CN108042465A

CN108042465A - Chinese medicine composition and face mask of traditional Chinese medicine

Info

Publication number: CN108042465A
Application number: CN201711311456.XA
Authority: CN
Inventors: 李文艳
Original assignee: SHANGHAI PUDONG NEW AREA PUBLIC HOSPITAL
Current assignee: SHANGHAI PUDONG NEW AREA PUBLIC HOSPITAL
Priority date: 2017-12-11
Filing date: 2017-12-11
Publication date: 2018-05-18

Abstract

The present invention provides a kind of Chinese medicine composition and face mask of traditional Chinese medicine, the Chinese medicine composition include at least：The component of following parts by weight：Raw 15 20 parts of Rhizoma Atractylodis Macrocephalae, 15 20 parts of bletilla, 12 20 parts of radix ampelopsis, 15 20 parts of PORIA ALBA, 12 18 parts of Radix Angelicae Sinensis, 12 18 parts of Rhizoma Chuanxiong, 9 15 parts of pearl powder, 9 15 parts of almond.The Chinese medicine composition can be used for preparing facial mask, have good whitening effect, non-stimulated.

Description

Chinese medicine composition and face mask of traditional Chinese medicine

Technical field

The present invention relates to a kind of Chinese medicine composition and face mask of traditional Chinese medicine, belong to tcm field.

Background technology

Chinese medicine cosmetic has the history of thousands of years in China, has related record in many ancient Chinese medicine masterpieces.Spring and autumn Period《The Classic of Mountains and Rivers》Being first has Chinese medicine cosmetic recorded books, contains several medicinal materials such as Xun's grass；And the Later Qin Dynasty Chinese period《Sheng Nong's herbal classic》Describe the herbal medicine of more than 20 kind tool beauty functions；The Ming Dynasty《Compendium of Materia Medica》168 tastes have been recorded, And the successive dynasties collect an at most most detailed ancient books and records of Chinese medicine cosmetic.

Face mask of traditional Chinese medicine is one kind of external therapy and a kind of common formulations of Chinese medicine cosmetic in Chinese herbal medicine beauty method, It is applied to face using traditional Chinese medical theory as guidance after being fused using other discrete phases such as medium-height grass the effective elements of the medicine and filmogen Portion forms a tunic or the covering not formed a film on the skin, so as to reach skin care, moisturizing, cleaning, treatment skin the effects that. Compared with other facial masks, the most significant feature of face mask of traditional Chinese medicine is that have apparent functional and specific aim, and comes from natural, work It is small with mitigation, toxic side effect.

But face mask of traditional Chinese medicine whitening effect existing on the market is bad, stronger to skin irritation at present.

The content of the invention

In view of the foregoing deficiencies of prior art, it is an object of the invention to provide a kind of Chinese medicine composition and its use On the way, for solving the problem of that the bad irritation of facial mask whitening effect is big in the prior art.

In order to achieve the above objects and other related objects, the present invention provides a kind of Chinese medicine composition, the Chinese medicine composition Include the component of following parts by weight：

Raw 15-20 parts of Rhizoma Atractylodis Macrocephalae, 15-20 parts of bletilla, 12-20 parts of radix ampelopsis, 15-20 parts of PORIA ALBA, 12-18 parts of Radix Angelicae Sinensis, Rhizoma Chuanxiong 12-18 parts, 9-15 parts of pearl powder, 9-15 parts of almond.

Further, the Chinese medicine composition further includes the component of following parts by weight：6-12 parts of motherwort, coix seed 12- 18 parts.

It is highly preferred that the Chinese medicine composition contains 20 parts of the Rhizoma Atractylodis Macrocephalae of component life, 20 parts of bletilla, radix ampelopsis 15 of following parts by weight Part, 20 parts of PORIA ALBA, 15 parts of Radix Angelicae Sinensis, 15 parts of Rhizoma Chuanxiong, 10 parts of pearl powder, 10 parts of almond, 9 parts of motherwort, 15 parts of coix seed.

Rhizoma Atractylodis Macrocephalae, this product are the dry rhizome of feverfew Rhizoma Atractylodis Macrocephalae Atractylodes macrocephala Koidz., bitter, It is sweet, it is warm-natured, enter spleen, stomach, replenish qi to invigorate the spleen, eliminate dampness and have diuretic effect.《Haigoushen》Claiming it, " interarea light is pleased, and is preserved youthful looks, and is gone.”《Newly repair Book on Chinese herbal medicine》It carries " diuresis and it being soaked with Bitters, with the face of wiping, pole effect ".

