CN108048312A - A kind of use for laboratory nucleic acid extraction operation console - Google Patents
A kind of use for laboratory nucleic acid extraction operation console Download PDFInfo
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- CN108048312A CN108048312A CN201711251468.8A CN201711251468A CN108048312A CN 108048312 A CN108048312 A CN 108048312A CN 201711251468 A CN201711251468 A CN 201711251468A CN 108048312 A CN108048312 A CN 108048312A
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- storage box
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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Abstract
The invention discloses a kind of effective use for laboratory nucleic acid extraction operation consoles for maintaining low-temperature condition and effect is good, at low cost, cooling substance can recycle.It includes water bath, rack for test tube, cylinder, ice bag, storage box and supporting rack, the water bath and cylinder are each provided on supporting rack, cylinder is located at the lower section of water bath, cylinder includes cylinder body and vertically moving piston rod, the bottom of water bath is equipped with sliding sleeve, and storage box is slidably connected with sliding sleeve, and the glide direction of storage box is vertical, ice bag is located in storage box, and storage box is equipped with to access the inlet slot of ice bag；Sealing ring and lower seal are arranged on storage box, inlet slot is between upper sealing ring and lower seal, the height of sliding sleeve is more than the spacing of upper sealing ring and lower seal, test tube is erected in water bath, rack for test tube is located at the top of storage box, rack for test tube is equipped with several test tubes for being used to store nucleic acid raw material, is equipped with to monitor the temperature sensor of water temperature in water bath.
Description
Technical field
The present invention relates to nucleic acid extraction technical field more particularly to a kind of use for laboratory nucleic acid extraction operation consoles.
Background technology
With the development of molecular engineering, the extracting mode of nucleic acid is various, and nucleic acid extraction kit also commercialization.
During extracting nucleic acid, in order to protect nucleic acid, prevent its degradation, whole process is required to be carried out under conditions of low temperature in principle
Operation.Include ice machine in conventional molecule experiments instrument, nucleic acid extraction operating process is caused by ice machine on trash ice
It carries out, whole process is made to maintain a low-temperature condition close to zero degree.But the use cost of ice machine is higher, if experiment
Dosage is few, and generated ice can not preserve, and causes to waste.
203824194 U of Chinese patent Authorization Notice No. CN, authorized announcement date 2014.09.10, entitled " small-sized system
Ice maker ", disclose it is a kind of by main box, casing, control panel, water supply installation, ice making deicing apparatus and shovel ice production apparatus form it is small
Type ice machine, this ice machine can generate suitable trash ice, reduce ice machine use cost, but there are still trash ices by outer
Boundary's temperature influences easily to melt, and trash ice corner angle are clearly demarcated, easily the problems such as scuffing experimenter.
The content of the invention
The purpose of the invention is to overcome deficiency of the prior art, a kind of effectively maintenance low-temperature condition and effect are provided
The use for laboratory nucleic acid extraction operation console that good, at low cost, cooling substance can recycle.
To achieve these goals, the present invention uses following technical scheme：
A kind of use for laboratory nucleic acid extraction operation console, including
Water bath, rack for test tube, cylinder, ice bag, storage box and supporting rack, the water bath and cylinder are each provided on supporting rack, gas
Cylinder is located at the lower section of water bath, and cylinder includes cylinder body and vertically moving piston rod, and the bottom of water bath is equipped with sliding sleeve,
Storage box is slidably connected with sliding sleeve, and the glide direction of storage box is vertical, and ice bag is located in storage box, and storage box is equipped with to deposit
Take the inlet slot of ice bag；
Sealing ring and lower seal are arranged on the storage box, inlet slot is sliding between upper sealing ring and lower seal
The height of set is more than the spacing of upper sealing ring and lower seal, and test tube is erected in water bath, and rack for test tube is located at the upper of storage box
Side, rack for test tube are equipped with several test tubes for being used to store nucleic acid raw material, are equipped with to monitor the temperature sensing of water temperature in water bath
Device.The ice bag freezed is put into storage box, water is injected into water bath, ice bag cools down to water, equipped with nucleic acid raw material
Test tube immerse low temperature water in, temperature sensor record water temperature, cylinder will push up on storage box if water temperature over-high, make ice bag with
Water contacts, and makes water continued down, and cylinder pulls down storage box if water temperature is too low, and ice bag is made to completely cut off with water, prevents water freezing from freezing
Firmly test tube and nucleic acid raw material.
