CN108077325B - Plant growth promoter - Google Patents
Plant growth promoter Download PDFInfo
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- CN108077325B CN108077325B CN201711348558.9A CN201711348558A CN108077325B CN 108077325 B CN108077325 B CN 108077325B CN 201711348558 A CN201711348558 A CN 201711348558A CN 108077325 B CN108077325 B CN 108077325B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
The invention provides a plant growth promoter, which contains water extract of dried powder of aerial parts of German chamomile, hyssop and tanacetum vulgare. When the growth promoting agent is applied to plants, particularly vegetable plants, such as Chinese cabbage, rape, lettuce and other leaf vegetables, the height of the leaf vegetables applied with the growth promoting agent is increased by 41.5 percent, the number of leaves is increased by 24 percent, the area of the leaves is increased by 201 percent, the dry weight of the overground part is increased by 75.2 percent, and the total dry weight is increased by 70.4 percent when the growth promoting agent is harvested compared with the non-applied plants. Compared with the conventional fertilizer, the growth promoting effect of the leaf vegetables is close, and the growth promoting agent has more ecological significance. In actual production, the method can replace conventional fertilization, thereby reducing the application of nitrogen and relieving the residue problem caused by excessive application of the nitrogen fertilizer to the soil at present.
Description
Technical Field
The invention belongs to the field of plant allelopathy, and particularly relates to a plant growth promoter.
Background
Allelopathy was first suggested by the scientist Molisch in 1937, the International association Society of Allelopathy (IAS) in 1996 in 1 st world meeting in spain, and in 2011 in china in 6 th. Plant allelopathy is the direct or indirect, harmful or beneficial effect of plants on surrounding plants by releasing chemical substances to the environment through leaching, volatilization, residue decomposition, root secretion and other modes. Allelopathy mainly manifests in influencing cell division, elongation and the fine structure of root tip, influencing the permeability of cell membrane, influencing the absorption of mineral ions, influencing photosynthesis, respiration and the like. At present, research on allelochemicals at home and abroad separates and identifies some allelochemicals and gradually deepens into the mechanism aspect. The allelopathy is ubiquitous in the nature, and the intensive research on the allelopathy is beneficial to the scientification of plant configuration, cultivation system and cultivation measures in a compound system, the promotion effect among plants is enhanced, the inhibition effect is reduced, and the biological diversity and agricultural sustainable development are promoted.
China is already the first major country in the world for nitrogen fertilizer production and consumption. The production of nitrogen fertilizer accounts for 1/3 of the total world production, and 7% of the world's land consumes 35% of the global nitrogen fertilizer. With the increasing of the output and application amount of nitrogen fertilizer, the total output of the main grain crops such as wheat, corn and rice in China is increased by nearly ten times. However, excessive application of nitrogen fertilizer results in reduced fertilizer efficiency, reduced yield increase amplitude and reduced crop edible safety. Meanwhile, the residual quantity of the nitrogen fertilizer in the soil is obviously increased, so that the carbon-nitrogen ratio of the soil is disordered, and the microbial environment of the soil is damaged. The nitrogen fertilizer usage amount per hectare farm land in China reaches more than 400 kilograms, which is far higher than the upper safety limit of 225 kilograms in developed countries. The environmental losses caused by excessive use of nitrogen fertilizers have been greater than the gains of increased grain yields. Therefore, the search for vegetal fertilizers to replace nitrogen fertilizers has been very urgent.
