TW202214272A - Softshell turtle fat extract, extraction method and product containing extract thereof - Google Patents

Abstract

A softshell turtle fat extract is extracted from a softshell turtle foot part or a softshell turtle waste portion which contains monounsaturated fatty acids and polyunsaturated fatty acids. The softshell turtle fat extract has functions of physiological activities, including blood pressure regulation, anti-memory decline, anti-inflammatory, anti-aging and anti-oxidation. The softshell turtle fat extract is a non-cytotoxic material.

Description

Soft-shelled turtle fat extract, its method and its additive product

The present invention relates to a softshell turtle fat extract, its method and its added products; in particular, to a fat extract of softshell turtle limbs (or other discarded parts), its method and its added products.

For conventional soft-shelled turtle-related extract products, for example: the invention patent application of Republic of China Patent Publication No. TW-200900127 "Cosolvent Modified Supercritical Carbon Dioxide Removal of Cholesterol from Turtle Egg Powder and Extraction of Free Amino Acids", which A procedure method for removing cholesterol from turtle roe powder and extracting free amino acids by co-solvent modified supercritical carbon dioxide is disclosed. The procedure for extracting free amino acids employs a co-solvent selected from a polar substance such as water, acetone, methanol or ethanol.

Continuing from the above, the program method for extracting free amino acids in the aforementioned patent publication No. TW-200900127 comprises: pre-calculating the required ratio of co-solvent to carbon dioxide; then mixing the co-solvent with a turtle roe powder uniformly to obtain a soft-shelled turtle roe powder mixture; and then filling the soft-shelled turtle roe powder mixture into an extraction tank for extraction.

Continuing from the above, the program method for extracting free amino acids in the aforementioned patent publication No. TW-200900127 includes: a continuous addition method outside the tank, which pre-calculates the ratio of the co-solvent and carbon dioxide to be added, and calculates the ratio of the co-solvent and carbon dioxide to be added outside the tank according to the method. Add to the extraction tank for extraction at equal time intervals; or, a comprehensive pre- and continuous addition method outside the tank, which pre-calculates the required addition The ratio of this co-solvent to carbon dioxide, with the soft-shelled turtle roe powder, the co-solvent is pre-added in the extraction tank to wet and bulk the soft-shelled turtle roe powder, and the remaining co-solvent is mixed with carbon dioxide when the extraction starts. The conveying is added to the extraction tank for extraction to obtain a soft-shelled turtle roe powder extract.

In fact, the procedure for extracting free amino acids in the aforementioned Patent Publication No. TW-200900127 is obviously only suitable for extracting soft-shelled turtle roe powder extract or its related extracts, but it is not suitable for direct extraction or extraction and processing of soft-shelled turtle fat. Extract [extract] or fat extract [extract] from the limbs of soft-shelled turtle.

Another conventional soft-shelled turtle-related extract product, such as the invention patent application of the Republic of China Patent Publication No. TW-201119593 “Processing Method and Finished Product of Turtle Embryosin”, discloses a processing method and finished product of Turtle Embryosin. The processing method of the turtle embryonic element comprises: selecting a good turtle embryo.

Continuing from the above, the processing method of the turtle embryonic element of the aforementioned patent publication No. TW-201119593 comprises: incubating a fertilized embryo by a natural method, selecting a vigorous turtle egg embryo material, and then using the turtle egg embryo. The material is washed, dried and sterilized in wine, and then exposed to a sunlight ultraviolet ray to remove the fishy smell and moisture of the turtle egg embryo material, and then add a strain of wine to ferment, so that the organic matter is converted into a polysaccharide, and then the The turtle egg embryo material is dried at high temperature and ground to form a powder, and the powder is made into a capsule or a tablet.

In fact, the processing method of the turtle embryonic element in the aforementioned Patent Publication No. TW-201119593 is obviously only suitable for processing turtle egg embryo material powder or related embryo materials, but it is not suitable for direct extraction or extraction of turtle fat. extract [extract] or fat extract [extract] from the limbs of soft-shelled turtles.

Another customary manufacturing method of turtle essence, such as: Li published the invention patent application No. TW-200616558 "Manufacturing method of soft-shelled turtle essence", which discloses a manufacturing method of soft-shelled turtle essence. This soft-shelled turtle essence manufacturing method comprises: a. cleaning and sterilizing the fresh soft-shelled turtle; b. using a low temperature to freeze the fresh soft-shelled soft-shelled turtle into a hibernation state to obtain a hibernating state soft-shelled turtle; c. at a low temperature Dissect the soft-shelled turtle in the hibernation state, remove a viscera, and remove a fat to obtain a dissected soft-shelled turtle; d. Quickly freeze the dissected soft-shelled turtle to obtain a dissected frozen soft-shelled turtle.

Continuing from the above, the above-mentioned patent publication No. TW-200616558, this soft-shelled turtle essence manufacturing method further comprises: e. this dissected frozen soft-shelled turtle is thawed to a relatively high temperature from a frozen state to obtain a dissected and thawed soft-shelled turtle; f. The dissection and thawed soft-shelled turtle is heated and dried to obtain a dried soft-shelled turtle; g. the dried soft-shelled turtle is ground to obtain a soft-shelled turtle meal; h, the soft-shelled turtle powder is sterilized and vacuum-packed to obtain a soft-shelled turtle meal. A semi-finished turtle meal.