Preferably, support frame as described above includes height adjustment device, height adjustment device includes platform, perpendicular screw rod and support
Plate, support plate are fixed on supporting rack, and support plate is located at the lower section of platform, and cylinder is located on platform, erect the upper end of screw rod with putting down
Platform is rotatablely connected, and perpendicular screw rod is connected with support plate thread, is erected screw rod and is passed through support plate.The stroke of existing cylinder is mostly fixation
, and the height residing for the adjustable displacements of cylinders of height adjustment device, so as to adjust the high-low limit position of storage box, when ice bag is multiple
After refrigeration effect reduces, the height of cylinder can be turned down, when storage box is in lower limit position, inlet slot can expose sliding sleeve, from
And ice bag can be taken out to the ice bag frozen for freezing or more renewing again, the specific adjusting method of cylinder height is：Rotating vertical
Screw rod rises or falls platform, and since the upper end of platform and perpendicular screw rod is rotatablely connected, platform can't be with the rotation of perpendicular screw rod
And it significantly rotates.
Preferably, the height adjustment device further includes two spline structures, spline structure includes setting on the supporting plate
Dog link and the pilot hole that is located on platform, platform be slidably matched with dog link, the glide direction of platform and the axis of dog link
Line is parallel, in a spline structure：Dog link passes through pilot hole.Arresting lever thoroughly limits the rotation of platform, platform can only on
It rises or declines, beneficial to the stability of cylinder operation.
Preferably, the lower end of the perpendicular screw rod is equipped with the regulation handle for rotating vertical screw rod.It is rotated with hand and adjusts hand
Handle can rotating vertical screw rod, it is convenient and efficient.
Preferably, the sidewall horizontal of the water bath extends inwardly to form baffle ring, test tube is erected on baffle ring.Baffle ring is used
In holding test tubes frame, facilitate the rack for test tube for replacing different size, and baffle ring plays the role of marking water level simultaneously, and control is facilitated to need
The water of injection enables test tube lower end to be immersed in the water, and keeps low-temperature condition.
Preferably, the material of the storage box is foamed plastics.Foamed plastics has both that light, adiabatic, water absorption rate is low, anti-
The features such as shaking, is corrosion-resistant is suitble to the working environment of storage box in the present invention.
Preferably, the temperature sensor is located at the lower section of rack for test tube, temperature sensor is close to rack for test tube.Temperature sensing
Device is located proximate to rack for test tube, makes the water temperature measured close to the water temperature at test tube.
The beneficial effects of the invention are as follows：（1）The water in water bath is made to cool down by ice bag, the cryogenic effect of water-bath compares trash ice
It is good, and there is no the security risks that trash ice scratches experimenter；（2）Temperature sensor can measure the temperature of water in water bath,
It is slided up and down by cylinder by storage box, contact or isolation of the control ice bag with water, to prevent water temperature over-high from causing the failure of an experiment
Or water temperature is too low causes to freeze；（3）It can be recycled after ice bag frost, it is at low cost, and ice bag high cooling efficiency, it is easy to use.
Description of the drawings
Fig. 1 is the structure diagram of the present invention；
Fig. 2 is the enlarged drawing at A in Fig. 1.
In figure：Water bath 1, sliding sleeve 11, temperature sensor 12, baffle ring 13, rack for test tube 2, test tube 21, cylinder 3, cylinder body 31,
Piston rod 32, ice bag 4, storage box 5, inlet slot 51, upper sealing ring 52, lower seal 53, supporting rack 6, platform 61, perpendicular screw rod
62nd, support plate 63, dog link 64, regulation handle 65.
Specific embodiment
The present invention will be further described in the following with reference to the drawings and specific embodiments.
As shown in Fig. 1 to Fig. 2, a kind of use for laboratory nucleic acid extraction operation console, including
Water bath 1, rack for test tube 2, cylinder 3, ice bag 4, storage box 5 and supporting rack 6, the water bath and cylinder are each provided at supporting rack
On, cylinder is located at the lower section of water bath, and cylinder includes cylinder body 31 and vertically moving piston rod 32, the bottom of water bath
Equipped with sliding sleeve 11, storage box is slidably connected with sliding sleeve, and the glide direction of storage box is vertical, and ice bag is located in storage box, storage box
It is equipped with to access the inlet slot 51 of ice bag；
Be arranged with sealing ring 52 and lower seal 53 on the storage box, inlet slot be located at upper sealing ring and lower seal it
Between, the height of sliding sleeve is more than the spacing of upper sealing ring and lower seal, and test tube is erected in water bath, and rack for test tube is located at storage box
Top, rack for test tube is equipped with several for storing the test tubes 21 of nucleic acid raw material, is equipped with to monitor the temperature of water temperature in water bath
Spend sensor 12.
