CN108040678A - A kind of device and its application for dividing root earth culture for plant - Google Patents

A kind of device and its application for dividing root earth culture for plant Download PDF

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Publication number
CN108040678A
CN108040678A CN201711091623.4A CN201711091623A CN108040678A CN 108040678 A CN108040678 A CN 108040678A CN 201711091623 A CN201711091623 A CN 201711091623A CN 108040678 A CN108040678 A CN 108040678A
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China
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root
plant
incubator
field planting
soil
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CN201711091623.4A
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Chinese (zh)
Inventor
张承林
涂攀峰
姬静华
邓兰生
陈杰忠
程凤娴
官利兰
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South China Agricultural University
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South China Agricultural University
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Priority to CN201711091623.4A priority Critical patent/CN108040678A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/02Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Botany (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)

Abstract

The invention belongs to field of plant growing technology, specifically disclose a kind of device for dividing root earth culture for plant, including incubator, partition plate, field planting hole, field planting sponge and water seepage hole;The incubator center is equipped with several vertical partition plates, and incubator is separated into several uniform small casees;Field planting hole is equipped at the top of the intersection of several partition plates, is equipped with to fix the field planting sponge of plant seedlings above field planting hole;The bottom of the incubator is equipped with water seepage hole.Described device can be applied to influence of the research Different Nutrients supply to plant root growth.The device can preferably fix plant seedlings on the apparatus, and can divide equally plant seedlings root system, and reduction divides injury degree of the root to seedlings root, and raising divides the survival rate of seedling after root.The device sets advantages of simple, easy to operate, is suitable for large-scale promotion and application.

