CN108065342A - The preparation method of collybia albuminosa nutrient solution - Google Patents
The preparation method of collybia albuminosa nutrient solution Download PDFInfo
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- CN108065342A CN108065342A CN201810094819.7A CN201810094819A CN108065342A CN 108065342 A CN108065342 A CN 108065342A CN 201810094819 A CN201810094819 A CN 201810094819A CN 108065342 A CN108065342 A CN 108065342A
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- collybia albuminosa
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- 241000222680 Collybia Species 0.000 title claims abstract description 58
- 235000015097 nutrients Nutrition 0.000 title claims abstract description 40
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 239000004365 Protease Substances 0.000 claims abstract description 27
- 239000007788 liquid Substances 0.000 claims abstract description 22
- 108091005804 Peptidases Proteins 0.000 claims abstract description 21
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000000843 powder Substances 0.000 claims abstract description 15
- 238000001035 drying Methods 0.000 claims abstract description 10
- 239000012153 distilled water Substances 0.000 claims abstract description 9
- 230000007062 hydrolysis Effects 0.000 claims abstract description 7
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 7
- 238000009835 boiling Methods 0.000 claims abstract description 6
- 238000003756 stirring Methods 0.000 claims abstract description 6
- 239000006228 supernatant Substances 0.000 claims abstract description 6
- 230000009514 concussion Effects 0.000 claims abstract description 5
- 238000005485 electric heating Methods 0.000 claims abstract description 4
- 238000007664 blowing Methods 0.000 claims abstract description 3
- 238000000034 method Methods 0.000 claims description 17
- 235000019419 proteases Nutrition 0.000 claims description 17
- 241000287828 Gallus gallus Species 0.000 claims description 7
- 230000001580 bacterial effect Effects 0.000 claims description 6
- 230000003301 hydrolyzing effect Effects 0.000 claims description 6
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 claims description 4
- 239000003513 alkali Substances 0.000 claims description 4
- 235000019834 papain Nutrition 0.000 claims description 4
- 108010004032 Bromelains Proteins 0.000 claims description 3
- 108090000526 Papain Proteins 0.000 claims description 3
- 235000019835 bromelain Nutrition 0.000 claims description 3
- 229940055729 papain Drugs 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 abstract description 14
- 108090000623 proteins and genes Proteins 0.000 abstract description 14
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- 238000000605 extraction Methods 0.000 abstract description 6
- 150000004676 glycans Chemical class 0.000 abstract description 6
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- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 description 8
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- 241000221198 Basidiomycota Species 0.000 description 2
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- 235000013334 alcoholic beverage Nutrition 0.000 description 2
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
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- 229910052737 gold Inorganic materials 0.000 description 2
- PSFDQSOCUJVVGF-UHFFFAOYSA-N harman Chemical compound C12=CC=CC=C2NC2=C1C=CN=C2C PSFDQSOCUJVVGF-UHFFFAOYSA-N 0.000 description 2
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- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical class NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
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- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000006701 autoxidation reaction Methods 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 239000004305 biphenyl Substances 0.000 description 1
- 235000010290 biphenyl Nutrition 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
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- 238000004945 emulsification Methods 0.000 description 1
- 230000001804 emulsifying effect Effects 0.000 description 1
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- 230000002255 enzymatic effect Effects 0.000 description 1
- FPVGTPBMTFTMRT-NSKUCRDLSA-L fast yellow Chemical compound [Na+].[Na+].C1=C(S([O-])(=O)=O)C(N)=CC=C1\N=N\C1=CC=C(S([O-])(=O)=O)C=C1 FPVGTPBMTFTMRT-NSKUCRDLSA-L 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002213 flavones Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
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- 230000003859 lipid peroxidation Effects 0.000 description 1
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- 229910021645 metal ion Inorganic materials 0.000 description 1
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 1