Xue Qi et al (2017) uses wrinkled giant hyssop with obvious allelopathy as material, and uses water extract to simulate the mode of allelopathy of rain and fog leaching under natural condition, and uses water extract with different mass concentration of each part of wrinkled giant hyssop leaf, stem and root to treat wrinkled giant hyssop seed and seedling, and detect the change of relevant physiological and biochemical indexes. The results show that the treatment of the water extract with different mass concentrations of each part of the ageratum leaves, stems and roots reduces the root activity, chlorophyll content and soluble protein content of the ageratum seedlings, inhibits the activities of superoxide dismutase (SOD), Peroxidase (POD) and Catalase (CAT), and improves the relative conductivity of the leaves of the seedlings and the Malondialdehyde (MDA) content. The water extract at different parts can inhibit the germination of agastache rugosus seeds and the growth of seedlings, and shows obvious concentration gradient effect and part difference, the higher the concentration of the water extract is, the stronger the inhibition effect is, the strongest the inhibition effect of the water extract of the leaves on the seedlings after the water extract of the leaves is treated at the same concentration at different parts is, and the obvious level is achieved.
Saharkhiz et al, 2016, scoringThe phytotoxicity of catnip (Nepeta cataria L.) aerial Essential Oil (EO) on some harmful weeds and field crops was estimated in an attempt to explore the production potential of natural herbicides. The concentration of Nepeta catarrhalis essential oil is measured at 0,150,300,600 and 1200 mu L-1Inhibition of seed germination and seedling growth at concentrations in barley, dandelion, oat and three crop seeds including lipidiam sativum, catmint and basil. The results show different phytotoxicity and selectivity for germination and growth of the species studied.
S Mutlu and
Atici, 2009, to evaluate the allelopathic potential of Nepeta meyeri, the effect of water extracts (0.125,0.25,0.5,1,2.5 and 5%) prepared from the roots and leaves of Nepeta cataria on seed germination and seedling growth of several economically important crops (barley, wheat, oilseed rape, safflower and sunflower) was studied. Root and leaf extracts of n.meyeri caused general phytotoxic effects on seed germination and seedling growth of barley and sunflower at all concentrations. However, both root and leaf extracts significantly improved wheat seedling growth, particularly at lower concentrations of 0.125,0.25 and 0.5%, while higher concentrations had a neutral effect. The two extracts also have certain improvement on the seed germination and seedling growth of rape and safflower, especially at lower concentration. However, these advantages are not observed at higher concentrations, where the extract mostly has phytotoxic effects on rape and safflower. The allelochemical activity of nepeta cataria depends on whether the extract is derived from the leaf or root portions of a plant. The leaf extract has the greatest allelopathic effect. The results indicate that the water extract from nepeta cataria has chemosensory potential and should be evaluated as a chemosensory species, providing a risk or advantage for seed germination and seedling growth of crop or weed plants.
The tomato growth is promoted by intercropping and interplanting the aromatic plants such as the Chinese mugwort, 2014, the mint, the purple perilla, the basil and the like, and the tomato growth is influenced by different intercropping and interplanting combinations. The mint plants are very short compared with tomatoes, the difference between the heights of the basil plants and the perilla plants and the tomatoes is very small, the mint/tomato combination can obtain more light energy compared with other aromatic plants/tomatoes and tomatoes in single cropping, and the synergistic effect of mint intercropping and interplanting on the growth of the tomatoes is realized.
From the results of the present studies, it is known that allelochemicals having growth promoting effects on plants have been recently reported in the allelochemicals field.
Disclosure of Invention
In order to solve the above problems, the present invention provides a plant growth promoter.
First, the present invention provides a plant growth promoter comprising an aqueous extract of dried powders of aerial parts of chamomile, hyssop and tanacetum parthenium.
In one embodiment of the invention, the aqueous extract is prepared as follows: collecting aerial parts of German chamomile, hyssop and tanacetum vulgare, drying, grinding into powder, extracting in water at a weight ratio of 1:2-50, standing at 20-30 deg.C, soaking for 24-48h, and collecting filtrate.
Wherein the powder is preferably extracted in water at a weight ratio of 1: 5-20.
Wherein the mass ratio of the German chamomile, the hyssop and the chrysanthemum is 1-5:1-5: 1-5.
The plant growth promoters of the present invention may also comprise growth promoting substances known in the art, such as organic-inorganic fertilizers, and other conventional growth promoters known in the art.
