CN108117932A - The recovery processing technique of tuna leftover bits and pieces - Google Patents
The recovery processing technique of tuna leftover bits and pieces Download PDFInfo
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- CN108117932A CN108117932A CN201711412875.2A CN201711412875A CN108117932A CN 108117932 A CN108117932 A CN 108117932A CN 201711412875 A CN201711412875 A CN 201711412875A CN 108117932 A CN108117932 A CN 108117932A
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- carbon dioxide
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- liquid
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- 238000012545 processing Methods 0.000 title claims abstract description 81
- 238000000034 method Methods 0.000 title claims abstract description 42
- 238000011084 recovery Methods 0.000 title claims abstract description 22
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 136
- 239000007788 liquid Substances 0.000 claims abstract description 86
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 72
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 68
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 67
- 239000002002 slurry Substances 0.000 claims abstract description 49
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 36
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 claims abstract description 25
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims abstract description 18
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 17
- 102000057297 Pepsin A Human genes 0.000 claims abstract description 17
- 108090000284 Pepsin A Proteins 0.000 claims abstract description 17
- 229940111202 pepsin Drugs 0.000 claims abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 17
- 230000005684 electric field Effects 0.000 claims abstract description 10
- 238000002525 ultrasonication Methods 0.000 claims description 24
- 239000004365 Protease Substances 0.000 claims description 17
- 108090000526 Papain Proteins 0.000 claims description 16
- 102000005158 Subtilisins Human genes 0.000 claims description 16
- 108010056079 Subtilisins Proteins 0.000 claims description 16
- 235000019834 papain Nutrition 0.000 claims description 15
- 229940055729 papain Drugs 0.000 claims description 15
- 102000004190 Enzymes Human genes 0.000 claims description 13
- 108090000790 Enzymes Proteins 0.000 claims description 13
- 238000005119 centrifugation Methods 0.000 claims description 13
- 229940088598 enzyme Drugs 0.000 claims description 13
- 239000012263 liquid product Substances 0.000 claims description 7
- 210000000496 pancreas Anatomy 0.000 claims description 3
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 claims 1
- 102000002322 Egg Proteins Human genes 0.000 claims 1
- 108010000912 Egg Proteins Proteins 0.000 claims 1
- 235000014103 egg white Nutrition 0.000 claims 1
- 210000000969 egg white Anatomy 0.000 claims 1
- 235000021323 fish oil Nutrition 0.000 abstract description 29
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 31
- 241000251468 Actinopterygii Species 0.000 description 8
- 238000000926 separation method Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 4
- 238000002604 ultrasonography Methods 0.000 description 4
- 239000007789 gas Substances 0.000 description 3
- 102000035195 Peptidases Human genes 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 235000019198 oils Nutrition 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 235000019419 proteases Nutrition 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 239000002253 acid Substances 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000002785 anti-thrombosis Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 239000002781 deodorant agent Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000007180 physiological regulation Effects 0.000 description 1
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000007127 saponification reaction Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000004408 titanium dioxide Substances 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B13/00—Recovery of fats, fatty oils or fatty acids from waste materials
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/025—Pretreatment by enzymes or microorganisms, living or dead
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/50—Reuse, recycling or recovery technologies
- Y02W30/74—Recovery of fats, fatty oils, fatty acids or other fatty substances, e.g. lanolin or waxes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Meat, Egg Or Seafood Products (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a kind of recovery processing technique of tuna leftover bits and pieces, including：Step 1) is cleaned up tuna leftover bits and pieces with clear water, is smashed；Step 2) adds in pepsin into slurry and carries out primary enzymolysis, is continually fed into nitrogen, the flow of nitrogen is 1.5 2.5m/s, obtains primary enzymolysis liquid；Step 3) is continually fed into carbon dioxide into primary enzymolysis liquid, is placed in high-pressure pulse electric and is handled, and continues to be passed through carbon dioxide, the electric field strength of high-pressure pulse electric is 55 60kV/cm；Step 4) adds neutral proteinase and carries out secondary enzymolysis, is continually fed into nitrogen, the flow of nitrogen is 3.5 5.5m/s；Step 5) adds in hydrochloric acid into secondary enzymolysis liquid, continuously adds absolute ethyl alcohol；Step 6) centrifuges.The present invention improves the recovery rate to fish oil, improves the content of EPA and DHA in fish oil.
Description
Technical field
The present invention relates to agriculture field more particularly to a kind of recovery processing techniques of tuna leftover bits and pieces.
