CN107699334A - A kind of method that ultrasonic wave added aqueous enzymatic method extracts Squid Oil from sleeve-fish-processing waste - Google Patents
A kind of method that ultrasonic wave added aqueous enzymatic method extracts Squid Oil from sleeve-fish-processing waste Download PDFInfo
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- CN107699334A CN107699334A CN201710867372.8A CN201710867372A CN107699334A CN 107699334 A CN107699334 A CN 107699334A CN 201710867372 A CN201710867372 A CN 201710867372A CN 107699334 A CN107699334 A CN 107699334A
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- fish
- sleeve
- squid
- processing waste
- ultrasonic wave
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Classifications
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/025—Pretreatment by enzymes or microorganisms, living or dead
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
- C11B1/106—Production of fats or fatty oils from raw materials by extracting using ultra-sounds
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B13/00—Recovery of fats, fatty oils or fatty acids from waste materials
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/50—Reuse, recycling or recovery technologies
- Y02W30/74—Recovery of fats, fatty oils, fatty acids or other fatty substances, e.g. lanolin or waxes
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Meat, Egg Or Seafood Products (AREA)
Abstract
A kind of method that ultrasonic wave added aqueous enzymatic method extracts Squid Oil from sleeve-fish-processing waste, described method comprise the following steps：（1）It is homogenized after sleeve-fish-processing waste is cleaned；（2）After homogenate material is mixed with water, regulation pH is 2.5 3.0, and 10min is incubated in 30 DEG C of ultrasonic cleaners, is incorporated as the pepsin of even stock quality 0.1%, and constant temperature digests enzyme deactivation after 1.5h in ultrasonic cleaner, obtains enzymolysis liquid；（3）After enzymolysis liquid is cooled to room temperature, centrifuging and taking supernatant, and in supernatant add 2 times of volumes absolute ethyl alcohol, after 1 h at the uniform velocity being stirred on magnetic stirring apparatus, 2 h are stood at room temperature, supernatant liquor is transferred in another clean container, and rotary evaporation obtains Squid Oil after removing absolute ethyl alcohol therein；The present invention utilizes the biological enzymolysis technology of ultrasonic wave added, with reference to alcohol steep, can effectively improve recovery rate, and does not produce secondary pollution, is effectively increased the economic value of squid.
Description
Technical field
The present invention relates to a kind of method that ultrasonic wave added aqueous enzymatic method extracts Squid Oil from sleeve-fish-processing waste, category
In the processing extractive technique field of marine product.
Background technology
Polyunsaturated fatty acid is rich in deep sea fish oil（PUFA）, wherein main component is eicosapentaenoic acid（EPA）With
Docosahexaenoic acid（DHA）.Polyunsaturated fatty acid has a variety of physiologically actives, such as strengthens nervous function, intelligence development is good for
Brain, anticancer etc..Therefore marine products fish oil is big as the demand of medicine, health products and feed addictive, and the extraction of marine products fish oil is opened
Hair has become the focus of food resource utilization.
Squid has abundant nutritive value and good organoleptic feature, is a kind of worldwide welcome marine products
Product.In squid is processed, the processing waste such as calamary head, internal organ, skin accounts for 30% or so of squid quality, and some are processed into small
Pack based on leisure food and fish meal, fish oil etc., also have part to be even taken as discarded object arbitrarily to abandon or bury, both polluted
Environment, again without fully exploitation squid added value.It is the good of extraction fish oil containing abundant fat in squid processing fent
Raw material.Traditional fish oil extracting method mainly has cooking process, light alkali hydrolysis method etc., and wherein cooking process easily causes the broken of fish oil composition
Bad, although light alkali hydrolysis method recovery rate is high, sodium salt content is high in caused waste liquid.
The content of the invention
It is an object of the invention to overcome the shortcomings of the prior art, and a kind of ultrasonic wave added aqueous enzymatic method is provided from squid
The method that Squid Oil is extracted in processing waste, it utilizes the biological enzymolysis technology of ultrasonic wave added, can be effective with reference to alcohol steep
Recovery rate is improved, and does not produce secondary pollution, the fish oil of extraction is food-grade, will greatly improve the application value of squid, effectively
Increase the economic value of squid.