The plants of the present invention are preferably field crops and vegetables, including but not limited to rice, wheat, corn, soybean, sorghum, etc., more preferably vegetables, including but not limited to rape, leaf lettuce, spinach, lettuce, Chinese cabbage, tomato, cucumber, etc., and most preferably leaf vegetables, including but not limited to rape, leaf lettuce, spinach, Chinese cabbage, etc.
The invention also provides a preparation method of the plant growth promoter, which comprises the steps of collecting the overground parts of German chamomile, hyssop and tanacetum vulgare, drying, grinding into powder, extracting in water according to the weight ratio of 1:2-50, standing and soaking at 20-30 ℃ for 24-48h, and taking the filtrate.
Wherein the powder is preferably extracted in water at a weight ratio of 1: 5-20.
Wherein the mass ratio of the German chamomile, the hyssop and the chrysanthemum is 1-5:1-5: 1-5.
The invention also provides a plant cultivation method, which is characterized in that after seeds germinate, the plant growth promoter is irrigated when the plant height reaches more than 2cm, the application amount per square meter is 2.5 liters, and the plant growth promoter is applied once per week.
When the growth promoter is applied to plants, particularly vegetable plants, such as Chinese cabbage, rape, lettuce and other leafy vegetables, the height of the leafy vegetables applied with the growth promoter is increased by 41.5 percent, the number of leaves is increased by 24 percent, the area of the leaves is increased by 201 percent, the dry weight of the overground part is increased by 75.2 percent, and the total dry weight is increased by 70.4 percent when the leafy vegetables are harvested. Compared with the conventional fertilizer, the growth promoting effect of the leaf vegetables is close, and the growth promoting agent has more ecological significance. In actual production, the method can replace conventional fertilization, thereby reducing the application of nitrogen and relieving the residue problem caused by excessive application of the nitrogen fertilizer to the soil at present.
Detailed Description
The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
Example 1 preparation of plant growth promoters
Collecting aerial parts of German chamomile, hyssop and tanacetum vulgare, collecting, drying in the shade, cutting into 2-3cm small segments, and pulverizing into powder for later use.
Uniformly mixing the powder of the German chamomile, the hyssop and the chrysanthemum in a weight ratio of 1:1:1, putting the mixture into water in a weight ratio of 1:20 for extraction, standing and soaking the mixture for 24 hours at 25 ℃, and filtering the mixture for 2 times by using gauze, wherein the filtrate is the plant growth promoter.
Example 2 preparation of plant growth promoters
Collecting aerial parts of German chamomile, hyssop and tanacetum vulgare, collecting, drying in the shade, cutting into 2-3cm small segments, and pulverizing into powder for later use.
Uniformly mixing the powder of the German chamomile, the hyssop and the chrysanthemum in a weight ratio of 1:2:1, putting the mixture into water in a weight ratio of 1:10 for extraction, standing and soaking the mixture for 24 hours at 25 ℃, and filtering the mixture for 2 times by using gauze, wherein the filtrate is the plant growth promoter.
Example 3 preparation of plant growth promoters
Collecting aerial parts of German chamomile, hyssop and tanacetum vulgare, collecting, drying in the shade, cutting into 2-3cm small segments, and pulverizing into powder for later use.
Uniformly mixing the powder of the German chamomile, the hyssop and the chrysanthemum in a weight ratio of 1:2:3, putting the mixture into water in a weight ratio of 1:30 for extraction, standing and soaking the mixture for 24 hours at 25 ℃, and filtering the mixture for 2 times by using gauze, wherein the filtrate is the plant growth promoter.
Example 4 preparation of plant growth promoters
Collecting aerial parts of German chamomile, hyssop and tanacetum vulgare, collecting, drying in the shade, cutting into 2-3cm small segments, and pulverizing into powder for later use.
