CN108030757A - A kind of anti-allergic skin care product containing Japanese Stephania Root stem cell extract - Google Patents
A kind of anti-allergic skin care product containing Japanese Stephania Root stem cell extract Download PDFInfo
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Abstract
The invention discloses a kind of antiallergic composition, the composition contains the component of following parts by weight：Contain 1.1~9.2 parts of 0.07~3.5 part of Japanese Stephania Root stem cell extract, 0.6~6.8 part of phellodendron extract, 0.9~8.3 part of Bitter Orang P.E, 1.3~9.5 parts of Honegsukle flower P.E and Rhizoma Zingiberis zerumbet extract.The skin care item of the present invention contain Japanese Stephania Root stem cell extract, phellodendron extract, Bitter Orang P.E, Honegsukle flower P.E and Rhizoma Zingiberis zerumbet extract, have good antiallergic effect；In addition, the skin care item of the present invention are free of preservative, it is nontoxic to skin safe.
Description
Technical field
The present invention relates to technical field of skin care, especially a kind of antiallergy shield containing Japanese Stephania Root stem cell extract
Skin product.
Background technology
Skin allergy is a kind of very common allergy form, and the people for having 20% has phenomena of cutaneous irritation.Skin allergy is also known as
For allergic skin.One big feature of allergic skin is exactly to itch.Say that skin allergy is primarily referred to as working as skin from medical angle
It is subject to some food of cosmetics, chemicals, pollen, the air of pollution of various stimulation such as adverse reactions, causes skin
Occur it is red and swollen, itch, peel and the skin allergy symptom such as allergic dermatitis.Basic group of studies have shown that color cosmetic or skin care products
The uncomfortable phenomenon that causes skin sensitivity is likely into fragrance matter.
Japanese Stephania Root, alias Radix Stephaniae Cepharanthae, public mouse rattan, wild peach grass, firecracker disappears, towards day ointment, close cymbal grass, spun gold lotus
Leaf, day plaster, are the root or cauline leaf of Menispermaceae stephania plant Japanese Stephania Root；With clearing heat and detoxicating, wind-expelling pain-stopping, Li Shui disappears
The effect of swollen；It is usually used in abscess of throat, carbuncle swells boil, venomous snake bite, arthralgia pain due to rheumatism, stomachache, tinea pedis oedema；Its pharmacological action
Including：1) Muscle relaxation；2) ganglionic blocking action；3) foreign study proves that Japanese Stephania Root has the work of prevention leukopenia
With.
On October 25th, 2010, Nature Biotechnology have delivered the research of the Unhwa Corp companies of South Korea
Institute is successfully separated and has cultivated the paper of plant stem cell first, and has obtained certification, plant to the identity of plant stem cell
The research of stem cell enters the stage of high speed development.
In simple terms, there are two " group " cells for being located at plant tip in plant, they have powerful self-renewing
Ability, can not only be divided into autogenous cell, moreover it is possible to and any other plant cell is divided into, is qualified " general-purpose cell ",
Moreover, they, which also have, continues splitting ability so that many large-scale plant existences more than thousand.
2012, " plant stem cell " technology was studied in the effect of beauty and health care field and has been achieved for certain progress,
Prove metabolism of the plant stem cell to raising skin, improve microcirculation, tightening and recovery skin elasticity have major application
Value.
The content of the invention
Based on this, one kind is provided and can effectively be resisted it is an object of the invention to overcome above-mentioned the deficiencies in the prior art part
The skin care item of skin allergy, the skin care item are safe and non-toxic to skin.
To achieve the above object, this invention takes following technical scheme：
As the first aspect of the invention, the present invention provides a kind of antiallergic composition, the composition contain with
The component of lower parts by weight：Carried containing 0.07~3.5 part of Japanese Stephania Root stem cell extract, 0.6~6.8 part of phellodendron extract, the dried immature fruit of citron orange
Take 1.1~9.2 parts of 0.9~8.3 part of thing, 1.3~9.5 parts of Honegsukle flower P.E and Rhizoma Zingiberis zerumbet extract.