Description

A kind of device and its application for dividing root earth culture for plant
Technical field
The present invention relates to field of plant growing technology, and in particular, to it is a kind of for plant divide root earth culture device and its Using.
Background technology
In nature, the nutrient in soil is usually all soil existing for heterogeneous state on spatio-temporal distribution The heterogeneity of earth nutrient is mainly due to the uneven of sedimentation, plant defoliation and other organic substances input and is subject to micro- in soil What the decomposition of biology generated during nature long-term evolution.Meanwhile in farmland ecosystem, either water-fertilizer integral The fertilization modes such as the spreading manuer in holes of sprinkling irrigation, trickle irrigation or peasant convention in technology, ditch spread, belong to partial fertilizer, exacerbate soil The heterogeneous distribution of middle nutrient, forms Nutrient patches.
Moisture, nutrient and the various environmental signals that growing plants root system is touched in soil not exclusively, plant It can be largely proliferated in the region with the ability that root system is stretched into eutrophication region, especially lateral root, illustrate local nutrient Supply affects distribution situation of the root system in the soil body.In order to meet the different demands of different root systems, it is necessary to carry out a point root and plant Training, by the way that the root system of same plant is separately planted in the soil body of Different Nutrients content, research root system is heterogeneous to nutrient to be divided The response of cloth.
It is that a kind of simulation root system is in Different Nutrition state to divide root experiment, and root system life is studied under overground part unanimous circumstances Long effective ways.Traditional split-root cultivation device is that plant is fixed on above one piece of culture plate, and incubator is divided into partition plate Two parts, but plant seedlings cannot be fixed well.And the root-planting method that plant main root is rived, not only to plant roots The injury of system is bigger, is also less prone to make root system mean allocation, causes that the seedling percent after point root is relatively low and test effect is poor. Not yet occur simultaneously fixing plant seedlings in currently available technology, plant seedlings root system is divided equally, is not injured The plant of root system divides root earth culture device.
The content of the invention
The present invention in order to overcome the above-mentioned deficiency of the prior art, provide it is a kind of for plant divide root earth culture device and its should With it is many that the device can be applied to plant soil type, different fertilizer species, Different Fertilization amount and water and fertilizer coupling etc. Research.
To achieve these goals, the present invention is achieved by following scheme:
A kind of device for dividing root earth culture for plant, which is characterized in that including incubator 1, partition plate 2, field planting hole 3, field planting sponge 4 With water seepage hole 5;1 center of incubator is equipped with several vertical partition plates 2, and it is uniform small that incubator 1 is separated into several Case;Field planting hole 3 is equipped at the top of the intersection of several partition plates 2,3 top of field planting hole is equipped with to fix determining for plant seedlings Plant sponge 4;The bottom of the incubator 1 is equipped with water seepage hole 5.
The device of the present invention for dividing root earth culture for plant is equipped with several vertical partition plates 2 in 1 center of incubator, Incubator 1 can be separated into several uniform small casees, if for dividing root that the lateral root of plant is divided into cadre when cultivating Point, it plants cultivated in each small case respectively;Field planting hole 3 is equipped at the top of the intersection of several partition plates 2, for dividing root The main root of plant is inserted into field planting hole 3 during cultivation and carries out field planting culture;Meanwhile 3 top of field planting hole is equipped with and is planted for fixed The field planting sponge 4 of object seedling;The field planting hole 3 of device provided by the present invention and field planting sponge 4 can reduce point root to seedlings root Injury degree, while can preferably by plant fix on the apparatus.
Preferably, the incubator 1 is made of transparent material, facilitates observation.
Preferably, the incubator 1 detachably assembles.
Preferably, the height of the partition plate 2 is less than incubator 1.
Preferably, the thickness of the partition plate 2 is 15mm, and the setting of top both sides is angled 0~60 degree of inclined-plane 6, is convenient for Fibrous root is inserted vertically into the soil body as far as possible.
Preferably, the gap 7 that vertical width is 2~3mm is equipped among the partition plate 2, so as to prevent being divided by incubator 1 The soil nutrients of several small casees interpenetrated in water drenching with water body, prevent nutrient from intersecting.
Preferably, the field planting hole 3 is circle, and a diameter of 3~4mm, depth is 25~30mm, convenient for consolidating for plant seedlings It is fixed.
Preferably, a diameter of 8~10mm of the water seepage hole 5.
It is preferably, described for plant the device of root earth culture to be divided to further include to wrap up the black thin film outside incubator 1, The black thin film can be wrapped in outside incubator 1, so as to prevent the influence to root growth such as ambient light photograph, temperature.
Preferably, the incubator 1 is made of thickness of the transparent organic glass of 15~30mm.
