 90%, at most 91%, at most 92%, at most 93%th, at most 94%, at most 95%, at most 96%, at most 97%, at most 98% or at most 99%；Or about 70% to about 80%, About 70% to about 85%, about 70% to about 90%, about 70% to about 95%, about 70% to about 99%, about 75% to about 85%, about 75% to about 90%, about 75% to about 95%, about 75% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 99%, about 85% to about 93%, about 85% to about 95%, about 85% to about 97%, about 85% to about 99%, about 90% to about 93%, about 90% to about 95%, about 90% to about 97%, about 90% to about 99%, about 93% to about 95%, about 93% to about 97%, about 93% to about 99%, about 95% to about 97% or about 95% to about 99%.
40. method according to embodiment 2,7-29 or 38-39 or according to embodiment 5,7-29 or 38-39 Purposes, flowed wherein the effective quantity of the plant agent composition is improved by the xylem sap of xylem.
41. method or purposes according to embodiment 40, wherein the effective quantity of the plant agent composition improves It is flowed by the xylem sap of xylem：About 70%, about 75%, about 80%, about 85%, about 86%, about 87%, about 88%, About 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98% or about 99%；Or at least 70%, at least 75%, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%th, at least about 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%th, at least 98% or at least 99%；Or at most 70%, at most 75%, at most 80%, at most 85%, at most 86%, at most 87%th, at most 88%, at most 89%, at least more 90%, at most 91%, at most 92%, at most 93%, at most 94%, at most 95%th, at most 96%, at most 97%, at most 98% or at most 99%；Or about 70% to about 80%, about 70% to about 85%, about 70% to about 90%, about 70% to about 95%, about 70% to about 99%, about 75% to about 85%, about 75% to about 90%, about 75% to about 95%, about 75% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 99%, about 85% to about 93%, about 85% to about 95%, about 85% to about 97%, about 85% to about 99%, about 90% to about 93%, about 90% to about 95%, about 90% to about 97%, about 90% to about 99%, about 93% to about 95%, about 93% to about 97%, about 93% to about 99%, about 95% to about 97% or about 95% to about 99%.
42. method according to embodiment 2,7-29 or 38-41 or according to embodiment 5,7-29 or 38-41 Purposes, wherein the plant agent composition the effective quantity improve bast in photosynthate flowing.
43. method or purposes according to embodiment 42, wherein the effective quantity of the plant agent composition improves Photosynthate flowing in bast：About 70%, about 75%, about 80%, about 85%, about 86%, about 87%, about 88%, About 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98% or about 99%；Or at least 70%, at least 75%, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%th, at least about 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%th, at least 98% or at least 99%；Or at most 70%, at most 75%, at most 80%, at most 85%, at most 86%, at most 87%th, at most 88%, at most 89%, at least more 90%, at most 91%, at most 92%, at most 93%, at most 94%, at most 95%th, at most 96%, at most 97%, at most 98% or at most 99%；Or about 70% to about 80%, about 70% to about 85%, about 70% to about 90%, about 70% to about 95%, about 70% to about 99%, about 75% to about 85%, about 75% to about 90%, about 75% to about 95%, about 75% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 99%, about 85% to about 93%, about 85% to about 95%, about 85% to about 97%, about 85% to about 99%, about 90% to about 93%, about 90% to about 95%, about 90% to about 97%, about 90% to about 99%, about 93% to about 95%, about 93% to about 97%, about 93% to about 99%, about 95% to about 97% or about 95% to about 99%.
44. method according to embodiment 2,7-29 or 38-43 or according to embodiment 5,7-29 or 38-43 Purposes, wherein the effective quantity of the plant agent composition increases water, minerals and other nutrients from soil It absorbs.
45. method or purposes according to embodiment 44, wherein the effective quantity of the plant agent composition increases The absorption of water, minerals and other nutrients from soil：About 70%, about 75%, about 80%, about 85%, about 86%, about 87%th, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98% or about 99%；Or at least 70%, at least 75%, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%th, at least 89%, at least about 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%th, at least 97%, at least 98% or at least 99%；Or at most 70%, at most 75%, at most 80%, at most 85%, at most 86%th, at most 87%, at most 88%, at most 89%, at least more 90%, at most 91%, at most 92%, at most 93%, at most 94%th, at most 95%, at most 96%, at most 97%, at most 98% or at most 99%；Or about 70% to about 80%, about 70% to About 85%, about 70% to about 90%, about 70% to about 95%, about 70% to about 99%, about 75% to about 85%, about 75% to about 90%th, about 75% to about 95%, about 75% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 99%th, about 85% to about 93%, about 85% to about 95%, about 85% to about 97%, about 85% to about 99%, about 90% to about 93%th, about 90% to about 95%, about 90% to about 97%, about 90% to about 99%, about 93% to about 95%, about 93% to about 97%th, about 93% to about 99%, about 95% to about 97% or about 95% to about 99%.
46. method according to embodiment 2,7-29 or 38-45 or according to embodiment 5,7-29 or 38-45 Purposes, wherein the plant agent composition the effective quantity increase xylem in capillarity and/or hydrostatic pressing.
47. method or purposes according to embodiment 46, wherein the effective quantity of the plant agent composition increases Capillarity and/or hydrostatic pressing in xylem：About 70%, about 75%, about 80%, about 85%, about 86%, about 87%, about 88%th, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98% or About 99%；Or at least 70%, at least 75%, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%th, at least about 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%th, at least 98% or at least 99%；Or at most 70%, at most 75%, at most 80%, at most 85%, at most 86%, at most 87%th, at most 88%, at most 89%, at least more 90%, at most 91%, at most 92%, at most 93%, at most 94%, at most 95%th, at most 96%, at most 97%, at most 98% or at most 99%；Or about 70% to about 80%, about 70% to about 85%, about 70% to about 90%, about 70% to about 95%, about 70% to about 99%, about 75% to about 85%, about 75% to about 90%, about 75% to about 95%, about 75% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 99%, about 85% to about 93%, about 85% to about 95%, about 85% to about 97%, about 85% to about 99%, about 90% to about 93%, about 90% to about 95%, about 90% to about 97%, about 90% to about 99%, about 93% to about 95%, about 93% to about 97%, about 93% to about 99%, about 95% to about 97% or about 95% to about 99%.
48. method according to embodiment 2,7-29 or 38-47 or according to embodiment 5,7-29 or 38-47 Purposes, wherein the plant agent composition the effective quantity improve transport of the raw material by the plant.
49. method or purposes according to embodiment 48, wherein the effective quantity of the plant agent composition improves The transport that raw material pass through the plant：About 70%, about 75%, about 80%, about 85%, about 86%, about 87%, about 88%, about 89%th, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98% or about 99%； Or at least 70%, at least 75%, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, extremely Few about 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99%；Or at most 70%, at most 75%, at most 80%, at most 85%, at most 86%, at most 87%, at most 88%th, at most 89%, at least more 90%, at most 91%, at most 92%, at most 93%, at most 94%, at most 95%, at most 96%th, at most 97%, at most 98% or at most 99%；Or about 70% to about 80%, about 70% to about 85%, about 70% to about 90%th, about 70% to about 95%, about 70% to about 99%, about 75% to about 85%, about 75% to about 90%, about 75% to about 95%th, about 75% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 99%, about 85% to about 93%th, about 85% to about 95%, about 85% to about 97%, about 85% to about 99%, about 90% to about 93%, about 90% to about 95%th, about 90% to about 97%, about 90% to about 99%, about 93% to about 95%, about 93% to about 97%, about 93% to about 99%th, about 95% to about 97% or about 95% to about 99%.
50. method according to embodiment 2,7-29 or 38-49 or according to embodiment 5,7-29 or 38-49 Purposes, wherein the effective quantity of the plant agent composition increases the synthesis of compound and energy in the plant.
51. method or purposes according to embodiment 50, wherein the effective quantity of the plant agent composition increases The synthesis of compound and energy in the plant：About 70%, about 75%, about 80%, about 85%, about 86%, about 87%, about 88%th, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98% or About 99%；Or at least 70%, at least 75%, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%th, at least about 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%th, at least 98% or at least 99%；Or at most 70%, at most 75%, at most 80%, at most 85%, at most 86%, at most 87%th, at most 88%, at most 89%, at least more 90%, at most 91%, at most 92%, at most 93%, at most 94%, at most 95%th, at most 96%, at most 97%, at most 98% or at most 99%；Or about 70% to about 80%, about 70% to about 85%, about 70% to about 90%, about 70% to about 95%, about 70% to about 99%, about 75% to about 85%, about 75% to about 90%, about 75% to about 95%, about 75% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 99%, about 85% to about 93%, about 85% to about 95%, about 85% to about 97%, about 85% to about 99%, about 90% to about 93%, about 90% to about 95%, about 90% to about 97%, about 90% to about 99%, about 93% to about 95%, about 93% to about 97%, about 93% to about 99%, about 95% to about 97% or about 95% to about 99%.
