CN108095127A - Silybum marianum seed oil polypeptide mixture and preparation method thereof - Google Patents
Silybum marianum seed oil polypeptide mixture and preparation method thereof Download PDFInfo
- Publication number
- CN108095127A CN108095127A CN201711464474.1A CN201711464474A CN108095127A CN 108095127 A CN108095127 A CN 108095127A CN 201711464474 A CN201711464474 A CN 201711464474A CN 108095127 A CN108095127 A CN 108095127A
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- China
- Prior art keywords
- seed oil
- silybum marianum
- parts
- marianum seed
- enzymolysis
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Links
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 145
- 235000015112 vegetable and seed oil Nutrition 0.000 title claims abstract description 89
- 235000010841 Silybum marianum Nutrition 0.000 title claims abstract description 87
- 244000272459 Silybum marianum Species 0.000 title claims abstract description 85
- 239000000203 mixture Substances 0.000 title claims abstract description 61
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 57
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 50
- 238000002360 preparation method Methods 0.000 title claims abstract description 40
- 240000008042 Zea mays Species 0.000 claims abstract description 97
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 97
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 97
- 235000005822 corn Nutrition 0.000 claims abstract description 97
- 244000163122 Curcuma domestica Species 0.000 claims abstract description 65
- 235000003392 Curcuma domestica Nutrition 0.000 claims abstract description 65
- 235000003373 curcuma longa Nutrition 0.000 claims abstract description 65
- 235000013976 turmeric Nutrition 0.000 claims abstract description 65
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 28
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 claims abstract description 21
- 229920000168 Microcrystalline cellulose Polymers 0.000 claims abstract description 18
- 235000019813 microcrystalline cellulose Nutrition 0.000 claims abstract description 18
- 235000019359 magnesium stearate Nutrition 0.000 claims abstract description 14
- 239000000377 silicon dioxide Substances 0.000 claims abstract description 14
- 239000002994 raw material Substances 0.000 claims abstract description 11
- 239000008108 microcrystalline cellulose Substances 0.000 claims abstract description 10
- 229940016286 microcrystalline cellulose Drugs 0.000 claims abstract description 10
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 claims abstract description 9
- 235000013305 food Nutrition 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims description 52
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 20
- 102000004190 Enzymes Human genes 0.000 claims description 19
- 108090000790 Enzymes Proteins 0.000 claims description 19
- 229940088598 enzyme Drugs 0.000 claims description 19
- 239000003921 oil Substances 0.000 claims description 18
- 235000019198 oils Nutrition 0.000 claims description 18
- 239000000843 powder Substances 0.000 claims description 18
- 239000004365 Protease Substances 0.000 claims description 17
- 238000002156 mixing Methods 0.000 claims description 16
- 238000003756 stirring Methods 0.000 claims description 16
- 238000001914 filtration Methods 0.000 claims description 15
- 238000005469 granulation Methods 0.000 claims description 15
- 230000003179 granulation Effects 0.000 claims description 15
- 229920002494 Zein Polymers 0.000 claims description 12
- 239000005019 zein Substances 0.000 claims description 12
- 229940093612 zein Drugs 0.000 claims description 12
- 108091005804 Peptidases Proteins 0.000 claims description 10
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 10
- 239000003513 alkali Substances 0.000 claims description 10
- 239000000084 colloidal system Substances 0.000 claims description 10
- 230000009849 deactivation Effects 0.000 claims description 10
- 235000019419 proteases Nutrition 0.000 claims description 10
- 238000010521 absorption reaction Methods 0.000 claims description 9
- 239000011230 binding agent Substances 0.000 claims description 8
- 238000001035 drying Methods 0.000 claims description 8
- 108090000526 Papain Proteins 0.000 claims description 7
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 claims description 7
- 239000000796 flavoring agent Substances 0.000 claims description 7
- 235000019634 flavors Nutrition 0.000 claims description 7
- 235000019834 papain Nutrition 0.000 claims description 7
- 229940055729 papain Drugs 0.000 claims description 7
- 239000007864 aqueous solution Substances 0.000 claims description 6
- 239000000920 calcium hydroxide Substances 0.000 claims description 6
- 229910001861 calcium hydroxide Inorganic materials 0.000 claims description 6
- 238000010792 warming Methods 0.000 claims description 6
- 238000012216 screening Methods 0.000 claims description 5
- 239000000463 material Substances 0.000 claims 1
- 208000011580 syndromic disease Diseases 0.000 claims 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract description 11
- 231100000753 hepatic injury Toxicity 0.000 abstract description 10
- 230000001681 protective effect Effects 0.000 abstract description 5
- 230000002265 prevention Effects 0.000 abstract description 4
- 235000019441 ethanol Nutrition 0.000 description 9
- 210000004185 liver Anatomy 0.000 description 9
- 230000000694 effects Effects 0.000 description 8
- 210000005229 liver cell Anatomy 0.000 description 7
- VFLDPWHFBUODDF-FCXRPNKRSA-N curcumin Chemical compound C1=C(O)C(OC)=CC(\C=C\C(=O)CC(=O)\C=C\C=2C=C(OC)C(O)=CC=2)=C1 VFLDPWHFBUODDF-FCXRPNKRSA-N 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 150000001413 amino acids Chemical class 0.000 description 5
- 230000002255 enzymatic effect Effects 0.000 description 5
- 239000004615 ingredient Substances 0.000 description 5
- 210000002966 serum Anatomy 0.000 description 5
- 206010067125 Liver injury Diseases 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000010008 shearing Methods 0.000 description 4
- 241000699670 Mus sp. Species 0.000 description 3
