CN108061799A - A kind of chromatographic test paper strip device - Google Patents
A kind of chromatographic test paper strip device Download PDFInfo
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- CN108061799A CN108061799A CN201711283955.2A CN201711283955A CN108061799A CN 108061799 A CN108061799 A CN 108061799A CN 201711283955 A CN201711283955 A CN 201711283955A CN 108061799 A CN108061799 A CN 108061799A
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- pad
- bottom plate
- pasted
- nitrocellulose filter
- test paper
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
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- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
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- Chemical Kinetics & Catalysis (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a kind of chromatographic test paper strip devices, including pasting bottom plate (4), it is pasted on the nitrocellulose filter (3) of described stickup bottom plate (4) upper surface, it is pasted on the diagnosing pad (6) of described stickup bottom plate (4) first end upper surface, and it is pasted on the blotting paper (5) of described stickup bottom plate (4) second end upper surface, have first to be sized overlap distance between the diagnosing pad (6) and the nitrocellulose filter (3), have second to be sized overlap distance between the blotting paper (5) and the nitrocellulose filter (3), the stickup bottom plate (4), the nitrocellulose filter (3), the diagnosing pad (6) and the blotting paper (5) are of same size, and width is between 1 2.5mm scopes.The chromatographic test paper strip device efficiently solves the problems such as diagnosis chromatograph test strip wider width sample requirement amount is larger.
Description
Technical field
The present invention relates to chromatographic technique field, more particularly to a kind of chromatographic test paper strip device.
Background technology
In the prior art, it is all diagnosis chromatography reagents (lateral chromatography, two-way flow measurement) test strips width 2.5mm with
On, quantitative test paper item is in more than 3mm.Since paper slip is wider, cause it more to the demand of sample, the sample-adding amount of quantitative reagent
In more than 50ul.For it is existing diagnosis chromatograph test strip wider width, cause sample-adding amount excessive, substantially increase material into
This.
In conclusion the problems such as how efficiently solving diagnosis chromatograph test strip wider width, is current art technology
Personnel's urgent problem.
The content of the invention
The object of the present invention is to provide a kind of chromatographic test paper strip device, which efficiently solves diagnosis
The problems such as chromatograph test strip wider width sample requirement amount is excessive.
In order to solve the above technical problems, the present invention provides following technical solution：
A kind of chromatographic test paper strip device, the nitric acid including pasting bottom plate, being pasted in the middle part of the stickup plate upper surface are fine
The plain film of dimension, the diagnosing pad for being pasted on the stickup bottom plate first end upper surface, the diagnosing pad and the nitrocellulose filter it
Between have first to be sized overlap distance, the width phase for pasting bottom plate, the nitrocellulose filter and the diagnosing pad
Together, and width is between 1-2.5mm scopes；Further include the blotting paper for being pasted on the stickup bottom plate second end upper surface.
Preferably, the diagnosing pad for individualism sample pad and conjugate pad or functionally can be two-in-one one kind
Gasket.
Preferably, the diagnosing pad include one end be pasted on the stickup bottom plate first end upper surface conjugate pad and
One end is pasted on the stickup plate upper surface and close to the sample pad of the conjugate pad, the sample pad and the combination
Have the 3rd to be sized overlap distance between object pad.
Preferably, intermediate sample pad is further included, the intermediate sample pad is pasted onto the cellulose nitrate by double faced adhesive tape
The upper surface of plain film.
Preferably, second is sized overlap distance between the blotting paper and the nitrocellulose filter, it is described
Blotting paper and the diagnosing pad be of same size or longitudinal direction intersects placement with the nitrocellulose filter.
Preferably, the blotting paper is single layer water absorption paper or multi-layer absorbent paper.