Preferably, the partition plate 2 is identical with the making material of incubator 1.
Preferably, it is connected between 1 each side of incubator by screw, corresponding side can be removed by opening screw Face is conducive to directly observe root system and carries out root system sampling, while convenient for the cleaning of incubator 1.
Preferably, the shape of the incubator 1 can also be the shape of the suitable specific test requirements documents such as ellipse, circle.
It is a kind of to carry out a point cultural method for root earth culture using any of the above-described described device, include the following steps:
S1. into several small casees of incubator 1 be packed into etc. weight wind desiceted soil;
S2. water drenching, the soil in each small case is fully drenched;
S3. plant seedlings are taken, its main root is retained after 1.5~2.5cm cuts lower part and is inserted into field planting hole 3, fibrous root is average If being divided into stem portion, plant cultivated in each small case of S2 respectively;
S4. one piece of field planting sponge 4 is placed around seedling, and in incubator 1 outer wrapping, one layer of black thin film.
Any of the above-described described device, which is also claimed, in the present invention influences plant root growth in research Different Nutrients supply Application.
The application specifically includes following steps:
S1. into several small casees of incubator 1 be packed into etc. weight wind desiceted soil;
S2. water drenching, the soil in each small case is fully drenched;
S3. plant seedlings are taken, its main root is retained after 1.5~2.5cm cuts lower part and is inserted into field planting hole 3, fibrous root is average If being divided into stem portion, plant cultivated in each small case of S2 respectively;
S4. one piece of field planting sponge 4 is placed around seedling, and in incubator 1 outer wrapping, one layer of black thin film;
S5. different nutrients is supplied into each small case of S2, Routine Management is carried out to plant seedlings, collected and plant after 3~4 months Object root system simultaneously measures.
Preferably, set there are one vertical partition plate, incubator is separated into two uniform small in incubator 1 described in S1 Case.
Preferably, wind desiceted soil described in S1 is low-phosphorous red soil.
Preferably, plant seedlings described in S3 are sour tangerine seedling.
As it is a kind of explain the invention is intended to embodiment, when research Different Nutrients supply to sour tangerine seedlings root give birth to During long influence, it can pass through and following 5 groups of different nutrient supplies is set to handle respectively in 5 different incubators:
The processing of T1 groups:N P and K is supplied in root case both sides, as control.
The processing of T2 groups:N P and K, opposite side supply nitrogen phosphorus, influence of the research potash fertilizer to root system are supplied in root case one side;
The processing of T3 groups:N P and K, opposite side supply nitrogen potassium, influence of the research phosphate fertilizer to root system are supplied in root case one side;
The processing of T4 groups:N P and K, opposite side supply nitrogen, influence of the research phosphorus potassium complex fertilizer to root system are supplied in root case one side;
The processing of T5 groups:Supply nitrogen phosphorus, opposite side supply nitrogen potassium, research phosphate fertilizer, the potash fertilizer influence to root system respectively in root case one side.
Compared with prior art, the invention has the advantages that:
(1)Device provided by the present invention can preferably fix plant seedlings on the apparatus, and can be by plant seedlings root system Divided equally, reduction divides injury degree of the root to seedlings root, and raising divides the survival rate of seedling after root.
(2)The device interpenetrates between can effectively prevent Different Nutrients with water, and test accuracy is improved, and can answer The overground part and root growth of seedling are dynamically influenced for studying the rich water supply of tree fruit seedling difference rhizosphere region and dosage.
(3)The apparatus structure is simple, easy to operate, flexible, and with detachability, can reuse, suitable for extensively Popularization and application.The device is the partial fertilizer of research field soil, irrigates, and keeps the water supply fertilizer of soil with the development institute of plant It needs rich water harmonious, improves plant liquid fertilizer utilization rate, reduce means of agricultural production input etc. and provide convenience.
(4)The device can simulating plant root soil environmental condition, it may also be used for Observe and measure difference rich water is planted for reply What object root growth influenced divides root to test, and filters out the fertilizer for promoting plant root growth, for the production of regulation and control orchard fruit, improves Fruit quality, reduction orchard input etc. are provided convenience.
Description of the drawings
Fig. 1 is a kind of structure diagram for being used for plant and dividing the device of root earth culture in embodiment 1.
Fig. 2 is a kind of stereogram for being used for plant and dividing the device of root earth culture in embodiment 1.
Fig. 3 is a kind of plan view for being used for plant and dividing the device of root earth culture in embodiment 1.
Fig. 4 is a kind of top view for being used for plant and dividing the device of root earth culture in embodiment 1.
Fig. 5 is a kind of side view for being used for plant and dividing the device of root earth culture in embodiment 1.
Fig. 6 is the lower distribution map in situ of root system in the incubator of Different Nutrients supply in embodiment 2;A is that T1 groups are handled, B For the processing of T2 groups, C is the processing of T3 groups, and D is the processing of T4 groups, and E is the processing of T5 groups.