- 239000003595 mist Substances 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000004792 oxidative damage Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- ZUOUZKKEUPVFJK-UHFFFAOYSA-N phenylbenzene Natural products C1=CC=CC=C1C1=CC=CC=C1 ZUOUZKKEUPVFJK-UHFFFAOYSA-N 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- USHAGKDGDHPEEY-UHFFFAOYSA-L potassium persulfate Chemical class [K+].[K+].[O-]S(=O)(=O)OOS([O-])(=O)=O USHAGKDGDHPEEY-UHFFFAOYSA-L 0.000 description 1
- 235000019394 potassium persulphate Nutrition 0.000 description 1
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- 210000002784 stomach Anatomy 0.000 description 1
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- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
- 210000001541 thymus gland Anatomy 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The preparation method of collybia albuminosa nutrient solution of the present invention, belongs to Edible mushroom processing technical field, comprises the following steps：Fresh collybia albuminosa using electric heating constant-temperature blowing drying box in drying, cross 60 mesh sieves and obtain collybia albuminosa powder by milling；Collybia albuminosa powder is weighed, distilled water is added in, stirs to get feed liquid；Above-mentioned feed liquid is heated, adjusts pH, adds in protease, concussion 1.5 2.5h of hydrolysis obtain hydrolyzate；Above-mentioned hydrolyzate is inactivated into 10min in boiling water bath, finally centrifuges 30min, it is collybia albuminosa nutrient solution to take supernatant.The present invention digests the protein in collybia albuminosa using protease, promote cell free by digesting the intercellular protein of collybia albuminosa, with reference to broken slurrying and the enzymolysis of protease, the Polyose extraction in collybia albuminosa liquid is improved, promotes the extraction and hydrolysis of protein；Gained collybia albuminosa nutrient solution integrates polysaccharide, protein, antioxidant peptide active substance, can be made into the functional products such as beverage, oral liquid, capsule or tablet, fermented beverage.
Description
Technical field
The invention belongs to Edible mushroom processing technical fields, and in particular to a kind of preparation method of collybia albuminosa nutrient solution.
Background technology
Collybia albuminosa (Termitomycesalbuminosus) also known as shredded chicken mushroom, ant fir etc. belong to Basidiomycetes
(Basidiomycetes) Agaricales (Agaricales) Tricholomataceae (Tricholomataceae) collybia albuminosa category
(Termitomyces), how open and flat canopy is, and surface is smooth, and early period is in taupe, and for mid-term in cream color, the later stage is in canescence, few
Number bacterium has radial tear；It is mostly deposited with symbiosis of termite, is distributed mainly on the subtropical zone in South Pacific Ocean island and Asia, sub-,
The non-torrid zone, in China, main product is in the Fujian of the southeast, the ground such as Yunnan-Guizhou river in Yunnan and Taiwan.Collybia albuminosa is full of nutrition, and taste is fresh
U.S., be known as mountain delicacy most, the hat laudatory title in seedling, beneficial to stomach, treat hemorrhoid hemostasis, control hemorrhoid and other effects.Collybia albuminosa contains needed by human body ammonia
The nutritional ingredients such as base acid, protein, vitamin, calcium, phosphorus, core yellow acid and polyphenol, cellulase, brain glycosides, polysaccharide isoreactivity ingredient,
It is one of selection that people select supplement needed by human body substance.Bioactive ingredients polyphenol, polysaccharide, flavones in collybia albuminosa etc. are normal
It is used in and repairs affected organ, the adjusting for improving immunity, anti-inflammatory analgesic and physical function etc..
Since free-radical theory is proposed by Harman, many diseases of people and oneself of aging and the generation of human body internal oxidition
It is associated by base and gradually understood by people.Research finds that superfluous free radical can destroy the function and some macromolecular substances of body
Structure such as：Protein, nucleic acid, lipid etc. cause tissue and body cell serious oxidative damage, and then trigger and such as close
The generation of the diseases such as inflammation, atherosclerosis, senile dementia, diabetes, cancer is saved, causes the weight of the substances oxidation deterioration such as grease
Reason is wanted to have free radical, and the variation of the organoleptic qualities such as texture of food, flavor, color and luster is most likely due to free radical and causes
's.These variations result even in the generation of poisonous and harmful substance, and then cause food apoilage and can not eat, therefore grind
Antioxidant is studied carefully with very grave meaning.Protein has antioxidation activity in vivo as macromolecular substances, because it is good
Emulsifying property, emulsification can be played in water-oil interface, therefore the generation of membrane lipid peroxidatio phenomenon can be inhibited, for
Interior free yl is removed to be of great significance.