The purpose of the present invention is completed by following technical solution, and a kind of ultrasonic wave added aqueous enzymatic method is useless from squid processing
The method that Squid Oil is extracted in gurry, described method comprise the following steps：
（1）It is homogenized after sleeve-fish-processing waste is cleaned；
（2）After homogenate material is mixed with water, regulation pH is 2.5-3.0, and 10- is incubated in 30-40 DEG C of ultrasonic cleaner
20min, even stock quality 0.1-0.3% pepsin being incorporated as, constant temperature digests enzyme deactivation after 1.5-2h in ultrasonic cleaner,
Obtain enzymolysis liquid；It is adopted as pepsin to be digested, effect is good, and easy to operation, process stabilizing, saves, be efficient, green ring
Protect；Using ultrasonic assistant, be advantageous to improve enzymolysis efficiency, improve the recovery rate of Squid Oil；
Protease species in the present invention are crucial, and inventor has found only to be digested using pepsin, finally obtains
Squid Oil just has preferable color and organoleptic feature；
（3）After enzymolysis liquid is cooled to room temperature, centrifuging and taking supernatant, and in supernatant add 2-3 times of volume absolute ethyl alcohol,
After at the uniform velocity stirring 1-2 h on magnetic stirring apparatus, 2-4 h are stood at room temperature, supernatant liquor is transferred in another clean container, revolve
Squid Oil is obtained after turning evaporative removal absolute ethyl alcohol therein；
As preferred：Described step（1）In, sleeve-fish-processing waste refers to squid skin, internal organ, head, processing meat mincing etc., does not include
Ink sac
Described step（2）In, homogenate material and water in mass ratio 1：3-5 is mixed；The enzyme activity of the pepsin is 10000-
15000 U/g；
Described step（3）In, described Extraction solvent ethanol is food-grade, green non-poisonous, is easy to the application of follow-up Squid Oil；
The technological parameter of the centrifugation is：10-15min is centrifuged under the conditions of 6000-10000 rpm/min.
The present invention is digested using squid processing fent as raw material using neutral proteinase ultrasonic wave added constant temperature, normal temperature centrifugation,
Supernatant and precipitation branch are extracted through absolute ethyl alcohol, and stratification, supernatant liquor is transferred in another clean container at room temperature,
Rotary evaporation obtains Squid Oil after removing absolute ethyl alcohol therein；
It is adopted as neutral proteinase to be digested, effect is good, and easy to operation, process stabilizing, saves, be efficient, green；Adopt
With ultrasonic assistant, be advantageous to improve enzymolysis efficiency, improve the recovery rate of Squid Oil.
Protease species in the present invention are crucial, and inventor has found only to be digested using neutral proteinase, finally
The Squid Oil of acquisition just has preferable color and organoleptic feature.
Embodiment
Below in conjunction with specific embodiment, the present invention will be described in detail：A kind of ultrasonic wave added water enzyme of the present invention
The method that method extracts Squid Oil from sleeve-fish-processing waste, this method comprise the following steps：
（1）It is homogenized after sleeve-fish-processing waste is cleaned, wherein sleeve-fish-processing waste refers to squid skin, internal organ, head, processing meat mincing
Deng not including ink sac；
（2）After homogenate material is mixed with water, regulation pH is 2.5-3.0, and 10- is incubated in 30-40 DEG C of ultrasonic cleaner
20min, even stock quality 0.1-0.3% pepsin being incorporated as, constant temperature digests enzyme deactivation after 1.5-2h in ultrasonic cleaner,
Obtain enzymolysis liquid；It is adopted as pepsin to be digested, effect is good, and easy to operation, process stabilizing, saves, be efficient, green ring
Protect；Using ultrasonic assistant, be advantageous to improve enzymolysis efficiency, improve the recovery rate of Squid Oil；Protease species in the present invention
Crucial, inventor has found only to be digested using pepsin, the Squid Oil finally obtained just have preferable color with
Organoleptic feature.
（3）After enzymolysis liquid is cooled to room temperature, centrifuging and taking supernatant, and the anhydrous of 2-3 times of volume is added in supernatant
Ethanol, 2-4 h are stood at room temperature after at the uniform velocity stirring 1-2 h on magnetic stirring apparatus, supernatant liquor is transferred to another clean container
Interior, rotary evaporation obtains Squid Oil after removing absolute ethyl alcohol therein；Extraction solvent ethanol is food-grade, green non-poisonous, is easy to
The application of follow-up Squid Oil.
Described step（2）In, homogenate material and water in mass ratio 1：（3-5）Mixing；The enzyme activity of the pepsin is
10000-15000 U/g；Described step（3）In, the technological parameter of centrifugation is：Centrifuged under the conditions of 6000-10000 rpm/min
10-15min。
Claims (2)
1. a kind of method that ultrasonic wave added aqueous enzymatic method extracts Squid Oil from sleeve-fish-processing waste, it is characterised in that described side
Method comprises the following steps：
（1）It is homogenized after sleeve-fish-processing waste is cleaned；
（2）After homogenate material is mixed with water, regulation pH is 2.5-3.0, and 10- is incubated in 30-40 DEG C of ultrasonic cleaner
20min, even stock quality 0.1-0.3% pepsin being incorporated as, constant temperature digests enzyme deactivation after 1.5-2h in ultrasonic cleaner,
Obtain enzymolysis liquid；
（3）After enzymolysis liquid is cooled to room temperature, centrifuging and taking supernatant, and in supernatant add 2-3 times of volume absolute ethyl alcohol,
After at the uniform velocity stirring 1-2 h on magnetic stirring apparatus, 2-4 h are stood at room temperature, supernatant liquor is transferred in another clean container, revolve
Squid Oil is obtained after turning evaporative removal absolute ethyl alcohol therein.
