CN108030094A - A kind of processing method of loosestrife health-care paste - Google Patents

A kind of processing method of loosestrife health-care paste Download PDF

Info

Publication number
CN108030094A
CN108030094A CN201810029187.6A CN201810029187A CN108030094A CN 108030094 A CN108030094 A CN 108030094A CN 201810029187 A CN201810029187 A CN 201810029187A CN 108030094 A CN108030094 A CN 108030094A
Authority
CN
China
Prior art keywords
loosestrife
health
pumpkin
leaf
care paste
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201810029187.6A
Other languages
Chinese (zh)
Inventor
张传海
赵升云
杨斌
李宝银
林志銮
华伟平
储华
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wuyi University
Original Assignee
Wuyi University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wuyi University filed Critical Wuyi University
Priority to CN201810029187.6A priority Critical patent/CN108030094A/en
Publication of CN108030094A publication Critical patent/CN108030094A/en
Pending legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105Plant extracts, their artificial duplicates or their derivatives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/125Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17Amino acids, peptides or proteins
    • A23L33/18Peptides; Protein hydrolysates
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/42Cucurbitaceae (Cucumber family)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/46Eucommiaceae (Eucommia family), e.g. hardy rubber tree
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Polymers & Plastics (AREA)
  • Botany (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Nutrition Science (AREA)
  • Medical Informatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Public Health (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physiology (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

The invention belongs to technical field of food healthcare, disclose a kind of processing method of loosestrife health-care paste, it is raw materials used mainly to have loosestrife, sodium ascorbate solution, cellulase, pectase, He Ziguo, malic acid solution, folium cortex eucommiae, selenium aqueous solution, wherein sodium ascorbate solution concentration is 5%, the concentration of malic acid solution is 20%, and the concentration of selenium aqueous solution is 7%.The health-care paste that the present invention processes is using loosestrife that is green and healthy, full of nutrition, deriving from a wealth of sources and myrobalan fruit as primary raw material, not only reduce production cost, and enrich the new varieties of health-care paste and the nutritional ingredient of health-care paste, easy to carry, color stability, pure in mouth feel, improve economic benefit, has analgesia stomach invigorating, tonifying middle-Jiao and Qi, hepatic cholagogic, anti-oxidant and other effects.This method can improve the nutritive value and economic value of health-care paste, enrich the species of loosestrife product.