Chromatographic test paper strip device provided by the present invention, suitable for colloidal gold, collargol, quantum dot, fluorescent microsphere, fluorescence
Color micro-sphere etc. can with coupled antibody, the colour developing object of antigen isoreactivity material, using nitrocellulose filter as solid phase reaction carrier,
With whole blood, blood plasma, serum, urine, saliva is the various detection project chromatograph test strips of sample, including lateral chromatography, two-way survey
The chromatography reagent such as stream.Chromatographic test paper strip device includes pasting bottom plate, nitrocellulose filter, blotting paper and diagnosing pad, blotting paper
It is pasted on and pastes bottom plate second end upper surface, nitrocellulose filter, which is pasted on, pastes upper surface in the middle part of bottom plate, and diagnosing pad is pasted on
The upper surface of bottom plate first end is pasted, has first to be sized overlap distance, nitric acid between diagnosing pad and nitrocellulose filter
The various gaskets such as cellulose membrane, diagnosing pad, which are pasted onto, to be pasted on bottom plate, and it is each to paste bottom plate, nitrocellulose filter, diagnosing pad etc.
Various sizes of overlapping is needed between kind gasket, so that sample chromatographs, specific overlapping dimension is with applying to different size of conjugate
It is related.Being of same size for bottom plate, nitrocellulose filter and diagnosing pad is pasted, and width is between 1-2.5mm scopes.It needs to illustrate
, after previous test strips constriction, edge effect is susceptible to, causes background unclean, directly affects the standard of instrument readings
True property to solve problems, optimizes, and mark colour developing carrier size is screened, such as colloidal gold, microballoon, quantum
Point etc., selects using 30-230nm range vectors and on this basis suitable coupling condition, makes the active material of carrier conjugation
Reach preferable activity, and conjugate gasket is pre-processed with macromoleculars such as albumen, it, can after sample-adding beneficial to the stabilization of conjugate
Quick and complete release conjugate, and complete to chromatograph, it is unlikely to cause to be detained on film.It solves conjugate with this to remain in instead
The background problems such as the edge effect generated in area are answered, break through the width limitation of previous test strips.
Chromatographic test paper strip device provided by the present invention, by the diagnosing pad of pretreatment, nitrocellulose filter, according to different item
Mesh, size needed for different conjugate sizes, which is pasted onto, pastes on bottom plate, is then cut into test paper of the width in 1-2.5mm scopes
Item not only directly significantly saves test strips material cost, but also improves the quantum of output of equal raw material simultaneously, and sample-adding amount is reduced
To 15-50ul.When detecting whole blood sample, directly using a small amount of peripheral blood.
Description of the drawings
It in order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
There is attached drawing needed in technology description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention, for those of ordinary skill in the art, without creative efforts, can be with
Other attached drawings are obtained according to these attached drawings.
The structure diagram for the chromatographic test paper strip device that Fig. 1 is provided for the first specific embodiment in the present invention；
Fig. 2 is the sectional view of Fig. 1；
The structure diagram for the chromatographic test paper strip device that Fig. 3 is provided for second of specific embodiment in the present invention；
Fig. 4 is the sectional view of Fig. 3；
The structure diagram for the chromatographic test paper strip device that Fig. 5 is provided for the third specific embodiment in the present invention；
Fig. 6 is the sectional view of Fig. 5.
It is marked in attached drawing as follows：
1- sample pads, 2- conjugates pad, 3- nitrocellulose filters, 4- are pasted in bottom plate, 5- blotting papers, 6- diagnosing pads, 7-
Between sample pad, 8- double faced adhesive tapes.
Specific embodiment
The core of the present invention is to provide a kind of chromatographic test paper strip device, which efficiently solves diagnosis
The problems such as chromatograph test strip wider width sample requirement amount is larger.
Below in conjunction with the attached drawing in the embodiment of the present invention, the technical solution in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained every other without making creative work
Embodiment belongs to the scope of protection of the invention.
