CN108026150A - 小麦秆锈病抗性基因及使用方法 - Google Patents

小麦秆锈病抗性基因及使用方法 Download PDF

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CN108026150A
CN108026150A CN201680054513.8A CN201680054513A CN108026150A CN 108026150 A CN108026150 A CN 108026150A CN 201680054513 A CN201680054513 A CN 201680054513A CN 108026150 A CN108026150 A CN 108026150A
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E·拉古达
S·佩里亚纳恩
B·斯特欧尔纳扎尔
K·威特科
B·沃尔夫
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Abstract

本发明提供了用于增强小麦植物抵抗由小麦秆锈病菌引起的小麦秆锈病的抗性的组合物和方法。所述组合物包含编码抗性(R)基因产物及其变体的核酸分子，以及包含所述核酸分子的植物、种子和植物细胞。用于增强小麦植物抵抗小麦秆锈病的抗性的方法包括将编码R基因产物的核酸分子引入小麦植物细胞中。本发明另外提供在农业中使用所述小麦植物以限制小麦秆锈病的方法。

Description

小麦秆锈病抗性基因及使用方法
相关申请的交叉引用
本申请要求2015年8月4日提交的美国临时专利申请号62/200,894的权益，该申请以引用方式全部并入本文。
参照以文本文件提交的序列表
序列表的正式文本作为ASCII格式的序列表通过EFS-Web以电子方式提交，文件名为070294-0101SEQLST.TXT，于2016年7月29日创建，并且大小为711千字节，而且与说明书同时提交。在该ASCII格式的文件中所包含的序列表为说明书的一部分，并且以引用方式全文并入本文。
技术领域
本发明涉及基因分离和植物改良领域，特别涉及通过使用疾病抗性基因增强植物抵抗植物疾病的抗性。
背景技术
在全世界小麦生产中，植物疾病导致显著的产量损失。锈病是最具破坏性的小麦疾病的一种。由小麦秆锈病菌(Puccinia graminis f.sp.tritici)引起的小麦秆锈病是当今影响小麦生产的最毁灭性的疾病之一。尽管已经证明包含抵抗小麦秆锈病菌的抗性基因(R)的小麦植物有效地限制由小麦秆锈病引起的农业损失，但是近期出现了小麦秆锈病菌的新物种，而所述R基因对其是无效的。虽然杀虫剂可以用于控制小麦秆锈病，可是杀虫剂是昂贵的并且与农业的可持续强化不一致，而且在发展中国家，简而言之，杀虫剂是自给农民负担不起的。
农业的可持续强化要求增加使用遗传解决方法，替代化学解决方法(例如杀虫剂)来保护农作物抵抗病原体和害虫(Jones et al.(2014)Philos.T.Roy.Soc.B 369:20130087)。驯化的农作物(例如小麦)的野生近源种包含巨大多样性的可用的R基因，其为用于可持续性疾病控制的有价值的来源。然而，用于引入R基因的传统方法通常涉及长的育种时间线，以破坏与其他基因的有害等位基因的连锁。此外，当一次部署一个R基因时，在几个季节内可以克服R基因(McDonald and Linde(2002)Annu.Rev.Phytopathol.40:349-379)。但是，分子克隆可以避免连锁累赘并同时引入多个R基因(Dangl et al.(2013)Science 341:746-751)，这可以延迟抗性突破病原体物种快速进化，由此提供更持久的抗性(McDonald and Linde(2002)Annu.Rev.Phytopathol.40:349-379)。
尽管传统的基于图谱的克隆法已经用于分离植物的R基因，但是许多植物基因组携带大的染色体区域，这些区域由于抑制重组而使得传统的基于图谱的克隆法无法达到(Gaut et al.(2007)Nature Rev.Genet.8:77-84)，并且小麦也不例外。因此，不依赖于重组的新的补充方法必须用于鉴定农作物植物和它们的非驯化的近源种中的其他R基因。
发明概述
本发明提供了已知赋予植物以抵抗引起小麦秆锈病的至少一种病原体菌株(小麦秆锈病菌)的抗性的抗性(R)基因的核酸分子。在一个实施方案中，本发明提供了包含R基因Sr22及其变体(例如包括直系同源物和非天然形成的变体)的核酸分子。在另一个实施方案中，本发明提供了包含R基因Sr45及其变体(例如包括直系同源物和非天然形成的变体)的核酸分子。
本发明进一步提供了在其基因组中包含本发明的一个或多个核苷酸构建体的植物、植物细胞和种子。所述多核苷酸构建体包含编码本发明的抗性(R)蛋白质的核苷酸序列。此类R蛋白质由本发明的R基因编码。在优选的实施方案中，植物和种子是使用本发明的一个或多个核苷酸构建体转化的转基因小麦植物和种子。优选地，与不包含所述多核苷酸构建体的对照小麦植物的抗性相比，此类小麦植物包含抵抗引起小麦秆锈病的至少一种病原体菌株(小麦秆锈病菌)的增强的抗性。
本发明提供了用于增强小麦植物抵抗小麦秆锈病的抗性的方法。此类方法包括将包含本发明的R基因的核苷酸序列的多核苷酸构建体引入至少一种小麦植物细胞中。在一些实施方案中，所述多核苷酸构建体或其部分被稳定地引入植物细胞的基因组中，并且在其他的实施方案中，所述多核苷酸构建体不稳定地引入小麦植物细胞的基因组中。用于增强小麦植物抵抗小麦秆锈病的抗性的方法可以任选地进一步包括使小麦植物细胞再生成在其基因组中包含所述多核苷酸构建体的小麦植物。优选地，相对于对照小麦植物而言，此类小麦植物包含抵抗由小麦秆锈病菌的至少一个物种引起的小麦秆锈病的增强的抗性。
本发明另外提供了用于鉴定小麦植物的方法，所述小麦植物展现出抵抗小麦秆锈病的新赋予的或增强的抗性。所述方法包括检测小麦植物中选自Sr22和Sr45的至少一个R基因的存在情况。
本发明还提供了在农业农作物生产中使用本发明的小麦植物限制小麦秆锈病的方法。所述方法包括种植通过本发明的小麦植物生产的小麦种子，其中所述小麦种子包含本发明的至少一个R基因核苷酸序列。所述方法进一步包括使小麦植物在有利用于小麦植物生长和发育的条件下生长，并且任选地由所述小麦植物收获至少一个种子。
本发明另外提供了包含本发明的一个或多个核酸分子的植物、植物部分、种子、植物细胞、其他宿主细胞、表达盒和载体。
附图简述
图1.Schomburgk(Sr22)、EMS-诱导的易感突变体和易感对照Morocco的秆锈病感染表型。
图2.CS1D5406(Sr45)、EMS-诱导的易感突变体和易感对照Chinese Spring的秆锈病感染表型。
图3.在六倍体小麦(普通小麦(Triticum aestivum))中，在Sr22周围的标志物的连锁。
图4.在六倍体小麦中，在Sr45周围的标志物的连锁。
图5.通过测序EMS-诱导的易感突变体来克隆Sr22。Sr22的示意图显示内含子-外显子边界、蛋白质结构域结构(染色的框)、5'和3'UTR(白色的框)、以及错义和无义(*)突变的位置。通过RenSeq获得的Sr22和Sr45重叠群(contig)用灰色显示。3'重叠群被鉴定为由突变体的序列比较而得到的最佳候选物。3'和5'重叠群通过Sr22RNAseq读值以及通过NCBI中全长NB-LRR的BLAST桥接。重叠群通过局部RenSeq和染色体步移进行延伸，并且通过PCR扩增并对得自基因组DNA的7.815kb片段的测序证明全长Sr22序列。
图6.通过测序EMS-诱导的易感突变体来克隆Sr45。Sr45的示意图显示内含子-外显子边界、蛋白质结构域结构(染色的框)、5'和3'UTR(白色的框)以及错义和无义(*)突变的位置。通过RenSeq获得的Sr45重叠群用灰色显示。3'重叠群被鉴定为由突变体的序列比较得到的最佳候选物。
图7.由二倍体(A基因组)和六倍体小麦鉴定的Sr22基因变体的邻接树分析。基于对小麦秆锈病的抗性假设的Sr22的存在或缺乏分别以“+”或“-”记号表示，在框、三角或圆内分别表示野生一粒小麦(T.boeoticum)、栽培一粒小麦(T.monococcum)或普通小麦起源。
图8.由Schomburgk得到的SR22的氨基酸序列(SEQ ID NO:2)。突出显示的结构域为使用SMART(可以在互联网上得到：smart.embl-heidelberg.de)预测的卷曲螺旋结构域(黑体；氨基酸120-147)、核苷酸结合结构域(下划线；氨基酸178-465)和富含亮氨酸重复结构域(斜体；氨基酸601-853)。
图9.由CS1D5406得到的SR45的氨基酸序列(SEQ ID NO:50)。突出显示的结构域为使用SMART(可以在互联网上得到：smart.embl-heidelberg.de)预测的卷曲螺旋结构域(黑体；氨基酸32-55)、核苷酸结合结构域(下划线；氨基酸175-452)和富含亮氨酸重复结构域(斜体；氨基酸578-867)。
图10A-10P.由以下二倍体和六倍体小麦得到的Sr22氨基酸序列的多重比对：PI573523(SEQ ID NO:14)、IG44878(SEQ ID NO:29)、DV92(SEQ ID NO:38)、PI289605(SEQID NO:8)、PI330550(SEQ ID NO:11)、PI190945(SEQ ID NO:5)、W3534(SEQ ID NO:35)、IG44857(SEQ ID NO:23)、IG44921(SEQ ID NO:26)、IG44855(SEQ ID NO:20)、Schomburgk(SEQ ID NO:2)、PI355523(SEQ ID NO:17)、PI272557(SEQ ID NO:32)和Westonia(SEQ IDNO:41)。
图11A-11E全部为多面板图，其示出由Fielder的秆锈病抗性测试得到的结果，其中所述Fielder使用得自Schomburgk的Sr22、得自PI190945的Sr22以及Sr45进行转化。在图11A-11E的各图中，白色标尺对应于10mm。
图11A示出使用构建体PC103转化的易感栽培品种Fielder的结果，其中所述构建体PC103包含得自Schomburgk(野生一粒小麦)的Sr22以及天然Sr22的5’和3’调控元件。图片示出6种转基因植物(编号PC103-1、-2、-3、-4、-5、-6)。发现所有的植物都对澳大利亚秆锈病物种98-1,2,3,5和6具有抗性，类似于栽培品种Schomburgk(感染型2-)中的Sr22应答。在图像中，泳道1为Fielder(易感的对照)，而用泳道2至7分别为转基因系1至6。
图11B示出使用构建体PC129转化的易感栽培品种Fielder的结果，其中所述构建体PC129包含得自PI190945(栽培一粒小麦)的Sr22以及天然的5’和3’调控元件。回收总计13种转基因植物，并针对澳大利亚秆锈病物种98-1,2,3,5和6的抗性进行评分。植物编号PC129-1、-4、-6、-7、-8、-10、-11、-13具有与PI190945(感染型2-)中的Sr22应答相似的抗性。所有这8种植物中，PC129-1、-4、-6、-11和-13示于图像中。一种植物(PC129-9)具有感染型2(稍微高于PI190945)，而3种植物(PC129-2、-5和-12)具有感染型2+(高于PI190945)。一种植物(PC129-3)具有感染型3+，其类似于Fielder。
图11C示出使用构建体PC110转化的易感栽培品种Fielder的结果，其中所述构建体PC110包含得自节节麦(Aegilops tauschii)编号AUS18911的Sr45以及天然的5’和3’调控元件。回收总计12种转基因植物，并针对澳大利亚秆锈病物种98-1,2,3,5和6的抗性进行评分。7种植物(PC110-1、-2、-4、-5、-7、-10、-12)具有感染型1，其类似于AUS18911和CS1D5406中的Sr45。4种植物(PC110-3、-6、-9、-11)具有感染型1(稍微高于AUS18911)。一种植物(PC110-8)具有感染型3+，其类似于Fielder。
图11D-11E示出转基因植物的结果，所述转基因植物包含得自Schomburgk的Sr22、得自PI190945的Sr22和得自PI573523的Sr22以及天然的或非天然的调控元件。使用构建体PC126、PC127、PC128、PC130、PC131、PC132、PC146和PC147转化易感栽培品种Fielder。各构建体的启动子、编码区和终止子列于表6中。对于各构建体而言，针对澳大利亚秆锈病物种98-1,2,3,5和6的抗性，对10种转基因植物进行评分。在图11D中，衍生自PC126、PC127、PC128、PC130和PC132的植物均具有抗性，并且感染型范围为1+至2，而衍生自PC131的植物均是易感的，并且感染型为3+。各构建体的代表性植物示于图片中。在图11E中，衍生自PC146的所有植物均是高度易感的，而衍生自PC147的植物(除了系列9)产生感染型1，是Sr45的强抗性反应的典型。
序列表
使用核苷酸碱基的标准字母缩写和氨基酸的3字母密码示出在所附的序列表中列出的核苷酸和氨基酸序列。核苷酸序列符合在序列5'末端开始，向前进行(即，在各行的左至右)至3'末端的标准惯例。仅示出各核苷酸序列的一条链，但是应该理解通过对所显示的链的任何参照而包含互补链。氨基酸序列符合在序列的氨基末端开始，向前进行(即，在各行的左至右)至羧基末端的标准惯例。
SEQ ID NO:1列出得自普通小麦“Schomburgk”的R基因Sr22的核苷酸序列。
SEQ ID NO:2列出由得自普通小麦“Schomburgk”的Sr22编码的R蛋白质的氨基酸序列。
SEQ ID NO:3列出得自普通小麦“Schomburgk”的Sr22的cDNA的编码区的核苷酸序列。如果需要，终止密码子(例如TAA、TAG或TGA)与包含SEQ ID NO:3的核酸分子的3'末端可操作地连接。
SEQ ID NO:4列出得自栽培一粒小麦编号PI190945的R基因Sr22的核苷酸序列。
SEQ ID NO:5列出由得自栽培一粒小麦编号PI190945的Sr22编码的R蛋白质的氨基酸序列。
SEQ ID NO:6列出得自栽培一粒小麦编号PI190945的Sr22的cDNA的编码区的核苷酸序列。如果需要，终止密码子(例如TAA、TAG或TGA)与包含SEQ ID NO:6的核酸分子的3'末端可操作地连接。
SEQ ID NO:7列出得自栽培一粒小麦编号PI289605的R基因Sr22的核苷酸序列。
SEQ ID NO:8列出由得自栽培一粒小麦编号PI289605的Sr22编码的R蛋白质的氨基酸序列。
SEQ ID NO:9列出得自栽培一粒小麦编号PI289605的Sr22的cDNA的编码区的核苷酸序列。如果需要，终止密码子(例如TAA、TAG或TGA)与包含SEQ ID NO:9的核酸分子的3'末端可操作地连接。
SEQ ID NO:10列出得自栽培一粒小麦编号PI330550的R基因Sr22的核苷酸序列。
SEQ ID NO:11列出由得自栽培一粒小麦编号PI330550的Sr22编码的R蛋白质的氨基酸序列。
SEQ ID NO:12列出得自栽培一粒小麦编号PI330550的Sr22的cDNA的编码区的核苷酸序列。如果需要，终止密码子(例如TAA、TAG或TGA)与包含SEQ ID NO:12的核酸分子的3'末端可操作地连接。
SEQ ID NO:13列出得自栽培一粒小麦编号PI573523的抗性基因类似物的核苷酸序列。该抗性基因类似物为得自Schomburgk的Sr22的类似物。
SEQ ID NO:14列出由抗性基因类似物编码的蛋白质的氨基酸序列，其中所述抗性基因类似物包含SEQ ID NO:13中列出的核苷酸序列。
SEQ ID NO:15列出由抗性基因类似物的编码区的核苷酸序列，其中所述抗性基因类似物包含SEQ ID NO:13中列出的核苷酸序列。如果需要，终止密码子(例如TAA、TAG或TGA)与包含SEQ ID NO:15的核酸分子的3'末端可操作地连接。
SEQ ID NO:16列出得自栽培一粒小麦编号PI355523的抗性基因类似物的核苷酸序列。该抗性基因类似物为得自Schomburgk的Sr22的类似物。
SEQ ID NO:17列出由抗性基因类似物编码的蛋白质的氨基酸序列，其中所述抗性基因类似物包含SEQ ID NO:16中列出的核苷酸序列。
SEQ ID NO:18列出抗性基因类似物的编码区的核苷酸序列，其中所述抗性基因类似物包含SEQ ID NO:16中列出的核苷酸序列。如果需要，终止密码子(例如TAA、TAG或TGA)与包含SEQ ID NO:18的核酸分子的3'末端可操作地连接。
SEQ ID NO:19列出得自野生一粒小麦编号IG44855的R基因Sr22的核苷酸序列。
SEQ ID NO:20列出由得自野生一粒小麦编号IG44855的Sr22编码的R蛋白质的氨基酸序列。
SEQ ID NO:21列出得自野生一粒小麦编号IG44855的Sr22的cDNA的编码区的核苷酸序列。如果需要，终止密码子(例如TAA、TAG或TGA)与包含SEQ ID NO:21的核酸分子的3'末端可操作地连接。
SEQ ID NO:22列出得自野生一粒小麦编号IG44857的R基因Sr22的核苷酸序列。
SEQ ID NO:23列出由得自野生一粒小麦编号IG44857的Sr22编码的R蛋白质的氨基酸序列。
SEQ ID NO:24列出得自野生一粒小麦编号IG44857的Sr22的cDNA的编码区的核苷酸序列。如果需要，终止密码子(例如TAA、TAG或TGA)与包含SEQ ID NO:24的核酸分子的3'末端可操作地连接。
SEQ ID NO:25列出得自野生一粒小麦编号IG44921的R基因Sr22的核苷酸序列。
SEQ ID NO:26列出由得自野生一粒小麦编号IG44921的Sr22编码的R蛋白质的氨基酸序列。
SEQ ID NO:27列出得自野生一粒小麦编号IG44921的Sr22的cDNA的编码区的核苷酸序列。如果需要，终止密码子(例如TAA、TAG或TGA)与包含SEQ ID NO:27的核酸分子的3'末端可操作地连接。
SEQ ID NO:28列出得自野生一粒小麦编号IG44878的抗性基因类似物的核苷酸序列。该抗性基因类似物为得自Schomburgk的Sr22的类似物。
SEQ ID NO:29列出由抗性基因类似物编码的蛋白质的氨基酸序列，其中所述抗性基因类似物包含SEQ ID NO:28中列出的核苷酸序列。
SEQ ID NO:30列出抗性基因类似物的编码区的核苷酸序列，其中所述抗性基因类似物包含SEQ ID NO:28中列出的核苷酸序列。如果需要，终止密码子(例如TAA、TAG或TGA)与包含SEQ ID NO:30的核酸分子的3'末端可操作地连接。
SEQ ID NO:31列出得自栽培一粒小麦编号PI272557的抗性基因类似物的核苷酸序列。该抗性基因类似物为得自Schomburgk的Sr22的类似物。
SEQ ID NO:32列出由抗性基因类似物编码的蛋白质的氨基酸序列，其中所述抗性基因类似物包含SEQ ID NO:31中列出的核苷酸序列。
SEQ ID NO:33列出抗性基因类似物的编码区的核苷酸序列，其中所述抗性基因类似物包含SEQ ID NO:31中列出的核苷酸序列。如果需要，终止密码子(例如TAA、TAG或TGA)与包含SEQ ID NO:33的核酸分子的3'末端可操作地连接。
SEQ ID NO:34列出得自普通小麦编号W3534的R基因Sr22的核苷酸序列。
SEQ ID NO:35列出由得自普通小麦编号W3534的Sr22编码的R蛋白质的氨基酸序列。
SEQ ID NO:36列出得自普通小麦编号W3534的Sr22的cDNA的编码区的核苷酸序列。如果需要，终止密码子(例如TAA、TAG或TGA)与包含SEQ ID NO:36的核酸分子的3'末端可操作地连接。
SEQ ID NO:37列出得自栽培一粒小麦编号DV92的抗性基因类似物的核苷酸序列。该抗性基因类似物为得自Schomburgk的Sr22的类似物。
SEQ ID NO:38列出由抗性基因类似物编码的蛋白质的氨基酸序列，其中所述抗性基因类似物包含SEQ ID NO:37中列出的核苷酸序列。
SEQ ID NO:39列出抗性基因类似物的编码区的核苷酸序列，其中所述抗性基因类似物包含SEQ ID NO:37中列出的核苷酸序列。如果需要，终止密码子(例如TAA、TAG或TGA)与包含SEQ ID NO:39的核酸分子的3'末端可操作地连接。
SEQ ID NO:40列出得自普通小麦‘Westonia’的抗性基因类似物的核苷酸序列。该抗性基因类似物为得自Schomburgk的Sr22的类似物。
SEQ ID NO:41列出由抗性基因类似物编码的蛋白质的氨基酸序列，其中所述抗性基因类似物包含SEQ ID NO:40中列出的核苷酸序列。
SEQ ID NO:42列出抗性基因类似物的编码区的核苷酸序列，其中所述抗性基因类似物包含SEQ ID NO:40中列出的核苷酸序列。如果需要，终止密码子(例如TAA、TAG或TGA)与包含SEQ ID NO:42的核酸分子的3'末端可操作地连接。
SEQ ID NO:43列出得自普通小麦“Schomburgk”的Sr22的2S突变体的核苷酸序列。
SEQ ID NO:44列出得自普通小麦“Schomburgk”的Sr22的7S1突变体的核苷酸序列。
SEQ ID NO:45列出得自普通小麦“Schomburgk”的Sr22的1S突变体的核苷酸序列。
SEQ ID NO:46列出得自普通小麦“Schomburgk”的Sr22的3S1突变体的核苷酸序列。
SEQ ID NO:47列出得自普通小麦“Schomburgk”的Sr22的5S1突变体的核苷酸序列。
SEQ ID NO:48列出得自普通小麦“Schomburgk”的Sr22的6S突变体的核苷酸序列。
SEQ ID NO:49列出得自节节麦编号AUS18911的R基因Sr45的核苷酸序列。
SEQ ID NO:50列出得自节节麦编号AUS18911的Sr45编码的R蛋白质的氨基酸序列。
SEQ ID NO:51列出得自节节麦编号AUS18911的Sr45的cDNA的编码区的核苷酸序列。如果需要，终止密码子(例如TAA、TAG或TGA)与包含SEQ ID NO:51的核酸分子的3'末端可操作地连接。
SEQ ID NO:52列出普通小麦代换系CS1D5406的52S1突变体的核苷酸序列。
SEQ ID NO:53列出普通小麦代换系CS1D5406的57S1突变体的核苷酸序列。
SEQ ID NO:54列出普通小麦代换系CS1D5406的59S1突变体的核苷酸序列。
SEQ ID NO:55列出普通小麦代换系CS1D5406的49S1突变体的核苷酸序列。
SEQ ID NO:56列出普通小麦代换系CS1D5406的55S1突变体的核苷酸序列。
SEQ ID NO:57列出普通小麦代换系CS1D5406的61S1突变体的核苷酸序列。
SEQ ID NO:58-137为下文实施例中描述的寡核苷酸序列。
SEQ ID NO:138列出实施例13中描述的启动子玉米泛素构建体的核苷酸序列。
SEQ ID NO:139列出实施例13中描述的从节节麦得到的Sr33衍生的启动子Sr33天然构建体的核苷酸序列。
SEQ ID NO:140列出实施例13中描述的从栽培一粒小麦编号PI190945的Sr22衍生的启动子PI190945天然构建体的核苷酸序列。
SEQ ID NO:141列出实施例13中描述的从普通小麦“Schomburgk”的Sr22衍生的编码区-1Sr22基因座构建体的核苷酸序列。
SEQ ID NO:142列出实施例13中描述的从普通小麦“Schomburgk”的Sr22衍生的编码区-2Sr22基因座驯化的BsaI构建体的核苷酸序列。
SEQ ID NO:143列出实施例13中描述的从栽培一粒小麦编号PI190945的Sr22衍生的编码区-1PI190945构建体的核苷酸序列。
SEQ ID NO:144列出实施例13中描述的从栽培一粒小麦编号PI190945的Sr22基因座衍生的编码区-2PI190945驯化的BsaI构建体的核苷酸序列。
SEQ ID NO:145列出实施例13中描述的从栽培一粒小麦编号PI573523的Sr22衍生的编码区-1PI573523构建体的核苷酸序列。
SEQ ID NO:146列出实施例13中描述的从栽培一粒小麦编号PI573523的Sr22衍生的编码区-2PI573523驯化的BsaI构建体的核苷酸序列。
SEQ ID NO:147列出实施例13中描述的从节节麦的Sr33衍生的终止子Sr33天然构建体的核苷酸序列。
SEQ ID NO:148列出实施例13中描述的从普通小麦“Schomburgk”的Sr22衍生的终止子Schomburgk驯化的BsaI构建体的核苷酸序列。
SEQ ID NO:149列出实施例13中描述的从栽培一粒小麦编号PI190945的Sr22衍生的终止子PI190945驯化的BsaI构建体的核苷酸序列。
SEQ ID NO:150列出实施例13中描述的从节节麦编号AUS18911的Sr45衍生的编码区Sr45基因座驯化的BsaI构建体的核苷酸序列。
SEQ ID NO:151列出实施例13中描述的从全基因Sr33启动子Sr22编码区Sr33天然终止子构建体的核苷酸序列。
SEQ ID NO:152-162列出在下表6中中描述的构建体的核苷酸序列。
SEQ ID NO:163列出双元载体pVecBARII的核苷酸序列，其还称为VecBARII。
发明详述
现在参照附图，下文将更全面地描述本发明，其中示出本发明的一些实施方案，但不是全部的实施方案。事实上，这些发明可以以多种不同的形式体现，并且不应该解释成限制于本发明列出的实施方案，而且提供这些实施方案，使得本发明公开满足适用的法律需要。在全文中，相同的数字是指相同的元件。
本文中列出的本发明的许多修改和其他的实施方案提醒本发明所属技术领域的任一技术人员，在上述说明书和相关的附图中呈现的教导的益处。因此，应该理解的是本发明不限于所公开的具体的实施方案，并且修改和其他的实施方案应该被包含在所附权利要求书的范围内。尽管在本发明中使用了具体的术语，但是它们仅是一般的且描述意义上的使用，不是为了限定的目的。
本发明涉及植物抗性(R)基因的分离，特别是赋予小麦植物以抵抗由小麦秆锈病菌引起的小麦秆锈病的R基因。如下文所公开，使用快速克隆R基因的3步方法来分离R基因，Sr22和Sr45。称为“RenSeq+EMS”的方法涉及(i)化学诱变和针对易感突变体的筛选，(ii)外显子捕获和下一代测序；然后(iii)野生型与突变体的序列比较。RenSeq+EMS方法在均于2015年2月20日提交的美国专利申请号14/667,116和国际专利申请PCT/US2015/016792中公开。
本发明提供了包含R基因的核苷酸序列，特别是Sr22和Sr45的核苷酸序列，以及其天然形成的(例如直系同源物和等位基因变体)和合成的或人工的(即，非天然形成的)变体的核酸分子。这种R基因的核苷酸序列(在本发明中还称为R基因核苷酸序列)编码R蛋白质。本发明的R基因核苷酸序列包括但不限于野生型R基因(其包含天然启动子和包含编码区的3′相邻区)，cDNA序列，和仅包含编码区的核苷酸序列。这种R基因核苷酸序列的实例包括SEQ ID NO:1、3、4和6中列出的核苷酸序列及其变体。在其中天然R基因启动子不用于驱动编码R蛋白质的核苷酸序列的表达的实施方案中，异源启动子与编码本发明的R蛋白质的核苷酸序列可操作地连接，从而驱动编码R蛋白质的核苷酸序列在植物中表达。
优选地，本发明的R蛋白质为功能R蛋白质，其能够赋予包含R蛋白质的小麦植物以抵抗由小麦秆锈病菌的至少一个物种引起的小麦秆锈病的增强的抗性。在某些实施方案中，本发明的R蛋白质包括抵抗小麦秆锈病菌的多个物种的广谱抗性，例如Sr22和Sr45编码的R蛋白质。
本发明进一步提供了包含多核苷酸构建体的转基因植物，其中所述构建体包含本发明的R基因核苷酸序列。在一些实施方案中，多核苷酸构建体被稳定地引入至植物的基因组中，而在其他的实施方案中，通过瞬时转化方法转化植物，并且所述多核苷酸构建体不稳定地引入至植物的基因组中。用于植物的稳定的和瞬时转化的方法在本发明的其他处公开，或者在本领域中是已知的。在本发明的优选的实施方案中，转基因植物为小麦植物，其包含抵抗由小麦秆锈病菌的至少一个物种引起的小麦秆锈病的增强的抗性。
在某些实施方案中，本发明的转基因植物包含多核苷酸构建体，其包含编码R蛋白质的核苷酸序列和可操作地连接的用于表达编码R蛋白质的核苷酸序列的异源启动子。异源启动子的选择可以取决于多种因素，例如所需的时机、位置和表达方式，以及对特定生物或非生物刺激的应答。目的启动子包括但不限于病原体诱导型、构成型、组织优选型、伤口诱导型和化学调节型启动子。
在本发明的某些实施方案中，转基因植物，特别是转基因小麦植物，可以包含1个、2个、3个、4个、5个、6个或更多的编码R蛋白质的核苷酸序列。通常但是不必须，2个或更多的R蛋白质彼此不同。就本发明而言，当2种R蛋白质具有不一致的氨基酸序列时，R蛋白质不同于另一种R蛋白质。在本发明的某些实施方案中，用于小麦秆锈病的各种不同的R蛋白质在抗性特征方面具有一种或多种差异，例如抵抗不同物种和/或几组物种的小麦秆锈病菌的抗性。应该认识到通过将2个、3个、4个、5个、6个或更多的核苷酸序列与编码用于小麦秆锈病的不同R蛋白质的各个核苷酸序列组合，可以产生包含抵抗小麦秆锈病菌的多个物种的广谱抗性的小麦植物。这种小麦植物发现用于已知形成小麦秆锈病菌的多个物种的地区的农业中。
可以在单一小麦植物中与本发明的Sr22和/或Sr45核苷酸序列组合的小麦秆锈病R基因的实例包括Sr26、Sr32、Sr33(GenBank编号KF031299.1)、Sr35(GenBank编号KC573058.1)、Sr39、Sr40、Sr47、Sr50及成株抗性基因Sr57/Lr34(GenBank编号FJ436983.1)和Sr55/Lr67。
包含多重R基因的本发明的转基因植物可以通过使用包含本发明的R基因核苷酸序列(包括例如Sr22或Sr45核苷酸序列或其变体)的多核苷酸构建体转化已经包含一个或多个其他的R基因核苷酸序列的植物来产生。已经包含一个或多个其他的R基因核苷酸序列的此类植物可以包含对于基因组或植物而言是天然的R基因，其通过有性生殖被引入至植物中，或者通过使用R基因核苷酸序列转化植物或其祖先而被引入。备选地，可以通过例如转化或有性生殖将一个或多个其他的R基因核苷酸序列引入至本发明的转基因植物中，其已经包含本发明的多核苷酸构建体。
在其他的实施方案中，2个或更多个不同的R基因序列可以通过使用包含2个或更多个R基因核苷酸序列的多核苷酸构建体或载体稳定地转化植物而被引入至所述植物中。应该认识到这种方法对于植物育种而言是优选的，这是因为预计2个或更多个R基因核苷酸序列将紧密地连锁，因此隔离单一的基因座。备选地，可以使用在本发明中其他处描述的或者本领域已知的基于同源重组的基因组修饰方法将本发明的多核苷酸构建体以与另一个R基因核苷酸序列紧密相邻的方式引入至植物的基因组中。
本发明进一步提供了用于增强小麦植物抵抗小麦秆锈病的抗性的方法。所述方法包括将本发明的多核苷酸构建体引入至至少一种小麦植物细胞中。在某些实施方案中，多核苷酸构建体被稳定地引入至小麦植物细胞的基因组中。如果需要，所述方法可以进一步包括使植物细胞再生成在其基因组中包含所述多核苷酸构建体的小麦植物。优选地，相对于对照小麦植物抵抗由小麦秆锈病菌的至少一种物种引起的小麦秆锈病的抗性而言，这种再生的小麦植物包含抵抗由小麦秆锈病菌的至少一个物种引起的小麦秆锈病的增强的抗性。如果需要，所述方法可以进一步包括产生小麦植物，如上文所述，所述植物包含编码R蛋白质的1个、2个、3个、4个、5个、6个或更多个核苷酸序列，优选地每个核苷酸序列均编码不同的R蛋白质。
本发明公开的小麦植物在用于农业农作物生产(特别是在其中小麦秆锈病盛行的地区)中限制小麦秆锈病的方法中具有用途。本发明的方法包括种植由本发明的小麦植物产生的小麦种子，其中所述小麦种子包含本发明的至少一个R基因核苷酸序列。所述方法进一步包括使小麦植物在有利于小麦植物生长和发育的条件下生长，并且任选地，由小麦植物收获至少一个种子。
116本发明另外提供了用于鉴定小麦植物的方法，其中所述小麦植物展示出新赋予的或增强的抵抗小麦秆锈病的抗性。所述方法在育种用于抵抗小麦秆锈病的小麦植物中具有用途。这种抗性小麦植物在小麦种子的农业生产中具有用途。所述方法包括检测小麦植物中至少一种R基因的存在情况，其中所述R基因选自Sr22和Sr45。在本发明的一些实施方案中，检测R基因的存在情况包括检测由小麦植物分离的基因组DNA中的完整R基因。但是，在优选的实施方案中，检测R基因的存在情况包括检测R基因中至少一种标志物的存在情况。在本发明的其他实施方案中，检测R基因的存在情况包括使用例如免疫检测方法(涉及对R蛋白质具有特异性的抗体)检测由R基因编码的R蛋白质的存在情况。
在用于鉴定小麦植物的方法中，其中所述小麦植物展示出新赋予的或增强的抵抗小麦秀病菌的抗性，检测小麦中R基因的存在情况可以涉及在本发明的其他处公开的或本领域已知的以下分子生物学技术的一种或多种，包括但不限于由小麦植物分离基因组DNA和/或RNA，通过PCR扩增而扩增包含R基因和/或其标志物的核酸分子，对包含R基因和/或标志物的核酸分子测序，通过核酸杂交鉴定R基因、标志物或R基因的转录物，以及针对R基因编码的R蛋白质的检测实施免疫测定法。应该认识到寡核苷酸探针和PCR引物可以设计成鉴定本发明的R基因，并且这种探针和PCR引物可以用于本发明其他处公开的或本领域已知的方法中，用于快速鉴定小麦植物群体中一种或多种包含本发明的R基因的小麦植物。
根据所需的结果，本发明的多核苷酸构建体可以被稳定地引入至植物细胞的基因组中，或者不稳定地引入至植物细胞的基因组中。例如如果所需的结果是为了产生稳定转化的植物，该植物具有抵抗小麦秆锈病菌的至少一个物种引起的小麦秆锈病的增强的抗性，则所述多核苷酸构建体可以例如被融合至适用于将多核苷酸构建体稳定地引入植物细胞基因组中的植物转化载体中。通常，稳定转化的植物细胞将再生成在其基因组中包含所述多核苷酸构建体的转化的植物。这种稳定转化的植物能够通过有性和/或无性生殖在随后的传代中将所述多核苷酸构建体传送至后代植物中。植物转化的载体、使用引入的多核苷酸构建体稳定地转化植物的方法以及由转化的植物细胞和组织使植物再生的方法对于单子叶植物和双子叶植物而言是本领域公知的，或者在本发明的其他处有所描述。
本发明提供了包含R基因的核苷酸分子。优选地，这种R基因能够赋予宿主植物(特别是小麦植物)以抵抗引起小麦秆锈病的病原体(小麦秆锈病菌)的至少一个物种的增强的抗性。因此，这种R基因在农业生产中在限制由小麦秆锈病菌引起的小麦秆锈病中具有用途。本发明的R基因包括但不限于其核苷酸序列在本发明中公开的R基因，但还包括能够赋予小麦植物以抵抗由小麦秆锈病菌的至少一个物种引起的小麦秆锈病的抗性的直系同源物和其他的变体。本领域已知或本发明另外公开的方法用于确定植物抵抗由小麦秆锈病菌的至少一个物种引起的秆锈病的抗性。
本发明的方法在生产小麦植物中具有用途，其中所述小麦植物具有抵抗由小麦秆锈病菌的至少一个物种引起的秆锈病的增强的抗性。通常，与对照小麦植物抵抗小麦秆锈病菌的相同物种或多个物种的抗性相比，本发明的方法将题述小麦植物抵抗小麦秆锈病菌的至少一个物种的抗性增强或增加至少25％、50％、75％、100％、150％、200％、250％、500％或更高。除非另外陈述或者由使用内容显而易见，否则用于本发明的对照植物为不包含本发明的多核苷酸构建体的植物。优选地，对照植物与包含本发明的多核苷酸构建体的植物是基本一致的(例如相同的物种、亚种和变体)，不同之处在于所述对照不包含所述多核苷酸构建体。在一些实施方案中，所述对照包含多核苷酸构建体，但是不包含在本发明的多核苷酸构建中的一个或多个R基因序列。
此外，本发明提供了通过本发明的方法产生的和/或包含本发明的多核苷酸构建体的转化的植物、种子和植物细胞。此外，提供了包含本发明的多核苷酸构建体的后代植物及其种子。本发明还提供了通过本发明的转化的植物和/或后代植物产生的种子、蔬菜部分和其他植物部分以及由这种植物部分产生的、旨在被人和其他动物消耗或使用的食物产物和其他农业产物，所述其他动物包括但不限于宠物(例如狗和猫)和家畜(例如猪、奶牛、小鸡、火鸡和鸭子)。
本发明的组合物和方法的非限定性实例如下：
1.包含核苷酸序列的核酸分子，其中所述核苷酸序列选自：
(a)SEQ ID NO:1、4、7、10、19、22、25、34或49中列出的核苷酸序列；
(b)编码SEQ ID NO:2、5、8、11、20、23、26、35或50中列出的氨基酸序列的核苷酸序列，并且任选地，其中所述核苷酸序列不是天然形成的；
(c)SEQ ID NO:3、6、9、12、21、24、27、36或51中列出的核苷酸序列；
(d)与(a)或(c)中列出的核苷酸序列的至少一种具有至少85％序列一致性的核苷酸序列，其中所述核酸分子能够赋予包含所述核酸分子的小麦植物以抵抗秆锈病的抗性，并且任选地，其中所述核苷酸序列不是天然形成的；以及