Cortex Phellodendri can be used for damp-heat dysentery, and jaundice, with lower pruritus vulvae, puckery pain of heat gonorrhea, tinea pedis impotence is knitted, and hectic fever due to yin labor heat, steals
Sweat, seminal emission, sore swollen toxin, eczema wet sore, stir-baked CORTEX PHELLODENDRI with salt solution nourishing yin and lessening fire, for fire excess from yin deficiency, night sweat hectic fever due to yin；Report according to the experiment, acid
The orange dried immature fruit of citron orange has lasting boosting, also has improving micro_circulation effect, suffers a shock suitable for treatment；To small after Honeysuckle bine pot water
The pruituses such as child's eczema have certain therapeutic effect, and a variety of to the sick germs, virus to livestock and poultry have inhibitory action；According to the study
Report, the extract of Rhizoma Zingiberis zerumbet have antianaphylactic effect.
Preferably, the mass ratio of the Japanese Stephania Root stem cell extract, phellodendron extract and Rhizoma Zingiberis zerumbet extract is 0.15
~1.8：3：2.7~6.9.It is highly preferred that the Japanese Stephania Root stem cell extract, phellodendron extract and Rhizoma Zingiberis zerumbet extract
Mass ratio is 0.3：3：4.1.Present inventor has found through test of many times, when Japanese Stephania Root stem cell extract in skin care item,
The mass ratio of phellodendron extract and Rhizoma Zingiberis zerumbet extract is 0.15~1.8：3：When 2.7~6.9, the antiallergic effect of skin care item
Preferably；When the mass ratio of Japanese Stephania Root stem cell extract, phellodendron extract and Rhizoma Zingiberis zerumbet extract in skin care item is 0.3：3：
When 4.1, the antiallergic effect of skin care item is more preferably.
Preferably, the mass ratio of the Japanese Stephania Root stem cell extract and Honegsukle flower P.E is 0.35~1.4：5.More
Preferably, the mass ratio of the Japanese Stephania Root stem cell extract and Honegsukle flower P.E is 0.6：5.Present inventor passes through
Test of many times is found, when the mass ratio of Japanese Stephania Root stem cell extract and Honegsukle flower P.E is 0.35~1.4 in skin care item：5
When, the fungistatic effect of skin care item is preferable；When the mass ratio of Japanese Stephania Root stem cell extract and Honegsukle flower P.E is in skin care item
0.6：When 5, the fungistatic effect of skin care item is more preferably.
Preferably, the composition contains the component of following parts by weight：0.3 part of Japanese Stephania Root stem cell extract, Cortex Phellodendri carry
Take 4.1 parts of 3 parts of thing, 7.1 parts of Bitter Orang P.E, 2.5 parts of Honegsukle flower P.E and Rhizoma Zingiberis zerumbet extract.Present inventor
Find through test of many times, extracted when containing 0.3 part of Japanese Stephania Root stem cell extract, 3 parts of phellodendron extract, the dried immature fruit of citron orange in skin care item
When 4.1 parts of 7.1 parts of thing, 2.5 parts of Honegsukle flower P.E and Rhizoma Zingiberis zerumbet extract, the antiallergic effect and fungistatic effect of skin care item
It is all good.
As the second aspect of the invention, the present invention also provides a kind of skin care item, the skin care item include above-mentioned group
Compound.
Preferably, Japanese Stephania Root stem cell extract, phellodendron extract, Bitter Orang P.E, honeysuckle carry in the skin care item
It is 8.3~9.8% to take the quality percentage total amount of thing and Rhizoma Zingiberis zerumbet extract.Present inventor has found through test of many times, when
Japanese Stephania Root stem cell extract, phellodendron extract, Bitter Orang P.E, Honegsukle flower P.E and Rhizoma Zingiberis zerumbet extract in skin care item
Quality percentage total amount be 8.3~9.8% when, the antiallergic effect of skin care item is preferable.