The chromatographic test paper strip device provided to Fig. 6, Fig. 1 for the first specific embodiment in the present invention is be provided
Structure diagram；Fig. 2 is the sectional view of Fig. 1；The chromatographic test paper that Fig. 3 is provided for second of specific embodiment in the present invention
The structure diagram of strip device；Fig. 4 is the sectional view of Fig. 3；The layer that Fig. 5 is provided for the third specific embodiment in the present invention
Analyse the structure diagram of test paper strip device；Fig. 6 is the sectional view of Fig. 5.
In a kind of specific embodiment, chromatographic test paper strip device provided by the present invention, suitable for colloidal gold, colloid
Silver, quantum dot, fluorescent microsphere, fluorescent color microballoon etc. can be fine with nitric acid with coupled antibody, the colour developing object of antigen isoreactivity material
The plain film 3 of dimension is solid phase reaction carrier, and with whole blood, blood plasma, serum, urine, saliva is the various detection projects chromatography examination of sample
Paper slip, including lateral chromatography, the chromatography reagent such as two-way flow measurement.Chromatographic test paper strip device includes pasting bottom plate 4, nitrocellulose filter
3rd, blotting paper 5 and diagnosing pad, blotting paper 5, which is pasted on, pastes 4 second end upper surface of bottom plate, and nitrocellulose filter 3 is pasted on viscous
4 middle part upper surface of bottom plate is pasted, diagnosing pad 6 is pasted on the upper surface for pasting 4 first end of bottom plate, diagnosing pad 6 and nitrocellulose filter 3
Between have first to be sized overlap distance, the various gaskets such as nitrocellulose filter 3, diagnosing pad 6, which are pasted onto, pastes bottom plate 4
On, various sizes of overlapping is needed between the various gaskets such as stickup bottom plate 4, nitrocellulose filter 3, diagnosing pad 6, so as to sample layer
Analysis, specific overlapping dimension are related with applying to different size of conjugate.Paste bottom plate 4, nitrocellulose filter 3, diagnosing pad 6
It is of same size, and width is between 1-2.5mm scopes.It should be noted that after previous test strips constriction, edge is susceptible to
Effect causes background unclean, directly affects the accuracy of instrument readings, to solve problems, optimizes, aobvious to marking
Color carrier size is screened, such as colloidal gold, microballoon, quantum dot, using 30-230nm range vectors and on this basis
Suitable coupling condition is selected, the active material of carrier conjugation is made to reach preferable activity, and is pre-processed with macromoleculars such as albumen
Conjugate pad, beneficial to the stabilization of conjugate, release conjugate that can be quick and complete after sample-adding, and complete to chromatograph, it is unlikely to
It causes to be detained on film.It solves conjugate with this and remains in the background problems such as the edge effect generated in reaction zone, break through
The width limitation of previous test strips.
Chromatographic test paper strip device provided by the present invention, by the diagnosing pad 6 of pretreatment, nitrocellulose filter 3, according to difference
Project, size needed for different conjugate sizes, which is pasted onto, pastes on bottom plate 4, is then cut into examination of the width in 1-2.5mm scopes
Paper slip not only directly significantly saves test strips material cost, improves production efficiency, the assembled big plate of similary length, cutting
The test strips quantity obtained afterwards improves the output of 15-400% than the test strips of previous 3mm-4mm width.And sample-adding amount is reduced
To 15-50ul, when detecting whole blood sample, directly using a small amount of peripheral blood.
Above-mentioned chromatographic test paper strip device is only a kind of preferred embodiment, is specifically not limited thereto, on this basis can basis
Actual needs is made with targetedly adjusting, and so as to obtain different embodiments, diagnosing pad 6 can be independent sample pad 1
And conjugate pad 2, sample pad 1 include processing sample reagent, conjugate pad 2 includes conjugates；It is or functionally two-in-one
Gasket, this gasket includes pretreatment sample reagent and conjugate；Nitrocellulose filter 3 can be coated with 1-2 nature controlling line above
And one to several detection lines, which kind of situation specific diagnosing pad 6 and nitrocellulose filter 3 are, can be according to practical situations
Depending on, all within the scope of the present invention.