(e)包含编码氨基酸序列的核苷酸序列的核酸分子，其中所述氨基酸序列与(c)中列出的至少一种氨基酸序列具有至少85％序列一致性，其中所述核酸分子能够赋予包含所述核酸分子的小麦植物以抵抗秆锈病的抗性，并且任选地，其中所述核苷酸序列不是天然形成的。
2.权利要求1的核酸分子，其中所述核酸分子为分离的核酸分子。
3.权利要求1或2的核酸分子，其中(d)或(e)的所述核酸分子编码包含卷曲螺旋结构域、核苷酸结合结构域和富含亮氨酸重复结构域的蛋白质。
4.权利要求3的核酸分子，其中所述卷曲螺旋结构域、所述核苷酸结合结构域和所述富含亮氨酸重复结构域的至少一种包含与SEQ ID NO:2或50中对应的结构域具有至少95％,96％,97％,98％,99％或100％的氨基酸序列。
5.权利要求3或4的核酸分子，其中所述富含亮氨酸重复结构域包含选自以下的氨基酸：在对应于SEQ ID NO:2的氨基酸607的氨基酸位置处的丝氨酸(S)，在对应于SEQ IDNO:2的氨基酸650的氨基酸位置处的酪氨酸(Y)，在对应于SEQ ID NO:2的氨基酸651的氨基酸位置处的丝氨酸(S)，以及在对应于SEQ ID NO:2的氨基酸657的氨基酸位置处的甘氨酸(G)。
6.表达盒，其包含权利要求1-5中任意一项的核酸分子以及可操作地连接的异源启动子。
7.载体，其包含权利要求1-5中任意一项的核酸分子或者权利要求6的表达盒。
8.权利要求7的载体，其进一步包含其他的小麦秆锈病抗性基因。
9.权利要求8的载体，其中所述其他的小麦秆锈病抗性基因选自Sr26、Sr32、Sr33、Sr39、Sr40、Sr47和Sr50。
10.使用权利要求1-5中任意一项的核酸分子、权利要求6的表达盒或权利要求7-9中任意一项的载体转化的宿主细胞。
11.权利要求10的宿主细胞，其中所述宿主细胞为植物细胞、细菌、真菌细胞或动物细胞。
12.权利要求10或11的宿主细胞，其中所述宿主细胞不是野生一粒小麦或栽培一粒小麦。
13.使用权利要求1-5中任意一项的核酸分子、权利要求6的表达盒或权利要求7-9中任意一项的载体转化的植物。
14.权利要求13的植物，其中所述植物为小麦植物。
15.权利要求14的植物，其中所述小麦植物不是野生一粒小麦或栽培一粒小麦。
16.包含在其基因组中稳定地引入多核苷酸构建体的转基因植物或种子，其中所述多核苷酸构建体包含选自以下的核苷酸序列：
(a)SEQ ID NO:1、4、7、10、19、22、25、34或49中列出的核苷酸序列；
(b)编码SEQ ID NO:2、5、8、11、20、23、26、35或50中列出的氨基酸序列的核苷酸序列，并且任选地，其中所述核苷酸序列不是天然形成的；
(c)SEQ ID NO:3、6、9、12、21、24、27、36或51中列出的核苷酸序列；
(d)与(a)或(c)中列出的核苷酸序列的至少一种具有至少85％序列一致性的核苷酸序列，其中所述核酸分子能够赋予包含所述核酸分子的小麦植物以抵抗秆锈病的抗性，并且任选地，其中所述核苷酸序列不是天然形成的；以及
(e)包含编码氨基酸序列的核苷酸序列的核酸分子，其中所述氨基酸序列与(c)中列出的至少一种氨基酸序列具有至少85％序列一致性，其中所述核酸分子能够赋予包含所述核酸分子的小麦植物以抵抗秆锈病的抗性，并且任选地，其中所述核苷酸序列不是天然形成的。
17.权利要求16的转基因植物或种子，其中(d)或(e)的所述核酸分子编码包含卷曲螺旋结构域、核苷酸结合结构域和富含亮氨酸重复结构域的蛋白质。
18.权利要求17的转基因植物或种子，其中所述卷曲螺旋结构域、所述核苷酸结合结构域和所述富含亮氨酸重复结构域的至少一种包含与SEQ IDNO:2或50中对应的结构域具有至少95％、96％、97％、98％、99％或100％的氨基酸序列。
19.权利要求17或18的转基因植物或种子，其中所述富含亮氨酸重复结构域包含选自以下的氨基酸：在对应于SEQ ID NO:2的氨基酸607的氨基酸位置处的丝氨酸(S)，在对应于SEQ ID NO:2的氨基酸650的氨基酸位置处的酪氨酸(Y)，在对应于SEQ ID NO:2的氨基酸651的氨基酸位置处的丝氨酸(S)，以及在对应于SEQ ID NO:2的氨基酸657的氨基酸位置处的甘氨酸(G)。
20.权利要求16-19中任意一项的转基因植物或种子，其中所述多核苷酸构建体包含(b)-(e)中任意一项的核苷酸序列并且进一步包含可操作地连接的用于在植物中表达所述核苷酸序列的启动子。
21.权利要求20的转基因植物或种子，其中所述启动子选自病原体诱导型、构成型、组织优选型、伤口诱导型和化学调节型启动子。
22.权利要求16-21中任意一项的转基因植物或种子，其中所述转基因植物为小麦植物并且所述转基因种子为小麦种子。
23.权利要求22的转基因植物或种子，其中相对于对照小麦植物而言，所述转基因植物或种子包含抵抗由小麦秆锈病菌的至少一个物种引起的小麦秆锈病的增强的抗性。
24.权利要求22或23的转基因植物或种子，其中所述多核苷酸构建体包含编码用于小麦秆锈病的R蛋白质的至少2个核苷酸序列。
25.权利要求24的转基因植物或种子，其中所述编码用于小麦秆锈病的R蛋白质的至少2个核苷酸序列的每一个编码用于小麦秆锈病的不同的R蛋白质。
26.用于增强小麦植物抵抗小麦秆锈病的抗性的方法，所述方法包括将多核苷酸构建体引入至少一种小麦植物细胞中，所述多核苷酸构建体包含选自以下的核苷酸序列：
(a)SEQ ID NO:1、4、7、10、19、22、25、34或49中列出的核苷酸序列；
(b)编码SEQ ID NO:2、5、8、11、20、23、26、35或50中列出的氨基酸序列的核苷酸序列，并且任选地，其中所述核苷酸序列不是天然形成的；
(c)SEQ ID NO:3、6、9、12、21、24、27、36或51中列出的核苷酸序列；
(d)与(a)或(c)中列出的核苷酸序列的至少一种具有至少85％序列一致性的核苷酸序列，其中所述核酸分子能够赋予包含所述核酸分子的小麦植物以抵抗秆锈病的抗性，并且任选地，其中所述核苷酸序列不是天然形成的；以及
(e)包含编码氨基酸序列的核苷酸序列的核酸分子，其中所述氨基酸序列与(c)中列出的至少一种氨基酸序列具有至少85％序列一致性，其中所述核酸分子能够赋予包含所述核酸分子的小麦植物以抵抗秆锈病的抗性，并且任选地，其中所述核苷酸序列不是天然形成的。
27.权利要求26的方法，其中(d)或(e)的所述核酸分子编码包含卷曲螺旋结构域、核苷酸结合结构域和富含亮氨酸重复结构域的蛋白质。
28.权利要求27的方法，其中所述卷曲螺旋结构域、所述核苷酸结合结构域和所述富含亮氨酸重复结构域的至少一种包含与SEQ ID NO:2或50中对应的结构域具有至少95％、96％、97％、98％、99％或100％的氨基酸序列。
29.权利要求27或28的方法，其中所述富含亮氨酸重复结构域包含选自以下的氨基酸：在对应于SEQ ID NO:2的氨基酸607的氨基酸位置处的丝氨酸(S)，在对应于SEQ IDNO:2的氨基酸650的氨基酸位置处的酪氨酸(Y)，在对应于SEQ ID NO:2的氨基酸651的氨基酸位置处的丝氨酸(S)，以及在对应于SEQ ID NO:2的氨基酸657的氨基酸位置处的甘氨酸(G)。
30.权利要求26-29中任意一项的方法，其中所述多核苷酸构建体被稳定地引入所述植物细胞的基因组中。
31.权利要求26-30中任意一项的方法，其中所述小麦植物细胞再生成在其基因组中包含所述多核苷酸构建体的小麦植物。
32.权利要求26-31中任意一项的方法，其中所述多核苷酸构建体包含(b)-(e)中任意一项的核苷酸序列并且进一步包含可操作地连接的用于在植物中表达所述核苷酸序列的启动子。
33.权利要求32的方法，其中所述启动子选自病原体诱导型、构成型、组织优选型、伤口诱导型和化学调节型启动子。
34.权利要求26-33中任意一项的方法，其中相对于对照小麦植物而言，包含所述多核苷酸构建体的所述小麦植物包含抵抗由小麦秆锈病菌的至少一个物种引起的小麦秆锈病的增强的抗性。
35.权利要求26-34中任意一项的方法，其中所述多核苷酸构建体包含编码小麦秆锈病的R蛋白质的至少2个核苷酸序列。
36.权利要求35的方法，其中所述编码用于小麦秆锈病的R蛋白质的所述至少2个核苷酸序列的每一个编码用于小麦秆锈病的不同的R蛋白质。
37.通过权利要求26-36中任意一项的方法产生的小麦植物。
38.权利要求37的小麦植物的种子，其中所述种子包含所述多核苷酸构建体。
39.在农业农作物生产中限制小麦秆锈病的方法，所述方法包括种植根据权利要求22-25和38中任意一项的小麦种子以及使小麦植物在有利于所述小麦植物生长和发育的条件下生长。
40.权利要求39的方法，其进一步包含由所述小麦植物收获至少一个种子。
41.权利要求22-25、37和38中任意一项的小麦植物或种子在农业中的用途。
42.使用权利要求22-25、37和38中任意一项的小麦植物或种子生产的人类或动物食物产品。
43.包含选自以下的氨基酸序列的多肽：
(a)由SEQ ID NO:1、4、7、10、19、22、25、34或49中列出的核苷酸序列编码的氨基酸序列；
(b)SEQ ID NO:2、5、8、11、20、23、26、35或50中列出的氨基酸序列；
(c)由SEQ ID NO:3中列出的核苷酸序列编码的氨基酸序列；以及
(d)与(c)中列出的至少一个氨基酸序列具有至少85％序列一致性的氨基酸序列，其中包含所述氨基酸序列的多肽能够赋予包含所述多肽的小麦植物以抵抗秆锈病的抗性，并且任选地，其中所述多肽不是天然形成的。
44.权利要求43的多肽，其中(d)的所述多肽包含卷曲螺旋结构域、核苷酸结合结构域和富含亮氨酸重复结构域。
45.权利要求44的多肽，其中所述卷曲螺旋结构域、所述核苷酸结合结构域和所述富含亮氨酸重复结构域的至少一种包含与SEQ ID NO:2或50中对应的结构域具有至少95％、96％、97％、98％、99％或100％的氨基酸序列。
46.权利要求44或45的多肽，其中所述富含亮氨酸重复结构域包含选自以下的氨基酸：在对应于SEQ ID NO:2的氨基酸607的氨基酸位置处的丝氨酸(S)，在对应于SEQ IDNO:2的氨基酸650的氨基酸位置处的酪氨酸(Y)，在对应于SEQ ID NO:2的氨基酸651的氨基酸位置处的丝氨酸(S)，以及在对应于SEQ ID NO:2的氨基酸657的氨基酸位置处的甘氨酸(G)。
47.用于鉴定小麦植物的方法，其中所述小麦植物展示新赋予的或增强的抵抗小麦秆锈病的抗性，所述方法包括检测所述小麦植物中选自Sr22和Sr45的R基因的存在情况。
48.权利要求47的方法，其中所述R基因的存在情况是通过检测所述R基因中至少一种标志物而检测的。
49.权利要求47或48的方法，其中所述方法包括检测Sr22和Sr45两者的存在情况。
50.权利要求47-49中任意一项的方法，其中所述Sr22包含SEQ ID NO:1、4、7、10、19、22、25或34中列出的所述核苷酸序列或由其组成，并且所述Sr45包含SEQ ID NO:49中列出的核苷酸序列或由其组成。
51.权利要求47-50中任意一项的方法，其中检测所述R基因的存在情况包括选自以下的一种：PCR扩增、核酸测序、核酸杂交以及用于检测R基因编码的R蛋白质的免疫测试。
52.通过权利要求47-51中任意一项的方法鉴定的小麦植物。
53.权利要求52的小麦植物的种子。
本发明的方法和组合物的其他实施方案在本发明的其他处描述。
本发明的方法用于增强小麦植物抵抗小麦秆锈病的抗性，特别是由小麦秆锈病菌的至少一个物种引起的秆锈病。本发明的优选的植物为小麦植物、小麦种子、小麦植物部分和小麦植物细胞。如本文所用，术语“小麦植物”通常是指作为小麦属的成员或者在小麦族(Triticeae tribe)内另一个属的成员的植物，特别是能够与至少一个小麦物种产生种间杂交物的另一个属的成员。这种小麦族内另一个属的实例为山羊草属和黑麦属。
本发明的小麦植物包括例如驯化的或非驯化的植物。本发明的小麦植物包括但不限于下述小麦属、山羊草属和黑麦属物种：普通小麦、栽培一粒小麦、圆锥小麦(T.turgidum)、野生一粒小麦、提莫菲维小麦(T.timopheevii)、乌拉尔图小麦(T.urartu)、粗山羊草(A.tauschii)、黑麦(S.cereale)及它们的杂交物。本发明中所包含的普通小麦亚种的实例是普通(普通小麦)、密穗(密穗小麦)、莫迦(莫迦小麦)、瓦维洛夫(瓦维洛夫小麦)、斯卑尔脱和印度圆粒小麦(sphaecrococcum，shot wheat)。本发明中所包含的圆锥小麦亚种的实例是圆锥(turgidum)、波斯(carthlicum)、二粒(dicoccom)、硬质(durum)、paleocoichicum、波兰(polonicum)、东方(turanicum)和野生二粒(dicoccoides)。本发明中所包含的栽培一粒小麦亚种的实例是一粒(单粒)和aegilopoide。在本发明的一个实施方案中，小麦植物为圆锥小麦物种的一员；具体为硬质小麦亚种的一员，例如Ciccio、Colosseo或Utopia栽培品种。应该认识到本发明的小麦植物可以是驯化的或非驯化的小麦植物。
本发明还涵盖了包含本发明的R基因的黑小麦植物、黑小麦植物部分和黑小麦植物细胞。如本文所用，“黑小麦植物”是指通过将黑麦植物(黑麦(Secale cereale))与四倍体小麦植物(例如圆锥小麦)或六倍体小麦植物(例如普通小麦)杂交而创建的植物。本发明还包括通过本发明所述黑小麦植物产生的种子，以及在本发明所述黑小麦植物附近控制杂草的方法。如本文所用，术语“小麦植物”涵盖了黑小麦植物，除非另外陈述或由使用内容显而易见。
术语“小麦植物”意图涵盖处于成熟或发育的任何阶段的小麦植物，以及由任何这种植物取得的或衍生的任何组织或器官(植物部分)，除非内容中另外清楚地说明。植物部分包括但不限于茎、根、花朵、胚珠、雄蕊、叶子、胚芽、分生区、愈伤组织、花药培养物、配子体、孢子体、花粉、小孢子、原生质体等。本发明还包括通过本发明的小麦植物产生的种子。
在本发明的一个实施方案中，编码R蛋白质的核苷酸序列与SEQ ID NO:1中列出的完整的核苷酸序列或其片段具有至少大约80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更高的序列一致性。在本发明的另一个实施方案中，编码R蛋白质的核苷酸序列与SEQ ID NO:3中列出的完整的核苷酸序列或其片段具有至少大约80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更高的序列一致性。
本发明涵盖分离的或基本纯化的多核苷酸(在本发明中还称为“核酸分子”、“核酸”等)或蛋白质(在本发明中还称为“多肽”)组合物，其包含例如在所附的序列表中列出的序列的多核苷酸和蛋白质以及这种多核苷酸和蛋白质的变体和片段。“分离的”或“纯化的”多核苷酸或蛋白质或者其生物活性部分基本上或本质上不含在其天然形成环境中发现的正常情况下与所述多核苷酸或蛋白质伴随或相互作用的成分。因此，当通过重组技术生产时，分离的或纯化的多核苷酸或蛋白质基本上不含其他的细胞物质或培养介质，或者当化学合成时，基本上不含化学前体或其他化学物质。最佳地，在衍生多核苷酸的有机体的基因组DNA中，“分离的”多核苷酸不含天然情况下位于所述多核苷酸的侧翼(即，位于所述多核苷酸的5'和3'末端的序列)的序列(最佳地，蛋白质编码序列)。例如在多个实施方案中，分离的多核苷酸可以包含少于大约5kb、4kb、3kb、2kb、1kb、0.5kb或0.1kb的核苷酸序列，其中所述核苷酸序列天然情况下位于衍生所述多核苷酸的细胞的基因组DNA中的多核苷酸的侧翼。基本上不含细胞物质的蛋白质包括蛋白质的制备物，其具有少于大约30％、20％、10％、5％或1％(以干重计)的污染蛋白质。当本发明的蛋白质或其生物活性部分以重组方式生产时，最佳的培养介质表示少于大约30％、20％、10％、5％或1％(以干重计)的化学前体或目的非蛋白质化学物质。
本发明公开的多核苷酸及由其编码的蛋白质的片段和变体也涵盖在本发明的范围内。“片段”是指多核苷酸的部分，或氨基酸序列及因此由其编码的蛋白质的部分。包含编码序列的多核苷酸的片段可以编码保留全长或天然蛋白质的生物活性的蛋白质片段。备选地，用作杂交探针的多核苷酸的片段通常不编码保留生物活性或不保留启动子活性的蛋白质。因此，核苷酸序列的片段可以为至少大约20个核苷酸、大约50个核苷酸、大约100个核苷酸以及多至本发明的全长的多核苷酸。
作为天然R多核苷酸的片段的多核苷酸包含至少16、20、50、75、100、125、150、175、200、300、400、500、1000、2000、5000、7500或9500个相邻的核苷酸，或者多至本发明公开的全长R多核苷酸中存在的核苷酸的数量(例如SEQ ID NO:1中9859个核苷酸)。
“变体”是指基本相似的序列。就多核苷酸而言，变体包括具有以下的多核苷酸：在5'和/或3'末端的删除(即，截短)；在天然多核苷酸中的一个或多个内部位点处一个或多个核苷酸的删除和/或加入；和/或在天然多核苷酸中的一个或多个位点处的一个或多个核苷酸的取代。如本文所用，“天然的”多核苷酸或多肽分别包括天然形成的核苷酸序列或氨基酸序列。就多核苷酸而言，保守的变体包括由于遗传密码的简并性而编码本发明的一种R蛋白质的氨基酸序列的那些序列。天然形成的等位基因变体例如这些可以使用公知的分子生物学技术鉴定，例如下文概述的聚合酶链式反应(PCR)和杂交技术。变体多核苷酸还包括合成衍生的多核苷酸，例如通过使用定点诱变生成但是仍然编码本发明的R蛋白质的那些。通常，本发明的特定的多核苷酸的变体与特定的多核苷酸具有至少大约80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更高的序列一致性，这是通过本发明其他处所述的序列比对程序和参数测定。在本发明的某些实施方案中，本发明的特定的多核苷酸变体与选自SEQ ID NO:1、3、4、6、7、9、10、12、19、21、22、24、25、27、34、36、49和51的至少一个核苷酸序列具有至少大约80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更高的序列一致性，并且任选地，包含与SEQ ID NO:1、3、4、6、7、9、10、12、19、21、22、24、25、27、34、36、49和/或51中列出的核苷酸序列具有至少一个核苷酸修饰的不同的非天然形成的核苷酸序列，其中所述核苷酸修饰选自至少一个核苷酸的取代、至少一个核苷酸的加入和至少一个核苷酸的删除。
还可以通过比较变体多核苷酸编码的多肽与参照多核苷酸编码的多肽之间的百分序列一致性来评价本发明的特定的多核苷酸(即，参照多核苷酸)的变体。因此，公开了例如编码多肽的多核苷酸，其中所述多肽与SEQ ID NO:2、5、8、11、20、23、26、35和50的至少一个的多肽具有给定的百分序列一致性。可以使用本发明其他处描述的序列比对程序和参数来计算任2个多肽之间的百分序列一致性。在通过比较本发明的任意给定的一对多核苷酸所编码的2个多肽所共有的百分序列一致性来评价所述这对多核苷酸的情况下，2个编码的多肽之间的百分序列一致性为至少大约60％、65％、70％、75％、80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更高的序列一致性。在本发明的某些实施方案中，本发明的特定的多肽的变体与选自SEQ ID NO:2、5、8、11、20、23、26、35和50中的至少一个氨基酸序列具有至少大约80％、85％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更高的序列一致性，并且任选地，包含与SEQ IDNO:2、5、8、11、20、23、26、35和/或50中列出的氨基酸序列具有至少一个氨基酸修饰的不同的非天然形成的氨基酸序列，其中所述氨基酸修饰选自至少一个氨基酸的取代、至少一个氨基酸的加入和至少一个氨基酸的删除。
“变体”蛋白质是指通过以下方式由天然蛋白质衍生的蛋白质：在天然蛋白质的N-末端和/或C-末端处的一个或多个氨基酸的删除(所谓的截短)；在天然蛋白质的一个或多个内部位点处的一个或多个氨基酸的删除和/或加入；或者在天然蛋白质的一个或多个位点处的一个或多个氨基酸的取代。这种变体可以得自例如遗传多态性或得自人类的操作。R蛋白质的生物活性变体与天然蛋白质的氨基酸序列(例如SEQ ID NO:2、5、8、11、20、23、26、35和/或50中列出的氨基酸序列)具有至少大约80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更高的序列一致性，这是通过在本发明中其他处所述序列比对程序和参数测定。本发明的蛋白质的生物活性变体与所述蛋白质可以具有1-15个氨基酸残基、1-10个(例如6-10)、5个、4、3、2或者甚至1个氨基酸残基的不同。
本发明的蛋白质可以以多种方式改变，包括氨基酸取代、删除、截短和插入。用于这种操作的方法是本领域公知的。用于诱变和多核苷酸改变的方法是本领域公知的。例如参见Kunkel(1985)Proc.Natl.Acad.Sci.USA 82:488-492；Kunkel et al.(1987)Methodsin Enzymol.154:367-382；U.S.Patent No.4,873,192；Walker and Gaastra,eds.(1983)Techniques in Molecular Biology(MacMillan Publishing Company,New York)和其中引用的参考文献。不影响目的蛋白质的生物活性的合适的氨基酸取代的指导可以在Dayhoff et al.(1978)Atlas of Protein Sequence and Structure(Natl.Biomed.Res.Found.,Washington,D.C.)的模型中找到，该文献以引用方式并入本文。保守性取代(例如将一个氨基酸与具有相似性质的另一个氨基酸交换)可以是最佳的。
因此，本发明的基因和多核苷酸包括天然形成的序列以及突变体和其他变体形式。类似地，本发明的蛋白质涵盖天然形成的蛋白质以及其改变和修饰形式。更优选地，这种变体赋予包含该变体的植物或其部分以抵抗由小麦秆锈病菌的至少一个物种引起的小麦秆锈病的增强的抗性。在一些实施方案中，在编码变体的DNA中形成的突变不会将所述序列置于阅读框外。最佳地，突变不会创建会产生二级mRNA结构的互补区。参见EP专利申请公开号75,444。
预计本发明涵盖的蛋白质序列的删除、插入和取代在蛋白质的特征中不会产生自由基变化。但是，在进行取代、删除或插入之前难以预计此类操作的确切作用时，本领域的技术人员将理解所述作用通过常规的筛选测定法来评价。换言之，可以通过下文公开的测定法来评价活性。
例如可以使用Sr22或Sr45多核苷酸转化小麦植物，该小麦植物对于由小麦秆锈病菌的特定物种引起的小麦秆锈病是易感的，然后所述小麦植物再生成包含所述多核苷酸的转化的或转基因的植物，并使用本领域已知的或本发明中其他处所述标准的抗性测定法，针对由小麦秆锈病菌的特定物种引起的小麦秆锈病的抗性进行测试。本发明的优选的变体多核苷酸或多肽赋予或者能够赋予小麦植物以抵抗小麦秆锈病菌的至少一个物种的增强的抗性，其中已知所述小麦秆锈病菌在易感小麦植物中会引起小麦秆锈病。
变体多核苷酸和蛋白质还涵盖由诱变和重组过程(例如DNA改组)衍生的序列和蛋白质。这种DNA改组的策越是本领域已知的。例如参见Stemmer(1994)Proc.Natl.Acad.Sci.USA 91:10747-10751；Stemmer(1994)Nature 370:389-391；Crameriet al.(1997)Nature Biotech.15:436-438；Moore et al.(1997)J.Mol.Biol.272:336-347；Zhang et al.(1997)Proc.Natl.Acad.Sci.USA 94:4504-4509；Crameri et al.(1998)Nature 391:288-291；以及美国专利号5,605,793和5,837,458。
本发明的多核苷酸可以用于从其他有机体，特别是其他植物，分离相应的序列。在这种方式中，诸如PCR、杂交等之类的方法可以用于基于它们的序列与本发明列出的序列的同源性来鉴定这种序列。基于它们的序列与本发明列出的完整序列或者其变体和片段的序列一致性而分离的序列被涵盖在本发明之内。这种序列包括作为本发明公开的序列的直系同源物的序列。“直系同源物”是指由共同的祖先基因衍生并且作为物种形成的结果在不同物种中发现的基因。当在不同的物种中发现的基因的核苷酸序列和/或它们编码的蛋白质序列共享至少60％、70％、75％、80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更高的序列一致性时，任何这些基因是直系同源物。在多种物种中，直系同源物的功能通常是高度保守的。因此，编码R蛋白质并且在严谨条件下与本发明公开的或者本领域已知的至少一种R蛋白质杂交的分离的多核苷酸、或者它们的变体或片段被涵盖在本发明之内。
在一个实施方案中，本发明的直系同源物具有编码序列，其与选自SEQ ID NO:1、3、4、6、7、9、10、12、19、21、22、24、25、27、34、36、49和51中列出的核苷酸序列的核苷酸序列包含至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更高的核苷酸序列一致性，和/或本发明的直系同源物编码蛋白质，其与选自SEQ ID NO:2、5、8、11、20、23、26、35和50中列出的氨基酸序列的氨基酸序列包含至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或更高的氨基酸序列一致性。
如图8和9所示，SR22和SR45蛋白质包含某些保守的结构域。在得自Schomburgk(包含SEQ ID NO:2中列出的氨基酸序列)的SR22中，保守的结构域包含例如卷曲螺旋结构域(氨基酸120-147)、核苷酸结合结构域(氨基酸178-465)和富含亮氨酸重复结构域(氨基酸601-853)。在SR45(包含SEQ ID NO:50中列出的氨基酸序列)中，保守的结构域包含例如卷曲螺旋结构域(氨基酸32-55)、核苷酸结合结构域(氨基酸175-452)和富含亮氨酸重复结构域(氨基酸578-867)。优选地，本发明的变体SR22和SR45蛋白质包含对应于分别在图8和9中示出的结构域的卷曲螺旋结构域、核苷酸结合结构域和富含亮氨酸重复结构域。
在一些实施方案中，与本发明公开的SR22(例如SEQ ID NO:2、5、8、11、20、23、26或35)或SR45(例如SEQ ID NO:50)蛋白质的一个或多个全长氨基酸序列相比，本发明的变体SR22和SR45蛋白质包含与1个、2个或3个这种保守结构域的更高百分率的氨基酸序列一致性。优选地，这种变体包含相应的结构域或多个结构域，所述结构域与图8所示的1个、2个或3个保守的结构域具有至少95％、96％、97％、98％、99％或100％序列一致性，并且所述变体进一步包含氨基酸序列，该序列与选自SEQ ID NOS:2、5、8、11、20、23、26、35和50中列出的氨基酸序列的氨基酸序列具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％一致性。
在一些实施方案中，本发明的变体SR22蛋白质包含SEQ ID NO:2的氨基酸序列的以下氨基酸中的至少一种，其中所述氨基酸在SR22蛋白质(包含SEQ ID NO:2、5、8、11、20、23、26和35中列出的氨基酸序列)的富含亮氨酸重复结构域中是保守的：在氨基酸位置607处的丝氨酸(S)，在氨基酸位置650处的酪氨酸(Y)，在氨基酸位置651处的丝氨酸(S)，以及在氨基酸位置657处的甘氨酸(G)。在优选的实施方案中，变体SR22蛋白质包含1个、2个、3个或4个这些保守的氨基酸，并且任选地包含与选自SEQ ID NOS:2、5、8、11、20、23、26、35和50中列出的氨基酸序列的氨基酸序列具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％一致性的氨基酸序列，并且备选地包含或者任选地进一步包含与分别在图8中所示的1个、2个或3个保守的结构域具有至少95％、96％、97％、98％、99％或100％序列一致性的相应的结构域或多个结构域。
应该认识到变体SR22和SR45蛋白质中的结构域(对应于图8和9中所示的那些保守的结构域)以及其中任何特定的保守的氨基酸可以通过本领域那些技术人员已知的或者本发明其他处公开的方法鉴定，例如多重序列比对。进一步认识到在特定的变体SR22和SR45中的这种保守的结构域和保守的氨基酸的位置可以由SEQ ID NO:2和50中列出的氨基酸序列中的位置处改变，并且通过诸如多重序列比对之类的方法，针对本发明的任何变体SR22或SR45蛋白质，可以测定这种保守的结构域和保守的氨基酸的相应位置。
优选地，本发明的变体SR22或SR45蛋白质以及编码它们的多核苷酸赋予或者能够赋予包含这种蛋白质或多核苷酸的小麦植物以抵抗小麦秆锈病菌的至少一个物种的增强的抗性，其中已知所述小麦秆锈病菌在易感小麦植物中引起小麦秆锈病。
在PCR方法中，可以将寡核苷酸引物设计用于PCR反应中，以从由任何目的植物提取的cDNA或基因组DNA扩增相应的DNA序列。用于设计PCR引物和PCR克隆的方法通常是本领域已知的，并且在Sambrook et al.(1989)Molecular Cloning:A Laboratory Manual(2ded.,Cold Spring Harbor Laboratory Press,Plainview,New York)中公开。还参见Inniset al.,eds.(1990)PCR Protocols:A Guide to Methods and Applications(AcademicPress,New York)；Innis and Gelfand,eds.(1995)PCR Strategies(Academic Press,NewYork)和Innis and Gelfand,eds.(1999)PCR Methods Manual(Academic Press,NewYork)。已知的PCR方法包括但不限于使用成对引物、套式引物、单一特异性引物、简并引物、基因特异性引物、载体特异性引物、部分错配引物等的方法。
在杂交技术中，使用已知多核苷酸的全部或部分作为探针，该探针与由所选有机体得到的克隆基因组DNA片段或cDNA片段的群体(即，基因组或cDNA文库)中存在的其他相应的多核苷酸选择性地杂交。杂交探针可以为基因组DNA片段、cDNA片段、RNA片段或其他寡核苷酸，并且可以使用可检测基团(例如32P)或任何其他的可检测标志物进行标记。因此，例如用于杂交的探针可以通过基于本发明的多核苷酸的加标记的合成寡核苷酸制成。用于制备杂交用探针以及用于cDNA和基因组文库构建的方法通常是本领域已知的，并且在Sambrook et al.(1989)Molecular Cloning:A Laboratory Manual(2d ed.,Cold SpringHarbor Laboratory Press,Plainview,New York)中公开。
例如本发明公开的完整多核苷酸或者其一个或多个部分可以用作能够与相应的多核苷酸和信使RNA特异性杂交的探针。为了在多种条件下取得特异性杂交，这种探针包含在基因序列或者目的序列的cDNA中是独特的序列，并且最佳地，长度为至少大约10个核苷酸，最佳地长度为至少大约20个核苷酸。这种探针可以用于通过PCR扩增用于由所选植物得到的特定目的基因的相应多核苷酸。这种技术可以用于由所需的植物分离其他的编码序列，或者作为诊断测定法来确定植物中编码序列的存在情况。杂交技术包括铺板的DNA文库的杂交筛选(噬菌斑或集落，例如参见Sambrook et al.(1989)Molecular Cloning:ALaboratory Manual(2d ed.,Cold Spring Harbor Laboratory Press,Plainview,NewYork))。
这种序列的杂交可以在严谨条件下实施。“严谨条件”或“严谨的杂交条件”是指在其中探针将以比与其他序列更高的可检测程度(例如超过背景至少2倍)与其靶序列杂交的条件。严谨条件是序列依赖的，并且在不同的环境中不同。通过控制杂交的严谨性和/或洗涤条件，可以鉴定与探针具有100％互补性的靶序列(同源探针)。备选地，可以调节严谨条件，从而允许序列中具有一些错配，使得检测更低程度的相似性(异源探针)。通常，探针的长度少于大约1000个核苷酸，最佳地，长度少于500个核苷酸。
通常，严谨条件为其中在pH 7.0至8.3的盐浓度低于大约1.5M Na离子，通常为大约0.01至1.0M Na离子浓度(或其他盐)，并且温度为至少大约30℃(对于短探针，例如10至50个核苷酸)和至少大约60℃(对于长探针，例如超过50个核苷酸)的那些条件。严谨条件还可以通过加入去稳定试剂(例如甲酰胺)取得。示例性的低严谨条件包括在37℃使用30至35％甲酰胺、1MNaCl、1％SDS(十二烷基硫酸钠)的缓冲溶液的杂交，以及在50℃至55℃在1x至2x SSC(20X SSC＝3.0M NaCl/0.3M柠檬酸三钠)中洗涤。示例性的中度严谨条件包括在37℃在40至45％甲酰胺、1.0M NaCl、1％SDS中杂交，以及在55℃至60℃在0.5x至1x SSC中洗涤。示例性的高严谨条件包括在37℃在50％甲酰胺、1M NaCl、1％SDS中杂交，以及在60℃至65℃在0.1X SSC中洗涤。任选地，洗涤缓冲剂可以包含大约0.1％至大约1％SDS。杂交的持续时间通常少于大约24小时，通常为大约4至大约12小时。洗涤时间的持续时间为至少足以达到平衡的时间长度。
特异性通常为杂交后洗涤的函数，重要的因子是最终洗涤溶液的离子强度和温度。对于DNA-DNA杂交物而言，Tm可以由Meinkoth and Wahl(1984)Anal.Biochem.138:267-284的等式近似得到：Tm＝81.5℃+16.6(log M)+0.41(％GC)-0.61(％form)-500/L；其中M为一价阳离子的摩尔浓度，％GC为DNA中鸟嘌呤和胞嘧啶核苷酸的百分率，％form为杂交溶液中甲酰胺的百分率，而L为碱基对中杂交物的长度。Tm是50％互补性靶序列与完美匹配的探针杂交的温度(在所定义的离子强度和pH下)。每错配1％，Tm降低大约1℃；因此可以调节Tm、杂交和/或洗涤条件，从而与所需一致性的序列杂交。例如如果寻求一致性>90％的序列，则Tm可以降低10℃。通常，在所定义的离子强度和pH下，对于特定的序列及其互补物，将严谨条件选择为低于热熔点(Tm)大约5℃。然而，严格的严谨条件可以在低于热熔点(Tm)1、2、3或4℃采用杂交和/或洗涤；中度严谨条件可以在低于热熔点(Tm)6、7、8、9或10℃采用杂交和/或洗涤；低严谨条件可以在低于热熔点(Tm)11、12、13、14、15或20℃采用杂交和/或洗涤。利用等式、杂交和洗涤组合物以及所需的Tm，那些普通技术人员将理解的是杂交严谨性和/或洗涤溶液的改变本身就有所描述。如果所需的错配程度使得Tm低于45℃(水性溶液)或32℃(甲酰胺溶液)，最佳的是增加SSC浓度，从而可以使用更高的温度。对核酸杂交的广泛的指导在Tijssen(1993)Laboratory Techniques in Biochemistry and MolecularBiology—Hybridization with Nucleic Acid Probes,Part I,Chapter 2(Elsevier,NewYork)和Ausubel et al.,eds.(1995)Current Protocols in Molecular Biology,Chapter 2(Greene Publishing and Wiley-Interscience,New York)中找到。参见Sambrook et al.(1989)Molecular Cloning:A Laboratory Manual(2d ed.,Cold SpringHarbor Laboratory Press,Plainview,New York)。
应该认识到本发明的R蛋白质编码序列涵盖包含与SEQ ID NO:1、3、4、6、7、9、10、12、19、21、22、24、25、27、36、37、39、40、42、49和51的任意一个或多个的核苷酸序列是足够一致的核苷酸序列的多核苷酸分子。术语“足够一致的”在本发明中用于指第一氨基酸或核苷酸序列，其含有与第二氨基酸或核苷酸序列足够或最少数量的一致的或相当的(例如具有相似的侧链)氨基酸残基或核苷酸，使得第一和第二氨基酸或核苷酸序列具有共同的结构结构域和/或共同的功能活性。例如包含共同的结构结构域(具有至少大约45％、55％或65％一致性，优选为75％一致性，更优选为85％、86％、87％、88％、89％、90％、95％、96％、97％、98％或99％一致性)的氨基酸或核苷酸序列在本发明中定义为足够一致的。