- 235000012754 curcumin Nutrition 0.000 description 3
- 229940109262 curcumin Drugs 0.000 description 3
- 239000004148 curcumin Substances 0.000 description 3
- VFLDPWHFBUODDF-UHFFFAOYSA-N diferuloylmethane Natural products C1=C(O)C(OC)=CC(C=CC(=O)CC(=O)C=CC=2C=C(OC)C(O)=CC=2)=C1 VFLDPWHFBUODDF-UHFFFAOYSA-N 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- MBMQEIFVQACCCH-UHFFFAOYSA-N trans-Zearalenon Natural products O=C1OC(C)CCCC(=O)CCCC=CC2=CC(O)=CC(O)=C21 MBMQEIFVQACCCH-UHFFFAOYSA-N 0.000 description 3
- MBMQEIFVQACCCH-QBODLPLBSA-N zearalenone Chemical compound O=C1O[C@@H](C)CCCC(=O)CCC\C=C\C2=CC(O)=CC(O)=C21 MBMQEIFVQACCCH-QBODLPLBSA-N 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241000699666 Mus <mouse, genus> Species 0.000 description 2
- 241000320380 Silybum Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 235000019658 bitter taste Nutrition 0.000 description 2
- 238000007705 chemical test Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 208000002173 dizziness Diseases 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 238000003304 gavage Methods 0.000 description 2
- 230000002209 hydrophobic effect Effects 0.000 description 2
- 150000002561 ketenes Chemical class 0.000 description 2
- 230000002633 protecting effect Effects 0.000 description 2
- 150000003254 radicals Chemical class 0.000 description 2
- VZGDMQKNWNREIO-UHFFFAOYSA-N tetrachloromethane Chemical compound ClC(Cl)(Cl)Cl VZGDMQKNWNREIO-UHFFFAOYSA-N 0.000 description 2
- 231100000167 toxic agent Toxicity 0.000 description 2
- 239000003440 toxic substance Substances 0.000 description 2
- 231100000765 toxin Toxicity 0.000 description 2
- OYHQOLUKZRVURQ-NTGFUMLPSA-N (9Z,12Z)-9,10,12,13-tetratritiooctadeca-9,12-dienoic acid Chemical compound C(CCCCCCC\C(=C(/C\C(=C(/CCCCC)\[3H])\[3H])\[3H])\[3H])(=O)O OYHQOLUKZRVURQ-NTGFUMLPSA-N 0.000 description 1
- 206010000234 Abortion spontaneous Diseases 0.000 description 1
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 1
- 235000008495 Chrysanthemum leucanthemum Nutrition 0.000 description 1
- 244000192528 Chrysanthemum parthenium Species 0.000 description 1
- 235000000604 Chrysanthemum parthenium Nutrition 0.000 description 1
- CMSMOCZEIVJLDB-UHFFFAOYSA-N Cyclophosphamide Chemical compound ClCCN(CCCl)P1(=O)NCCCO1 CMSMOCZEIVJLDB-UHFFFAOYSA-N 0.000 description 1
- 206010013082 Discomfort Diseases 0.000 description 1
- 241000223195 Fusarium graminearum Species 0.000 description 1
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 1
- 241001529936 Murinae Species 0.000 description 1
- 102000015636 Oligopeptides Human genes 0.000 description 1
- 108010038807 Oligopeptides Proteins 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 241000234314 Zingiber Species 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- 230000002929 anti-fatigue Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- FIVPIPIDMRVLAY-UHFFFAOYSA-N aspergillin Natural products C1C2=CC=CC(O)C2N2C1(SS1)C(=O)N(C)C1(CO)C2=O FIVPIPIDMRVLAY-UHFFFAOYSA-N 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 238000010241 blood sampling Methods 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 229960004397 cyclophosphamide Drugs 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 230000001076 estrogenic effect Effects 0.000 description 1
- 235000008384 feverfew Nutrition 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- FIVPIPIDMRVLAY-RBJBARPLSA-N gliotoxin Chemical compound C1C2=CC=C[C@H](O)[C@H]2N2[C@]1(SS1)C(=O)N(C)[C@@]1(CO)C2=O FIVPIPIDMRVLAY-RBJBARPLSA-N 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 231100000234 hepatic damage Toxicity 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 125000001165 hydrophobic group Chemical group 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 208000000509 infertility Diseases 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 231100000535 infertility Toxicity 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 229960003284 iron Drugs 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 1
- 235000020778 linoleic acid Nutrition 0.000 description 1
- 230000008818 liver damage Effects 0.000 description 1
- 210000005228 liver tissue Anatomy 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000000874 microwave-assisted extraction Methods 0.000 description 1
- 229940096402 milk thistle seed Drugs 0.000 description 1
- 208000015994 miscarriage Diseases 0.000 description 1
- 210000004279 orbit Anatomy 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 229960005489 paracetamol Drugs 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 230000035807 sensation Effects 0.000 description 1
- 235000019615 sensations Nutrition 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 208000000995 spontaneous abortion Diseases 0.000 description 1
- 150000005846 sugar alcohols Chemical class 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
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- 239000003053 toxin Substances 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/115—Fatty acids or derivatives thereof; Fats or oils
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/20—Agglomerating; Granulating; Tabletting
- A23P10/28—Tabletting; Making food bars by compression of a dry powdered mixture
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
A kind of silybum marianum seed oil polypeptide mixture and preparation method thereof, is related to field of food.What the silybum marianum seed oil polypeptide mixture included counting in parts by weight following prepares raw material：20 50 parts of turmeric corn peptide；1 20 parts of silybum marianum seed oil；10 50 parts of D-sorbite；1 20 parts of microcrystalline cellulose；0.1 2 parts of 0.1 2 parts of magnesium stearate and silica；Wherein, turmeric corn peptide is prepared by turmeric and corn peptide, and the mass ratio of turmeric and corn peptide is 1 20：20‑50.Silybum marianum seed oil polypeptide mixture provided by the invention has alcohol-induced hepatic injury prevention and protective effect well.In addition the invention further relates to the preparation methods of above-mentioned silybum marianum seed oil polypeptide mixture.