Background technology
Fish is one of important aquatic resources in China, and yield occupies first place in the world.But many fish process are only limited to
In the utilization to fish body muscle, and it is less to the processing and utilization of the byproducts such as fish guts, fish-bone, fish head, generally as discarded object
Processing.The integral level of China's comprehensive utilization fish pomace is not also high, often the by-product containing higher protein and fat content
How effectively product do not obtain rationally effective performance, this not only causes the waste of resource, but also polluted environment, so profit
With these resources, turn waste into wealth, will be a very important research topic.Contain relatively rich unsaturated lipid in marine products fish oil
Fat acid, main component are EPA and DHA, and polyunsaturated fatty acid has anti-inflammatory, antithrombotic, antitumor and promotion brain hair
It educates, enhance a variety of physiological regulation functions such as memory.The domestic fish oil of extraction at present is mostly using saponification method, molecularly distilled, urine
The single methods such as plain inclusion method, CO2 methods extract fish oil, in terms of purity and oil yield all there are it is respective the shortcomings that；In addition it is international
On the fish oil deodorant problem that processes also be not well solved.Therefore, these fish pomaces how are made full use of, most
The grease for therefrom extracting high quality of big degree, the added value for improving fish processing, reduction environmental pollution become current important
Problem.
The content of the invention
It is an object of the invention to solve at least the above, and provide the advantages of at least will be described later.
It is a still further object of the present invention to provide a kind of recovery processing technique of tuna leftover bits and pieces, including：
Step 1) is cleaned up tuna leftover bits and pieces with clear water, is smashed, and addition is equivalent to the tuna leftover bits and pieces
0.5-2 times of water is mixed into slurry, and the slurry is stirred evenly；
Step 2) adds in pepsin into the slurry that the step 1) is prepared and carries out primary enzymolysis, primary enzymolysis
Temperature is 30-35 DEG C, pH value 2-3, when the primary enzymolysis time is 9-10 small, wherein, the addition of the pepsin is institute
The 2-2.5% for the slurry quality that step 1) is prepared is stated, during primary enzymolysis, while is continued into the slurry
Nitrogen is passed through, the flow of nitrogen is 1.5-2.5m/s, obtains primary enzymolysis liquid；
The primary enzymolysis liquid obtained by the step 2) processing is placed in a container by step 3), to the primary enzymolysis
Carbon dioxide is continually fed into liquid, is passed through the time of carbon dioxide as 1-2h, the flow of carbon dioxide is 4-
The container is placed in high-pressure pulse electric handles afterwards by 5m/s, in the high-pressure pulse electric processing procedure, after
Continuous to be passed through carbon dioxide, the flow of carbon dioxide is 1-2m/s, after the high-pressure pulse electric processing terminates,
It also continues to be passed through carbon dioxide, the flow of carbon dioxide is protected with the flow in the high-pressure pulse electric processing procedure
It holds unanimously, the electric field strength of the high-pressure pulse electric is 55-60kV/cm, frequency 100-150Hz, pulse width 20-25
μ s, processing time 5-10s；
The feed liquid that the step 3) processing obtains is placed 30-60min by step 4), and it is secondary to add neutral proteinase progress
Enzymolysis, secondary enzymolysis temperature are 40-45 DEG C, pH value 8-9, and the addition of the neutral proteinase is handled for the step 3)
The 2-3% of the feed liquid quality arrived, when the secondary enzymolysis time is 3-4 small, during secondary enzymolysis, while into the slurry
Nitrogen is continually fed into, the flow of nitrogen is 3.5-5.5m/s, obtains secondary enzymolysis liquid；Wherein, the neutral proteinase is by pancreas
Protease, papain and subtilopeptidase A, the matter of the trypsase, papain and subtilopeptidase A
Amount is than being 4-5:1-2:0.5-1；
Step 5) adds in the hydrochloric acid that mass concentration is 2-3% into the secondary enzymolysis liquid, by the secondary enzymolysis liquid
PH is adjusted to 4-5, continuously adds absolute ethyl alcohol, and addition of the absolute ethyl alcohol in the secondary enzymolysis liquid is described secondary
The 65-70% of enzymolysis liquid volume, when standing 20-23 is small；
The feed liquid that the step 5) is prepared is placed in centrifugation apparatus and is centrifuged by step 6), and centrifuge RPMs are
8000-9000r/min, the time of centrifugal treating is 26-33min.