Continuing from the above, the above-mentioned patent publication No. TW-200616558, the soft-shelled turtle essence manufacturing method further comprises: i. After the fat taken out in step c is crushed, thermally concentrated, filtered out of oil residue, winterized, filled with nitrogen and other processes , obtain a semi-finished product of turtle oil; j, finally, mix and stir the semi-finished product of turtle oil and semi-finished turtle meal to obtain a mixture of semi-finished product of turtle oil and semi-finished turtle meal, and package the semi-finished product of turtle oil and semi-finished turtle powder with a soft capsule mixture to form a Turtle Essence product.

In fact, the above-mentioned patent publication No. TW-200616558 is obviously only applicable to the processing of semi-finished products of soft-shelled turtle oil and semi-finished products of soft-shelled turtle powder or the related products of soft-shelled turtle essence, but it is not applicable to the direct extraction or extraction of soft-shelled turtles Fat extract [extract] or fat extract [extract] from the limbs of soft-shelled turtle.

Another conventional technology for the concentration and treatment of turtle polyunsaturated fatty acid ethyl ester oil, such as the invention patent of the Republic of China Patent Publication No. TW-200904967 "The Concentration Method of Turtle Polyunsaturated Fatty Acid Ethyl Ester Oil" Application, which discloses a method for concentrating turtle polyunsaturated fatty acid ethyl ester oil. The method for concentrating the turtle polyunsaturated fatty acid ethyl ester oil comprises: A. continuously feeding a turtle ethyl ester oil into an extraction tank, and using a supercritical carbon dioxide to carry out a turtle oil ethyl ester process at the bottom of the extraction tank with a supercritical carbon dioxide. extraction.

Continuing from the above, the concentration method of the turtle polyunsaturated fatty acid ethyl ester oil of the aforementioned patent publication No. TW-200904967 further comprises: B, the light component of the turtle oil ethyl ester [high solubility, such as C14 to 16] and part of the heavy component [Low solubility, such as C20 to 24] is dissolved by the supercritical carbon dioxide and collected in a separation tank; C. Part of the distillate in the separation tank is refluxed to the top of the extraction tank for continuous extraction and distillation; D. After the extraction had progressed and equilibrated, the distillate was collected, and a raffinate was collected.

In fact, the method for concentrating the soft-shelled turtle polyunsaturated fatty acid ethyl ester oil disclosed in the aforementioned Patent Publication No. TW-200904967 is obviously only applicable to supercritical carbon dioxide treatment of soft-shelled turtle polyunsaturated fatty acid ethyl ester oil concentrate or its related concentrates, but its It is not suitable for direct extraction or extraction processing of soft-shelled turtle fat extract (extract) or fat extract of soft-shelled turtle limbs (extract).

Obviously, the above-mentioned ROC Patent Publication No. TW-200900127 of the soft-shelled turtle roe powder extract, the soft-shelled turtle egg embryo material powder of No. TW-201119593, the semi-finished product of soft-shelled turtle oil and the semi-finished product of soft-shelled turtle No. There is still a certain need for further improvement of the No. 1 soft-shelled turtle polyunsaturated fatty acid ethyl ester oil concentrate.

Another conventional technique for extracting and processing turtle oil, such as the invention patent application of Chinese Patent Publication No. CN-1769409 "A Preparation Method of Refined Turtle Oil", discloses a preparation method of turtle oil. The preparation method of the soft-shelled turtle oil comprises: extracting the crude soft-shelled turtle oil at low temperature by extracting the soft-shelled soft-shelled turtle fat at a low temperature, and then performing degumming, decolorizing and dewaxing processes to obtain the refined soft-shelled turtle oil.

Continuing from the above, the preparation method of the turtle oil of the aforementioned patent publication No. CN-1769409 adopts: the supercritical fluid is carbon dioxide or propane; the temperature for selecting the supercritical fluid extraction is 30 ℃ to 120 ℃ and the pressure is 4MPa to 30MPa; or, The temperature of the supercritical fluid extraction is selected to be 40°C to 80°C and the pressure is 4MPa to 8MPa; or, the temperature of the supercritical fluid extraction is selected to be 40°C to 80°C and the pressure is 2MPa to 6MPa.

In fact, the preparation method of the turtle oil in the aforementioned Patent Publication No. CN-1769409 is obviously only suitable for supercritical fluid extraction and low-temperature extraction of turtle crude oil and its subsequent treatment process, but it is not suitable for direct extraction or extraction treatment of turtle fat. Extract [extract] or fat extract [extract] from the limbs of soft-shelled turtle.

Another conventional turtle oil extraction and processing technology, such as the invention patent application of Chinese Patent Publication No. CN-109580804 "Preparation Method and Detection Method of Turtle Oil with High Stability", discloses a preparation method of turtle oil. The preparation method of the turtle oil comprises the steps of: pulverizing, taking the fat of the turtle oil, fully pulverizing to obtain product A; freezing, placing product A in a mold box, freezing at -20° C. for 10 hours, and freezing into a plurality of small pieces, Obtain product B; for secondary pulverization, place product B in a grinder to fully pulverize to obtain product C; thaw naturally, thaw product C naturally to room temperature to obtain product D.