Preferably, the Japanese Stephania Root stem cell extract is prepared by the following：
S1, Japanese Stephania Root new life root or branch sterilization, after removing xylem and marrow, be inoculated in inducing culture, induction training
Support and obtain cambial cell；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Japanese Stephania Root stem cell；
S4, gained Japanese Stephania Root stem cell obtain the Japanese Stephania Root stem cell extract through the micro-nano method broken wall of low temperature.
Wherein, the micro-nano method broken wall of low temperature refers to make its freezing in a low temperature of Japanese Stephania Root stem cell is placed on -170 DEG C, with
Melt at normal temperatures afterwards, so repeatedly so that the cell membrane of Japanese Stephania Root stem cell crushes；This method can be by the life of stem cell
Activity is assigned in skin care item with power of regeneration, is absorbed via skin, can be remarkably promoted nutrition and be penetrated into and absorb, can effectively be lifted
Skin initiative, improves skin quality from cell aspect, avoids the generation of phenomena of cutaneous irritation.
Preferably, the phellodendron extract is prepared by the following：Cortex Phellodendri bark is adopted after high speed bead mill method broken wall
Cortex Phellodendri bark extract is obtained with water extraction；
The Bitter Orang P.E is prepared by the following：Dried immature fruit of citron orange fruit is taken to obtain trifoliate orange using alcohol extracting method after ultrasonication
Real extract；
The Honegsukle flower P.E is prepared by the following：Extracting honeysuckle is after the micro-nano method broken wall of low temperature, using alcohol
Formulation obtains Honegsukle flower P.E；
The Rhizoma Zingiberis zerumbet extract is prepared by the following：Rhizoma Zingiberis zerumbet is taken after the micro-nano method broken wall of low temperature, using water
Formulation obtains Rhizoma Zingiberis zerumbet extract.Wherein, alcohol extracting method is preferably extracted using ethanol or methanol.
Relative to the prior art, beneficial effects of the present invention are：
The skin care item of the present invention contain Japanese Stephania Root stem cell extract, phellodendron extract, Bitter Orang P.E, honeysuckle and carry
Thing and Rhizoma Zingiberis zerumbet extract are taken, there is good antiallergic effect；In addition, the skin care item of the present invention are free of preservative, to skin
It is safe and non-toxic.
Embodiment
To better illustrate the object, technical solutions and advantages of the present invention, below in conjunction with specific embodiment to the present invention
It is described further.
Embodiment 1
A kind of embodiment of antiallergic composition in the present invention, the component containing following parts by weight：Done containing Japanese Stephania Root
0.07 part of cell extract, 6.8 parts of phellodendron extract, 0.9 part of Bitter Orang P.E, 9.5 parts of Honegsukle flower P.E and Rhizoma Zingiberis zerumbet carry
Take 1.1 parts of thing.
A kind of embodiment of anti-allergic skin care product in the present invention, the skin care item include above-mentioned composition, sad glycerine three
107 parts of 39 parts of ester, 21 parts of butanediol, 44 parts of Arabic gum and water, wherein, Japanese Stephania Root stem cell extract, phellodendron extract,
The quality percentage total amount of Bitter Orang P.E, Honegsukle flower P.E and Rhizoma Zingiberis zerumbet extract is 9%.
The preparation method of above-mentioned anti-allergic skin care product includes the following steps：
(1) Japanese Stephania Root stem cell extract is prepared by the following：
S1, the sterilization of Japanese Stephania Root new life branch, after removing xylem and marrow, be inoculated in inducing culture, Fiber differentiation obtains
Cambial cell；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Japanese Stephania Root stem cell；
S4, gained Japanese Stephania Root stem cell obtain the Japanese Stephania Root stem cell extract through the micro-nano method broken wall of low temperature；
(2) phellodendron extract is prepared by the following：Cortex Phellodendri bark is after high speed bead mill method broken wall, using water extraction
Obtain Cortex Phellodendri bark extract；
Bitter Orang P.E is prepared by the following：Dried immature fruit of citron orange fruit is taken to obtain trifoliate orange using ethanol extraction method after ultrasonication
Real extract；
Honegsukle flower P.E is prepared by the following：Extracting honeysuckle is carried after the micro-nano method broken wall of low temperature using methanol
Follow the example of to obtain Honegsukle flower P.E；
Rhizoma Zingiberis zerumbet extract is prepared by the following：Rhizoma Zingiberis zerumbet is taken after the micro-nano method broken wall of low temperature, using water extraction
Obtain Rhizoma Zingiberis zerumbet extract.