Description
Technical field
The present invention relates to field of food, and more particularly to a kind of silybum marianum seed oil polypeptide mixture and preparation method thereof.
Background technology
Corn peptide is the product that zein hydrolyzes, not only containing the necessary various amino acid of human body, and easily
It is absorbed by the body, there is antifatigue, blood pressure lowering, liver protection, improve the multiple efficacies such as immunity of organisms, receive pushing away extensively for people
It is high.But corn peptide is prepared by raw material of zein, and corn is easily polluted be subject to zearalenone, corn is red
Mould ketenes is a kind of biotoxin with specific toxicity, with estrogen-like effect, can cause animal miscarriage, stillborn foetus,
The Reproductive Performances such as feelings exception is returned, the symptoms such as growth decline, immunosupress, infertility, deformity are may further result in, using by Gibberella zeae
The corn of ketenes pollution prepares corn peptide and easily zearalenone is introduced into corn peptide, human body is damaged, in addition
Corn peptide has strong bitter taste, this is because the hydrophobic group of hydrophobic amino acid is sudden and violent in the corn peptide that zein hydrolysis generates
Caused by dew, which also limits the uses of corn peptide.
Silybum marianum seed oil is that the dry mature fruit of feverfew milk thistle is prepared, and silybum marianum seed oil is rich in unsaturation
Aliphatic acid and linoleic acid can be effectively protected the effect of liver cell is encroached on from toxicant, and the antioxygen with strength
Change function, liver cell can be protected to be destroyed from free radical and accelerate to manufacture new liver cell, therefore be referred to as " natural guarantor
Liver medicine ".But presently disclosed document report shows the essences such as the research of silybum marianum seed oil is concentrated mainly on security, degumming refines
The measure of sweetening process, microwave-assisted extraction technique and double guest's contents, discloses less in terms of the products application of silybum marianum seed oil, does not have more
There is the open product being used cooperatively using silybum marianum seed oil and corn peptide.
The silybum marianum seed oil production of beneficiating ingredient in silybum marianum seed oil and corn peptide can be effectively utilized therefore, it is necessary to a kind of
Product.
The content of the invention
It is an object of the invention to provide a kind of silybum marianum seed oil polypeptide mixture, effectively can prevent and improve alcohol
Caused hepatic injury.
It, can be effective another object of the present invention is to provide a kind of preparation method of silybum marianum seed oil polypeptide mixture
Merge the beneficiating ingredient in each raw material.
The present invention is solved its technical problem and is realized using following technical scheme.
A kind of silybum marianum seed oil polypeptide mixture, is counted in parts by weight, and preparing raw material includes：20-50 parts of turmeric corn peptide；
1-20 parts of silybum marianum seed oil；10-50 parts of D-sorbite；1-20 parts of microcrystalline cellulose；0.1-2 parts of magnesium stearate and silica
0.1-2 parts；Wherein, turmeric corn peptide is prepared by turmeric and corn peptide, and the mass ratio of turmeric and corn peptide is 1-20: 20-
50。
Further, in present pre-ferred embodiments, count in parts by weight, preparing raw material includes：Turmeric corn peptide
25-45 parts；3-18 parts of silybum marianum seed oil；15-40 parts of D-sorbite；3-15 parts of microcrystalline cellulose；0.1-2 parts of magnesium stearate and
0.1-2 parts of silica.
The present invention also provides the preparation methods of above-mentioned silybum marianum seed oil polypeptide mixture, comprise the following steps：
It counts in parts by weight, by 20-50 parts of corn peptides and 1-20 portions of turmerics mix, granulation obtains turmeric corn peptide；
20-50 parts of turmeric corn peptides with 1-20 parts of silybum marianum seed oils are mixed, shear to obtain oil phase using colloid mill；
By 10-50 parts of D-sorbites, 1-20 parts of microcrystalline celluloses mix, dry sieve gets powder phase；
Oil phase and powder are mixed, then add in 0.1-2 parts of silica, 0.1-2 parts of magnesium stearates are stirred evenly, pressed
Piece.
Further, in present pre-ferred embodiments, granulation is to be put into after mixing corn peptide and turmeric in ebullated bed,
It is handled at 150-190 DEG C of 0.3-0.5mpa and inlet air temperature, 60-70 DEG C of leaving air temp by the use of water as binding agent granulation.
Further, in present pre-ferred embodiments, dry screening is after mixing D-sorbite and microcrystalline cellulose
16 mesh sieves are crossed, extracting screen underflow is in 60-70 DEG C of dry 1-2h, subsequent 14 mesh sieve extracting screen underflow of mistake.
Further, in present pre-ferred embodiments, it is to stir 30-60min under 19-25r/min to stir evenly.
Further, in present pre-ferred embodiments, corn peptide is prepared by following steps：
Zein and water by 1: 5-20 weight ratio are mixed evenly, 80-100 DEG C is warming up to and obtains feed liquid, with
Afterwards using the pH of calcium hydroxide aqueous solution adjusting feed liquid to 8.0-9.0, and keep the temperature 30-50min and obtain enzymolysis stoste；
The alkali protease that its weight 0.1%-3% is added in into enzymolysis stoste digests 1-1.5h, and it is former then to add in enzymolysis
The papain enzymolysis 1.5-2h of liquid weight 1%-5% is eventually adding the food flavor enzyme of enzymolysis stoste weight 0.05%-0.4%,
Enzymolysis 0.5-1h obtains enzymolysis liquid；
By enzymolysis liquid successively by enzyme deactivation, filtering, concentration, drying process.
