CN108040882A - A kind of abductive approach of garlic test tube seedling - Google Patents
A kind of abductive approach of garlic test tube seedling Download PDFInfo
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- CN108040882A CN108040882A CN201810016835.4A CN201810016835A CN108040882A CN 108040882 A CN108040882 A CN 108040882A CN 201810016835 A CN201810016835 A CN 201810016835A CN 108040882 A CN108040882 A CN 108040882A
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- garlic
- aerosol index
- test tube
- tube seedling
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Abstract
The present invention provides a kind of abductive approach of garlic test tube seedling, prepared including culture medium, aerosol index processing, inoculation, aerosol index is used into GA first3Solution impregnates, break the dormancy of garlic aerosol index, improve inductivity, shorten development time, then aerosol index after breaking dormancy is inoculated into in the culture medium prepared culture under given conditions obtain test tube seedling, plant growth regulator NAA and 6 BA containing certain concentration in the culture medium prepared, specified plant growth regulator are added with the inductivity for being beneficial to improve test tube seedling.The present invention directly induces test tube seedling by the use of garlic aerosol index as explant, while making garlic realization natural detoxification, avoid the dedifferentiation of callus approach, break up again, breeding coefficient is low and is also easy to produce the shortcoming of hereditary variation, lay the first stone for the detoxifying fast breeding of garlic；Also, the time that test tube seedling is cultivated using method provided by the invention is greatly shortened, and improves emergence rate.
Description
Technical field
The present invention relates to agro-biological engineering technical fields more particularly to a kind of utilization garlic aerosol index to cultivate garlic children
The method of seedling.
Background technology
Garlic（Allium sativumL.）For asexually propagated crop, bred usually using bulb, due to long-time service squama
Stem breeding, virus are accumulated by generation, along with the influence of unsuitable environmental condition, bad cultivation step, cause garlic " degeneration " phenomenon
Seriously, extreme loss is caused to production.To avoid the generation of the above situation, people use garlic aerosol index（Its garlic）Breeding,
Garlic uses aerosol index（Its garlic）During breeding, aerosol index picks up from garlic stems（Flower a kind of sedge）The petal on top（Growing point）, petal one
As without virus, therefore, can natural detoxification using aerosol index.But aerosol index has the phenomenon that dormancy, extends and utilizes gas
Raw bulb obtains the cycle of seedling, adds time cost, and therefore, it is very to establish a kind of new efficient garlic rapid propagation system
It is necessary.
The content of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of abductive approach of garlic test tube seedling, to solve background technology
The problem of middle virus is accumulated by generation.
The present invention is achieved through the following technical solutions, provides a kind of abductive approach of garlic test tube seedling, the method
Include the following steps：
S01：The preparation of culture medium：Using MS culture mediums as minimal medium, add in sucrose, agar, plant growth regulator NAA and
6-BA boils and dissolves agar, and pH value is adjusted to 5.8~6.0, is dispensed into tissue culture bottle, lid lid sealing, autoclave sterilization standby
With.
S02：Aerosol index processing：By the aerosol index got ready in GA3Flowing water rinses after being impregnated in solution, is then transferred to
Alcohol disinfecting, aseptic water washing, hypochlorite disinfectant are carried out in superclean bench, finally with spare after aseptic water washing.
In the present invention, first by the aerosol index got ready in GA3It is impregnated in solution, is conducive to break aerosol index dormancy,
Improve inductivity.
S03：Inoculation：On superclean bench, with the blotting paper after sterilizing by the surface moisture for the aerosol index handled well
It blots, aerosol index is inoculated into the culture medium prepared in the step S01 with the tweezers after sterilizing, be transferred to the training of tissue culture room
It supports, illumination condition is 2600 lx, 16 hd-1, room temperature is 23~27 DEG C.