(3) by phellodendron extract, Bitter Orang P.E, Honegsukle flower P.E and Rhizoma Zingiberis zerumbet extract and Trivent OCG
107 parts of 39 parts, 21 parts of butanediol, 44 parts of Arabic gum and water mix, and after 38 degrees Celsius stir evenly, it is unnecessary to be filtered to remove
Moisture, obtains lotion, then adds Japanese Stephania Root stem cell extract and is uniformly dispersed up to a kind of antiallergy face cream.
Embodiment 2
A kind of embodiment of antiallergic composition in the present invention, the component containing following parts by weight：Done containing Japanese Stephania Root
1.1 parts of cell extract, 5.4 parts of phellodendron extract, 2.4 parts of Bitter Orang P.E, 7.2 parts of Honegsukle flower P.E and Rhizoma Zingiberis zerumbet carry
Take 2.5 parts of thing.
A kind of embodiment of anti-allergic skin care product in the present invention, the skin care item include above-mentioned composition, sad glycerine three
111 parts of 55 parts of ester, 23 parts of butanediol, 47 parts of Arabic gum and water, wherein, Japanese Stephania Root stem cell extract, phellodendron extract,
The quality percentage total amount of Bitter Orang P.E, Honegsukle flower P.E and Rhizoma Zingiberis zerumbet extract is 8.3%.
The preparation method of above-mentioned anti-allergic skin care product includes the following steps：
(1) Japanese Stephania Root stem cell extract is prepared by the following：
S1, the sterilization of Japanese Stephania Root new life root, after removing xylem and marrow, be inoculated in inducing culture, Fiber differentiation obtains
Cambial cell；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Japanese Stephania Root stem cell；
S4, gained Japanese Stephania Root stem cell obtain the Japanese Stephania Root stem cell extract through the micro-nano method broken wall of low temperature；
(2) phellodendron extract is prepared by the following：Cortex Phellodendri bark is after high speed bead mill method broken wall, using water extraction
Obtain Cortex Phellodendri bark extract；
Bitter Orang P.E is prepared by the following：Dried immature fruit of citron orange fruit is taken to obtain trifoliate orange using methanol extraction after ultrasonication
Real extract；
Honegsukle flower P.E is prepared by the following：Extracting honeysuckle is carried after the micro-nano method broken wall of low temperature using ethanol
Follow the example of to obtain Honegsukle flower P.E；
Rhizoma Zingiberis zerumbet extract is prepared by the following：Rhizoma Zingiberis zerumbet is taken after the micro-nano method broken wall of low temperature, using water extraction
Obtain Rhizoma Zingiberis zerumbet extract.
(3) by phellodendron extract, Bitter Orang P.E, Honegsukle flower P.E and Rhizoma Zingiberis zerumbet extract and Trivent OCG
111 parts of 55 parts, 23 parts of butanediol, 47 parts of Arabic gum and water mix, and after 37.2 degrees Celsius stir evenly, are filtered to remove more
Remaining moisture, obtains lotion, then adds Japanese Stephania Root stem cell extract and is uniformly dispersed up to a kind of antiallergy face cream.
Embodiment 3
A kind of embodiment of antiallergic composition in the present invention, the component containing following parts by weight：Done containing Japanese Stephania Root
0.3 part of cell extract, 3 parts of phellodendron extract, 7.1 parts of Bitter Orang P.E, 2.5 parts of Honegsukle flower P.E and Rhizoma Zingiberis zerumbet extraction
4.1 parts of thing.
A kind of embodiment of anti-allergic skin care product in the present invention, the skin care item include above-mentioned composition, sad glycerine three
89 parts of 33 parts of ester, 16 parts of butanediol, 37 parts of Arabic gum and water, wherein, Japanese Stephania Root stem cell extract, phellodendron extract, trifoliate orange
The quality percentage total amount of real extract, Honegsukle flower P.E and Rhizoma Zingiberis zerumbet extract is 9.8%.
