CN107432245A - A kind of garlic aerosol index abductive approach - Google Patents
A kind of garlic aerosol index abductive approach Download PDFInfo
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- CN107432245A CN107432245A CN201710611010.2A CN201710611010A CN107432245A CN 107432245 A CN107432245 A CN 107432245A CN 201710611010 A CN201710611010 A CN 201710611010A CN 107432245 A CN107432245 A CN 107432245A
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- garlic
- garlic stems
- culture medium
- stems
- petal section
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
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Abstract
The present invention relates to a kind of garlic aerosol index abductive approach, this method is carried out in accordance with the following steps：（1）Garlic stems is plucked and processing：Using sterile instrument clip garlic stems petal section, and disinfect in alcohol rear standby；（2）Prepare culture medium：Culture medium is liquid MS medium, and sucrose 30g is added in every liter of culture medium, and PH is adjusted into 5.8, standby then through autoclave sterilization 20min；（3）Garlic stems petal section is disinfected：The garlic stems petal section of clip is subjected to flowing water successively and rinses 1 ~ 2h, alcohol disinfecting 30s, aseptic water washing 2 ~ 3 times, hypochlorite disinfectant 35min, finally with aseptic water washing 3 ~ 5 times；（4）Culture：The bottom for the garlic stems petal section disinfected is cut off, and remainder is put into step（2）Cultivated in the culture medium of middle preparation, illumination condition is 2600 lx during culture, 16 hd 1, and room temperature is（25±2）DEG C, incubation time is three weeks.
Description
Technical field
The present invention relates to Garlic Tissue culture and fast numerous method, belong to agro-biological engineering technical field, specially one
Kind garlic aerosol index abductive approach.
Background technology
Garlic（Allium sativum L.）For asexually propagated crop, because long-term use of bulb is bred, virus is by generation product
It is tired, along with the influence of unsuitable environmental condition, bad cultivation step, cause garlic " degeneration " phenomenon serious, pole is caused to production
Big loss.To avoid continuing for such a phenomenon, there is garlic aerosol index（Its garlic）Breeding, aerosol index（Its garlic）During breeding,
Its garlic picks up from garlic stems（Flower a kind of sedge）The petal on top（Growing point）, and Garlic Growth point position is typically without virus, thus can be natural
Detoxification.Garlic aerosol index is mainly used to purification and rejuvenation in production practices, but due to only ripe aerosol index
It can do " seed ", and the nutrition of ground garlic can be fought for by cultivating ripe aerosol index, influence the yield of ground garlic, reduce the receipts of kind of garlic
Benefit.
The content of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of garlic aerosol index abductive approach, fresh garlic stems is utilized
Aerosol index is induced as explant, does not influence the normal growth of ground garlic, and realizes the detoxification of garlic and fast numerous.
The present invention is achieved through the following technical solutions, there is provided a kind of garlic aerosol index abductive approach, the induction side
Method is carried out in accordance with the following steps：
（1）Garlic stems is plucked and processing：Using sterile instrument clip garlic stems petal section, and disinfect in alcohol rear standby；
（2）Prepare culture medium：Culture medium is liquid MS medium, and sucrose 30g is added in every liter of culture medium, and PH is adjusted into 5.8,
Then it is standby through autoclave sterilization 20min；
（3）Garlic stems petal section is disinfected：The garlic stems petal section of clip is subjected to flowing water successively and rinses 1 ~ 2h, alcohol disinfecting
30s, aseptic water washing 2 ~ 3 times, hypochlorite disinfectant 35min, finally with aseptic water washing 3 ~ 5 times；
（4）Culture：The bottom for the garlic stems petal section disinfected is cut off, and remainder is put into step（2）Middle preparation
Culture medium in cultivated, illumination condition is 2 600 lx during culture, 16 h d-1, and room temperature is（25 ± 2）DEG C, training
It is three weeks to support the time.
Preferably, step（2）Middle MS culture medium prescriptions are：Contain in per 1L minimal mediums
KNO3 1900mg, NH4NO3 1650mg,
The mg of MgSO4 7,H20 370 mg, KH2PO 4170,
The mg of CaCl2 2,H20 440 mg, MnSO4 4,H20 22.3,
ZnSO4 7,H20 8.6 mg, H3BO3 6.2mg,
The mg of KI 0.83 mg, Na2MO3 2,H20 0.25,
CuSO4 5,H20 0.025 mg, CoCl2 6H20 0.025mg,
The mg of Na2-EDTA 37.3 mg, FeSO4 7,H20 27.8,
The mg of glycine 2.0, the mg of thiamine hydrochloride 0.1,
The mg of puridoxine hydrochloride 0.5, the mg of nicotinic acid 0.5,
The mg of inositol 100.