Description

A kind of processing method of loosestrife health-care paste
Technical field
The invention belongs to technical field of food healthcare, more particularly to a kind of processing method of loosestrife health-care paste.
Background technology
At present, the prior art commonly used in the trade is such:
Loosestrife (Lysimachia clethroides), is Primulaceae Lysimachia herbaceos perennial, high by 40 ~100cm;It can be eaten as vegetables, it is nontoxic.Small because spending, white is as gone here and there string of beads pearl, so loosestrife of gaining the name.Contain in cauline leaf Abundant mineral matter, especially with the content highest of potassium, and contains flavonoids etc..Loosestrife has clearing heat and promoting diuresis, Huoxue San " The effect of the stasis of blood, removing toxicity for eliminating carbuncles.The medical value of loosestrife is high, but deep processed product is less, using loosestrife as raw material, is processed into Health-care paste, in the market is there is not yet Related product lists.The nutriment of existing loosestrife cannot be by efficient absorption and by pearl The more single deficiency of product category that dish processes.
Pumpkin (Cucurbita moschata Duch) also known as wheat melon, pumpkin, golden wax gourd etc., belong to Curcurbitaceae Cucurbita one Year raw herbaceous plant, is one of traditional vegetables in China, and pumpkin is the important industrial crops in China, and annual output is up to 4,100,000 tons, position Rank first in the world.The stock number of pumpkin stem end leaf flower is very huge, both at home and abroad the nutritional ingredient to pumpkin fruit and health-care effect into Go substantial amounts of research and report, but the research report to pumpkin cauline leaf flower is seldom at present, and pumpkin cauline leaf flower is except minimal amount Outside being eaten, most of resource is dropped.
The nutrition and health care and utilization of cushaw leaf are still in infancy both at home and abroad.Such as to cushaw leaf flavones, polyphenol Extraction and activity with protein have a small amount of report.Dried pumpkin leaf protein content is up to more than 30%, and amino acid classes are complete, battalion It is high to support value.But since cushaw leaf fiber content is high, general buck heating recovery rate is very low.Polysaccharide contains in cushaw leaf Amount is higher, but so far there are no studies report, prepares cushaw leaf polypeptide using pumpkin leaf protein and also has no research.
The reducing blood lipid of polysaccharide energy, hypoglycemic, antiulcer, anti-cancer and cancer-preventing, removing free radical etc..It is manufactured that using polysaccharide Diabetes, the new drug of AIDS virus resisting are treated, there is the effect of notable;It has also been found that, the sugar chain of polysaccharide is thin in animal in recent years There is decisive role in terms of born of the same parents' division, differentiation, growth and anti-aging, polysaccharide, which is already shown, is more widely applied prospect.It is more Peptide is to be extracted directly from plant or hydrolyze and obtain through protein, it is highly important bioactive ingredients, it absorbs machine System is better than amino acid, and with the incomparable physiological function of amino acid.The infiltration of biologically active peptide specific ionization amino acid is forced down, Absorptivity is high, will not cause the adverse reactions such as diarrhea after food;Biologically active peptide has compared with low antigenicity again, and allergy will not be caused anti- Should.Additionally due to the diversity and complexity of biologically active peptide structure determine that it has work in cell physiological and adjusting metabolic function Property effect, substantial amounts of research confirm these polypeptides also have potential lowering blood pressure and blood fat, it is anti-oxidant, antiviral, antibacterial, Promote growth, calm, reduction cholesterol, promote mineral absorption, improve the functions such as metabolism, enhancing body immunity, These functions are crude protein and it is formed not available for single amino acid.
At present, the technique that is typically prepared of polysaccharide is extraction-de- albumen-concentration-drying.The extracting method of polysaccharide is to pass Based on solvent heating extraction of uniting, in addition, ultrasonic wave assisted extraction, microwave radiation exaraction, zymohydrolysis extracting method are also increasingly people Pay attention to.De- protein method need to be used a large amount of toxic solvent chloroforms and n-butanol, protein is broken based on traditional Sevage methods It is bad.In recent years using the method for various proteolytic enzyme protolysates gradually into trend.In extraction, de- albumen, concentration, dry each link In, the use of high temperature and organic solvent causes that active ingredient is easily destroyed, it is high to consume energy.Due to containing macromolecule component, often There is secondary phenomenon not soluble in water, hamper human body and the effective of polysaccharide is absorbed.
In this context, new enzyme and membrane technology are introduced, improves the efficiency of enzymolysis and extraction, avoid high temperature, avoid it is organic molten A large amount of uses of agent, are the active demands in the direction and market of technology development.
In conclusion problem existing in the prior art is:
The nutriment of existing loosestrife cannot be more single by efficient absorption and the product category processed by loosestrife Deficiency;
The alimentary health-care function for being not bound with cushaw leaf is developed;
The prior art does not avoid being heated at high temperature the destruction to active ingredient;
The material extracted has phenomenon not soluble in water.
Solve the difficulty and meaning of above-mentioned technical problem:
The present invention can improve the nutritive value and economic value and the species for enriching loosestrife product of health-care paste.
Gained pumpkin leaf polyose and polypeptide of the invention belong to natural health-care products preparation, safe and non-toxic, suitable for medicine and protect The industries such as strong product, food antioxidant, feed addictive, realize the comprehensive utilization of cushaw leaf resource.