52. method according to embodiment 2,7-29 or 38-51 or according to embodiment 5,7-29 or 38-51 Purposes, wherein the plant agent composition the effective quantity improve maintain and continue plant growth needed for compound and energy Synthesis.
53. method or purposes according to embodiment 52, wherein the effective quantity of the plant agent composition improves Maintain and continue the synthesis of the compound and energy needed for plant growth：About 70%, about 75%, about 80%, about 85%, about 86%th, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%th, about 98% or about 99%；Or at least 70%, at least 75%, at least 80%, at least 85%, at least 86%, at least 87%, At least 88%, at least 89%, at least about 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, extremely Few 96%, at least 97%, at least 98% or at least 99%；Or at most 70%, at most 75%, at most 80%, at most 85%, at most 86%th, at most 87%, at most 88%, at most 89%, at least more 90%, at most 91%, at most 92%, at most 93%, at most 94%th, at most 95%, at most 96%, at most 97%, at most 98% or at most 99%；Or about 70% to about 80%, about 70% to About 85%, about 70% to about 90%, about 70% to about 95%, about 70% to about 99%, about 75% to about 85%, about 75% to about 90%th, about 75% to about 95%, about 75% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 99%th, about 85% to about 93%, about 85% to about 95%, about 85% to about 97%, about 85% to about 99%, about 90% to about 93%th, about 90% to about 95%, about 90% to about 97%, about 90% to about 99%, about 93% to about 95%, about 93% to about 97%th, about 93% to about 99%, about 95% to about 97% or about 95% to about 99%.
54. method according to embodiment 2,7-29 or 38-53 or according to embodiment 5,7-29 or 38-53 Purposes, wherein the plant agent composition the effective quantity dissolving, dispersion or otherwise remove destroy xylem in wood One or more of components of matter portion juice flowing.
55. method or purposes according to embodiment 54, wherein the effective quantity dissolving of the plant agent composition, Dispersion otherwise removes the one or more of components for destroying that xylem sap flows in xylem：About 70%, about 75%th, about 80%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%th, about 95%, about 96%, about 97%, about 98% or about 99%；Or at least 70%, at least 75%, at least 80%, at least 85%th, at least 86%, at least 87%, at least 88%, at least 89%, at least about 90%, at least 91%, at least 92%, at least 93%th, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99%；Or at most 70%, at most 75%th, at most 80%, at most 85%, at most 86%, at most 87%, at most 88%, at most 89%, at least more 90%, at most 91%th, at most 92%, at most 93%, at most 94%, at most 95%, at most 96%, at most 97%, at most 98% or at most 99%；Or about 70% to about 80%, about 70% to about 85%, about 70% to about 90%, about 70% to about 95%, about 70% to about 99%th, about 75% to about 85%, about 75% to about 90%, about 75% to about 95%, about 75% to about 99%, about 80% to about 90%th, about 80% to about 95%, about 80% to about 99%, about 85% to about 93%, about 85% to about 95%, about 85% to about 97%th, about 85% to about 99%, about 90% to about 93%, about 90% to about 95%, about 90% to about 97%, about 90% to about 99%th, about 93% to about 95%, about 93% to about 97%, about 93% to about 99%, about 95% to about 97% or about 95% to about 99%.
56. method according to embodiment 2,7-29 or 38-55 or according to embodiment 5,7-29 or 38-55 Purposes, wherein the plant agent composition the effective quantity dissolving, dispersion or otherwise remove destroy bast in light One or more of components that cooperation product flows.
57. method or purposes according to embodiment 56, wherein the effective quantity dissolving of the plant agent composition, Dispersion otherwise removes the one or more of components for destroying that photosynthate flows in bast：About 70%, about 75%th, about 80%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%th, about 95%, about 96%, about 97%, about 98% or about 99%；Or at least 70%, at least 75%, at least 80%, at least 85%th, at least 86%, at least 87%, at least 88%, at least 89%, at least about 90%, at least 91%, at least 92%, at least 93%th, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99%；Or at most 70%, at most 75%th, at most 80%, at most 85%, at most 86%, at most 87%, at most 88%, at most 89%, at least more 90%, at most 91%th, at most 92%, at most 93%, at most 94%, at most 95%, at most 96%, at most 97%, at most 98% or at most 99%；Or about 70% to about 80%, about 70% to about 85%, about 70% to about 90%, about 70% to about 95%, about 70% to about 99%th, about 75% to about 85%, about 75% to about 90%, about 75% to about 95%, about 75% to about 99%, about 80% to about 90%th, about 80% to about 95%, about 80% to about 99%, about 85% to about 93%, about 85% to about 95%, about 85% to about 97%th, about 85% to about 99%, about 90% to about 93%, about 90% to about 95%, about 90% to about 97%, about 90% to about 99%th, about 93% to about 95%, about 93% to about 97%, about 93% to about 99%, about 95% to about 97% or about 95% to about 99%.
58. the method according to embodiment 3 or 7-29 or the purposes according to embodiment 6-29, wherein described The effective quantity dissolving of plant agent composition disperses or otherwise removes water flowing in the pipeline network for destroying irrigation system One or more of components.
59. method or purposes according to embodiment 58, wherein the effective quantity dissolving of the plant agent composition, Dispersion or the one or more of components for otherwise removing water flowing in the pipeline network for destroying irrigation system：About 70%, About 75%, about 80%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, About 94%, about 95%, about 96%, about 97%, about 98% or about 99%；Or at least 70%, at least 75%, at least 80%, at least 85%th, at least 86%, at least 87%, at least 88%, at least 89%, at least about 90%, at least 91%, at least 92%, at least 93%th, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99%；Or at most 70%, at most 75%th, at most 80%, at most 85%, at most 86%, at most 87%, at most 88%, at most 89%, at least more 90%, at most 91%th, at most 92%, at most 93%, at most 94%, at most 95%, at most 96%, at most 97%, at most 98% or at most 99%；Or about 70% to about 80%, about 70% to about 85%, about 70% to about 90%, about 70% to about 95%, about 70% to about 99%th, about 75% to about 85%, about 75% to about 90%, about 75% to about 95%, about 75% to about 99%, about 80% to about 90%th, about 80% to about 95%, about 80% to about 99%, about 85% to about 93%, about 85% to about 95%, about 85% to about 97%th, about 85% to about 99%, about 90% to about 93%, about 90% to about 95%, about 90% to about 97%, about 90% to about 99%th, about 93% to about 95%, about 93% to about 97%, about 93% to about 99%, about 95% to about 97% or about 95% to about 99%.
60. method according to embodiment 3,7-29 or 58-59 or according to embodiment 6-29 or 58-59 Purposes, wherein the effective quantity of the plant agent composition improves the water transport in the pipeline network of entire irrigation system.
61. method or purposes according to embodiment 60, wherein the effective quantity of the plant agent composition improves Water transport in the pipeline network of entire irrigation system：About 70%, about 75%, about 80%, about 85%, about 86%, about 87%th, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98% or about 99%；Or at least 70%, at least 75%, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%th, at least 89%, at least about 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%th, at least 97%, at least 98% or at least 99%；Or at most 70%, at most 75%, at most 80%, at most 85%, at most 86%th, at most 87%, at most 88%, at most 89%, at least more 90%, at most 91%, at most 92%, at most 93%, at most 94%th, at most 95%, at most 96%, at most 97%, at most 98% or at most 99%；Or about 70% to about 80%, about 70% to About 85%, about 70% to about 90%, about 70% to about 95%, about 70% to about 99%, about 75% to about 85%, about 75% to about 90%th, about 75% to about 95%, about 75% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 99%th, about 85% to about 93%, about 85% to about 95%, about 85% to about 97%, about 85% to about 99%, about 90% to about 93%th, about 90% to about 95%, about 90% to about 97%, about 90% to about 99%, about 93% to about 95%, about 93% to about 97%th, about 93% to about 99%, about 95% to about 97% or about 95% to about 99%.
62. the method according to embodiment 1 or 7-61 or the purposes according to embodiment 4-61, wherein described The effective quantity of plant agent composition is kept and/or the health and vigor of enhancing plant.