Bletilla is the dry tuber of orchid bletilla Bletilla striata (Thunb.) Reichb.f.；It is bitter, puckery, Cold nature attaches to the lung and stomach meridians, astringing to arrest bleeding, detumescence and promoting granulation.《Haigoushen》It is claimed " to control Shao- blisters on face, make us flesh cunning.”《Herbal guiding principle Mesh》It is loaded with that " bletilla is washed one's face, and is gone”。

Radix ampelopsis, this product are the dried root of vitaceae radix ampelopsis Ampelopsis japonica (Thunb.) Makino； Bitter, pungent, micro-sweet, cold nature, the thoughts of returning home, liver, the spleen channel, clearing heat and detoxicating, mass dissipating and swelling eliminating, myogenic sore.《Sheng Nong's herbal classic》Carry " main carbuncle Swollen subcutaneous ulcer sore, dissipating bind gas.”《Haigoushen》It says:" control blister sore on face.

PORIA ALBA cuts the white portion after poria cocos for medicinal material Poria cocos, and Poria cocos is On Polyporaceae Poria cocos Poria The dry sclerotia of cocos (Schw.) Wolf；It is sweet, light, mild-natured, the thoughts of returning home, stomach, lung, kidney channel, clearing damp and promoting diuresis, strengthening spleen and middle warmer, calming heart Calm the nerves.《It fills a vacancy Handbook of Prescriptions for Emergencies》Record " is controlled”。

In addition, Chinese traditional medicine angelica, the dry root for being umbelliferae angelica Angelica sinensis (Oliv.) Diels. Sweet, pungent, warm-natured, Return liver, the heart, the spleen channel enrich blood, invigorate blood circulation, and menstruction regulating and pain relieving relaxes bowel.China's the Tang Dynasty longevity name doctor's SUN Si miao writes 's《Qianjin Yi Fang》In anti-ageing, ecchymose removing, beauty, strong skin " married woman face medicine " and Wang Tao written by《Medical secrets of official》Middle Radix Angelicae Sinensis uses frequency Rate is high.Present age research also indicates that it has a better effect for freckle, blackspot, senile plaque expelling.

Rhizoma Chuanxiong, this product are samphire Rhizoma Chuanxiong Ligusticum chuanxiong Hort. (or Ligusticum Wallichii Franch.) dry rhizome.It is pungent, warm-natured, Return liver, courage, pericardium channel, blood-activating and qi-promoting, wind-expelling pain-stopping.《Between outer land is secret It will》Middle whitening spot-removing, dissipating bind have effects that pungent perfume is fluent, drug of dispelling wind and removing obstruction in the meridians except scar external application side often selects, Rhizoma Chuanxiong；It is beneficial female Grass, bitter, pungent, cold nature, Return liver, pericardium, bladder warp, promoting blood circulation for regulating menstruation, inducing diuresis to remove edema are clearing heat and detoxicating.The Tang Dynasty pharmacy man Chen Cangqi 《Bencao Shiyi》Described in " enter face medicine, make us gloss, control acne." modern medicine beauty research show that motherwort can promote Skin metabolism, in anti-ageing anti-cancer, skin maintenance, promote longevity on have remarkable efficacy；

Pearl, this product is Pteriidae animal Pinctada martensii Pteria martensii (Dunker), Unionidae animal three The bivalves such as angle sail freshwater mussel Hyriopsis cumingii (Lea) or cristaria plicata Cristaria plicata (Leach) The pearl of stimulated formation；Sweet, salty, cold in nature, converge to heart and liver channels, arresting convulsion of calming the nerves, improving eyesight disappears screen, solves taking poison and growing muscle due, moistening skin and eliminating spot.It is bright In generation, medical family's Li Shizhen (1518-1593 A.D.) existed《Compendium of Materia Medica》Middle load：" face is applied, makes us moist good color." now pearl face method be that the world is each State is used.