The content of the invention
In view of the problems of the existing technology, the present invention provides a kind of processing method of loosestrife health-care paste.
The present invention is achieved in that a kind of processing method of loosestrife health-care paste, the processing of the loosestrife health-care paste Method includes following steps:
Loosestrife processing:The loosestrife of no disease and pests harm is chosen, cleans up and the anti-of 15kg is added in backward 10kg loosestrives Bad hematic acid sodium solution is beaten, and pearl puree is made, added into 10kg pearl purees 0.008kg cellulase, The pectase of 0.005kg, 1.5h is digested in the environment of 50 DEG C, and pearl vegetable juice is made, spare;
Myrobalan fruit is handled:The malic acid solution of 10kg, the leaching of myrobalan fruit are added into the 10kg myrobalan fruit after fresh, cleaning It is beaten after bubble 3h, myrobalan's pulp is made;
It is prepared by extracting solution:Win to be placed in the selenium aqueous solution of 10kg after the fresh folium cortex eucommiae clear water of 10kg elutes and extracted, extracted It is 60 DEG C, time control 30min to take temperature control, repeats extraction 5 times, extract is mixed, is concentrated in the environment of 60 DEG C To the 24% of original volume, bark of eucommia leaf extract and folium cortex eucommiae filter residue is made;
Extraction of the pumpkin into liquid separation:By cushaw leaf powder, with 1:7~1:17 water mixing, through historrhexis's machine homogenate 1~ After 10min, then with solid-liquid ratio 1:30~1:70,200~800W of ultrasonic power, 3000~5000U/ of enzyme concentration cellulose enzyme amount G pumpkin leaf dried powders, xylosidase 500~1500U/g pumpkin leaf dried powders, pH value 5.5~7.0, digest under the conditions of 40~65 DEG C and carry Take 1~2h;Wherein, cellulose enzyme amount is 3~12 than xylose enzyme amount multiple;Obtained mixed liquor will be extracted through two kinds of different retentions The ultrafiltration membrance filter removal of impurities decoloration of molecular weight, retaining molecular weight 18000~27000 used in first time, obtains pumpkin leaf polyose and carries Take liquid;Second of retaining molecular weight 7000~8000 used, second of ultra-filter retentate is as the original for preparing cushaw leaf polypeptide Material, enzyme concentration 80~280U/g of papain, its pumpkin leaf dried powder enzyme activity 30U/mg, pepsin 50~150U/g cushaw leafs More than dry powder enzyme activity 120U/mg, pH value 5.5~7.0,3~7h of enzymolysis and extraction, obtains protein enzymatic hydrolyzate under the conditions of 40~65 DEG C; Wherein, Papain enzyme amount is 2.0~7.0 than stomach cardia enzyme amount multiple;By polysaccharide extraction liquid and protein enzymatic hydrolyzate, pass through respectively NF membrane is concentrated, and wherein the molecular cut off of film is 200~300,0.20~0.25Mpa of outlet pressure, concentrated solution density For 1.15~1.20g/mL;Concentrate obtains pumpkin leaf polyose and cushaw leaf polypeptide, vacuum freeze drying through vacuum freeze drying again Condition is that liquid is cooled to -25~-45 DEG C, and hothouse vacuum is 0.088~0.12MPa, 8~12mm of material thickness, heating plate 25~40 DEG C of temperature, condenser temperature -35~-62 DEG C, after freeze-drying, obtain pumpkin into liquid separation;
Mixing:Pearl vegetable juice 5kg, myrobalan's pulp 2kg, folium cortex eucommiae filter residue 2kg, pumpkin is taken to be uniformly mixed into liquid separation 1.5kg, Mixed liquor is made.
Further, after blend step, also need to carry out:
Homogeneous:By mixed liquor temperature be 50 DEG C, pressure be 30Mpa under conditions of carry out homogeneous, homogeneous 5 times;
Lyophilization:By the mixed liquor after homogeneous under the conditions of -20 DEG C, 6h is freezed, then presses 12kg/m in useful load2、 It is dry under conditions of operating pressure 80Pa, parsing pressure 20Pa, 40 DEG C of temperature, mixed material is made;
Crush:Dried mixed material is crushed, particle diameter is 500 μm, and mixed material powder is made;
It is prepared by health-care paste:The extracting solution of 3kg is added into 10kg mixed material powder, is deployed into paste, pearl is made in sizing Dish health-care paste.
Further, the sodium ascorbate solution concentration is 5%.
Further, the concentration of the malic acid solution is 20%.
Further, the concentration of the selenium aqueous solution is 7%.
Further, pumpkin is into liquid separation, pumpkin leaf polyose:Total sugar content >=88%, moisture≤5.0%;Cushaw leaf polypeptide: Content of peptides >=75%, moisture≤5.0%.
Further, pumpkin is into needing to carry out before the extraction of liquid separation:It will pick fresh to October after annual pumpkin result Processing is dried after impurity elimination is handled in cushaw leaf;Dry cushaw leaf is carried out after suitably crushing with machinery, is placed in drying Shady place is spare.
Another object of the present invention is to provide a kind of loosestrife health-care paste.
Advantages of the present invention and good effect are:The processing method of the loosestrife health-care paste, with it is green and healthy, nutrition is rich Richness, the loosestrife derived from a wealth of sources and myrobalan fruit are primary raw material, not only reduce production cost, and enrich the new of health-care paste The nutritional ingredient of kind and health-care paste, easy to carry, color stability, pure in mouth feel, improve economic benefit, and there is analgesia to be good for Stomach, tonifying middle-Jiao and Qi, hepatic cholagogic, anti-oxidant and other effects.
This method can improve the nutritive value and economic value and the species for enriching loosestrife product of health-care paste.
The present invention utilizes cushaw leaf high efficiency extraction pumpkin leaf polyose and polypeptide, introduces enzymolysis and membrane technology is extracted, divided From, concentration, decoloration etc. process, product quality and yield can be improved, required time is short, and extraction process temperature is low, avoids high temperature The destruction to active ingredient is heated, in addition, the pumpkin leaf polyose and polypeptide that are extracted by the present invention, without secondary not soluble in water Phenomenon.