Preferably, the garlic stems petal section for wanting clip is rinsed with clear water in the previous day of garlic stems harvesting.By washing
Except the dust and disease pest of petal section, ensure to be smoothed out during harvesting.
Preferably, garlic stems harvesting selection is carried out in the morning of fine day.Ambient contamination instrument when can avoid plucking
And petal, to ensure that harvesting petal is sterile.
Beneficial effects of the present invention are：Aerosol index is induced by using the tender petal of liquid MS medium culture garlic children,
Each petal can induce aerosol index so that 100% is can reach using the inductivity of this method aerosol index, thus it is real
Show and carried out fast numerous industrial seedling rearing using garlic aerosol index.
Brief description of the drawings
Fig. 1 is the aerosol index figure before not inducing；
Fig. 2 is the aerosol index figure expanded after inducing.
Embodiment
For the technical characterstic for illustrating this programme can be understood, below by embodiment, this programme is illustrated.
A kind of garlic aerosol index abductive approach, is carried out in accordance with the following steps：
First, the harvesting of garlic garlic stems field and processing
After the garlic bolting of field planting, the picking young garlic shoots when garlic stems is still tender, and aerosol index preliminarily forms but unripe, neither
The formation rate of aerosol index is influenceed, the nutrition of ground garlic will not be also fought for and influence the income of ground garlic.Harvesting the previous day is rinsed with water
The petal section to be plucked, the morning of fair weather is selected to remove dust and disease pest etc., during harvesting, is adopted with the instrument after sterilization
Garlic stems petal section is plucked, false stem of each petal with 15-20cm, it is standby to take back laboratory after disinfecting in alcohol.
2nd, culture medium is prepared
The present embodiment used medium is liquid MS medium, adds sucrose 30g in 1L culture mediums, is settled to 1000ml, pH value
5.8 are adjusted to, is then dispensed into Boiling tube, often pipe about 30ml culture mediums, seal mouth, are gone out under 121 DEG C of high temperature and high pressure environments
Bacterium 20min, cooling are standby.
The MS culture medium prescriptions of the present embodiment are：Contain in per 1L minimal mediums
KNO3 1900mg, NH4NO3 1650mg,
The mg of MgSO4 7,H20 370 mg, KH2PO 4170,
The mg of CaCl2 2,H20 440 mg, MnSO4 4,H20 22.3,
ZnSO4 7,H20 8.6 mg, H3BO3 6.2mg,
The mg of KI 0.83 mg, Na2MO3 2,H20 0.25,
CuSO4 5,H20 0.025 mg, CoCl2 6H20 0.025mg,
The mg of Na2-EDTA 37.3 mg, FeSO4 7,H20 27.8,
The mg of glycine 2.0, the mg of thiamine hydrochloride 0.1,
The mg of puridoxine hydrochloride 0.5, the mg of nicotinic acid 0.5,
The mg of inositol 100
3rd, garlic stems petal section is disinfected
The garlic stems petal section of clip is taken back into laboratory, after rinsing 1 ~ 2h under flowing water, is transferred in superclean bench, and successively
Disinfect 30s, aseptic water washing 2 ~ 3 times, hypochlorite disinfectant 35min in alcohol, finally with aseptic water washing 3 ~ 5 times, most
Surface water is blotted with blotting paper afterwards, it is standby.
4th, the Fiber differentiation of test tube bulbs
The bottom for the garlic stems petal section disinfected is cut off, and remainder is put into the MS culture mediums prepared and carried out
Culture, illumination condition is 2 600 lx during culture, 16 h d-1, and room temperature is（25 ± 2）DEG C, incubation time is three weeks,
Petal under the nutrition that culture medium provides, expand, and is originally crowded with inside tender petal by growth, curing, aerosol index maturation
Full aerosol index.
Through actual count, 100, present invention induction garlic petal, each petal has aerosol index to derive, and lures
Conductance is 100%.
Certainly, described above is also not limited to the example above, the technical characteristic of the invention without description can by or
Realized, will not be repeated here using prior art；Above example and accompanying drawing are merely to illustrate technical scheme
It is limitation of the present invention, the present invention is described in detail with reference to preferred embodiment, the ordinary skill people of this area
Member is it should be appreciated that change, remodeling, the addition that those skilled in the art are made in the essential scope of the present invention
Or replace the claims that without departure from spirit of the invention, should also belong to the present invention.