Coix seed is grass Job's tears Coix lacryma-jobi L.var.ma-yuen (Roman.) Stapf's Dry mature kernal；It is sweet, light, cool in nature, returns spleen, stomach, lung channel.Clearing damp and promoting diuresis, invigorating the spleen to arrest diarrhea, eliminating impediment, apocenosis, detoxicating and resolving a mass.Begin It is loaded in《Sheng Nong's herbal classic》.Modern study confirms that coix seed is rich in protein, and vitamin B1, B2 can keep skin gloss thin It is greasy, there is the effect of fine eliminating acne, freckle, senile plaque expelling, release-prevention, pachylosis etc.；

Almond, Amygdalus Communis Vas are bitter for the dry seed of the bitters such as rosaceous plant apricot or ansu apricot, Slightly warm in nature, return lung, large intestine channel.Sending down abnormally ascending is relieving cough and asthma, relaxes bowel.Contain abundant VitAVitE, sub- oil in almond Acid etc., to face acnes, pachylosis, pigmentation etc. have preferable preventive and therapeutic effect, make skin cleaning it is beautiful, rich in gloss And elasticity.

Motherwort, this product are the fresh of labiate motherwort Leonurus japonicus Houtt. or dry ground Upper part.

Preferably, the Chinese medicine composition is powdered or graininess.It is obtained after being crushed using above-mentioned Chinese medicine composition.

Another aspect provides the purposes that above-mentioned Chinese medicine composition is used to prepare skin care item or cosmetics.

Another aspect provides the purposes that above-mentioned Chinese medicine composition is used to prepare facial mask.

Another aspect provides a kind of face mask of traditional Chinese medicine, the face mask of traditional Chinese medicine include above-mentioned Chinese medicine composition and The acceptable auxiliary material of human body.

Further, the auxiliary material is honey.

Another aspect provides Chinese medical extract, the Chinese medical extract uses Chinese traditional medicine composition as described above Object is made for raw material effective component extracting.

In the present invention, the formula of each component is the most key in Chinese medicine composition, after Chinese medicine preparation is known, the art Technical staff various conventional traditional Chinese medicine extyaction methods can be used to extract its active ingredient, such as conventional decoction, Water extraction and alcohol precipitation method, ethanol extract from water precipitation, salting out method etc. can obtain the main medicine in foregoing Chinese medicine preparation due to said extracted method Ingredient is imitated, therefore can have certain inhibitory action to tyrosinase.

Wherein, water extraction and alcohol precipitation method is：First using water as solvent extraction medicinal material active ingredient, then the ethanol precipitation with various concentration The method for removing impurity in extracting solution.When generally reaching 50%~60% (w/v i.e. g/100ml) containing amount of alcohol, it can go Except impurity such as starch, when alcohol content is up to more than 75%, in addition to a small number of invalid components such as tannin, water colo(u)r, remaining major part The precipitable removal of impurity.

Ethanol extract from water precipitation is：Medicinal ingredient is first extracted with the ethyl alcohol of suitable concentration, then impurity in extracting solution is removed with water Method.

It can be carried again through conventional step concentration, purifying or the dry Chinese medicine that obtains after the Chinese medicine composition effective component extracting Take object.The method wherein concentrated includes but not limited to：Atmospheric evaporation, reduction vaporization, thin film evaporation, multiple-effect evaporation, the side of purifying Method includes but not limited to：Saltout purifying, macroporous resin adsorption purifying, ethanol precipitation purifying, ion-exchange resin purification, flocculation is heavy Form sediment purifying, membrane separating and purifying, polycaprolactam purifying, silica gel absorption chromatographic purifying etc., and dry method includes but not limited to：It dries Dry method, drum-type seasoning, belt drying method, hygroscopic desiccation method, fluid-bed drying, spray drying process, hypobaric drying method, freezing are dry Dry method, infrared drying, micro-wave drying method etc..

According to the characteristic of different crude drug active ingredients in the Chinese medicine composition, different extraction processes can be used.

Another aspect of the present invention additionally provides a kind of preparation method of Chinese medical extract, the method includes the steps：It presses Proportioning takes each taste traditional Chinese medicinal components, and water immersion is increased after mixing, you can.

Another aspect provides purposes of the above-mentioned Chinese medical extract in skin care item or cosmetics are prepared.

Another aspect provides the purposes that above-mentioned Chinese medical extract is used to prepare facial mask.

It, can containing above-mentioned Chinese medical extract and human body on the facial mask another aspect provides a kind of facial mask With the auxiliary material of receiving.

Further, the auxiliary material used in conventional dose may be employed in the auxiliary material.