2. the method that ultrasonic wave added aqueous enzymatic method according to claim 1 extracts Squid Oil from sleeve-fish-processing waste, its
It is characterised by：
Described step（1）In, the sleeve-fish-processing waste refers to squid skin, internal organ, head, processing meat mincing etc., not including ink sac；
Described step（2）In, homogenate material and water in mass ratio 1：3-5 is mixed；The enzyme activity of the pepsin is 10000-
15000 U/g；
Described step（3）In, described Extraction solvent ethanol is food-grade, is easy to the application of follow-up Squid Oil；The work of centrifugation
Skill parameter is：10-15min is centrifuged under the conditions of 6000-10000 rpm/min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710867372.8A CN107699334A (en) | 2017-09-22 | 2017-09-22 | A kind of method that ultrasonic wave added aqueous enzymatic method extracts Squid Oil from sleeve-fish-processing waste |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710867372.8A CN107699334A (en) | 2017-09-22 | 2017-09-22 | A kind of method that ultrasonic wave added aqueous enzymatic method extracts Squid Oil from sleeve-fish-processing waste |
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| CN107699334A true CN107699334A (en) | 2018-02-16 |
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| CN201710867372.8A Pending CN107699334A (en) | 2017-09-22 | 2017-09-22 | A kind of method that ultrasonic wave added aqueous enzymatic method extracts Squid Oil from sleeve-fish-processing waste |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108740839A (en) * | 2018-06-09 | 2018-11-06 | 浙江亿丰海洋生物制品有限公司 | A kind of method of squid leftover bits and pieces extraction nutrients |
Citations (6)
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|---|---|---|---|---|
| CN101940240A (en) * | 2010-07-01 | 2011-01-12 | 大连工业大学 | Method for preparing fish oil ethyl ester microcapsule from fish pomace |
| RU2468072C1 (en) * | 2011-06-29 | 2012-11-27 | Федеральное государственное унитарное предприятие "Всероссийский научно-исследовательский институт рыбного хозяйства и океанографии" (ФГУП "ВНИРО") | Fish liver oil production method |
| CN103540394A (en) * | 2013-08-27 | 2014-01-29 | 江苏科技大学 | Method for extracting silkworm pupa oil by utilizing ultrasonic assisted aqueous enzymatic method |
| CN103614235A (en) * | 2013-11-23 | 2014-03-05 | 威海市宇王集团海洋生物工程有限公司 | Method for extracting fish oil from fish heads and fish skin |
| CN102559369B (en) * | 2010-12-14 | 2014-05-07 | 大连工业大学 | Preparation method of antarctic krill oil |
| CN106819955A (en) * | 2017-01-23 | 2017-06-13 | 石狮市华宝海洋生物医药有限公司 | A kind of preparation technology of the stable type fish oil of decoloration deodorization |
-
2017
- 2017-09-22 CN CN201710867372.8A patent/CN107699334A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101940240A (en) * | 2010-07-01 | 2011-01-12 | 大连工业大学 | Method for preparing fish oil ethyl ester microcapsule from fish pomace |
| CN102559369B (en) * | 2010-12-14 | 2014-05-07 | 大连工业大学 | Preparation method of antarctic krill oil |
| RU2468072C1 (en) * | 2011-06-29 | 2012-11-27 | Федеральное государственное унитарное предприятие "Всероссийский научно-исследовательский институт рыбного хозяйства и океанографии" (ФГУП "ВНИРО") | Fish liver oil production method |
| CN103540394A (en) * | 2013-08-27 | 2014-01-29 | 江苏科技大学 | Method for extracting silkworm pupa oil by utilizing ultrasonic assisted aqueous enzymatic method |
| CN103614235A (en) * | 2013-11-23 | 2014-03-05 | 威海市宇王集团海洋生物工程有限公司 | Method for extracting fish oil from fish heads and fish skin |
| CN106819955A (en) * | 2017-01-23 | 2017-06-13 | 石狮市华宝海洋生物医药有限公司 | A kind of preparation technology of the stable type fish oil of decoloration deodorization |
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| 郭毛毛: "利用酶解法从泥鳅鱼和鳙鱼中提取混合鱼油的研究", 《农业与技术》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108740839A (en) * | 2018-06-09 | 2018-11-06 | 浙江亿丰海洋生物制品有限公司 | A kind of method of squid leftover bits and pieces extraction nutrients |
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