The preparation method of above-mentioned anti-allergic skin care product includes the following steps：
(1) Japanese Stephania Root stem cell extract is prepared by the following：
S1, the sterilization of Japanese Stephania Root new life branch, after removing xylem and marrow, be inoculated in inducing culture, Fiber differentiation obtains
Cambial cell；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Japanese Stephania Root stem cell；
S4, gained Japanese Stephania Root stem cell obtain the Japanese Stephania Root stem cell extract through the micro-nano method broken wall of low temperature；
(2) phellodendron extract is prepared by the following：Cortex Phellodendri bark is after high speed bead mill method broken wall, using water extraction
Obtain Cortex Phellodendri bark extract；
Bitter Orang P.E is prepared by the following：Dried immature fruit of citron orange fruit is taken to obtain trifoliate orange using ethanol extraction method after ultrasonication
Real extract；
Honegsukle flower P.E is prepared by the following：Extracting honeysuckle is carried after the micro-nano method broken wall of low temperature using ethanol
Follow the example of to obtain Honegsukle flower P.E；
Rhizoma Zingiberis zerumbet extract is prepared by the following：Rhizoma Zingiberis zerumbet is taken after the micro-nano method broken wall of low temperature, using water extraction
Obtain Rhizoma Zingiberis zerumbet extract.
(3) by phellodendron extract, Bitter Orang P.E, Honegsukle flower P.E and Rhizoma Zingiberis zerumbet extract and Trivent OCG
89 parts of 33 parts, 16 parts of butanediol, 37 parts of Arabic gum and water mix, and after 36.8 degrees Celsius stir evenly, it is unnecessary to be filtered to remove
Moisture, obtains lotion, then adds Japanese Stephania Root stem cell extract and is uniformly dispersed up to a kind of antiallergy face cream.
Embodiment 4
A kind of embodiment of antiallergic composition in the present invention, the component containing following parts by weight：Done containing Japanese Stephania Root
2.2 parts of cell extract, 1.3 parts of phellodendron extract, 5.6 parts of Bitter Orang P.E, 5.8 parts of Honegsukle flower P.E and Rhizoma Zingiberis zerumbet carry
Take 7.7 parts of thing.
A kind of embodiment of anti-allergic skin care product in the present invention, the skin care item include above-mentioned composition, sad glycerine three
122 parts of 47 parts of ester, 25 parts of butanediol, 53 parts of Arabic gum and water, wherein, Japanese Stephania Root stem cell extract, phellodendron extract,
The quality percentage total amount of Bitter Orang P.E, Honegsukle flower P.E and Rhizoma Zingiberis zerumbet extract is 9.6%.
The preparation method of above-mentioned anti-allergic skin care product includes the following steps：
(1) Japanese Stephania Root stem cell extract is prepared by the following：
S1, the sterilization of Japanese Stephania Root new life root, after removing xylem and marrow, be inoculated in inducing culture, Fiber differentiation obtains
Cambial cell；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Japanese Stephania Root stem cell；
S4, gained Japanese Stephania Root stem cell obtain the Japanese Stephania Root stem cell extract through the micro-nano method broken wall of low temperature；
(2) phellodendron extract is prepared by the following：Cortex Phellodendri bark is after high speed bead mill method broken wall, using water extraction
Obtain Cortex Phellodendri bark extract；
Bitter Orang P.E is prepared by the following：Dried immature fruit of citron orange fruit is taken to obtain trifoliate orange using methanol extraction after ultrasonication
Real extract；
Honegsukle flower P.E is prepared by the following：Extracting honeysuckle is carried after the micro-nano method broken wall of low temperature using methanol
Follow the example of to obtain Honegsukle flower P.E；
Rhizoma Zingiberis zerumbet extract is prepared by the following：Rhizoma Zingiberis zerumbet is taken after the micro-nano method broken wall of low temperature, using water extraction
Obtain Rhizoma Zingiberis zerumbet extract.