As described above, Chinese medicine composition of the present invention and application thereof, has the advantages that：

Tyrosinase activity can effectively be inhibited, it is non-stimulated to skin, it can be used as skin-whitening product.Especially It is when the concentration of the Chinese medicine composition is 468.9 μ g/mL, inhibition efficiency reaches maximum.

Description of the drawings

Fig. 1 is shown as tyrosine time of enzymatic reacting curve synoptic diagram in embodiment 1.

Fig. 2 is shown as 1 various concentration sample of embodiment to inhibitory activity against tyrosinase schematic diagram.

Fig. 3 a are shown as blank control group HE coloration result figures in embodiment 3.

Fig. 3 b are shown as model group HE coloration result figures in embodiment 3.

Fig. 3 c are shown as honey matrix group HE coloration result figures in embodiment 3.

Fig. 3 d are shown as positive drug control group HE coloration result figures in embodiment 3.

Fig. 3 e are shown as returning white solidifying flesh side's high dose group HE coloration result figures in embodiment 3.

Fig. 3 f are shown as returning white solidifying flesh side's low dose group HE coloration result figures in embodiment 3.

Fig. 4 a are shown as blank control group HE coloration result figures in embodiment 4.

Fig. 4 b are shown as model group HE coloration result figures in embodiment 4.

Fig. 4 c are shown as honey matrix group HE coloration result figures in embodiment 4.

Fig. 4 d are shown as 4 positive drug control group HE coloration result figures of embodiment.

Fig. 4 e are shown as embodiment 4 and return white solidifying flesh side's high dose group HE coloration result figures.

Fig. 4 f are shown as returning white solidifying flesh side's low dose group HE coloration result figures in embodiment 4.

Fig. 5 a are shown as the influence for returning white solidifying flesh side to SOD in pigmentation guinea pig model tissue.

Fig. 5 b are shown as the influence for returning white solidifying flesh side to MDA in pigmentation guinea pig model tissue.

Fig. 6 a are shown as No. 1 pathological section HE coloration result figure of facial mask group in embodiment 6.

Fig. 6 b are shown as No. 2 pathological section HE coloration result figures of facial mask group in embodiment 6.

Fig. 6 c are shown as No. 3 pathological section HE coloration result figures of facial mask group in embodiment 6.

Fig. 6 d are shown as No. 1 pathological section HE coloration result figure of 6 mesostroma group of embodiment.

Fig. 6 e are shown as No. 2 pathological section HE coloration result figures of 6 mesostroma group of embodiment.

Fig. 6 f are shown as No. 3 pathological section HE coloration result figures of 6 mesostroma group of embodiment.

Specific embodiment

Illustrate embodiments of the present invention below by way of specific specific example, those skilled in the art can be by this specification Disclosed content understands other advantages and effect of the present invention easily.The present invention can also pass through in addition different specific realities The mode of applying is embodied or practiced, the various details in this specification can also be based on different viewpoints with application, without departing from Various modifications or alterations are carried out under the spirit of the present invention.

Bulk pharmaceutical chemicals (or medicinal material) of the present invention can be bought from common pharmacy or Chinese medicine sales company It arrives, specification meets national medical standards or meets the pertinent regulations such as Chinese Pharmacopoeia.Used medicinal material unless otherwise indicated, is Tcm and herbal slice, the tcm and herbal slice can also be through being process after obtaining.

Return the white Chinese medicine composition returned in the white solidifying flesh side i.e. present invention below

1 pharmacy in vitro of embodiment is studied

Research background：Between the Epidermal basal layer cells of people, melanocyte is dispersed with, the tyrosinase that it contains can incite somebody to action Oxidizing tyrosine can finally generate melanin, so tyrosinase is into polysaccharide, centre using a series of metabolic process The key enzyme of B16 cell.Melanin generation is more, and skin is pitch-darker；Conversely, then skin is more pale.Therefore, with junket Propylhomoserin inhibition of enzyme activity rate is index, measures absorbance, and to returning white solidifying flesh side the effect of is evaluated.

Experimental method：L-tyrosine solution (0.25mmol/L), the 20 μ L phosphate-buffereds of 220 μ L are added in 96 orifice plates Solution (pH=6.8), mixing add in 10 μ L-Tyrs enzyme solutions (268.7U/mL) after Warm incubates 10min in 30 DEG C of water-baths, So that final volume is 250 μ L, its absorbance is measured at 475nm wavelength, continuous record to 160min.

