CN108066501A - A kind of Lignum Aquilariae Resinatum extract and its application with effect of lowering blood sugar - Google Patents
A kind of Lignum Aquilariae Resinatum extract and its application with effect of lowering blood sugar Download PDFInfo
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- lignum aquilariae
- aquilariae resinatum
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- 239000000284 extract Substances 0.000 title claims abstract description 73
- 239000008280 blood Substances 0.000 title claims abstract description 37
- 210000004369 blood Anatomy 0.000 title claims abstract description 37
- 230000000694 effects Effects 0.000 title claims abstract description 31
- 238000000605 extraction Methods 0.000 claims abstract description 53
- 241000271309 Aquilaria crassna Species 0.000 claims abstract description 40
- 238000005422 blasting Methods 0.000 claims abstract description 30
- 239000004382 Amylase Substances 0.000 claims abstract description 13
- 102000013142 Amylases Human genes 0.000 claims abstract description 13
- 108010065511 Amylases Proteins 0.000 claims abstract description 13
- 235000019418 amylase Nutrition 0.000 claims abstract description 13
- 238000007654 immersion Methods 0.000 claims abstract description 11
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 10
- 239000003960 organic solvent Substances 0.000 claims abstract description 10
- 238000010992 reflux Methods 0.000 claims abstract description 10
- 108010059892 Cellulase Proteins 0.000 claims abstract description 9
- 229940106157 cellulase Drugs 0.000 claims abstract description 9
- 238000000034 method Methods 0.000 claims abstract description 8
- 239000012141 concentrate Substances 0.000 claims abstract description 6
- 238000001035 drying Methods 0.000 claims abstract description 6
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 9
- 108010084185 Cellulases Proteins 0.000 claims description 7
- 102000005575 Cellulases Human genes 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 7
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 6
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 6
- 239000003472 antidiabetic agent Substances 0.000 claims description 6
- 238000012545 processing Methods 0.000 claims description 5
- 238000002791 soaking Methods 0.000 claims description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Natural products CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 4
- 230000036541 health Effects 0.000 claims description 4
- 235000019441 ethanol Nutrition 0.000 claims description 3
- 125000005909 ethyl alcohol group Chemical group 0.000 claims description 2
- 230000002218 hypoglycaemic effect Effects 0.000 abstract description 18
- 239000004480 active ingredient Substances 0.000 abstract description 5
- 230000000052 comparative effect Effects 0.000 description 19
- 241001465754 Metazoa Species 0.000 description 9
- 201000001421 hyperglycemia Diseases 0.000 description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 6
- 239000008103 glucose Substances 0.000 description 6
- 239000000243 solution Substances 0.000 description 5
- 241000700159 Rattus Species 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 239000006187 pill Substances 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 206010012601 diabetes mellitus Diseases 0.000 description 3
- 229940088598 enzyme Drugs 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 229920002472 Starch Polymers 0.000 description 2
- 239000002775 capsule Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000002496 gastric effect Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000001802 infusion Methods 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 241001533085 Aquilaria sinensis Species 0.000 description 1
- 241001247437 Cerbera odollam Species 0.000 description 1
- 241001534930 Thymelaeaceae Species 0.000 description 1
- 210000000683 abdominal cavity Anatomy 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 210000001508 eye Anatomy 0.000 description 1
- 210000002683 foot Anatomy 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000035922 thirst Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
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- -1 vina Substances 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/83—Thymelaeaceae (Mezereum family), e.g. leatherwood or false ohelo
- A61K36/835—Aquilaria
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/31—Extraction of the material involving untreated material, e.g. fruit juice or sap obtained from fresh plants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
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Abstract
The invention discloses a kind of Lignum Aquilariae Resinatum extract with effect of lowering blood sugar and its application, the Lignum Aquilariae Resinatum extract is made by method comprising the following steps：By agalloch eaglewood stripping and slicing, it is placed in impregnating in saline solution；Agalloch eaglewood after immersion is subjected to steam blasting；It is handled afterwards using amylase and cellulase；After cleaned, dry, crush, and refluxing extraction is carried out using organic solvent, repeats extraction 2~4 times, merge extracting solution, concentrate drying obtains medicinal extract；Medicinal extract is subjected to CO2Supercritical extract obtains the Lignum Aquilariae Resinatum extract with effect of lowering blood sugar.The present invention extracts each step and mutually acts synergistically so that hypoglycemic active ingredient is fully extracted in agalloch eaglewood, obtains the efficiently hypoglycemic Lignum Aquilariae Resinatum extract with efficient effect of lowering blood sugar.