Preferably, in the step S01, using MS culture mediums as minimal medium, sucrose 30 is added in 1 L culture mediums
G, 7 g of agar, and add the 6-BA of the NAA and 0.3mg/L of plant growth regulator 0.05mg/L, it boils and dissolves agar, will cultivate
Base is settled to 1000 ml, and pH value is adjusted to 5.8~6.0, is dispensed into tissue culture bottle, every bottle of 30 ml culture mediums, lid lid sealing, and 121
It is spare after DEG C 20 min of autoclave sterilization.
Each content of material is highly important to the induction of garlic test tube seedling in culture medium, and the present invention is real by repeatedly optimization
It tests and learns, when being cultivated using the culture medium of above-mentioned content, the inductivity of garlic test tube seedling is highest, particularly plant life
The concentration of long conditioning agent, the inductivity of test tube seedling can be improved by adding the hormone of above-mentioned concentration.
Preferably, in the step S01, the MS culture medium prescriptions are：Contain KNO in every 1 L minimal mediums3
1900mg, NH4NO31650mg, MgSO4·7H2O 370mg, KH2PO4170mg, CaCl2·2H2O 440mg, MnSO4·
4H2O 22.3mg, ZnSO4·7H2O 8.6mg, H3BO36.2mg, KI 0.83mg, Na2MO3·2H2O 0.25mg, CuSO4·
5H2O 0.025mg, CoCl2·6H2O 0.025mg, Na2- EDTA 37.3mg, FeSO4·7H2O 27.8mg, glycine
2.0mg, thiamine hydrochloride 0.1mg, puridoxine hydrochloride 0.5mg, niacin 0.5mg, inositol 100mg.
Preferably, in the step S02, by the aerosol index got ready 100~150mg/L GA3It is impregnated in solution
12~24 it is small when, with flowing water rinse 5~10 min after be transferred in superclean bench, 30~60 s of alcohol disinfecting, sterile water punching
2~3 times, 15~20min of hypochlorite disinfectant are washed, finally with aseptic water washing 3~5 times.
Preferably, in the step S02, the aerosol index got ready is：Removal has induced the big of aerosol index
The coat of garlic flower bud and false stem, the single aerosol index removed., it is necessary to induce garlic first before this operation is carried out
Aerosol index.
Technical solution provided in an embodiment of the present invention can include following advantageous effect：
The present invention provides a kind of abductive approach of garlic test tube seedling, including steps such as culture medium preparation, aerosol index processing, inoculations
Suddenly, aerosol index is used into GA first3Solution impregnates, and breaks the dormancy of garlic aerosol index, improves inductivity, when shortening development
Between, then the aerosol index after breaking dormancy is inoculated into in the culture medium prepared culture under given conditions obtain test tube children
Seedling, plant growth regulator NAA and 6-BA containing certain concentration in the culture medium prepared, specified plant growth regulator
Added with beneficial to the inductivity for improving test tube seedling.The present invention directly induces test tube seedling by the use of garlic aerosol index as explant,
Make garlic realize natural detoxification while, avoid the dedifferentiation of callus approach, break up again, breeding coefficient is low and is also easy to produce something lost
The different shortcoming of the progress of disease lays the first stone for the detoxifying fast breeding of garlic；Also, test tube seedling is cultivated using method provided by the invention
Time greatly shortens, and improves emergence rate.
Specific embodiment
In order to which those skilled in the art is made to more fully understand the technical solution in the present invention, below to the embodiment of the present invention
In technical solution be clearly and completely described, it is clear that described embodiment be only part of the embodiment of the present invention,
Instead of all the embodiments.Based on the embodiments of the present invention, those skilled in the art are not before creative work is made
All other embodiments obtained are put, should all be belonged to the scope of protection of the present invention.