Further, it is before adding in alkali protease into enzymolysis stoste, enzymolysis is former in present pre-ferred embodiments
The solid-liquid ratio of liquid is adjusted to 1: 5-30, is then cooled to 45-60 DEG C and adjusts pH to 7.0-8.0.
Further, in present pre-ferred embodiments, enzyme deactivation is to keep the temperature 15- after enzymolysis liquid is heated to 80-90 DEG C
30min。
Further, in present pre-ferred embodiments, adsorbed before being concentrated after enzymolysis liquid filtering, absorption is to mistake
The activated carbon of its weight 4%-6% is added in enzymolysis liquid after filter, and 30-90min is stirred at 45-55 DEG C.
The advantageous effect of the silybum marianum seed oil polypeptide mixture of the embodiment of the present invention and preparation method thereof is：It is provided by the invention
What silybum marianum seed oil polypeptide mixture included counting in parts by weight following prepares raw material：20-50 parts of turmeric corn peptide；Silybum marianum seed oil
1-20 parts；10-50 parts of D-sorbite；1-20 parts of microcrystalline cellulose；0.1-2 parts of 0.1-2 parts of magnesium stearate and silica；Its
In, turmeric corn peptide is prepared by turmeric and corn peptide, and the mass ratio of turmeric and corn peptide is 1-20: 20-50.The present invention carries
The silybum marianum seed oil polypeptide mixture of confession has good preventive and therapeutic action to alcohol-induced hepatic injury.It is provided by the invention
Silybum marianum seed oil polypeptide mixture preparation method can sufficiently mix and retain in corn peptide, turmeric and silybum marianum seed oil beneficial into
Point.
Specific embodiment
It, below will be in the embodiment of the present invention to make the purpose, technical scheme and advantage of the embodiment of the present invention clearer
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, builds according to normal condition or manufacturer
The condition of view carries out.Reagents or instruments used without specified manufacturer is the conventional production that can be obtained by commercially available purchase
Product.
Below to corn peptide of the embodiment of the present invention and preparation method thereof, silybum marianum seed oil polypeptide mixture and preparation method thereof
It is specifically described.
The present invention also provides a kind of silybum marianum seed oil polypeptide mixture, count in parts by weight, and preparing raw material includes：Turmeric
20-50 parts of corn peptide；1-20 parts of silybum marianum seed oil；10-50 parts of D-sorbite；1-20 parts of microcrystalline cellulose；Magnesium stearate 0.1-2
0.1-2 parts of part and silica；Wherein, turmeric corn peptide is prepared by turmeric and corn peptide, the matter of turmeric and corn peptide
Amount is than being 1-20: 20-50.
The silybum marianum seed oil polypeptide mixture uses corn peptide, turmeric and silybum marianum seed oil as principle active component, can
The advantages of summary raw material, has alcohol-induced hepatic injury prevention and protective effect well；Wherein corn peptide can
Promote metabolism of the liver to alcohol, assist to reduce alcohol to the damage of liver, avoid or alleviate the discomforts of dizziness and nausea and vomiting
Feel, and can help to releive awake from a drunken sleep after feel dizzy and the uncomfortable sensation of DOMS, there is excellent liver protecting effect.Ginger
Huang is the rhizome of the herbaceos perennial of Musales Zingiber, and containing abundant curcumin, and curcumin is to carbon tetrachloride, Huang
Aspergillin B1, paracetamol, iron and cyclophosphamide induction hepatic injury be effectively protected effect, can be with corn peptide
The hepatic injury of user is prevented and protective effect with silybum marianum seed oil cooperation.Silybum marianum seed oil is rich in unrighted acid
And linoleic acid, the effect of liver cell is encroached on from toxicant, and the anti-oxidation function with strength can be effectively protected,
Liver cell can be protected to be destroyed from free radical and accelerate to manufacture new liver cell, have to hepar damnification preferable prevention and
Protecting effect.
The present invention also provides the preparation methods of above-mentioned silybum marianum seed oil polypeptide mixture, comprise the following steps：
It counts in parts by weight, by 20-50 parts of corn peptides and 1-20 portions of turmerics mix, granulation obtains turmeric corn peptide；It is preferred that
, granulation is to be put into after mixing corn peptide and turmeric in ebullated bed, in 150-190 DEG C of 0.3-0.5mpa and inlet air temperature, is gone out
It is handled at 60-70 DEG C of air temperature by the use of water as binding agent granulation.
20-50 parts of turmeric corn peptides with 1-20 parts of silybum marianum seed oils are mixed, shear to obtain oil phase using colloid mill；
By 10-50 parts of D-sorbites, 1-20 parts of microcrystalline celluloses mix, dry sieve gets powder phase；Preferably, it is dry
Screening is to cross 16 mesh sieves after mixing D-sorbite and microcrystalline cellulose, and extracting screen underflow is in 60-70 DEG C of dry 1-2h, subsequent mistake 14
Mesh sieve extracting screen underflow.
Oil phase and powder are mixed, then add in 0.1-2 parts of silica, 0.1-2 parts of magnesium stearates are stirred evenly, pressed
Piece obtains silybum marianum seed oil polypeptide mixture.Preferably, stir evenly is to stir 30-60min under 19-25r/min.