63. method or purposes according to embodiment 62, wherein the effective quantity of the plant agent composition is kept And/or the health and vigor of enhancing plant：About 70%, about 75%, about 80%, about 85%, about 86%, about 87%, about 88%, about 89%th, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98% or about 99%； Or at least 70%, at least 75%, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, extremely Few about 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99%；Or at most 70%, at most 75%, at most 80%, at most 85%, at most 86%, at most 87%, at most 88%th, at most 89%, at least more 90%, at most 91%, at most 92%, at most 93%, at most 94%, at most 95%, at most 96%th, at most 97%, at most 98% or at most 99%；Or about 70% to about 80%, about 70% to about 85%, about 70% to about 90%th, about 70% to about 95%, about 70% to about 99%, about 75% to about 85%, about 75% to about 90%, about 75% to about 95%th, about 75% to about 99%, about 80% to about 90%, about 80% to about 95%, about 80% to about 99%, about 85% to about 93%th, about 85% to about 95%, about 85% to about 97%, about 85% to about 99%, about 90% to about 93%, about 90% to about 95%th, about 90% to about 97%, about 90% to about 99%, about 93% to about 95%, about 93% to about 97%, about 93% to about 99%th, about 95% to about 97% or about 95% to about 99%.
64. the method according to embodiment 1-3 or 7-63 or the purposes according to embodiment 4-63, wherein institute The effective quantity for stating plant agent composition is following plant agent composition:Diluent ratio：About 1:50th, about 1:75th, about 1: 100th, about 1:125th, about 1:150th, about 1:175th, about 1:200th, about 1:225th, about 1:250th, about 1:275th, about 1:300th, about 1:325、 About 1:350th, about 1:375th, about 1:400th, about 1:425th, about 1:450th, about 1:475th, about 1:500th, about 1:525th, about 1:550th, about 1: 575 or about 1:600；Or at least 1:50th, at least 1:75th, at least 1:100th, at least 1:125th, at least 1:150th, at least 1:175th, at least 1:200th, at least 1:225th, at least 1:250th, at least 1:275th, at least 1:300th, at least 1:325th, at least 1:350th, at least 1:375、 At least 1:400th, at least 1:425th, at least 1:450th, at least 1:475th, at least 1:500th, at least 1:525th, at least 1:550th, at least 1: 575 or at least 1:600；Or at most 1:50th, at most 1:75th, at most 1:100th, at most 1:125th, at most 1:150th, at most 1:175th, extremely More 1:200th, at most 1:225th, at most 1:250th, at most 1:275th, at most 1:300th, at most 1:325th, at most 1:350th, at most 1: 375th, at most 1:400th, at most 1:425th, at most 1:450th, at most 1:475th, at most 1:500th, at most 1:525th, at most 1:550th, extremely More 1:575 or at most 1:600；Or about 1:50 to about 1:100th, about 1:50 to about 1:200th, about 1:50 to about 1:300th, about 1:50 to About 1:400th, about 1:50 to about 1:500th, about 1:50 to about 1:600th, about 1:100 to about 1:200th, about 1:100 to about 1:300th, about 1:100 to about 1:400th, about 1:100 to about 1:500th, about 1:100 to about 1:600th, about 1:200 to about 1:300th, about 1:200 to about 1:400th, about 1:200 to about 1:500th, about 1:200 to about 1:600th, about 1:300 to about 1:400th, about 1:300 to about 1:500th, about 1:300 to about 1:600th, about 1:400 to about 1:500th, about 1:400 to about 1:600 or about 1:500 to about 1:600.
65. the method according to embodiment 1-3 or 7-63 or the purposes according to embodiment 4-63, wherein institute The effective quantity for stating plant agent composition is following plant agent composition:Diluent ratio：About 1:500th, about 1:750th, about 1: 1000th, about 1:1250th, about 1:1500th, about 1:1750th, about 1:2000th, about 1:2250th, about 1:2500th, about 1:2750th, about 1:3000、 About 1:3250th, about 1:3500th, about 1:3750th, about 1:4000th, about 1:4250th, about 1:4500th, about 1:4750th, about 1:5000th, about 1: 5250th, about 1:5500th, about 1:5750th, about 1:6000th, about 1:7000th, about 1:8000th, about 1:9000 or about 1:10000；Or at least 1:500th, at least 1:750th, at least 1:1000th, at least 1:1250th, at least 1:1500th, at least 1:1750th, at least 1:2000th, at least 1: 2250th, at least 1:2500th, at least 1:2750th, at least 1:3000th, at least 1:3250th, at least 1:3500th, at least 1:3750th, at least 1: 4000th, at least 1:4250th, at least 1:4500th, at least 1:4750th, at least 1:5000th, at least 1:5250th, at least 1:5500th, at least 1: 5750th, at least 1:6000th, at least 1:7000th, at least 1:8000th, at least 1:9000 or at least 1:10000；Or at most 1:500th, extremely More 1:750th, at most 1:1000th, at most 1:1250th, at most 1:1500th, at most 1:1750th, at most 1:2000th, at most 1:2250th, extremely More 1:2500th, at most 1:2750th, at most 1:3000th, at most 1:3250th, at most 1:3500th, at most 1:3750th, at most 1:4000th, extremely More 1:4250th, at most 1:4500th, at most 1:4750th, at most 1:5000th, at most 1:5250th, at most 1:5500th, at most 1:5750th, extremely More 1:6000th, at most 1:7000th, at most 1:8000th, at most 1:9000 or at most 1:10000；Or about 1:500 to about 1:1000th, about 1:500 to about 1:2000th, about 1:500 to about 1:3000th, about 1:500 to about 1:4000th, about 1:500 to about 1:5000th, about 1:500 To about 1:6000th, about 1:500 to about 1:7000th, about 1:500 to about 1:8000th, about 1:500 to about 1:9000th, about 1:500 to about 1:10000th, about 1:1000 to about 1:2000th, about 1:1000 to about 1:3000th, about 1:1000 to about 1:4000th, about 1:1000 to about 1:5000th, about 1:1000 to about 1:6000th, about 1:1000 to about 1:7000th, about 1:1000 to about 1:8000th, about 1:1000 to about 1:9000th, about 1:1000 to about 1:10000th, about 1:2000 to about 1:3000th, about 1:2000 to about 1:4000th, about 1:2000 to about 1:5000th, about 1:2000 to about 1:6000th, about 1:2000 to about 1:7000th, about 1:2000 to about 1:8000th, about 1:2000 to about 1:9000th, about 1:2000 to about 1:10000th, about 1:3000 to about 1:4000th, about 1:3000 to about 1:5000th, about 1:3000 to about 1:6000th, about 1:3000 to about 1:7000th, about 1:3000 to about 1:8000th, about 1:3000 to about 1:9000th, about 1:3000 to about 1:10000th, about 1:4000 to about 1:5000th, about 1:4000 to about 1:6000th, about 1:4000 to about 1:7000th, about 1:4000 to about 1:8000th, about 1:4000 to about 1:9000th, about 1:4000 to about 1:10000th, about 1:5000 to about 1:6000th, about 1:5000 to about 1:7000th, about 1:5000 to about 1:8000th, about 1:5000 to about 1:9000th, about 1:5000 to about 1:10000th, about 1:6000 to about 1:7000th, about 1:6000 to about 1:8000th, about 1:6000 to about 1:9000th, about 1:6000 to about 1:10000th, about 1:7000 to about 1:8000th, about 1:7000 to about 1:9000th, about 1:7000 to about 1:10000th, about 1:8000 to about 1:9000th, about 1:8000 to about 1:10000 or about 1:9000 to about 1:10000.