Embodiment 1
The present embodiment provides a kind of abductive approach of garlic test tube seedling, the method carries out in accordance with the following steps：
S01：The preparation of culture medium：Using MS culture mediums as minimal medium, 30 g of sucrose, 7 g of agar are added in 1 L culture mediums,
And add the 6-BA of the NAA and 0.3mg/L of plant growth regulator 0.05mg/L, it boils and dissolves agar, culture medium is settled to
1000 ml, pH value are adjusted to 5.8, are dispensed into tissue culture bottle, every bottle of 30 ml culture mediums, lid lid sealing, 121 DEG C of autoclave sterilizations
20 min, it is spare.
S02：Aerosol index processing：The coat and false stem that the garlic petal of aerosol index will have been induced remove, and remove
Single aerosol index, it is spare；Then by the aerosol index got ready in GA3When immersion 12 is small in solution, after rinsing 5 min with flowing water
It is transferred in superclean bench, 30 s of alcohol disinfecting, aseptic water washing 3 times, 15 min of hypochlorite disinfectant finally uses sterile water
It rinses 3 times.
S03：Inoculation：On superclean bench, with the blotting paper after sterilizing by the surface moisture of the aerosol index sterilized
It blots, aerosol index is inoculated into the culture medium prepared in the step S01 with the tweezers after sterilizing, be transferred to the training of tissue culture room
It supports, illumination condition is 2600 lx, 16 h d-1, room temperature is 23 DEG C.Culture counts Allium Sativum L inductivity after 30 days, such as table 1
It is shown.
Embodiment 2
The present embodiment provides a kind of abductive approach of garlic test tube seedling, the method carries out in accordance with the following steps：
S01：The preparation of culture medium：Using MS culture mediums as minimal medium, 30 g of sucrose, 7 g of agar are added in 1 L culture mediums,
And add the 6-BA of the NAA and 0.3mg/L of plant growth regulator 0.05mg/L, it boils and dissolves agar, culture medium is settled to
1000 ml, pH value are adjusted to 6.0, are dispensed into tissue culture bottle, every bottle of 30 ml culture mediums, lid lid sealing, 121 DEG C of autoclave sterilizations
20 min, it is spare.
S02：Aerosol index processing：The coat and false stem that the garlic petal of aerosol index will have been induced remove, and remove
Single aerosol index, it is spare；Then by the aerosol index got ready in GA3When immersion 24 is small in solution, 10 min are rinsed with flowing water
After be transferred in superclean bench, 60 s of alcohol disinfecting, aseptic water washing 3 times, 20 min of hypochlorite disinfectant, finally with sterile
Water rinses 5 times.
S03：Inoculation：On superclean bench, with the blotting paper after sterilizing by the surface moisture of the aerosol index sterilized
It blots, aerosol index is inoculated into the culture medium prepared in the step S01 with the tweezers after sterilizing, be transferred to the training of tissue culture room
It supports, illumination condition is 2600 lx, 16 h d-1, room temperature is 27 DEG C.Culture counts Allium Sativum L inductivity after 30 days, such as table 1
It is shown.
Embodiment 3
The present embodiment provides a kind of abductive approach of garlic test tube seedling, the method carries out in accordance with the following steps：
S01：The preparation of culture medium：Using MS culture mediums as minimal medium, 30 g of sucrose, 7 g of agar are added in 1 L culture mediums,
And add the 6-BA of the NAA and 0.3mg/L of plant growth regulator 0.05mg/L, it boils and dissolves agar, culture medium is settled to
1000 ml, pH value are adjusted to 5.8, are dispensed into tissue culture bottle, every bottle of 30 ml culture mediums, lid lid sealing, 121 DEG C of autoclave sterilizations
20 min, it is spare.
S02：Aerosol index processing：The coat and false stem that the garlic petal of aerosol index will have been induced remove, and remove
Single aerosol index, it is spare；Then by the aerosol index got ready in GA3When immersion 20 is small in solution, after rinsing 8 min with flowing water
It is transferred in superclean bench, 30 s of alcohol disinfecting, aseptic water washing 3 times, 15 min of hypochlorite disinfectant finally uses sterile water
It rinses 4 times.