Above-mentioned corn peptide is preferably prepared by following methods：
Zein and water by 1: 5-20 weight ratio are mixed evenly, 80-100 DEG C is warming up to and obtains feed liquid, with
Afterwards using the pH of calcium hydroxide aqueous solution adjusting feed liquid to 8.0-9.0, and keep the temperature 30-50min and obtain enzymolysis stoste；
The alkali protease that its weight 0.1%-3% is added in into enzymolysis stoste digests 1-1.5h, and it is former then to add in enzymolysis
The papain enzymolysis 1.5-2h of liquid weight 1%-5% is eventually adding the food flavor enzyme of enzymolysis stoste weight 0.05%-0.4%,
Enzymolysis 0.5-1h obtains enzymolysis liquid；Preferably, before adding in alkali protease into enzymolysis stoste, the solid-liquid ratio of stoste will be digested
1: 5-30 is adjusted to, be then cooled to 45-60 DEG C and adjusts pH to 7.0-8.0.
By enzymolysis liquid successively by enzyme deactivation, filtering, concentration, drying process.Preferably, enzyme deactivation is to be heated to enzymolysis liquid
15-30min is kept the temperature after 80-90 DEG C.It is furthermore preferred that being adsorbed before being concentrated after enzymolysis liquid filtering, absorption is after filtering
The activated carbon of its weight 4%-6% is added in enzymolysis liquid, and 30-90min is stirred at 45-55 DEG C.
Zein and water are mixed evenly the preparation method of corn peptide provided by the invention, use weak base hydroxide
Calcium adjust feed liquid pH zein is made fully to be denatured solidification, then further adjusting feed liquid solid-to-liquid ratio, temperature and pH value to
Alkali protease, papain and food flavor enzyme after OK range successively using suitable amounts carry out at enzymolysis zein
Reason obtains the enzymolysis liquid containing corn peptide, is distributed and added using a variety of enzymes, the technique that gradient hydrolyzes successively can be to greatest extent
The generation of free amino acid is limited, so as to effectively reduce the content of hydrophobic amino acid in corn peptide, to reduce obtained jade
The bitter taste of rice peptide；Enzymolysis liquid is then obtained into corn peptide by enzyme deactivation, filtering, concentration, drying process successively, in filter process and
The zearalenone toxin that may contain in enzymolysis liquid can be effectively removed using activated carbon adsorption between concentration process, is protected
Demonstrate,prove the safe to use of corn peptide.
The preparation method of silybum marianum seed oil polypeptide mixture provided by the invention is suitable first by turmeric and corn peptide
At a temperature of mixing granulation obtain turmeric corn peptide, sheared after then turmeric corn peptide is mixed with silybum marianum seed oil using colloid mill
Crushing is uniformly mixed so as to obtain rich in beneficiating ingredient and is sufficiently mixed uniform oil phase, after D-sorbite and microcrystalline cellulose combination drying
The screenings of 14 mesh is taken to obtain the powder phase that grain graininess is uniform and surface area is big by sieving twice, finally by powder phase and oil
Mix, and add in silica and magnesium stearate filling be prepared into silybum marianum seed oil polypeptide mixture, above-mentioned preparation method is not only
The amino acid contained in the curcumin and corn peptide that can contain to avoid turmeric in raw material is lost in and is sufficiently mixed the two obtained
Turmeric corn peptide can also use silybum marianum seed oil and be fully pulverized and mixed by colloid mill uniformly with turmeric corn peptide, makes three kinds
Beneficiating ingredient sufficiently mixes blending in raw material, so as to effectively improve the absorption rate of human body, and significantly improves to making
The protective effect of user's liver.
The feature and performance of the present invention are described in further detail with reference to embodiments.
Embodiment 1
A kind of preparation method of corn peptide is present embodiments provided, preparation process is as follows：
Zein and water by 1: 10 weight ratio are mixed evenly, 90 DEG C is warming up to and obtains feed liquid, is then used
Calcium hydroxide aqueous solution adjusts the pH of feed liquid to 8.5, and keeps the temperature 40min and obtain enzymolysis stoste, adds water that will digest the feed liquid of stoste
Than being adjusted to 1: 20, then it is cooled to 50 DEG C and adjusts pH to 7.5；
The alkali protease that its weight 1.5% is added in into enzymolysis stoste digests 1h, then adds in enzymolysis stoste weight 3%
Papain enzymolysis 1.5h, be eventually adding the food flavor enzyme of enzymolysis stoste weight 0.2%, enzymolysis 0.5h obtains enzymolysis liquid；
Enzymolysis liquid is heated to keeping the temperature 20min enzyme deactivations, filtering successively, into the enzymolysis liquid after filtering to add in its heavy after 90 DEG C
The activated carbon of amount 5%, and removal activated carbon, concentration, drying process are filtered after stirring 45min absorption at 50 DEG C, obtain corn
Peptide.
Embodiment 2
A kind of preparation method of corn peptide is present embodiments provided, preparation process is as follows：
Zein and water by 1: 15 weight ratio are mixed evenly, 80 DEG C is warming up to and obtains feed liquid, is then used
Calcium hydroxide aqueous solution adjusts the pH of feed liquid to 9.0, and keeps the temperature 45min and obtain enzymolysis stoste, adds water that will digest the feed liquid of stoste
Than being adjusted to 1: 10, then it is cooled to 55 DEG C and adjusts pH to 8；
The alkali protease that its weight 0.8% is added in into enzymolysis stoste digests 1.5h, then adds in enzymolysis stoste weight
1.5 papain enzymolysis 2h, is eventually adding the food flavor enzyme of enzymolysis stoste weight 0.15%, and enzymolysis 1h obtains enzymolysis liquid；
Enzymolysis liquid is heated to keeping the temperature 25min enzyme deactivations, filtering successively, into the enzymolysis liquid after filtering to add in its heavy after 85 DEG C
The activated carbon of amount 6%, and removal activated carbon, concentration, drying process are filtered after stirring 45min absorption at 50 DEG C, obtain corn
Peptide.