(3) by phellodendron extract, Bitter Orang P.E, Honegsukle flower P.E and Rhizoma Zingiberis zerumbet extract and Trivent OCG
122 parts of 47 parts, 25 parts of butanediol, 53 parts of Arabic gum and water mix, and after 37 degrees Celsius stir evenly, it is unnecessary to be filtered to remove
Moisture, obtains lotion, then adds Japanese Stephania Root stem cell extract and is uniformly dispersed up to a kind of antiallergy face cream.
Embodiment 5
A kind of embodiment of antiallergic composition in the present invention, the component containing following parts by weight：Done containing Japanese Stephania Root
3.5 parts of cell extract, 0.6 part of phellodendron extract, 8.3 parts of Bitter Orang P.E, 1.3 parts of Honegsukle flower P.E and Rhizoma Zingiberis zerumbet carry
Take 9.2 parts of thing.
A kind of embodiment of anti-allergic skin care product in the present invention, the skin care item include above-mentioned composition, sad glycerine three
102 parts of 48 parts of ester, 23 parts of butanediol, 45 parts of Arabic gum and water, wherein, Japanese Stephania Root stem cell extract, phellodendron extract,
The quality percentage total amount of Bitter Orang P.E, Honegsukle flower P.E and Rhizoma Zingiberis zerumbet extract is 10.5%.
The preparation method of above-mentioned anti-allergic skin care product includes the following steps：
(1) Japanese Stephania Root stem cell extract is prepared by the following：
S1, the sterilization of Japanese Stephania Root new life branch, after removing xylem and marrow, be inoculated in inducing culture, Fiber differentiation obtains
Cambial cell；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Japanese Stephania Root stem cell；
S4, gained Japanese Stephania Root stem cell obtain the Japanese Stephania Root stem cell extract through the micro-nano method broken wall of low temperature；
(2) phellodendron extract is prepared by the following：Cortex Phellodendri bark is after high speed bead mill method broken wall, using water extraction
Obtain Cortex Phellodendri bark extract；
Bitter Orang P.E is prepared by the following：Dried immature fruit of citron orange fruit is taken to obtain trifoliate orange using ethanol extraction method after ultrasonication
Real extract；
Honegsukle flower P.E is prepared by the following：Extracting honeysuckle is carried after the micro-nano method broken wall of low temperature using ethanol
Follow the example of to obtain Honegsukle flower P.E；
Rhizoma Zingiberis zerumbet extract is prepared by the following：Rhizoma Zingiberis zerumbet is taken after the micro-nano method broken wall of low temperature, using water extraction
Obtain Rhizoma Zingiberis zerumbet extract.
(3) by phellodendron extract, Bitter Orang P.E, Honegsukle flower P.E and Rhizoma Zingiberis zerumbet extract and Trivent OCG
102 parts of 48 parts, 23 parts of butanediol, 45 parts of Arabic gum and water mix, and after 36.5 degrees Celsius stir evenly, are filtered to remove more
Remaining moisture, obtains lotion, then adds Japanese Stephania Root stem cell extract and is uniformly dispersed up to a kind of antiallergy face cream.
The antiallergic effect test of the skin care item of the present invention of embodiment 6
Test object：The face cream of embodiment 1~5 and the face cream 1~4 as control, wherein, control group 1~4
Differ only in Japanese Stephania Root stem cell extract, phellodendron extract and the Rhizoma Zingiberis zerumbet of face cream and the face cream of embodiment 3 extract
The mass ratio of thing is different, and its preparation method is same as Example 3；The Japanese Stephania Root stem cell extraction of the face cream of control group 1~4
The mass ratio of thing, phellodendron extract and Rhizoma Zingiberis zerumbet extract is followed successively by： 0.15：3：6.9；0.7：3：5.6；1.3：3：3.5；
1.8：3：2.7；
Test method：It is some to randomly select 20~35 years old youth of both sexes, is divided into nine groups altogether, every group of 45 people, nine groups of personnel
Respectively using baring skin after the face cream of embodiment 1~5 and the face cream smearing ear of control group 1~4, count after five minutes
The number of existing allergic phenomena (allergic symptom, which shows as skin, redness is occurred, itch or peel).