S03：Inoculation：On superclean bench, with the blotting paper after sterilizing by the surface moisture of the aerosol index sterilized
It blots, aerosol index is inoculated into the culture medium prepared in the step S01 with the tweezers after sterilizing, be transferred to the training of tissue culture room
It supports, illumination condition is 2600 lx, 16 h d-1, room temperature is 25 DEG C.Culture counts Allium Sativum L inductivity after 30 days, such as table 1
It is shown.
Embodiment 4
The present embodiment provides a kind of abductive approach of garlic test tube seedling, the method carries out in accordance with the following steps：
S01：The preparation of culture medium：Using MS culture mediums as minimal medium, 30 g of sucrose, 7 g of agar are added in 1 L culture mediums,
And add the 6-BA of the NAA and 0.3mg/L of plant growth regulator 0.05mg/L, it boils and dissolves agar, culture medium is settled to
1000 ml, pH value are adjusted to 5.8, are dispensed into tissue culture bottle, every bottle of 30 ml culture mediums, lid lid sealing, 121 DEG C of autoclave sterilizations
20 min, it is spare.
S02：Aerosol index processing：The coat and false stem that the garlic petal of aerosol index will have been induced remove, and remove
Single aerosol index, it is spare；Then by the aerosol index got ready in GA3When immersion 16 is small in solution, after rinsing 7 min with flowing water
It is transferred in superclean bench, 30 s of alcohol disinfecting, aseptic water washing 2 times, 15 min of hypochlorite disinfectant finally uses sterile water
It rinses 5 times.
S03：Inoculation：On superclean bench, with the blotting paper after sterilizing by the surface moisture of the aerosol index sterilized
It blots, aerosol index is inoculated into the culture medium prepared in the step S01 with the tweezers after sterilizing, be transferred to the training of tissue culture room
It supports, illumination condition is 2600 lx, 16 h d-1, room temperature is 25 DEG C.Culture counts Allium Sativum L inductivity after 30 days, such as table 1
It is shown.
Comparative example 1
In the present embodiment, the disease-free garlic clove of health is selected, peels off outer layer protection leaf, the garlic heel of removal base portion brown, cut 0.3~
0.4cm garlic clove base portion stem disks, 84 disinfection night disinfection 30s, aseptic water washing 3 ~ 5 times, 0.1% mercuric chloride disinfection 15min, sterile water
It rinses 3~5 times.It cuts with a knife and is segmented into 0.5cm2Fritter, be inoculated into addition 2, in the calli induction media of 4-D, induce
It is transferred to after callus in bud differential medium.Pipe bud induction rate is counted after 30 days, as shown in table 1.
Comparative example 2
In the present embodiment, the fresh garlic stems petal (band crust) for not yet forming aerosol index is taken to cut base portion garlic shoot, alcohol disappears
Malicious 1min, 0.1% mercuric chloride disinfection 20min, aseptic water washing 5 times are inoculated into after peelling off luxuriant skin on adventitious bud induction culture base,
It is transferred to after growing up to adventitious bud on test tube seedling inducing culture.Allium Sativum L inductivity is counted after 30 days, as shown in table 1.
Comparative example 3
In the present embodiment, the coat of the garlic petal of aerosol index and false stem are removed, single aerosol index is removed, by stripping
Aerosol index is cultivated in nursery, is grown naturally.Allium Sativum L inductivity is counted after 30 days, as shown in table 1.
Comparative example 4
In the present embodiment, the coat of the garlic petal of aerosol index and false stem are removed, remove single aerosol index, is removed single
Aerosol index, it is spare；Then by the aerosol index got ready in GA3When immersion 12 ~ 24 is small in solution, after rinsing 7 min with flowing water
It is transferred in superclean bench, 30 s of alcohol disinfecting, aseptic water washing 2 times, 15 min of hypochlorite disinfectant finally uses sterile water
It rinses 5 times.The cultivation of processed garlic aerosol index is grown naturally in nursery afterwards.Allium Sativum L is counted after 30 days to lure
Conductance, as shown in table 1.