66. the method according to embodiment 1-3 or 7-65 or the purposes according to embodiment 4-65, wherein institute The effective quantity for stating plant agent composition has following ultimate density：About 0.0001%, about 0.0002%, about 0.0003%, About 0.0004%, about 0.0005%, about 0.0006%, about 0.0007%, about 0.0008%, about 0.0009%, about 0.001%, About 0.002%, about 0.003%, about 0.004%, about 0.005%, about 0.006%, about 0.007%, about 0.008%, about 0.009%th, about 0.01%, about 0.02%, about 0.03%, about 0.04%, about 0.05%, about 0.06%, about 0.07%, about 0.08%th, about 0.09%, about 0.1%, about 0.2%, about 0.3%, about 0.4%, about 0.5%, about 0.6%, about 0.7%, about 0.8%th, about 0.9%, about 1%, about 2%, about 3%, about 4%, about 5%, about 6%, about 7%, about 8%, about 9% or about 10%； Or at least 0.0001%, at least 0.0002%, at least 0.0003%, at least 0.0004%, at least 0.0005%, at least 0.0006%th, at least 0.0007%, at least 0.0008%, at least 0.0009%, at least 0.001%, at least 0.002%, at least 0.003%th, at least 0.004%, at least 0.005%, at least 0.006%, at least 0.007%, at least 0.008%, at least 0.009%th, at least 0.01%, at least 0.02%, at least 0.03%, at least 0.04%, at least 0.05%, at least 0.06%, extremely Few 0.07%, at least 0.08%, at least 0.09%, at least 0.1%, at least 0.2%, at least 0.3%, at least 0.4%, at least 0.5%th, at least 0.6%, at least 0.7%, at least 0.8%, at least 0.9%, at least 1%, at least 2%, at least 3%, at least 4%th, at least 5%, at least 6%, at least 7%, at least 8%, at least 9% or at least 10%；Or at most 0.0001%, at most 0.0002%th, at most 0.0003%, at most 0.0004%, at most 0.0005%, at most 0.0006%, at most 0.0007%, extremely More 0.0008%, at most 0.0009%, at most 0.001%, at most 0.002%, at most 0.003%, at most 0.004%, at most 0.005%th, at most 0.006%, at most 0.007%, at most 0.008%, at most 0.009%, at most 0.01%, at most 0.02%th, at most 0.03%, at most 0.04%, at most 0.05%, at most 0.06%, at most 0.07%, at most 0.08%, at most 0.09%th, at most 0.1%, at most 0.2%, at most 0.3%, at most 0.4%, at most 0.5%, at most 0.6%, at most 0.7%, At most 0.8%, at most 0.9%, at most 1%, at most 2%, at most 3%, at most 4%, at most 5%, at most 6%, at most 7%, At most 8%, at most 9% or at most 10%；Or about 0.0001% to about 0.0005%, about 0.0001% to about 0.001%, about 0.0001% to about 0.005%, about 0.0001% to about 0.01%, about 0.0001% to about 0.05%, about 0.0001% to about 0.1%th, about 0.0001% to about 0.5%, about 0.0001% to about 1%, about 0.0001% to about 5%, about 0.0001% to about 10%th, about 0.0005% to about 0.001%, about 0.0005% to about 0.005%, about 0.0005% to about 0.01%, about 0.0005% to about 0.05%, about 0.0005% to about 0.1%, about 0.0005% to about 0.5%, about 0.0005% to about 1%, About 0.0005% to about 5%, about 0.0005% to about 10%, about 0.001% to about 0.005%, about 0.001% to about 0.01%, 0.001% to about 0.05%, about 0.001% to about 0.1%, 0.001% to about 0.5%, 0.001% to about 1%, 0.001% to about 5%, about 0.001% to about 10%, about 0.005% to about 0.01%, about 0.005% to about 0.05%, about 0.005% to about 0.1%, about 0.005% to about 0.5%, about 0.005% to about 1%, about 0.005% to about 5%, about 0.005% to about 10%, about 0.01% to about 0.05%, about 0.01% to about 0.1%, about 0.01% to about 0.5%, about 0.01% to about 1%, about 0.01% to about 5%, about 0.01% to about 10%, about 0.05% to about 0.1%, about 0.05% to about 0.5%th, about 0.05% to about 1%, about 0.05% to about 5%, about 0.05% to about 10%, about 0.1% to about 0.5%, about 0.1% to about 1%, about 0.1% to about 5%, about 0.1% to about 10%, about 0.5% to about 1%, about 0.5% to about 5%, about 0.5% to about 10%, about 1% to about 5%, about 1% to about 10% or about 5% to about 10%.
67. a kind of plant agent composition, comprising it is following, be substantially made up of or be made up of：Processed hair The microorganism supernatant of ferment and one or more of nonionic surface active agent, wherein the composition does not have any organized enzyme Or bacterium living, and wherein described composition has the pH less than 5.0.
68. the plant agent composition according to embodiment claim 67, wherein the processed fermentation is micro- Yeast supernatants of the biological supernatant from fermentation, the bacterial supernatant to ferment, the mould supernatant to ferment or any combination thereof.
69. the plant agent composition according to embodiment 68, wherein the yeast supernatants of the fermentation by belong to Under category yeast species generate：Brettanomyce Pseudomonas, intends Weir Blastocystis, the black powder yeast of sporangiocyst line at Candida Pseudomonas, Dbaly yeast Pseudomonas, moral carat Blastocystis, Fusarium, geotrichum sp category, her Sa Blastocystis, Kazakhstan Blastocystis, Ke Leke Blastocystis, Kluyveromyces, lecanium Pseudomonas, Mucor, Neurospora, Pediococcus, blueness Mould category, Pichia pastoris Pseudomonas, Rhizopus, rhodosporidium toruloides Pseudomonas, rhodotorula Pseudomonas, Blastocystis, Schizosccharomyces, Hair spore Blastocystis has spore Torula, Torulopsis, Verticillium dahliae category, Ye Luoweiya Blastocystis, Zygosaccharomyces Pseudomonas or Zygotorulaspora.
70. the plant agent composition according to embodiment 69, wherein the yeast supernatants of the fermentation are made by yeast Brewer yeast generates.
71. the plant agent composition according to embodiment 68, wherein the bacterial supernatant of the fermentation by belong to Under category bacterial species generate：Acetobacter, Arthrobacter, Aerococcus, Bacillus, Bifidobacterium, short shape bacillus Category, brevibacterium, Barnobacterium, meat Bacillus, rodlike Pseudomonas, enterococcus spp, Colibacter, glucose acetobacter Category, Gluconobacter, Hafnia (Hafnia), Halomonas (Halomonas), Cook Pseudomonas, Lactobacillus, breast Coccus, Leuconostoc, megacoccus category, Microbacterium, Micrococcus, neisseria, wine Coccus, Pediococcus, propionic acid Bacillus, Proteus, Pseudomonas, Psychrobacter, Salmonella, lactobacillus, staphylococcus, streptococcus Category, streptomyces, Tetracoccus (Tetragenococcus), Vagococcus (Vagococcus), Wei Si Bordetella or hair Zymomonas.
72. the plant agent composition according to embodiment 71, wherein the bacterial supernatant of the fermentation is by belonging to bent The bacterial species that mould belongs to generate.
73. the plant agent composition according to any of embodiment 67-72, wherein the plant agent composition packet Microorganism supernatant containing the by weight at least 35% processed fermentation.
74. the plant agent composition according to any of embodiment 67-73, wherein the plant agent composition packet Microorganism supernatant containing the by weight at most 50% processed fermentation.
75. the plant agent composition according to any of embodiment 67-74, wherein the non-ionic surface is lived Property agent include it is following, be substantially made up of or be made up of：Polyethers nonionic surface active agent, polyhydroxy nonionic Type surfactant and/or biosurfactant.
76. the plant agent composition according to embodiment 75, wherein the polyhydroxy nonionic surface active agent Including it is following, be substantially made up of or be made up of：Sucrose ester, the sucrose ester of ethoxylation, sorbitol ester, ethoxy The sorbitol ester of base, alkyl-glucoside, the alkyl-glucoside of ethoxylation, polyglycerol ester or ethoxylation it is poly- sweet Grease.
77. the plant agent composition according to any of embodiment 67-76, wherein the non-ionic surface is lived Property agent include it is following, be substantially made up of or be made up of：Amine oxide, the alcohol of ethoxylation, ethoxylation fat Fat race alcohol, alkylamine, the alkylamine of ethoxylation, the alkylphenol of ethoxylation, alkyl polysaccharide, the alkyl of ethoxylation are more Sugar, the fatty amine of the aliphatic acid of ethoxylation, the fatty alcohol of ethoxylation or ethoxylation or with H (OCH2CH2)xOC6H4R1、(OCH2CH2)xOR2Or H (OCH2CH2)xOC(O)R2General formula nonionic surface active agent, wherein x represent quilt Added to alkylphenol and/or the molal quantity of the ethylene oxide of fatty alcohol or aliphatic acid, R1Represent long chain alkyl group and R2Table Show long aliphatic radicals.
78. the plant agent composition according to embodiment 77, wherein R1It is C7-C10The alkyl group of positive structure and/or Wherein R2It is C12-C20Aliphatic group.
79. the plant agent composition according to any of embodiment 67-78, wherein the non-ionic surface is lived Property agent be the nonyl phenol of ethoxylation, the octyl phenol of ethoxylation, the cetyl oleyl alcohol of ethoxylation, ethoxylation whale Cerul stearyl alcohol, the decyl alcohol of ethoxylation, the lauryl alcohol of ethoxylation, the tridecanol of ethoxylation or the castor-oil plant of ethoxylation Oil.
80. the plant agent composition according to any of embodiment 67-79, wherein the plant agent composition packet Containing by weight from about 1% to about 15% one or more of nonionic surface active agent.
82. the plant agent composition according to embodiment 80, wherein the plant agent composition includes by weight From about 5% to about 13% one or more of nonionic surface active agent.
83. the plant agent composition according to embodiment 81, wherein the plant agent composition includes by weight From about 7% to about 11% one or more of nonionic surface active agent.
84. the plant agent composition according to any of embodiment 67-83, wherein the plant agent composition is also Comprising it is following, be substantially made up of or be made up of：One or more of anionic surfactant.