Caption:1- incubators;2- partition plates;3- field planting holes;4- is colonized sponge;5- water seepage holes;6- inclined-planes;7- gaps.
Specific embodiment
The present invention is made with reference to Figure of description and specific embodiment and further being elaborated, the embodiment It is served only for explaining the present invention, is not intended to limit the scope of the present invention.Test method used in following embodiments is such as without spy Different explanation, is conventional method;Used material, reagent etc., unless otherwise specified, for the reagent commercially obtained And material.
Embodiment 1
As shown in Figure 1, a kind of device for dividing root earth culture for plant is present embodiments provided, including incubator 1, partition plate 2, field planting Hole 3, field planting sponge 4, water seepage hole 5;The incubator 1 is made of thickness of the transparent organic glass of 15mm, and center is equipped with vertical Partition plate 2;The top center of the partition plate 2 is equipped with field planting hole 3, and top is equipped with to fix the field planting sponge 4 of plant seedlings;Institute The bottom for stating incubator 1 is equipped with 2 water seepage holes 5.
The incubator 1 detachably assembles, and is connected between 1 each side of incubator by screw, can be by opening spiral shell Nail, removes corresponding side, is conducive to directly observe root system and carries out root system sampling, while convenient for the cleaning of incubator 1.
As shown in Fig. 2, the volume of the device is:Long 390mm × wide 29mm × high 390mm.
As shown in figure 3, the thickness of partition plate 2 is 15 mm, top is away from 1 open top face of incubator, 28 mm.The top of partition plate 2 The setting of portion both sides is angled 0~60 degree of inclined-plane 6, is inserted vertically into as far as possible in the soil body convenient for fibrous root;The centre of partition plate 2 is set The gap 7 for having vertical width to be 2mm prevents the soil nutrients of two small casees being divided by incubator 1 in water drenching with water body phase Mutually infiltration, prevents nutrient from intersecting.
As shown in Figure 4 and Figure 5, the top center of partition plate 2 is equipped with field planting hole 3, and a diameter of 3mm, depth 25mm are convenient for The fixation of plant seedlings;1 bottom of incubator is equipped with 2 water seepage holes 5, is respectively placed in the bottom of two small casees, the diameter of water seepage hole 5 For 8 mm.
The device further includes to wrap up the black thin film outside incubator 1, and the black thin film can be wrapped in incubator 1 Outside, so as to prevent the influence to root growth such as ambient light photograph, temperature.
The shape of the incubator 1 can also be the shape of the suitable specific test requirements documents such as ellipse, circle.
It is a kind of for plant the device of root earth culture to be divided to carry out a point cultural method for root earth culture using above-mentioned, including walking as follows Suddenly:
S1. into two small casees of incubator 1 be packed into etc. weight wind desiceted soil;
S2. water drenching, the soil in two small casees is fully drenched;
S3. plant seedlings are taken, its main root is retained after 1.5~2.5cm cuts lower part and is inserted into field planting hole 3, fibrous root is average It is divided into two parts to plant respectively in two small casees;
S4. one piece of field planting sponge 4 is placed around seedling, and in incubator 1 outer wrapping, one layer of black thin film.
Embodiment 2
The application that 1 described device of embodiment influences plant root growth in research Different Nutrients supply.
The application specifically includes following steps:
1st, into several small casees of incubator 1 be packed into etc. weight air-dry low-phosphorous red soil;
2nd, water drenching, the soil in each small case is fully drenched;
3rd, size, growing way and the basically identical annual sour tangerine seedling of fibrous root quantity are taken, is inserted into after main root length is cut to 2.5cm In field planting hole 3, fibrous root, which is divided into two parts, plants cultivated in two small casees respectively;
4th, one piece of field planting sponge 4 is placed around seedling, and in incubator 1 outer wrapping, one layer of black thin film;
5th, different nutrients is supplied into each small case of step 2, plant seedlings is carried out with conventional moisture and pest management, Root system of plant is collected after 4 months and measures fresh weight.
As a result as shown in table 1 and Fig. 6, the influence to root system fresh weight:Nitrogen, phosphorus, potash fertilizer supply the root system fresh weight phase of one side It is poor little, and higher than other processing;In T2 processing, both sides root system fresh weight is suitable, illustrates growth effect of the potash fertilizer to sour tangerine root system Less;Phosphate fertilizer one side is not applied in T3, T5 processing and distinguishes low 2.28g, 2.04g compared with opposite side root fresh weight, illustrates that phosphorus is promoting root It plays an important role in system's growth;In T4 processing, only it is notable to apply one side compared with nitrogen, phosphorus and potassium fertilizer for root system fresh weight in nitrogen fertilizer application one side Low 2.58g.
The influence that the sour tangerine partial root zone of table 1 is applied fertilizer to sour tangerine root system fresh weight
Above-mentioned result of the test shows that a certain amount of phosphate fertilizer is supplemented in low-phosphorous red soil can remarkably promote the growth of sour tangerine root system Development.