Comparative example 5
The present embodiment provides a kind of abductive approach of garlic test tube seedling, the method carries out in accordance with the following steps：
S01：The preparation of culture medium：Using MS culture mediums as minimal medium, 30 g of sucrose, 7 g of agar are added in 1 L culture mediums,
It boils and dissolves agar, culture medium is settled to 1000 ml, pH value is adjusted to 5.8, is dispensed into tissue culture bottle, every bottle of 30 ml culture
Base, lid lid sealing, 121 DEG C of autoclave sterilization 20 min are spare.
S02：Aerosol index processing：The coat and false stem that the garlic petal of aerosol index will have been induced remove, and remove
Single aerosol index, it is spare；Then by the aerosol index got ready in GA3When immersion 16 is small in solution, after rinsing 7 min with flowing water
It is transferred in superclean bench, 30 s of alcohol disinfecting, aseptic water washing 2 times, 15 min of hypochlorite disinfectant finally uses sterile water
It rinses 5 times.
S03：Inoculation：On superclean bench, with the blotting paper after sterilizing by the surface moisture of the aerosol index sterilized
It blots, aerosol index is inoculated into the culture medium prepared in the step S01 with the tweezers after sterilizing, be transferred to the training of tissue culture room
It supports, illumination condition is 2600 lx, 16 h d-1, room temperature is 25 DEG C.Culture counts Allium Sativum L inductivity after 30 days, such as table 1
It is shown.
Table 1：Each embodiment Allium Sativum L inductivity comparison
| Embodiment | Allium Sativum L inductivity |
| Embodiment 1 | 89.41% |
| Embodiment 2 | 87.22% |
| Embodiment 3 | 87.34% |
| Embodiment 4 | 90.34% |
| Comparative example 1 | 52.02% |
| Comparative example 2 | 34.80% |
| Comparative example 3 | 0 |
| Comparative example 4 | 0.95% |
| Comparative example 5 | 28.56% |
From upper table 1, in method provided in an embodiment of the present invention, after 30 days Allium Sativum L inductivity be significantly larger than comparative example 1,
Comparative example 2, comparative example 3 and comparative example 4, when illustrating that method provided in an embodiment of the present invention can greatly shorten the rudiment of garlic
Between.And laboratory tissue culture method provided by the invention, than being directly seeded in field with higher inductivity, can faster sprout
Bud.
Certainly, above description is also not limited to the example above, the technical characteristic of the invention without description can by or
It is realized using the prior art, details are not described herein；It is not to this that above example, which is merely to illustrate technical scheme,
The limitation of invention is described in detail the present invention with reference to preferred embodiment, and those of ordinary skill in the art should
Understand, the variations, modifications, additions or substitutions that those skilled in the art are made in the essential scope of the present invention
Without departure from spirit of the invention, it should also belong to claims of the invention.
Claims (4)
1. a kind of abductive approach of garlic test tube seedling, which is characterized in that described method includes following steps：
S01：The preparation of culture medium：Using MS culture mediums as minimal medium, add in sucrose, agar, plant growth regulator NAA and
6-BA boils and dissolves agar, and pH value is adjusted to 5.8~6.0, is dispensed into tissue culture bottle, lid lid sealing, autoclave sterilization standby
With；
S02：Aerosol index processing：By the aerosol index got ready in GA3Flowing water rinses after being impregnated in solution, is then transferred to ultra-clean
Alcohol disinfecting, aseptic water washing, hypochlorite disinfectant are carried out in workbench, finally with spare after aseptic water washing；
S03：Inoculation：On superclean bench, the surface moisture for the aerosol index handled well is blotted with the blotting paper after sterilizing,
Aerosol index is inoculated into the culture medium prepared in the step S01 with the tweezers after sterilizing, is transferred to the culture of tissue culture room, light
It is 2600 lx, 16 hd according to condition-1, room temperature is 23~27 DEG C.