85. the plant agent composition according to embodiment 84, wherein the plant agent composition includes by weight From about 0.5% to about 10% one or more of anionic surfactant.
86. the plant agent composition according to embodiment 85, wherein the plant agent composition includes by weight From about 1% to about 8% one or more of anionic surfactant.
87. the plant agent composition according to embodiment 86, wherein the plant agent composition includes by weight From about 2% to about 6% one or more of anionic surfactant.
88. the plant agent composition according to any of embodiment 67-87, wherein the pH is at most 4.5.
89. the plant agent composition according to embodiment 88, wherein the pH is about 3.7 to about 4.2.
90. the plant agent composition according to any of embodiment 67-89, wherein the plant agent composition pair The mankind, mammal, plant and environment are generally nontoxic.
91. the plant agent composition according to any of embodiment 67-90, wherein the plant agent composition is Biodegradable.
92. it is a kind of control plant disease virulence factor method, the method includes it is following, be substantially made up of Or it is made up of：A effective amount of plant agent composition according to any of embodiment 67-91 definition is applied to and is caused The one or more of plants and/or the plant agent composition will be exposed to wherein described virulence factor that cause of disease infects Mode a effective amount of plant agent composition is applied to one or more places, wherein the application of the plant agent composition Lead to the adverse effect of the virulence factor to seeking controlled plant disease.
93. a kind of method for increasing plant growth and/or crop production, the method includes it is following, substantially by following It forms or is made up of：Using it is a effective amount of according to any of embodiment 67-91 definition plant agent composition and/or A effective amount of plant agent composition according to any of embodiment 7-91 definition is applied to wherein described plant agent to combine One or more places that object will be exposed to one or more of plants, wherein the application of the plant agent composition Lead to the improved absorption by root hair, improved xylem sap flowing by xylem and improved in bast Photosynthate flows, and the absorption of increased water, minerals and other nutrients from soil is increased in xylem In capillarity and/or hydrostatic pressing and/or increased compound and energy synthesis and/or blocking xylem sap stream The destruction of one or more of components of dynamic and/or photosynthate flowing.
Kept or the method for efficiency that improves the irrigation system 94. a kind of, the method includes it is following, substantially by with the following group Into or be made up of：A effective amount of plant agent composition according to any of embodiment 67-91 definition is applied to institute One or more pipelines in the pipeline network of irrigation system are stated, wherein the application of the plant agent composition causes blocking to fill Irrigate the suitable removing of one or more components of one or more pipeline networks of system.
95. a effective amount of plant agent composition according to defined in any of embodiment 67-91 is used to control plant The purposes of disease.
96. a effective amount of plant agent composition according to defined in any of embodiment 67-91 is used to increase plant Growth and/or the purposes of crop production.
97. a effective amount of plant agent composition according to defined in any of embodiment 67-91 is used to keep or change Into the purposes of the efficiency of irrigation system.
98. method according to embodiment 1,7-36 or 62-66 or according to embodiment 4,7-36 or 62-66 Purposes, wherein the plant disease is anthracnose, droop, canker, clubroot, plant decay, goiter and tumor, leaf blister, leaf Pinta, mildew, mycosis, mosaic virus, rot, rust, incrustation, smut, wilt disease.
99. method according to embodiment 1,7-36,62-66 or 98 or according to embodiment 4,7-36,62-66 or Purposes described in 98, wherein the virulence factor is the viral pathogenesis factor, bacterium virulence factor, the fungal attack factor, nematode cause Cause of disease or any combination thereof.
Embodiment
Following non-limiting example is provided for only illustrative purpose, is set now to help to be more fully understood The representative embodiment thought.These embodiments should not be construed as limited to appointing in the embodiment described in this specification What one, including those embodiments related with plant agent composition disclosed herein or method or purposes.
Embodiment 1
The preparation of the yeast supernatants 1 of processed fermentation
In order to prepare the yeast supernatants of processed fermentation, fermentation reaction is established, wherein will have at about 29 DEG C to about The warm water of the about 1,000L of temperature between 38 DEG C is placed in the mixing kettle of big jacketed.Xiang Shuizhong additions about 84.9kg's The magnesium sulfate of the untreated cane molasses of black, the raw sugar of about 25.2kg and about 1.2kg.Mixture is fully blended, later, Add the diastatic malt of about 11.4kg and the Saccharomyces cerevisiae of about 1.2kg and mild agitation.By mixture at about 26 DEG C to about 42 DEG C Incubation lasts about 3 days, and later, effervesce reaction is subsided, and instruction is substantially completely fermented.In fermentation ends, yeast is sent out Ferment composition centrifuges to remove " sludge " that is formed during fermentation.Collect the fermented supernatant fluid generated (by weight about 98.59%) and pass through high pressure sterilization and sterilize.Then the yeast supernatants of processed fermentation can be stored up in liquid form It deposits, for then using.Selectively, the yeast supernatants of processed fermentation can by method as known in the art come It is spray-dried to generate dried powder.Dry powder form can also be stored then to use.
Embodiment 2
The preparation of the yeast supernatants 2 of processed fermentation
In order to prepare the yeast supernatants of processed fermentation, fermentation reaction is established, wherein will have at about 29 DEG C to about The warm water of the about 1,000L of temperature between 38 DEG C is placed in the mixing kettle of big jacketed.Xiang Shuizhong additions about 42.5kg's The magnesium sulfate of the untreated cane molasses of black, the raw sugar of about 12.6kg and about 1.2kg.Mixture is fully blended, later, Add the diastatic malt of about 10.3kg and the Saccharomyces cerevisiae of about 1.2kg and mild agitation.By mixture at about 26 DEG C to about 42 DEG C Incubation lasts about 3 days, and later, effervesce reaction is subsided, and instruction is substantially completely fermented.In fermentation ends, yeast is sent out Ferment culture centrifuges to remove " sludge " that is formed during fermentation.Collect the fermented yeast supernatant generated (by weight about 98.59%) and pass through high pressure sterilization and handled.Then the yeast supernatants of processed fermentation can be stored up in liquid form It deposits, for then using.Selectively, the yeast supernatants of processed fermentation can by method as known in the art come It is spray-dried to generate dried powder.Dry powder form can also be stored then to use.
Embodiment 3
The preparation of the yeast supernatants 3 of processed fermentation
In order to prepare the yeast supernatants of processed fermentation, fermentation reaction is established, wherein will have at about 29 DEG C to about The warm water of the about 1,000L of temperature between 38 DEG C is placed in the mixing kettle of big jacketed.Xiang Shuizhong additions about 21.3kg's The magnesium sulfate of the untreated cane molasses of black, the raw sugar of about 6.3kg and about 1.2kg.Mixture is fully blended, later, Add the diastatic malt of about 9.3kg and the Saccharomyces cerevisiae of about 1.2kg and mild agitation.By mixture in about 26 DEG C to about 42 DEG C temperature It educates and lasts about 3 days, later, effervesce reaction has been weakened, and instruction is substantially completely fermented.In fermentation ends, yeast is fermented Culture centrifuges to remove " sludge " that is formed during fermentation.Collect the fermented supernatant fluid generated (by weight about 98.59%) and pass through high pressure sterilization and handled.Then the yeast supernatants of processed fermentation can be stored up in liquid form It deposits, for then using.Selectively, the yeast supernatants of processed fermentation can by method as known in the art come It is spray-dried to generate dried powder.Dry powder form can also be stored then to use.
Embodiment 4
The preparation of plant agent composition
In order to prepare plant agent composition, the hot sterile water (about 60 DEG C to about 65 DEG C) of 1,000L is pressed from both sides added to big band In the yeast supernatants of the processed fermentation of 1,000L in the mixture kettle of set.About 168.8kg is added into the mixture TERGITOLTMThe TERGITOL of 15-S-7 linear secondary alcohols ethoxylate, about 168.8kgTM15-S-5 linear secondary alcohol ethoxies The DOWFAX of glycolylate, about 67.5kgTM2A1 alkyl diphenyl ether disulfonates (alkyldiphenyloxide ) and the TRITON of about 67.5kg disulfonateTMH-66 phosphates polyether ester (phosphate polyether ester).The mixture is fully blended to generate solution.Then water is added, so that volume reaches about 4,5000L and stirs straight To being thoroughly mixed.The pH of the plant agent composition of generation is adjusted from about 3.7 to about 4.2 with phosphoric acid.Then, by pH The plant agent composition filtration sterilization of adjusting is to remove any microbial contamination.
The composition be found be to skin histology it is nonirritant, nontoxic and can within the period of several months quilt Cold position is stored in, without any perceptible loss of effectiveness or deterioration.