Preferably, in the recovery processing technique of the tuna leftover bits and pieces, in the step 2), in primary enzymolysis mistake
Cheng Zhong is carried out at the same time ultrasonication, and the supersonic frequency of the ultrasonication is 800-950kHz.
Preferably, in the recovery processing technique of the tuna leftover bits and pieces, in the step 2), in primary enzymolysis mistake
Cheng Zhong, the supersonic frequency of the ultrasonication is 800kHz.
Preferably, in the recovery processing technique of the tuna leftover bits and pieces, in the step 4), in secondary enzymolysis mistake
Cheng Zhong is carried out at the same time ultrasonication, and the supersonic frequency of the ultrasonication is 650-700kHz.
Preferably, in the recovery processing technique of the tuna leftover bits and pieces, in the step 4), at the ultrasonic wave
The supersonic frequency of reason is 700kHz.
Preferably, in the recovery processing technique of the tuna leftover bits and pieces, in the step 3), to an enzyme
Carbon dioxide is continually fed into solution liquid, is passed through the time of carbon dioxide as 1h, the flow of carbon dioxide is 4m/
S in the high-pressure pulse electric processing procedure, continues to be passed through carbon dioxide, the flow of carbon dioxide is 1m/s.
The present invention includes at least following advantageous effect：
A kind of recovery processing technique of tuna leftover bits and pieces provided by the invention, including：Step 1) is with clear water by tuna
Leftover bits and pieces cleans up, and smashes, and the water that addition is equivalent to 0.5-2 times of the tuna leftover bits and pieces is mixed into slurry, by described in
Slurry stirs evenly；Step 2) adds in pepsin into the slurry that the step 1) is prepared and carries out primary enzymolysis,
Primary enzymolysis temperature is 30-35 DEG C, pH value 2-3, when the primary enzymolysis time is 9-10 small, wherein, the pepsin adds
Enter the 2-2.5% for the slurry quality that amount is prepared for the step 1), during primary enzymolysis, while to the pulpous state
Nitrogen is continually fed into object, the flow of nitrogen is 1.5-2.5m/s, obtains primary enzymolysis liquid；Step 3) will pass through the step 2)
It handles obtained primary enzymolysis liquid to be placed in a container, is continually fed into carbon dioxide into the primary enzymolysis liquid, is passed through
The time of carbon dioxide is 1-2h, and the flow of carbon dioxide is 4-5m/s, and the container is placed in high-tension pulse afterwards
It rushes in electric field and is handled, in the high-pressure pulse electric processing procedure, continue to be passed through carbon dioxide, carbon dioxide gas
The flow of body is 1-2m/s, after the high-pressure pulse electric processing terminates, also continues to be passed through carbon dioxide, titanium dioxide
The flow of carbon gas is consistent with the flow in the high-pressure pulse electric processing procedure, the electric field of the high-pressure pulse electric
Intensity is 55-60kV/cm, and frequency 100-150Hz, pulse width is 20-25 μ s, processing time 5-10s；Step 4) by institute
It states the feed liquid that step 3) processing obtains and places 30-60min, add neutral proteinase and carry out secondary enzymolysis, secondary enzymolysis temperature
For 40-45 DEG C, pH value 8-9, the addition of the neutral proteinase handles the 2- of obtained feed liquid quality for the step 3)
3%, when the secondary enzymolysis time is 3-4 small, during secondary enzymolysis, while nitrogen, nitrogen are continually fed into the slurry
The flow of gas is 3.5-5.5m/s, obtains secondary enzymolysis liquid；Wherein, the neutral proteinase is by trypsase, Papain
Enzyme and subtilopeptidase A, the mass ratio of the trypsase, papain and subtilopeptidase A is 4-5:1-2:
0.5-1；Step 5) adds in the hydrochloric acid that mass concentration is 2-3% into the secondary enzymolysis liquid, by the pH of the secondary enzymolysis liquid
It adjusts to 4-5, continuously adds absolute ethyl alcohol, addition of the absolute ethyl alcohol in the secondary enzymolysis liquid is the secondary enzyme
The 65-70% of liquid product is solved, when standing 20-23 is small；The feed liquid that the step 5) is prepared is placed in centrifugation apparatus by step 6)
In be centrifuged, centrifuge RPMs 8000-9000r/min, time of centrifugal treating is 26-33min.