In recent years, antioxidant is swept across rapidly both at home and abroad, and purposes is also more and more, more and more extensively.Not causing danger property mistake
Quick reaction, it is simple in structure, the characteristics of keeping stablizing, being anti-oxidation peptide under different conditions, the nutrition of itself and functional characteristic
It is even more important.Substantial amounts of research also indicates that, removes free radical, and it is that people are total to inhibit chelated metal ions and lipid peroxidation attribute
The activity of the anti-oxidation peptide of knot.Anti-oxidation peptide protects human tissue organ to be formed from free radical infringement because playing an important role of at present
To study a kind of more biologically active peptide both at home and abroad.By the anti-oxidation peptide of raw material production because its activity is strong, easily absorbs, molecular weight
The features such as small and be concerned, it is very strong to the in vivo active oxygen radical Scavenging activity of machine, the function and body of body can be protected
Normal configuration has broad application prospects in most of fields.Enzyme hydrolysis method is the classical method for preparing anti-oxidation peptide.
Sengupta.R, Anshrullah et al. are respectively with Enzymatic Extraction rice residue protein and rice bran protein, equal display security height.Enzyme process
Hydrolysis is mild with process conditions, and at low cost, Product Safety is good, and low energy consumption, can position the specific peptide of production, not generate and disappear
The advantages that rotation, hydrolytic process is easy to control is currently to produce the most commonly used method of active peptide.
In the prior art, protease hydrolytic is typically that protease is acted on substrate protein white matter, and the protein hydrolyzed is pure
Degree is high, and product is protein hydrolysate micromolecule polypeptide and amino acid, and does not have protease directly acting on collybia albuminosa
Research report.Therefore, to make full use of collybia albuminosa resource, by its main component polysaccharide, protein etc., the enzymolysis of protease is utilized
Effect carries out high efficiency extraction processing, is made more with the polysaccharide, albumen and activity that anti-oxidation function is active and is easily absorbed by the human body
Peptide nutrient solution is of great significance.
The content of the invention
The object of the present invention is to provide the preparation method of collybia albuminosa nutrient solution, to solve the above problems.
To achieve these goals, the technical solution adopted by the present invention is：
The preparation method of collybia albuminosa nutrient solution of the present invention, comprises the following steps：
1) preparation of collybia albuminosa powder：Fresh collybia albuminosa, with electric heating constant-temperature blowing drying box in 55 DEG C of drying after adopting, with crushing
Machine is milled, and crosses 60 mesh sieves and obtains collybia albuminosa powder；
2) dispensing：Collybia albuminosa powder is weighed, distilled water is added in, stirs to get feed liquid；
3) hydrolyze：Above-mentioned feed liquid is heated, adjusts pH, adds in protease, concussion hydrolysis 1.5-2.5h obtains hydrolyzate；
4) post-process：Above-mentioned hydrolyzate is inactivated into 5-20min in boiling water bath, 20-30min is finally centrifuged, takes supernatant i.e.
For collybia albuminosa nutrient solution.
The mass ratio of collybia albuminosa powder and distilled water is 1:(20-33).
The addition of protease is (2500-3500) U/g.
The protease is one or more of alkali protease, neutral proteinase, papain, bromelain
Combination.
Feed liquid is heated to 40-60 DEG C in the step 3) hydrolytic process, pH 5-10.
Feed liquid is heated to 45-55 DEG C in the step 3) hydrolytic process, pH 7-9.
Beneficial effects of the present invention are：The preparation method of collybia albuminosa nutrient solution of the present invention, using protease in collybia albuminosa
Protein digested, protease promotes cell free by digesting the intercellular protein of collybia albuminosa first, with reference to crushing
Machine carries out broken slurrying and the enzymolysis of protease, on the one hand improves the Polyose extraction in collybia albuminosa liquid, also promotes egg
The extraction of white matter；On the other hand, protease has the protein extracted an enzymolysis, and enzymolysis product is peptides, gained chicken fir
Bacterial nutrient solution integrates the main active substances such as polysaccharide, protein, anti-oxidation peptide, the nutrient solution can be made into beverage, oral liquid,
Also alcoholic beverage, acetic acid beverage and lactic acid drink can be prepared through the fermentation of microorganism in capsule and tablet, also can be through spray
Solid beverage, development functionality food is prepared in mist drying.
Description of the drawings
Fig. 1 is Scavenging activity of the nutrient solution to DPPH；
Fig. 2 is nutrient solution to ABTS+Scavenging activity；
Fig. 3 is nutrient solution to O2 -Scavenging activity.