DOWFAXTM2A1 can be substituted, such as such as with following anionic biosurfactantAM 30-KE, Texapon Special,EHS, sodium 2-ethylhexyl sulfate or its group It closes.
Optionally, then the plant agent composition of generation can be mixed with following preservative or stabilizer, such as by weight Sodium benzoate, by weight about 0.01% imidazolidinyl urea, by weight about 0.15% two oxazolidinyls of gauge about 1% Urea, by weight about 0.25% calcium chloride.In the case of continuous agitation, addition sodium benzoate, imidazolidinyl urea, two oxazolidines Base urea and calcium chloride.Then, the temperature of mixture is slowly increased to about 40 DEG C and is continually stirred mixture.By temperature It is maintained at about 40 DEG C and lasts about 1 hour to ensure that all components of mixture are dissolved.Then, mixture is cooled down from about 20 DEG C To about 25 DEG C.The pH of the plant agent composition of generation is adjusted from about 3.7 to about 4.2 with phosphoric acid.Then, plant pH adjusted Agent composition filtration sterilization is to remove any microbial contamination.
Embodiment 5
The preparation of plant agent composition
In order to prepare plant agent composition, the hot sterile water (about 60 DEG C to about 65 DEG C) of 850L is placed on big jacketed Mixture kettle in.Powder, the about 37.5kg of the yeast supernatants drying of the processed fermentation of Xiang Shuizhong additions about 7.62g TERGITOLTM15-S-7 linear secondary alcohols ethoxylate), the TERGITOL of about 37.5kgTM15-S-5 linear secondary alcohol ethyoxyls The DOWFAX of compound, about 15.0kgTMThe TRITON of 2A1 alkyl diphenyl ether disulfonates and about 25.0kgTMH-66 phosphates Polyether ester.The mixture is fully blended to generate solution.Then water is added, so that volume reaches about 1,0000L and stirs straight To being thoroughly mixed.The pH of the plant agent composition of generation is adjusted from about 3.7 to about 4.2 with phosphoric acid.Then, by pH The plant agent composition filtration sterilization of adjusting is to remove any microbial contamination.
Optionally, then the plant agent composition of generation can be mixed with following preservative or stabilizer, such as by weight Sodium benzoate, by weight about 0.01% imidazolidinyl urea, by weight about 0.15% two oxazolidinyls of gauge about 1% Urea, by weight about 0.25% calcium chloride.In the case of continuous agitation, addition sodium benzoate, imidazolidinyl urea, two oxazolidines Base urea and calcium chloride.Then, the temperature of mixture is slowly increased to about 40 DEG C and is continually stirred mixture.By temperature It is maintained at about 40 DEG C and lasts about 1 hour to ensure that all components of mixture are dissolved.Then, mixture is cooled down from about 20 DEG C To about 25 DEG C.The pH of the plant agent composition of generation is adjusted from about 3.7 to about 4.2 with phosphoric acid.Then, plant pH adjusted Agent composition filtration sterilization is to remove any microbial contamination.
The composition be found be to skin histology it is nonirritant, nontoxic and can within the period of several months quilt Cold position is stored in, without any perceptible loss of effectiveness or deterioration.
DOWFAXTM2A1 can be substituted, such as such as with following anionic biosurfactantAM 30-KE, Texapon Special,EHS, sodium 2-ethylhexyl sulfate or its group It closes.
The optional object of powder that yeast supernatants as the processed fermentation disclosed in embodiment 1-3 are dried, can be with The powder dried using the yeast supernatants of commercially available processed fermentation, including for example154、210 or900。
Embodiment 6
Rice research
The implementation exemplifies effect of the plant agent composition disclosed herein to rice plants growth and cereal production.
In a kind of measure, plant agent composition disclosed herein is had evaluated to rice seed germination, seedling height and root The effect of length.Seed from rice varieties Oryzica 1 is divided into three groups, every group of 100 seeds are put into bag and every Group continues 2 minutes by the way that bag is immersed in pretreatment in one of following solution：1) distilled water (control)；2) plant disclosed herein 2% solution (supernatant and 8% surfactant of 92% processed fermentation) of agent composition；And 3) plant disclosed herein 2% solution (supernatant and 10% surfactant of 90% processed fermentation) of agent composition.The seed of processing is broadcast Then kind is placed on to be placed in soil cultivation device and continues 7-10 days to allow to germinate in sterile sand.By determining germination hundred Ratio, seedling height and root long degree is divided to assess germination effect.This, which is measured, carries out four times.As a result rice paddy seed kind is indicated The seed treatment of Oryzica 1 is in the variable of measurement：Difference is not shown in terms of germination percentage, seedling height and root long degree.
In another kind measures, effect of the plant agent composition disclosed herein to the growth of rice seedling is had evaluated.It will Seedling from rice varieties Oryzica 1 is divided into the group of 20 seedling, and is transplanted in seeder, wherein the soil to sterilize in advance Earth suitably applies fertilizer to meet the nutritional need well developed of rice plants.Seedling is divided into three groups, every group of five kinds of plants.Children With one kind in three kinds of solution of 2mL, processing continues 20 days to seedling daily.Group 1 is handled with distilled water (control)；Group 2, which is used, to be disclosed herein Plant agent composition 2% solution (supernatant and 8% surfactant of 92% processed fermentation) processing；And organize 3 At 2% solution (supernatant and 10% surfactant of 90% processed fermentation) of plant agent composition disclosed herein Reason.At the end of 20 day period, gathered data：Seedling height, stem production (stem production) and grain yield.
As a result indicate that, relative to control group 1, processing group 1 and 2 shows to be significantly improved.For example, a kind of assessment I, Stem nematode With the ratio of fringe (loose, branch flowers) growth.Control group 1 shows larger stem/fringe ratio (15.4/12.2), many Long stem has very short fringe, the instruction of less cereal production.Processing group 1 and processing group 2 show more favorable stem/fringe ratio (being respectively 10.4/7.4 and 12.2/10.2), this will lead to higher grain yield.Processing group 2 is shown relative to processing group 1 With better stem/fringe ratio, the long shoot with good fringe is generated.In addition, in the fringe of generation, control group 1 includes 13.5% Blank cereal (vain grain) (being not filled with rice grain), this is the instruction of sterility.Processing group 2 shows 6.4% sky White cereal, and processing group 2 shows 3.9% blank cereal.Therefore, plant is reduced with plant agent compositions-treated disclosed herein Sterility.In addition, when compared with control group 1, cereal of the processing group 2 per more than 14% plant production, and processing group 3 is often planted Object produces more than 64% cereal.In addition, when compared with control group 1, processing group 2 shows that 55% root growth increases, and locates The root growth that reason group 3 generates 104% increases.Generally speaking, 2% when rice plants plant agent composition disclosed herein is molten During liquid processing, apparent benefit is observed.
Embodiment 7
Tomato is studied
The implementation exemplifies the effect that plant agent composition disclosed herein produces tomato plants and fruit.
Tomato seedling is grown in comprising 40% husky and 60% organic compost soil in the greenhouse.Although not using fertilizer Material or urea, however using cow dung.Tomato seedling is divided into two groups：Control group includes being continued with the daily trickle irrigation of distilled water (control) The seedling of 6 months.Processing group is included with 1:Plant agent composition (the 90% processed fermentation disclosed herein of 400 dilutions Supernatant and 10% surfactant) trickle irrigation seedling.Based on monthly, assessment plant continues 6 months sections.Assessment Plant growth and fruit production.
As a result indicate that, relative to control group, processing group shows to be significantly improved.For example, processing group plant is entire whole 6 It is strongr than control group and more strong in a month section.In addition, for this research, control group has 30% death rate, and locates 100% survival rate (survivability rate) is presented in reason group.Continue in addition, processing group plant still blooms and bears fruit 5 months, and control group stopped blooming and bearing fruit after 3 months.It is compared finally, for the ultimate output of the crop of processing group 2 times or more is higher by according to group yield.For example, control group generates the tomato of 720Kg, and processing group generates the tomato of 1,715Kg.Total For, when 2% solution treatment of the tomato plants with plant agent composition disclosed herein, observe apparent benefit.
Similar research is carried out to parsley (parsley).As a result it indicates relative to the control group with distillation water process, it can be with The parsley more than five times is harvested from the processing group of 2% solution treatment with plant agent composition disclosed herein.
Similar research is carried out to eggplant.As a result it indicates relative to the control group with distillation water process, it can be from herein The processing group of 2% solution treatment of disclosed plant agent composition harvests more than 28% eggplant.
Embodiment 8
Chinese olive tree is studied
The implementation exemplifies effect of the plant agent composition disclosed herein to Chinese olive tree growth and fruit production.