Gained pumpkin leaf polyose and polypeptide of the invention belong to natural health-care products preparation, safe and non-toxic, suitable for medicine and protect The industries such as strong product, food antioxidant, feed addictive, realize the comprehensive utilization of cushaw leaf resource.
Brief description of the drawings
Fig. 1 is the processing method flow chart of pearl dish health-care paste provided in an embodiment of the present invention;
Fig. 2 is extraction flow chart of the pumpkin provided in an embodiment of the present invention into liquid separation.
Embodiment
In order to further understand the content, features and effects of the present invention, the following examples are hereby given, and coordinate attached drawing Describe in detail as follows.
The processing method of loosestrife health-care paste provided in an embodiment of the present invention, the processing method institute of the loosestrife health-care paste Mainly there are loosestrife, sodium ascorbate solution, cellulase, pectase, He Ziguo, malic acid solution, folium cortex eucommiae, selenium with raw material Aqueous solution.
Fig. 1, the processing method of loosestrife health-care paste provided in an embodiment of the present invention have following steps:
S101, loosestrife processing:The loosestrife of no disease and pests harm is chosen, cleans up in backward 10kg loosestrives and adds 15kg Sodium ascorbate solution be beaten, pearl puree is made, added into 10kg pearl purees 0.008kg cellulase, The pectase of 0.005kg, 1.5h is digested in the environment of 50 DEG C, and pearl vegetable juice is made, spare;
S102, the processing of myrobalan fruit:The malic acid solution of 10kg is added into the 10kg myrobalan fruit after fresh, cleaning, is scolded It is beaten after sub- fruit immersion 3h, myrobalan's pulp is made;
It is prepared by S103, extracting solution:Win to be placed in the selenium aqueous solution of 10kg after the fresh folium cortex eucommiae clear water of 10kg elutes and extracted Take, extraction temperature control is 60 DEG C, time control 30min, repeats extraction 5 times, extract is mixed, in the environment of 60 DEG C The 24% of original volume is concentrated into, bark of eucommia leaf extract and folium cortex eucommiae filter residue is made;
S104, extraction of the pumpkin into liquid separation;
S105, mixing:Pearl vegetable juice 5kg, myrobalan's pulp 2kg, folium cortex eucommiae filter residue 2kg, pumpkin is taken to be mixed into liquid separation 1.5kg Uniformly, mixed liquor is made;
S106, homogeneous:By mixed liquor temperature be 50 DEG C, pressure be 30Mpa under conditions of carry out homogeneous, homogeneous 5 times;
S107, lyophilization:By the mixed liquor after homogeneous under the conditions of -20 DEG C, 6h is freezed, is then pressed in useful load 12kg/m2, it is operating pressure 80Pa, parsing pressure 20Pa, dry under conditions of 40 DEG C of temperature, mixed material is made;
S108, crushes:Dried mixed material is crushed, particle diameter is 500 μm, and mixed material powder is made;
It is prepared by S109, health-care paste:The extracting solution of 3kg is added into 10kg mixed material powder, is deployed into paste, sizing is made Loosestrife health-care paste.
As shown in Fig. 2, the extracting method of the pumpkin into liquid separation includes:
S201:By cushaw leaf powder, with 1:7~1:17 water mixing, after historrhexis's machine is homogenized 1~10min, then with material Liquor ratio 1:30~1:70,200~800W of ultrasonic power, enzyme concentration cellulose enzyme amount 3000~5000U/g pumpkin leaf dried powder (enzymes More than 200U/mg living), xylosidase 500~1500U/g pumpkins leaf dried powder (more than enzyme activity 60U/mg), pH value 5.5~7.0,40 1~2h of enzymolysis and extraction under the conditions of~65 DEG C;Wherein, cellulose enzyme amount is 3~12 than xylose enzyme amount multiple;
S202:Obtained ultrafiltration membrance filter removal of impurities of the mixed liquor through two kinds of different molecular weight cut off will be extracted to decolourize, first Secondary retaining molecular weight 18000~27000 used, obtains cushaw leaf polysaccharide extraction liquid;Second of retaining molecular weight 7000 used ~8000, second of ultra-filter retentate is as the raw material for preparing cushaw leaf polypeptide, enzyme concentration 80~280U/g of papain, its Pumpkin leaf dried powder enzyme activity 30U/mg, more than pepsin 50~150U/g pumpkin leaf dried powder enzyme activity 120U/mg, pH value 5.5~ 7.0,3~7h of enzymolysis and extraction, obtains protein enzymatic hydrolyzate under the conditions of 40~65 DEG C;Wherein, Papain enzyme amount is than stomach cardia enzyme amount Multiple is 2.0~7.0;
S203:By polysaccharide extraction liquid and protein enzymatic hydrolyzate, concentrated respectively by NF membrane, wherein the retention molecule of film Measure as 200~300,0.2~0.25Mpa of outlet pressure, concentrated solution density is 1.15~1.20g/mL;Concentrate is cold through vacuum again Lyophilized dry pumpkin leaf polyose and cushaw leaf polypeptide, vacuum freeze drying condition is that liquid is cooled to -25~-45 DEG C, hothouse Vacuum is 0.088~0.12MPa, and 8~12mm of material thickness, 25~40 DEG C of temperature of heating plate, condenser temperature -35~-62 DEG C, pass through After crossing freeze-drying, pumpkin is obtained into liquid separation.
The sodium ascorbate solution concentration is 5%, and the concentration of the malic acid solution is 20%, the selenium aqueous solution Concentration is 7%.
Pumpkin is into liquid separation, pumpkin leaf polyose:Total sugar content >=88%, moisture≤5.0%;Cushaw leaf polypeptide:Polypeptide contains Amount >=75%, moisture≤5.0%.
Pumpkin is into needing to carry out before the extraction of liquid separation:The fresh pumpkin leaf picked to October after annual pumpkin result is passed through After living together reason in the past, processing is dried;Dry cushaw leaf is carried out after suitably crushing with machinery, it is standby to be placed in dry shady place With.
With reference to specific embodiment, the invention will be further described.
Embodiment 1
The processing method of loosestrife health-care paste provided in an embodiment of the present invention, the processing method bag of the loosestrife health-care paste Include following steps:
Loosestrife processing:The loosestrife of no disease and pests harm is chosen, cleans up and the anti-of 15kg is added in backward 10kg loosestrives Bad hematic acid sodium solution is beaten, and pearl puree is made, added into 10kg pearl purees 0.008kg cellulase, The pectase of 0.005kg, 1.5h is digested in the environment of 50 DEG C, and pearl vegetable juice is made, spare;
Myrobalan fruit is handled:The malic acid solution of 10kg, the leaching of myrobalan fruit are added into the 10kg myrobalan fruit after fresh, cleaning It is beaten after bubble 3h, myrobalan's pulp is made;
It is prepared by extracting solution:Win to be placed in the selenium aqueous solution of 10kg after the fresh folium cortex eucommiae clear water of 10kg elutes and extracted, extracted It is 60 DEG C, time control 30min to take temperature control, repeats extraction 5 times, extract is mixed, is concentrated in the environment of 60 DEG C To the 24% of original volume, bark of eucommia leaf extract and folium cortex eucommiae filter residue is made;
Extraction of the pumpkin into liquid separation:By cushaw leaf powder, with 1:7 water mixing, after historrhexis's machine is homogenized 1~10min, Again with solid-liquid ratio 1:30,200~800W of ultrasonic power, enzyme concentration cellulose enzyme amount 3000~5000U/g pumpkin leaf dried powder (enzymes More than 200U/mg living), xylosidase 500~1500U/g pumpkins leaf dried powder (more than enzyme activity 60U/mg), pH value 5.5~7.0,40 1~2h of enzymolysis and extraction under the conditions of~65 DEG C;Wherein, cellulose enzyme amount is 3~12 than xylose enzyme amount multiple;To extract obtain it is mixed Close ultrafiltration membrance filter of the liquid through two kinds of different molecular weight cut off and clean and decolourize, retaining molecular weight 18000 used in first time~ 27000, obtain cushaw leaf polysaccharide extraction liquid;Second of retaining molecular weight 7000~8000 used, second of ultra-filter retentate are made To prepare the raw material of cushaw leaf polypeptide, enzyme concentration 80~280U/g of papain, its pumpkin leaf dried powder enzyme activity 30U/mg, stomach egg White more than enzyme 50~150U/g pumpkin leaf dried powder enzyme activity 120U/mg, pH value 5.5~7.0, the enzymolysis and extraction under the conditions of 40~65 DEG C 3~7h, obtains protein enzymatic hydrolyzate;Wherein, Papain enzyme amount is 2.0~7 than stomach cardia enzyme amount multiple;By polysaccharide extraction liquid and egg White enzymolysis liquid, is concentrated by NF membrane respectively, and wherein the molecular cut off of film is 200~300, and outlet pressure 0.20~ 0.25Mpa, concentrated solution density are 1.15~1.20g/mL;Concentrate obtains pumpkin leaf polyose and cushaw leaf through vacuum freeze drying again Polypeptide, vacuum freeze drying condition are that liquid is cooled to -25~-45 DEG C, and hothouse vacuum is 0.088~0.12MPa, and material is thick 8~12mm is spent, 25~40 DEG C of temperature of heating plate, condenser temperature -35~-62 DEG C, after freeze-drying, obtain pumpkin component Liquid;
Mixing:Pearl vegetable juice 5kg, myrobalan's pulp 2kg, folium cortex eucommiae filter residue 2kg, pumpkin is taken to be uniformly mixed into liquid separation 1.5kg, Mixed liquor is made.
After blend step, also need to carry out:
Homogeneous:By mixed liquor temperature be 50 DEG C, pressure be 30Mpa under conditions of carry out homogeneous, homogeneous 5 times;
Lyophilization:By the mixed liquor after homogeneous under the conditions of -20 DEG C, 6h is freezed, then presses 12kg/m in useful load2、 It is dry under conditions of operating pressure 80Pa, parsing pressure 20Pa, 40 DEG C of temperature, mixed material is made;
Crush:Dried mixed material is crushed, particle diameter is 500 μm, and mixed material powder is made;
It is prepared by health-care paste:The extracting solution of 3kg is added into 10kg mixed material powder, is deployed into paste, pearl is made in sizing Dish health-care paste.
Embodiment 2
The processing method of loosestrife health-care paste provided in an embodiment of the present invention, the processing method bag of the loosestrife health-care paste Include following steps:
Loosestrife processing:The loosestrife of no disease and pests harm is chosen, cleans up and the anti-of 15kg is added in backward 10kg loosestrives Bad hematic acid sodium solution is beaten, and pearl puree is made, added into 10kg pearl purees 0.008kg cellulase, The pectase of 0.005kg, 1.5h is digested in the environment of 50 DEG C, and pearl vegetable juice is made, spare;
Myrobalan fruit is handled:The malic acid solution of 10kg, the leaching of myrobalan fruit are added into the 10kg myrobalan fruit after fresh, cleaning It is beaten after bubble 3h, myrobalan's pulp is made;
It is prepared by extracting solution:Win to be placed in the selenium aqueous solution of 10kg after the fresh folium cortex eucommiae clear water of 10kg elutes and extracted, extracted It is 60 DEG C, time control 30min to take temperature control, repeats extraction 5 times, extract is mixed, is concentrated in the environment of 60 DEG C To the 24% of original volume, bark of eucommia leaf extract and folium cortex eucommiae filter residue is made;
Extraction of the pumpkin into liquid separation:By cushaw leaf powder, with 1:17 water mixing, 1~10min is homogenized through historrhexis's machine Afterwards, then with solid-liquid ratio 1:70,200~800W of ultrasonic power, enzyme concentration cellulose enzyme amount 3000~5000U/g pumpkin leaf dried powders (more than enzyme activity 200U/mg), xylosidase 500~1500U/g pumpkins leaf dried powder (more than enzyme activity 60U/mg), pH value 5.5~7.0, 1~2h of enzymolysis and extraction under the conditions of 40~65 DEG C;Wherein, cellulose enzyme amount is 3~12 than xylose enzyme amount multiple;Extraction is obtained Ultrafiltration membrance filter of the mixed liquor through two kinds of different molecular weight cut off clean decoloration, retaining molecular weight 18000 used in first time ~27000, obtain cushaw leaf polysaccharide extraction liquid;Second of retaining molecular weight 7000~8000 used, second of ultra-filter retentate As the raw material for preparing cushaw leaf polypeptide, enzyme concentration 80~280U/g of papain, its pumpkin leaf dried powder enzyme activity 30U/mg, stomach More than protease 50~150U/g pumpkin leaf dried powder enzyme activity 120U/mg, pH value 5.5~7.0, digests under the conditions of 40~65 DEG C and carries 3~7h is taken, obtains protein enzymatic hydrolyzate;Wherein, Papain enzyme amount is 2.0~7 than stomach cardia enzyme amount multiple;By polysaccharide extraction liquid and Protein enzymatic hydrolyzate, is concentrated by NF membrane respectively, and wherein the molecular cut off of film is 200~300, and outlet pressure 0.20~ 0.25Mpa, concentrated solution density are 1.15~1.20g/mL;Concentrate obtains pumpkin leaf polyose and cushaw leaf through vacuum freeze drying again Polypeptide, vacuum freeze drying condition are that liquid is cooled to -25~-45 DEG C, and hothouse vacuum is 0.088~0.12MPa, and material is thick 8~12mm is spent, 25~40 DEG C of temperature of heating plate, condenser temperature -35~-62 DEG C, after freeze-drying, obtain pumpkin component Liquid;