Specific embodiment
Collybia albuminosa, kind are the Termitomyces albuminosus with black skin of domestication, with Intelligent electric heating constant temperature blast drying oven in 55 after adopting
DEG C drying, be milled with pulverizer, cross 60 mesh sieves obtain collybia albuminosa powder, it is spare.
1,1- diphenyl -2- picryls hydrazine (DPPH), Shanghai Jin Sui bio tech ltd；2,2- joins (the 3- second of nitrogen-two
Base-benzothiazole -6- sulfonic acid) di-ammonium salts (ABTS+), Shanghai Jin Sui bio tech ltd；Alkali protease (enzyme activity
100U/mg), Shanghai Jin Sui bio tech ltd；Neutral proteinase (enzyme activity 100U/mg), Shanghai gold fringe biotechnology have
Limit company；Papain (enzyme activity 800U/mg), bromelain (enzyme activity 500U/mg), the limited public affairs of Shanghai gold fringe biotechnology
Department；Pyrogallic acid, three (methylol) aminomethanes, sodium hydroxide, formaldehyde, hydrochloric acid, absolute ethyl alcohol etc., are domestic analysis
It is pure.
TDL-60B low speed desk centrifuges, Anting Scientific Instrument Factory, Shanghai；HH-4 digital display thermostat water baths, Shandong Juancheng
Modern experimental Instrument Ltd.；PHS-3CPH is counted, Shanghai INESA Scientific Instrument Co., Ltd.；723PC spectrophotometers,
Shanghai Lang experimental facilities company；FA2004N electronic balances, Shanghai Precision Scientific Apparatus Co., Ltd；FCD2000 intelligent electrics
Hot constant temperature blast drying oven, Shanghai Lang experimental facilities Co., Ltd.
Embodiment 1
The preparation method of collybia albuminosa nutrient solution, comprises the following steps：
1) dispensing：5g collybia albuminosa powder is weighed, 100g distilled water is added in, stirs to get feed liquid；
2) hydrolyze：Above-mentioned feed liquid is heated to 45 DEG C, adjusts pH to 6.5, adds in 2500U/g papains, shakes water
2.0h is solved, obtains hydrolyzate；
3) post-process：Above-mentioned hydrolyzate is inactivated into 5min in boiling water bath, finally centrifuges 20min, it is chicken fir to take supernatant
Bacterial nutrient solution.
Embodiment 2
The preparation method of collybia albuminosa nutrient solution, comprises the following steps：
1) dispensing：5g collybia albuminosa powder is weighed, 125g distilled water is added in, stirs to get feed liquid；
2) hydrolyze：Above-mentioned feed liquid is heated to 55 DEG C, adjusts pH to 7, adds in 3500U/g alkali proteases, concussion hydrolysis
1.5h obtains hydrolyzate；
3) post-process：Above-mentioned hydrolyzate is inactivated into 10min in boiling water bath, finally centrifuges 30min, it is chicken fir to take supernatant
Bacterial nutrient solution.
Embodiment 3
The preparation method of collybia albuminosa nutrient solution, comprises the following steps：
1) dispensing：5g collybia albuminosa powder is weighed, 150g distilled water is added in, stirs to get feed liquid；
2) hydrolyze：Above-mentioned feed liquid is heated to 50 DEG C, adjusts pH to 9, adds in 3000U/g neutral proteinases, concussion hydrolysis
2.5h obtains hydrolyzate；
3) post-process：Above-mentioned hydrolyzate is inactivated into 20min in boiling water bath, finally centrifuges 25min, it is chicken fir to take supernatant
Bacterial nutrient solution.
The characterization of the antioxidation of nutrient solution：
2 gained collybia albuminosa nutrient solution of Example, is diluted with water to 0.2mg/mL, 0.4mg/mL, 0.6mg/mL, 0.8mg/
ML, 1.0mg/mL, 1.2mg/mL obtain collybia albuminosa nutrient solution dilution, are compared with the Vc under same concentration.
The measure of DPPH clearance rates：
Above-mentioned difference collybia albuminosa nutrient solution dilution 2mL is taken to add in the DPPH of 2mL0.01mmol/L in test tube respectively
Solution is protected from light 30min at room temperature after shaking up, using absolute ethyl alcohol as reference, light absorption value (A is measured in 517nm1).Measure phase
Light absorption value (A after the absolute ethyl alcohol of same volume and DPPH solution mixings2) and 2mL sample solutions and 2mL absolute ethyl alcohol mixings
Light absorption value (A afterwards3), calculate DPPH clearance rates.