The Chinese olive tree grown in fruit tree woods is divided into two groups：Control group uses distilled water (right during being included in two seasons According to) tree of trickle irrigation.Processing group is included in two seasons in the process with 1:The plant agent composition disclosed herein of 400 dilutions The tree of (supernatant and 10% surfactant of 90% processed fermentation) trickle irrigation.During research, based on monthly, comment Estimate plant.Assess plant growth and fruit production.
As a result indicate that, relative to control group, processing group shows to be significantly improved.For example, compared to control group, the production of processing group Raw about 35% to about 40% more olives.More surprisingly, compared with the control group every an annual bearing, for handling Chinese olive tree result in group occurs every year.Finally, the taste of oily taste particularly phenols is enhanced, and therefore relative to right According to group, the taste or quality of the oil of the tree in processing group are modified.
It carries out using 1:The other research of the plant agent composition disclosed herein of 1000 dilutions simultaneously obtains similar As a result.
Similar test result is observed with almond tree for walnut tree.
Embodiment 9
Tobacco is studied
The implementation exemplifies the effect that plant agent composition disclosed herein produces tobacco growing and fruit.
Tobacco seedling is grown in field.Tobacco seedling is divided into two groups：Control group includes daily with distilled water (control) Trickle irrigation continues the seedling of 6 months.Processing group is included with 1:(90% through place for the plant agent composition disclosed herein of 400 dilutions The supernatant of the fermentation of reason and 10% surfactant) trickle irrigation seedling.After being transplanted in field, processing group is included with 1: The seedling of the plant agent composition trickle irrigation disclosed herein of 800 dilutions.Based on monthly, when assessment plant continues 6 months Between section.Assess plant growth and fruit production.
As a result indicate that, relative to control group, processing group shows to be significantly improved.For example, compared to control group, from processing The tobacco plants of group show about 40% to about 50% more growths.In addition, the tobacco plants presentation from processing group is larger Root growth and preferable survival rate of seedling.
Similar test result is observed for hemp, although the research carries out in the greenhouse.
Embodiment 10
Plant disease is studied
The implementation exemplifies plant agent composition disclosed herein to treating the plant as caused by virulence factor disclosed herein The effect of object disease.
Using spray bottle, with 1 on plant agent composition disclosed herein:The processing of 200 dilutions has by fungal infection The rose of caused leaf spot.One to after two weeks, fungal infection disappears.
Using drip irrigation system, with 1 on plant agent composition disclosed herein:The processing of 1000 dilutions has is invaded by fungi The Chinese olive tree of droop caused by dye.One to after two weeks, fungal infection disappears.
Using drop drip irrigation system, with 1 on plant agent composition disclosed herein:The processing of 1000 dilutions has by bacterium The Chinese olive tree of droop caused by infecting.One to after two weeks, bacterial invasion disappears.
Finally, it will be understood that although highlighting the various aspects of this specification by reference to specific embodiment, The person skilled in the art will easily understand the principles of these disclosed embodiments only illustration presently disclosed subject matter.Therefore, It should be appreciated that unless clearly similary statement, otherwise disclosed theme is never limited to specific compound described herein, group Close object, article, equipment, method, scheme and/or reagent etc..In addition, it will be appreciated by the skilled addressee that without departing from this In the case of the spirit of specification, according to the teaching of this article, certain variations can be made, modification, arrangement, changes, addition, reduce And its sub-portfolio.Accordingly, it is anticipated that claim appended below and the claim introduced from now on be interpreted as including it is all this Variation, modification, arrangement, change, addition, reduction and its sub-portfolio of sample, because in its real spirit and scope.
This document describes certain embodiments of the present invention, implement the best of the present invention including being used for known to the present inventor Pattern.Certainly, after foregoing description is read, about these description embodiments modification for this field ordinary skill It will be apparent for personnel.The inventors expect that technical staff is optionally using such modification, and the present inventor is intended to The present invention is carried out in a manner of otherwise than as specifically described herein.Therefore, the present invention include as applicable law it is permitted The all modifications and equivalent of the theme described in this appended claims.In addition, unless in addition indicating herein or with it He is apparently contradicted in the context mode, and otherwise any combinations of above-described embodiment are with its all possible modification quilt sheet Invention is covered.
The grouping of the optional embodiment, element or step of the present invention is not interpreted to limit.Each group membership can be Individually or with other group memberships disclosed herein referred to and be claimed in combination.For convenience and/or patentability The reason of, it is contemplated that one or more members in group can be included in group or be deleted from group.When generation is any in this way When including or deleting, this specification is considered including the group of modification, all so as to fulfill what is used in the appended claims The written description of Markush (Markush) group.
Unless otherwise instructed, the expression characterization that otherwise uses in the present specification and claims, project, quantity, ginseng All numbers of number, property, term etc. are understood to be modified by term " about " in all cases.As used herein, art Language " about " mean so quantitative characteristic, project, quantity, parameter, property or term cover above and below the characteristic stated, Project, quantity, parameter, positive or negative 10 range of the value of property or term.Therefore, unless the contrary indication, otherwise The numerical parameter stated in this specification and in the appended claims is the approximation that can change.For example, determining to give During the quality of analyte, mass spectroscopy device can slightly change, the quality of ion or matter/lotus of ion than context in, Term " about " refers to +/- 0.50 atomic mass unit.At least, without be attempt to limitation the scope of the claims doctrine of equivalents Application, each numerical value should according at least to the effective digital of report number and by application common rounding-off technology come It explains.
The use of term " may (may) " in terms of embodiment or embodiment or " can (can) " also band There is the replacement meaning of " may not (may not) " or " cannot (cannot) ".Therefore, if subject description discloses embodiments Embodiment aspect may (may) or can (can) as subject of the present invention part by including, then also clearly Mean negative limitation or removing property condition, it is meant that may not be in terms of embodiment or embodiment or cannot function as this hair The part of bright theme by including.In a similar way, the term " optionally " in terms of embodiment or embodiment Using mean such embodiment or embodiment for the use of possibly as subject of the present invention part by including or can Can not as subject of the present invention part by including.Whether application will be born based on this for such negative limitation or removing property condition Whether face being limited or precluded property condition is enumerated in theme claimed.
Although the broad range of numberical range and value of stating the present invention are approximation, state in a particular embodiment Numberical range and value are reported as precisely as possible.However, any numberical range or value inherently include certain errors, the error The inevitable standard deviation by being found in being measured in its respective test generates.Herein to the numberical range of value enumerate only by It is intended as individually referring to the convenient method for falling into each independent numerical value in the range.Unless in addition instruction herein, no Then each individual value of numberical range is incorporated into this specification, as it is individually enumerated herein.
Unless it in addition indicates or is apparently contradicted in the context herein, otherwise in the context describing the invention (particularly In the context of the appended claims) term " one (a) " that uses, " one (an) ", " should (the) " and similar instruction Word is interpreted both covering odd number and plural number.In addition, ordinal indicators such as " first " for the element of identification, " Two ", " third " etc. is used to distinguish between these elements, and needs or limitation the numbers of element as not indicating or implying Mesh, and unless in addition specifically state, specific position or the sequence of such element are not otherwise indicated.Institute described herein There is method that can carry out in any suitable order, unless in addition indicating or being in addition apparently contradicted in the context herein.It carries herein For any and whole embodiments or exemplary language (for example, " such as ") use be intended merely to be better described the present invention and The range of otherwise claimed invention is not caused to limit.Language in this specification is not construed as referring to Show and essential any element being not claimed is put into practice to the present invention.
When used in a claim, it either submits or adds according to amendment, open transitional term " includes (comprising) " (and its equivalent open transitional phrase is as including (including), included (containing) and tool Have) individually or the theme with not describing covers all elements clearly described, limitation, step and/or feature in combination；Institute Element, limitation and/or the feature pointed out are necessary, but can add other element, limitation and/or features for not pointing out, And still form the construction in the scope of the claims.Closed transitional phrase can be used in the claims " by ... group Into " or " substantially by ... form " substitute or further limit public affairs herein as the modification to " comprising (comprising) " The specific embodiment opened.When in detail in the claims in use, either according to amendment submit or add, it is closed Transitional phrase " by ... form " exclude any element, limitation, step or the feature do not enumerated clearly in the claims.Envelope The scope of the claims is limited to the element clearly enumerated, limitation, step by enclosed transitional phrase " substantially by ... form " Any other element of rapid and/or feature and non-materially affect theme claimed fundamental characteristics and novel characteristics, Limitation, step and/or feature.Therefore, the meaning of open transitional phrase " including (comprising) " is defined as covering institute There are the element specifically enumerated, limitation, step and/or feature and any optional, in addition unspecified element, limitation, step Rapid and/or feature.Closed transitional phrase " by ... form " meaning be defined as only including specifically arranging in claim Those elements, limitation, step and/or the feature lifted, and the meaning quilt of closed transitional phrase " substantially by ... form " It is defined as those elements, limitation, step and/or the feature that only include specifically enumerating in claim and non-materially affect is wanted Ask the fundamental characteristics of the theme of protection and those elements, limitation, step and/or the feature of novel characteristics.Therefore, open transition Wording " includes (comprising) " (and its equivalent open transitional phrase) and includes in its meaning as limitation implementations By closed transitional phrase " by ... form " or " substantially by ... form " claimed theme for specifying.Cause This, embodiment described herein or being claimed in this way with wording " including (comprising) " is by clearly or intrinsic bright Describe in vain, can realize and support herein phrase " substantially by ... form " and " by ... form ".