Continuing from the above, the preparation method of the turtle oil of the aforementioned patent publication No. CN-109580804 further comprises the steps of: adding enzyme, mixing D product into the neutral protease aqueous solution, stirring evenly, and obtaining E product; In a narrow-mouthed container, high-temperature nitrogen gas is introduced into product E, and the temperature of product E is heated to obtain product F.

Continuing from the above, the preparation method of the turtle oil of the aforementioned patent publication No. CN-109580804 further comprises steps: ultrasonic treatment, ultrasonic treatment is carried out to F product, and G product is obtained; centrifugal, centrifugal treatment is carried out to G product, and the centrifugal supernatant , to obtain H product; for preparation, add a mixture of fat-soluble tea polyphenols, vitamin E and fat-soluble rosemary extract to H product, and stir evenly.

In fact, the preparation method of the turtle oil in the aforementioned Patent Publication No. CN-109580804 is obviously only suitable for multiple pulverization, nitrogen heating and subsequent ultrasonic treatment, but it is not suitable for direct extraction or extraction treatment of turtle fat extract [extract] or fat extract [extract] from the limbs of soft-shelled turtles.

Obviously, the above-mentioned Chinese Patent Publication No. CN-1769409 adopts the supercritical fluid extraction of the preparation method of soft-shelled turtle oil and No. CN-109580804 adopts the multiple pulverization of the preparation method of soft-shelled turtle oil, nitrogen heating and the subsequent ultrasonic treatment program thereof are still all There must be a need for further improvement.

In short, the aforementioned ROC Patent Publication Nos. TW-200900127, TW-201119593, TW-200616558, TW-200904967, Chinese Patent Publication Nos. CN-1769409 and CN-109580804 patent applications It is only for reference to the technical background of the present invention and to describe the current state of the art, and it is not intended to limit the scope of the present invention.

In view of this, in order to meet the above-mentioned technical problems and demands, the present invention provides a soft-shelled turtle fat extract, a method thereof and an added product thereof, wherein the soft-shelled turtle fat extract is selected to be extracted from the fat of the limbs of a soft-shelled turtle or the fat of a discarded part of the soft-shelled turtle [ or other waste parts], and the soft-shelled turtle fat extract contains a monounsaturated fatty acid component and a polyunsaturated fatty acid component, and the soft-shelled turtle fat extract can regulate blood pressure, anti-memory decline, anti-inflammatory, anti-aging and anti-aging The application of physiological activities such as oxidation, and the soft-shelled turtle fat extract has no cytotoxicity, in order to expand the application and economic value of the discarded parts of conventional soft-shelled turtle or its fat extract.

The main purpose of the present invention is to provide a soft-shelled turtle fat extract, its method and its added products, wherein the soft-shelled turtle fat extract is selected from the fat of a soft-shelled turtle limb or a soft-shelled turtle waste fat [or other waste site], and the turtle fat extract contains a monounsaturated fatty acid component and a polyunsaturated fatty acid component, and the turtle fat extract can be used as blood pressure regulation, anti-memory decline, anti-inflammatory, anti-aging and anti-oxidation, etc. The application of physiological activity, and the soft-shelled turtle fat extract has no cytotoxicity, so as to achieve the purpose or effect of improving the application and economic value of the discarded parts of soft-shelled turtle or its fat extract.

In order to achieve the above-mentioned purpose, the extraction method of the soft-shelled turtle fat extract of the preferred embodiment of the present invention comprises:

Provide at least one fatty piece of soft-shelled turtle;

heating the soft-shelled turtle fat to a predetermined refining temperature to obtain a preliminary refined soft-shelled turtle oil;

cooling the preliminary refined turtle oil to obtain a cooled turtle oil;

centrifuging the cooled turtle oil at a predetermined centrifugal speed to obtain a separated turtle oil; and

The isolated turtle oil is filtered and purified to obtain a purified turtle oil, and the turtle fat extract is obtained from the purified turtle oil.

In a preferred embodiment of the present invention, the predetermined refining temperature ranges from 100°C to 200°C, 100°C to 300°C, 100°C to 400°C, 200°C to 300°C, and 200°C to 400°C between 300°C and 400°C.

In a preferred embodiment of the present invention, the soft-shelled turtle fat mass is selected from a soft-shelled turtle limb fat mass, a soft-shelled turtle limb near abdominal cavity fat mass, a soft-shelled turtle fat mass of discarded parts, or any combination thereof.

In a preferred embodiment of the present invention, the soft-shelled turtle fat extract is selected and extracted from the skirt part of a soft-shelled turtle.

In a preferred embodiment of the present invention, the preliminary refined turtle oil contains a turtle collagen component.