Uniformly mixing the powder of the German chamomile, the hyssop and the chrysanthemum in a weight ratio of 2:2:5, putting the mixture into water in a weight ratio of 1:5 for extraction, standing and soaking at 25 ℃ for 24 hours, and filtering for 2 times by using gauze, wherein the filtrate is the plant growth promoter.
Example 5 preparation of plant growth promoters
Collecting aerial parts of German chamomile, hyssop and tanacetum vulgare, collecting, drying in the shade, cutting into 2-3cm small segments, and pulverizing into powder for later use.
Uniformly mixing the powder of the German chamomile, the hyssop and the chrysanthemum according to the weight ratio of 3:5:2, putting the mixture into water according to the weight ratio of 1:50 for extraction, standing and soaking the mixture for 24 hours at 25 ℃, and filtering the mixture for 2 times by using gauze, wherein the filtrate is the plant growth promoter.
Example 6 preparation of plant growth promoters
Collecting aerial parts of German chamomile, hyssop and tanacetum vulgare, collecting, drying in the shade, cutting into 2-3cm small segments, and pulverizing into powder for later use.
Uniformly mixing the powder of the German chamomile, the hyssop and the chrysanthemum according to the weight ratio of 2:1:4, putting the mixture into water according to the weight ratio of 1:2 for extraction, standing and soaking the mixture for 24 hours at 25 ℃, and filtering the mixture for 2 times by using gauze, wherein the filtrate is the plant growth promoter.
Comparative example 1
Collecting aerial parts of German chamomile, collecting, drying in the shade, cutting into 2-3cm small segments, and pulverizing into powder. Extracting in water at a weight ratio of 1:20, standing at 25 deg.C, soaking for 24 hr, filtering with gauze for 2 times, and collecting filtrate.
Comparative example 2
Collecting aerial parts of Hyssopus officinalis, collecting, drying in the shade, cutting into 2-3cm segments, and pulverizing into powder. Extracting in water at a weight ratio of 1:20, standing at 25 deg.C, soaking for 24 hr, filtering with gauze for 2 times, and collecting filtrate.
Comparative example 3
Collecting aerial parts of folium Artemisiae Argyi, collecting, drying in the shade, cutting into 2-3cm segments, and pulverizing into powder. Extracting in water at a weight ratio of 1:20, standing at 25 deg.C, soaking for 24 hr, filtering with gauze for 2 times, and collecting filtrate.
Comparative example 4
Collecting aerial parts of German chamomile and tanacetum vulgare, collecting, drying in the shade, cutting into 2-3cm small segments, and pulverizing into powder.
Uniformly mixing the powder of the German chamomile and the powder of the blumea balsamifera according to the weight ratio of 1:1, putting the mixture into water according to the weight ratio of 1:20 for extraction, standing and soaking the mixture for 24 hours at 25 ℃, filtering the mixture for 2 times by using gauze, and taking filtrate.
Comparative example 5
Collecting aerial parts of hyssop and folium Artemisiae Argyi, collecting, drying in the shade, cutting into 2-3cm small segments, and pulverizing into powder.
Mixing the powder of hyssop and the powder of the blumea according to the weight ratio of 1:1, putting the mixture into water according to the weight ratio of 1:20 for extraction, standing and soaking the mixture for 24 hours at 25 ℃, filtering the mixture for 2 times by using gauze, and taking filtrate.
Comparative example 6
Collecting aerial parts of German chamomile and hyssop, collecting, drying in the shade, cutting into 2-3cm small segments, and pulverizing into powder.
Mixing the powder of German chamomile and hyssop according to the weight ratio of 1:1, putting the mixture into water according to the weight ratio of 1:20 for extraction, standing and soaking the mixture for 24 hours at 25 ℃, filtering the mixture for 2 times by using gauze, and taking the filtrate.