为了确定2个氨基酸序列或2个核酸的百分一致性，针对最佳比较对序列进行比对。2个序列之间的百分一致性为序列所共有的相同位置的数量的函数(即，百分一致性＝相同位置的数量/位置的总数量(例如重叠的位置)x 100)。在一个实施方案中，2个序列为相同的长度。2个序列之间的百分一致性可以使用类似于下文所述那些的技术来确定(允许或不允许空位)。在计算百分一致性中，通常计数确切的匹配。
2个序列之间的百分一致性的确定可以使用数学算法来完成。用于比较2个序列的数学算法的优选的非限定性实例为Karlin and Altschul(1990)Proc.Natl.Acad.Sci.USA87:2264，在Karlin and Altschul(1993)Proc.Natl.Acad.Sci.USA 90:5873-5877)中修改的算法。这种算法被引入至Altschul et al.(1990)J.Mol.Biol.215:403的NBLAST和XBLAST程序中。可以使用NBLAST程序(得分＝100，字长＝12)进行BLAST核苷酸检索，从而得到与本发明的多核苷酸分子同源的核苷酸序列。可以使用XBLAST程序(得分＝50，字长＝3)进行BLAST蛋白质检索，从而得到与本发明的蛋白质分子同源的氨基酸序列。为了获得用于比较目的的空位的比对，可以使用Gapped BLAST，如Altschul et al.(1997)NucleicAcids Res.25:3389中所述。备选地，PSI-Blast可以用于进行检测分子之间具有较远关系的迭代检索。参见Altschul et al.(1997)如上文。当使用BLAST、Gapped BLAST和PSI-Blast程序时，各程序的缺省参数(例如可利用互联网ncbi.nlm.nih.gov上的XBLAST和NBLAST)。用于序列比较的数学算法的另一个优选的非限定性实例为Myers and Miller(1988)CABIOS 4:11-17的算法。这种算法被引入至ALIGN程序(2.0版)中，其为GCG序列比对软件包的部分。当使用ALIGN程序用于比较氨基酸序列时，可以使用PAM120加权残基表(weight residue table)、空位长度罚分12和空位罚分4。还可以通过检查进行人工比对。
除非另外陈述，否则本发明提供的序列一致性/相似性值是指使用本发明的全长序列，并通过算法Clustal W(Nucleic Acid Research,22(22):4673-4680,1994)使用软件包Vector NTI Suite Version 7(InforMax,Inc.,Bethesda,MD,USA)中包含的程序AlignX使用缺省参数；或者其任何相当的程序而使用多重比对而获得的值。“相当的程序”是指任何序列比较程序，对于任意2个所考虑的序列而言，所述程序生成这样的比对，与通过CLUSTALW(Version 1.83)使用缺省参数(可利用the European BioinformaticsInstitute website的互联网：ebi.ac.uk/Tools/clustalw/index.html)生成的相应的比对相比时，所述比对具有一致的核苷酸或氨基酸残基匹配以及一致的百分序列一致性。
术语“多核苷酸”的使用不旨在将本发明限制在包含DNA的多核苷酸。本领域的那些普通技术人员将认识到多核苷酸可以包含核糖核苷酸，以及核糖核苷酸和脱氧核糖核苷酸的组合。这种脱氧核糖核苷酸和核糖核苷酸包括天然形成的分子和合成的类似物。本发明的多核苷酸还涵盖所有形式的序列，包括但不限于单链形式、双链形式、发夹、茎环结构等。
包含R蛋白质编码区的多核苷酸构建体可以在用于在目的植物或其他有机体或非人类宿主细胞中表达的表达盒中提供。所述表达盒包含与R蛋白质编码区可操作地连接的5'和3'调控序列。“可操作地连接”是指2个或更多个元件之间的功能连接。例如目的多核苷酸或基因与调控序列(即，启动子)之间的可操作地连接是允许目的多核苷酸表达的功能连接。可操作地连接的元件可以是相邻的或非相邻的。当可操作地连接用于指2个蛋白质编码区的连接时，可操作地连接是指编码区处于相同的阅读框中。所述表达盒可以另外包含有待共同转化至有机体中的至少一个其他的基因。备选地，其他的基因可以提供在多重表达盒上。这种表达盒与用于插入R蛋白质编码区在调控区的转录调控下的多个限制性位点和/或重组位点一起提供。表达盒可以另外包含选择性标志物基因。
表达盒沿着转录的5'-3'方向包含转录和翻译起始区(即，启动子)、本发明的R蛋白质编码区以及在植物或其他有机体或非人类宿主细胞中具有功能的转录和翻译终止区(即，终止区)。调控区(即，启动子、转录调控区和翻译终止区)和/或本发明的R蛋白质编码区对于宿主细胞或彼此而言可以是天然的/类似的。备选地，调控区和/或本发明的R蛋白质编码区对于宿主细胞或彼此而言可以是异源的。
如本文所用，关于核酸分子或核苷酸序列，“异源的”是起源于外来物种的核酸分子或核苷酸序列，或者如果起源于相同的物种，则通过谨慎的人类干预在组合物和/或基因组基因座中由其天然形式修饰得到。例如与异源多核苷酸可操作地连接的启动子得自一种物种，该物种不同于衍生所述多核苷酸的物种，或者如果得自相同/类似的物种，则一个或两个基本由它们的起源形式和/或基因组基因座修饰得到，或者启动子不是用于可操作地连接的多核苷酸的天然启动子。如本文所用，嵌合基因包含与转录起始区可操作地连接的编码序列，其中所述转录起始区对于编码序列而言是异源的。
本发明提供了至少包含本发明的核酸分子、表达盒和载体的宿主细胞。在本发明的优选的实施方案中，宿主细胞为植物细胞。在其他的实施方案中，宿主细胞选自细菌、真菌细胞和动物细胞。在某些实施方案中，宿主细胞为非人类动物细胞。然而，在一些其他的实施方案中，宿主细胞为体外培养的人类细胞。
尽管使用异源启动子表达R蛋白质可能是最佳的，但是可以使用相应的R基因的天然启动子。
终止区对于转录起始区可以是天然的，与目的可操作地连接的R蛋白质编码区是天然的，与植物宿主可以是天然的，或者可以由另一个来源衍生(即，对于启动子、目的R蛋白质和/或植物宿主而言是外来的或异源的)，或它们的组合。常规的终止区可以得自根癌土壤杆菌(A.tumefaciens)的Ti质粒，例如章鱼碱合成酶和蓝曙红合成酶终止区。还参见Guerineau et al.(1991)Mol.Gen.Genet.262:141-144；Proudfoot(1991)Cell 64:671-674；Sanfacon et al.(1991)Genes Dev.5:141-149；Mogen et al.(1990)Plant Cell 2:1261-1272；Munroe et al.(1990)Gene 91:151-158；Ballas et al.(1989)Nucleic AcidsRes.17:7891-7903；和Joshi et al.(1987)Nucleic Acids Res.15:9627-9639。
在合适的情况下，可以针对在转化植物中增加的表达，优化所述多核苷酸。换言之，可以使用用于改进表达的植物优选密码子合成所述多核苷酸。例如参见Campbell andGowri(1990)Plant Physiol.92:1-11for a discussion of host-preferred codonusage。本领域中可利用多种方法用于合成植物优选的基因。例如参见美国专利号5,380,831和5,436,391，以及Murray et al.(1989)Nucleic Acids Res.17:477-498，这些文献以引用方式并入本文。
已知其他的序列修饰用于增加细胞宿主中的基因表达。这些包括删除编码伪多腺苷酸信号的序列、外显子-内含子剪接位点信号、转座子样重复序列和可能有损于基因表达的其他此类良好表征的序列。可以将序列的G-C含量调节至给定细胞宿主的平均水平，其是通过参照在宿主细胞中表达的已知基因来计算的。在可能的情况下，对序列进行修饰，从而避免预计的发夹二级mRNA结构。
所述表达盒可以另外包含5'前导序列。这种前导序列可以发挥增强翻译的作用。翻译前导序列是本领域已知的，并且包括：小核糖核酸病毒前导序列，例如EMCV前导序列(脑心肌炎5'非编码区)(Elroy-Stein et al.(1989)Proc.Natl.Acad.Sci.USA 86:6126-6130)；马铃薯Y病毒属前导序列，例如TEV前导序列(烟草蚀纹病毒)(Gallie et al.(1995)Gene 165(2):233-238)，MDMV前导序列(玉米矮花叶病毒)(Virology 154:9-20)，和人类免疫球蛋白重链结合蛋白质(BiP)(Macejak et al.(1991)Nature 353:90-94)；由苜蓿花叶病毒的衣壳蛋白质mRNA得到的非翻译前导序列(AMV RNA4)(Jobling et al.(1987)Nature325:622-625)；烟草花叶病毒前导序列(TMV)(Gallie et al.(1989)in MolecularBiology of RNA,ed.Cech(Liss,New York),pp.237-256)；以及玉米萎黄病毒前导序列(MCMV)(Lommel et al.(1991)Virology 81:382-385)。还参见Della-Cioppa et al.(1987)Plant Physiol.84:965-968。
在制备所述表达盒中，可以对多个DNA片段进行操作，从而以合适的取向并且如何合适，在合适的阅读框中提供DNA序列。为此，可以使用接头或连接体来连接DNA片段，或者可以涉及其他的操作从而提供常规的限制性位点、除去多余的DNA、除去限制性位点等。就此目的而言，可以涉及体外诱变、引物修复、限制、退火、再替代(resubstitution)，例如转换和颠换。
多种启动子可以用于本发明的实践中。可以根据所需的结果来选择启动子。核酸可以与构成型、组织优选型或用于植物中表达的其他启动子结合。这种构成型启动子包括例如核心CaMV 35S启动子(Odell et al.(1985)Nature 313:810-812)；水稻肌动蛋白(McElroy et al.(1990)Plant Cell 2:163-171)；泛素(Christensen et al.(1989)PlantMol.Biol.12:619-632and Christensen et al.(1992)Plant Mol.Biol.18:675-689)；pEMU(Last et al.(1991)Theor.Appl.Genet.81:581-588)；MAS(Velten et al.(1984)EMBO J.3:2723-2730)；ALS启动子(美国专利号5,659,026)等。其他的构成型启动子包括例如美国专利号5,608,149；5,608,144；5,604,121；5,569,597；5,466,785；5,399,680；5,268,463；5,608,142；和6,177,611。
组织优选型启动子可以用于靶向特定植物组织中R蛋白质编码序列的增强的表达。这种组织优选型启动子包括但不限于叶子优选型启动子、根优选型启动子、种子优选型启动子和茎优选型启动子。组织优选型启动子包括Yamamoto et al.(1997)Plant J.12(2):255-265；Kawamata et al.(1997)Plant Cell Physiol.38(7):792-803；Hansen etal.(1997)Mol.Gen Genet.254(3):337-343；Russell et al.(1997)Transgenic Res.6(2):157-168；Rinehart et al.(1996)Plant Physiol.112(3):1331-1341；Van Camp etal.(1996)Plant Physiol.112(2):525-535；Canevascini et al.(1996)PlantPhysiol.112(2):513-524；Yamamoto et al.(1994)Plant Cell Physiol.35(5):773-778；Lam(1994)Results Probl.Cell Differ.20:181-196；Orozco et al.(1993)Plant MolBiol.23(6):1129-1138；Matsuoka et al.(1993)Proc Natl.Acad.Sci.USA 90(20):9586-9590；和Guevara-Garcia et al.(1993)Plant J.4(3):495-505。对于弱表达而言，如果需要，可以修饰这种启动子。
通常，有利的是由诱导型启动子表达基因，特别是由病原体诱导型启动子。这种启动子包括由致病相关蛋白质(PR蛋白质)得到的那些，其在由病原体感染后诱导；例如PR蛋白质、SAR蛋白质、β-1,3-葡聚糖酶、几丁质酶等。例如参见Redolfi et al.(1983)Neth.J.Plant Pathol.89:245-254；Uknes et al.(1992)Plant Cell 4:645-656；和VanLoon(1985)Plant Mol.Virol.4:111-116。还参见WO 99/43819，这些文献以引用方式并入本文。
关注在病原体感染位点处或附近局部表达的启动子。例如参见Marineau et al.(1987)Plant Mol.Biol.9:335-342；Matton et al.(1989)Molecular Plant-MicrobeInteractions 2:325-331；Somsisch et al.(1986)Proc.Natl.Acad.Sci.USA 83:2427-2430；Somsisch et al.(1988)Mol.Gen.Genet.2:93-98；和Yang(1996)Proc.Natl.Acad.Sci.USA 93:14972-14977。也参见Chen et al.(1996)Plant J.10:955-966；Zhang et al.(1994)Proc.Natl.Acad.Sci.USA 91:2507-2511；Warner et al.(1993)Plant J.3:191-201；Siebertz et al.(1989)Plant Cell 1:961-968；美国专利号5,750,386(线虫诱导型)；并且这些文献以引用方式并入本文。特别关注用于玉米PRm基因的诱导型启动子，所述基因的表达是由病原体串珠镰刀菌(Fusarium moniliforme)诱导的(例如参见Cordero et al.(1992)Physiol.Mol.Plant Path.41:189-200)。
也关注由靶物种得到的其他抗性基因的天然启动子。这些启动子通常是病原体诱导型的，并且可能以合适的水平并且在合适的组织中表达抗性基因。这种启动子的实例为小麦的Sr57/Lr34、Sr33和Sr35启动子(Risk et al.(2012)Plant Biotechnol J 10:447-487；Periyannan et al.(2013)Science 341:786-788；Saintenac et al.(2013)Science341:783-786)。
此外，当病原体通过伤口或昆虫损伤进入植物中时，伤口诱导型启动子可以用于本发明的构建中。这种伤口诱导型启动子包括马铃薯蛋白酶抑制剂(pin II)基因(Ryan(1990)Ann.Rev.Phytopath.28:425-449；Duan et al.(1996)Nature Biotechnology 14:494-498)；wun1和wun2，美国专利号5,428,148；win1和win2(Stanford et al.(1989)Mol.Gen.Genet.215:200-208)；系统素(McGurl et al.(1992)Science 225:1570-1573)；WIP1(Rohmeier et al.(1993)Plant Mol.Biol.22:783-792；Eckelkamp et al.(1993)FEBS Letters 323:73-76)；MPI基因(Corderok et al.(1994)Plant J.6(2):141-150)等，这些文献以引用方式并入本文。
化学诱导型启动子可以通过应用外源化学调控剂用于调制植物中基因的表达。根据目的，启动子可以为化学诱导型启动子，其中化学物质的应用诱导基因表达；或者为化学抑制型启动子，其中化学物质的应用抑制基因表达。化学诱导型启动子是本领域已知的，并且包括但不限于玉米In2-2启动子，其被苯磺酰胺除草剂安全剂活化；玉米GST启动子，其被用作出土前使用的除草剂的疏水性亲电子化合物活化；以及烟草PR-1a启动子，其被水杨酸活化。其他的目的化学调节型启动子包括固醇类反应性启动子(如参见Schena et al.(1991)Proc.Natl.Acad.Sci.USA 88:10421-10425和McNellis et al.(1998)Plant J.14(2):247-257中的糖皮质激素诱导型启动子)，以及四环素诱导型和四环素抑制型启动子(例如参见Gatz et al.(1991)Mol.Gen.Genet.227:229-237,和美国专利号5,814,618和5,789,156)，这些文献以引用方式并入本文。
所述表达盒还包含用于选择转化的细胞的选择性标志物基因。选择性标志物基因用于选择转化的细胞或组织。标志物基因包括编码抗生素抗性的基因，例如编码新霉素磷酸转移酶II(NEO)和潮霉素磷酸转移酶(HPT)的那些；以及赋予抵抗除草化合物的抗性的基因，所述除草化合物例如草胺磷、溴苯腈、咪唑啉酮和2,4-二氯苯氧乙酸酯(2,4-D)。其他的选择性标志物包括表型标志物，例如β-半乳糖苷酶；以及荧光蛋白，例如绿色荧光蛋白(GFP)(Su et al.(2004)Biotechnol Bioeng 85:610-9and Fetter et al.(2004)PlantCell 16:215-28)，青色荧光蛋白(CYP)(Bolte et al.(2004)J.Cell Science 117:943-54and Kato et al.(2002)Plant Physiol 129:913-42)，和黄色荧光蛋白(来自的EvrogenPhiYFPTM，参见Bolte et al.(2004)J.Cell Science 117:943-54)。对于其他的选择性标志物，通常参见Yarranton(1992)Curr.Opin.Biotech.3:506-511；Christopherson et al.(1992)Proc.Natl.Acad.Sci.USA 89:6314-6318；Yao et al.(1992)Cell 71:63-72；Reznikoff(1992)Mol.Microbiol.6:2419-2422；Barkley et al.(1980)in The Operon,pp.177-220；Hu et al.(1987)Cell 48:555-566；Brown et al.(1987)Cell 49:603-612；Figge et al.(1988)Cell 52:713-722；Deuschle et al.(1989)Proc.Natl.Acad.Aci.USA86:5400-5404；Fuerst et al.(1989)Proc.Natl.Acad.Sci.USA 86:2549-2553；Deuschleet al.(1990)Science 248:480-483；Gossen(1993)Ph.D.Thesis,University ofHeidelberg；Reines et al.(1993)Proc.Natl.Acad.Sci.USA 90:1917-1921；Labow etal.(1990)Mol.Cell.Biol.10:3343-3356；Zambretti et al.(1992)Proc.Natl.Acad.Sci.USA 89:3952-3956；Baim et al.(1991)Proc.Natl.Acad.Sci.USA88:5072-5076；Wyborski et al.(1991)Nucleic Acids Res.19:4647-4653；Hillenand-Wissman(1989)Topics Mol.Struc.Biol.10:143-162；Degenkolb et al.(1991)Antimicrob.Agents Chemother.35:1591-1595；Kleinschnidt et al.(1988)Biochemistry 27:1094-1104；Bonin(1993)Ph.D.Thesis,University of Heidelberg；Gossen et al.(1992)Proc.Natl.Acad.Sci.USA 89:5547-5551；Oliva et al.(1992)Antimicrob.Agents Chemother.36:913-919；Hlavka et al.(1985)Handbook ofExperimental Pharmacology,Vol.78(Springer-Verlag,Berlin)；Gill et al.(1988)Nature 334:721-724。这些公开文献以引用方式并入本文。
上述选择性标志物基因的列举无意于进行限定。任何选择性标志物基因都可以用于本发明。
用于转化植物的多种植物转化载体和方法是可利用的。例如参见An,G.et al.(1986)Plant Pysiol.,81:301-305；Fry,J.,et al.(1987)Plant Cell Rep.6:321-325；Block,M.(1988)Theor.Appl Genet.76:767-774；Hinchee,et al.(1990)Stadler.Genet.Symp.203212.203-212；Cousins,et al.(1991)Aust.J.PlantPhysiol.18:481-494；Chee,P.P.and Slightom,J.L.(1992)Gene.118:255-260；Christou,et al.(1992)Trends.Biotechnol.10:239-246；D’Halluin,et al.(1992)Bio/Technol.10:309-314；Dhir,et al.(1992)Plant Physiol.99:81-88；Casas et al.(1993)Proc.Nat.Acad Sci.USA 90:11212-11216；Christou,P.(1993)In VitroCell.Dev.Biol.-Plant；29P:119-124；Davies,et al.(1993)Plant Cell Rep.12:180-183；Dong,J.A.and Mchughen,A.(1993)Plant Sci.91:139-148；Franklin,C.I.andTrieu,T.N.(1993)Plant.Physiol.102:167；Golovkin,et al.(1993)Plant Sci.90:41-52；Guo Chin Sci.Bull.38:2072-2078；Asano,et al.(1994)Plant Cell Rep.13；AyeresN.M.and Park,W.D.(1994)Crit.Rev.Plant.Sci.13:219-239；Barcelo,et al.(1994)Plant.J.5:583-592；Becker,et al.(1994)Plant.J.5:299-307；Borkowska et al.(1994)Acta.Physiol Plant.16:225-230；Christou,P.(1994)Agro.Food.Ind.Hi Tech.5:17-27；Eapen et al.(1994)Plant Cell Rep.13:582-586；Hartman,et al.(1994)Bio-Technology 12:919923；Ritala,et al.(1994)Plant.Mol.Biol.24:317-325；和Wan,Y.C.and Lemaux,P.G.(1994)Plant Physiol.104:3748。
本发明的方法涉及将多核苷酸构建体引入植物中。“引入”是指将多核苷酸构建体以使得该构建体接近植物细胞内部的方式呈递给植物。本发明的方法不依赖于用于将多核苷酸构建体引入植物中的特定的方法，仅依赖于多核苷酸构建体接近植物至少一个细胞的内部。用于将多核苷酸构建体引入植物中的方法是本领域已知的，包括但不限于稳定的转化方法、瞬时转化方法和病毒介导的方法。
“稳定的转化”是指引入植物中的多核苷酸构建体整合至植物的基因组中，并且能够被其后代继承。“瞬时转化”是指引入植物中的多核苷酸构建体不整合至植物的基因组中。
就植物和植物细胞的转化而言，使用标准的技术将本发明的核苷酸序列插入本领域已知的适用于在植物或植物细胞中表达核苷酸序列的任何载体中。载体的选择取决于优选的转化技术，以及待转化的靶植物物种。
用于构建植物表达盒并且将外来核酸引入植物中的方法是本领域公知的，并且之前已经描述。例如可以使用肿瘤诱导型(Ti)质粒载体将外来DNA引入植物中。用于外来DNA传递的其他方法涉及PEG介导的原生质体转化、电穿孔、微注射须(microinjectionwhiskers)、以及用于直接DNA吸收的基因枪或微粒轰击的使用。这种方法是本领域已知的。(Vasil等人的美国专利号5,405,765；Bilang et al.(1991)Gene 100:247-250；Scheid etal.,(1991)Mol.Gen.Genet.,228:104-112；Guerche et al.,(1987)Plant Science 52:111-116；Neuhause et al.,(1987)Theor.Appl Genet.75:30-36；Klein et al.,(1987)Nature 327:70-73；Howell et al.,(1980)Science 208:1265；Horsch et al.,(1985)Science 227:1229-1231；DeBlock et al.,(1989)Plant Physiology 91:694-701；Methods for Plant Molecular Biology(Weissbach and Weissbach,eds.)AcademicPress,Inc.(1988)和Methods in Plant Molecular Biology(Schuler and Zielinski,eds.)Academic Press,Inc.(1989))。转化的方法取决于待转化的植物细胞、所用的载体的稳定性、基因产物的表达水平以及其他参数。
将核苷酸序列引入植物细胞并随后插入植物基因组中的其他合适的方法包括Crossway et al.(1986)Biotechniques 4:320-334所述显微注射；Riggs et al.(1986)Proc.Natl.Acad.Sci.USA 83:5602-5606所述电穿孔；Townsend等人的美国专利号5,563,055,Zhao等人的美国专利号5,981,840所述土壤杆菌介导的转化；Paszkowski et al.(1984)EMBO J.3:2717-2722所述直接基因转移；以及在例如Sanford等人的美国专利号4,945,050；Tomes等人的美国专利号5,879,918；Tomes等人的美国专利号5,886,244；Bidney等人的美国专利号5,932,782；Tomes et al.(1995)“Direct DNATransfer into IntactPlant Cells via Microprojectile Bombardment,”in Plant Cell,Tissue,and OrganCulture:Fundamental Methods,ed.Gamborg and Phillips(Springer-Verlag,Berlin)；McCabe et al.(1988)Biotechnology 6:923-926)；和Lec1transformation(WO 00/28058)的基因枪颗粒加速。还参见Weissinger et al.(1988)Ann.Rev.Genet.22:421-477；Sanford et al.(1987)Particulate Science and Technology 5:27-37(洋葱)；Christouet al.(1988)Plant Physiol.87:671-674(大豆)；McCabe et al.(1988)Bio/Technology6:923-926(大豆)；Finer and McMullen(1991)In Vitro Cell Dev.Biol.27P:175-182(大豆)；Singh et al.(1998)Theor.Appl.Genet.96:319-324(大豆)；Datta et al.(1990)Biotechnology 8:736-740(水稻)；Klein et al.(1988)Proc.Natl.Acad.Sci.USA 85:4305-4309(玉米)；Klein et al.(1988)Biotechnology6:559-563(玉米)；Tomes的美国专利号5,240,855；Buising等人的美国专利号5,322,783和5,324,646；Tomes et al.(1995)“Direct DNATransfer into Intact Plant Cells via Microprojectile Bombardment,”in Plant Cell,Tissue,and Organ Culture:Fundamental Methods,ed.Gamborg(Springer-Verlag,Berlin)(玉米)；Klein et al.(1988)Plant Physiol.91:440-444(玉米)；Fromm et al.(1990)Biotechnology 8:833-839(玉米)；Hooykaas-Van Slogteren etal.(1984)Nature(London)311:763-764；Bowen等人的美国专利号5,736,369(谷物)；Bytebier et al.(1987)Proc.Natl.Acad.Sci.USA 84:5345-5349(百合科)；De Wet etal.(1985)in The Experimental Manipulation of Ovule Tissues,ed.Chapman et al.(Longman,New York),pp.197-209(花粉)；Kaeppler et al.(1990)Plant Cell Reports9:415-418and Kaeppler et al.(1992)Theor.Appl.Genet.84:560-566(须介导的转化)；D’Halluin et al.(1992)Plant Cell 4:1495-1505(电穿孔)；Li et al.(1993)Plant CellReports 12:250-255以及Christou and Ford(1995)Annals of Botany 75:407-413(水稻)；Osjoda et al.(1996)Nature Biotechnology 14:745-750(玉米，通过根癌土壤杆菌)，所有这些文献均以引用方式并入本文。
本发明的多核苷酸可以通过使植物与病毒或病毒核酸接触而被引入至植物中。通常，这种方法涉及将本发明的多核苷酸构建体引入至病毒DNA或RNA分子中。此外，应该认识到本发明的启动子还涵盖用于通过病毒RNA聚合酶转录的启动子。将多核苷酸构建体引入植物中并表达其所编码的蛋白质的方法(涉及病毒DNA或RNA分子)是本领域已知的。例如参见美国专利号5,889,191、5,889,190、5,866,785、5,589,367和5,316,931；这些文献以引用方式并入本文。
如果需要，可以在配制物中制备修饰的病毒或修饰的病毒核酸。这种配制物以已知的方式制备(例如参见US 3,060,084,EP-A707 445(用于液体浓缩物),Browning,“Agglomeration”,Chemical Engineering,Dec.4,1967,147-48,Perry’s ChemicalEngineer’s Handbook,4th Ed.,McGraw-Hill,New York,1963,pages 8-57以及下列等等：WO 91/13546,US 4,172,714,US 4,144,050,US 3,920,442,US 5,180,587,US 5,232,701,US 5,208,030,GB 2,095,558,US 3,299,566,Klingman,Weed Control as a Science,John Wiley and Sons,Inc.,New York,1961,Hance et al.Weed Control Handbook,8thEd.,Blackwell Scientific Publications,Oxford,1989and Mollet,H.,Grubemann,A.,Formulation technology,Wiley VCH Verlag GmbH,Weinheim(Germany),2001,2.D.A.Knowles,Chemistry and Technology of Agrochemical Formulations,KluwerAcademic Publishers,Dordrecht,1998(ISBN 0-7514-0443-8)，例如通过使用适用于农用化学品配制物的辅助物质延伸活性化合物，例如溶剂和/或载体，如果需要可以使用乳化剂、表面活性剂、分散剂、防腐剂、消泡剂、防冻剂，对于种子处理配制物而言，还任选地使用着色剂和/或粘结剂和/或胶凝剂。
在特定的实施方案中，可以使用本领域已知的多种瞬时转化方法将本发明的多核苷酸构建体和表达盒提供给植物。这种方法包括例如显微注射或颗粒轰击。例如参见Crossway et al.(1986)Mol Gen.Genet.202:179-185；Nomura et al.(1986)PlantSci.44:53-58；Hepler et al.(1994)PNAS Sci.91:2176-2180和Hush et al.(1994)J.Cell Science 107:775-784，所有这些文献以引用方式并入本文。备选地，可以使用本领域已知的技术，将多核苷酸瞬时转化至植物中。这种技术包括病毒载体系统和根癌土壤杆菌介导的瞬时表达，如本发明其他处所述。
已经转化的细胞可以根据传统的方式生长成植物。例如参见McCormick et al.(1986)Plant Cell Reports 5:81-84。然后，这些植物可以生长，并且使用相同转化的菌株或不同的菌株授粉，并且所得到的杂交物具有经鉴定的所需表型特征的构成型表达。可以生长2代或更多代，以确保所需的表型特征的表达被稳定地保持，遗传，然后得到收获后确保所需的表型特征被表达的种子。按照这种方式，本发明提供了转化的种子(还称为“转基因种子”)，其具有稳定地引入至基因组中的本发明的多核苷酸构建体，例如本发明的表达盒。
用于修饰植物基因组中的DNA的本领域已知的任何方法都可以用于原位改变植物中的R基因的编码序列，例如将同源的易感等位基因的核苷酸序列改变为提供抵抗秆锈病的至少一个物种的抗性的等位基因的核苷酸序列。这种方法包括基因组编辑技术，例如涉及靶向诱变、同源重组和突变育种的方法。靶向诱变或相似的技术在美国专利号5,565,350；5,731,181；5,756,325；5,760,012；5,795,972,5,871,984和8,106,259中公开，所有这些文献的全文以引用方式并入本文。用于基因修饰或基团替代(包括同源重组)的方法可以涉及诱导DNA中的双键断裂，使用锌指核酸酶(ZFN)，TAL(转录激活子样)效应物核酸酶(TALEN)，规律成簇的间隔短回文重复序列(Clustered Regularly Interspaced ShortPalindromic Repeats)/CRISPR相关的核酸酶(CRISPR/Cas核酸酶)，或者归位内切酶(经改造的核酸内切酶，使得植物、其他有机体或宿主细胞的基因组中的特异性识别序列的双链断裂)。例如参见Durai et al.,(2005)Nucleic Acids Res 33:5978-90；Mani et al.(2005)Biochem Biophys Res Comm 335:447-57；美国专利号7,163,824,7,001,768和6,453,242；Arnould et al.(2006)J Mol Biol 355:443-58；Ashworth et al.,(2006)Nature 441:656-9；Doyon et al.(2006)J Am Chem Soc 128:2477-84；Rosen et al.,(2006)Nucleic Acids Res 34:4791-800；和Smith et al.,(2006)Nucleic Acids Res34:e149；美国专利申请公开号2009/0133152；和美国专利申请公开号2007/0117128，所有这些文献的全文以引用方式并入本文。