Test result：It is as shown in table 1 below；
The antiallergic effect of face cream in 1 present invention of table
| Face cream | Allergy number | Face cream | Allergy number |
| Embodiment 1 | 7 | Control group 1 | 2 |
| Embodiment 2 | 3 | Control group 2 | 2 |
| Embodiment 3 | 0 | Control group 3 | 3 |
| Embodiment 4 | 4 | Control group 4 | 3 |
| Embodiment 5 | 5 |
Interpretation of result：As shown in Table 1, the face cream in the present invention all has certain antiallergic effect, wherein implementing
The antiallergic effect of the face cream of example 2 and 3 and the face cream of control group 1~4 is preferable；Also, the face cream of embodiment 3 is anti-
The ability of allergy is best, and wherein the mass ratio of Japanese Stephania Root stem cell extract, phellodendron extract and Rhizoma Zingiberis zerumbet extract is 0.3：
3：4.1.
The fungistatic effect test of the skin care item of the present invention of embodiment 7
Test object：The face cream of embodiment 1~5 and the face cream 1~4 as control, wherein, control group 1~4
The mass ratio for differing only in Japanese Stephania Root stem cell extract and Honegsukle flower P.E of face cream and the face cream of embodiment 3
Difference, its preparation method are same as Example 3；Japanese Stephania Root stem cell extract and honeysuckle in the face cream of control group 1~4
The mass ratio of extract is 0.35 successively：5；0.96：5；1.19：5； 1.4：5；
Test method：
Staphylococcus, streptococcus, bacterium acidi propionici and corynebacteria, and LB solid mediums (i.e. LB tablets) are chosen, will
Face cream is dissolved into the aqueous solution that mass percent is 3.3% with water, by staphylococcus, streptococcus, bacterium acidi propionici and rod-like stem
Bacterium is according to quantity 1：1：1：Added after 1 mixing in the aqueous solution of face cream and form mixed solution, the concentration of bacterium is in mixed solution
1.5×106It is a, drip 4 drop mixed solutions on LB tablets with dropper respectively, 50 it is small when after measure the diameter of bacterial plaque, and calculate
Bacterial plaque average diameter.
Test result：It is as shown in table 2 below；
The fungistatic effect of the face cream of the present invention of table 2
| Face cream | Bacterial plaque diameter | Face cream | Bacterial plaque diameter |
| Embodiment 1 | 0.77cm | Control group 1 | 0.22cm |
| Embodiment 2 | 0.17cm | Control group 2 | 0.19cm |
| Embodiment 3 | 0cm | Control group 3 | 0.26cm |
| Embodiment 4 | 0.41cm | Control group 4 | 0.31cm |
| Embodiment 5 | 0.52cm |
Interpretation of result：As shown in upper table 2, the fungistatic effect of the face cream of embodiment 2,3 and control group 1~4 is preferable, right
The Japanese Stephania Root stem cell extract and the mass ratio of Honegsukle flower P.E answered all fall within 0.35~1.4：In the range of 5；Moreover,
Bacteriostasis using the face cream of embodiment 3 is optimal, wherein, the matter of Japanese Stephania Root stem cell extract and Honegsukle flower P.E
Amount is than being 0.6：5.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than to the present invention
The limitation of protection domain, although being explained in detail with reference to preferred embodiment to the present invention, those of ordinary skill in the art should
Work as understanding, can be to technical scheme technical scheme is modified or replaced equivalently, without departing from the reality of technical solution of the present invention
Matter and scope.
Claims (10)
1. a kind of antiallergic composition, it is characterised in that the composition contains the component of following parts by weight：Done containing Japanese Stephania Root
0.07~3.5 part of cell extract, 0.6~6.8 part of phellodendron extract, 0.9~8.3 part of Bitter Orang P.E, Honegsukle flower P.E
1.3~9.5 parts and 1.1~9.2 parts of Rhizoma Zingiberis zerumbet extract.