Test example 1 field test
The test adopts a greenhouse field planting method. The greenhouse was sown in the same manner as usual, and when the plant height reached 2cm after the germination of the seed, the plant growth promoters (7 groups) prepared in example 1 and the filtrates (1-6 groups) prepared in comparative examples 1-6 were irrigated with 2.5L of water immersion per square meter to prepare treatment groups 1-7. The negative control CK1 was watered with an equal amount of tap water and all treatments were 7 days/1 time. And a fertilizer is used as a positive control CK 2. Data are collected before each irrigation and are recorded in tables 1-4. And (5) performing data analysis after all data are acquired, and drying and weighing the test material.
TABLE 1 Effect of different treatments on lettuce plant height (cm)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | CK1 | CK2 | |
| D0 | 3.63 | 3.72 | 3.68 | 3.36 | 3.82 | 3.54 | 3.70 | 3.81 | 3.71 |
| D7 | 6.77 | 7.12 | 5.20 | 5.68 | 5.98 | 6.67 | 7.26 | 5.00 | 5.29 |
| D14 | 9.23 | 9.83 | 7.17 | 8.72 | 9.34 | 9.88 | 9.80 | 7.03 | 7.57 |
| D21 | 10.69 | 11.10 | 8.82 | 10.10 | 10.65 | 11.05 | 11.59 | 7.83 | 10.83 |
| D28 | 11.84 | 12.56 | 10.12 | 11.52 | 11.77 | 12.36 | 13.02 | 8.75 | 12.25 |
| D35 | 12.25 | 13.24 | 10.72 | 12.01 | 12.98 | 13.92 | 14.23 | 9.97 | 15.49 |
| D42 | 14.00 | 15.12 | 11.98 | 13.42 | 14.16 | 15.38 | 16.21 | 12.71 | 17.32 |
| D49 | 16.87 | 17.27 | 13.62 | 15.96 | 16.13 | 17.56 | 19.92 | 14.08 | 20.10 |
TABLE 2 Effect of different treatments on lettuce leaf count (number of slices)
TABLE 3 different treatments for lettuce leaf area (cm)2) Influence of (2)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | CK1 | CK2 | |
| D21 | 1.39 | 1.45 | 0.65 | 1.33 | 1.65 | 2.01 | 2.32 | 0.56 | 2.33 |
| D28 | 9.26 | 10.18 | 2.17 | 9.38 | 9.76 | 10.34 | 11.10 | 2.12 | 15.65 |
| D35 | 14.23 | 15.26 | 4.98 | 14.98 | 15.38 | 15.92 | 16.21 | 5.37 | 22.17 |
TABLE 4 Effect of different treatments on lettuce Dry weight (g)
From the above results, it can be seen that comparative examples 1-2 have certain growth promoting effects on plant height, leaf number, leaf area and dry weight compared with CK1, while comparative example 3 has a reduced plant height, leaf area and dry weight compared with the negative control except that leaf number is not significantly reduced, indicating that the leaching solution of the asterias can inhibit plant growth; after being mixed two by two, comparative examples 4 to 6 have a certain growth promoting effect as compared with CK1, but the growth promoting effect of the treatment with the mixture of the chrysanthemum indicum is weakened; the growth promoting effect of the mixture of the three components is most obvious in the embodiment 1, and compared with CK1, the growth promoting agent has a significant difference and shows a significant synergistic effect of the three components. Compared with CK1, the plant height of example 1 is 41.5%, the leaf number is increased by 24.0%, the leaf area is increased by 201.9%, the dry weight of the aerial part is increased by 75.2%, and the total dry weight is increased by 70.4%. The growth promoting effect of the growth promoter of example 1 is close to that of the positive control with fertilizer, and in actual production, the growth promoter can replace the conventional chemical fertilizer, thereby reducing the application of nitrogen.