TAL效应物核酸酶(TALEN)可以用于使植物基因组中的特异性识别序列处的双链断裂，从而通过同源重组用于基因修饰或基因替代。TAL效应物核酸酶为可以用于使植物或其他有机体基因组中特异性靶序列处的双链断裂的一类序列特异性核酸酶。TAL效应物核酸酶是通过使天然的或改造的转录激活子样(TAL)效应物或其功能部分与核酸内切酶(例如FokI)的催化结构域融合而创建的。独特的模块化TAL效应物DNA结合结构域使得可以设计具有潜在的任何给定DNA识别特异性的蛋白质。因此，TAL效应物核酸酶的DNA结合结构域可以改造成识别特异性DNA靶位点，并由此用于在所需的靶序列处使双链断裂。参见WO2010/079430；Morbitzer et al.(2010)PNAS 10.1073/pnas.1013133107；Scholze andBoch(2010)Virulence 1:428-432；Christian et al.Genetics(2010)186:757-761；Li etal.(2010)Nuc.Acids Res.(2010)doi:10.1093/nar/gkq704；和Miller et al.(2011)Nature Biotechnology 29:143–148，所有这些文献均以引用方式并入本文。
CRISPR/Cas核酸酶系统还可以用于产生植物基因组中特异性识别序列处的双链断裂，从而通过同源重组用于基因修饰或基因替代。CRISPR/Cas核酸酶为RNA向导的(简单向导RNA，简称为sgRNA)DNA核酸内切酶系统，其在与所设计的RNA同源的DNA链段中使得序列特异性双链断裂。还可以设计序列的特异性(Cho S.W.et al.,Nat.Biotechnol.31:230-232,2013；Cong L.et al.,Science 339:819-823,2013；Mali P.et al.,Science 339:823-826,2013；Feng Z.et al.,Cell Research:1-4,2013)。
此外，ZFN可以用于产生植物基因组中特异性识别序列处的双链断裂，从而通过同源重组用于基因修饰或基因替代。锌指核酸酶(ZFN)为融合蛋白质，其包含用于DNA切割的FokI限制性核酸内切酶蛋白质的部分以及锌指蛋白质，所述锌指蛋白质识别特异性的设计的基因组序列，并且切割在这些序列中的双链DNA，由此产生游离的DNA末端(Urnov F.D.etal.,Nat Rev Genet.11:636-46,2010；Carroll D.,Genetics.188:773-82,2011)。
使用位点特异性核酸酶(例如本发明上文所述那些)断裂DNA可以增加断裂区中同源重组的速率。因此，将上文所述这种效应物与核酸酶偶联能够在包含加入、删除和其他修饰的基因组中生成靶向的变化。
本发明的核酸分子、表达盒、载体和多核苷酸构建体可以用于任何植物物种的转化，包括但不限于单子叶植物和双子叶植物。本发明的优选的植物为小麦植物。其他目的植物物种的实例包括但不限于辣椒(辣椒属物种；例如甜辣椒(Capsicum annuum)、灯笼辣椒(C.baccatum)、中华辣椒(C.chinense)、灌木状辣椒(C.frutescens)、绒毛辣椒(C.pubescens)等)、番茄(Lycopersicon esculentum)、烟草(Nicotiana tabacum)、茄子(Solanum melongena)、矮牵牛(矮牵牛属物种；例如Petunia x hybrida或Petuniahybrida)、豌豆(Pisum sativum)、豆(菜豆(Phaseolus vulgaris))、苞米或玉米(Zeamays)、芸苔属物种(例如(B.napus)、芜青(B.rapa)、芥菜(B.juncea))、特别是用作种籽油来源的那些芸苔属物种、苜蓿(Medicago sativa)、水稻(Oryza sativa)、黑麦(Secalecereale)、高粱(Sorghum bicolor、Sorghum vulgare)、稷(例如珍珠稷(Pennisetumglaucum)、黍稷(Panicum miliaceum)、粟(Setaria italica)、龙爪稷(穇子(Eleusinecoracana))、向日葵(Helianthus annuus)、红花(Carthamus tinctorius)、大豆(Glycinemax)、烟草(Nicotiana tabacum)、马铃薯(Solanum tuberosum)、花生(Arachishypogaea)、棉花(海岛棉(Gossypium barbadense、Gossypium hirsutum))、甘薯(Ipomoeabatatus)、木薯(Manihot esculenta)、咖啡(咖啡属物种)、椰子(Cocos nucifera)、菠萝(Ananas comosus)、广柑树(柑橘属物种)、可可(Theobroma cacao)、茶(Camelliasinensis)、香蕉(香蕉属物种)、鳄梨(Persea americana)、无花果(Ficus casica)、番石榴(Psidium guajava)、芒果(Mangifera indica)、橄榄(Olea europaea)、番木瓜(Caricapapaya)、腰果(Anacardium occidentale)、澳洲坚果(Macadamia integrifolia)、扁桃(Prunus amygdalus)、甜菜(Beta vulgaris)、甘蔗(甘蔗属物种)、棕榈、燕麦、大麦、蔬菜、观赏植物和针叶树。
如本文所用，术语“植物”包括植物细胞、植物原生质体、可以再生成植物的植物细胞组织培养物、植物愈伤组织、植物丛以及在植物或植物的部分中是完好的植物细胞(例如胚芽、花粉、胚珠、种子、块茎、繁殖体、叶、花、枝、果实、根、根尖、花药等)。再生的植物的后代、变体和突变体也包含在本发明的范围内，只要这些植物包含引入的多核苷酸即可。如本文所用，“后代”和“后代植物”包括植物的任何后代，而不论其是由有性生殖和/或无性生殖得到的，除非内容中另外明确地陈述，或者由使用内容显而易见。
在本发明的一些实施方案中，使用编码本发明的R蛋白质的多核苷酸构建体转化植物细胞。如本文所用，术语“表达”是指基因产物的生物合成，包括所述基因产物的转录和/或翻译。由DNA分子“表达”或“生产”蛋白质或多肽是指编码序列的转录和翻译，从而产生蛋白质或多肽，而由RNA分子“表达”或“生产”蛋白质或多肽是指RNA编码序列的翻译，从而产生蛋白质或多肽。编码R蛋白质的多核苷酸构建体和核酸分子的实例在本发明的其他处描述。
术语“DNA”或“RNA”在本发明中的使用无意于将本发明限制为包括DNA或RNA的多核苷酸分子。本领域的那些普通技术人员将认识到本发明的方法和组合物涵盖这样的多核苷酸分子，其包含脱氧核糖核苷酸(即，DNA)、核糖核苷酸(即，RNA)或者核糖核苷酸和脱氧核糖核苷酸的组合。这种脱氧核糖核苷酸和核糖核苷酸包括天然形成的分子和合成的类似物，包括但不限于核苷酸类似物或修饰的主链残基或键，其是合成的、天然形成的和非天然形成，其具有与参照核酸相似的结合性质，并且以与参照核苷酸相似的方式代谢。这种类似物的实例包括但不限于磷硫酰酯、氨基磷酸酯、甲基膦酸酯、手性-甲基膦酸酯、2-O-甲基核糖核苷酸、肽-核酸(PNA)。本发明的多核苷酸分子还涵盖多核苷酸分子的所有形式，包括但不限于单链形式、双链形式、发夹、茎环结构等。此外，本领域那些普通技术人员理解的是本发明公开的核苷酸序列还涵盖示例性核苷酸序列的成分。
本发明描绘了用于增强植物抵抗植物疾病的抗性的组合物和方法，特别是用于增强小麦植物抵抗小麦秆锈病的抗性的组合物和方法。“疾病抗性”是指植物避免由于植物-病原体相互作用的结果而导致的疾病症状。换言之，防止病原体导致植物疾病和相关的疾病症状，或者备选地，由病原体导致的疾病症状减少或减轻。
本发明涵盖了本发明公开的多核苷酸构建体，或者随附的序列表和/或附图，包括但不限于SEQ ID NO:138、139、140、141、142、143、144、145、146、147、148、149、150、151、152、153、154、155、156、157、158、159、160、161和162。本发明进一步涵盖了包含至少一种这种多核苷酸构建体的植物、植物细胞、宿主细胞和载体，以及由这种植物产生的食物产品。此外，本发明涵盖了包含至少一种这种多核苷酸构建体的植物在本发明其他处公开的方法中的用途，例如用于增强小麦植物抵抗小麦秆锈病的抗性的方法，以及在农业农作物生产中限制小麦秆锈病的方法。
以下实施例通过示意性的方式提供，但不是限定。
实施例
实施例1：Sr22和Sr45的基因图位
Sr22被导入至得自二倍体A-基因组相关的野生一粒小麦的小麦染色体7A中(Gerechter-Amitai et al.(1971)Euphytica 20:281-285；The(1973)Nature-NewBiol.241:256)。在培育品种Schomburgk中，Sr22在全世界赋予抵抗市售可得的秆锈病重要物种的抗性，包括Ug99物种组，并且是有效抵抗Yemeni和Ethiopian分离物的少数抗性基因之一(图1；表1)。但是，Sr22的部署受到限定，这是由于关联基因的等位基因所赋予的差的农业性能(The et al.(1988)Proceedings of 7th International Wheat GeneticsSymposium,Miller and Koebner eds.,Bath Press,Bath,UK,pp.901-909)，并且我们试图使用标准的基于图谱的方法在小麦中克隆Sr22由于受抑制的重组而受挫(图3)。
我们通过将Schomburgk(一种六倍体小麦品系，其携带得自野生一粒小麦编号G-21的Sr22(Paull et al.(1994)Theor.Appl.Genet.89:1039-1045))与秆锈病易感栽培品种Westonia杂交而形成制图(mapping)群体。根据Kota等人的方法(Kota et al.(2006)Theor.Appl.Genet.112:492-499)，我们筛选1150个F2种子，其具有密切关联的侧翼SSR标志物cfa2123和cfa2019(Khan et al.(2005)Theor.Appl.Genet.111:846-850)，并鉴定180个重组子。使所选的重组子的种子发展至F3代，并使用Periyannan等人所述的方法和分离物针对锈病应答进行测试(Periyannan et al.(2011)Theor.Appl.Genet.122:1-7)。随后，使用cssu22(Periyannan et al.(2011)Theor.Appl.Genet.122:1-7)，染色体7A特异性小麦表达的序列标签(wESTs)BE443521、BG274853、BE498985、BG262287、BF201318、BG604641(Akhunov et al.(2010)BMC Genomics 11:702))以及衍生自同源染色体7D(Luo et al.(2013)PNAS 110:7940-7945)的标志物AT7D7092和AT7D7094筛选180个重组子品系的DNA。发现标志物BG274853和BE498985处于远端，并且分别与Sr22分隔3个和1个重组事件，而发现标志物BE443521和AT7D7094处于近端，并且分别与Sr22分隔8个和2个重组事件。发现剩余的标志物cssu22、BG262287、BF201318和BG604641与Sr22共分隔(图3)。
除了六倍体群体以外，使用1150个F2植物补充Sr22基因座作图，其中所述1150个F2植物是由栽培一粒小麦编号PI190945(携带Sr22)(Rouse and Jin(2011)Phytopathology101:1418-1423)和秆锈病易感编号PI272557杂交衍生得到的。我们使用标志物BE498985和AT7D7094，并回收重组子(图3)。通过使用小麦秆锈病菌分离物06ND76C(物种QFCSC)筛选，验证重组子中Sr22的存在或缺乏，其中所述分离物06ND76C的特征在于对具有Sr22的品系是无毒力的(Rouse and Jin(2011)Phytopathology 101:1418-1423)。发现，由Schomburgkx Westonia群体得到的共分隔标志物在PI190945和PI272557之间均不是多态性的。
表1.Sr22TB(Schomburgk)和栽培一粒小麦(PI190945)(携带针对全世界范围的秆锈病分离物的集合的Sr22)的秆锈病感染类型(IT)
*物种细节和感染类型得自Rouse and Jin 2011。具有感染得分的品系；并且0至2+(分别为高到低水平的抗性)分类为抗性，而高于3分分为易感品系(Phytopathology 101:1418-1423；Plant Dis.95:941-944)和Olson et al.2010(Crop Science 50:1823-1830)。
#Sr22TB和Schomburgk携带得自相同来源(野生一粒小麦编号G-21)的Sr22(Paullet al.(1994)Theor.Appl.Genet.89:1039-1045；Olson et al.(2010)Crop Science 50:1823-1830)。
^类似于抵抗物种98-1,2,3,5和6的抗性应答，当与感染类型3+的易感品系Morocco相比时，六倍体品系Schomburgk也显示抵抗其他澳大利亚秆锈病物种(即，34-1,2,3,4,5,6,7；34-1,2,3,6,7,8,9；34-1,2,3,5,7,8,9；34-1,2,7+Sr38；40-1,2,3,4,5,6,7；98-1,2,3,5,6,7和17-1,2,3,7)的相似的感染类型。
为了对Sr45作图，使用在Periyannan et al.(2014)Theor.Appl.Genet.127:947-955中所述高分辨率作图群体(CS1D5406x Chinese Spring)。亲代小麦品系CS1D5406(携带Sr45的Chinese Spring 1D替换；Jones et al.(1991)Genome 34:505-508)与ChineseSpring(CS-易感亲代)杂交，从而生成1150个F2种子的高分辨率作图家族。由CS1D5406/CS高分辨率作图家族得到的421个F2种子是简单重复序列标志物gwm106和gwm337的重组子，并且用于构建Sr45的连锁图。421个种子的胚芽部分发育成F3，并且表型为幼苗抗性。使用其他的基于小麦EST的标志物(BE444266和BE446624)，Sr45区间(interval)进一步缩减至23个重组子(图4)。在缺乏密切连锁的EST的情况下，使用选择性扩增引物对(PstI+ATT和MseI+GAA)的AFLP分析扩增与抗性品系有关的片段，并作图为以0.39cM的距离接近Sr45(AFLP标志物AF45)。密切连锁的(AF45)片段用于鉴定BAC克隆HB079K08，其形成重叠群ctg2981的部分(得自University of California,Davis的小麦D基因组物理作图项目)，其中用于诊断Sr45的序列加标签标志物cssu45由所述重叠群ctg2981的部分发展而来(Periyannan et al.,2014Theor Appl Genet 127:947-955)。
实施例2：Ren-Seq+EMS由大基因组克隆抗性基因的用途
基于以下方面，使用3步法快速克隆R基因：(i)化学诱变和针对易感突变体的筛选；(ii)外显子捕获和下一代测序；然后(iii)野生型与突变体的序列比较。如下文所述，这种3步法用于由17Gb六倍体面包小麦基因组克隆广谱秆锈病抗性基因Sr22和Sr45。
大部分的植物R基因属于结构种类的基因，其编码核苷酸结合和富含亮氨酸重复(NB-LRR)结构域(Dangl et al.(2013)Science 341:746-751)。典型的植物基因组包含几百个NB-LRR同源物(“NB-LRRome”)，大部分的同源物存在于复合簇中(Hulbert et al.(2011)Annu.Rev.Phytopathol.39:285-312)。对植物基因组的这种较小的特定区域的富含R基因的测序(RenSeq)可以通过使用生物素化的RNA寡核苷酸由Illumina文库捕获片段，其中所述RNA寡核苷酸设计成与参照基因组的NB-LRR基因库互补(Jupe et al.(2013)PlantJ.76,530-544)。重要的是，对马铃薯群体(分割用于疾病抗性)所采用的RenSeq可以鉴定NB-LRR中特征连锁的单一核苷酸多态性(SNP)(Jupe et al.(2013)Plant J.76,530-544)。最近以来，RenSeq型方法(称为“RenSeq+EMS”)通过使用近-等基因变异(通过针对易感突变体筛选甲磺酸乙酯(EMS)-诱变的群体而获得，所述群体衍生自单一的抗性亲代)，而不使用NB-LRRome比较中2个不同的栽培品种，用于由小麦鉴定R基因(2015年2月20日提交的美国专利申请No.14/667,116和2015年2月20日提交的PCT/US2015/016792，这两份文献的全文均以引用方式并入本文)。通过使用RenSeq+EMS，相对直接地获得所述这种突变体，这是因为R基因抑制剂筛选通常在R基因的突变中产生大约90％突变体，而非第二位点抑制剂基因(B.Wulff,未公开的分析)。
实施例3：EMS突变体群体的产生
我们对Schomburgk(携带Sr22)和CS1D5406(携带Sr45)的种子进行Periyannan等人所述EMS诱变(Periyannan et al.(2013)Science 341:786-788)。在杀菌曲线分析中，0.3％EMS被鉴定为导致50％死亡率和生长减少的最佳剂量。然后，我们使用这种浓度分别处理CS1D5406和Schomburgk的2000和3000个种子，并使植物发展至M2代。我们收获680和1300个单头(由EMS处理的CS1D5406和Schomburgk得到的M2家族)，并针对对澳大利亚秆锈病物种40-1,2,3,4,5,6,7(PBI培养编号383)的应答筛选这些种子。由不同的M2家族的秆锈病筛选，我们鉴定6种易感突变体(1S、2S、3S1、5S1、6S和7S1)(对于Sr22)(图1)和6种突变体(49S1、52S1、55S1、57S1、59S1和61S1)(对于Sr45)，它们在M3代中再次证实(图2)。
实施例4：富集文库的设计
我们使用得自MYcroarray(参见互联网：mycroarray.com/)的专用版本设计120个核苷酸的60000个诱饵(bait)。源序列衍生自公共可得的基因注释(International WheatGenome Sequencing Consortium(2014)Science 345:1251788；Mayer et al.(2012)Nature 491:711-716)和Ae.sharonensis(EBI|PRJEB5333)的其他的基因组原始数据。在Ae.sharonensis的情况下，我们使用RNASeq数据(EBI|PRJEB5340)，从而根据Mayer等人(Mayer et al.(2012)Nature 491:711-716)重新预测基因模型。我们包含了所有含有NB-ARC(Pfam|PF00931)结构域的基因，意识到这可能包含了其他的基因家族。使用pfam_scan，针对NB-ARC结构域筛选翻译的蛋白质序列(Finn et al.(2014)Nuc.Acids Res.42:D222-230)。将所有预测的属于所选基因的外显子加入至源序列组中。短于120bp的外显子向上游和下游延长60bp。所得的序列通过以下过程进一步精制：(i)使用RepeatMasker(可得自互联网：repeatmasker.org/)和Triticeae Repeat library TREP(可得自互联网：wheat.pw.usda.gov/ITMI/Repeats/)针对重复元件进行筛选；(ii)使用CD-HIT(Fu et al.(2012)Bioinformatics 28:3150-3152)聚类(一致性阈值为95％)，从而除去冗余的序列；以及(iii)确保序列不具有反向互补基序。最后一步是通过使用BLASTn(Zhang et al.(2000)Comput.Biol.7:203-214)将所有的序列针对所有的序列进行比对，并重新定向冲突的序列而取得的。
实施例5：DNA提取和文库制备
使用Lagudah等人所述方法(Lagudah et al.(1991)Genome 34:375-386)，我们由EMS突变体的叶组织提取总基因组DNA。使用NanoDrop分光光度计(ThermoFisherScientific)和Quant-iT PicoGreen dsDNA测定法(Life Technologies)进行DNA定量。将DNA样品归一化至3μg，并在Covaris S2 Focused-超声发生器中剪切成平均长度为500bp。通过凝胶电泳测定小的等分物，并使用Agencourt AMPure XP珠(Beckman CoulterGenomics)进行其他尺寸的选择。将样品进行末端修复，然后使用NEBNext Ultra DNALibrary Prep Kit for Illumina(New England Biolabs)进行3'dA添加。将Illumina测序接头连接至末端，并使用AMPure XP珠进行随后的纯化，再使用索引的PCR引物(NEBNextMultiplex Oligos for Illumina,New England Biolabs)和Illumina PE1.0PCR引物对DNA进行PCR扩增(6轮)。在使用AMPure XP珠进行纯化后，使用Bioanalyzer DNA 1000芯片(Agilent)和PicoGreen测定法进行定量测定法，从而确定平均片段尺寸和浓度。
实施例6：Illumina文库的靶物富集和测序
根据MYbaits(MYcroarray)方案并使用MYbaits试剂富集DNA文库。简言之，在65℃将500ng制备的文库在杂交缓冲剂(10x SSPE,10x Denhardt溶液,10mM EDTA,0.2％SDS)中与生物素化的RNA诱饵杂交40h。杂交后，使用链霉亲和素涂敷的磁珠按照如下方式回收结合的DNA：将杂交混合物加入洗涤3次并再次悬浮于结合缓冲剂(1M NaCl；10mM Tris-HCl,pH 7.5；1mM EDTA)中的Dynabeads MyOne Streptavidin C1(Invitrogen,LifeTechnologies)中。在RT 30min后，使磁珠沉淀，并在RT使用1x SSC/0.1％SDS洗涤1次15min，然后在65℃使用0.1x SSC/0.1％SDS洗涤3次，每次10分钟。使用0.1M NaOH洗脱捕获的DNA，并使用1M Tris-HCl,pH 7中和。使用AMPure XP珠纯化文库，并使用Q5HighFidelity DNA Polymerase(New England Biolabs)以及Illumina P5(5’AATGATACGGCGACCACCGA 3；SEQ ID NO:58)和P7引物(5’CAAGCAGAAGACGGCATACGA 3’；SEQID NO:59)进行PCR扩增(14-16个循环)。将富集的文库在Illumina MiSeq或HiSeq平台上，以TGAC进行末端配对测序(表2)。
表2.针对Sr22和Sr45测序的文库
1基于Bioanalyzer。
2与NB-LRR相关。
3重叠群上的TopHat作图。仅包含源BLAST命中≤1E-50的读值。
实施例7：NB-LRR读值组装及比较
使用CLC assembly cell(www.clcbio.com/products/clc-assembly-cell/)和标准的参数重头组装得自野生型的原始数据。使用BWA(Li and Durbin(2009)Bioinformatics 25:1754-1760)将各突变体和野生型的原始数据与野生型组装进行比对。使用SAMtools(Li et al.(2009)Bioinformatics 25:2078-2079)和参数-f 2，将所得的SAM文件过滤用于读值作图作为合适的对。使用SAMtools Mpileup将结果转化成累积格式。使用MegaBLAST(Zhang et al.(2000)Comput.Biol.7:203-214)将重头组装的野生型重叠群与诱饵文库的源序列进行比对。仅考虑与源序列具有局部比对的重叠群的子序列用于进一步的分析。使用累积格式，鉴定潜在的突变位置。如果由野生型原始数据针对野生型组装的作图衍生得到的局部覆盖率为至少50倍，并且突变体的备选等位基因的频率为至少10％，则考虑所述位置用于进一步的分析。后一步被设想为在极端敏感的，并且一定能够鉴定大量的假阳性位置，这些位置在随后的步骤中被滤出。因为2个独立突变的植物在相同的位置处具有突变是非常不可能的，则在超过1个突变体中发现的每个位置都被滤出。平均覆盖率低于中值总体覆盖率10％的区域被认为是该品系的删除突变。所得的候选重叠群通过子序列与诱饵源序列进行局部比对中SNP或删除突变的数量而进行评级。
对于Sr22，我们鉴定27个在2种突变体中突变的重叠群，以及单一的3,408 bp重叠群，其在5种突变体中包含独立的突变(contig_10555_1)(图5)。对于Sr45，鉴定在2种或更多种突变体中包含独立的突变的28个重叠群，但是仅一个5,266bp重叠群在6种突变体中具有突变(contig_1795_1)(图6)。
实施例8：通过BLAST检索、全转录组测序和长距离PCR连接Sr22 5'和3'重叠群
BLAST检索(Zhang et al.(2000)Comput.Biol.7:203-214)：使用BLASTx将Sr225'候选重叠群(contig_10555_1)与NCBI非冗余蛋白质比对。最佳命中为得自节节麦(gi|475585845|gb|EMT20152.1)的疾病抗性基因类似物(RGA)的3'末端。然后，我们取得没有被contig_10555_1覆盖的RGA的5'末端，并使用tBLASTn检索我们的Sr22野生型组装。最佳命中contig_3841_1覆盖节节麦RGA的剩余5'部分，并且该重叠群包含Sr22突变体2S中的非同义突变。通过对各突变体的PCR和Sanger测序，我们还证明突变的存在。所有6种突变均为GC至AT过渡，其导致无义(2个)或错义(4个)突变。
RNASeq：使用Sickle(可得自互联网：github.com/najoshi/sickle)在一定品质下剪裁得自Sr22野生型(Schomburgk)的全转录组测序的末端配对读值。使用BLASTn(Zhanget al.(2000)Comput.Biol.7:203-214)，将剪裁的读值与Sr22(contig_10555_1)的3'重叠群比对。然后，将具有局部比对(≥99％一致性)和它们的配对物的所有读值与组装的其他重叠群比对。接着，通过读值最佳比对的次数对所有重叠群进行评分。
为了确认Sr22的完整的编码序列，使用Tophat2(Kim et al.(2013)GenomeBiol.14:R36)，将转录组读值针对最终的基因座进行作图。使用Tablet(Milne et al.(2013)Brief.Bioinform.14:193-202)手工检查比对。
长距离PCR：使用Phusion High-Fidelity DNA Polymerase(New EnglandBiolabs Inc.)以及引物对S22F14(5’GCGAACTTTACTGTCAACGG 3’；SEQ ID NO:91)和S22R4(5’CAAAGATCTCATCGCGACG 3’；SEQ ID NO:92)，通过PCR扩增进一步证明Sr22开放阅读框的全长序列。将PCR产物克隆至pCR-XL-TOPO载体中(Life Technologies)，随后测序。
实施例9：局部靶向富集和基因组步移
为了获得Sr22的启动子和终止子区域，我们使用修改的RenSeq方法，将其称为局部RenSeq。这种方法使用长的生物素化的RNA探针(>500nt)，其靶向组装的重叠群的已知5’和3’末端，从而由基因组DNA文库富集长片段(>2kb)。使用这种方法，我们克隆2.5kb启动子(图5)。基本上如上文中标准的RenSeq所述(具有最少的修改)，构建用于局部RenSeq的基因组DNA文库。使用gTUBE遵循用于5kb的制造商的设定，剪切4μg基因组DNA，并且使用NEBNextUltra DNA Library Prep Kit for Illumina(P5-P7接头)以延长的扩增PCR时间(5分钟)从而可以扩增较长的DNA片段，由2μg构建文库。如上文中标准的RenSeq文库所述，实施所有的QC步骤。
为了生成对于Sr22的5'具有特异性的生物素化的RNA探针，我们由Sr22基因组DNA进行PCR扩增对应于5'候选重叠群(contig_10555_1)的第一600nt，并将其克隆至处于T7启动子之下的pGEM-T Easy载体(Promega)中。为了生成run-off转录物，使用PstI(NEB)将具有克隆的片段的质粒线性化，并使用the MAXIscript T7Transcription Kit(LifeTechnologies)以60％UTP和40％Biotin-16-UTP(Cambio)体外合成生物素化的RNA。纯化生物素化的RNA，并使用RNA Clean and Concentrator-5kit(Zymo Research)进行浓缩，再使用Nanodrop定量。
将合成的生物素化的5'探针与长插入物的文库以1:1比例混合，并且如上文中标准的RenSeq所述进行杂交。为了进一步富集启动子序列，以用于文库构建的相同的设置使用Illumina P5正向引物(SEQ ID NO:58)和反向引物(5’TGCGCTCACCAGAACTTCCGCCATTGTTGC 3’；SEQ ID NO:136)实施富集后PCR，其中所述引物对5'候选物重叠群(在contig 3841_1中的位置163-192)具有特异性。在1％琼脂糖凝胶上运行PCR产物，并切除2至4kb的片段，使用Nucleospin Extract Kit(Marchery-Nagel)进行纯化，再将其克隆至Zero Blunt PCR Cloning载体(Life Technologies)中。在转化后，我们使用P5(SEQ ID NO:58)和Sr22特异性引物筛选30个细菌集落，并得到6个阳性集落。克隆的片段的长度为1至2.5kb。将6种插入物进行Sanger测序，发现所有的插入物都具有一致的重叠序列，并且最长的克隆片段为2,545bp。为了证明克隆的片段属于Sr22启动子，我们PCR扩增2.5kb片段，其包含1.6kb新克隆的启动子和ATG的800bp下游，即，涵盖2S突变[使用引物KW_sr22_p_seq_F1(5’CAAGGCTGCTATTGCTGTGTTTGGCCCTGA 3’；SEQ ID NO:62)和KW_sr22_p_seq_R1(5’GAGCGTGGTCTTGCCCAACCCTCCATAT 3’；SEQ ID NO:63)]。在得自野生型和2S突变体的基因组DNA上实施PCR，得到单链，将其进行直接的Sanger测序。野生型PCR产物的测序证明假定的启动子的序列，同时得自S2的PCR产物的序列包含突变。
随后，还通过基因组步移技术鉴定Sr22和Sr45的启动子(在Sr22情况下，证明启动子序列)和终止子序列。就基因组步移技术而言，使用APAgeneTMGOLD Genome Walking Kit(Bio SandT Inc.,Canada)根据制造商的方案扩增在cDNA起始位点以上的上游区域。在Sr22基因序列的5’区域设计的引物S22R13(5’GTTCTTGAGCAGCTTGTATTCATCCG 3’；SEQ IDNO:64)、S22R12(5’CAACATAGCTCCCAACTTCCG 3’；SEQ ID NO:65)和S22R11(5’ACTTCCGCCATTGTTGCTCTC 3’；SEQ ID NO:66)用于扩增ATG上游的区域，而得自3’区域的S22F35(5’CTCCTTCTAATTAGATGCATACATTAGAGG 3’；SEQ ID NO:67)、S22F36(5’GTAGCTCAAACCGTCGGTCC 3’；SEQ ID NO:68)和S22F37(5’GATTCGTCGCGATGAGATC 3’；SEQ IDNO:69)引物用于鉴定下游区域。类似地，使用S45F11(5’GGCCTAAACTATGGGAACCAAAATCC 3’；SEQ ID NO:70)、S45F12(5’GGAAATATATGGTTCCTTCACTGCG 3’；SEQ ID NO:71)和S45F13(5’GCAAGCGTGAGTCTGAATAGAGG 3’；SEQ ID NO:72)引物鉴定Sr45基因序列的3’区域。
实施例10：Sr22和Sr45的5'和3'RACE
通过5’和3’RACE提供转录物UTR区域的证明。根据Krattinger等人(Krattingeret al.(2009)Science 323:1360-1363)中所述方法，进行RNA提取、cDNA合成、5'和3'RACE(cDNA末端的快速扩增)。使用在5’区域设计的引物S22R13(5’GTTCTTGAGCAGCTTGTATTCATCCG 3’；SEQ ID NO:64)[用于Sr22]和S45G1(5’CAGTGGCCTGACTACAAACTCC 3’；SEQ ID NO:73)[用于Sr45]进行5’RACE反应，而使用得自3’区域的S22F13(5’CCTGCTTTGGGTTGTATCC 3’；SEQ ID NO:74)[用于Sr22]和S45G3(5’GCTGGATTGCAATAGTATTGAGTCC 3’；SEQ ID NO:75)[用于Sr45]进行3’RACE反应。为了证明剪接结构，使用S22F24(5’GAGAGAGAGAGAGCAACAATGG 3’；SEQ ID NO:76)和S22R39(5’ATAGAATATAGATGCACGCTAGATCTTT 3’；SEQ ID NO:77)[用于Sr22，得自野生一粒小麦]，S22F24(5’GAGAGAGAGAGAGCAACAATGG 3’；SEQ ID NO:76)和S22R37(5’CTGAATTCGTGATACTTGTCTTACTTAT 3’；SEQ ID NO:78)[用于Sr22，得自栽培一粒小麦]，以及S45F7(5’GCCTAACTCCAGACCATTTC 3’；SEQ ID NO:79)和S45R1(5’AACTTGACTCCCTTGAAGAGG3’；SEQ ID NO:80)[用于Sr45]引物对来扩增cDNA。
实施例11：Sr22和Sr45基因与它们各自的遗传基因座的共分隔
为了进一步验证我们克隆了Sr22和Sr45，我们使用所述序列以生成PCR分子标志物，并在高分辨率作图群体中显示与基因基因座的完全连锁(对于Sr22，Schomburgk xWestonia和PI190945x PI272557群体；对于Sr45，CS1D5406x Chinese Spring)(图3-4)。使用序列特异性引物对所鉴定的Sr22基因序列进行作图，[对Schomburgk中Sr22具有特异性的S22F2(5’CGCAGATGGATTTCTGAAGGC 3’；SEQ ID NO:81)/S22R3(5’ATGTGCCAAATATCATAGTCCG 3’；SEQ ID NO:82),对PI190945中Sr22具有特异性的S22F2(SEQID NO:82)/S22R10(5’CAAATAACGGCTTCTGGG3’；SEQ ID NO:83)，对Westonia中Sr22(Schomburgk)的抗性基因类似物具有特异性的S22F2(SEQ ID NO:81)/S22R51(5’ATGGACTCGGCAATACTCC 3’；SEQ ID NO:84)，以及对PI272557中Sr22(Schomburgk)的抗性基因类似物具有特异性的S22F2(SEQ ID NO:81)/S22R22(5’AAGCTCCGTGATTCCTGTAAG 3’；SEQID NO:85)]。为了对Sr45作图，使用对Sr45基因具有特异性的引物对S45F1(5’AGTACTGTAATAATTGATTCCGTCG 3’；SEQ ID NO:86)/S45R1(5’AACTTGACTCCCTTGAAGAGG 3’；SEQ ID NO:80)。