The present invention carries out the slurry prepared by tuna leftover bits and pieces primary enzymolysis, high-pressure pulse electric is handled, secondary
Enzymolysis, on the one hand improves the recovery rate to fish oil, on the other hand reduces the destruction to EPA and DHA, and it is final to improve the two
Content in fish oil；And simultaneously using ultrasonication during primary enzymolysis and secondary enzymolysis, to improve enzymolysis
Efficiency, finally improve the recovery rate of fish oil.
The present invention improves the recovery rate to fish oil, improves the content of EPA and DHA in fish oil.
Part is illustrated to embody by further advantage, target and the feature of the present invention by following, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Specific embodiment
The present invention is described in further detail below, to make those skilled in the art being capable of evidence with reference to specification word
To implement.
It should be appreciated that such as " having ", "comprising" and " comprising " term used herein do not allot one or more
The presence or addition of a other elements or its combination.
Embodiment 1
Step 1) is cleaned up tuna leftover bits and pieces with clear water, is smashed, and addition is equivalent to the tuna leftover bits and pieces 0.5
Water again is mixed into slurry, and the slurry is stirred evenly；
Step 2) adds in pepsin into the slurry that the step 1) is prepared and carries out primary enzymolysis, primary enzymolysis
Temperature is 30 DEG C, pH value 2, when the primary enzymolysis time is 9 small, wherein, the addition of the pepsin is the step 1)
The 2% of the slurry quality being prepared during primary enzymolysis, is carried out at the same time ultrasonication, the ultrasonication
Supersonic frequency for 800kHz, while nitrogen is continually fed into the slurry, the flow of nitrogen is 1.5m/s, is obtained once
Enzymolysis liquid；
The primary enzymolysis liquid obtained by the step 2) processing is placed in a container by step 3), to the primary enzymolysis
Carbon dioxide is continually fed into liquid, is passed through the time of carbon dioxide as 1h, the flow of carbon dioxide is 4m/s,
The container is placed in high-pressure pulse electric afterwards and is handled, in the high-pressure pulse electric processing procedure, continues to lead to
Enter carbon dioxide, the flow of carbon dioxide is 1m/s, after the high-pressure pulse electric processing terminates, is also continued to
Carbon dioxide is passed through, the flow of carbon dioxide keeps one with the flow in the high-pressure pulse electric processing procedure
It causes, the electric field strength of the high-pressure pulse electric is 55kV/cm, and frequency 100Hz, pulse width is 20 μ s, and processing time is
5s；
The feed liquid that the step 3) processing obtains is placed 30min by step 4), is added neutral proteinase and is carried out secondary enzyme
Solution, secondary enzymolysis temperature are 40 DEG C, and pH value 8, the addition of the neutral proteinase handles obtained material for the step 3)
The 2% of liquid quality when the secondary enzymolysis time is 3 small, during secondary enzymolysis, is carried out at the same time ultrasonication, the ultrasound
The supersonic frequency of ripple processing is 650kHz, while nitrogen is continually fed into the slurry, and the flow of nitrogen is 3.5m/s, is obtained
To secondary enzymolysis liquid；Wherein, the neutral proteinase be by trypsase, papain and subtilopeptidase A, it is described
The mass ratio of trypsase, papain and subtilopeptidase A is 4:1:0.5；
Step 5) adds in the hydrochloric acid that mass concentration is 2% into the secondary enzymolysis liquid, by the pH of the secondary enzymolysis liquid
It adjusts to 4-5, continuously adds absolute ethyl alcohol, addition of the absolute ethyl alcohol in the secondary enzymolysis liquid is the secondary enzyme
The 65% of liquid product is solved, when standing 20 is small；
The feed liquid that the step 5) is prepared is placed in centrifugation apparatus and is centrifuged by step 6), and centrifuge RPMs are
8000r/min, the time of centrifugal treating is 26min；
Step 7) is handled in obtained fish oil with the step 6) and adds in activated carbon, and the addition of the activated carbon is described
The 3% of the fish oil quality that step 6) processing obtains；Again using centrifugation apparatus by Activated carbon separation, so as to obtain fish oil.
