CN107432245B - A kind of garlic aerosol index abductive approach - Google Patents
A kind of garlic aerosol index abductive approach Download PDFInfo
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- CN107432245B CN107432245B CN201710611010.2A CN201710611010A CN107432245B CN 107432245 B CN107432245 B CN 107432245B CN 201710611010 A CN201710611010 A CN 201710611010A CN 107432245 B CN107432245 B CN 107432245B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Engineering & Computer Science (AREA)
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Abstract
The present invention relates to a kind of garlic aerosol index abductive approach, this method carries out in accordance with the following steps: (1) garlic stems picking and processing: using sterile tool clip garlic stems petal section, and disinfect in alcohol rear spare；(2) prepare culture medium: culture medium is liquid MS medium, sucrose 30g is added in every liter of culture medium, and PH is adjusted to 5.8, spare then through autoclave sterilization 20min；(3) disinfection treatment of garlic stems petal section: the garlic stems petal section of clip is successively subjected to flowing water and rinses 1 ~ 2h, alcohol disinfecting 30s, aseptic water washing 2 ~ 3 times, hypochlorite disinfectant 35min, is finally used aseptic water washing 3 ~ 5 times；(4) it cultivates: the bottom of the garlic stems petal section of disinfection treatment is cut off, and remainder is put into the culture medium prepared in step (2) and is cultivated, illumination condition is 2600 lx, 16 hd-1 when culture, room temperature is (25 ± 2) DEG C, and incubation time is three weeks.
Description
Technical field
The present invention relates to Garlic Tissue cultures and fast numerous method, belong to agro-biological engineering technical field, specially one
Kind garlic aerosol index abductive approach.
Background technique
Garlic (Allium sativum L.) is asexually propagated crop, and since bulb breeding is used for a long time, virus is by generation product
It is tired, along with the influence of unsuitable environmental condition, bad cultivation step, causes garlic " degeneration " phenomenon serious, cause pole to production
Big loss.To avoid continuing for such phenomenon, there is garlic aerosol index (day garlic) breeding, when aerosol index (day garlic) is bred,
Its garlic picks up from the petal (growing point) on garlic stems (flower a kind of sedge) top, and Garlic Growth point position is generally without virus, thus can be natural
Detoxification.Garlic aerosol index is mainly used to purification and rejuvenation in production practices, but just due to only mature aerosol index
It can do " seed ", and the nutrition of ground garlic can be fought for by cultivating mature aerosol index, influence the yield of ground garlic, reduce the receipts of kind of garlic
Benefit.
Summary of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of garlic aerosol index abductive approach, utilize fresh garlic stems
Aerosol index is induced as explant, does not influence the normal growth of ground garlic, and realizes the detoxification of garlic and fast numerous.
The present invention is achieved through the following technical solutions, provides a kind of garlic aerosol index abductive approach, the induction side
Method carries out in accordance with the following steps:
(1) garlic stems picking and processing: sterile tool clip garlic stems petal section is used, and is disinfected in alcohol rear spare；
(2) prepare culture medium: culture medium is liquid MS medium, sucrose 30g is added in every liter of culture medium, and PH is adjusted to
5.8, it is spare then through autoclave sterilization 20min；
(3) disinfection treatment of garlic stems petal section: the garlic stems petal section of clip is successively subjected to 1 ~ 2h of flowing water flushing, alcohol disappears
Malicious 30s, aseptic water washing 2 ~ 3 times, hypochlorite disinfectant 35min are finally used aseptic water washing 3 ~ 5 times；
(4) it cultivates: the bottom of the garlic stems petal section of disinfection treatment being cut off, and remainder is put into step (2)
It is cultivated in the culture medium of preparation, illumination condition is 2 600 lx, 16 h d when culture-1, room temperature is (25 ± 2)
DEG C, incubation time is three weeks.
Preferably, MS culture medium prescription in step (2) are as follows: only contain in every 1L minimal medium
KNO31900mg, NH4NO31650mg,
MgSO4· 7H2O 370 mg, KH2PO4170 mg,
CaCl2· 2H2O 440 mg, MnSO4· 4H222.3 mg of O,
ZnSO4· 7H2O 8.6 mg, H3BO36.2mg
KI 0.83 mg, Na2MoO4·2H20.25 mg of O,
CuSO4· 5H2O 0.025 mg, CoCl2· 6H2O 0.025mg,
Na2- EDTA 37.3 mg, FeSO4· 7H227.8 mg of O,
2.0 mg of glycine, 0.1 mg of thiamine hydrochloride,
0.5 mg of puridoxine hydrochloride, 0.5 mg of niacin,
100 mg of inositol.