实施例12：Sr22单体型分析
除了Schomburgk中存在的野生一粒小麦的Sr22来源以外，将得自栽培一粒小麦的秆锈病抗性的第二来源导入至其他六倍体小麦背景的染色体7A中，并且基于对秆锈病物种的一致特异性，也称为Sr22。六倍体小麦W3534(Paull et al.(1994)Theor.Appl.Genet.89:1039-1045)是野生一粒小麦衍生的Sr22的原型，并且在栽培一粒小麦集合的随后广泛分析中，多个编号(包括PI190945)被推测为Sr22的载体。已经证明PI190945具有单显性基因，所述单显性基因独立于除了Sr22以外的栽培一粒小麦秆锈病抗性基因而被分隔(Rouse and Jin(2011)Phytopathology 101:1418-1423)。除了用于构建作图群体的4个亲代品系以外，使用表3所示的32个栽培一粒小麦编号、16个野生一粒小麦编号(得自ICARDA)、DV92和W3534(Paull et al.(1994)Theor.Appl.Genet.89:1039-1045)鉴定Sr22基因序列的变体。使用2个重叠引物对来扩增Sr22相关的序列：S22F25(5’AACAATGGCGGAAGTTCTG 3；SEQ ID NO:87)和S22R16(5’ATGTTCTCTTCAGAGGATTTGTCA 3’；SEQID NO:88)，以及S22F2(SEQ ID NO:81)和S22R36(5’CGTGATACTTGTCTTACTTATTCCTAGTC 3’；SEQ ID NO:89)。就Westonia而言，使用引物对S22F29(5’CCGTGCTGTATACCATGTGA 3’；SEQID NO:90)和S22R51(SEQ ID NO:84)代替S22F25和S22R16。我们获得得自PI190945的对应Sr22序列，并且其与在二倍体作图群体(PI190945x PI272557)中位于与在Schomburgk中的直系同源位点所发现的相同位置处的抗性表型共分隔(图3)。此外，显示W3534和PI190945具有一致的Sr22样基因序列。然而，它们与Schomburgk中的参照Sr22基因序列具有33个氨基酸改变(图10A-10P)，表明对于这些基因型在Sr22基因座处的等位基因变异。得自栽培一粒小麦编号PI289605和PI330550的其他序列(其显示与PI190945相似的针对小麦秆锈病菌物种的应答)(表3)是彼此一致的，但是在7个氨基酸位置处与PI190945和W3534不同。在抗性野生一粒小麦编号(IG44857和IG44921)和Schomburgk的序列之间，也发现相似的氨基酸改变，而得自第三野生一粒小麦编号IG44855的序列与Schomburgk是几乎一致的，除了在于N末端区域的2个氨基酸删除(图10A-10P)。意外的是，具有低秆锈病感染的栽培一粒小麦编号PI573523的序列与易感野生一粒小麦编号IG44878的序列密切相关，表明抗性得自除了Sr22以外的基因座的可能性。总之，发现大量氨基酸可以提供二倍体A基因组类群(taxa)之间的差异，但是得自栽培一粒小麦的一种秆锈病易感编号的序列与野生一粒小麦抗性基因型归为一组，表明中间类群或错误分类的可能性(图7)。
表3.用于鉴定Sr22变体的二倍体编号(A基因组)和六倍体小麦
实施例13：Sr22和Sr45构建体的创建
由小麦(普通小麦)cv Schomburgk分离基因组片段7.815kb，其携带由野生一粒小麦渗入的Sr22秆锈病抗性基因。使用引物S22F14(5'GCGAACTTTACTGTCAACGG 3'；SEQ IDNO:91)+S22R4(5'CAAAGATCTCATCGCGACG 3'；SEQ ID NO:92)，以及Phusion High-FidelityDNA Polymerase(New England Biolabs Inc.)在制造商建议的条件下扩增所述片段。类似地，还使用S22F43(5’CAATTTCATCAAGACATGCG3’；SEQ ID NO:93)和S22R24(5’GATGTTATTTAATTATGGTTTGGCC 3’；SEQ ID NO:94)引物，由栽培一粒小麦编号PI190945扩增相应的Sr22序列。就Sr45而言，使用S45F1(5’AGTACTGTAATAATTGATTCCGTCG3’；SEQ ID NO:86)和S45R5(5’GAAATTCCTGCTGCATTGC3’；SEQ ID NO:137)引物，由节节麦编号AUS18911扩增包含Sr45的6.8kb基因组片段。将Sr22基因组片段克隆至二元载体pVecBARII(SEQ IDNO:163)的NotI位点中，所述二元载体是pWBvec8(Wang et al.(1998)Acta Hortic.461:401-405)的衍生物，其中，35S潮霉素基因已经被衍生自pCMneoSTL2的35S NPTII选择性标志物基因替代(Maas et al.(1997)Mol.Breed.3:15-28)。
通过GoldenGate克隆生成Sr22和Sr45二元构建体
1.Sr22和Sr45成分的设计
就Sr22而言，3种启动子(包括玉米泛素、Sr33启动子区和Sr22-PI190945启动子区)用于所述设计。得自3种不同来源(包括Sr22-Schomburgk、PI190945和PI573523)的Sr22编码区(由起始密码子至终止密码子的序列，包括外显子和内含子)用于所述设计。为了使构建体的合成简便，将编码区分成2个部分，即，编码区1和2。3个终止子区域(包括Sr22-Schomburgk、Sr22-PI190945和Sr33终止子)用于所述设计。
就Sr45而言，Sr45编码区用于所述设计。
就GoldenGate克隆而言，选择BsaI限制性核酸内切酶。因此，驯化(domesticate)所有的BsaI位点(即，在DNA合成之前，通过编辑除去)，而不改变编码区的外显子中预测的氨基酸编码序列并且不改变内含子剪接供体/受体位点。在Sr45的情况下，我们也除去单一的BpiI位点，而不改变预测的氨基酸编码序列。使用程序Geneious 7.1.7进行BsaI位点(在Sr45的情况下为BpiI)的驯化。加入序列AATG以连接启动子和编码区，并且加入GCTT以连接编码区和终止子。加入序列ACGT以连接编码区1和2。加入GGAG和CGCT以连接载体的左边界和右边界。
总共设计11个构建体，9个用于Sr22，而1个3个用于Sr45(表4)。
此外，设计并合成一种完整的合成基因，其BsaI位点未驯化，并且由与Sr22编码区融合的Sr33启动子构成，其中所述Sr22编码区与Sr33终止子融合。
为了有利于将合成基因转移至二元载体vecBAR II中，在启动子的起始处和终止子的终止处引入NotI位点。
2.构建体的合成
由市售可得的DNA合成提供者合成所有这些工具包构建体。载体PUC57中接受Sr22工具包序列，而载体pMS中接受Sr45构建体。
3.工具包构建体的质粒拯救
将包含工具包序列的质粒稀释100倍，并根据制造商的方案转化至得自LifeTechnology Ltd的SUBCLONING EFF DH5COMPETENT CELLS中。将50μl的10倍稀释的转化的DH5铺展在具有抗生素选择的平板上。将单一的集落挑选至50ml圆锥管中的10mL BL培养基中并过夜培养。使用NucleoSpin Plasmid(Macherery-Nagel)，根据制造商的方案提取质粒。
4.GoldenGate克隆法
通过GoldenGate克隆，根据以下方案生成总共用于Sr22-Schomburgk的3个合成基因(启动子、编码区和终止子的组合)、用于Sr22-PI190945的2个合成基因、用于Sr22-PI573523的2个合成基因以及用于Sr45的3个合成基因(表4)：
a.100ng受体质粒-pICH47732用于各个反应。
b.针对浓度测试包含待插入的各部分的质粒。摩尔比例为2:1的插入物:受体质粒用于所有的启动子-编码区-终止子的组合。
c.2.0μl T4连接酶缓冲剂(10x,NEB)，2μl牛血清白蛋白(10x)，0.5μl 400U/μlT4DNA连接酶(200units,NEB)和0.5μl 10U/μl BsaI(5个单位,NEB)用于各反应。反应时间表为：
37℃，20秒
然后进行如下的26个循环：
37℃，3min
16℃，4min
接着，
50℃，5min
80℃，5min
10℃，储存。
根据制造商的方案，将5μl反应物用于转化至Life Technology Ltd的DH5COMPETENT CELLS中。将50-100μl转化的DH5铺展至包含羧苄青霉素(50mg/ml)、IPTG(0.1mM)、X-Gal(20ug/ml)的平板上。在包含羧苄青霉素(50mg/ml)的LB培养基中培养白色集落。将5ml培养物沉淀下来，并使用NucleoSpin Plasmid(Macherey-Nagel)根据所述公司的方案提取质粒。
表4.用于8个Sr22和3个Sr45合成基因的启动子-编码区-终止子的组合
1制备分离的Sr22合成基因用于表4中列出的各编码区。例如使用SEQ ID NO:141中列出的Sr22 Schomburgk编码区构建第一Sr22-1合成基因，并使用SEQ ID NO:142中列出的Sr22Schomburgk编码区构建第二Sr22-1合成基因。
使用程序Primers 3设计总共41种引物，用于检查由GoldenGate克隆得到的合成的Sr22和Sr45基因的序列(表5)。
表5.41种引物的序列
5.将合成的基因转移至二元载体vecBAR II中
通过上文所用的相同的方案回收载体vecBAR II(第3点)。使用NotI消化vecBARII质粒，然后使用Antarctic Phosphatase(NEB)根据所述公司的方案将所述质粒脱磷酸化。
使用NotI，根据所述公司的方案消化得自GoldenGate克隆的包含合成的Sr22和Sr45基因的质粒。在琼脂糖凝胶上运行所消化的质粒，并且切除插入物条带，再使用QIAquick Gel purification Kit根据所述公司的方案进行纯化。接着，使用DNA T4连接酶，根据所述公司的方案连接Sr22和Sr45合成的基因(插入物)和脱磷酸化的vecBAR II。取得5μl连接反应物，转化至Life Technology Ltd的DH5COMPETENT CELLS中。将50-100μl转化的DH5铺展在包含壮观霉素(50mg/ml)的平板上。在包含壮观霉素(50mg/ml)的LB培养基中培养单一的白色集落。使5ml培养物沉淀下来，并使用NucleoSpin Plasmid(Machery-Nagel)根据所述公司的方案提取质粒。将包含所需的插入物的二元质粒转化至用于小麦转化的根癌土壤杆菌中。
实施例14：使用Sr22和Sr45转化小麦
如Ishida et al.(2014)“Wheat(Triticum aestivum L.)Transformation UsingImmature Embryos,”in“Agrobacterium Protocols:Volume 1,”Methods in MolecularBiology 1223:199-209所述进行小麦的土壤杆菌转化。使用24℃，16h光/18℃，8h暗生长策略，在温室生长条件下使小麦栽培品种Fielder植物繁殖，并使用水溶胶(Aquasol)每2周一次对植物进行施肥。在开花期标记小麦头，并如Ishida等人(2014)所述并在CSIRO,Canberra,Australia(Richardson et al.(2014)Plant Cell Tiss.Organ Cult.119:647-659)中采用的那样，在开花期后12-14天收获小麦头用于转化试验。简言之，将种子在0.8％次氯酸钠溶液中进行表面消毒10min。在无菌条件下除去种子的胚芽，并与包含Sr22和Sr45基因组片段二元构建体的土壤杆菌菌种在WLS-AS培养基上在黑暗中共同培养2天(Ishidaet al.(2014)“Wheat(Triticum aestivum L.)Transformation Using ImmatureEmbryos,”in“Agrobacterium Protocols:Volume 1,”Methods in Molecular Biology1223:199-209)。在使用手术刀切除共同培养的胚轴后，将外植体随后转移至WLS-Res培养基中，并在24℃放置于黑暗中。在5天后，将外植体转移至包含5mg/ml草丁膦(PPT)的WLS-P5愈伤组织诱导培养基中用于愈伤组织形成。
2周后，将所得的愈伤组织一分为二，并在黑暗中在WLS-P10(10mg/l PPT)上放置3周。然后，在24℃在200μmols m-2s-1光照下，愈伤组织在LSZ-P5(5mg/l PPT)培养基上再生。将幼苗转移至LSF-P5(5mg/l PPT)培养基中，使根形成，并且一旦健壮的根系统发育形成，则将植物转移至温室中。
实施例15：Sr22和Sr45转化子对于抵抗秆锈病的评分
为了对Sr22和Sr45介导的抗性的存在情况进行评分，使用秆锈病抗性物种98-1,2,3,5和6感染得自T0和T1代的转基因品系。接种后2周，评估植物的抗性。具有抗性至中等抗性感染类型的植物的特征在于小至中等尺寸的夏孢子堆具有由萎黄病包围的绿色小岛。预计抗性和中等抗性感染类型与转基因植物中的转基因共分隔。相反，高感染类型的植物的特征为大的夏孢子堆，并且无萎黄病。预计高感染类型不与转基因植物中的Sr22或Sr45转基因共分隔。
实施例16：对转基因小麦中Sr22和Sr45介导的抗性的验证
将分离的构建体克隆至pVecBARII二元载体的NotI位点中，其中所述分离的构建体包含得自Schomburgk的Sr22、得自PI190945的Sr22、得自PI573523的Sr22或Sr45，并且具有天然的或非天然的调控元件(表6)，然后将所述构建体转移至土壤杆菌中，用于转化小麦栽培品种Fielder，基本如实施例14所述。针对抵抗澳大利亚秆锈病物种98-1,2,3,5和6的抗性，筛选转基因小麦品系，基本如实施例15所述，并且结果示于图11A-11E中。
表6.用于产生转化的植物的构建体和转化子表型的描述
1列出通过驯化由启动子区(P)、编码区(C)和终止子区(T)除去的限制性核酸内切酶位点。天然的表示没有位点被驯化。
2R＝抗性，S＝易感
图11A示出使用构建体PC103转化的易感栽培品种Fielder的结果，其中所述构建体PC103包含得自Schomburgk(野生一粒小麦)的Sr22，并具有天然的Sr22 5’和3’调控元件。该图示出6种转基因植物(编号PC103-1、-2、-3、-4、-5、-6)。发现所有的植物都对澳大利亚秆锈病物种98-1,2,3,5和6具有抗性，类似于栽培品种Schomburgk中的Sr22应答(感染型2-)。
图11B示出使用构建体PC129转化的易感栽培品种Fielder的结果，其中所述构建体PC129包含得自PI190945(栽培一粒小麦)的Sr22，并具有天然的5’和3’调控元件。总共回收13种转基因植物，并针对抵抗澳大利亚秆锈病物种98-1,2,3,5和6的抗性进行评分。植物编号PC129-1、-4、-6、-7、-8、-10、-11、-13具有类似于PI190945中的Sr22应答(感染型2-)的抗性。所有这8种植物中，图像中显示PC129-1、-4、-6、-11和-13。一种植物(PC129-9)具有感染型2(稍微高于PI190945)，而3种植物(PC129-2、-5和-12)具有感染型2+(高于PI190945)。一种植物(PC129-3)具有感染型3+，其类似于Fielder。
图11C示出使用构建体PC110转化的易感栽培品种Fielder的结果，其中所述构建体PC110包含得自节节麦编号AUS18911的Sr45，并具有天然的5’和3’调控元件。总共回收12种转基因植物，并针对抵抗澳大利亚秆锈病物种98-1,2,3,5和6的抗性进行评分。七种植物(PC110-1、-2、-4、-5、-7、-10、-12)具有感染型1，其类似于AUS18911和CS1D5406中的Sr45。4种植物(PC110-3、-6、-9、-11)具有感染型1(稍微高于AUS18911)。一种植物(PC110-8)具有感染型3+，其类似于Fielder。
图11D-11E示出由转基因植物得到的结果，其中所述转基因植物包含得自Schomburgk的Sr22，得自PI190945的Sr22，以及得自PI573523的Sr22，并具有天然的或非天然的调控元件。使用构建体PC126、PC127、PC128、PC130、PC131、PC132、PC146和PC147转化易感栽培品种Fielder。用于各构建体的启动子、编码区和终止子列于表6中。对于各构建体而言，针对抵抗澳大利亚秆锈病物种98-1,2,3,5和6的抗性，对10种转基因植物进行评分。在图11D中，衍生自PC126、PC127、PC128、PC130和PC132的植物均具有感染型1+至2的抗性，而衍生自PC131的植物均是易感的并具有感染型3+。各构建体的代表性植物示于图片中。在图11E中，衍生自PC146的所有植物均是高度易感的，而衍生自PC147的植物(除了品系9)均产生感染型1，其为Sr45的典型的强抗性反应。
冠词“一个”和“一种”在本文中用于指一个或多于一个(即，至少一个)的冠词的语法对象。例如“一个元件”是指一个或多个元件。
在整个说明书中，词语分词形式的“包含”或其变体(例如动词形式的“包含”或分词形式的“包含”)应该理解为意味着涵盖所陈述的元件、整数或步骤，或者元件、整数或步骤的组，但是不包含任何其他的元件、整数或步骤，或者元件、整数或步骤的组。
在本说明书中提及的所有公开和专利申请表明了本发明所属技术领域的那些技术人员的水平。所有公开和专利申请均以引用方式并入本文，如同每一份公开或专利申请特意地且单独地表明以引用方式并入本文。
尽管为了达到清楚理解的目的，通过示意和实例相当详细地描述了上述发明，但是显而易见的是某些变化和修改可以在所附的权利要求书的范围内实施。
序列表
<110> Two Blades Foundation
Commonwealth Scientific and Industrial Research
Organisation
<120> 小麦秆锈病抗性基因及使用方法
<130> IIC180270
<141> 2016-08-03
<150> US 62/200,894
<151> 2015-08-04
<160> 163
<170> SIPOSequenceListing 1.0
<210> 1
<211> 9859
<212> DNA
<213> 普通小麦
<400> 1
ccgcccagca acgccgcccc tgtccccggg cgcccgccgc cgccgggaag caaactagtt 60
cccgccgccg cttcccccac cgcacagccg cccgcgacca agaagacgcc tcgccacccc 120
cgccacatac gaccgacacg ctcgtcggac gccgtcacac tcgtcggacg caggcagggc 180
agctagttgg tctgtgggcg ccgcgtctcc cctcaccggc cgtctccttc ttctacgccc 240
gcaagctgtt cgacagtttg ccaaggtacg aaaatgaact ccgccgatga gtgttttttc 300
cacaatttca tttgcgactc cgacgattcg tcgtccgacg acgaggagga gatattggct 360
gccatgttgg tccatcacca cctcaacagc cagcggccgt tgttccgtgg ctccataccg 420
ggccaccttc ccgacgttga accgcaatcg agagagcggg catttccttc ttcggaagga 480
ctactttgat acaacaaacc cgttgttcaa acatcaaaaa ttccgtcgtc gtttccgtat 540
gagtaggcat cttttcaacc gtattagaga gggggtggtc ggctatgatg actattgcga 600
gtgaaagagg atgccgtcgg caagatcggt ttctcctctt atcagaaatg cactgccgcc 660
atccgaatgc ttgcatacgg agtgcacggt gatctcattg acgagtacgt ccgtatgagt 720
gagtctacat gcctagagtc cctgtataag ttctgcaagg ctgctattgc tgtgtttggc 780
cctgagtact tgagaaagcc gacaactaaa gatacatctt gtttgttggc gacgaatgcc 840
agaaggggct ttccagggat gcttggcagc atagactgca tgcactggga gtggaagaac 900
tgtccttctg cttggcaagg gcaatataag ggacatgtca gggcttgcgc tgtcatacta 960
aggccgtgac atctcaagat ctctggatct ggcactcttt ctttggcatg gctggatcac 1020
acaatgatat caacgtgctt cagcgctcgc gggtgtttgc taggcttgcc gaaggcaaca 1080
gcccaccggc gaactttact gtcaacggac acaactacga caaacgatac tacctgggtg 1140
atagtatcta tcctagtgga ccactattgt caagataata cccaaccctg tcggagagaa 1200
gaggaaaaga tttgcccatg agcaagagag tgctaggaag gatgtcgagc gtgcctttgg 1260
tgttttgcaa tctcgatggg gcatcgttcg gtatgctgct aatatctgga gcacgcagaa 1320
actgtgggag gtgatgactg cttgtgtgat catgcataat atgatcgtag aagacgagcg 1380
accggaacgt ctgtacgatc aagggtttca gtttcagagt gagaatgttg tgcctgagca 1440
tggagtagca gcaacgtttg aacagttcat ccaatttcat caagacatgc gtgattggga 1500
aactcacgtg cagccgcaaa atgatttggt tgagtatatg tggggtcatg ttggcaacca 1560
atagatatat cttcttttat tcgtttgcaa aactatgaaa catttatttt tatttttatt 1620
tgtatccggc ttgtaaaact atactatttt atttggtaaa ctatgttatt tgacaatatg 1680
tttgaatgca aatcaatata atattgggcg gccagccagc cacgccgaca cgtataggtc 1740
gacgcgttgg gcgcgctgcc gactcaaatc taaaaaaggg cggacgccgg gcgggcgggc 1800
gacccaaacg aacaaaaagc gaatgaaatc gccgtccatt tggatcgctc catcggagtt 1860
gctctgtctg gccgccaaat ggggacgtga cggacaccga gtgaactcgt gcggtgtgtc 1920
cgcttcacat ctaaatttag gtaaaaaaat gaatccgtat ggacatgttt tgagaatggg 1980
tcgttacgtt gcgacaatat ttttatccac aacgacctaa acaaatgcgg gcagatgaaa 2040
tgtatggctg cgttggagat gctgttaacg gagcaaagtc gttggtagtc ggaccgagca 2100
aagtaattgc tgaggcaggt agtggacgag actgtagaaa ggttccacgc ttaatatctt 2160
gagcagtact tgtagcacat gtgtccctgc aaagctgtct ccgtgctgta taccatgtga 2220
attgcttttc tcaaaagtag tggagtaccc taccgtgttt cattagtctt gccggcttca 2280
tagacaaagg attctttttt cccgtgccat cgccatctgc aagcccaaat caacacacac 2340
cagagagaga gagagagaga gagagagaga gagagcaaca atggcggaag ttctggtgag 2400
cgcatccaca ggggcaatgg gttccctcct gcggaagttg ggagctatgt tgacggatga 2460
atacaagctg ctcaagaacg tccgtgggga catcaagttc ctcaaggacg agctggaggt 2520
catgtgcgcg ttcctcctca agatgtcaga tgtggaggag cctgacgaac caacaaagct 2580
gcgtgtgacg gcggtgcggg agatgtccta taagatagag gacaacatcg acaagttcat 2640
ggtcctcgtg gaacaggaac acgggtcctc ctgttccgag gcggctcatg gcgtcgccaa 2700
gctcatggac aagtgcaaga acttgctgcc ggacatcaag gcccgccgca ggatcgccaa 2760
ggaggtcaaa gacatcaaga aagaaattaa ggatgtcagc gacagatttt caaggtacca 2820
gatcttgttc tccttacagt actattagta cctaccatca gatttgtgac cgatttgttg 2880
ccactcaagg tacaagattg acgactcctc ctcctctatg ccggcaaaag acaaggtcga 2940
ccctcgactt cgtgcagtct acaaagacgc cgcagagctc gtcggaattg atggaccaaa 3000
ggatgaactt gtcaagtggc tgaatgagaa agaaggccaa tcactgaaat ctgtctctat 3060
tgttggatat ggagggttgg gcaagaccac gctcgccaac cagatccggg tcaaccttgg 3120
agcgaccttc gactgcgggg cttttgtctc gatttcacgc aagcctgaca tgaaggcgat 3180
attaagatct atattatcac aaatcaccaa gaaagacgat gcttgctcta gattagatga 3240
tatacaactc atcatcgaca agattcgaga attcctccaa gacacaaggt atgtatgtga 3300
gatctgctac gaagtaacat tgcttaatta gctcatgcat aatcggttgc ctagaatttt 3360
gctaatgaag attgctttgt gtgatttact ttcgtcctca acttgtatga ctgcgcttaa 3420
actttcaagt cattgtttct tgcaacttat gtcgatctgt tcactaatta agatatagct 3480
aaaatgtgtt tgccgatttc tgtcgccaca cattttctaa tttgattgcc acaagtttgt 3540
taaacttgcg gctatgttct gtttattaaa taattgtgac atggcgtcag tcaaaacaat 3600
aaagtacatg caattccctc tcttttgtac aagataaata tccgcacgca ctacatgact 3660
gcaaatatat ctatcttcta aggacccaaa gcacttgcat gtgcagacca ttgttaccgg 3720
atatgcagta cattaggttt gtgattagcc actttaagaa catagggttt ctagaggcta 3780
taaacctacg gttatcagta ttataatctt gaatggatat caaacatccg ttgaagaatc 3840
tatgcccata acacaaaaag agaaaaggga agaattaatc atagtcagat cggaccagcc 3900
tgactagcgt aacataatga caacagtagg ttgttgcgtc tttcctggtt tcgtagcaat 3960
ttgcatgacg aattgaggcg cagagccacc actcaatatg tggtcctacc aatcatgagg 4020
aaataacttg ttaccaaaat gacaaaagaa aataggtagt ccccatcatt gtttcaatat 4080
ctatcctctt gcgaacatgc tattggcagt ggcaaaacat tgtaccttgc cactggcaac 4140
agctgccttt actcggcctg agcattcata tttttttcct cttggttgtt aaatcttgct 4200
atttttcgct tgccttctat ggcaatcttt ttttttctta tattgccacc aaattgtatc 4260
taagagcaac tccaacacgc cgacgcaatt tttgtccatt tagatcggcc aagcggacgg 4320
ggtgtctggt tttgcctttg ggtggtgcgc ctacgcatta gtccgcgtgg gcatttaaaa 4380
aacttaaaaa gcttaaaata attaaatata aataaaaatg catattaaag cccgtcaaag 4440
tccacataat taaacataat acattaatat attaataaaa aacagccgcc ccagcccgca 4500
gccctaggcg tcttcgtcgg aggagtgggt gaggtcgaag aatgccggca gctcagccca 4560
cgggaaggcc tgctggtggg cagccagcac cgcctgctcc gctgtctgcg gcggcggcgg 4620
ccctacctgg caccgcgcac gcctcctcct cgcgctcctg ccgctcctcc tcctcatcgc 4680
acgcccaacg aagttttggc gctcctcccc ctcgggttcc cgctgcatgc ggacctggcc 4740
gtcgtaccgc tcctcctcca gcgtgcggtc cttccacgcc acgaggtagg ccgcctcctc 4800
ctccatcacg cacagccaaa tgggcggctt gctcatccgc ttgcagacga tgccctgcca 4860
ttggtaaacg ccggtgggcg cgggtggtgc tagcgctggt gcaggcggtg acgatgtggc 4920
tggtggtccc ggcaggtaga cactatagct ggcggccgat agctgcagcg tgtcatcgag 4980
gccagaccac taggagtcct cgcactaaga ctcctcggtg acgtgatggg ccccctcctc 5040
caatgccttg aggaacgccg cctcctcctc aacctactat ttgaccaccg gtggcggcgg 5100
tggagggcag gcttcgagct ggacggcgtc gcggaggcgc acatggtgct cgatggtgaa 5160
ccagacttct cagttagggg agtctggcct cgatgggtgg agtcgcgacg ctgctctggc 5220
gtcaggagag cacgccgggc ccgaatctcg ttggcgcgcg cacgctcgga ctgtggcacc 5280
gccggcactg gaatgtggtg ggggtcgagg tgccagccgt gggggaagtt cacgtccggc 5340
cacggcagtg agacgcggta ctcccggtgg tactacgcgt ggtgcaccgg gatgtactgg 5400
tggtcccgtg gcgctgccgc cctcccatcc cgcagcgccg acaccaatgc gaaccccttc 5460
gctgccggcc tcaccggccc ttgcaacgag ccggcctcgt ggtcgtgctt gccttgccct 5520
tacctttgcc aaagaaaccc atgacggcta gggttttctg tcaccggcga gggagcagat 5580
gtggactaga gtgggaggag aagtggatga ggcccgcccc gccgcaccct tgcaattaaa 5640
aagggcgtga tccggacgtt tctagtcact gccatgcggg cccgaggtag gtgggcgcca 5700
ttgactacgt ctgtggtggt agttggctgg cagacacgcc agcccgaggc gcacaccgag 5760
cggacgcgca acacgtccgt ctcgtgttcg tgtgaacgca aatcaggcac aaatttgggc 5820
tggaaatgcg tctacatgga tgccgaacgg acgtgatttg ggtttgtgtc cgcacgttgg 5880
gccggcgttt ttgtgccttt cgaccctaat ggacacacac ggaccaaatc cgttggcgtg 5940
ttagagttgc ttgtacctag tactactatg tgtggagttg ttatctttga gtttctcccc 6000
catctgtagc tgtgtattat tgtgagatgc tctcaggatt tcacttattt tagataatga 6060
caactttctt gatgctagac aattacatca aggtgtatta tatctaggat gcacacagat 6120
gtgagattgt ttcaggattt taggttactt ggtaggtgtt actataaata tctaatgagc 6180
tctaatgtgt atgttcatga aactgtgttg caggtacttt atcataattg atgatatatg 6240
ggagttagga acatgggaaa ctttgaaatg tgcatttgtc aagaatacat tgggcagcag 6300
aataattatc acaacacgta tagttgatgt tgccaaatct tgttctcctt ctagtgaaga 6360
tcttgtctat gagatgaaac cactcagtga ggctgactcg aagaaattgt ttttcaagag 6420
gatatttggc tgtgaagaaa gctgtcctga tagcttgaaa gaagctgcca atgatatttt 6480
gaaaaaatgc cgtggcctac ctctggccat caatgccatt tctagcgtgt tggtcacaac 6540
aagggaaaca aaagaagagt gggatcgggt gcgacattca atacgctctt caaaggtcaa 6600
aagtgatata atagagacca tgaattacat attgtctctt agttattttg atcttcccca 6660
ccacctaaga agctgcttat tgtacctggc tctgtttcct gaagaccaat tgattggaag 6720