On the basis of above-mentioned specific embodiment, those skilled in the art can be according to the difference of specific occasion, to layer
It analyses test paper strip device and carries out several changes, diagnosing pad 6 includes conjugate pad 2 and sample pad 1, and 2 one end of conjugate pad are pasted on viscous
4 first end upper surface of bottom plate is pasted, 1 one end of sample pad, which is pasted on, pastes bottom plate 4 close to the upper surface of conjugate pad paste position, sample
Have the 3rd to be sized overlap distance between this pad 1 and conjugate pad 2, specific overlapping dimension is with applying to different size of idol
It is related to join object, pastes bottom plate 4, nitrocellulose filter 3, conjugate pad 2, sample pad 1 and is of same size, and width is in 1-2.5mm
Between scope, test strips material cost is not only directly significantly saved, while improves the quantum of output of equal raw material, and sample-adding amount is subtracted
As little as 15-50ul.
Obviously, under the guidance of this thought, those skilled in the art can be according to the difference of specific occasion to above-mentioned
Specific embodiment carries out several changes, further includes intermediate sample pad 7, the intermediate sample pad 7 is pasted onto by double faced adhesive tape 8
The upper surface of the nitrocellulose filter 3, lateral chromatography, two-way flow measurement etc. chromatograph reagent, increase intermediate sample pad 7, can be used for
The immune response of distinct methods and different sample loading alternatives.Intermediate sample pad 7 is easy to connect, is easier to implement.It should be noted that
Here and foregoing test paper strip device can be packed into corresponding test gets stuck, and also can individually complete to react, more convenient.
It is important to note that chromatographic test paper strip device provided by the present invention is not intended to be limited to such situation, also
Blotting paper 5 including being pasted on the stickup bottom plate 4 second end upper surface, the blotting paper 5 and the nitrocellulose filter 3 it
Between have and second be sized overlap distance, blotting paper 5 is of same size with diagnosing pad 6, with preferable water imbibition.Blotting paper
Or individually longitudinal direction intersects placement with nitrocellulose filter 3.
Chromatographic test paper strip device provided by the present invention, immovable in other components, blotting paper 5 can be single
Layer blotting paper, it is simple in structure；Can also multi-layer absorbent paper, soaking effect is preferable, and concrete form is unrestricted, can be according to specific
The different sets itselfs of service condition, all within the scope of the present invention.
In the description of the present invention, it is necessary to which the orientation of the instructions such as explanation, term end or position relationship are based on attached drawing
Shown orientation or position relationship, are for only for ease of the description present invention and simplify description rather than instruction or imply meaning
Device or element must have specific orientation, with specific azimuth configuration and operation, therefore it is not intended that the present invention's
Limitation.
Each embodiment is described by the way of progressive in this specification, the highlights of each of the examples are with other
The difference of embodiment, just to refer each other for identical similar portion between each embodiment.
The foregoing description of the disclosed embodiments enables professional and technical personnel in the field to realize or use the present invention.
A variety of modifications of these embodiments will be apparent for those skilled in the art, it is as defined herein
General Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, it is of the invention
The embodiments shown herein is not intended to be limited to, and is to fit to and the principles and novel features disclosed herein phase one
The most wide scope caused.
Claims (6)
1. a kind of chromatographic test paper strip device, which is characterized in that including pasting bottom plate (4), being pasted on described stickup bottom plate (4) upper table
The nitrocellulose filter (3) of Middle face, the diagnosing pad (6) for being pasted on described stickup bottom plate (4) first end upper surface, the diagnosis
Have first to be sized overlap distance, the stickup bottom plate (4), the nitre between pad (6) and the nitrocellulose filter (3)
Acid cellulose film (3) and the diagnosing pad (6) are of same size, and width is between 1-2.5mm scopes；It further includes and is pasted on institute
State the blotting paper (5) for pasting bottom plate (4) second end upper surface.