In order to achieve the above-mentioned purpose, the soft-shelled turtle fat extract of a preferred embodiment of the present invention comprises:

A soft-shelled turtle fat extract is selected to be extracted from the fat of a soft-shelled turtle limb part or a soft-shelled turtle waste part fat, and the soft-shelled turtle fat extract contains a monounsaturated fatty acid component and a polyunsaturated fatty acid component, and the soft-shelled turtle fat extract can be used as a The application of physiological activities such as regulating blood pressure, anti-memory decline, anti-inflammatory, anti-aging and anti-oxidation, and the soft-shelled turtle fat extract has no cytotoxicity.

The refining temperature of the soft-shelled turtle fat extract according to the preferred embodiment of the present invention is about 100°C.

The soft-shelled turtle fat extract of a preferred embodiment of the present invention further comprises a soft-shelled turtle collagen component.

In a preferred embodiment of the present invention, the soft-shelled turtle fat extract is selected and extracted from a fat mass of a soft-shelled turtle limb, a fat mass of a soft-shelled turtle limb near the abdominal cavity, a fat mass of a discarded part of a soft-shelled turtle, or any combination thereof.

In a preferred embodiment of the present invention, the soft-shelled turtle fat extract is selected and extracted from the skirt part of a soft-shelled turtle.

In order to achieve the above-mentioned purpose, the soft-shelled turtle fat extract added product of the preferred embodiment of the present invention comprises:

at least one cosmetic raw material having a first content; and

At least one soft-shelled turtle fat extract having a second content, and the soft-shelled turtle fat extract is extracted from a soft-shelled turtle fat block, and the soft-shelled turtle fat extract is added to the cosmetic raw material to prepare a cosmetic composition;

Wherein the soft-shelled turtle fat extract comprises a monounsaturated fatty acid component and a polyunsaturated fatty acid component, and the soft-shelled turtle fat extract can be used as physiological activities such as regulating blood pressure, anti-memory decline, anti-inflammatory, anti-aging and anti-oxidation, and The soft-shelled turtle fat extract was not cytotoxic.

The extraction of the soft-shelled turtle fat extract according to the preferred embodiment of the present invention The smelting temperature is about 100°C.

According to a preferred embodiment of the present invention, the soft-shelled turtle fat extract comprises a soft-shelled turtle collagen component.

In a preferred embodiment of the present invention, the cosmetic raw material is selected from a skin care product raw material, a color cosmetic product raw material or a perfume raw material, so as to make a skin care product, a color cosmetic product or a perfume.

In a preferred embodiment of the present invention, the cosmetic raw material is selected from a powder raw material, a paste raw material or a liquid raw material.

In order to achieve the above-mentioned purpose, the soft-shelled turtle fat extract added product of the preferred embodiment of the present invention comprises:

at least one food material having a first content; and

At least one turtle fat extract having a second content, and the turtle fat extract is extracted from a turtle fat block, and the turtle fat extract is added to the food material to prepare a food composition;

Wherein the soft-shelled turtle fat extract comprises a monounsaturated fatty acid component and a polyunsaturated fatty acid component, and the soft-shelled turtle fat extract can be used as physiological activities such as regulating blood pressure, anti-memory decline, anti-inflammatory, anti-aging and anti-oxidation, and The soft-shelled turtle fat extract was not cytotoxic.

The soft-shelled turtle fat extract of a preferred embodiment of the present invention further comprises a soft-shelled turtle collagen component.

In a preferred embodiment of the present invention, the food composition is a beverage, a food, a seasoning sauce, a seasoning powder, a health food or a health care product.

In a preferred embodiment of the present invention, the soft-shelled turtle fat extract is a soft-shelled turtle essence liquid, a soft-shelled soft-shelled turtle oil, a soft-shelled soft-shelled turtle essence tablet, a soft-shelled soft-shelled turtle essence granule, a soft-shelled soft-shelled turtle essence powder or any combination thereof.

1: Turtle fat block

10: Storage Carrier

10': storage carrier

10a: Mask

10b: Patch

10c: gauze body

10d: Bandage Roll

10e: Capsules

10f: packing bag

10g: sub-packaging box

10h: OK stretch

100: Processing equipment

11: First side

12: Second side

13: Configuration space

2: Cosmetic raw materials

20: Food ingredients

3: Turtle Fat Extract

a: cosmetic composition

b: food composition

A: Outer surface

B: inner surface

Figure 1: Addition of the soft-shelled turtle fat extract according to the first preferred embodiment of the present invention Block diagram of the product.

Figure 2: The structure schematic diagram of the soft-shelled turtle fat extract supplemented product according to the preferred embodiment of the present invention and its use of various storage carriers.

Figure 3: a schematic flow chart of the extraction method of the turtle fat extract according to the preferred embodiment of the present invention.

Figure 4: A block schematic diagram of the product added with the soft-shelled turtle fat extract according to the second preferred embodiment of the present invention.

Figure 4A: a schematic side view of the turtle oil transparent capsule selected for the soft-shelled turtle fat extract of another preferred embodiment of the present invention.

Figure 5: A schematic diagram of the yield of turtle oil (extraction rate) and the extraction temperature of the turtle fat extract according to the preferred embodiment of the present invention.

Figure 6: A schematic diagram of the test results of the DPPH free radical scavenging ability of the turtle fat extract according to the preferred embodiment of the present invention.