Embodiment 3
A kind of preparation method of corn peptide is present embodiments provided, preparation process is as follows：
Zein and water by 1: 10 weight ratio are mixed evenly, 100 DEG C is warming up to and obtains feed liquid, is then used
Calcium hydroxide aqueous solution adjusts the pH of feed liquid to 9.0, and keeps the temperature 35min and obtain enzymolysis stoste, adds water that will digest the feed liquid of stoste
Than being adjusted to 1: 9, then it is cooled to 55 DEG C and adjusts pH to 8；
The alkali protease that its weight 2% is added in into enzymolysis stoste digests 1.5h, then adds in enzymolysis stoste weight
3.5% papain enzymolysis 2h, is eventually adding the food flavor enzyme of enzymolysis stoste weight 0.25%, and enzymolysis 1h obtains enzymolysis liquid；
Enzymolysis liquid is heated to keeping the temperature 30min enzyme deactivations, filtering successively, into the enzymolysis liquid after filtering to add in its heavy after 90 DEG C
The activated carbon of amount 4%, and removal activated carbon, concentration, drying process are filtered after stirring 55min absorption at 55 DEG C, obtain corn
Peptide.
Embodiment 4
A kind of preparation method of silybum marianum seed oil polypeptide mixture is present embodiments provided, preparation process is as follows：
After the corn peptide that 3000g embodiments 1 are prepared and the mixing of 1000g turmerics in input ebullated bed, in 0.5mpa
With 160 DEG C of inlet air temperature, turmeric corn peptide is prepared by the use of water as binding agent granulation processing at 70 DEG C of leaving air temp.
250g turmerics corn peptide with 100g silybum marianum seed oils is mixed, oil phase is obtained using colloid mill shearing 1.2h；
By 250g D-sorbites, 100g microcrystalline celluloses mix, cross 16 mesh sieves, extracting screen underflow in 60 DEG C of dry 1.5h, with
14 mesh sieve extracting screen underflows are crossed afterwards obtains powder phase；
Oil phase and powder are mixed, then add in 10g silica, 10g magnesium stearates stir under 20r/min
30min, tabletting obtain silybum marianum seed oil polypeptide mixture.
Embodiment 5
A kind of preparation method of silybum marianum seed oil polypeptide mixture is present embodiments provided, preparation process is as follows：
After the corn peptide that 5000g embodiments 1 are prepared and the mixing of 1000g turmerics in input ebullated bed, in 0.4mpa
With 180 DEG C of inlet air temperature, handle to obtain turmeric corn peptide by the use of water as binding agent granulation at 60 DEG C of leaving air temp.
500g turmerics corn peptide with 200g silybum marianum seed oils is mixed, oil phase is obtained using colloid mill shearing 2h；
By 300g D-sorbites, 200g microcrystalline celluloses mix, dry sieve gets powder phase；Dry screening is by sorb
16 mesh sieves are crossed after sugar alcohol and microcrystalline cellulose mixing, extracting screen underflow is in 65 DEG C of dry 2h, subsequent 14 mesh sieve extracting screen underflow of mistake.
Oil phase and powder are mixed, then add in 20g silica, 20g magnesium stearates stir under 25r/min
45min, tabletting obtain silybum marianum seed oil polypeptide mixture.
Embodiment 6
A kind of preparation method of silybum marianum seed oil polypeptide mixture is present embodiments provided, preparation process is as follows：
After the corn peptide that 3500g embodiments 2 are prepared and the mixing of 800g turmerics in input ebullated bed, in 0.35mpa
With 165 DEG C of inlet air temperature, handle to obtain turmeric corn peptide by the use of water as binding agent granulation at 70 DEG C of leaving air temp.
350g turmerics corn peptide with 120g silybum marianum seed oils is mixed, oil phase is obtained using colloid mill shearing 1.5h；
350g D-sorbites, 150g microcrystalline celluloses are crossed into 16 mesh sieves after mixing, extracting screen underflow is in 70 DEG C of dry 2h, then
It crosses 14 mesh sieve extracting screen underflows and obtains powder phase.
Oil phase and powder are mixed, then add in 15g silica, 15g magnesium stearates stir under 24r/min
60min, tabletting obtain silybum marianum seed oil polypeptide mixture.
Embodiment 7
A kind of preparation method of silybum marianum seed oil polypeptide mixture is present embodiments provided, preparation process is as follows：
After the corn peptide that 3000g embodiments 2 are prepared and the mixing of 1000g turmerics in input ebullated bed, in 0.4mpa
With 170 DEG C of inlet air temperature, handle to obtain turmeric corn peptide by the use of water as binding agent granulation at 60 DEG C of leaving air temp.
380g turmerics corn peptide with 140g silybum marianum seed oils is mixed, oil phase is obtained using colloid mill shearing 1h；
280g D-sorbites, 130g microcrystalline celluloses are crossed into 16 mesh sieves after mixing, extracting screen underflow in 65 DEG C of dry 1.5h, with
14 mesh sieve extracting screen underflows are crossed afterwards obtains powder phase.
Oil phase and powder are mixed, then add in 20g silica, 15g magnesium stearates stir under 20r/min
50min, tabletting obtain silybum marianum seed oil polypeptide mixture.