Test example 2 potting test
The test adopts a matrix potting method. According to the sand: preparing a mixed substrate by 1:1 vermiculite, equivalently filling the mixed substrate into a plastic flowerpot with the diameter of 15cm, irrigating equivalent tap water to ensure that the substrate is sufficiently wet, sowing 20 lettuce seeds in each pot, and placing the pots in a greenhouse for culturing. After the seeds germinate, the seedlings are thinned when the plant height reaches 2cm, and 5 seedlings are left in each pot. Treatment groups 1 to 7 were prepared by pouring 30ml of the aqueous extract into each pot, followed by the plant growth promoter prepared in example 1 (group 7) and the filtrates prepared in comparative examples 1 to 6 (groups 1 to 6). The negative control was watered with an equal amount of tap water and all treatments were 7 days/1 time. Conventional fertilization was also set as a positive control. All treatments were repeated for 3 pots. Data are collected before each irrigation and are recorded in tables 5-8. And (5) performing data analysis after all data are acquired, and drying and weighing the test material.
TABLE 5 Effect of different treatments on lettuce plant height (cm)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | CK1 | CK2 | |
| D0 | 2.25 | 2.35 | 2.21 | 2.20 | 2.34 | 2.31 | 2.19 | 2.20 | 2.30 |
| D7 | 4.12 | 4.79 | 3.95 | 4.00 | 4.56 | 4.79 | 5.38 | 3.77 | 5.29 |
| D14 | 6.89 | 7.58 | 5.45 | 5.69 | 6.22 | 6.58 | 7.99 | 5.08 | 7.57 |
| D21 | 8.05 | 9.17 | 6.86 | 7.38 | 7.83 | 9.03 | 10.55 | 6.73 | 10.83 |
| D28 | 9.13 | 10.75 | 7.89 | 8.54 | 9.54 | 10.87 | 11.91 | 8.05 | 12.25 |
| D35 | 10.99 | 11.88 | 9.21 | 10.10 | 11.05 | 12.21 | 13.05 | 9.64 | 12.56 |
| D42 | 12.77 | 13.90 | 10.98 | 11.75 | 12.80 | 13.44 | 14.75 | 11.34 | 13.52 |
| D49 | 14.21 | 15.11 | 11.56 | 13.45 | 14.72 | 15.05 | 16.10 | 12.98 | 15.77 |
TABLE 6 influence of different treatments on the number of lettuce leaves (slices)
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | CK1 | CK2 | |
| D0 | |||||||||
| D7 | 1.73 | 1.87 | 1.60 | 1.67 | 1.73 | 1.87 | 2.00 | 1.47 | 1.87 |
| D14 | 2.27 | 2.33 | 2.00 | 2.20 | 2.27 | 2.4 | 2.53 | 2.07 | 2.13 |
| D21 | 3.00 | 3.33 | 2.80 | 2.80 | 3.20 | 3.47 | 3.60 | 2.53 | 3.60 |
| D28 | 3.53 | 3.67 | 3.47 | 3.47 | 3.73 | 3.87 | 4.00 | 3.27 | 3.60 |
| D35 | 4.67 | 4.87 | 4.47 | 4.60 | 4.87 | 5.00 | 5.33 | 4.00 | 4.60 |
| D42 | 5.40 | 5.67 | 5.00 | 5.33 | 5.33 | 5.47 | 5.87 | 4.87 | 6.20 |
| D49 | 5.40 | 5.67 | 5.00 | 5.33 | 5.33 | 5.47 | 5.87 | 5.00 | 6.20 |
TABLE 7 different treatments for lettuce leaf area (cm)2) Influence of (2)
TABLE 8 Effect of different treatments on lettuce Dry weight (g)
The result of the pot experiment is basically consistent with that of the field experiment. The chrysanthemum extract of comparative example 3 has a certain inhibition effect on lettuce growth, while the growth promoter of example 1 shows a remarkable synergistic effect among three components, can remarkably promote lettuce growth, has a similar effect compared with a chemical fertilizer control group, and can replace conventional chemical fertilization in actual production, thereby reducing the application of nitrogen.