Figure 7: A schematic diagram of the test results of the cellular reactive oxygen species scavenging ability and cell viability of the turtle fat extract according to the preferred embodiment of the present invention.

Figure 8: A schematic diagram of the complete flow of the application of the turtle fat extract according to the preferred embodiment of the present invention to animal experiments.

Fig. 9: A schematic diagram of the evaluation results of the application of the soft-shelled turtle fat extract of the preferred embodiment of the present invention to the exploratory ability of aging rats.

Figure 10: A schematic diagram of the results of the evaluation of short-term spatial memory and motor performance in aging rats using the soft-shelled turtle fat extract according to the preferred embodiment of the present invention.

In order to fully understand the present invention, preferred embodiments will be exemplified below and described in detail in conjunction with the accompanying drawings, which are not intended to limit the present invention.

The soft-shelled turtle fat extract, its method and its added products according to the preferred embodiment of the present invention are suitable for various soft-shelled freshwater turtle species (also known as Chinese soft-shelled turtle, soft-shelled turtle, water fish, Tuanyu, mud turtle, or other soft-shelled turtle species). , and the soft-shelled turtle fat extract added product processing system and method of the preferred embodiment of the present invention can be selected to be applied in combination with various automatic equipment, various semi-automatic equipment, etc. Automatic equipment or various non-automatic equipment, but it is not used to limit the application scope of the present invention.

FIG. 1 discloses a block schematic diagram of the first preferred embodiment of the soft-shelled turtle fat extract-added product of the present invention. Please refer to FIG. 1 , for example, the soft-shelled turtle fat extract supplemented product according to the first preferred embodiment of the present invention adopts a storage carrier 10 or a device with similar functions, and the soft-shelled turtle fat extract supplemented product includes at least one Cosmetic raw material 2 and at least one soft-shelled turtle fat extract 3, and the cosmetic raw material 2 and soft-shelled turtle fat extract 3 are made into a cosmetic composition a.

Referring to FIG. 1 again, for example, the storage carrier 10 is used for storing and carrying at least one product combination, so that an appropriate usage amount of the product combination can be taken out from the storage carrier 10 during use. In addition, the storage carrier 10 can be made of various materials (such as plastic, aluminum tissue, glass, metal or other suitable materials), and the storage carrier 10 can also be made of a transparent material, semi-transparent material material or an opaque material.

FIG. 2 discloses the structure diagram of the soft-shelled turtle fat extract supplemented product according to the preferred embodiment of the present invention and its use of various storage carriers. Please refer to Figures 1 and 2, for example, the storage carrier 10 is selected from a facial mask 10a, a patch 10b, a gauze 10c, and a bandage 10d, one capsule (capsule) 10e (i.e. transparent capsule), one packing bag (packing bag) 10f, one packing box (packing box) 10g, one OK bandage (adhesive bandage) 10h or any combination thereof, and the The cloth 10b can be selected from an adhesive skin closure, a sterilization patch or a medical patch, and the storage carrier 10 can also be selected from a surgical tape or a It has a similar function of tape.

Please refer to Figures 1 and 2 again, for example, the storage The carrier 10 has a first side 11 and a second side 12 , and the storage carrier 10 includes an outer surface A and an inner surface B. The outer surface A is located on the first side 11 of the storage carrier 10 , and the inner surface B is located on the second side 12 of the storage carrier 10 . The first side 11 of the storage carrier 10 is an outer side, and the second side 12 of the storage carrier 10 is an inner side.

Referring again to Figures 1 and 2, for example, at least one dispensing space 13 is located on the inner surface B of the second side 12 of the storage carrier 10, and the disposing space 13 is used for accommodating the cosmetic The composition a or similar materials, and the configuration space 13 is selected from an airtight space, an unsealable airtight space, a semi-open space or any combination thereof.

Please refer to Figures 1 and 2 again, for example, the cosmetic raw material 2 has a first content, and the cosmetic raw material 2 is selected from a skin care product raw material, a color cosmetic raw material or a fragrance raw material to make a A skin care product, a color cosmetic product or a perfume, and the cosmetic raw material 2 is selected from a powder raw material, a cream or ointment raw material or a liquid raw material.

Referring again to Figures 1 and 2, for example, the turtle fat extract 3 is added to the cosmetic raw material 2 using a processing equipment 100 (or other equipment with similar functions), and the turtle fat extract 3 It can be selected to be extracted from the fat of the limbs of a soft-shelled turtle or the fat of a discarded part of the soft-shelled turtle, and the soft-shelled turtle fat extract 3 can be selected to be extracted from the fat mass of the limbs of a soft-shelled turtle, the fat mass of the limbs of the soft-shelled turtle near the abdominal cavity, the fat mass of the discarded part of the soft-shelled turtle, or any one of them. combination. In addition, the soft-shelled turtle fat extract 3 can also be selected to be extracted from the skirt part of a soft-shelled turtle or other parts.