Following four groups of reaction solutions are added on 96 orifice plates, return white solidifying flesh side (raw Rhizoma Atractylodis Macrocephalae 20g, bletilla 20g, radix ampelopsis 15g, white Poria cocos 20g, Radix Angelicae Sinensis 15g, Rhizoma Chuanxiong 15g, pearl powder 10g, almond 10g, motherwort 9g, coix seed 15g, preparing becomes impalpable powder) Final concentration be respectively 50 μ g/mL, 100 μ g/mL, 200 μ g/mL, 500 μ g/mL, 750 μ g/mL, 1500 μ g/mL, 2500 μ g/ ML, 30 DEG C of Warm incubate 30min, measure absorbance A respectively at 475nm, and inhibiting rate is sought by formula.

1 tyrosinase inhibition test of table

Inhibiting rate=[1- (A3-A4)/(A1-A2)] × 100

Experimental result：As shown in Figure 1, absorbance growth at any time and increase, become in 15-45min times internal absorbance It is fastest to change linear reaction of propagation, after 45min, absorbance growth tends towards stability, and reaction speed slows down.Therefore, experiment choosing It negates absorbance change when answering 30min and measures enzymatic reaction speed to calculate.

As shown in table 2 and Fig. 2, return white solidifying flesh side to the apparent inhibitory action of tyrosinase activity, with returning white solidifying flesh side Concentration increases, and inhibiting rate also increases therewith.When sample concentration is more than 500 μ g/mL, the increasing degree of inhibiting rate is dense with sample The variation of degree mitigates very much.The half-inhibition concentration IC50 that Gui Bai coagulates flesh side is 468.9 μ g/mL.

2 various concentration sample of table is to inhibitory activity against tyrosinase

Experiment conclusion：Between the Epidermal basal layer cells of people, melanocyte is dispersed with, the tyrosinase that it contains can incite somebody to action Oxidizing tyrosine can finally generate melanin, so tyrosinase is into polysaccharide, centre using a series of metabolic process The key enzyme of B16 cell.Melanin generation is more, and skin is pitch-darker；Conversely, then skin is more pale.According to returning in vitro The experimental result that white solidifying flesh side influences tyrosinase activity is shown, white solidifying flesh side is returned to have apparent inhibition to make to tyrosinase With IC50 is 468.9 μ g/mL.

Embodiment 2

Research purpose：Research of the white solidifying flesh side to NB-UVB induction cavy pigmentation model effects is returned in observation.

Experimental method：Cavy is randomly divided into blank group, model group, matrix control group, positive controls, returns white solidifying flesh side Low dose group, white solidifying flesh side's high dose group of returning, every group 8.Cavy is sloughed into back hair, depilation area is about 5 × 6cm², often Week depilation 1 time.In addition to blank group, ultraviolet B radiation of other each groups using wavelength as 311nm irradiates 1 time daily, irradiates every time Time is 60min, and the distance of animal and light source is 20cm, Continuous irradiation 4 weeks.After modeling success, applied respectively at cavy skin lesion Corresponding drug is smeared, l times daily, using hydroquinone ointment as positive control drug, 0.3g/ is only uniformly applied to depilation position, and honey is 4g is returned white solidifying flesh side to add in 10mL honey and stirred evenly as high dose, white solidifying flesh side returned to add in 1g by Matrix controls It is stirred evenly in 10mL honey as low dosage.After successive administration 4 weeks, the variation feelings of record each group cavy depilation area skin Condition, and score by the standards of grading of table 3.As a result such as table 4.Animal is put to death after experiment, takes its position skin that loses hair or feathers respectively Skin sample is divided into two parts by skin tissue, and portion is used for skin histology for HE dyeing and Fontana-Masson dyeing, portion The measure of middle SOD, MDA.

3 dermoreaction standards of grading of table

Reaction	It scores
		Pigmentation
Normal skin tone	0
		Slight pigment occur increases, in pale red	1
Apparent pigment occur increases, in filbert	2
		Color is in kermesinus or dark brown	3
Black	4

Table 4 returns white solidifying flesh side to the pigmented influence of guinea pig skin

Note：Compared with blank control group, ###p<0.01；Compared with matrix control group, * p<0.05, * * p<0.01, * * * p <0.001

Experimental result：As shown in table 4, there is apparent pigmentation, honey matrix group globefish after UVB irradiates in guinea pig back The pigmentation area skin of mouse does not have significant change.After returning white solidifying flesh side and quinhydrones processing, there is corresponding color in cavy Plain calm mitigation.