Embodiment 8
A kind of preparation method of silybum marianum seed oil polypeptide mixture is present embodiments provided, preparation process is as follows：
After the corn peptide that 4700g embodiments 1 are prepared and the mixing of 1500g turmerics in input ebullated bed, in 0.35mpa
With 150 DEG C of inlet air temperature, handle to obtain turmeric corn peptide by the use of water as binding agent granulation at 70 DEG C of leaving air temp.
240g turmerics corn peptide with 60g silybum marianum seed oils is mixed, shears to obtain oil phase using colloid mill；
150g D-sorbites, 80g microcrystalline celluloses are crossed into 16 mesh sieves after mixing, extracting screen underflow is in 65 DEG C of dry 2h, then
It crosses 14 mesh sieve extracting screen underflows and obtains powder phase.
Oil phase and powder are mixed, then add in 10g silica, 10g magnesium stearates stir under 21r/min
40min, tabletting obtain silybum marianum seed oil polypeptide mixture.
Corn peptide provided in an embodiment of the present invention, silybum marianum seed oil polypeptide mixture are detected below.
1. the corn peptide that pair embodiment of the present invention 1 is prepared carries out Physico-chemical tests, the results are shown in Table 1, physics and chemistry inspection
Survey is carried out by Guangzhou Product Quality Verification Centers：
The Physico-chemical tests result for the corn peptide that 1 embodiment of the present invention 1 of table provides
2. the silybum marianum seed oil polypeptide mixture that pair embodiment of the present invention 4 provides carries out zoopery.
2.1 test method：Animal subject ICR mouse 80 are chosen, male, 20 ± 2g of weight, after adaptability feeds 3d, with
Machine is bisected into 8 groups, is respectively blank group, model group, silybum marianum seed oil polypeptide dosage group (recommend dosage respectively with human body 10
Times, 20 times, 30 multiple doses be silybum marianum seed oil polypeptide mixture that content is provided using embodiment 4 be low dose group, middle dose group,
High dose group), corn peptide group (using corn peptide content in middle dose group using the corn peptide that embodiment 1 provides as given low),
Turmeric group (using turmeric as given low using turmeric content in middle dose group), silybum marianum seed oil group are (with fine grinding in middle dose group
Ji seed oil content is given low using silybum marianum seed oil), daily gastric infusion 1 time, continuous 30d, blank group and model group are given
Give distilled water, after last gavage, equal 1 gavage of remaining each group gives 50% ethyl alcohol (12mL/kg) in addition to blank group, and blank group is given
Give the distilled water of same dose, after fasting 16h, eye socket blood sampling detects the enzymatic activity and TC, TG of AKP, ALT, AST in serum
Content.Liver is taken to measure MDA, GSH content in liver organization, result is as shown in the following table 1, table 2 and table 3：
The variation of AKP, ALT, AST enzymatic activity in 1 each group mice serum of table
The variation of TG, TC content in 2 each group mice serum of table
| Group | TG contents (mmol/L) | TC contents (mmol/L) |
| Blank group | 0.74±0.26** | 2.74±0.42** |
| Model group | 1.62±0.47 | 4.36±0.81 |
| Low dose group | 1.49±0.22* | 3.97±1.21 |
| Middle dose group | 1.21±0.56* | 3.24±0.51* |
| High dose group | 0.98±0.32** | 2.99±1.74** |
| Corn peptide group | 1.51±0.37 | 4.06±0.98 |
| Turmeric group | 1.47±0.41* | 4.08±1.13 |
| Silybum marianum seed oil group | 1.66±0.53 | 3.91±0.29 |
The variation of MDA, GSH in 3 each group murine liver tissue of table
| Group | MDA(nmol/mgpro) | GSH(mg/mgpro) |
| Blank group | 1.61±0.36** | 23.51±4.97** |
| Model group | 3.71±0.98 | 9.89±5.75 |
| Low dose group | 3.14±1.14* | 13.21±3.42* |
| Middle dose group | 2.98±0.63* | 18.67±6.18** |
| High dose group | 2.21±0.52** | 21.77±5.04** |
| Corn peptide group | 3.31±0.49 | 14.75±7.64* |
| Turmeric group | 3.23±0.87 | 11.98±5.53 |
| Silybum marianum seed oil group | 3.64±0.91 | 12.98±4.73 |
To sum up shown, in the results show naive mice serum, AKP, ALT, AST activity are relatively below mould with model group
Type group and difference have pole conspicuousness, illustrate that the liver cell of model group mouse is damaged, alcoholic liver injury modeling success.It is low, in,
High three dosage groups AKP, ALT, AST enzymatic activity is below model group compared with model group, and difference has conspicuousness, corn
Peptide group AKP, AST enzymatic activity is less than model group and with otherness, but activity is higher than middle dose group, turmeric group and silybum marianum seed oil
Group AKP, ALT, AST enzymatic activity do not have conspicuousness less than model group but difference.Middle and high dosage group serum TC, TG contents are low
Conspicuousness is presented in model group and difference, MDA, GSH content are above model group in basic, normal, high three dosage group liver organizations,
And difference has conspicuousness.Illustrate that silybum marianum seed oil polypeptide mixture provided by the invention can effectively protect the liver damage caused by alcohol
Wound, and effect is better than the same dose of simple corn peptides, turmeric and silybum marianum seed oil, it should be noted that the water under this dosage
Fly Ji seed oil and act on unobvious for the liver injury protection caused by alcohol, but the functionality of product is substantially strengthened after compatibility, shows
Silybum marianum seed oil polypeptide mixture provided by the invention compares single milk thistle using corn peptide, turmeric with silybum marianum seed oil cooperation
Seed oil has better liver injury protection effect.