Test example 3 field test
In practical application, several leaf vegetables such as pakchoi, lettuce, rape, leaf lettuce, leaf mustard, leaf spinach and the like are selected, soil preparation and sowing are carried out in a field according to a conventional mode, when seeds germinate and the plant height reaches 2cm, the plant growth promoting agent prepared in the embodiment 1-6 is irrigated by 2.5L per square meter to be set as a treatment group 1-6, and equal amount of tap water is irrigated by negative control CK1 for 7 days/1 time. And a fertilizer is used as a positive control CK 2. The material was oven dried and weighed at the time of leaf harvest and the data is reported in tables 9-14.
TABLE 9 Effect of different treatments on the Dry weight (g) of Brassica campestris
TABLE 10 Effect of different treatments on lettuce Dry weight (g)
TABLE 11 Effect of different treatments on the Dry weight (g) of oilseed rape
TABLE 12 Effect of different treatments on the Dry weight (g) of leaf lettuce
TABLE 13 Effect of different treatments on Dry weight (g) of Zanthoxylum schinifolium
TABLE 14 Effect of different treatments on spinach Dry weight (g)
From the above results, it can be seen that the growth promoter of the present invention can significantly increase the dry matter of plants, wherein, under the treatment of example 2, the dry weight and the total dry weight of the above-ground several leaf vegetables such as pakchoi, lettuce, rape, leaf lettuce and spinach are the largest, and the growth promoting effect is the most significant.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the technical principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (6)
1. A plant growth promoter, characterized in that it comprises an aqueous extract of dried powder of aerial parts of chamomile, hyssop and tanacetum parthenium, wherein said aqueous extract is prepared as follows: collecting aerial parts of German chamomile, hyssop and tanacetum vulgare, drying, grinding into powder, extracting in water according to the weight ratio of 1:2-50, standing and soaking at 20-30 ℃ for 24-48h, and taking filtrate, wherein the mass ratio of the German chamomile, the hyssop and the tanacetum vulgare is 1-5:1-5: 1-5.
2. The plant growth promoter according to claim 1, wherein the powder is extracted by being placed in water at a weight ratio of 1:5 to 20.
3. The method for preparing a plant growth promoter according to claim 1, wherein the aerial parts of german chamomile, hyssop and tanacetum parthenium are collected, dried, ground into powder, extracted in water at a weight ratio of 1:2-50, left to stand and soaked at 20-30 ℃ for 24-48h, and the filtrate is taken, wherein the mass ratio of the german chamomile, the hyssop and the tanacetum parthenium dried powder is 1-5:1-5: 1-5.
4. The method for producing a plant growth promoter according to claim 3, wherein the powder is extracted in water at a weight ratio of 1:5 to 20.
5. A method for cultivating a plant, characterized in that the plant growth promoter according to claim 1 or 2 is applied once a week at an application rate of 2.5 liters per square meter when the plant height reaches 2cm or more after the germination of plant seeds.
6. The method of growing a plant according to claim 5, wherein the plant is a vegetable.
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| CN112159268B (en) * | 2020-09-22 | 2022-07-01 | 中国科学院植物研究所 | Pelleting composition and pelleting method for German chamomile seeds |
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| CN102924184A (en) * | 2012-10-11 | 2013-02-13 | 安徽三星化工有限责任公司 | Growth-promoting and insect-killing slow release fertilizer and preparation method thereof |
| CN105085084A (en) * | 2015-09-18 | 2015-11-25 | 王素敏 | Preparation method of nutritious fertilizer capable of promoting root growth |
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| CN105085084A (en) * | 2015-09-18 | 2015-11-25 | 王素敏 | Preparation method of nutritious fertilizer capable of promoting root growth |
| CN105198605A (en) * | 2015-09-29 | 2015-12-30 | 宁国市汉唐盛世农林开发有限公司 | Preparation method of growth-promoting pest-killing additive for special fertilizers for greenhouse vegetables |
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