Please refer to Figures 1 and 2 again, for example, the turtle fat extract 3 has a second content, and the turtle fat extract 3 includes a monounsaturated fatty acid component (eg: omega-9) and a Diversity is not Saturated fatty acid components [eg: omega-3, DHA, EPA], and the turtle fat extract 3 can be used for physiological activities such as regulating blood pressure, anti-memory decline, anti-inflammatory, anti-aging and anti-oxidation.

Please refer to Figures 1 and 2 again. For example, the turtle fat extract 3 is a turtle fat extract, a turtle fat extract, a turtle fat extract tablet, a turtle fat extract granule, a turtle fat extract, and a turtle fat extract. Turtle fat extract powder or any combination thereof.

Please refer to Figures 1 and 2, for example, the cosmetic raw material 2 and the soft-shelled turtle fat extract 3 or other soft-shelled turtle fat extract components [for example: soft-shelled turtle collagen components, including collagen blocks, collagen fragments or collagen powder] is arranged in the disposition space on the inner surface of the storage carrier 10 to make a cosmetic composition, and the storage carrier 10 can be selected from a cosmetic trial pack, a cosmetic bag, and a cosmetic box Or a cosmetic can.

FIG. 3 shows a schematic flow chart of the extraction method of the soft-shelled turtle fat extract according to the preferred embodiment of the present invention, which is compared with FIG. 1 . Referring to Figures 1 and 3, the method for extracting the soft-shelled turtle fat extract according to the preferred embodiment of the present invention includes step S1: first, for example, providing at least one Soft-shelled turtle fat mass 1 or a soft-shelled turtle fat material.

Referring again to Figures 1 and 3, the method for extracting the soft-shelled turtle fat extract according to the preferred embodiment of the present invention includes step S2: then, for example, by suitable technical means [for example: heating operation [electromagnetic heating device], The stirring operation (electromagnetic stirring device or other device) heats the soft-shelled turtle fat mass 1 to a predetermined refining temperature to obtain a preliminary refined soft-shelled turtle oil.

Please refer to Figures 1 and 3 again, for example, the extraction temperature of the soft-shelled turtle fat extract is about 100°C or other optimal extraction temperature in order to obtain; or, the predetermined extraction temperature is between 100°C and 200°C between, Between 100°C and 300°C, between 100°C and 400°C, between 200°C and 300°C, between 200°C and 400°C, or between 300°C and 400°C.

Referring again to Figures 1 and 3, the method for extracting the soft-shelled turtle fat extract according to a preferred embodiment of the present invention includes step S3: then, for example, using appropriate technical means (such as a heat sink or a cooling device) to extract the The preliminarily refined turtle oil is cooled for a predetermined period of time to obtain a cooled turtle oil.

Referring again to Figures 1 and 3, the method for extracting the soft-shelled turtle fat extract according to a preferred embodiment of the present invention includes step S4: then, for example, using appropriate technical means (such as a centrifugal device) to extract the cooled turtle The fish oil is centrifuged at a predetermined centrifugal speed for a predetermined time (for example: 10 minutes) to obtain a separated turtle oil.

Referring again to Figures 1 and 3, the method for extracting the soft-shelled turtle fat extract according to a preferred embodiment of the present invention includes step S5: then, for example, filtering and purifying the separated soft-shelled turtle oil by appropriate technical means , to obtain a purified turtle oil, and obtain the turtle fat extract 3 from the purified turtle oil.

Referring again to Figures 1 and 3, for example, the turtle fat extract 3 (or the extract) is appropriately subjected to freeze-drying operation (freeze-drying) with a freeze-drying device with appropriate technical means to obtain a Turtle fat extract powder, and the turtle fat extract powder contains a monounsaturated fatty acid component [eg: omega-9] and a polyunsaturated fatty acid component [eg: omega-3, DHA, EPA], and the turtle fat extract The powder can be used for physiological activities such as regulating blood pressure, anti-memory loss, anti-inflammatory, anti-aging and anti-oxidation.

Please refer to Figures 1 and 3. Generally speaking, nutritional studies have confirmed that the intake of unsaturated fatty acids is beneficial to cardiovascular function, and omega-3 polyunsaturated fatty acids directly affect the human heart, brain, eyes and nerves. The normal operation of the system, and has been confirmed in animal experiments to improve cognitive It has superior efficacy in the prevention of cancer and other related diseases derived from the aging process.

Table 1. Types and contents of fatty acids in the soft-shelled turtle fat extract (soft-shelled turtle oil)

As shown in Table 1, the unsaturated fatty acid of the turtle fat extract (soft turtle oil) according to the preferred embodiment of the present invention is more than about 70%, and the polyunsaturated fatty acid is about 22%.

FIG. 4 discloses a block schematic diagram of a product supplemented with a soft-shelled turtle fat extract according to a second preferred embodiment of the present invention. Referring to FIG. 4 , compared to the first embodiment, the product supplemented with the soft-shelled turtle fat extract according to the second preferred embodiment of the present invention includes at least one food material 20 and at least one soft-shelled turtle fat extract 3 , and the food material is 20 and the soft-shelled turtle fat extract 3 to prepare a food composition b.

Please refer to FIG. 4 again, for example, the food composition 20 is a beverage, a food, a seasoning sauce, a seasoning powder, a health food, a health care product or other products with similar compositions, and The storage carrier 10 can be selected from a product trial pack, a product bag, a product box or a product can.