Mixing:Pearl vegetable juice 5kg, myrobalan's pulp 2kg, folium cortex eucommiae filter residue 2kg, pumpkin is taken to be uniformly mixed into liquid separation 1.5kg, Mixed liquor is made.
After blend step, also need to carry out:
Homogeneous:By mixed liquor temperature be 50 DEG C, pressure be 30Mpa under conditions of carry out homogeneous, homogeneous 5 times;
Lyophilization:By the mixed liquor after homogeneous under the conditions of -20 DEG C, 6h is freezed, then presses 12kg/m in useful load2、 It is dry under conditions of operating pressure 80Pa, parsing pressure 20Pa, 40 DEG C of temperature, mixed material is made;
Crush:Dried mixed material is crushed, particle diameter is 500 μm, and mixed material powder is made;
It is prepared by health-care paste:The extracting solution of 3kg is added into 10kg mixed material powder, is deployed into paste, pearl is made in sizing Dish health-care paste.
Embodiment 3
The processing method of loosestrife health-care paste provided in an embodiment of the present invention, the processing method bag of the loosestrife health-care paste Include following steps:
Loosestrife processing:The loosestrife of no disease and pests harm is chosen, cleans up and the anti-of 15kg is added in backward 10kg loosestrives Bad hematic acid sodium solution is beaten, and pearl puree is made, added into 10kg pearl purees 0.008kg cellulase, The pectase of 0.005kg, 1.5h is digested in the environment of 50 DEG C, and pearl vegetable juice is made, spare;
Myrobalan fruit is handled:The malic acid solution of 10kg, the leaching of myrobalan fruit are added into the 10kg myrobalan fruit after fresh, cleaning It is beaten after bubble 3h, myrobalan's pulp is made;
It is prepared by extracting solution:Win to be placed in the selenium aqueous solution of 10kg after the fresh folium cortex eucommiae clear water of 10kg elutes and extracted, extracted It is 60 DEG C, time control 30min to take temperature control, repeats extraction 5 times, extract is mixed, is concentrated in the environment of 60 DEG C To the 24% of original volume, bark of eucommia leaf extract and folium cortex eucommiae filter residue is made;
Extraction of the pumpkin into liquid separation:By cushaw leaf powder, with 1:12 water mixing, 1~10min is homogenized through historrhexis's machine Afterwards, then with solid-liquid ratio 1:50,200~800W of ultrasonic power, enzyme concentration cellulose enzyme amount 3000~5000U/g pumpkin leaf dried powders (more than enzyme activity 200U/mg), xylosidase 500~1500U/g pumpkins leaf dried powder (more than enzyme activity 60U/mg), pH value 5.5~7.0, 1~2h of enzymolysis and extraction under the conditions of 40~65 DEG C;Wherein, cellulose enzyme amount is 3~12 than xylose enzyme amount multiple;Extraction is obtained Ultrafiltration membrance filter of the mixed liquor through two kinds of different molecular weight cut off clean decoloration, retaining molecular weight 18000 used in first time ~27000, obtain cushaw leaf polysaccharide extraction liquid;Second of retaining molecular weight 7000~8000 used, second of ultra-filter retentate As the raw material for preparing cushaw leaf polypeptide, enzyme concentration 80~280U/g of papain, its pumpkin leaf dried powder enzyme activity 30U/mg, stomach More than protease 50~150U/g pumpkin leaf dried powder enzyme activity 120U/mg, pH value 5.5~7.0, digests under the conditions of 40~65 DEG C and carries 3~7h is taken, obtains protein enzymatic hydrolyzate;Wherein, Papain enzyme amount is 2.0~7 than stomach cardia enzyme amount multiple;By polysaccharide extraction liquid and Protein enzymatic hydrolyzate, is concentrated by NF membrane respectively, and wherein the molecular cut off of film is 200~300, and outlet pressure 0.20~ 0.25Mpa, concentrated solution density are 1.15~1.20g/mL;Concentrate obtains pumpkin leaf polyose and cushaw leaf through vacuum freeze drying again Polypeptide, vacuum freeze drying condition are that liquid is cooled to -25~-45 DEG C, and hothouse vacuum is 0.088~0.12MPa, and material is thick 8~12mm is spent, 25~40 DEG C of temperature of heating plate, condenser temperature -35~-62 DEG C, after freeze-drying, obtain pumpkin component Liquid;
Mixing:Pearl vegetable juice 5kg, myrobalan's pulp 2kg, folium cortex eucommiae filter residue 2kg, pumpkin is taken to be uniformly mixed into liquid separation 1.5kg, Mixed liquor is made.
After blend step, also need to carry out:
Homogeneous:By mixed liquor temperature be 50 DEG C, pressure be 30Mpa under conditions of carry out homogeneous, homogeneous 5 times;
Lyophilization:By the mixed liquor after homogeneous under the conditions of -20 DEG C, 6h is freezed, then presses 12kg/m in useful load2、 It is dry under conditions of operating pressure 80Pa, parsing pressure 20Pa, 40 DEG C of temperature, mixed material is made;
Crush:Dried mixed material is crushed, particle diameter is 500 μm, and mixed material powder is made;
It is prepared by health-care paste:The extracting solution of 3kg is added into 10kg mixed material powder, is deployed into paste, pearl is made in sizing Dish health-care paste.
The above is only the preferred embodiments of the present invention, and not makees limitation in any form to the present invention, Every technical spirit according to the present invention belongs to any simple modification made for any of the above embodiments, equivalent variations and modification In the range of technical solution of the present invention.