gaagcggtta gtacgcagat ggatttctga aggctttatc catggagaaa gtggacaaga 6780
tctcatggaa ttaggagagg aatattttca ccagcttgtg aacagaagtc taatacagcc 6840
cggcaacata gggtatgacg gcaaggcaat gtattgccga gtccatgaca ccatccttga 6900
tttcctaatt gacaaatcct ctgaagagaa catgtgtact gtcctaaaga aacaatgcaa 6960
gcctaatggc atagttcgtc ggctctctct aatggggaat gaggatgaag aaatcgtgga 7020
gcaattggat ctgtcacacg ctcggtcaat cactgctttt ggggatatca agctattgcc 7080
ttcgcttggg aggtcaaaat gcttgcgcgt gctagacttg caagactgcg atcagttgga 7140
aaatcatcat atcaaggata ttgaaagact ctatcagcta agatacttgg atatctcttc 7200
tacaggaatc acggagcttc ccaggcaaat tggggagttg ctgtatctag agacgctagt 7260
tgcctatgga ttacgtgagc ttcctgaaag cacaagtcgg ctacaacgac tggcacgttt 7320
gtttgtttac tctggttgta agcttccagg tgggttggga aacttgataa acttgcaaga 7380
gcttgactgc gttgatgcct tgcatttgaa gcatgtggaa gagctcggca aactaacaaa 7440
tctgagaaag ctgagtatta aattggacac tggtgggatt gaaggcaaca aattagaaga 7500
gtccaaggaa aagctggtgt cctctctctg taaactggac gaatgcggcc tcctttccct 7560
gagtattgat tattatctga gagaaaagga tggagaagag ccgttcctcc ctgctttggg 7620
ttgtatccag gaggtttttg tatatggaca agatatcagc cggattagca gatggctcgc 7680
gtcacttccc aacctacaca ggttgctttt ggacgatccg aagatagagc agcaggatat 7740
tgagatgatt ggattgatac ccaatctgat cgatttgaca ctgccgcctc tatacaaaac 7800
agacgacgct gggcggctca tcatcaggcg tgaaggattt caacagttgc agaagtttga 7860
ggcttacaat actcgtatgg gggttttaat gtttgaaccg ggggctatgc cgaggctcaa 7920
ggagcttaaa ctccataact tcatcgaaaa gcccaaatct gctgcggtcg atttcgactt 7980
tggcatccaa cgcctctcca gccttgctcg cctcactgtc agcttatcct gcggtggctg 8040
gacggtggca gaagtagagg ctgcggagga tgctttcaag agcatggccg aggcgaaccc 8100
caaccgtcca atactggaga tgacaagata taatacgcag catatgttac aagacgagca 8160
aataggtatg acaggctcag gtactattaa ctagtttgta cttttgtctt ggtgagcagt 8220
agatcaaaag atgtgcatat gcttcaatct tttttccggt aatctatttc agctacaacg 8280
ccggccgtca agtcctagat gcctaaaccg agatgcgcct ttggttatgg agcccaagtc 8340
acgtactcgg attggatgct gcagcaaatg atacttctgt acatgtctgc aagtatcacg 8400
aattagtgga atgactagga ataagtaaga caagtatcac gaattcagag tcggatgcaa 8460
tttttttctt ttcttttggg cataagtaga ttgtcatttg agtatttttt tccattaata 8520
tttgtgtatt cttagattga gactttgata tgtaacccag atttctgttg ttaggaaaca 8580
cataccgtgt agcttatcag aagaaaaaga tctagcgtgc atctatattc tatcggacta 8640
tgatatttgg cacatgtgtg ccatataaac aaaactgtca cacctctatt tccttcactc 8700
taaatagatg tatacattag tttgattctt tcttctttca cgtgcatttc tccttctaat 8760
tagatgcata cattagagga caaaaaagac gatgtcattc tagattttct ttattaaaat 8820
atttaaaatg ttttgtagct caaaccgtcg gtccgatgta aaaaccgttt ttacatatac 8880
gattcgtcgc gatgagatct ttgaaactag atcctatgtt gacatgtttc gatgactttt 8940
tttaaccaaa atttaccatg ccttttatat gcaagttacc gtgctatttc cacataagtc 9000
atcggaggca taaaaatggt tcaccgacac tacagttcat atattaccat gatgtttaca 9060
tataagttac cgggaggggg ggggggggtg tctgttttca ataatttccc caccgagtca 9120
aatattacca tagtatatgt acgtaagtta tcaggtatcc gatgggtata ttaccatggc 9180
tatcttttaa aaaaatctct aatttaccgc ggtgtttgta tctaaattat caagtttgtg 9240
gtgcctaatt tactatgcta tttacataca aaagttacca gtgtattttt tgacaatttt 9300
ttactattct tccccaaatc aagactatta ccgtgctgtt tacacacaag ttaccgggta 9360
tattttcaac aacttcatcc acccgaatca aacttatcat gatgtttgtg agtaacttat 9420
catgtctgca atgcgtatat taccattcta tttaaacaaa atttaccaac gatatgcttc 9480
aaacaatttt ttacgtgggt caaagctagc atactgtttt tgcgtaagtt attgtatctg 9540
caataaatat attaccgtgc tatttaccta gaagttattg ggggtaatgt tcttcaacga 9600
aaaaaaattc agggtcaaaa aattattgtg gtgtttgaac gagagttatt aggtctccga 9660
tgggtaaatt atcatgttat ttgcacataa attaccgagg gtatgctttt caacaacttc 9720
ctccatccag gtcaaagtta tcatggtgtt tttgctcaag ttttcaagtt tgtggtgcgt 9780
atattaccac gatgttgact gtttacaagg tttttgagtc gccgactaat cgccgactaa 9840
tcgtcgagtc gtcgcggcg 9859
<210> 2
<211> 941
<212> PRT
<213> 普通小麦
<220>
<221> 结构域
<222> (120)..(147)
<223> 卷曲螺旋结构域
<220>
<221> 结构域
<222> (178)..(465)
<223> 核苷酸结合结构域
<220>
<221> 结构域
<222> (601)..(853)
<223> 富含亮氨酸重复结构域
<400> 2
Met Ala Glu Val Leu Val Ser Ala Ser Thr Gly Ala Met Gly Ser Leu
1 5 10 15
Leu Arg Lys Leu Gly Ala Met Leu Thr Asp Glu Tyr Lys Leu Leu Lys
20 25 30
Asn Val Arg Gly Asp Ile Lys Phe Leu Lys Asp Glu Leu Glu Val Met
35 40 45
Cys Ala Phe Leu Leu Lys Met Ser Asp Val Glu Glu Pro Asp Glu Pro
50 55 60
Thr Lys Leu Arg Val Thr Ala Val Arg Glu Met Ser Tyr Lys Ile Glu
65 70 75 80
Asp Asn Ile Asp Lys Phe Met Val Leu Val Glu Gln Glu His Gly Ser
85 90 95
Ser Cys Ser Glu Ala Ala His Gly Val Ala Lys Leu Met Asp Lys Cys
100 105 110
Lys Asn Leu Leu Pro Asp Ile Lys Ala Arg Arg Arg Ile Ala Lys Glu
115 120 125
Val Lys Asp Ile Lys Lys Glu Ile Lys Asp Val Ser Asp Arg Phe Ser
130 135 140
Arg Tyr Lys Ile Asp Asp Ser Ser Ser Ser Met Pro Ala Lys Asp Lys
145 150 155 160
Val Asp Pro Arg Leu Arg Ala Val Tyr Lys Asp Ala Ala Glu Leu Val
165 170 175
Gly Ile Asp Gly Pro Lys Asp Glu Leu Val Lys Trp Leu Asn Glu Lys
180 185 190
Glu Gly Gln Ser Leu Lys Ser Val Ser Ile Val Gly Tyr Gly Gly Leu
195 200 205
Gly Lys Thr Thr Leu Ala Asn Gln Ile Arg Val Asn Leu Gly Ala Thr
210 215 220
Phe Asp Cys Gly Ala Phe Val Ser Ile Ser Arg Lys Pro Asp Met Lys
225 230 235 240
Ala Ile Leu Arg Ser Ile Leu Ser Gln Ile Thr Lys Lys Asp Asp Ala
245 250 255
Cys Ser Arg Leu Asp Asp Ile Gln Leu Ile Ile Asp Lys Ile Arg Glu
260 265 270
Phe Leu Gln Asp Thr Arg Tyr Phe Ile Ile Ile Asp Asp Ile Trp Glu
275 280 285
Leu Gly Thr Trp Glu Thr Leu Lys Cys Ala Phe Val Lys Asn Thr Leu
290 295 300
Gly Ser Arg Ile Ile Ile Thr Thr Arg Ile Val Asp Val Ala Lys Ser
305 310 315 320
Cys Ser Pro Ser Ser Glu Asp Leu Val Tyr Glu Met Lys Pro Leu Ser
325 330 335
Glu Ala Asp Ser Lys Lys Leu Phe Phe Lys Arg Ile Phe Gly Cys Glu
340 345 350
Glu Ser Cys Pro Asp Ser Leu Lys Glu Ala Ala Asn Asp Ile Leu Lys
355 360 365
Lys Cys Arg Gly Leu Pro Leu Ala Ile Asn Ala Ile Ser Ser Val Leu
370 375 380
Val Thr Thr Arg Glu Thr Lys Glu Glu Trp Asp Arg Val Arg His Ser
385 390 395 400
Ile Arg Ser Ser Lys Val Lys Ser Asp Ile Ile Glu Thr Met Asn Tyr
405 410 415
Ile Leu Ser Leu Ser Tyr Phe Asp Leu Pro His His Leu Arg Ser Cys
420 425 430
Leu Leu Tyr Leu Ala Leu Phe Pro Glu Asp Gln Leu Ile Gly Arg Lys
435 440 445
Arg Leu Val Arg Arg Trp Ile Ser Glu Gly Phe Ile His Gly Glu Ser
450 455 460
Gly Gln Asp Leu Met Glu Leu Gly Glu Glu Tyr Phe His Gln Leu Val
465 470 475 480
Asn Arg Ser Leu Ile Gln Pro Gly Asn Ile Gly Tyr Asp Gly Lys Ala
485 490 495
Met Tyr Cys Arg Val His Asp Thr Ile Leu Asp Phe Leu Ile Asp Lys
500 505 510
Ser Ser Glu Glu Asn Met Cys Thr Val Leu Lys Lys Gln Cys Lys Pro
515 520 525
Asn Gly Ile Val Arg Arg Leu Ser Leu Met Gly Asn Glu Asp Glu Glu
530 535 540
Ile Val Glu Gln Leu Asp Leu Ser His Ala Arg Ser Ile Thr Ala Phe
545 550 555 560
Gly Asp Ile Lys Leu Leu Pro Ser Leu Gly Arg Ser Lys Cys Leu Arg
565 570 575
Val Leu Asp Leu Gln Asp Cys Asp Gln Leu Glu Asn His His Ile Lys
580 585 590
Asp Ile Glu Arg Leu Tyr Gln Leu Arg Tyr Leu Asp Ile Ser Ser Thr
595 600 605
Gly Ile Thr Glu Leu Pro Arg Gln Ile Gly Glu Leu Leu Tyr Leu Glu
610 615 620
Thr Leu Val Ala Tyr Gly Leu Arg Glu Leu Pro Glu Ser Thr Ser Arg
625 630 635 640
Leu Gln Arg Leu Ala Arg Leu Phe Val Tyr Ser Gly Cys Lys Leu Pro
645 650 655
Gly Gly Leu Gly Asn Leu Ile Asn Leu Gln Glu Leu Asp Cys Val Asp
660 665 670
Ala Leu His Leu Lys His Val Glu Glu Leu Gly Lys Leu Thr Asn Leu
675 680 685
Arg Lys Leu Ser Ile Lys Leu Asp Thr Gly Gly Ile Glu Gly Asn Lys
690 695 700
Leu Glu Glu Ser Lys Glu Lys Leu Val Ser Ser Leu Cys Lys Leu Asp
705 710 715 720
Glu Cys Gly Leu Leu Ser Leu Ser Ile Asp Tyr Tyr Leu Arg Glu Lys
725 730 735
Asp Gly Glu Glu Pro Phe Leu Pro Ala Leu Gly Cys Ile Gln Glu Val
740 745 750
Phe Val Tyr Gly Gln Asp Ile Ser Arg Ile Ser Arg Trp Leu Ala Ser
755 760 765
Leu Pro Asn Leu His Arg Leu Leu Leu Asp Asp Pro Lys Ile Glu Gln
770 775 780
Gln Asp Ile Glu Met Ile Gly Leu Ile Pro Asn Leu Ile Asp Leu Thr
785 790 795 800
Leu Pro Pro Leu Tyr Lys Thr Asp Asp Ala Gly Arg Leu Ile Ile Arg
805 810 815
Arg Glu Gly Phe Gln Gln Leu Gln Lys Phe Glu Ala Tyr Asn Thr Arg
820 825 830
Met Gly Val Leu Met Phe Glu Pro Gly Ala Met Pro Arg Leu Lys Glu
835 840 845
Leu Lys Leu His Asn Phe Ile Glu Lys Pro Lys Ser Ala Ala Val Asp
850 855 860
Phe Asp Phe Gly Ile Gln Arg Leu Ser Ser Leu Ala Arg Leu Thr Val
865 870 875 880
Ser Leu Ser Cys Gly Gly Trp Thr Val Ala Glu Val Glu Ala Ala Glu
885 890 895
Asp Ala Phe Lys Ser Met Ala Glu Ala Asn Pro Asn Arg Pro Ile Leu
900 905 910
Glu Met Thr Arg Tyr Asn Thr Gln His Met Leu Gln Asp Glu Gln Ile
915 920 925
Gly Met Thr Gly Ser Ala Thr Thr Pro Ala Val Lys Ser
930 935 940
<210> 3
<211> 2823
<212> DNA
<213> 普通小麦
<400> 3
atggcggaag ttctggtgag cgcatccaca ggggcaatgg gttccctcct gcggaagttg 60
ggagctatgt tgacggatga atacaagctg ctcaagaacg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgcg ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacaggaac acgggtcctc ctgttccgag 300
gcggctcatg gcgtcgccaa gctcatggac aagtgcaaga acttgctgcc ggacatcaag 360
gcccgccgca ggatcgccaa ggaggtcaaa gacatcaaga aagaaattaa ggatgtcagc 420
gacagatttt caaggtacaa gattgacgac tcctcctcct ctatgccggc aaaagacaag 480
gtcgaccctc gacttcgtgc agtctacaaa gacgccgcag agctcgtcgg aattgatgga 540
ccaaaggatg aacttgtcaa gtggctgaat gagaaagaag gccaatcact gaaatctgtc 600
tctattgttg gatatggagg gttgggcaag accacgctcg ccaaccagat ccgggtcaac 660
cttggagcga ccttcgactg cggggctttt gtctcgattt cacgcaagcc tgacatgaag 720
gcgatattaa gatctatatt atcacaaatc accaagaaag acgatgcttg ctctagatta 780
gatgatatac aactcatcat cgacaagatt cgagaattcc tccaagacac aaggtacttt 840
atcataattg atgatatatg ggagttagga acatgggaaa ctttgaaatg tgcatttgtc 900
aagaatacat tgggcagcag aataattatc acaacacgta tagttgatgt tgccaaatct 960
tgttctcctt ctagtgaaga tcttgtctat gagatgaaac cactcagtga ggctgactcg 1020
aagaaattgt ttttcaagag gatatttggc tgtgaagaaa gctgtcctga tagcttgaaa 1080
gaagctgcca atgatatttt gaaaaaatgc cgtggcctac ctctggccat caatgccatt 1140
tctagcgtgt tggtcacaac aagggaaaca aaagaagagt gggatcgggt gcgacattca 1200
atacgctctt caaaggtcaa aagtgatata atagagacca tgaattacat attgtctctt 1260
agttattttg atcttcccca ccacctaaga agctgcttat tgtacctggc tctgtttcct 1320
gaagaccaat tgattggaag gaagcggtta gtacgcagat ggatttctga aggctttatc 1380
catggagaaa gtggacaaga tctcatggaa ttaggagagg aatattttca ccagcttgtg 1440
aacagaagtc taatacagcc cggcaacata gggtatgacg gcaaggcaat gtattgccga 1500
gtccatgaca ccatccttga tttcctaatt gacaaatcct ctgaagagaa catgtgtact 1560
gtcctaaaga aacaatgcaa gcctaatggc atagttcgtc ggctctctct aatggggaat 1620
gaggatgaag aaatcgtgga gcaattggat ctgtcacacg ctcggtcaat cactgctttt 1680
ggggatatca agctattgcc ttcgcttggg aggtcaaaat gcttgcgcgt gctagacttg 1740
caagactgcg atcagttgga aaatcatcat atcaaggata ttgaaagact ctatcagcta 1800
agatacttgg atatctcttc tacaggaatc acggagcttc ccaggcaaat tggggagttg 1860
ctgtatctag agacgctagt tgcctatgga ttacgtgagc ttcctgaaag cacaagtcgg 1920
ctacaacgac tggcacgttt gtttgtttac tctggttgta agcttccagg tgggttggga 1980
aacttgataa acttgcaaga gcttgactgc gttgatgcct tgcatttgaa gcatgtggaa 2040
gagctcggca aactaacaaa tctgagaaag ctgagtatta aattggacac tggtgggatt 2100
gaaggcaaca aattagaaga gtccaaggaa aagctggtgt cctctctctg taaactggac 2160
gaatgcggcc tcctttccct gagtattgat tattatctga gagaaaagga tggagaagag 2220
ccgttcctcc ctgctttggg ttgtatccag gaggtttttg tatatggaca agatatcagc 2280
cggattagca gatggctcgc gtcacttccc aacctacaca ggttgctttt ggacgatccg 2340
aagatagagc agcaggatat tgagatgatt ggattgatac ccaatctgat cgatttgaca 2400
ctgccgcctc tatacaaaac agacgacgct gggcggctca tcatcaggcg tgaaggattt 2460
caacagttgc agaagtttga ggcttacaat actcgtatgg gggttttaat gtttgaaccg 2520
ggggctatgc cgaggctcaa ggagcttaaa ctccataact tcatcgaaaa gcccaaatct 2580
gctgcggtcg atttcgactt tggcatccaa cgcctctcca gccttgctcg cctcactgtc 2640
agcttatcct gcggtggctg gacggtggca gaagtagagg ctgcggagga tgctttcaag 2700
agcatggccg aggcgaaccc caaccgtcca atactggaga tgacaagata taatacgcag 2760
catatgttac aagacgagca aataggtatg acaggctcag ctacaacgcc ggccgtcaag 2820
tcc 2823
<210> 4
<211> 8066
<212> DNA
<213> 栽培一粒小麦
<400> 4
aactacctgg gtgactgtat ttatcctcag tggaccacta ttgtcaagac agtacccaac 60
catgtcggag agaagaggaa aagatttgcc caaaagcaag agagtgctag gaaggatgtc 120
gagcgtgcct ttggtgtttt gcaatctcga tggggcatcg ttcggtatcc tgctaatacc 180
tggagcacgc agaaactgtg ggaggtgatg actgcttgtg tgatcatgca caatatgatc 240
gtagaagaca agcgcccgga acgtctgtac gatcaagggt ttcagtttca ggatgagaat 300
gttgtgcctg agcatggagt agcagcaacg tttgaatagt tcacccaatt tcatcaagac 360
atgcgtgatt gggaaactca cgtgcagctg caaaataatt tgattgagta tatgtgggtc 420
atgttggcaa ccaataaatg tatcttcttt tattcgtttg caaaactatg aaacatttat 480
ttttatttgt atccggtttg taaaactata ctattttatt ctgtaaacta tgttatctga 540
caatatgttt gaatgcaaac taatataata ttgggcggcc aaccggccgc gttggcacgt 600
tgggtgcgct gccgactcaa atctaaaaaa agacggacgc cgggcgggcg gccgacccaa 660
acgaacaaaa agcggacgaa atcgccgtcc atttgggtcg ctccatcgga gttgctctgt 720
ctggccgcca aatggggacg tgacggacac cgagtgaact cgtgcggtgt gtccgcttca 780
catctaaatt taggtaaaaa aatgaatccg tatggacatg ttttgagaat gggtcgttac 840
gttgcgacaa tatttttatc cacaacgacc taaacaaatg cgggcagatg aaatgtatgg 900
ctgcgttgga gatgctgtta acggagcaaa gtcgttggta gtcggaccga gcaaagtaat 960
tgctgaggca ggtagtggac gagactgtag aaaggttcca cgcttaatat cttgagcagt 1020
acttgtagca catgtgtccc tgcaaagctg tctccgtgct gtataccatg tgaattgctt 1080
ttctcaaaag tagtggagta ccctaccgtg tttcattagt cttgccggct tcatagacaa 1140
aggattcttt tttcccgtgc catcgccatc tgcaagccca aatcaacaca caccagagag 1200
agagagagag agagagagag agagagagag agcaacaatg gcggaagttc tggtgagcgc 1260
atccacaggg gcaatgggtt ccctcctgcg gaagttggga gctatgttga cggatgaata 1320
caagctgctc aagaacgtcc gtggggacat caagttcctc aaggacgagc tggaggtcat 1380
gtgcgcgttc ctcctcaaga tgtcagatgt ggaggagcct gacgaaccaa caaagctgcg 1440
tgtgacggcg gtgcgggaga tgtcctataa gatagaggac aacatcgaca agttcatggt 1500
cctcgtggaa cacgagtcct cctgctccga ggcggctcat ggcgtcgcca agctcatgga 1560
caagtgcaag aacttgctgc cggacatcaa gacccgccgc aggatcgcca aggaggtcaa 1620
agacatcaag aaagaaatta aggatgtcag cgacagattt tcaaggtacc agatcttgtt 1680
ctctttacag tactattagt acctaccatc agatttgtga ccgatttgtt gccactcaag 1740
gtacaagatt gacgagtcct cctcctctat gccggcaaaa ggcaaggtcg accctcgact 1800
tcgtgcagtc tacaaagacg ccgcagagct cgtcggaatt gatggaccaa aggatgaact 1860
tgtcaagtgg ctgaatgaga aagagggcca atcactgaaa tctgtctcta ttgttggata 1920
tggagggttg ggcaagacca cgctcgccaa ccagatccgg gtcaaccttg gagcgacctt 1980
cgactgcggg gcttttgtct cgatttcacg caagcctgac atgaaggcga tattaagatc 2040
tatattatcg caaatcacca agaaagacga ggcttgctct agattagatg atatacaact 2100
catcatcgac aagattcgag aattcctcca agacacaagg tatgtatgtg agatctacta 2160
cgaagtaaca ttgcttaatt agctcatgca taatccgttg cctagaattt tgctaatgag 2220
attgctttgt gtgatttact ttcgtcctca acttgtatga ctgcgcttaa actttcaagt 2280
cattgtttct tgcaacttat gtcgatctgt tcactaatta agatatagct aaaatgtgtt 2340
tgccaatttc tgtcgccaca cattttctaa tttgattgcc acaggtttgt taaacttgcg 2400
gctatgttct gtttattaaa taattgtgac atggcgtcag tcaaaacaat aaagtacatg 2460
caattccctc tcttttgtac aagataaata tccgcacgca ctacatgact gcaaatatat 2520
ctatcttcta aggacccaaa gcacttgcat gtgcagacca ttgttaccgg atatgcagta 2580
cattaggttt gtgattagcc actttaagaa catagggttt ctagaggcta taaacctacg 2640
gttatcagta ttataatctt gaatggatat caaacatccg ttgaagaatc tatgcccata 2700
acacaaaaag agaaaaggga agaattaatc atagtcagat cggaccagcc tgactagcgt 2760
aacataatga caacagtagg ttgttgcgtc tttcctggtt tcgtagcaat ttgcatgacg 2820
aattgaggcg cagagccacc actcaatatg tggtcctacc aatcatgagg aaataacttg 2880
ttaccaaaat gacaaaagaa aataggtagt ccccatcatt gtttcaatat ctatcctctt 2940
gcgaacatgc tattggcagt ggcaaaacat tgtaccttgc cactggcaac agctgccttt 3000
actcggcctg agcattcata tttttttcct cttggttgtt aaatcttgct atttttcgct 3060
tgccttctat ggcaatcttt ttttttctta tattgccacc aaattgtatc taagagcaac 3120
tccaacacgc cgacgcaatt tttgtccatt tagatcggcc aagcggacgg ggtgtctggt 3180
tttgcctttg ggtggtgcgc ctacgcatta gtccgcgtgg gcatttaaaa aacttaaaaa 3240
gcttaaaata attaaatata aataaaaatg catattaaag cccgtcaaag tccacataat 3300
taaacataat acattaatat attaataaaa aacagccgcc ccagcccgca gccctaggcg 3360
tcttcgtcgg aggagtgggt gaggtcgaag aatgccggca gctcagccca cgggaaggcc 3420
tgctggtggg cagccagcac cgcctgctcc gctgtctgcg gcggcggcgg ccctacctgg 3480
caccgcgcac gcctcctcct cgcgctcctg ccgctcctcc tcctcatcgc acgcccaacg 3540
aagttttggc gctcctcccc ctcgggttcc cgctgcatgc ggacctggcc gtcgtaccgc 3600