Embodiment 2
Step 1) is cleaned up tuna leftover bits and pieces with clear water, is smashed, and addition is equivalent to 2 times of the tuna leftover bits and pieces
Water be mixed into slurry, the slurry is stirred evenly；
Step 2) adds in pepsin into the slurry that the step 1) is prepared and carries out primary enzymolysis, primary enzymolysis
Temperature is 35 DEG C, pH value 3, when the primary enzymolysis time is 10 small, wherein, the addition of the pepsin is the step 1)
The 2.5% of the slurry quality being prepared during primary enzymolysis, is carried out at the same time ultrasonication, at the ultrasonic wave
The supersonic frequency of reason is 950kHz, while nitrogen is continually fed into the slurry, and the flow of nitrogen is 2.5m/s, obtains one
Secondary enzymolysis liquid；
The primary enzymolysis liquid obtained by the step 2) processing is placed in a container by step 3), to the primary enzymolysis
Carbon dioxide is continually fed into liquid, is passed through the time of carbon dioxide as 2h, the flow of carbon dioxide is 5m/s,
The container is placed in high-pressure pulse electric afterwards and is handled, in the high-pressure pulse electric processing procedure, continues to lead to
Enter carbon dioxide, the flow of carbon dioxide is 2m/s, after the high-pressure pulse electric processing terminates, is also continued to
Carbon dioxide is passed through, the flow of carbon dioxide keeps one with the flow in the high-pressure pulse electric processing procedure
It causes, the electric field strength of the high-pressure pulse electric is 60kV/cm, and frequency 150Hz, pulse width is 25 μ s, and processing time is
10s；
The feed liquid that the step 3) processing obtains is placed 60min by step 4), is added neutral proteinase and is carried out secondary enzyme
Solution, secondary enzymolysis temperature are 45 DEG C, and pH value 9, the addition of the neutral proteinase handles obtained material for the step 3)
The 3% of liquid quality when the secondary enzymolysis time is 4 small, during secondary enzymolysis, is carried out at the same time ultrasonication, the ultrasound
The supersonic frequency of ripple processing is 700kHz, while nitrogen is continually fed into the slurry, and the flow of nitrogen obtains for 5.5m/s
To secondary enzymolysis liquid；Wherein, the neutral proteinase be by trypsase, papain and subtilopeptidase A, it is described
The mass ratio of trypsase, papain and subtilopeptidase A is 5:2:1；
Step 5) adds in the hydrochloric acid that mass concentration is 3% into the secondary enzymolysis liquid, by the pH of the secondary enzymolysis liquid
It adjusts to 4-5, continuously adds absolute ethyl alcohol, addition of the absolute ethyl alcohol in the secondary enzymolysis liquid is the secondary enzyme
The 70% of liquid product is solved, when standing 23 is small；
The feed liquid that the step 5) is prepared is placed in centrifugation apparatus and is centrifuged by step 6), and centrifuge RPMs are
9000r/min, the time of centrifugal treating is 33min；
Step 7) is handled in obtained fish oil with the step 6) and adds in activated carbon, and the addition of the activated carbon is described
The 4% of the fish oil quality that step 6) processing obtains；Again using centrifugation apparatus by Activated carbon separation, so as to obtain fish oil.
Embodiment 3
Step 1) is cleaned up tuna leftover bits and pieces with clear water, is smashed, and is added in and is equivalent to 2 times of the tuna leftover bits and pieces
Water is mixed into slurry, and the slurry is stirred evenly；
Step 2) adds in pepsin into the slurry that the step 1) is prepared and carries out primary enzymolysis, primary enzymolysis
Temperature is 35 DEG C, pH value 3, when the primary enzymolysis time is 10 small, wherein, the addition of the pepsin is the step 1)
The 2.5% of the slurry quality being prepared during primary enzymolysis, is carried out at the same time ultrasonication, at the ultrasonic wave
The supersonic frequency of reason is 950kHz, while nitrogen is continually fed into the slurry, and the flow of nitrogen is 1.5m/s, obtains one
Secondary enzymolysis liquid；
The primary enzymolysis liquid obtained by the step 2) processing is placed in a container by step 3), to the primary enzymolysis
Carbon dioxide is continually fed into liquid, is passed through the time of carbon dioxide as 2h, the flow of carbon dioxide is 4m/s,
The container is placed in high-pressure pulse electric afterwards and is handled, in the high-pressure pulse electric processing procedure, continues to lead to
Enter carbon dioxide, the flow of carbon dioxide is 1m/s, after the high-pressure pulse electric processing terminates, is also continued to
Carbon dioxide is passed through, the flow of carbon dioxide keeps one with the flow in the high-pressure pulse electric processing procedure
It causes, the electric field strength of the high-pressure pulse electric is 56kV/cm, and frequency 120Hz, pulse width is 22 μ s, and processing time is