Claims (8)

  1. A kind of 1. processing method of loosestrife health-care paste, it is characterised in that the processing method of the loosestrife health-care paste include with Under several steps:
    Loosestrife processing:The loosestrife of no disease and pests harm is chosen, cleans up the Vitamin C that 15kg is added in backward 10kg loosestrives Acid sodium solution is beaten, and pearl puree is made, and the cellulase of 0.008kg, 0.005kg are added into 10kg pearl purees Pectase, 1.5h is digested in the environment of 50 DEG C, and pearl vegetable juice is made, spare;
    Myrobalan fruit is handled:The malic acid solution of 10kg, myrobalan fruit immersion 3h are added into the 10kg myrobalan fruit after fresh, cleaning After be beaten, myrobalan pulp is made;
    It is prepared by extracting solution:Win to be placed in the selenium aqueous solution of 10kg after the fresh folium cortex eucommiae clear water of 10kg elutes and extracted, extraction temperature Degree control is 60 DEG C, time control 30min, repeats extraction 5 times, extract is mixed, original is concentrated into the environment of 60 DEG C The 24% of volume, is made bark of eucommia leaf extract and folium cortex eucommiae filter residue;
    Extraction of the pumpkin into liquid separation:By cushaw leaf powder, with 1:7~1:17 water mixing, 1~10min is homogenized through historrhexis's machine Afterwards, then with solid-liquid ratio 1:30~1:70,200~800W of ultrasonic power, enzyme concentration cellulose enzyme amount 3000~5000U/g pumpkins Leaf dried powder, xylosidase 500~1500U/g pumpkin leaf dried powders, pH value 5.5~7.0, under the conditions of 40~65 DEG C enzymolysis and extraction 1~ 2h;Wherein, cellulose enzyme amount is 3~12 than xylose enzyme amount multiple;Obtained mixed liquor will be extracted through two kinds of different retention molecules The ultrafiltration membrance filter removal of impurities decoloration of amount, retaining molecular weight 18000~27000 used in first time, obtains cushaw leaf polysaccharide extraction liquid; Second of retaining molecular weight 7000~8000 used, second of ultra-filter retentate add as the raw material for preparing cushaw leaf polypeptide Enzyme amount 80~280U/g of papain, its pumpkin leaf dried powder enzyme activity 30U/mg, pepsin 50~150U/g pumpkin leaf dried powders More than enzyme activity 120U/mg, pH value 5.5~7.0,3~7h of enzymolysis and extraction, obtains protein enzymatic hydrolyzate under the conditions of 40~65 DEG C;Wherein, Papain enzyme amount is 2.0~7.0 than stomach cardia enzyme amount multiple;By polysaccharide extraction liquid and protein enzymatic hydrolyzate, respectively by nanofiltration Film is concentrated, and wherein the molecular cut off of film is 200~300,0.20~0.25Mpa of outlet pressure, and concentrated solution density is 1.15~1.20g/mL;Concentrate obtains pumpkin leaf polyose and cushaw leaf polypeptide, vacuum freeze drying bar through vacuum freeze drying again Part is that liquid is cooled to -25~-45 DEG C, and hothouse vacuum is 0.088~0.12MPa, 8~12mm of material thickness, heating plate temperature 25~40 DEG C of degree, condenser temperature -35~-62 DEG C, after freeze-drying, obtain pumpkin into liquid separation;
    Mixing:Take pearl vegetable juice 5kg, myrobalan's pulp 2kg, folium cortex eucommiae filter residue 2kg, pumpkin to be uniformly mixed into liquid separation 1.5kg, be made Mixed liquor.
  2. 2. the processing method of loosestrife health-care paste as claimed in claim 1, it is characterised in that after blend step, also need to carry out:
    Homogeneous:By mixed liquor temperature be 50 DEG C, pressure be 30Mpa under conditions of carry out homogeneous, homogeneous 5 times;
    Lyophilization:By the mixed liquor after homogeneous under the conditions of -20 DEG C, 6h is freezed, then presses 12kg/m in useful load2, work pressure It is dry under conditions of power 80Pa, parsing pressure 20Pa, 40 DEG C of temperature, mixed material is made;
    Crush:Dried mixed material is crushed, particle diameter is 500 μm, and mixed material powder is made;
    It is prepared by health-care paste:The extracting solution of 3kg is added into 10kg mixed material powder, is deployed into paste, loosestrife guarantor is made in sizing Strong cream.
  3. 3. the processing method of loosestrife health-care paste as claimed in claim 1, it is characterised in that the sodium ascorbate solution concentration For 5%.
  4. 4. the processing method of loosestrife health-care paste as claimed in claim 1, it is characterised in that the concentration of the malic acid solution is 20%.
  5. 5. the processing method of loosestrife health-care paste as claimed in claim 1, it is characterised in that the concentration of the selenium aqueous solution is 7%.
  6. 6. the processing method of loosestrife health-care paste as claimed in claim 1, it is characterised in that for pumpkin into liquid separation, cushaw leaf is more Sugar:Total sugar content >=88%, moisture≤5.0%;Cushaw leaf polypeptide:Content of peptides >=75%, moisture≤5.0%.
  7. 7. the processing method of loosestrife health-care paste as claimed in claim 1, it is characterised in that pumpkin is into need to be into before the extraction of liquid separation OK:By pick after annual pumpkin result to October fresh pumpkin leaf after impurity elimination is handled, processing is dried;Will be dry Dry cushaw leaf is carried out after suitably crushing with machinery, and it is spare to be placed in dry shady place.
  8. A kind of 8. loosestrife health-care paste of the processing method processing of loosestrife health-care paste as claimed in claim 1.
CN201810029187.6A 2018-01-12 2018-01-12 A kind of processing method of loosestrife health-care paste Pending CN108030094A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810029187.6A CN108030094A (en) 2018-01-12 2018-01-12 A kind of processing method of loosestrife health-care paste