Description
Technical field
The present invention relates to agalloch eaglewood technical field more particularly to a kind of Lignum Aquilariae Resinatum extracts with effect of lowering blood sugar and its should
With.
Background technology
Agalloch eaglewood is the xylem for containing black resin for Isolated From Thymelaeaceae Species agalloch eaglewood or suspension culture of Aquilaria sinensis.The former main product India and Malaysia
The ground such as West Asia, China are called import agalloch eaglewood, the provinces and regions such as the latter's main product China Hainan, Guangdong and Guangxi, be known as Home-made occluder or
Buta-buta.Agalloch eaglewood is not only traditional rare medicinal herbs of China, Japan, India and other countries in Southeast Asia, is even more a kind of rare
Natural perfume material.Agalloch eaglewood is in the Middle East, China and the Japan history of at least 3000, in India and France or even in the Holy Bible
Also there is the record of agalloch eaglewood in Old Testament.Agalloch eaglewood is still one of fragrance of most expensive in the world at present, and only Singapore goes out every year
The value of mouth agalloch eaglewood is just more than 1,200,000,000 dollars.
Hypoglycemic is to be directed to some hypoglycemic measures that the patient with hyperglycaemia takes.Hyperglycaemia, which refers in blood, to be had
More glucose, beyond normal range (NR), hyperglycaemia is also referred to as hyperglycemia.Normal blood glucose is 70-100.Suffer from hyperglycaemia for a long time
Eyes, kidney, blood vessel, heart, nerve and foot may be damaged.And existing hypoglycemic Western medicine hypoglycemic is fast but simply temporary,
For a long time with liver kidney is damaged, Traditional Chinese medicine for reducing blood glucose can effect a permanent cure, and recover viscera function, and so as to reach the purpose of rehabilitation, but Chinese medicine hypoglycemic is slow.
To sum up, the present invention extracts agalloch eaglewood active ingredient intentionally, can be efficiently hypoglycemic.
The content of the invention
In view of this, it is of the invention the present invention provides a kind of Lignum Aquilariae Resinatum extract with effect of lowering blood sugar and its application
Lignum Aquilariae Resinatum extract has significant hypoglycemic effect, and prepared by the Lignum Aquilariae Resinatum extract application of the present invention hypoglycemic drug, is treated
Hyperglycemia total effective rate is excellent.
The technological means that the present invention uses is as follows：A kind of Lignum Aquilariae Resinatum extract with effect of lowering blood sugar, the agalloch eaglewood extraction
Object is made by method comprising the following steps：
S1, immersion：It by agalloch eaglewood stripping and slicing, is placed in impregnating 30~50 minutes in saline solution, soaking temperature is 70~100 DEG C；
S2, steam blasting：By in the agalloch eaglewood input steam blasting tank after immersion, steam blasting, the pressure of steam blasting are carried out
Power is 3~5MPa, and temperature is 100~120 DEG C, 3~5min of dimension pressure；
S3, enzymolysis：When after steam blasting using amylase and small cellulase processing 1~2, per kilogram agalloch eaglewood using 10~
20g amylase and 5~15g cellulases；
S4, extraction：It cleans, dry after enzymolysis, being crushed to 80~100 mesh, refluxing extraction being carried out using organic solvent, in 70
When~80 DEG C of extractions 1~3 are small, extraction 2~4 times is repeated, merges extracting solution, concentrate drying obtains medicinal extract；
S5, second extraction：Medicinal extract is packed into CO2In supercritical extract tank, Xun Huan adds in CO2Liquid is extracted, extraction temperature
25~35 DEG C, 5~15MPa of extracting pressure of degree, when extraction time 1~2 is small, obtains the Lignum Aquilariae Resinatum extract with effect of lowering blood sugar.
Further, in the step S1, the mass concentration of the saline solution is 5~10%.
Further, in the step S2, the pressure of the steam blasting is 4MPa, and temperature is 110 DEG C, dimension pressure 4min.