Preferably, the garlic stems petal section of the previous day wanted clip of clear water flushing in garlic stems picking.By washing
Except the dust and disease pest of petal section, guarantee going on smoothly when picking.
Preferably, garlic stems picking selection carries out in the morning of fine day.It can be to avoid ambient contamination tool when picking
And petal, to guarantee that picking petal is sterile.
The invention has the benefit that aerosol index is induced by using the tender petal of liquid MS medium culture garlic children,
Each petal can induce aerosol index, so that can reach 100% using the inductivity of this method aerosol index, thus it is real
Show and has carried out fast numerous industrial seedling rearing using garlic aerosol index.
Detailed description of the invention
Fig. 1 is the aerosol index figure before not inducing；
Fig. 2 is the aerosol index figure expanded after inducing；
Specific embodiment
In order to clarify the technical characteristics of the invention, being illustrated below by specific embodiment to this programme.
A kind of garlic aerosol index abductive approach carries out in accordance with the following steps:
One, the picking of garlic garlic stems field and processing
After the garlic bolting of field planting, the picking young garlic shoots when garlic stems is still tender, and aerosol index preliminarily forms but is no mature,
The neither formation rate of influence aerosol index will not fight for the nutrition of ground garlic and influence the income of ground garlic.Picking the previous day uses water
The petal section to be picked is rinsed, to remove dust and disease pest etc., the morning of selection fair weather when picking, with the work after disinfection
Has picking young garlic shoots petal section, it is spare to take back laboratory after disinfecting in alcohol for false stem of each petal with 15-20cm.
Two, culture medium is prepared
The present embodiment used medium is liquid MS medium, adds sucrose 30g in 1L culture medium, is settled to 1000ml,
PH value is adjusted to 5.8, is then dispensed into Boiling tube, and every pipe about 30ml culture medium is sealed mouth, in 121 DEG C of high temperature and high pressure environments
Lower sterilizing 20min, cooling are spare.
The MS culture medium prescription of the present embodiment are as follows: only contain in every 1L minimal medium
KNO31900mg, NH4NO31650mg,
MgSO4· 7H2O 370 mg, KH2PO4170 mg,
CaCl2· 2H2O 440 mg, MnSO4· 4H222.3 mg of O,
ZnSO4· 7H2O 8.6 mg, H3BO36.2mg
KI 0.83 mg, Na2MoO4·2H20.25 mg of O,
CuSO4· 5H2O 0.025 mg, CoCl2· 6H2O 0.025mg,
Na2- EDTA 37.3 mg, FeSO4· 7H227.8 mg of O,
2.0 mg of glycine, 0.1 mg of thiamine hydrochloride,
0.5 mg of puridoxine hydrochloride, 0.5 mg of niacin,
100 mg of inositol
Three, the disinfection treatment of garlic stems petal section
The garlic stems petal section of clip is taken back into laboratory, after rinsing 1 ~ 2h under flowing water, is transferred in superclean bench, and
It successively disinfects 30s, aseptic water washing 2 ~ 3 times, hypochlorite disinfectant 35min in alcohol, finally uses aseptic water washing 3 ~ 5
It is secondary, finally surface water is blotted with blotting paper, it is spare.
Four, the Fiber differentiation of test tube bulbs
The bottom of the garlic stems petal section of disinfection treatment is cut off, and remainder is put into the MS culture medium prepared
It is cultivated, illumination condition is 2 600 lx, 16 h d when culture-1, room temperature is (25 ± 2) DEG C, incubation time three
In week, petal under the nutrition that culture medium provides, expand by growth, curing, aerosol index maturation, is originally crowded with inside tender petal
Full aerosol index.
Through actual count, the present invention induces 100, garlic petal, each petal has aerosol index to derive, and lures
Conductance is 100%.