Claims (8)

1. a kind of device for dividing root earth culture for plant, which is characterized in that including incubator(1), partition plate(2), field planting hole(3)、 It is colonized sponge(4)And water seepage hole(5);The incubator(1)Center is equipped with several vertical partition plates(2), by incubator(1)Point It is divided into several uniform small casees;Several described partition plates(2)Intersection at the top of be equipped with field planting hole(3), field planting hole(3)Top Equipped with for fixing the field planting sponge of plant seedlings(4);The incubator(1)Bottom be equipped with water seepage hole(5).
2. the device according to claim 1 for dividing root earth culture for plant, which is characterized in that the partition plate(2)Thickness For 15mm, the setting of top both sides is angled 0~60 degree of inclined-plane(6), centre is equipped with the gap that vertical width is 2~3mm (7).
3. the device according to claim 1 for dividing root earth culture for plant, which is characterized in that the field planting hole(3)For circle Shape, a diameter of 3~4mm, depth are 25~30mm.
4. a kind of carry out a point cultural method for root earth culture using 3 any described device of claims 1 to 3, include the following steps:
S1. to incubator(1)Several small casees in be packed into etc. weight wind desiceted soil;
S2. water drenching, the soil in each small case is fully drenched;
S3. plant seedlings are taken, its main root is retained after 1.5~2.5cm cuts lower part and is inserted into field planting hole(3)In, fibrous root is put down If being divided into stem portion, plant cultivated in each small case of S2 respectively;
S4. one piece of field planting sponge is placed around seedling(4), and in incubator(1)One layer of black thin film of outer wrapping.
5. application of any one of claims 1 to 3 described device in research Different Nutrients supply influences plant root growth.
6. application according to claim 5, which is characterized in that the application specifically includes following steps:
S1. to incubator(1)Several small casees in be packed into etc. weight wind desiceted soil;
S2. water drenching, the soil in each small case is fully drenched;
S3. plant seedlings are taken, its main root is retained after 1.5~2.5cm cuts lower part and is inserted into field planting hole(3)In, fibrous root is put down If being divided into stem portion, plant cultivated in each small case of S2 respectively;
S4. one piece of field planting sponge is placed around seedling(4), and in incubator(1)One layer of black thin film of outer wrapping;
S5. different nutrients is supplied into each small case of S2, Routine Management is carried out to plant seedlings, collected and plant after 3~4 months Object root system simultaneously measures.
7. application according to claim 5, which is characterized in that wind desiceted soil described in S1 is low-phosphorous red soil.
8. application according to claim 5, which is characterized in that plant seedlings described in S3 are sour tangerine seedling.
CN201711091623.4A 2017-11-08 2017-11-08 A kind of device and its application for dividing root earth culture for plant Pending CN108040678A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110275001A (en) * 2019-07-31 2019-09-24 四川省农业科学院生物技术核技术研究所 It is a kind of to measure and evaluate medicament to the apparatus and method of root-knot nematode drug effect
CN113678659A (en) * 2021-08-30 2021-11-23 河北农业大学 Plant root dividing device based on germination paper and plant root dividing culture method
CN114062599A (en) * 2021-11-04 2022-02-18 西交利物浦大学 Device and method for toxicology research of plant roots
CN114062599B (en) * 2021-11-04 2024-06-07 西交利物浦大学 Device and method for researching toxicology of plant roots

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CN203661756U (en) * 2013-12-16 2014-06-25 广西大学 Aerosol root division culturing tank
CN205794247U (en) * 2016-07-05 2016-12-14 安徽师范大学 The test device that researching plant root interacts
CN206251671U (en) * 2016-09-26 2017-06-16 宿迁学院 A kind of new point of root culture apparatus

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CN101502219A (en) * 2009-03-06 2009-08-12 中国科学院东北地理与农业生态研究所 Split crop root system cultivation device and cultivation method thereof
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110275001A (en) * 2019-07-31 2019-09-24 四川省农业科学院生物技术核技术研究所 It is a kind of to measure and evaluate medicament to the apparatus and method of root-knot nematode drug effect
CN113678659A (en) * 2021-08-30 2021-11-23 河北农业大学 Plant root dividing device based on germination paper and plant root dividing culture method
CN114062599A (en) * 2021-11-04 2022-02-18 西交利物浦大学 Device and method for toxicology research of plant roots
CN114062599B (en) * 2021-11-04 2024-06-07 西交利物浦大学 Device and method for researching toxicology of plant roots

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