tcctcctcca gcgtgcggtc cttccacgcc acgaggtagg ccgcctcctc ctccatcacg 3660
cacagccaaa tgggcggctt gctcatccgc ttgcagacga tgccctgcca ttggtaaacg 3720
ccggtgggcg cgggtggtgc tagcgctggt gcaggcggtg acgatgtggc tggtggtccc 3780
ggcaggtaga cactatagct ggcggccgat agctgcagcg tgtcatcgag gccagaccac 3840
taggagtcct cgcactaaga ctcctcggtg acgtgatggg ccccctcctc caatgccttg 3900
aggaacgccg cctcctcctc aacctactat ttgaccaccg gtggcggcgg tggagggcag 3960
gcttcgagct ggacggcgtc gcggaggcgc acatggtgct cgatggtgaa ccagacttct 4020
cagttagggg agtctggcct cgatgggtgg agtcgcgacg ctgctctggc gtcaggagag 4080
cacgccgggc ccgaatctcg ttggcgcgcg cacgctcgga ctgtggcacc gccggcactg 4140
gaatgtggtg ggggtcgagg tgccagccgt gggggaagtt cacgtccggc cacggcagtg 4200
agacgcggta ctcccggtgg tactacgcgt ggtgcaccgg gatgtactgg tggtcccgtg 4260
gcgctgccgc cctcccatcc cgcagcgccg acaccaatgc gaaccccttc gctgccggcc 4320
tcaccggccc ttgcaacgag ccggcctcgt ggtcgtgctt gccttgccct tacctttgcc 4380
aaagaaaccc atgacggcta gggttttctg tcaccggcga gggagcagat gtggactaga 4440
gtgggaggag aagtggatga ggcccgcccc gccgcaccct tgcaattaaa aagggcgtga 4500
tccggacgtt tctagtcact gccatgcggg cccgaggtag gtgggcgcca ttgactacgt 4560
ctgtggtggt agttggctgg cagacacgcc agcccgaggc gcacaccgag cggacgcgca 4620
acacgtccgt ctcgtgttcg tgtgaacgca aatcaggcac aaatttgggc tggaaatgcg 4680
tctacatgga tgccgaacgg acgtgatttg ggtttgtgtc cgcacgttgg gccggcgttt 4740
ttgtgccttt cgaccctaat ggacacacac ggaccaaatc cgttggcgtg ttagagttgc 4800
ttgtacctag tactactatg tgtggagttg ttatctttga gtttctcccc catctgtagc 4860
tgtgtattat tgtgagatgc tctcaggatt tcacttattt tagataatga caactttctt 4920
gatgctagac aattacatca aggtgtatta tatctaggat gcacacagat gtgagattgt 4980
ttcaggattt taggttactt ggtaggtgtt actataaata tctaatgagc tctaatgtgt 5040
atgttcatga aactgtgttg caggtacttt atcataattg atgatatatg ggagttagga 5100
acatgggaaa ctttgaaatg tgcatttgtc aagaatacat tgggcagcag aataattatc 5160
acaacacgta tagttgatgt tgccaaatct tgttctcctt ctagtgaaga tcttgtctat 5220
gagatgaaac cactcagtga ggctgactcg aagaaattgt ttttcaagag gatatttggc 5280
tgtgaagaaa gctgtcctga tagcttgaaa gaagctgcca atgatatttt gaaaaaatgc 5340
cgtggcctac ctctggccat caatgccatt tctagcgtgt tggtcacaac aagggaaaca 5400
aaagaagagt gggatcgggt gcgacattca atacgctctt caaaggtcaa aagtgatata 5460
atagagacca tgaattacat attgtctctt agttattttg atcttcccca ccacctaaga 5520
agctgcttat tgtacctggc tctgtttcct gaagaccaat tgattggaag gaagcggtta 5580
gtacgcagat ggatttctga aggctttatc catggagaaa gtggacaaga tctcatggaa 5640
ttaggagagg aatattttca ccagcttgtg aacagaagtc taatacagcc cggcaacata 5700
gggtatgacg gcaaggcaat gtattgccga gtccatgaca ccatccttga tttcctaatt 5760
gacaaatcct ctgaagagaa catgtgtact gtcctaaaga aacaatgcaa gcctaatggc 5820
atagttcgtc ggctctctct aatggggaat gaggatgaag aaatcgtgga gcaattggat 5880
ctgtcacacg ctcggtcaat cactgctttt ggggatatca agctattgcc ttcgcttggg 5940
aggtcaaaat gcttgcgcgt gctagacttg caagactgcg atcagttgga aaatcatcat 6000
atcaaggata ttgaaagact ctatcagcta agatacttgg atatctcttc tacaggaatc 6060
acggagcttc ccaggcaaat tggggagttg ctgtatctag agacgctagt tgcctatgga 6120
ttacgtgagc ttcctgaaag cacaagtcgg ctacaacgac tggcacgttt gtttgtttac 6180
tctggttgta agcttccagg tgggttggga aacttgataa acttgcaaga gcttgactgc 6240
gttgatgcct tgcatttgaa gcatgtggaa gagctcggca aactaacaaa tctgagaaag 6300
ctgagtatta aattggacac tggtgggatt gaaggcaaca aattagaaga gtccaaggaa 6360
aagctggtgt cctctctctg taaactggac gaatgcggcc tcctttccct gagtattgat 6420
tattatctga gagaaaagga tggagaagag ccgttcctcc ctgctttggg ttgtatccag 6480
gaggtttttg tatatggaca agatatcagc cggattagca gatggctcgc gtcacttccc 6540
aacctacacg ggttgctttt ggacaatccg aagatagagc agcaggatat tgagatgatt 6600
ggattgatac ccaatctgat cgatttgaca ctgtatttac gcataacaga cgacgctggg 6660
cggctcatca tcaagcgtga aggatttcaa cagttgcaga ggtttgagct ttcccgtatt 6720
cgtatggggg atttaatgtt tgaaccgggg gctatgccga ggctcaagga gcttatactc 6780
taccacttca tcgaaaagcc caaatctggt gcggtcgatt tcgactttgg catccaacac 6840
ctctccagcc ttgctcgcct cactgtcggc ttattatgcg ttggctcgac ggcggcggaa 6900
gtagaggctg cggaggatgc tttcaagagc atggccgagg cgaaccccaa ccgtccaata 6960
ctggagatga caagacttta tccgcagcat atgttacaag acgagcaaat aggtatgaca 7020
ggctcaggta ctattaacta gtttgtactt ttgtcttggt gagcagtaga tcaaaagatg 7080
tgcatatgct tcaatctttt ttccggtaat ctatttcagc tacaacgccg gccgtcaagt 7140
cctagatgcc taaaccgaga tgcgcctttg gttatggagc ccaagtcacg tactctgatt 7200
ggatgctgca gcaaatgaag ctacacattt ctattcgggc tacctctttt tttttgaaac 7260
tctattcggg ctacctcttt gtctcaaagc tggtgtactc ttgtgatttg tgttgcgcaa 7320
ctgatacttc tgtgtatgtc tgcaagtatc acgaattagt gggaatgact aggaataagt 7380
aagacaagta tcacgaattc agagtcacat gcaatttttt tcttttcttt tgggcataag 7440
tagattgcca tttgagtatt ttttccatta atatttgcgt attcttagat tgggacttag 7500
atatgtaacc cagatttctg ttgttaggaa acacataccg tgtagcttat caaaagaaaa 7560
agatctttag agaaaataac atcattagat tgttcaactt ggatgaaaaa tgtgaaaatg 7620
gccaaaccat aattaaataa catcattaga ttgttccctg caaaaaatac atcattagat 7680
tgttaaatat aattgaacct tttgttgtct tttttctaac atgcacgtgg cggtcaaaga 7740
tgcgtattaa aagactatgt cagcatgttt attgtgagtc ttggtatgaa tattggtctc 7800
tcctgattca tccattatgc ctggcaacat tttctataca aaaatgtaaa ttgaattaac 7860
atgcagacga ctgtcaaaga tatactactg tacatcaaag accacgtcaa tgcgtagaca 7920
gatttgtgtt tacaaactag gcgggtagtg ggaaaggatc ccatatgtgt tgaattgttg 7980
attatccact tttgctagga acggatctac atagtaaaag ccgacccccc aagttcgccc 8040
cagaagccgt tatttgaaat taaaat 8066
<210> 5
<211> 938
<212> PRT
<213> 栽培一粒小麦
<400> 5
Met Ala Glu Val Leu Val Ser Ala Ser Thr Gly Ala Met Gly Ser Leu
1 5 10 15
Leu Arg Lys Leu Gly Ala Met Leu Thr Asp Glu Tyr Lys Leu Leu Lys
20 25 30
Asn Val Arg Gly Asp Ile Lys Phe Leu Lys Asp Glu Leu Glu Val Met
35 40 45
Cys Ala Phe Leu Leu Lys Met Ser Asp Val Glu Glu Pro Asp Glu Pro
50 55 60
Thr Lys Leu Arg Val Thr Ala Val Arg Glu Met Ser Tyr Lys Ile Glu
65 70 75 80
Asp Asn Ile Asp Lys Phe Met Val Leu Val Glu His Glu Ser Ser Cys
85 90 95
Ser Glu Ala Ala His Gly Val Ala Lys Leu Met Asp Lys Cys Lys Asn
100 105 110
Leu Leu Pro Asp Ile Lys Thr Arg Arg Arg Ile Ala Lys Glu Val Lys
115 120 125
Asp Ile Lys Lys Glu Ile Lys Asp Val Ser Asp Arg Phe Ser Arg Tyr
130 135 140
Lys Ile Asp Glu Ser Ser Ser Ser Met Pro Ala Lys Gly Lys Val Asp
145 150 155 160
Pro Arg Leu Arg Ala Val Tyr Lys Asp Ala Ala Glu Leu Val Gly Ile
165 170 175
Asp Gly Pro Lys Asp Glu Leu Val Lys Trp Leu Asn Glu Lys Glu Gly
180 185 190
Gln Ser Leu Lys Ser Val Ser Ile Val Gly Tyr Gly Gly Leu Gly Lys
195 200 205
Thr Thr Leu Ala Asn Gln Ile Arg Val Asn Leu Gly Ala Thr Phe Asp
210 215 220
Cys Gly Ala Phe Val Ser Ile Ser Arg Lys Pro Asp Met Lys Ala Ile
225 230 235 240
Leu Arg Ser Ile Leu Ser Gln Ile Thr Lys Lys Asp Glu Ala Cys Ser
245 250 255
Arg Leu Asp Asp Ile Gln Leu Ile Ile Asp Lys Ile Arg Glu Phe Leu
260 265 270
Gln Asp Thr Arg Tyr Phe Ile Ile Ile Asp Asp Ile Trp Glu Leu Gly
275 280 285
Thr Trp Glu Thr Leu Lys Cys Ala Phe Val Lys Asn Thr Leu Gly Ser
290 295 300
Arg Ile Ile Ile Thr Thr Arg Ile Val Asp Val Ala Lys Ser Cys Ser
305 310 315 320
Pro Ser Ser Glu Asp Leu Val Tyr Glu Met Lys Pro Leu Ser Glu Ala
325 330 335
Asp Ser Lys Lys Leu Phe Phe Lys Arg Ile Phe Gly Cys Glu Glu Ser
340 345 350
Cys Pro Asp Ser Leu Lys Glu Ala Ala Asn Asp Ile Leu Lys Lys Cys
355 360 365
Arg Gly Leu Pro Leu Ala Ile Asn Ala Ile Ser Ser Val Leu Val Thr
370 375 380
Thr Arg Glu Thr Lys Glu Glu Trp Asp Arg Val Arg His Ser Ile Arg
385 390 395 400
Ser Ser Lys Val Lys Ser Asp Ile Ile Glu Thr Met Asn Tyr Ile Leu
405 410 415
Ser Leu Ser Tyr Phe Asp Leu Pro His His Leu Arg Ser Cys Leu Leu
420 425 430
Tyr Leu Ala Leu Phe Pro Glu Asp Gln Leu Ile Gly Arg Lys Arg Leu
435 440 445
Val Arg Arg Trp Ile Ser Glu Gly Phe Ile His Gly Glu Ser Gly Gln
450 455 460
Asp Leu Met Glu Leu Gly Glu Glu Tyr Phe His Gln Leu Val Asn Arg
465 470 475 480
Ser Leu Ile Gln Pro Gly Asn Ile Gly Tyr Asp Gly Lys Ala Met Tyr
485 490 495
Cys Arg Val His Asp Thr Ile Leu Asp Phe Leu Ile Asp Lys Ser Ser
500 505 510
Glu Glu Asn Met Cys Thr Val Leu Lys Lys Gln Cys Lys Pro Asn Gly
515 520 525
Ile Val Arg Arg Leu Ser Leu Met Gly Asn Glu Asp Glu Glu Ile Val
530 535 540
Glu Gln Leu Asp Leu Ser His Ala Arg Ser Ile Thr Ala Phe Gly Asp
545 550 555 560
Ile Lys Leu Leu Pro Ser Leu Gly Arg Ser Lys Cys Leu Arg Val Leu
565 570 575
Asp Leu Gln Asp Cys Asp Gln Leu Glu Asn His His Ile Lys Asp Ile
580 585 590
Glu Arg Leu Tyr Gln Leu Arg Tyr Leu Asp Ile Ser Ser Thr Gly Ile
595 600 605
Thr Glu Leu Pro Arg Gln Ile Gly Glu Leu Leu Tyr Leu Glu Thr Leu
610 615 620
Val Ala Tyr Gly Leu Arg Glu Leu Pro Glu Ser Thr Ser Arg Leu Gln
625 630 635 640
Arg Leu Ala Arg Leu Phe Val Tyr Ser Gly Cys Lys Leu Pro Gly Gly
645 650 655
Leu Gly Asn Leu Ile Asn Leu Gln Glu Leu Asp Cys Val Asp Ala Leu
660 665 670
His Leu Lys His Val Glu Glu Leu Gly Lys Leu Thr Asn Leu Arg Lys
675 680 685
Leu Ser Ile Lys Leu Asp Thr Gly Gly Ile Glu Gly Asn Lys Leu Glu
690 695 700
Glu Ser Lys Glu Lys Leu Val Ser Ser Leu Cys Lys Leu Asp Glu Cys
705 710 715 720
Gly Leu Leu Ser Leu Ser Ile Asp Tyr Tyr Leu Arg Glu Lys Asp Gly
725 730 735
Glu Glu Pro Phe Leu Pro Ala Leu Gly Cys Ile Gln Glu Val Phe Val
740 745 750
Tyr Gly Gln Asp Ile Ser Arg Ile Ser Arg Trp Leu Ala Ser Leu Pro
755 760 765
Asn Leu His Gly Leu Leu Leu Asp Asn Pro Lys Ile Glu Gln Gln Asp
770 775 780
Ile Glu Met Ile Gly Leu Ile Pro Asn Leu Ile Asp Leu Thr Leu Tyr
785 790 795 800
Leu Arg Ile Thr Asp Asp Ala Gly Arg Leu Ile Ile Lys Arg Glu Gly
805 810 815
Phe Gln Gln Leu Gln Arg Phe Glu Leu Ser Arg Ile Arg Met Gly Asp
820 825 830
Leu Met Phe Glu Pro Gly Ala Met Pro Arg Leu Lys Glu Leu Ile Leu
835 840 845
Tyr His Phe Ile Glu Lys Pro Lys Ser Gly Ala Val Asp Phe Asp Phe
850 855 860
Gly Ile Gln His Leu Ser Ser Leu Ala Arg Leu Thr Val Gly Leu Leu
865 870 875 880
Cys Val Gly Ser Thr Ala Ala Glu Val Glu Ala Ala Glu Asp Ala Phe
885 890 895
Lys Ser Met Ala Glu Ala Asn Pro Asn Arg Pro Ile Leu Glu Met Thr
900 905 910
Arg Leu Tyr Pro Gln His Met Leu Gln Asp Glu Gln Ile Gly Met Thr
915 920 925
Gly Ser Ala Thr Thr Pro Ala Val Lys Ser
930 935
<210> 6
<211> 2814
<212> DNA
<213> 栽培一粒小麦
<400> 6
atggcggaag ttctggtgag cgcatccaca ggggcaatgg gttccctcct gcggaagttg 60
ggagctatgt tgacggatga atacaagctg ctcaagaacg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgcg ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacacgagt cctcctgctc cgaggcggct 300
catggcgtcg ccaagctcat ggacaagtgc aagaacttgc tgccggacat caagacccgc 360
cgcaggatcg ccaaggaggt caaagacatc aagaaagaaa ttaaggatgt cagcgacaga 420
ttttcaaggt acaagattga cgagtcctcc tcctctatgc cggcaaaagg caaggtcgac 480
cctcgacttc gtgcagtcta caaagacgcc gcagagctcg tcggaattga tggaccaaag 540
gatgaacttg tcaagtggct gaatgagaaa gagggccaat cactgaaatc tgtctctatt 600
gttggatatg gagggttggg caagaccacg ctcgccaacc agatccgggt caaccttgga 660
gcgaccttcg actgcggggc ttttgtctcg atttcacgca agcctgacat gaaggcgata 720
ttaagatcta tattatcgca aatcaccaag aaagacgagg cttgctctag attagatgat 780
atacaactca tcatcgacaa gattcgagaa ttcctccaag acacaaggta ctttatcata 840
attgatgata tatgggagtt aggaacatgg gaaactttga aatgtgcatt tgtcaagaat 900
acattgggca gcagaataat tatcacaaca cgtatagttg atgttgccaa atcttgttct 960
ccttctagtg aagatcttgt ctatgagatg aaaccactca gtgaggctga ctcgaagaaa 1020
ttgtttttca agaggatatt tggctgtgaa gaaagctgtc ctgatagctt gaaagaagct 1080
gccaatgata ttttgaaaaa atgccgtggc ctacctctgg ccatcaatgc catttctagc 1140
gtgttggtca caacaaggga aacaaaagaa gagtgggatc gggtgcgaca ttcaatacgc 1200
tcttcaaagg tcaaaagtga tataatagag accatgaatt acatattgtc tcttagttat 1260
tttgatcttc cccaccacct aagaagctgc ttattgtacc tggctctgtt tcctgaagac 1320
caattgattg gaaggaagcg gttagtacgc agatggattt ctgaaggctt tatccatgga 1380
gaaagtggac aagatctcat ggaattagga gaggaatatt ttcaccagct tgtgaacaga 1440
agtctaatac agcccggcaa catagggtat gacggcaagg caatgtattg ccgagtccat 1500
gacaccatcc ttgatttcct aattgacaaa tcctctgaag agaacatgtg tactgtccta 1560
aagaaacaat gcaagcctaa tggcatagtt cgtcggctct ctctaatggg gaatgaggat 1620
gaagaaatcg tggagcaatt ggatctgtca cacgctcggt caatcactgc ttttggggat 1680
atcaagctat tgccttcgct tgggaggtca aaatgcttgc gcgtgctaga cttgcaagac 1740
tgcgatcagt tggaaaatca tcatatcaag gatattgaaa gactctatca gctaagatac 1800
ttggatatct cttctacagg aatcacggag cttcccaggc aaattgggga gttgctgtat 1860
ctagagacgc tagttgccta tggattacgt gagcttcctg aaagcacaag tcggctacaa 1920
cgactggcac gtttgtttgt ttactctggt tgtaagcttc caggtgggtt gggaaacttg 1980
ataaacttgc aagagcttga ctgcgttgat gccttgcatt tgaagcatgt ggaagagctc 2040
ggcaaactaa caaatctgag aaagctgagt attaaattgg acactggtgg gattgaaggc 2100
aacaaattag aagagtccaa ggaaaagctg gtgtcctctc tctgtaaact ggacgaatgc 2160
ggcctccttt ccctgagtat tgattattat ctgagagaaa aggatggaga agagccgttc 2220
ctccctgctt tgggttgtat ccaggaggtt tttgtatatg gacaagatat cagccggatt 2280
agcagatggc tcgcgtcact tcccaaccta cacgggttgc ttttggacaa tccgaagata 2340
gagcagcagg atattgagat gattggattg atacccaatc tgatcgattt gacactgtat 2400
ttacgcataa cagacgacgc tgggcggctc atcatcaagc gtgaaggatt tcaacagttg 2460
cagaggtttg agctttcccg tattcgtatg ggggatttaa tgtttgaacc gggggctatg 2520
ccgaggctca aggagcttat actctaccac ttcatcgaaa agcccaaatc tggtgcggtc 2580
gatttcgact ttggcatcca acacctctcc agccttgctc gcctcactgt cggcttatta 2640
tgcgttggct cgacggcggc ggaagtagag gctgcggagg atgctttcaa gagcatggcc 2700
gaggcgaacc ccaaccgtcc aatactggag atgacaagac tttatccgca gcatatgtta 2760
caagacgagc aaataggtat gacaggctca gctacaacgc cggccgtcaa gtcc 2814
<210> 7
<211> 5915
<212> DNA
<213> 栽培一粒小麦
<400> 7
atggcggaag ttctggtgag cgcatccaca ggggcaatgg gttccctcct gcggaagttg 60
ggagctatgt tgacggatga atacaagctg ctcaagaacg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgcg ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacaggaac acgggtcctc ctgttccgag 300
gcggctcatg gcgtcgccaa gctcatggac aagtgcaaga acttgctgcc ggacatcaag 360
gcccgccgca ggatcgccaa ggaggtcaaa gacatcaaga aagaaattaa ggatgtcagc 420
gacagatttt caaggtacca gatcttgttc tccttacagt actattagta cctaccatca 480
gatttgtgac cgatttgttg ccactcaagg tacaagattg acgactcctc ctcctctatg 540
ccggcaaaag acaaggtcga ccctcgactt cgtgcagtct acaaagacgc cgcagagctc 600
gtcggaattg atggaccaaa ggatgaactt gtcaagtggc tgaatgagaa agaaggccaa 660
tcactgaaat ctgtctctat tgttggatat ggagggttgg gcaagaccac gctcgccaac 720
cagatccggg tcaaccttgg agcgaccttc gactgcgggg cttttgtctc gatttcacgc 780
aagcctgaca tgaaggcgat attaagatct atattatcac aaatcaccaa gaaagacgat 840
gcttgctcta gattagatga tatacaactc atcatcgaca agattcgaga attcctccaa 900
gacacaaggt atgtatgtga gatctgctac gaagtaacat tgcttaatta gctcatgcat 960
aatcggttgc ctagaatttt gctaatgaag attgctttgt gtgatttact ttcgtcctca 1020
acttgtatga ctgcgcttaa actttcaagt cattgtttct tgcaacttat gtcgatctgt 1080
tcactaatta agatatagct aaaatgtgtt tgccgatttc tgtcgccaca cattttctaa 1140
tttgattgcc acaagtttgt taaacttgcg gctatgttct gtttattaaa taattgtgac 1200
atggcgtcag tcaaaacaat aaagtacatg caattccctc tcttttgtac aagataaata 1260
tccgcacgca ctacatgact gcaaatatat ctatcttcta aggacccaaa gcacttgcat 1320
gtgcagacca ttgttaccgg atatgcagta cattaggttt gtgattagcc actttaagaa 1380
catagggttt ctagaggcta taaacctacg gttatcagta ttataatctt gaatggatat 1440
caaacatccg ttgaagaatc tatgcccata acacaaaaag agaaaaggga agaattaatc 1500
atagtcagat cggaccagcc tgactagcgt aacataatga caacagtagg ttgttgcgtc 1560
tttcctggtt tcgtagcaat ttgcatgacg aattgaggcg cagagccacc actcaatatg 1620
tggtcctacc aatcatgagg aaataacttg ttaccaaaat gacaaaagaa aataggtagt 1680
ccccatcatt gtttcaatat ctatcctctt gcgaacatgc tattggcagt ggcaaaacat 1740
tgtaccttgc cactggcaac agctgccttt actcggcctg agcattcata tttttttcct 1800
cttggttgtt aaatcttgct atttttcgct tgccttctat ggcaatcttt ttttttctta 1860
tattgccacc aaattgtatc taagagcaac tccaacacgc cgacgcaatt tttgtccatt 1920
tagatcggcc aagcggacgg ggtgtctggt tttgcctttg ggtggtgcgc ctacgcatta 1980
gtccgcgtgg gcatttaaaa aacttaaaaa gcttaaaata attaaatata aataaaaatg 2040
catattaaag cccgtcaaag tccacataat taaacataat acattaatat attaataaaa 2100
aacagccgcc ccagcccgca gccctaggcg tcttcgtcgg aggagtgggt gaggtcgaag 2160
aatgccggca gctcagccca cgggaaggcc tgctggtggg cagccagcac cgcctgctcc 2220
gctgtctgcg gcggcggcgg ccctacctgg caccgcgcac gcctcctcct cgcgctcctg 2280
ccgctcctcc tcctcatcgc acgcccaacg aagttttggc gctcctcccc ctcgggttcc 2340
cgctgcatgc ggacctggcc gtcgtaccgc tcctcctcca gcgtgcggtc cttccacgcc 2400
acgaggtagg ccgcctcctc ctccatcacg cacagccaaa tgggcggctt gctcatccgc 2460