8s；
The feed liquid that the step 3) processing obtains is placed 30min by step 4), is added neutral proteinase and is carried out secondary enzyme
Solution, secondary enzymolysis temperature are 43 DEG C, and pH value 8, the addition of the neutral proteinase handles obtained material for the step 3)
The 2% of liquid quality when the secondary enzymolysis time is 3 small, during secondary enzymolysis, is carried out at the same time ultrasonication, the ultrasound
The supersonic frequency of ripple processing is 650kHz, while nitrogen is continually fed into the slurry, and the flow of nitrogen is 4m/s, is obtained
Secondary enzymolysis liquid；Wherein, the neutral proteinase is by trypsase, papain and subtilopeptidase A, the pancreas
The mass ratio of protease, papain and subtilopeptidase A is 5:2:0.5；
Step 5) adds in the hydrochloric acid that mass concentration is 3% into the secondary enzymolysis liquid, by the pH of the secondary enzymolysis liquid
It adjusts to 4-5, continuously adds absolute ethyl alcohol, addition of the absolute ethyl alcohol in the secondary enzymolysis liquid is the secondary enzyme
The 70% of liquid product is solved, when standing 23 is small；
The feed liquid that the step 5) is prepared is placed in centrifugation apparatus and is centrifuged by step 6), and centrifuge RPMs are
8500r/min, the time of centrifugal treating is 26min；
Step 7) is handled in obtained fish oil with the step 6) and adds in activated carbon, and the addition of the activated carbon is described
The 3% of the fish oil quality that step 6) processing obtains；Again using centrifugation apparatus by Activated carbon separation, so as to obtain fish oil.
Embodiment 4
Step 1) is cleaned up tuna leftover bits and pieces with clear water, is smashed, and addition is equivalent to 2 times of the tuna leftover bits and pieces
Water be mixed into slurry, the slurry is stirred evenly；
Step 2) adds in pepsin into the slurry that the step 1) is prepared and carries out primary enzymolysis, primary enzymolysis
Temperature is 35 DEG C, pH value 3, when the primary enzymolysis time is 10 small, wherein, the addition of the pepsin is the step 1)
The 2.5% of the slurry quality being prepared during primary enzymolysis, is carried out at the same time ultrasonication, at the ultrasonic wave
The supersonic frequency of reason is 900kHz, while nitrogen is continually fed into the slurry, and the flow of nitrogen is 2m/s, is obtained once
Enzymolysis liquid；
The primary enzymolysis liquid obtained by the step 2) processing is placed in a container by step 3), to the primary enzymolysis
Carbon dioxide is continually fed into liquid, is passed through the time of carbon dioxide as 1h, the flow of carbon dioxide is 4m/s,
The container is placed in high-pressure pulse electric afterwards and is handled, in the high-pressure pulse electric processing procedure, continues to lead to
Enter carbon dioxide, the flow of carbon dioxide is 1m/s, after the high-pressure pulse electric processing terminates, is also continued to
Carbon dioxide is passed through, the flow of carbon dioxide keeps one with the flow in the high-pressure pulse electric processing procedure
It causes, the electric field strength of the high-pressure pulse electric is 55kV/cm, and frequency 130Hz, pulse width is 20 μ s, and processing time is
8s；
The feed liquid that the step 3) processing obtains is placed 40min by step 4), is added neutral proteinase and is carried out secondary enzyme
Solution, secondary enzymolysis temperature are 43 DEG C, and pH value 8, the addition of the neutral proteinase handles obtained material for the step 3)
The 2% of liquid quality when the secondary enzymolysis time is 3 small, during secondary enzymolysis, is carried out at the same time ultrasonication, the ultrasound
The supersonic frequency of ripple processing is 680kHz, while nitrogen is continually fed into the slurry, and the flow of nitrogen is 3.5m/s, is obtained
To secondary enzymolysis liquid；Wherein, the neutral proteinase be by trypsase, papain and subtilopeptidase A, it is described
The mass ratio of trypsase, papain and subtilopeptidase A is 4:1:1；
Step 5) adds in the hydrochloric acid that mass concentration is 3% into the secondary enzymolysis liquid, by the pH of the secondary enzymolysis liquid
It adjusts to 4-5, continuously adds absolute ethyl alcohol, addition of the absolute ethyl alcohol in the secondary enzymolysis liquid is the secondary enzyme
The 70% of liquid product is solved, when standing 23 is small；
The feed liquid that the step 5) is prepared is placed in centrifugation apparatus and is centrifuged by step 6), and centrifuge RPMs are
9000r/min, the time of centrifugal treating is 33min；
Step 7) is handled in obtained fish oil with the step 6) and adds in activated carbon, and the addition of the activated carbon is described
The 4% of the fish oil quality that step 6) processing obtains；Again using centrifugation apparatus by Activated carbon separation, so as to obtain fish oil.