The DPPH clearance rates of each sample are measured, are compared with the Vc under same concentration, concrete outcome refers to Fig. 1.
ABTS+The measure of clearance rate：
7mmol/L ABTS are prepared with 2.45mmol/L potassium peroxydisulfates+Storing solution is protected from light and stands 12h.ABTS+Deposit
Liquid dilutes to obtain ABTS with 95% ethyl alcohol+Test fluid, make its 734nm light absorption value be (0.7 ± 0.02).Take respectively it is above-mentioned not
With collybia albuminosa nutrient solution dilution 1mL, 4mLABTS is added in+Test fluid, mixing survey light absorption value (A) measure together after standing 5min
95% ethyl alcohol adds 4mLABTS+Light absorption value (the A of test fluid0), calculate ABTS+Clearance rate.
Measure the ABTS of each sample+Vc under clearance rate, with same concentration is compared, and concrete outcome refers to Fig. 2.
O2 -The measure of clearance rate：
3mmoL/L pyrogallol solution 0.3mL are added in into each volumetric flask, then are added respectively.Above-mentioned different chicken firs are taken respectively
Bacterial nutrient solution dilution 2mL is settled to 9mL with the Tris-HCl buffer solutions of pH8.2, and rapid mixing is simultaneously joined with distilled water work
Than, survey absorbance every 30s at 325nm wavelength, the 9min after reaction starts terminates to measure, the data of gained with
Time is abscissa, and absorbance carries out linear regression curves for ordinate, and obtained straight slope is reaction rate Δ A0, Δ A,
Calculation formula is as follows：
In formula：ΔA0- be pyrogallol Autoxidation Method rate；Δ A-it is to add in mouse thymus cells after nutrient solution
Method rate (unit is absorbance increment per minute).
Measure the O of each sample2 -Vc under clearance rate, with same concentration is compared, and concrete outcome refers to Fig. 3.
Experimental researches prove that collybia albuminosa nutrient solution provided by the invention has very strong antioxidation activity, therefore this hair
The bright collybia albuminosa nutrient solution can be applied to prepare anti-oxidation function type beverage.
Beverage, oral liquid, capsule and piece can be made in collybia albuminosa nutrient solution by collybia albuminosa nutrient solution provided by the invention
Agent also can be prepared alcoholic beverage, acetic acid beverage and lactic acid drink through the fermentation of microorganism, spray-dried can also make
It is standby to obtain solid beverage, development functionality food.
Claims (6)
1. a kind of preparation method of collybia albuminosa nutrient solution, which is characterized in that comprise the following steps：
1) preparation of collybia albuminosa powder：Fresh collybia albuminosa is ground with electric heating constant-temperature blowing drying box after adopting in 55 DEG C of drying with pulverizer
Powder crosses 60 mesh sieves and obtains collybia albuminosa powder；
2) dispensing：Collybia albuminosa powder is weighed, distilled water is added in, stirs to get feed liquid；
3) hydrolyze：Above-mentioned feed liquid is heated, adjusts pH, adds in protease, concussion hydrolysis 1.5-2.5h obtains hydrolyzate；
4) post-process：Above-mentioned hydrolyzate is inactivated into 5-20min in boiling water bath, finally centrifuges 20-30min, it is chicken to take supernatant
Fir bacterial nutrient solution.
2. the preparation method of collybia albuminosa nutrient solution according to claim 1, which is characterized in that collybia albuminosa powder and distilled water
Mass ratio is 1:(20-33).
3. the preparation method of collybia albuminosa nutrient solution according to claim 1, which is characterized in that the addition of protease is
(2500-3500)U/g。
4. the preparation method of the collybia albuminosa nutrient solution according to claim 1 or 3, which is characterized in that the protease is alkali
The combination of one or more of property protease, neutral proteinase, papain, bromelain.
5. the preparation method of collybia albuminosa nutrient solution according to claim 1, which is characterized in that the step 3) hydrolytic process
Middle feed liquid is heated to 40-60 DEG C, pH 5-10.
6. the preparation method of collybia albuminosa nutrient solution according to claim 5, which is characterized in that the step 3) hydrolytic process
Middle feed liquid is heated to 45-55 DEG C, pH 7-9.
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Application publication date: 20180525 |