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810029187.6A CN108030094A (en) 2018-01-12 2018-01-12 A kind of processing method of loosestrife health-care paste

Publications (1)

Publication Number Publication Date
CN108030094A true CN108030094A (en) 2018-05-15

Family

ID=62099272

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201810029187.6A Pending CN108030094A (en) 2018-01-12 2018-01-12 A kind of processing method of loosestrife health-care paste

Country Status (1)

Country Link
CN (1) CN108030094A (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2760366A1 (en) * 1997-03-04 1998-09-11 Harbex Limited Powdered sweet pumpkin hydrolysate preparation in high yield
CN104762356A (en) * 2015-04-08 2015-07-08 西北农林科技大学 Pumpkin leaf polysaccharide and polypeptide and preparation method thereof
CN106136233A (en) * 2016-06-30 2016-11-23 彭波 A kind of processing method of Fructus Piperis Longi health-care paste
CN106212797A (en) * 2016-08-25 2016-12-14 明毅强 The processing method of Herba Lysimachiae Clethroids (Radix Seu Herba Lysimachiae Clethroidis) health-care fruit caffein
CN106616582A (en) * 2016-09-29 2017-05-10 芜湖市三山区绿色食品产业协会 Processing method of clethra loosestrife root or herb health-care paste

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2760366A1 (en) * 1997-03-04 1998-09-11 Harbex Limited Powdered sweet pumpkin hydrolysate preparation in high yield
CN104762356A (en) * 2015-04-08 2015-07-08 西北农林科技大学 Pumpkin leaf polysaccharide and polypeptide and preparation method thereof
CN106136233A (en) * 2016-06-30 2016-11-23 彭波 A kind of processing method of Fructus Piperis Longi health-care paste
CN106212797A (en) * 2016-08-25 2016-12-14 明毅强 The processing method of Herba Lysimachiae Clethroids (Radix Seu Herba Lysimachiae Clethroidis) health-care fruit caffein
CN106616582A (en) * 2016-09-29 2017-05-10 芜湖市三山区绿色食品产业协会 Processing method of clethra loosestrife root or herb health-care paste

Similar Documents

Publication Publication Date Title
CN101617787B (en) Process for continuously extracting a plurality of products from highland barley
CN103960659B (en) Peony plant protein health care beverage and preparation method thereof
CN109793856B (en) Preparation method of dendrobium officinale powder
CN112971030A (en) Selenium-rich high-dietary-fiber recombinant rice and processing method thereof
CN102357212B (en) Compound product obtained through supercritical CO2 extraction, and preparation method thereof
CN107518407A (en) A kind of preparation method of dendrobium candidum health drink
CN104489837A (en) Cannabis protein peptide drink and preparation method thereof
CN108753894A (en) A kind of extracting method of American Ginseng peptide
CN106036902B (en) A kind of preparation method of quinoa leaf flavonoids health care buccal tablets
CN104996715A (en) A processing method for preparing wheat germ polypeptide by a compound fermentation method
CN106262105A (en) A kind of processing method of vacuum lyophilization Fructus Cucurbitae moschatae powder
CN108371316B (en) Preparation method of edible mushroom nano seasoning powder
CN106723026A (en) A kind of Barley Greeg and its preparation technology
CN102860450A (en) Compound nutrition powder rich in kudzu root cellulose oligosaccharide and preparation method thereof
CN107095214A (en) A kind of Rosa roxburghii Siberian solomonseal rhizome health-care powder and preparation method thereof
CN104762356B (en) A kind of pumpkin leaf polyose and polypeptide and preparation method thereof
CN106190706A (en) A kind of brewage process of beech tree HUADOU Folium pyri tea wine
CN106108003A (en) A kind of preparation method rich in the black ginseng of rare ginsenoside Rh2
CN105831775A (en) Method for improving soluble meal fiber content of lotus rhizome slag
CN102742854A (en) New process for extracting dietary fibers from corn germ meal
CN107836562A (en) A kind of peony seeds active peptide and preparation method
CN105012778A (en) Dendrobium officinale-coix seed capsules and preparation method thereof
CN105031289A (en) Dendrobium officinale and lucid ganoderma capsules and preparation method thereof
CN111713622A (en) Processing method of concentrated pueraria juice
CN101766305A (en) Corn filament high-nutrition health-care food and preparation method

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20180515

RJ01 Rejection of invention patent application after publication