Ice bag is a kind of novel refrigerant, and when thaw does not have water pollution, can Reusability, effectively use
Cold capacity is 6 times of androgynous accumulated ice, and ice bag in -20 DEG C is freezed, is then placed in storage box, water is injected into water bath,
Ice bag cools down to water, and the test tube equipped with nucleic acid raw material is immersed in the water of low temperature, temperature sensor record water temperature, if water temperature mistake
High then cylinder will push up on storage box, and ice bag is made to be contacted with water, make water continued down, and cylinder will be under storage box if water temperature is too low
It draws, ice bag is made to completely cut off with water, prevents water freezing from freezing test tube and nucleic acid raw material.
Support frame as described above includes height adjustment device, and height adjustment device includes platform 61, perpendicular screw rod 62 and support plate 63,
Support plate is fixed on supporting rack, and support plate is located at the lower section of platform, and cylinder is located on platform, and the upper end and platform for erecting screw rod turn
Dynamic connection, perpendicular screw rod are connected with support plate thread, are erected screw rod and are passed through support plate；Height adjustment device further includes two rotation stop knots
Structure, spline structure include the dog link 64 set on the supporting plate and the pilot hole being located on platform, and platform is matched somebody with somebody with dog link slip
It closes, the glide direction of platform is parallel with the axis of dog link, in a spline structure：Dog link passes through pilot hole；Perpendicular screw rod
Lower end be equipped with for rotating vertical screw rod regulation handle 65；The sidewall horizontal of water bath extends inwardly to form baffle ring 13, test tube
It is erected on baffle ring；The material of storage box is foamed plastics；Temperature sensor is located at the lower section of rack for test tube, and temperature sensor is close
Rack for test tube.
The stroke of existing cylinder is mostly to be fixed, and the height residing for the adjustable displacements of cylinders of height adjustment device, so as to adjust
The high-low limit position of storage box is saved, after the multiple refrigeration effect of ice bag reduces, the height of cylinder can be turned down, storage box is in down
During extreme position, inlet slot can expose sliding sleeve, so as to which ice bag is taken out the ice bag frozen for freezing or more renewing again,
The specific adjusting method of cylinder height is：Rotating vertical screw rod, rises or falls platform, since the upper end of platform and perpendicular screw rod turns
Dynamic connection, platform can't significantly be rotated with the rotation of perpendicular screw rod, and arresting lever thoroughly limits the rotation of platform, is rotated with hand
Regulation handle can rotating vertical screw rod make platform that can only rise or fall, without rotating, beneficial to the stability of cylinder operation, side
Just it is quick；Baffle ring is used for holding test tubes frame, it is convenient to replace the rack for test tube of different size, and baffle ring can play mark water level
Effect, the water for facilitating control that need to inject enable test tube lower end to be immersed in the water, and keep low-temperature condition；Storage box uses foam
Plastics are made, and have both the features such as light, adiabatic, water absorption rate is low, shockproof, corrosion-resistant, are suitble to the building ring of storage box in the present invention
Border；Temperature sensor is located proximate to rack for test tube, makes the water temperature measured close to the water temperature at test tube.
The beneficial effects of the invention are as follows：The water in water bath is made to cool down by ice bag, the cryogenic effect of water-bath is better than trash ice,
And there is no the security risks that trash ice scratches experimenter；Temperature sensor can measure the temperature of water in water bath, pass through gas
Cylinder is slided up and down by storage box, contact or isolation of the control ice bag with water, to prevent water temperature over-high from causing the failure of an experiment or water temperature
It is too low to cause to freeze；It can be recycled after ice bag frost, it is at low cost, and ice bag high cooling efficiency, it is easy to use.
Claims (7)
1. a kind of use for laboratory nucleic acid extraction operation console, it is characterized in that, including
Water bath, rack for test tube, cylinder, ice bag, storage box and supporting rack, the water bath and cylinder are each provided on supporting rack, gas
Cylinder is located at the lower section of water bath, and cylinder includes cylinder body and vertically moving piston rod, and the bottom of water bath is equipped with sliding sleeve,
Storage box is slidably connected with sliding sleeve, and the glide direction of storage box is vertical, and ice bag is located in storage box, and storage box is equipped with to deposit
Take the inlet slot of ice bag；
Sealing ring and lower seal are arranged on the storage box, inlet slot is sliding between upper sealing ring and lower seal
The height of set is more than the spacing of upper sealing ring and lower seal, and test tube is erected in water bath, and rack for test tube is located at the upper of storage box
Side, rack for test tube are equipped with several test tubes for being used to store nucleic acid raw material, are equipped with to monitor the temperature sensing of water temperature in water bath
Device.