2. composition according to claim 1, it is characterised in that the Japanese Stephania Root stem cell extract, phellodendron extract
Mass ratio with Rhizoma Zingiberis zerumbet extract is 0.15~1.8：3：2.7~6.9.
3. composition according to claim 2, it is characterised in that the Japanese Stephania Root stem cell extract, phellodendron extract
Mass ratio with Rhizoma Zingiberis zerumbet extract is 0.3：3：4.1.
4. composition according to claim 1, it is characterised in that the Japanese Stephania Root stem cell extract and honeysuckle extraction
The mass ratio of thing is 0.35~1.4：5.
5. composition according to claim 4, it is characterised in that the Japanese Stephania Root stem cell extract and honeysuckle extraction
The mass ratio of thing is 0.6：5.
6. composition according to claim 1, it is characterised in that the composition contains the component of following parts by weight：Thousand
Golden 0.3 part of rattan stem cell extract, 3 parts of phellodendron extract, 7.1 parts of Bitter Orang P.E, 2.5 parts of Honegsukle flower P.E and Rhizoma Zingiberis zerumbet
4.1 parts of extract.
7. a kind of anti-allergic skin care product, it is characterised in that the skin care item include such as claim 1~6 any one of them group
Compound.
8. skin care item according to claim 7, it is characterised in that Japanese Stephania Root stem cell extract, Huang in the skin care item
Cypress extract, Bitter Orang P.E, the quality percentage total amount of Honegsukle flower P.E and Rhizoma Zingiberis zerumbet extract are 8.3~9.8%.
9. skin care item according to claim 7, it is characterised in that the Japanese Stephania Root stem cell extract is by the following method
Obtain：
S1, Japanese Stephania Root new life root or branch sterilization, after removing xylem and marrow, be inoculated in inducing culture, Fiber differentiation obtains
Obtain cambial cell；
S2, the cambial cell access proliferated culture medium, shaking table culture obtains unicellular through squamous subculture；
S3, by it is described it is unicellular be inoculated in proliferated culture medium through expand cultivate, obtain Japanese Stephania Root stem cell；
S4, gained Japanese Stephania Root stem cell obtain the Japanese Stephania Root stem cell extract through the micro-nano method broken wall of low temperature.
10. skin care item according to claim 7, it is characterised in that the phellodendron extract is prepared by the following：It is yellow
Cypress skin obtains Cortex Phellodendri bark extract after high speed bead mill method broken wall using water extraction；
The Bitter Orang P.E is prepared by the following：Dried immature fruit of citron orange fruit is taken after ultrasonication, obtaining the dried immature fruit of citron orange using alcohol extracting method carries
Take thing；
The Honegsukle flower P.E is prepared by the following：Extracting honeysuckle is after the micro-nano method broken wall of low temperature, using alcohol extracting method
Obtain Honegsukle flower P.E；
The Rhizoma Zingiberis zerumbet extract is prepared by the following：Rhizoma Zingiberis zerumbet is taken after the micro-nano method broken wall of low temperature, using water extraction
Obtain Rhizoma Zingiberis zerumbet extract.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1352640A1 (en) * | 2000-12-15 | 2003-10-15 | Kabushiki Kaisha Yakult Honsha | Compositions for retarding skin aging |
| CN104920227A (en) * | 2015-07-15 | 2015-09-23 | 广东融和生态农业有限公司 | Stem cell tissue culture seedling raising technique |
| CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
-
2017
- 2017-12-04 CN CN201711272442.1A patent/CN108030757A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1352640A1 (en) * | 2000-12-15 | 2003-10-15 | Kabushiki Kaisha Yakult Honsha | Compositions for retarding skin aging |
| CN104920227A (en) * | 2015-07-15 | 2015-09-23 | 广东融和生态农业有限公司 | Stem cell tissue culture seedling raising technique |
| CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
Non-Patent Citations (1)
| Title |
|---|
| 王建新 等: "《化妆品植物原料大全》", 30 June 2012, 中国纺织出版社 * |
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