Further, in the step S3, the usage amount of the amylase and cellulase is：Per kilogram agalloch eaglewood uses
15g amylase and 10g cellulases.
Further, in the step S4, the organic solvent is ethyl alcohol, one kind in ethyl acetate, chloroform, ether.
Further, in the step S4, the condition of the refluxing extraction is：When 75 DEG C of extractions 2 are small, extraction 3 is repeated
It is secondary.
Further, in the step S5, the CO2Supercritical extract condition is：30 DEG C of extraction temperature extracts pressure
10MPa, when extraction time 1.5 is small.
Further, the agalloch eaglewood is wild agalloch eaglewood or artificial agilawood.
A kind of application of the Lignum Aquilariae Resinatum extract with effect of lowering blood sugar, utilizes any one of them Lignum Aquilariae Resinatum extract of the present invention
Prepare hypoglycemic drug or health products.
Compared with prior art, the beneficial effects of the invention are as follows：The present invention impregnates agalloch eaglewood using saline solution high temperature, makes in it
Then by steam blasting, its active ingredient is separated with impurity for portion's structure softening, using the compound enzymatic treatment of scientific matching,
Efficiently removal impurity, by rationally setting organic solvent refluxing extraction condition, is tentatively extracted, surpasses with reference to excellent CO2 and face
Boundary's extraction conditions obtains the Lignum Aquilariae Resinatum extract containing the hypoglycemic active ingredient of high-purity.The Lignum Aquilariae Resinatum extract of the present invention has aobvious
The hypoglycemic effect of work, each step mutually acts synergistically in extracting method of the invention so that in agalloch eaglewood it is hypoglycemic effectively into
Divide and be fully extracted, obtain efficiently hypoglycemic Lignum Aquilariae Resinatum extract.It prepared by the Lignum Aquilariae Resinatum extract application of the present invention hypoglycemic
Drug, treatment hyperglycemia total effective rate are up to 100%.Hypoglycemic drug or guarantor are prepared using the Lignum Aquilariae Resinatum extract of the present invention
Strong product, the effect for the treatment of cycle can be shortened, therapeutic effect is improved, and radical cure can be played.
Specific embodiment
The principles and features of the present invention are described below, and illustrated embodiment is served only for explaining the present invention, is not intended to
Limit the scope of the present invention.
Embodiment 1
A kind of Lignum Aquilariae Resinatum extract with effect of lowering blood sugar, the Lignum Aquilariae Resinatum extract pass through method comprising the following steps system
：
S1, immersion：Wild agalloch eaglewood or artificial agilawood are subjected to stripping and slicing, are placed in mass concentration to be impregnated in 5% saline solution
50 minutes, soaking temperature was 70 DEG C；
S2, steam blasting：By in the agalloch eaglewood input steam blasting tank after immersion, steam blasting, the pressure of steam blasting are carried out
Power is 3MPa, and temperature is 100 DEG C, dimension pressure 5min；
S3, enzymolysis：When after steam blasting using amylase and small cellulase processing 2, per kilogram agalloch eaglewood uses 10g starch
Enzyme and 5g cellulases；
S4, extraction：It cleans, dry after enzymolysis, being crushed to 80 mesh, refluxing extraction is carried out using organic solvent ethyl acetate,
When 70 DEG C of extractions 3 are small, extraction 2 times is repeated, merges extracting solution, concentrate drying obtains medicinal extract；
S5, second extraction：Medicinal extract is packed into CO2In supercritical extract tank, Xun Huan adds in CO2Liquid is extracted, extraction temperature
25 DEG C, extracting pressure 5MPa of degree, when extraction time 2 is small, obtains the Lignum Aquilariae Resinatum extract with effect of lowering blood sugar.