Certainly, above description is also not limited to the example above, technical characteristic of the present invention without description can by or
It is realized using the prior art, details are not described herein；Above embodiments and attached drawing are only used to illustrate the technical scheme of the present invention not
It is limitation of the present invention, is described the invention in detail referring to preferred embodiment, the ordinary skill people of this field
Member is it should be appreciated that variation, remodeling, the addition that those skilled in the art are made within the essential scope of the present invention
Or replacement also should belong to claims of the invention without departure from spirit of the invention.
Claims (1)
1. a kind of garlic aerosol index abductive approach, which is characterized in that carry out in accordance with the following steps:
(1) picking of garlic garlic stems field and processing:
After the garlic bolting of field planting, the picking young garlic shoots when garlic stems is still tender, and aerosol index preliminarily forms but is no mature；Picking
The previous day is rinsed with water the petal section to be picked, and to remove dust and disease pest, the morning of fair weather is selected when picking, with disappearing
Tool picking young garlic shoots petal section after poison, false stem of each petal with 15-20cm disinfect in alcohol rear spare；
(2) culture medium is prepared:
Using liquid MS medium, adds sucrose 30g in 1L culture medium, be settled to 1000ml, pH value is adjusted to 5.8, is then dispensed into
In Boiling tube, every pipe 30ml culture medium seals mouth, and sterilize 20min under 121 DEG C of high temperature and high pressure environments, and cooling is spare；
The MS culture medium prescription are as follows: only contain in every 1L minimal medium
KNO31900mg, NH4NO31650mg,
MgSO4· 7H2O 370 mg, KH2PO4170 mg,
CaCl2· 2H2O 440 mg, MnSO4· 4H222.3 mg of O,
ZnSO4· 7H2O 8.6 mg, H3BO36.2mg
KI 0.83 mg, Na2MoO4·2H20.25 mg of O,
CuSO4· 5H2O 0.025 mg, CoCl2· 6H2O 0.025mg,
Na2- EDTA 37.3 mg, FeSO4· 7H227.8 mg of O,
2.0 mg of glycine, 0.1 mg of thiamine hydrochloride,
0.5 mg of puridoxine hydrochloride, 0.5 mg of niacin,
100 mg of inositol；
(3) disinfection treatment of garlic stems petal section:
After the garlic stems petal section of clip is rinsed 1-2h under flowing water, it is transferred in superclean bench, and successively disinfect in alcohol
30s, aseptic water washing 2-3 times, hypochlorite disinfectant 35min, aseptic water washing 3-5 times, are finally inhaled surface water with blotting paper
It is dry, it is spare；
(4) Fiber differentiation of test tube bulbs:
The bottom of the garlic stems petal section of disinfection treatment is cut off, and remainder is put into the MS culture medium prepared and is carried out
Culture, illumination condition is 2600lx, 16hd when culture-1, room temperature is 25 DEG C ± 2 DEG C, and incubation time is three weeks, and petal is being cultivated
Under the nutrition that base provides, growth, curing, aerosol index maturation are expanded, and are originally crowded with full aerosol index in tender petal.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108040882A (en) * | 2018-01-09 | 2018-05-18 | 山东农业大学 | A kind of abductive approach of garlic test tube seedling |
| CN110786216A (en) * | 2019-11-27 | 2020-02-14 | 中国农业科学院烟草研究所 | Garlic aerial bulb direct seeding planting method |
| CN111436372A (en) * | 2019-12-23 | 2020-07-24 | 山东农业大学 | Nutrient solution for inducing garlic aerial bulbs, method and application |
| CN111374054A (en) * | 2020-04-11 | 2020-07-07 | 中国农业科学院烟草研究所 | Method for culturing garlic aerial bulbs by using in-vitro young garlic shoots |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102792887A (en) * | 2012-07-27 | 2012-11-28 | 天津师范大学 | Method for inducing garlic test tube bulb |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102792887A (en) * | 2012-07-27 | 2012-11-28 | 天津师范大学 | Method for inducing garlic test tube bulb |
Non-Patent Citations (1)
| Title |
|---|
| 大蒜花序轴离体培养产生鳞茎方法的研究;宋淑敏等;《黑龙江八一农垦大学学报》;20120430;第24卷(第2期);摘要，第1.2.1-1.2.4节 |
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