Claims (4)
1. a kind of garlic aerosol index abductive approach, it is characterised in that carry out in accordance with the following steps：
（1）Garlic stems is plucked and processing：Using sterile instrument clip garlic stems petal section, and disinfect in alcohol rear standby；
（2）Prepare culture medium：Culture medium is liquid MS medium, and sucrose 30g is added in every liter of culture medium, and PH is adjusted into 5.8,
Then it is standby through autoclave sterilization 20min；
（3）Garlic stems petal section is disinfected：The garlic stems petal section of clip is subjected to flowing water successively and rinses 1 ~ 2h, alcohol disinfecting
30s, aseptic water washing 2 ~ 3 times, hypochlorite disinfectant 35min, finally with aseptic water washing 3 ~ 5 times；
（4）Culture：The bottom for the garlic stems petal section disinfected is cut off, and remainder is put into step（2）Middle preparation
Culture medium in cultivated, illumination condition is 2 600 lx during culture, 16 h d-1, and room temperature is（25 ± 2）DEG C, training
It is three weeks to support the time.
A kind of 2. garlic aerosol index abductive approach according to claim 1, it is characterised in that：Step（2）Middle MS cultures
Based formulas is：Contain in per 1L minimal mediums
KNO3 1900mg, NH4NO3 1650mg,
The mg of MgSO4 7,H20 370 mg, KH2PO 4170,
The mg of CaCl2 2,H20 440 mg, MnSO4 4,H20 22.3,
ZnSO4 7,H20 8.6 mg, H3BO3 6.2mg,
The mg of KI 0.83 mg, Na2MO3 2,H20 0.25,
CuSO4 5,H20 0.025 mg, CoCl2 6H20 0.025mg,
The mg of Na2-EDTA 37.3 mg, FeSO4 7,H20 27.8,
The mg of glycine 2.0, the mg of thiamine hydrochloride 0.1,
The mg of puridoxine hydrochloride 0.5, the mg of nicotinic acid 0.5,
The mg of inositol 100.
A kind of 3. garlic aerosol index abductive approach according to claim 1, it is characterised in that：In the previous of garlic stems harvesting
It is rinsed with clear water and wants clip garlic stems petal section.
A kind of 4. garlic aerosol index abductive approach according to claim 1, it is characterised in that：Garlic stems harvesting selection is fine
It the morning is carried out.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710611010.2A CN107432245B (en) | 2017-07-25 | 2017-07-25 | A kind of garlic aerosol index abductive approach |
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| CN201710611010.2A CN107432245B (en) | 2017-07-25 | 2017-07-25 | A kind of garlic aerosol index abductive approach |
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| CN107432245A true CN107432245A (en) | 2017-12-05 |
| CN107432245B CN107432245B (en) | 2019-08-06 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108040882A (en) * | 2018-01-09 | 2018-05-18 | 山东农业大学 | A kind of abductive approach of garlic test tube seedling |
| CN110786216A (en) * | 2019-11-27 | 2020-02-14 | 中国农业科学院烟草研究所 | Garlic aerial bulb direct seeding planting method |
| CN111374054A (en) * | 2020-04-11 | 2020-07-07 | 中国农业科学院烟草研究所 | Method for culturing garlic aerial bulbs by using in-vitro young garlic shoots |
| CN111436372A (en) * | 2019-12-23 | 2020-07-24 | 山东农业大学 | Nutrient solution for inducing garlic aerial bulbs, method and application |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102792887A (en) * | 2012-07-27 | 2012-11-28 | 天津师范大学 | Method for inducing garlic test tube bulb |
-
2017
- 2017-07-25 CN CN201710611010.2A patent/CN107432245B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102792887A (en) * | 2012-07-27 | 2012-11-28 | 天津师范大学 | Method for inducing garlic test tube bulb |
Non-Patent Citations (1)
| Title |
|---|
| 宋淑敏等: "大蒜花序轴离体培养产生鳞茎方法的研究", 《黑龙江八一农垦大学学报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108040882A (en) * | 2018-01-09 | 2018-05-18 | 山东农业大学 | A kind of abductive approach of garlic test tube seedling |
| CN110786216A (en) * | 2019-11-27 | 2020-02-14 | 中国农业科学院烟草研究所 | Garlic aerial bulb direct seeding planting method |
| CN111436372A (en) * | 2019-12-23 | 2020-07-24 | 山东农业大学 | Nutrient solution for inducing garlic aerial bulbs, method and application |
| CN111374054A (en) * | 2020-04-11 | 2020-07-07 | 中国农业科学院烟草研究所 | Method for culturing garlic aerial bulbs by using in-vitro young garlic shoots |
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| CN107432245B (en) | 2019-08-06 |
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