Embodiment 2
A kind of Lignum Aquilariae Resinatum extract with effect of lowering blood sugar, the Lignum Aquilariae Resinatum extract pass through method comprising the following steps system
：
S1, immersion：Wild agalloch eaglewood or artificial agilawood are subjected to stripping and slicing, are placed in mass concentration to be impregnated in 10% saline solution
30 minutes, soaking temperature was 100 DEG C；
S2, steam blasting：By in the agalloch eaglewood input steam blasting tank after immersion, steam blasting, the pressure of steam blasting are carried out
Power is 5MPa, and temperature is 120 DEG C, dimension pressure 3min；
S3, enzymolysis：When after steam blasting using amylase and small cellulase processing 1, per kilogram agalloch eaglewood uses 20g starch
Enzyme and 15g cellulases；
S4, extraction：It cleans, dry after enzymolysis, being crushed to 100 mesh, carrying out reflux using organic solvents, chloroform or ether carries
It takes, when 80 DEG C of extractions 1 are small, repeats extraction 4 times, merge extracting solution, concentrate drying obtains medicinal extract；
S5, second extraction：Medicinal extract is packed into CO2In supercritical extract tank, Xun Huan adds in CO2Liquid is extracted, extraction temperature
35 DEG C, extracting pressure 15MPa of degree, when extraction time 1 is small, obtains the Lignum Aquilariae Resinatum extract with effect of lowering blood sugar.
Embodiment 3
A kind of Lignum Aquilariae Resinatum extract with effect of lowering blood sugar, the Lignum Aquilariae Resinatum extract pass through method comprising the following steps system
：
S1, immersion：Wild agalloch eaglewood or artificial agilawood are subjected to stripping and slicing, are placed in mass concentration to be impregnated in 8% saline solution
40 minutes, soaking temperature was 85 DEG C；
S2, steam blasting：By in the agalloch eaglewood input steam blasting tank after immersion, steam blasting, the pressure of steam blasting are carried out
Power is 4MPa, and temperature is 110 DEG C, dimension pressure 4min；
S3, enzymolysis：When after steam blasting using amylase and small cellulase processing 1.5, per kilogram agalloch eaglewood is formed sediment using 15g
Powder enzyme and 10g cellulases；
S4, extraction：It cleans, dry after enzymolysis, being crushed to 90 mesh, refluxing extraction being carried out using organic solvent ethyl alcohol, in 75
DEG C extraction 2 it is small when, repeat extraction 3 times, merge extracting solution, concentrate drying obtain medicinal extract；
S5, second extraction：Medicinal extract is packed into CO2In supercritical extract tank, Xun Huan adds in CO2Liquid is extracted, extraction temperature
30 DEG C of degree extracts pressure 10MPa, when extraction time 1.5 is small, obtains the Lignum Aquilariae Resinatum extract with effect of lowering blood sugar.
Comparative example 1
Difference lies in the step S1, saline solution replaces with clear water to this comparative example, and is soaked in room temperature with embodiment 3
Bubble.
Comparative example 2
Difference lies in agalloch eaglewood is directly digested this comparative example after impregnating, without steam blasting with embodiment 3.
Comparative example 3
Difference lies in the step S3, per kilogram agalloch eaglewood uses 5g amylase and 20g to this comparative example with embodiment 3
Cellulase.
Comparative example 4
Difference lies in directly carry out CO to this comparative example with embodiment 3 after enzymolysis2Supercritical extract, without organic solvent
Refluxing extraction.
Comparative example 5
This comparative example and embodiment 3 difference lies in, in the step S5,40 DEG C, extracting pressure 20MPa of extraction temperature,
When extraction time 1.5 is small.
First, animal blood glucose test experiments
1st, experimental animal：SD rats, half male and half female, 180~200g of weight.
2nd, experimental method
Modeling：Rat Fast can't help 12~16h of water, be matched somebody with somebody with the sodium citrate buffer solution (refrigeration) of 0.1mol/L, pH4.2
STZ solution (Fresh) is made, the 1 property injection in abdominal cavity detects blood glucose, random blood sugar value is more than 11.1mmol/L in modeling 7d
It is that modeling is successful to get animal pattern.
3rd, animal packet and administration：SD rats are randomly divided into 11 groups, and every group 10, half male and half female is (normal including normal group
Animal), model group (animal pattern), Examples 1 to 3 group (animal pattern) and comparative example 1~5 group (animal pattern), quench one's thirst
Ball group (animal pattern), normal group and model group give distilled water, Examples 1 to 3 group (animal pattern) and comparative example 1~5
Group is respectively by 0.10g/kg gastric infusions, and model+diabetes pill group is by 5 balls/kg (5 balls weight 1.25g) gastric infusion, 1 time/d, continuously
Administration 2 weeks.