2. a kind of use for laboratory nucleic acid extraction operation console according to claim 1, it is characterized in that, support frame as described above includes height
Regulating device is spent, height adjustment device includes platform, perpendicular screw rod and support plate, support plate and is fixed on supporting rack, support plate position
In the lower section of platform, cylinder is located on platform, and the upper end and platform for erecting screw rod are rotatablely connected, and is erected screw rod and is connected with support plate thread
It connects, erects screw rod and pass through support plate.
3. a kind of use for laboratory nucleic acid extraction operation console according to claim 2, it is characterized in that, the height adjustment device
Two spline structures are further included, spline structure includes the dog link set on the supporting plate and the pilot hole being located on platform, platform
It is slidably matched with dog link, the glide direction of platform is parallel with the axis of dog link, in a spline structure：Dog link passes through
Pilot hole.
4. a kind of use for laboratory nucleic acid extraction operation console according to claim 2, it is characterized in that, the lower end of the perpendicular screw rod
Equipped with the regulation handle for rotating vertical screw rod.
5. a kind of use for laboratory nucleic acid extraction operation console according to claim 1 or 2, it is characterized in that, the water bath
Sidewall horizontal extends inwardly to form baffle ring, and test tube is erected on baffle ring.
6. a kind of use for laboratory nucleic acid extraction operation console according to claim 1 or 2, it is characterized in that, the storage box
Material is foamed plastics.
7. a kind of use for laboratory nucleic acid extraction operation console according to claim 1 or 2, it is characterized in that, the temperature sensing
Device is located at the lower section of rack for test tube, and temperature sensor is close to rack for test tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711251468.8A CN108048312B (en) | 2017-12-01 | 2017-12-01 | Nucleic acid extraction operation panel is used in laboratory |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711251468.8A CN108048312B (en) | 2017-12-01 | 2017-12-01 | Nucleic acid extraction operation panel is used in laboratory |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108048312A true CN108048312A (en) | 2018-05-18 |
| CN108048312B CN108048312B (en) | 2020-12-29 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711251468.8A Active CN108048312B (en) | 2017-12-01 | 2017-12-01 | Nucleic acid extraction operation panel is used in laboratory |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5419152A (en) * | 1993-12-13 | 1995-05-30 | In Vitro Technologies, Inc. | Apparatus for packaging temperature sensitive materials for transportation |
| US20070286775A1 (en) * | 2006-06-07 | 2007-12-13 | Yong Peter A K | Transporter |
| CN201737927U (en) * | 2009-09-25 | 2011-02-09 | 唐彬秩 | Convenient low-temperature biological operating table |
| CN203061200U (en) * | 2013-02-01 | 2013-07-17 | 山东出入境检验检疫局检验检疫技术中心 | Rapid cooling device for chemical experiment |
| CN205576143U (en) * | 2016-04-28 | 2016-09-14 | 深圳市圣必智科技开发有限公司 | Portable multi -functional kit |
| CN206139201U (en) * | 2016-10-31 | 2017-05-03 | 天津铭恒科技发展有限公司 | Shake even medical kit fast |
-
2017
- 2017-12-01 CN CN201711251468.8A patent/CN108048312B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5419152A (en) * | 1993-12-13 | 1995-05-30 | In Vitro Technologies, Inc. | Apparatus for packaging temperature sensitive materials for transportation |
| US20070286775A1 (en) * | 2006-06-07 | 2007-12-13 | Yong Peter A K | Transporter |
| CN201737927U (en) * | 2009-09-25 | 2011-02-09 | 唐彬秩 | Convenient low-temperature biological operating table |
| CN203061200U (en) * | 2013-02-01 | 2013-07-17 | 山东出入境检验检疫局检验检疫技术中心 | Rapid cooling device for chemical experiment |
| CN205576143U (en) * | 2016-04-28 | 2016-09-14 | 深圳市圣必智科技开发有限公司 | Portable multi -functional kit |
| CN206139201U (en) * | 2016-10-31 | 2017-05-03 | 天津铭恒科技发展有限公司 | Shake even medical kit fast |
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| Publication number | Publication date |
|---|---|
| CN108048312B (en) | 2020-12-29 |
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