Embodiment 3 carries out histopathologic examination to the experimental result in embodiment 2

As a result as Fig. 3 a~3f, HE coloration results show that blank group horny layer of epidermis cell arrangement is neat, and basal layer is visible The melanin granule of only a few, interstitial collagen fiber is without apparent hyperplasia, no lymphocytic infiltration；Compared with blank group, model group with Matrix control group epidermal thickness substantially thickens, and with dyskeratosis, the visible substantial amounts of melanin granule of basal layer, interstitial collagen fiber increases It is raw, it is seen that lymphocytic infiltration, and two groups of no significant differences；Gui Bai coagulates flesh side and slightly increases with positive controls epidermal thickness Thickness, the visible a small amount of melanin granule of basal layer return white solidifying flesh side's high dose group closest to blank group normal guinea pig epidermis, skin corium Without apparent interstitial collagen fibroplasia, lymphocytic infiltration is had no.

Embodiment 4 is to the result Fontana-Masson dyeing observation the black pigment of skin synthesis situations in embodiment 2

As a result such as Fig. 4 a~4f, compared with matrix control group, it is apparent that positive drug and Gui Bai coagulate flesh side's group melanin granule quantity It reduces.Accidental melanin granule in blank control group epidermis；Melanin granule is distributed in model group and matrix control group basal layer of epidermis Continuously, it is seen that substantial amounts of melanin granule；Gui Bai coagulates flesh side and melanin granule distribution in positive controls basal layer of epidermis is discontinuous, It can be seen that a small amount of melanin granule.

Embodiment 5 returns white solidifying flesh side to superoxide dismutase (SOD) in pigmented guinea pigs skin and malonaldehyde (MDA) The influence of content

As shown in table 5 and Fig. 5 a, 5b, guinea pig skin is after ultraviolet light irradiates, compared with Normal group, model group, base Matter control group guinea-pig studies area skin SOD value pole significantly reduces, the extremely significantly rise, and honey matrix control group and model of MDA values The no significant difference of group.Quinhydrones positive controls guinea-pig studies area skin SOD value is more than matrix control group, and with conspicuousness Difference；MDA is less than matrix control group, and with significant difference.Gui Bai coagulates high dose group guinea-pig studies area of flesh side skin SOD Value is more than blank control group, and with highly significant difference (P<0.01)；MDA values are less than blank control group, and with extremely significantly Xing Cha circle (P<0.001).Gui Bai coagulates low dose group guinea-pig studies area of flesh side skin SOD value more than blank control group, and with aobvious Zhu Xingcha circle (P<0.05)；MDA values are less than blank control group, and with significant difference (P<0.05).

Table 5 returns influence of the white solidifying flesh side to SOD, MDA content in pigmentation guinea pig model tissue

Experiment conclusion：Gui Bai, which coagulates flesh side, can raise cavy local skin superoxide dismutase activity, inhibit lipid mistake Oxidation reaction inhibits melanin granule synthesis so as to mitigate pigmentation model guinea pigs pigmentation, reaches freckle removing and whitening effect.

6 rabbit skin irritation test --- safety experiment of embodiment

Research purpose：It determines to return white solidifying flesh side's security

Experimental method：Extracting waste rabbit 6, using bare substrate as negative control group, using androgynous left and right self-contrast Method.Experiment the previous day removes family's rabbit back backbone diamond wool, area about 5cm x 5cm, as experiment and look-out station.Every time Experiment takes 3g to return white solidifying flesh side, and addition 7.5mL honey, which is mixed well, is made facial mask use.Left side, which is uniformly smeared, daily returns white solidifying flesh aspect The bare substrate of equivalent is smeared on film, right side, is covered medicine-feeding part with one layer of glass and gauze, is then consolidated with semiclosed property binder It is fixed, after continuing 4h, remove covering and clean medicine-feeding part with warm water, once a day, continuous 7 days.After removal drug every time Observe and record before 1h and again coating erythema and oedema, smear position whether have pigmentation, blutpunkte, pachylosis or Epidermatic atrophy situation and its time of origin and regression time, and score erythema and oedema.In last except removal tested material With visually observed when l, 24,48 and 72 are small after reference material, 72 it is small when after skin is taken to fix, dye carry out pathological observation.