Fig. 4A shows turtle fat according to another preferred embodiment of the present invention A schematic side view of a transparent capsule of turtle oil for fat extract selection. Referring to Fig. 4A, for example, a storage carrier 10' is selected from a transparent capsule, and the transparent capsule of the storage carrier 10' is used for accommodating the turtle fat extract 3 and other turtle fat extract components, such as : Soft-shelled turtle collagen composition [eg: collagen block, collagen fragment or collagen powder], and the soft-shelled turtle fat extract 3 is not cytotoxic.

Fig. 5 shows a schematic diagram of the yield of turtle oil (extraction rate) and the extraction temperature of the turtle fat extract according to the preferred embodiment of the present invention. Please refer to Figure 5. For example, the extraction method of the soft-shelled turtle fat extract according to the preferred embodiment of the present invention selects the extraction temperature as 100°C, 200°C, 300°C and 400°C, and the yield of the extraction temperature is 100°C. It is 30%, and its free radical scavenging ability is the best, and the yield of its refining temperature of 400 ℃ is 65%, and its yield increases with the refining temperature.

FIG. 6 is a schematic diagram showing the test results of the DPPH free radical scavenging ability of the turtle fat extract according to the preferred embodiment of the present invention. Please refer to Figure 6, for example, compared to the positive control substance of 0.6 mg/ml BHT, the turtle fat extract of the preferred embodiment of the present invention can remove more than 90% of free radicals, so the turtle fat extract [ turtle oil ] has significant antioxidant activity. Turtle oil extracted at a lower temperature (for example: the extraction temperature is about 100°C) has better free radical scavenging activity than that extracted at a higher temperature (for example: the extraction temperature is about 200°C, 300°C or 400°C), And its concentration is more than 25% to achieve superior effect.

FIG. 7 is a schematic diagram showing the test results of the cell reactive oxygen radical scavenging ability and the cell viability of the turtle fat extract according to the preferred embodiment of the present invention. Please refer to Figure 7. For example, at a non-cytotoxic concentration, the turtle fat extract (turtle oil) according to the preferred embodiment of the present invention can relieve the oxidative stress of cells and remove the active oxygen free radicals that cause aging. , and showed a dose-dependent response. As shown in Figure 7, asterisks represent statistically significant differences.

FIG. 8 discloses a schematic diagram of the complete flow of the application of the turtle fat extract according to the preferred embodiment of the present invention to animal experiments. Please refer to Figure 8. In this experiment, 40 8-week-old male rats of Sprague-Dawley (SD) strain were purchased from Lesco Biotechnology Co., Ltd. After the animals were purchased, they were kept at a constant temperature of 25±1°C Environment, 12-hour light cycle, acclimatized for one week before experimenting. The animals were equally divided into 5 groups according to body weight, with 8 animals in each group. The first group was not treated, the second group was subcutaneously injected with D-galatose at a dose of 120 mg/kg to accelerate the aging of rats, and the third group was fed after induction of aging. Turtle fat extract (soft-shell turtle oil), the fourth group was fed with turtle fat extract (soft-shell turtle oil) and involved in swimming training, and the fifth group was involved in swimming training after induction of aging. D-galatose was injected for a total of 42 days. The experimental samples and exercise training started 14 days after D-galatose injection and continued for 28 days. At the end of the experiment, Y-maze exploration and short-term spatial memory assessment, blood pressure and exercise performance weight-bearing swimming were performed. According to the recommended intake of fish oil of 2 grams per kilogram of human body per day by the Food and Drug Administration of the Ministry of Health and Welfare and the conversion factor between rats and humans, the feeding amount of the turtle fat extract sample (soft-shelled turtle oil sample) is 175 mg/kg/day, every morning at 10 Point, the samples were fed after 2 weeks of induced aging, and the samples were fed for a total of 4 weeks before experimental evaluation.

FIG. 9 is a schematic diagram showing the results of the evaluation of the exploration ability of the aged rat using the soft-shelled turtle fat extract according to the preferred embodiment of the present invention. Please refer to Figure 9. In this experiment, there was no difference in the average number of times of entering the new environment for the animals in each group; the time that the animals stayed in the new environment was recorded, and it was found that the second group of induced aging rats stayed in the new environment for a significant time. Shorter than the first group, the third group was fed with turtle fat extract [soft-shelled turtle oil], the fourth group was fed with soft-shelled turtle fat extract [soft-shelled turtle oil] combined with swimming training, and the fifth group was given swimming training, all showed that the rats were in the new environment. Stay longer, and the fourth group stays the longest. It can be seen that providing soft-shelled turtle fat extract [soft-shelled turtle oil] and swimming training may enhance the motivation of aging rats to explore new environments.