Embodiment 5
Step 1) is cleaned up tuna leftover bits and pieces with clear water, is smashed, and addition is equivalent to 2 times of the tuna leftover bits and pieces
Water be mixed into slurry, the slurry is stirred evenly；
Step 2) adds in pepsin into the slurry that the step 1) is prepared and carries out primary enzymolysis, primary enzymolysis
Temperature is 35 DEG C, pH value 3, when the primary enzymolysis time is 10 small, wherein, the addition of the pepsin is the step 1)
The 2.5% of the slurry quality being prepared during primary enzymolysis, is carried out at the same time ultrasonication, at the ultrasonic wave
The supersonic frequency of reason is 870kHz, while nitrogen is continually fed into the slurry, and the flow of nitrogen is 2.5m/s, obtains one
Secondary enzymolysis liquid；
The primary enzymolysis liquid obtained by the step 2) processing is placed in a container by step 3), to the primary enzymolysis
Carbon dioxide is continually fed into liquid, is passed through the time of carbon dioxide as 2h, the flow of carbon dioxide is 4.5m/
The container is placed in high-pressure pulse electric handles afterwards by s, in the high-pressure pulse electric processing procedure, continues
Be passed through carbon dioxide, the flow of carbon dioxide is 1m/s, after the high-pressure pulse electric processing terminates, also after
Continuous to be passed through carbon dioxide, the flow of carbon dioxide keeps one with the flow in the high-pressure pulse electric processing procedure
It causes, the electric field strength of the high-pressure pulse electric is 55kV/cm, and frequency 100Hz, pulse width is 20 μ s, and processing time is
5s；
The feed liquid that the step 3) processing obtains is placed 30-60min by step 4), and it is secondary to add neutral proteinase progress
Enzymolysis, secondary enzymolysis temperature are 45 DEG C, pH value 9, and the addition step 3) processing of the neutral proteinase obtains
The 3% of feed liquid quality during secondary enzymolysis, is carried out at the same time ultrasonication, and the supersonic frequency of the ultrasonication is
700kHz when the secondary enzymolysis time is 4 small, while is continually fed into nitrogen into the slurry, and the flow of nitrogen is 3.5m/s,
Obtain secondary enzymolysis liquid；Wherein, the neutral proteinase is by trypsase, papain and subtilopeptidase A, institute
The mass ratio for stating trypsase, papain and subtilopeptidase A is 5:2:1；
Step 5) adds in the hydrochloric acid that mass concentration is 3% into the secondary enzymolysis liquid, by the pH of the secondary enzymolysis liquid
It adjusts to 4-5, continuously adds absolute ethyl alcohol, addition of the absolute ethyl alcohol in the secondary enzymolysis liquid is the secondary enzyme
The 70% of liquid product is solved, when standing 23 is small；
The feed liquid that the step 5) is prepared is placed in centrifugation apparatus and is centrifuged by step 6), and centrifuge RPMs are
8800r/min, the time of centrifugal treating is 26min；
Step 7) is handled in obtained fish oil with the step 6) and adds in activated carbon, and the addition of the activated carbon is described
The 3% of the fish oil quality that step 6) processing obtains；Again using centrifugation apparatus by Activated carbon separation, so as to obtain fish oil.
Compliance test result：
Tuna oil is extracted using the method for embodiment 1 to embodiment 5.Wherein, each embodiment processing
1000g tuna leftover bits and pieces.The index of embodiment 1 to the fish oil that embodiment 5 is extracted is shown in Table 1.The present invention is improved to golden rifle
The recovery rate of fish fish oil improves the content of EPA and DHA in fish oil.
Table 1
Although the embodiments of the present invention have been disclosed as above, but its be not restricted in specification and embodiment it is listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, it is of the invention and unlimited
In specific details.