2. chromatographic test paper strip device according to claim 1, which is characterized in that the diagnosing pad (6) is individualism
Sample pad (1) and conjugate pad (2) or a kind of gasket that functionally can be two-in-one.
3. chromatographic test paper strip device according to claim 1, which is characterized in that the diagnosing pad (6) is pasted including one end
Described stickup bottom plate (4) upper surface is pasted in the conjugate pad (2) of described stickup bottom plate (4) first end upper surface and one end
And close to the sample pad (1) of the conjugate pad (2), have the 3rd to set between the sample pad (1) and the conjugate pad (2)
It is sized overlap distance.
4. chromatographic test paper strip device according to claim 1, which is characterized in that intermediate sample pad (7) is further included, in described
Between sample pad (7) upper surfaces of the nitrocellulose filter (3) is pasted onto by double faced adhesive tape (8).
5. according to claim 1-4 any one of them chromatographic test paper strip devices, which is characterized in that the blotting paper (5) and institute
Stating between nitrocellulose filter (3) has second to be sized overlap distance, the blotting paper (5) and the diagnosing pad (6)
It is of same size or longitudinal direction intersects placement with the nitrocellulose filter (3).
6. chromatographic test paper strip device according to claim 5, which is characterized in that the blotting paper (5) is single layer water absorption paper
Or multi-layer absorbent paper.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711283955.2A CN108061799A (en) | 2017-12-07 | 2017-12-07 | A kind of chromatographic test paper strip device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711283955.2A CN108061799A (en) | 2017-12-07 | 2017-12-07 | A kind of chromatographic test paper strip device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108061799A true CN108061799A (en) | 2018-05-22 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711283955.2A Pending CN108061799A (en) | 2017-12-07 | 2017-12-07 | A kind of chromatographic test paper strip device |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1277357A (en) * | 1999-06-14 | 2000-12-20 | 普莱克斯生物系统股份有限公司 | Two-way sidestream test strip |
| CN101666799A (en) * | 2009-07-23 | 2010-03-10 | 江苏省农业科学院 | Test strip for detecting colibacillus O157:H7 mouse serum antibody by colloidal gold immunochromatography |
| CN104142394A (en) * | 2013-05-10 | 2014-11-12 | 福建省水产研究所 | Making method of semi-quantitative tetrodotoxin detection card |
| WO2016187244A1 (en) * | 2015-05-19 | 2016-11-24 | Ortho-Clinical Diagnostics, Inc. | Method of improving liquid sample flow in assay device |
| CN107064490A (en) * | 2017-05-18 | 2017-08-18 | 中国科学院烟台海岸带研究所 | A kind of utilize recombinates detection reagent bar of fluorescence phycobniliprotein subunit and preparation method thereof |
-
2017
- 2017-12-07 CN CN201711283955.2A patent/CN108061799A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1277357A (en) * | 1999-06-14 | 2000-12-20 | 普莱克斯生物系统股份有限公司 | Two-way sidestream test strip |
| CN101666799A (en) * | 2009-07-23 | 2010-03-10 | 江苏省农业科学院 | Test strip for detecting colibacillus O157:H7 mouse serum antibody by colloidal gold immunochromatography |
| CN104142394A (en) * | 2013-05-10 | 2014-11-12 | 福建省水产研究所 | Making method of semi-quantitative tetrodotoxin detection card |
| WO2016187244A1 (en) * | 2015-05-19 | 2016-11-24 | Ortho-Clinical Diagnostics, Inc. | Method of improving liquid sample flow in assay device |
| CN107064490A (en) * | 2017-05-18 | 2017-08-18 | 中国科学院烟台海岸带研究所 | A kind of utilize recombinates detection reagent bar of fluorescence phycobniliprotein subunit and preparation method thereof |
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Application publication date: 20180522 |