The composition described in these publications that can be for example used in conjunction with the invention for descriptions and disclosures and side The purpose of the science of law, all patents, patent publications and other publications quoted and indicated in this specification are by independent and bright Really it is integrally incorporated by quoting with it.These publications are provided just for the sake of its disclosure before the submitting day of the application Content.Nothing should be construed as an admission that inventor haves no right by previous invention or due to any other original in this regard Because open prior to as.The narration on all about date or statement about these literature contents can be obtained based on applicant Information, and any of correctness for not forming the date or content about these documents recognizes.
Finally, terms used herein are only used for the purpose of description particular embodiment, and are not intended to limit the present invention's Range, the scope of the present invention are limited only by the appended claims.Therefore, the present invention be inaccurately limited to as shown with it is described Like that.

Claims (29)

1. a kind of plant agent composition, the microorganism supernatant comprising processed fermentation and one or more of non-ionic tables Face activating agent, wherein the composition does not have the bacterium of any organized enzyme or work, and wherein described composition has less than 5.0 PH.
2. plant agent composition according to claim 1, wherein the microorganism supernatant of the processed fermentation comes from The yeast supernatants of fermentation, the bacterial supernatant to ferment, the mould supernatant to ferment or any combination thereof.
3. plant agent composition according to claim 2, wherein the yeast supernatants of the fermentation are by belonging to following category Yeast species generate：Brettanomyce Pseudomonas, intends Weir Blastocystis, the black powder Blastocystis of sporangiocyst line, moral at Candida Bali Blastocystis, moral carat Blastocystis, Fusarium, geotrichum sp category, her Sa Blastocystis, Kazakhstan's saccharomycete Category, Ke Leke Blastocystis, Kluyveromyces, lecanium Pseudomonas, Mucor, Neurospora, Pediococcus, Penicillium notatum Category, Pichia pastoris Pseudomonas, Rhizopus, rhodosporidium toruloides Pseudomonas, rhodotorula Pseudomonas, Blastocystis, Schizosccharomyces, hair spore Sub- Blastocystis has spore Torula, Torulopsis, Verticillium dahliae category, Ye Luoweiya Blastocystis, Zygosaccharomyces Pseudomonas Or Zygotorulaspora.
4. plant agent composition according to claim 3, wherein the yeast supernatants of the fermentation are by yeast S. cerevisiae It generates.
5. the plant agent composition according to any one of claim 1-4, wherein the plant agent composition is included by weight The microorganism supernatant of the processed fermentation of gauge at least 35%.
6. plant agent composition according to any one of claims 1-5, wherein the plant agent composition is included by weight The microorganism supernatant of the processed fermentation of gauge at most 95%.
7. the plant agent composition according to any one of claim 1-6, wherein the nonionic surface active agent packet Include polyethers nonionic surface active agent, polyhydroxy nonionic surface active agent and/or biosurfactant.
8. plant agent composition according to claim 7, wherein the polyhydroxy nonionic surface active agent includes sugarcane Sugar ester, the sucrose ester of ethoxylation, sorbitol ester, the sorbitol ester of ethoxylation, alkyl-glucoside, ethoxylation Alkyl-glucoside, polyglycerol ester or the polyglycerol ester of ethoxylation.
9. the plant agent composition according to any one of claim 1-8, wherein the nonionic surface active agent packet Include amine oxide, the alcohol of ethoxylation, the aliphatic alcohol of ethoxylation, alkylamine, the alkylamine of ethoxylation, ethoxylation Alkylphenol, alkyl polysaccharide, the alkyl polysaccharide of ethoxylation, the aliphatic acid of ethoxylation, ethoxylation fatty alcohol or ethoxy The fatty amine of base has H (OCH2CH2)xOC6H4R1、(OCH2CH2)xOR2Or H (OCH2CH2)xOC(O)R2General formula it is non- The expression of ionic surfactant, wherein x is added into mole of the ethylene oxide of alkylphenol and/or fatty alcohol or aliphatic acid Number, R1Represent long chain alkyl group and R2Represent long aliphatic radicals.
10. plant agent composition according to claim 9, wherein R1It is C7-C10The alkyl group and/or wherein R of positive structure2 It is C12-C20Aliphatic group.
11. the plant agent composition according to any one of claim 1-10, wherein the nonionic surface active agent Be the nonyl phenol of ethoxylation, the octyl phenol of ethoxylation, the cetyl oleyl alcohol of ethoxylation, ethoxylation cetyl Stearyl alcohol, the decyl alcohol of ethoxylation, the lauryl alcohol of ethoxylation, the tridecanol of ethoxylation or the castor oil of ethoxylation.
12. the plant agent composition according to any one of claim 1-11 is pressed wherein the plant agent composition includes One or more of nonionic surface active agent of weight meter from about 1% to about 15%.
13. plant agent composition according to claim 12, wherein the plant agent composition is included by weight from about 5% to about 13% one or more of nonionic surface active agent.
14. plant agent composition according to claim 13, wherein the plant agent composition is included by weight from about 7% to about 11% one or more of nonionic surface active agent.
15. the plant agent composition according to any one of claim 1-14, wherein the plant agent composition also includes One or more of anionic surfactant.
16. plant agent composition according to claim 15, wherein the plant agent composition is included by weight from about 0.5% to about 10% one or more of anionic surfactant.
17. plant agent composition according to claim 16, wherein the plant agent composition is included by weight from about 1% to about 8% one or more of anionic surfactant.
18. plant agent composition according to claim 17, wherein the plant agent composition is included by weight from about 2% to about 6% one or more of anionic surfactant.
19. the plant agent composition according to any one of claim 1-18, wherein the pH is at most 4.5.
20. plant agent composition according to claim 19, wherein the pH is about 3.7 to about 4.2.
21. the plant agent composition according to any one of claim 1-20, wherein the plant agent composition also includes Antimicrobial.
22. the plant agent composition according to any one of claim 1-21, wherein the plant agent composition to the mankind, Mammal, plant and environment are generally nontoxic.
23. the plant agent composition according to any one of claim 1-22, wherein the plant agent composition is can to give birth to Object degradation.
24. it is a kind of control plant disease virulence factor method, the method includes by a effective amount of according to claim 1- The plant agent compositions of any one of 23 definition are applied to one or more of plants for being infected by virulence factor and/or with wherein The virulence factor will be any in a effective amount of 1-23 according to claim by the mode for being exposed to the plant agent composition The plant agent composition of item definition is applied to one or more places, wherein the application of the plant agent composition causes to seeking Seek the adverse effect of the controlled virulence factor.
25. a kind of method for increasing plant growth and/or crop production, the method includes by a effective amount of according to claim The plant agent composition of any one of 1-23 definition is applied to one or more of plants and/or is wanted a effective amount of according to right Ask the plant agent composition that any one of 1-23 is defined be applied to wherein plant agent composition will be exposed to it is described a kind of or more One or more places of various plants, wherein the application of the plant agent composition leads to the improved suction by root hair It receives, it is improved to be flowed by the xylem sap flowing of xylem and the improved photosynthate in bast, increase Water, minerals and other nutrients from soil absorption, increased capillarity in xylem and/or quiet The synthesis and/or the flowing of blocking xylem sap of hydraulic pressure and/or increased compound and energy and/or photosynthate stream The destruction of dynamic one or more of components.
Kept or the method for efficiency that improves the irrigation system 26. a kind of, the method includes by a effective amount of according to claim The plant agent composition of any one of 1-23 definition is applied to one or more pipes in the pipeline network of the irrigation system Road, wherein the application of the plant agent composition leads to the one kind or more for stopping one or more pipeline networks of irrigation system The suitable removing of various ingredients.
27. a effective amount of plant agent composition according to defined in any one of claim 1-23 is used to control plant disease Purposes.
28. a effective amount of plant agent composition according to defined in any one of claim 1-23 is used to increase plant growth And/or the purposes of crop production.
29. a effective amount of plant agent composition according to defined in any one of claim 1-23 is used to keeping or improving irrigation The purposes of the efficiency of system.
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