In conclusion silybum marianum seed oil polypeptide mixture provided in an embodiment of the present invention uses corn peptide, turmeric and milk thistle
Seed oil cooperation, has alcohol-induced hepatic injury prevention and protective effect well；Silybum marianum seed oil disclosed by the invention is more
Oligopeptide mixture preparation method can be mixed effectively and retain the beneficiating ingredient in corn peptide, turmeric and silybum marianum seed oil, and be passed through
The collaboration absorption of human body improves the absorption rate of silybum marianum seed oil polypeptide mixture.
Embodiments described above is part of the embodiment of the present invention, instead of all the embodiments.The reality of the present invention
The detailed description for applying example is not intended to limit the scope of claimed invention, but is merely representative of the selected implementation of the present invention
Example.Based on the embodiments of the present invention, those of ordinary skill in the art are obtained without creative efforts
Every other embodiment, belongs to the scope of protection of the invention.
Claims (10)
1. a kind of silybum marianum seed oil polypeptide mixture, which is characterized in that count in parts by weight, preparing raw material includes：
Wherein, the turmeric corn peptide is prepared by turmeric and corn peptide, and the mass ratio of the turmeric and the corn peptide is
1-20：20-50.
2. silybum marianum seed oil polypeptide mixture according to claim 1, which is characterized in that count, prepare former in parts by weight
Material includes：
3. a kind of preparation method of silybum marianum seed oil polypeptide mixture, which is characterized in that it comprises the following steps：
It counts in parts by weight, by 20-50 parts of corn peptides and 1-20 portions of turmerics mix, granulation obtains turmeric corn peptide；
The 20-50 parts of turmeric corn peptides with 1-20 parts of silybum marianum seed oils are mixed, shear to obtain oil phase using colloid mill；
By 10-50 parts of D-sorbites, 1-20 parts of microcrystalline celluloses mix, dry sieve gets powder phase；
The oil phase and the powder are mixed, then add in 0.1-2 parts of silica, 0.1-2 parts of magnesium stearate stirrings
Even, tabletting.
4. the preparation method of silybum marianum seed oil polypeptide mixture according to claim 3, which is characterized in that it is described granulation be by
It is put into after the corn peptide and turmeric mixing in ebullated bed, in 150-190 DEG C of 0.3-0.5mpa and inlet air temperature, goes out wind-warm syndrome
It is handled at 60-70 DEG C of degree by the use of water as binding agent granulation.
5. the preparation method of silybum marianum seed oil polypeptide mixture according to claim 3, which is characterized in that the dry screening
Be will the D-sorbite and the microcrystalline cellulose mix after cross 16 mesh sieves, extracting screen underflow is in 60-70 DEG C of dry 1-2h, then
Cross 14 mesh sieve extracting screen underflows.
6. the preparation method of silybum marianum seed oil polypeptide mixture according to claim 3, which is characterized in that described to stir evenly
It is to stir 30-60min under 19-25r/min.
7. the preparation method of silybum marianum seed oil polypeptide mixture according to claim 3, which is characterized in that the corn peptide by
Following steps are prepared：
Zein and water are pressed 1：The weight ratio of 5-20 is mixed evenly, and is warming up to 80-100 DEG C and obtains feed liquid, then makes
With the pH of the calcium hydroxide aqueous solution adjusting feed liquid to 8.0-9.0, and keep the temperature 30-50min and obtain enzymolysis stoste；
The alkali protease that its weight 0.1%-3% is added in into the enzymolysis stoste digests 1-1.5h, then adds in the enzyme
The papain enzymolysis 1.5-2h of stoste weight 1%-5% is solved, is eventually adding the enzymolysis stoste weight 0.05%-0.4%
Food flavor enzyme, enzymolysis 0.5-1h obtain enzymolysis liquid；
By the enzymolysis liquid successively by enzyme deactivation, filtering, concentration, drying process.
8. the preparation method of silybum marianum seed oil polypeptide mixture according to claim 7, which is characterized in that former to the enzymolysis
It is added in liquid before the alkali protease, the solid-liquid ratio of the enzymolysis stoste is adjusted to 1：5-30 is then cooled to 45-60
DEG C and adjust pH to 7.0-8.0.
9. the preparation method of silybum marianum seed oil polypeptide mixture according to claim 7, which is characterized in that the enzyme deactivation be by
The enzymolysis liquid keeps the temperature 15-30min after being heated to 80-90 DEG C.
10. the preparation method of silybum marianum seed oil polypeptide mixture according to claim 7, which is characterized in that in the enzymolysis
It is adsorbed before being concentrated after liquid filtering, the absorption is that the activated carbon of its weight 4%-6% is added in the enzymolysis liquid after filtering,
And stir 30-90min at 45-55 DEG C.
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|---|---|---|---|---|
| CN104593318A (en) * | 2013-10-31 | 2015-05-06 | 中国食品发酵工业研究院 | A corn bioactive peptide additive used for a cell culture medium |
| CN105942497A (en) * | 2016-05-04 | 2016-09-21 | 江苏神华药业有限公司 | Composition with effects of neutralizing effect of alcoholic drinks and nourishing liver and preparation method of composition |
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|---|---|---|---|---|
| CN104593318A (en) * | 2013-10-31 | 2015-05-06 | 中国食品发酵工业研究院 | A corn bioactive peptide additive used for a cell culture medium |
| CN105942497A (en) * | 2016-05-04 | 2016-09-21 | 江苏神华药业有限公司 | Composition with effects of neutralizing effect of alcoholic drinks and nourishing liver and preparation method of composition |
| CN106334181A (en) * | 2016-08-23 | 2017-01-18 | 江苏朸健生命科技发展有限公司 | Tablet for protecting liver and relieving effect of alcohol and preparation method thereof |
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