Influence of the table 1 to each group rat blood sugar
| Group | Blood glucose (mmol/L, x ± s) |
| Normal group | 4.98±0.52 |
| Model group | 25.32±0.61* |
| Embodiment 1 | 5.50±0.70 |
| Embodiment 2 | 5.60±0.40 |
| Embodiment 3 | 5.10±0.30 |
| Comparative example 1 | 12.30±1.50 |
| Comparative example 2 | 15.50±1.70 |
| Comparative example 3 | 11.20±1.10 |
| Comparative example 4 | 13.10±1.40 |
| Comparative example 5 | 11.80±1.20 |
| Diabetes pill group | 17.40±1.00# |
Note：Compared with normal group, * P ＜ 0.01；Compared with model group, #P ＜ 0.05.
It is above-mentioned the experimental results showed that, the embodiment of the present invention 1~3 have significant hypoglycemic effect.Wherein, embodiment 3
Hypoglycemic effect is optimal.Comparative example 1~5 is compared with embodiment 3, and the hypoglycemic effect phase of Lignum Aquilariae Resinatum extract is made in comparative example 1~5
To weaker, show that each step mutually acts synergistically in the method for the present invention so that the hypoglycemic active ingredient fully quilt in agalloch eaglewood
Extraction obtains efficiently hypoglycemic Lignum Aquilariae Resinatum extract.Can by the present invention Lignum Aquilariae Resinatum extract application prepare hypoglycemic drug or
Health products shorten treatment cycle, improve therapeutic effect.
2nd, clinical practice
Using conventional method, oral liquid is made in the Lignum Aquilariae Resinatum extract of the embodiment of the present invention 3, collects 100 hyperglycaemia
Disease patient takes the Lignum Aquilariae Resinatum extract oral liquid 1 of the present invention, takes 2 weeks daily, wherein, 65 recoveries from illness, 32 are effective, 4
Effectively, total effective rate 100%.And it has no toxic side effect.
The Lignum Aquilariae Resinatum extract with effect of lowering blood sugar of the present invention can make medicinal various clinically-acceptable dosage forms, also
Including capsule, tablet, powder, granule, vina, pill, micropill preparation, capsule and pill etc., obtained preparation has significantly drop blood
The effect of sugared.
The Lignum Aquilariae Resinatum extract and preparation of the present invention has function of blood sugar reduction, can be applied to preparing the drug of prevention diabetes
In health products.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
God and any modification, equivalent substitution, improvement and etc. within principle, done, should be included within the scope of protection of the invention.
Claims (9)
1. a kind of Lignum Aquilariae Resinatum extract with effect of lowering blood sugar, which is characterized in that the Lignum Aquilariae Resinatum extract is by including following step
Rapid method is made：
S1, immersion：It by agalloch eaglewood stripping and slicing, is placed in impregnating 30~50 minutes in saline solution, soaking temperature is 70~100 DEG C；
S2, steam blasting：By in the agalloch eaglewood input steam blasting tank after immersion, steam blasting is carried out, the pressure of steam blasting is 3
~5MPa, temperature are 100~120 DEG C, 3~5min of dimension pressure；
S3, enzymolysis：When after steam blasting using amylase and small cellulase processing 1~2, per kilogram agalloch eaglewood uses 10~20g
Amylase and 5~15g cellulases；
S4, extraction：It cleans, dry after enzymolysis, being crushed to 80~100 mesh, refluxing extraction being carried out using organic solvent, in 70~80
DEG C extraction 1~3 it is small when, repeat extraction 2~4 times, merge extracting solution, concentrate drying obtain medicinal extract；
S5, second extraction：Medicinal extract is packed into CO2In supercritical extract tank, Xun Huan adds in CO2Liquid is extracted, extraction temperature 25
~35 DEG C, 5~15MPa of extracting pressure, when extraction time 1~2 is small, obtain the Lignum Aquilariae Resinatum extract with effect of lowering blood sugar.
2. the Lignum Aquilariae Resinatum extract according to claim 1 with effect of lowering blood sugar, which is characterized in that in the step S1,
The mass concentration of the saline solution is 5~10%.