6 skin irritation of table reacts standards of grading

Reaction	It scores
		Erythema and eschar are formed：
Without erythema	0
		Pole slight erythema (visible reluctantly)	1
Erythema is clear	2
		Moderate erythema	3
Severe erythema (aubergine) to eschar is formed	4
		Oedema：
Without oedema	0
		Extremely slight oedema (visible reluctantly)	1
Oedema is clear (being swollen with, without departing from Qu Yu Bian Vela)	2
		Intermediate edema (is swollen with about 1mm)	3
Severe edema (is swollen with more than 1mm, and beyond coating area)	4
		Highest is stimulated to score	8

7 skin irritation intensity evaluation standard of table

Mean scores	Skin irritation degree evaluation
		0~0.49	It is nonirritant
0.50~2.99	Slight stimulation
		3.00~5.99	Moderate irritation
6.00~8.00	Strong and stimulating

Table 8 returns white solidifying flesh side to rabbit skin irritation test visual results

React mean scores=(erythema forms total score+oedema and forms total score)/number of animals

Experimental result：

Visual results：As shown in table 8, last smears 1h after removal drug, for 24 hours, 48h and 72h visually observe it is tested Medicine group and bare substrate group are showed no family's rabbit skin any abnormal response, and local skin has no oedema, pigmentation, bleeding Situations such as point, pachylosis or epidermatic atrophy, show by reagent return both white solidifying flesh sides to normal skin and damaged skin not Generate macroscopic irritation.

Embodiment 7 carries out pathological examination to the experimental result in embodiment 6

Histopathologic examination's result such as Fig. 6 a-f：Facial mask group and matrix group cutaneous squamous epithelium show be it is thin, not There is hyperplasia, the epithelial cell of layer 2-3, surface is containing a small amount of cutin.A small amount of chronic inflammatory cell leaching is seen in skin corium and subcutaneous tissue The appendicles such as profit, skin follicle, sebaceous glands and eccrine gland are shown no obvious abnormalities.Skin texture is normal.

Experiment conclusion：Gui Bai coagulates flesh side's continuous and local and smears 7 days, and obvious irritation is had no to family's rabbit skin.

Above embodiment is in order to illustrate embodiment disclosed by the invention, can not be interpreted as the limit to the present invention System.In addition, in various modifications listed herein and invention method, composition variation, do not departing from the scope of the present invention Be obvious for those skilled in the art on the premise of spirit.Although a variety of specific of the present invention is combined Preferred embodiment has carried out specific description to the present invention, it is to be understood that, the present invention should not be limited only to these specific embodiments. In fact, various, obviously modification should all include to obtain invention for those skilled in the art as described above Within the scope of the invention.

Claims

1. a kind of Chinese medicine composition, which is characterized in that the Chinese medicine composition includes at least the component of following parts by weight：Raw Rhizoma Atractylodis Macrocephalae 15-20 parts, 15-20 parts of bletilla, 12-20 parts of radix ampelopsis, 15-20 parts of PORIA ALBA, 12-18 parts of Radix Angelicae Sinensis, 12-18 parts of Rhizoma Chuanxiong, pearl powder 9-15 parts, 9-15 parts of almond.

2. Chinese medicine composition according to claim 1, it is characterised in that：The Chinese medicine composition further includes following parts by weight Component：6-12 parts of motherwort, 12-18 parts of coix seed.

3. according to 1 or 2 any one of them Chinese medicine composition of claim, which is characterized in that the Chinese medicine composition is powder Shape or graininess.

4. as claims 1 to 3 any one of them Chinese medicine composition is used to prepare the purposes of skin care item or cosmetics.

5. as claims 1 to 3 any one of them Chinese medicine composition is used to prepare the purposes of facial mask.

6. a kind of face mask of traditional Chinese medicine, the face mask of traditional Chinese medicine includes Chinese medicine composition and human body as described in claims 1 to 3 Arbitrary Term Acceptable auxiliary material.

7. a kind of Chinese medical extract, which is characterized in that the Chinese medical extract uses such as claims 1 to 3 any one of them Chinese medicine composition is made for raw material effective component extracting.

8. purposes of the Chinese medical extract as claimed in claim 7 in skin care item or cosmetics are prepared.

9. purposes of the Chinese medical extract as claimed in claim 7 in facial mask is prepared.

10. a kind of facial mask, which is characterized in that the facial mask contains Chinese medical extract and human body as claimed in claim 7 can The auxiliary material of receiving.