Claims (6)
1. a kind of recovery processing technique of tuna leftover bits and pieces, which is characterized in that including：
Step 1) is cleaned up tuna leftover bits and pieces with clear water, is smashed, and addition is equivalent to 0.5-2 times of the tuna leftover bits and pieces
Water be mixed into slurry, the slurry is stirred evenly；
Step 2) adds in pepsin into the slurry that the step 1) is prepared and carries out primary enzymolysis, primary enzymolysis temperature
For 30-35 DEG C, pH value 2-3, when the primary enzymolysis time is 9-10 small, wherein, the addition of the pepsin is the step
The 2-2.5% of the rapid slurry quality 1) being prepared, during primary enzymolysis, while is continually fed into the slurry
Nitrogen, the flow of nitrogen obtain primary enzymolysis liquid for 1.5-2.5m/s；
The primary enzymolysis liquid obtained by the step 2) processing is placed in a container by step 3), into the primary enzymolysis liquid
Carbon dioxide is continually fed into, is passed through the time of carbon dioxide as 1-2h, the flow of carbon dioxide is 4-5m/s,
The container is placed in high-pressure pulse electric afterwards and is handled, in the high-pressure pulse electric processing procedure, continues to lead to
Enter carbon dioxide, the flow of carbon dioxide is 1-2m/s, after the high-pressure pulse electric processing terminates, also after
Continuous to be passed through carbon dioxide, the flow of carbon dioxide keeps one with the flow in the high-pressure pulse electric processing procedure
It causing, the electric field strength of the high-pressure pulse electric is 55-60kV/cm, and frequency 100-150Hz, pulse width is 20-25 μ s,
Processing time is 5-10s；
The feed liquid that the step 3) processing obtains is placed 30-60min by step 4), is added neutral proteinase and is carried out secondary enzyme
Solution, secondary enzymolysis temperature are 40-45 DEG C, pH value 8-9, and the addition of the neutral proteinase obtains for the step 3) processing
Feed liquid quality 2-3%, the secondary enzymolysis time for 3-4 it is small when, during secondary enzymolysis, while held into the slurry
Continuous to be passed through nitrogen, the flow of nitrogen is 3.5-5.5m/s, obtains secondary enzymolysis liquid；Wherein, the neutral proteinase is by pancreas egg
White enzyme, papain and subtilopeptidase A, the quality of the trypsase, papain and subtilopeptidase A
Than for 4-5:1-2:0.5-1；
Step 5) adds in the hydrochloric acid that mass concentration is 2-3% into the secondary enzymolysis liquid, by the pH tune of the secondary enzymolysis liquid
Section continuously adds absolute ethyl alcohol, addition of the absolute ethyl alcohol in the secondary enzymolysis liquid is the secondary enzymolysis to 4-5
The 65-70% of liquid product, when standing 20-23 is small；
The feed liquid that the step 5) is prepared is placed in centrifugation apparatus and is centrifuged by step 6), and centrifuge RPMs are
8000-9000r/min, the time of centrifugal treating is 26-33min.
2. the recovery processing technique of tuna leftover bits and pieces as described in claim 1, which is characterized in that in the step 2),
During primary enzymolysis, ultrasonication is carried out at the same time, the supersonic frequency of the ultrasonication is 800-950kHz.
3. the recovery processing technique of tuna leftover bits and pieces as claimed in claim 2, which is characterized in that in the step 2),
During primary enzymolysis, the supersonic frequency of the ultrasonication is 800kHz.
4. the recovery processing technique of tuna leftover bits and pieces as described in claim 1, which is characterized in that in the step 4),
During secondary enzymolysis, ultrasonication is carried out at the same time, the supersonic frequency of the ultrasonication is 650-700kHz.
5. the recovery processing technique of tuna leftover bits and pieces as claimed in claim 4, which is characterized in that in the step 4), institute
The supersonic frequency for stating ultrasonication is 700kHz.
6. the recovery processing technique of tuna leftover bits and pieces as described in claim 1, which is characterized in that in the step 3), to
Carbon dioxide is continually fed into the primary enzymolysis liquid, is passed through the time of carbon dioxide as 1h, carbon dioxide
Flow for 4m/s, in the high-pressure pulse electric processing procedure, continue to be passed through carbon dioxide, carbon dioxide
Flow is 1m/s.
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| CN112471479A (en) * | 2020-11-30 | 2021-03-12 | 青岛科技大学 | Seasoning base material prepared by taking fish cooking liquor as raw material and preparation method thereof |
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