3. the Lignum Aquilariae Resinatum extract according to claim 1 with effect of lowering blood sugar, which is characterized in that in the step S2,
The pressure of the steam blasting is 4MPa, and temperature is 110 DEG C, dimension pressure 4min.
4. the Lignum Aquilariae Resinatum extract according to claim 1 with effect of lowering blood sugar, which is characterized in that in the step S3,
The usage amount of the amylase and cellulase is：Per kilogram agalloch eaglewood uses 15g amylase and 10g cellulases.
5. the Lignum Aquilariae Resinatum extract according to claim 1 with effect of lowering blood sugar, which is characterized in that in the step S4,
The organic solvent is ethyl alcohol, one kind in ethyl acetate, chloroform, ether.
6. the Lignum Aquilariae Resinatum extract according to claim 1 with effect of lowering blood sugar, which is characterized in that in the step S4,
The condition of the refluxing extraction is：When 75 DEG C of extractions 2 are small, extraction 3 times is repeated.
7. the Lignum Aquilariae Resinatum extract according to claim 1 with effect of lowering blood sugar, which is characterized in that in the step S5,
The CO2Supercritical extract condition is：30 DEG C of extraction temperature extracts pressure 10MPa, when extraction time 1.5 is small.
8. the Lignum Aquilariae Resinatum extract according to claim 1 with effect of lowering blood sugar, which is characterized in that the agalloch eaglewood is wild
Agalloch eaglewood or artificial agilawood.
9. a kind of application of the Lignum Aquilariae Resinatum extract with effect of lowering blood sugar, which is characterized in that utilize any one of claim 1~8
The Lignum Aquilariae Resinatum extract prepares hypoglycemic drug or health products.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113648680A (en) * | 2021-08-24 | 2021-11-16 | 海口楠脂香业有限公司 | Preparation method for producing and processing agilawood extract |
| CN116459192A (en) * | 2023-05-19 | 2023-07-21 | 广东兆禧健康产业投资有限公司 | Preparation method of aquilaria sinensis extract and toothpaste |
| CN117837742A (en) * | 2024-01-10 | 2024-04-09 | 中国海洋大学 | A method for preparing flavoring sauce from lignum Aquilariae Resinatum extract and diced meat |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102133247A (en) * | 2011-01-28 | 2011-07-27 | 西南大学 | Costustoot extractive with blood sugar reduction activity and application thereof |
| CN105168142A (en) * | 2015-09-30 | 2015-12-23 | 河南行知专利服务有限公司 | Preparation method and application of stem type traditional Chinese medicinal material granules |
-
2017
- 2017-09-06 CN CN201710797738.9A patent/CN108066501B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102133247A (en) * | 2011-01-28 | 2011-07-27 | 西南大学 | Costustoot extractive with blood sugar reduction activity and application thereof |
| CN105168142A (en) * | 2015-09-30 | 2015-12-23 | 河南行知专利服务有限公司 | Preparation method and application of stem type traditional Chinese medicinal material granules |
Non-Patent Citations (5)
| Title |
|---|
| 李响，等: "超临界二氧化碳萃取沉香精油的工艺优化", 《食品工业科技》 * |
| 梅全喜等: "沉香叶与沉香药材降血糖作用的比较研究", 《时珍国医国药》 * |
| 王灿红等: "沉香药用记载、临床应用及药理作用研究进展 ", 《中国药理学与毒理学杂志》 * |
| 邓红梅等: "超临界二氧化碳萃取沉香精油工艺条件研究 ", 《时珍国医国药》 * |
| 郭珍等: "正交试验法优选沙枣花精油提取条件", 《中国调味品》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113648680A (en) * | 2021-08-24 | 2021-11-16 | 海口楠脂香业有限公司 | Preparation method for producing and processing agilawood extract |
| CN116459192A (en) * | 2023-05-19 | 2023-07-21 | 广东兆禧健康产业投资有限公司 | Preparation method of aquilaria sinensis extract and toothpaste |
| CN116459192B (en) * | 2023-05-19 | 2023-09-22 | 广东兆禧健康产业投资有限公司 | Preparation method of aquilaria sinensis extract and toothpaste |
| CN117837742A (en) * | 2024-01-10 | 2024-04-09 | 中国海洋大学 | A method for preparing flavoring sauce from lignum Aquilariae Resinatum extract and diced meat |
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