Fig. 10 discloses a preferred embodiment of the soft-shelled turtle fat extraction of the present invention Schematic diagram of the short-term spatial memory and motor performance evaluation results of the aged rats. Please refer to Figure 10. In this experiment, after feeding and routine swimming training, food was used as an incentive to evaluate whether the short-term spatial memory and motor performance of rats were improved. The second group of aged rats had a significantly longer time to find food than the first group of normal rats. The third group was fed with turtle fat extract (soft-shelled turtle oil), the fourth group was fed with soft-shelled turtle fat extract (soft-shelled turtle oil) and swimming training and The fifth group of aged rats who had undergone swimming training could find food faster, and the combination of feeding and exercise training could significantly improve spatial memory. The physical fitness of the rats in each group was evaluated by the performance of weight-bearing swimming. The results showed that the physical fitness of the aged rats was lower than that of the normal rats, and the time of weight-bearing swimming was reduced. Routinely trained aged rats had the longest swimming time under weight, which was more than 2 times longer than that of aged rats.

Table 2. Rat systolic and diastolic blood pressure

As shown in Table 2, in this experiment, the systolic blood pressure (SBP) and diastolic blood pressure (DBP) of the aged rats were significantly higher than those of the normal rats. ], or combined with exercise training, both systolic and diastolic blood pressure were positively regulated. Regarding systolic and diastolic blood pressure in rats, asterisks [*] in Table 2 represent statistically significant differences compared to aged rats.

The above experimental data are preliminary obtained under specific conditions. The results of further experiments are only for easy understanding or reference to the technical content of the present invention, and other related experiments are still needed. The experimental data and its results are not intended to limit the scope of the present invention.

The aforementioned preferred embodiment is only an example of the present invention and its technical features, and the technology of this embodiment can still be implemented in various substantially equivalent modifications and/or alternative ways; therefore, the scope of the right of the present invention is subject to the appended patent application The scope defined by the scope shall prevail. The copyright in this case is restricted to be used for the purposes of the ROC patent application.

1: Turtle fat block

10: Storage Carrier

100: Processing equipment

2: Cosmetic raw materials

3: Turtle Fat Extract

a: cosmetic composition

Claims (10)

A soft-shelled turtle fat extract comprising: A soft-shelled turtle fat extract is selected to be extracted from the fat of a soft-shelled turtle limb part or a soft-shelled turtle waste part fat, and the soft-shelled turtle fat extract contains a monounsaturated fatty acid component and a polyunsaturated fatty acid component, and the soft-shelled turtle fat extract can be used as a The application of physiological activities such as regulating blood pressure, anti-memory decline, anti-inflammatory, anti-aging and anti-oxidation, and the soft-shelled turtle fat extract has no cytotoxicity. The soft-shelled turtle fat extract according to claim 1, wherein the soft-shelled turtle fat extract comprises a soft-shelled turtle collagen component. The soft-shelled turtle fat extract according to claim 1, wherein the soft-shelled turtle fat extract is selected from a fat mass of a soft-shelled turtle limb, a fat mass of a soft-shelled turtle limb near the abdominal cavity, a fat mass of a discarded part of a soft-shelled turtle or any combination thereof. According to the soft-shelled turtle fat extract described in item 1 of the claimed scope, wherein the soft-shelled turtle fat extract is selected and extracted from the skirt part of a soft-shelled turtle. A soft-shelled turtle fat extract supplemented product comprising: at least one cosmetic raw material having a first content; and At least one turtle fat extract having a second content, and the turtle fat extract is extracted from a turtle fat block, and the turtle fat extract is added to the cosmetic raw material to make a cosmetic composition; Wherein the soft-shelled turtle fat extract comprises a monounsaturated fatty acid component and a polyunsaturated fatty acid component, and the soft-shelled turtle fat extract can be used for physiological activities such as regulating blood pressure, anti-memory decline, anti-inflammatory, anti-aging and anti-oxidation, and The turtle fat extract was not cytotoxic. A soft-shelled turtle fat extract supplemented product comprising: at least one food material having a first content; and At least one soft-shelled turtle fat extract having a second content, and the soft-shelled turtle fat extract is extracted from a soft-shelled turtle fat block, and the soft-shelled turtle fat extract is added to the food material to prepare a food composition; Wherein the soft-shelled turtle fat extract contains a monounsaturated fatty acid component And a polyunsaturated fatty acid component, and the soft-shelled turtle fat extract can regulate blood pressure, anti-memory decline, anti-inflammatory, anti-aging and anti-oxidation and other physiological activities, and the soft-shelled turtle fat extract has no cytotoxicity. According to the product supplemented with the soft-shelled turtle fat extract as described in claim 5 or 6, the refining temperature of the soft-shelled turtle fat extract in a preferred embodiment of the present invention is about 100°C. According to the product supplemented with the soft-shelled turtle fat extract described in the 5th or 6th application, the soft-shelled turtle fat extract is selected and extracted from a fat mass of a soft-shelled turtle limb, a fat mass of a soft-shelled turtle limb near the abdominal cavity, a fat mass of a discarded part of a soft-shelled turtle or any of them. combination. According to the product supplemented with the soft-shelled turtle fat extract described in claim 5 or 6, the soft-shelled turtle fat extract is selected and extracted from the skirt part of a soft-shelled turtle. According to the product supplemented with the soft-shelled turtle fat extract according to the claim 5 or 6, the soft-shelled turtle fat extract further comprises a soft-shelled turtle collagen component.

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