2. a kind of abductive approach of garlic test tube seedling according to claim 1, which is characterized in that in the step S01, with
MS culture mediums are minimal medium, and 30 g of sucrose, 7 g of agar are added in 1 L culture mediums, and adds plant growth regulator
The 6-BA of the NAA and 0.3mg/L of 0.05mg/L, boil and dissolve agar, and culture medium is settled to 1000 ml, and pH value is adjusted to 5.8~
6.0, it is dispensed into tissue culture bottle, every bottle of 30 ml culture mediums, lid lid sealing, it is spare after 121 DEG C of 20 min of autoclave sterilization.
3. the abductive approach of a kind of garlic test tube seedling according to claim 1, which is characterized in that, will in the step S02
The aerosol index got ready is in the GA of 100~150mg/L3When immersion 12~24 is small in solution, after then flowing water rinses 5~10 min
It is transferred in superclean bench, 30~60 s of alcohol disinfecting, aseptic water washing 2~3 times, 15~20 min of hypochlorite disinfectant,
Finally use aseptic water washing 3~5 times, it is spare.
4. a kind of abductive approach of garlic test tube seedling according to claim 1, which is characterized in that in the step S02, institute
Stating the aerosol index got ready is：
The coat and false stem that the garlic petal of aerosol index will have been induced remove, the single aerosol index of stripping.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109526743A (en) * | 2018-12-28 | 2019-03-29 | 中国农业科学院蔬菜花卉研究所 | The fast numerous and stock breeding method of garlic callus |
| CN110786216A (en) * | 2019-11-27 | 2020-02-14 | 中国农业科学院烟草研究所 | Garlic aerial bulb direct seeding planting method |
| CN111436372A (en) * | 2019-12-23 | 2020-07-24 | 山东农业大学 | Nutrient solution for inducing garlic aerial bulbs, method and application |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RO96914A2 (en) * | 1986-11-10 | 1989-05-30 | Institutul De Cercetari Pentru Legumicultura Si Floricultura,Ro | RAPID MOVEMENT PROCESS PROCESS |
| CN106508676A (en) * | 2016-11-03 | 2017-03-22 | 广西玉林容山堂生物科技有限公司 | Purification and rejuvenation method for Yulin fragrant garlic |
| CN107432245A (en) * | 2017-07-25 | 2017-12-05 | 山东农业大学 | A kind of garlic aerosol index abductive approach |
-
2018
- 2018-01-09 CN CN201810016835.4A patent/CN108040882A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RO96914A2 (en) * | 1986-11-10 | 1989-05-30 | Institutul De Cercetari Pentru Legumicultura Si Floricultura,Ro | RAPID MOVEMENT PROCESS PROCESS |
| CN106508676A (en) * | 2016-11-03 | 2017-03-22 | 广西玉林容山堂生物科技有限公司 | Purification and rejuvenation method for Yulin fragrant garlic |
| CN107432245A (en) * | 2017-07-25 | 2017-12-05 | 山东农业大学 | A kind of garlic aerosol index abductive approach |
Non-Patent Citations (2)
| Title |
|---|
| 王珍等: "大蒜气生鳞茎快繁体系研究", 《北方园艺》 * |
| 肖杰等: "打破大蒜蒜种休眠的方法与效果", 《湖南农业大学学报（自然科学版）》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109526743A (en) * | 2018-12-28 | 2019-03-29 | 中国农业科学院蔬菜花卉研究所 | The fast numerous and stock breeding method of garlic callus |
| CN110786216A (en) * | 2019-11-27 | 2020-02-14 | 中国农业科学院烟草研究所 | Garlic aerial bulb direct seeding planting method |
| CN111436372A (en) * | 2019-12-23 | 2020-07-24 | 山东农业大学 | Nutrient solution for inducing garlic aerial bulbs, method and application |
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Application publication date: 20180518 |