ttgcagacga tgccctgcca ttggtaaacg ccggtgggcg cgggtggtgc tagcgctggt 2520
gcaggcggtg acgatgtggc tggtggtccc ggcaggtaga cactatagct ggcggccgat 2580
agctgcagcg tgtcatcgag gccagaccac taggagtcct cgcactaaga ctcctcggtg 2640
acgtgatggg ccccctcctc caatgccttg aggaacgccg cctcctcctc aacctactat 2700
ttgaccaccg gtggcggcgg tggagggcag gcttcgagct ggacggcgtc gcggaggcgc 2760
acatggtgct cgatggtgaa ccagacttct cagttagggg agtctggcct cgatgggtgg 2820
agtcgcgacg ctgctctggc gtcaggagag cacgccgggc ccgaatctcg ttggcgcgcg 2880
cacgctcgga ctgtggcacc gccggcactg gaatgtggtg ggggtcgagg tgccagccgt 2940
gggggaagtt cacgtccggc cacggcagtg agacgcggta ctcccggtgg tactacgcgt 3000
ggtgcaccgg gatgtactgg tggtcccgtg gcgctgccgc cctcccatcc cgcagcgccg 3060
acaccaatgc gaaccccttc gctgccggcc tcaccggccc ttgcaacgag ccggcctcgt 3120
ggtcgtgctt gccttgccct tacctttgcc aaagaaaccc atgacggcta gggttttctg 3180
tcaccggcga gggagcagat gtggactaga gtgggaggag aagtggatga ggcccgcccc 3240
gccgcaccct tgcaattaaa aagggcgtga tccggacgtt tctagtcact gccatgcggg 3300
cccgaggtag gtgggcgcca ttgactacgt ctgtggtggt agttggctgg cagacacgcc 3360
agcccgaggc gcacaccgag cggacgcgca acacgtccgt ctcgtgttcg tgtgaacgca 3420
aatcaggcac aaatttgggc tggaaatgcg tctacatgga tgccgaacgg acgtgatttg 3480
ggtttgtgtc cgcacgttgg gccggcgttt ttgtgccttt cgaccctaat ggacacacac 3540
ggaccaaatc cgttggcgtg ttagagttgc ttgtacctag tactactatg tgtggagttg 3600
ttatctttga gtttctcccc catctgtagc tgtgtattat tgtgagatgc tctcaggatt 3660
tcacttattt tagataatga caactttctt gatgctagac aattacatca aggtgtatta 3720
tatctaggat gcacacagat gtgagattgt ttcaggattt taggttactt ggtaggtgtt 3780
actataaata tctaatgagc tctaatgtgt atgttcatga aactgtgttg caggtacttt 3840
atcataattg atgatatatg ggagttagga acatgggaaa ctttgaaatg tgcatttgtc 3900
aagaatacat tgggcagcag aataattatc acaacacgta tagttgatgt tgccaaatct 3960
tgttctcctt ctagtgaaga tcttgtctat gagatgaaac cactcagtga ggctgactcg 4020
aagaaattgt ttttcaagag gatatttggc tgtgaagaaa gctgtcctga tagcttgaaa 4080
gaagctgcca atgatatttt gaaaaaatgc cgtggcctac ctctggccat caatgccatt 4140
tctagcgtgt tggtcacaac aagggaaaca aaagaagagt gggatcgggt gcgacattca 4200
atacgctctt caaaggtcaa aagtgatata atagagacca tgaattacat attgtctctt 4260
agttattttg atcttcccca ccacctaaga agctgcttat tgtacctggc tctgtttcct 4320
gaagaccaat tgattggaag gaagcggtta gtacgcagat ggatttctga aggctttatc 4380
catggagaaa gtggacaaga tctcatggaa ttaggagagg aatattttca ccagcttgtg 4440
aacagaagtc taatacagcc cggcaacata gggtatgacg gcaaggcaat gtattgccga 4500
gtccatgaca ccatccttga tttcctaatt gacaaatcct ctgaagagaa catgtgtact 4560
gtcctaaaga aacaatgcaa gcctaatggc atagttcgtc ggctctctct aatggggaat 4620
gaggatgaag aaatcgtgga gcaattggat ctgtcacacg ctcggtcaat cactgctttt 4680
ggggatatca agctattgcc ttcgcttggg aggtcaaaat gcttgcgcgt gctagacttg 4740
caagactgcg atcagttgga aaatcatcat atcaaggata ttgaaagact ctatcagcta 4800
agatacttgg atatctcttc tacaggaatc acggagcttc ccaggcaaat tggggagttg 4860
ctgtatctag agacgctagt tgcctatgga ttacgtgagc ttcctgaaag cacaagtcgg 4920
ctacaacgac tggcacgttt gtttgtttac tctggttgta agcttccagg tgggttggga 4980
aacttgataa acttgcaaga gcttgactgc gttgatgcct tgcatttgaa gcatgtggaa 5040
gagctcggca aactaacaaa tctgagaaag ctgagtatta aattggacac tggtgggatt 5100
gaaggcaaca aattagaaga gtccaaggaa aagctggtgt cctctctctg taaactggac 5160
gaatgcggcc tcctttccct gagtattgat tattatctga gagaaaagga tggagaagag 5220
ccgttcctcc ctgctttggg ttgtatccag gaggtttttg tatatggaca agatatcagc 5280
cggattagca gatggctcgc gtcacttccc aacctacacg ggttgctttt ggactatccg 5340
aagatagagc agcaggatat tgagatgatt ggattgatac ccaatctgat cgatttgaca 5400
ctgtatttac gcataacaga cgacgctggg cggctcatca tcaagcgtga aggatttcaa 5460
cagttgcaga ggtttgagct ttcccgtatt cgtatggggg atttaatgtt tgaaccgggg 5520
gctatgccga ggctcaagga gcttatactc taccacttca tcgaaaagcc caaatctggt 5580
gcggtcgatt tcgactttgg catccaacac ctctccagcc ttgctcgcct cactgtcggc 5640
ttattatgcg ttggctcgac ggcggcggaa gtagaggctg cggaggatgc tttcaagagc 5700
atggccgagg cgaaccccaa ccgtccaata ctggagatga caagacttta tccgcagcat 5760
atgttacaag acgagcaaat aggtatgaca ggctcaggta ctattaacta gtttgtactt 5820
ttgtcttggt gagcagtaga tcaaaagatg tgcatatgct tcaatctttt ttccggtaat 5880
ctatttcagc tacaacgccg gccgtcaagt cctag 5915
<210> 8
<211> 940
<212> PRT
<213> 栽培一粒小麦
<400> 8
Met Ala Glu Val Leu Val Ser Ala Ser Thr Gly Ala Met Gly Ser Leu
1 5 10 15
Leu Arg Lys Leu Gly Ala Met Leu Thr Asp Glu Tyr Lys Leu Leu Lys
20 25 30
Asn Val Arg Gly Asp Ile Lys Phe Leu Lys Asp Glu Leu Glu Val Met
35 40 45
Cys Ala Phe Leu Leu Lys Met Ser Asp Val Glu Glu Pro Asp Glu Pro
50 55 60
Thr Lys Leu Arg Val Thr Ala Val Arg Glu Met Ser Tyr Lys Ile Glu
65 70 75 80
Asp Asn Ile Asp Lys Phe Met Val Leu Val Glu Gln Glu His Gly Ser
85 90 95
Ser Cys Ser Glu Ala Ala His Gly Val Ala Lys Leu Met Asp Lys Cys
100 105 110
Lys Asn Leu Leu Pro Asp Ile Lys Ala Arg Arg Arg Ile Ala Lys Glu
115 120 125
Val Lys Asp Ile Lys Lys Glu Ile Lys Asp Val Ser Asp Arg Phe Ser
130 135 140
Arg Tyr Lys Ile Asp Asp Ser Ser Ser Ser Met Pro Ala Lys Asp Lys
145 150 155 160
Val Asp Pro Arg Leu Arg Ala Val Tyr Lys Asp Ala Ala Glu Leu Val
165 170 175
Gly Ile Asp Gly Pro Lys Asp Glu Leu Val Lys Trp Leu Asn Glu Lys
180 185 190
Glu Gly Gln Ser Leu Lys Ser Val Ser Ile Val Gly Tyr Gly Gly Leu
195 200 205
Gly Lys Thr Thr Leu Ala Asn Gln Ile Arg Val Asn Leu Gly Ala Thr
210 215 220
Phe Asp Cys Gly Ala Phe Val Ser Ile Ser Arg Lys Pro Asp Met Lys
225 230 235 240
Ala Ile Leu Arg Ser Ile Leu Ser Gln Ile Thr Lys Lys Asp Asp Ala
245 250 255
Cys Ser Arg Leu Asp Asp Ile Gln Leu Ile Ile Asp Lys Ile Arg Glu
260 265 270
Phe Leu Gln Asp Thr Arg Tyr Phe Ile Ile Ile Asp Asp Ile Trp Glu
275 280 285
Leu Gly Thr Trp Glu Thr Leu Lys Cys Ala Phe Val Lys Asn Thr Leu
290 295 300
Gly Ser Arg Ile Ile Ile Thr Thr Arg Ile Val Asp Val Ala Lys Ser
305 310 315 320
Cys Ser Pro Ser Ser Glu Asp Leu Val Tyr Glu Met Lys Pro Leu Ser
325 330 335
Glu Ala Asp Ser Lys Lys Leu Phe Phe Lys Arg Ile Phe Gly Cys Glu
340 345 350
Glu Ser Cys Pro Asp Ser Leu Lys Glu Ala Ala Asn Asp Ile Leu Lys
355 360 365
Lys Cys Arg Gly Leu Pro Leu Ala Ile Asn Ala Ile Ser Ser Val Leu
370 375 380
Val Thr Thr Arg Glu Thr Lys Glu Glu Trp Asp Arg Val Arg His Ser
385 390 395 400
Ile Arg Ser Ser Lys Val Lys Ser Asp Ile Ile Glu Thr Met Asn Tyr
405 410 415
Ile Leu Ser Leu Ser Tyr Phe Asp Leu Pro His His Leu Arg Ser Cys
420 425 430
Leu Leu Tyr Leu Ala Leu Phe Pro Glu Asp Gln Leu Ile Gly Arg Lys
435 440 445
Arg Leu Val Arg Arg Trp Ile Ser Glu Gly Phe Ile His Gly Glu Ser
450 455 460
Gly Gln Asp Leu Met Glu Leu Gly Glu Glu Tyr Phe His Gln Leu Val
465 470 475 480
Asn Arg Ser Leu Ile Gln Pro Gly Asn Ile Gly Tyr Asp Gly Lys Ala
485 490 495
Met Tyr Cys Arg Val His Asp Thr Ile Leu Asp Phe Leu Ile Asp Lys
500 505 510
Ser Ser Glu Glu Asn Met Cys Thr Val Leu Lys Lys Gln Cys Lys Pro
515 520 525
Asn Gly Ile Val Arg Arg Leu Ser Leu Met Gly Asn Glu Asp Glu Glu
530 535 540
Ile Val Glu Gln Leu Asp Leu Ser His Ala Arg Ser Ile Thr Ala Phe
545 550 555 560
Gly Asp Ile Lys Leu Leu Pro Ser Leu Gly Arg Ser Lys Cys Leu Arg
565 570 575
Val Leu Asp Leu Gln Asp Cys Asp Gln Leu Glu Asn His His Ile Lys
580 585 590
Asp Ile Glu Arg Leu Tyr Gln Leu Arg Tyr Leu Asp Ile Ser Ser Thr
595 600 605
Gly Ile Thr Glu Leu Pro Arg Gln Ile Gly Glu Leu Leu Tyr Leu Glu
610 615 620
Thr Leu Val Ala Tyr Gly Leu Arg Glu Leu Pro Glu Ser Thr Ser Arg
625 630 635 640
Leu Gln Arg Leu Ala Arg Leu Phe Val Tyr Ser Gly Cys Lys Leu Pro
645 650 655
Gly Gly Leu Gly Asn Leu Ile Asn Leu Gln Glu Leu Asp Cys Val Asp
660 665 670
Ala Leu His Leu Lys His Val Glu Glu Leu Gly Lys Leu Thr Asn Leu
675 680 685
Arg Lys Leu Ser Ile Lys Leu Asp Thr Gly Gly Ile Glu Gly Asn Lys
690 695 700
Leu Glu Glu Ser Lys Glu Lys Leu Val Ser Ser Leu Cys Lys Leu Asp
705 710 715 720
Glu Cys Gly Leu Leu Ser Leu Ser Ile Asp Tyr Tyr Leu Arg Glu Lys
725 730 735
Asp Gly Glu Glu Pro Phe Leu Pro Ala Leu Gly Cys Ile Gln Glu Val
740 745 750
Phe Val Tyr Gly Gln Asp Ile Ser Arg Ile Ser Arg Trp Leu Ala Ser
755 760 765
Leu Pro Asn Leu His Gly Leu Leu Leu Asp Tyr Pro Lys Ile Glu Gln
770 775 780
Gln Asp Ile Glu Met Ile Gly Leu Ile Pro Asn Leu Ile Asp Leu Thr
785 790 795 800
Leu Tyr Leu Arg Ile Thr Asp Asp Ala Gly Arg Leu Ile Ile Lys Arg
805 810 815
Glu Gly Phe Gln Gln Leu Gln Arg Phe Glu Leu Ser Arg Ile Arg Met
820 825 830
Gly Asp Leu Met Phe Glu Pro Gly Ala Met Pro Arg Leu Lys Glu Leu
835 840 845
Ile Leu Tyr His Phe Ile Glu Lys Pro Lys Ser Gly Ala Val Asp Phe
850 855 860
Asp Phe Gly Ile Gln His Leu Ser Ser Leu Ala Arg Leu Thr Val Gly
865 870 875 880
Leu Leu Cys Val Gly Ser Thr Ala Ala Glu Val Glu Ala Ala Glu Asp
885 890 895
Ala Phe Lys Ser Met Ala Glu Ala Asn Pro Asn Arg Pro Ile Leu Glu
900 905 910
Met Thr Arg Leu Tyr Pro Gln His Met Leu Gln Asp Glu Gln Ile Gly
915 920 925
Met Thr Gly Ser Ala Thr Thr Pro Ala Val Lys Ser
930 935 940
<210> 9
<211> 2820
<212> DNA
<213> 栽培一粒小麦
<400> 9
atggcggaag ttctggtgag cgcatccaca ggggcaatgg gttccctcct gcggaagttg 60
ggagctatgt tgacggatga atacaagctg ctcaagaacg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgcg ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacaggaac acgggtcctc ctgttccgag 300
gcggctcatg gcgtcgccaa gctcatggac aagtgcaaga acttgctgcc ggacatcaag 360
gcccgccgca ggatcgccaa ggaggtcaaa gacatcaaga aagaaattaa ggatgtcagc 420
gacagatttt caaggtacaa gattgacgac tcctcctcct ctatgccggc aaaagacaag 480
gtcgaccctc gacttcgtgc agtctacaaa gacgccgcag agctcgtcgg aattgatgga 540
ccaaaggatg aacttgtcaa gtggctgaat gagaaagaag gccaatcact gaaatctgtc 600
tctattgttg gatatggagg gttgggcaag accacgctcg ccaaccagat ccgggtcaac 660
cttggagcga ccttcgactg cggggctttt gtctcgattt cacgcaagcc tgacatgaag 720
gcgatattaa gatctatatt atcacaaatc accaagaaag acgatgcttg ctctagatta 780
gatgatatac aactcatcat cgacaagatt cgagaattcc tccaagacac aaggtacttt 840
atcataattg atgatatatg ggagttagga acatgggaaa ctttgaaatg tgcatttgtc 900
aagaatacat tgggcagcag aataattatc acaacacgta tagttgatgt tgccaaatct 960
tgttctcctt ctagtgaaga tcttgtctat gagatgaaac cactcagtga ggctgactcg 1020
aagaaattgt ttttcaagag gatatttggc tgtgaagaaa gctgtcctga tagcttgaaa 1080
gaagctgcca atgatatttt gaaaaaatgc cgtggcctac ctctggccat caatgccatt 1140
tctagcgtgt tggtcacaac aagggaaaca aaagaagagt gggatcgggt gcgacattca 1200
atacgctctt caaaggtcaa aagtgatata atagagacca tgaattacat attgtctctt 1260
agttattttg atcttcccca ccacctaaga agctgcttat tgtacctggc tctgtttcct 1320
gaagaccaat tgattggaag gaagcggtta gtacgcagat ggatttctga aggctttatc 1380
catggagaaa gtggacaaga tctcatggaa ttaggagagg aatattttca ccagcttgtg 1440
aacagaagtc taatacagcc cggcaacata gggtatgacg gcaaggcaat gtattgccga 1500
gtccatgaca ccatccttga tttcctaatt gacaaatcct ctgaagagaa catgtgtact 1560
gtcctaaaga aacaatgcaa gcctaatggc atagttcgtc ggctctctct aatggggaat 1620
gaggatgaag aaatcgtgga gcaattggat ctgtcacacg ctcggtcaat cactgctttt 1680
ggggatatca agctattgcc ttcgcttggg aggtcaaaat gcttgcgcgt gctagacttg 1740
caagactgcg atcagttgga aaatcatcat atcaaggata ttgaaagact ctatcagcta 1800
agatacttgg atatctcttc tacaggaatc acggagcttc ccaggcaaat tggggagttg 1860
ctgtatctag agacgctagt tgcctatgga ttacgtgagc ttcctgaaag cacaagtcgg 1920
ctacaacgac tggcacgttt gtttgtttac tctggttgta agcttccagg tgggttggga 1980
aacttgataa acttgcaaga gcttgactgc gttgatgcct tgcatttgaa gcatgtggaa 2040
gagctcggca aactaacaaa tctgagaaag ctgagtatta aattggacac tggtgggatt 2100
gaaggcaaca aattagaaga gtccaaggaa aagctggtgt cctctctctg taaactggac 2160
gaatgcggcc tcctttccct gagtattgat tattatctga gagaaaagga tggagaagag 2220
ccgttcctcc ctgctttggg ttgtatccag gaggtttttg tatatggaca agatatcagc 2280
cggattagca gatggctcgc gtcacttccc aacctacacg ggttgctttt ggactatccg 2340
aagatagagc agcaggatat tgagatgatt ggattgatac ccaatctgat cgatttgaca 2400
ctgtatttac gcataacaga cgacgctggg cggctcatca tcaagcgtga aggatttcaa 2460
cagttgcaga ggtttgagct ttcccgtatt cgtatggggg atttaatgtt tgaaccgggg 2520
gctatgccga ggctcaagga gcttatactc taccacttca tcgaaaagcc caaatctggt 2580
gcggtcgatt tcgactttgg catccaacac ctctccagcc ttgctcgcct cactgtcggc 2640
ttattatgcg ttggctcgac ggcggcggaa gtagaggctg cggaggatgc tttcaagagc 2700
atggccgagg cgaaccccaa ccgtccaata ctggagatga caagacttta tccgcagcat 2760
atgttacaag acgagcaaat aggtatgaca ggctcagcta caacgccggc cgtcaagtcc 2820
<210> 10
<211> 5915
<212> DNA
<213> 栽培一粒小麦
<400> 10
atggcggaag ttctggtgag cgcatccaca ggggcaatgg gttccctcct gcggaagttg 60
ggagctatgt tgacggatga atacaagctg ctcaagaacg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgcg ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacaggaac acgggtcctc ctgttccgag 300
gcggctcatg gcgtcgccaa gctcatggac aagtgcaaga acttgctgcc ggacatcaag 360
gcccgccgca ggatcgccaa ggaggtcaaa gacatcaaga aagaaattaa ggatgtcagc 420
gacagatttt caaggtacca gatcttgttc tccttacagt actattagta cctaccatca 480
gatttgtgac cgatttgttg ccactcaagg tacaagattg acgactcctc ctcctctatg 540
ccggcaaaag acaaggtcga ccctcgactt cgtgcagtct acaaagacgc cgcagagctc 600
gtcggaattg atggaccaaa ggatgaactt gtcaagtggc tgaatgagaa agaaggccaa 660
tcactgaaat ctgtctctat tgttggatat ggagggttgg gcaagaccac gctcgccaac 720
cagatccggg tcaaccttgg agcgaccttc gactgcgggg cttttgtctc gatttcacgc 780
aagcctgaca tgaaggcgat attaagatct atattatcac aaatcaccaa gaaagacgat 840
gcttgctcta gattagatga tatacaactc atcatcgaca agattcgaga attcctccaa 900
gacacaaggt atgtatgtga gatctgctac gaagtaacat tgcttaatta gctcatgcat 960
aatcggttgc ctagaatttt gctaatgaag attgctttgt gtgatttact ttcgtcctca 1020
acttgtatga ctgcgcttaa actttcaagt cattgtttct tgcaacttat gtcgatctgt 1080
tcactaatta agatatagct aaaatgtgtt tgccgatttc tgtcgccaca cattttctaa 1140
tttgattgcc acaagtttgt taaacttgcg gctatgttct gtttattaaa taattgtgac 1200
atggcgtcag tcaaaacaat aaagtacatg caattccctc tcttttgtac aagataaata 1260
tccgcacgca ctacatgact gcaaatatat ctatcttcta aggacccaaa gcacttgcat 1320
gtgcagacca ttgttaccgg atatgcagta cattaggttt gtgattagcc actttaagaa 1380
catagggttt ctagaggcta taaacctacg gttatcagta ttataatctt gaatggatat 1440
caaacatccg ttgaagaatc tatgcccata acacaaaaag agaaaaggga agaattaatc 1500
atagtcagat cggaccagcc tgactagcgt aacataatga caacagtagg ttgttgcgtc 1560
tttcctggtt tcgtagcaat ttgcatgacg aattgaggcg cagagccacc actcaatatg 1620
tggtcctacc aatcatgagg aaataacttg ttaccaaaat gacaaaagaa aataggtagt 1680
ccccatcatt gtttcaatat ctatcctctt gcgaacatgc tattggcagt ggcaaaacat 1740
tgtaccttgc cactggcaac agctgccttt actcggcctg agcattcata tttttttcct 1800
cttggttgtt aaatcttgct atttttcgct tgccttctat ggcaatcttt ttttttctta 1860
tattgccacc aaattgtatc taagagcaac tccaacacgc cgacgcaatt tttgtccatt 1920
tagatcggcc aagcggacgg ggtgtctggt tttgcctttg ggtggtgcgc ctacgcatta 1980
gtccgcgtgg gcatttaaaa aacttaaaaa gcttaaaata attaaatata aataaaaatg 2040
catattaaag cccgtcaaag tccacataat taaacataat acattaatat attaataaaa 2100
aacagccgcc ccagcccgca gccctaggcg tcttcgtcgg aggagtgggt gaggtcgaag 2160
aatgccggca gctcagccca cgggaaggcc tgctggtggg cagccagcac cgcctgctcc 2220
gctgtctgcg gcggcggcgg ccctacctgg caccgcgcac gcctcctcct cgcgctcctg 2280
ccgctcctcc tcctcatcgc acgcccaacg aagttttggc gctcctcccc ctcgggttcc 2340
cgctgcatgc ggacctggcc gtcgtaccgc tcctcctcca gcgtgcggtc cttccacgcc 2400
acgaggtagg ccgcctcctc ctccatcacg cacagccaaa tgggcggctt gctcatccgc 2460
ttgcagacga tgccctgcca ttggtaaacg ccggtgggcg cgggtggtgc tagcgctggt 2520
gcaggcggtg acgatgtggc tggtggtccc ggcaggtaga cactatagct ggcggccgat 2580
agctgcagcg tgtcatcgag gccagaccac taggagtcct cgcactaaga ctcctcggtg 2640
acgtgatggg ccccctcctc caatgccttg aggaacgccg cctcctcctc aacctactat 2700
ttgaccaccg gtggcggcgg tggagggcag gcttcgagct ggacggcgtc gcggaggcgc 2760
acatggtgct cgatggtgaa ccagacttct cagttagggg agtctggcct cgatgggtgg 2820
agtcgcgacg ctgctctggc gtcaggagag cacgccgggc ccgaatctcg ttggcgcgcg 2880
cacgctcgga ctgtggcacc gccggcactg gaatgtggtg ggggtcgagg tgccagccgt 2940
gggggaagtt cacgtccggc cacggcagtg agacgcggta ctcccggtgg tactacgcgt 3000
ggtgcaccgg gatgtactgg tggtcccgtg gcgctgccgc cctcccatcc cgcagcgccg 3060
acaccaatgc gaaccccttc gctgccggcc tcaccggccc ttgcaacgag ccggcctcgt 3120
ggtcgtgctt gccttgccct tacctttgcc aaagaaaccc atgacggcta gggttttctg 3180
tcaccggcga gggagcagat gtggactaga gtgggaggag aagtggatga ggcccgcccc 3240
gccgcaccct tgcaattaaa aagggcgtga tccggacgtt tctagtcact gccatgcggg 3300
cccgaggtag gtgggcgcca ttgactacgt ctgtggtggt agttggctgg cagacacgcc 3360
agcccgaggc gcacaccgag cggacgcgca acacgtccgt ctcgtgttcg tgtgaacgca 3420
aatcaggcac aaatttgggc tggaaatgcg tctacatgga tgccgaacgg acgtgatttg 3480
ggtttgtgtc cgcacgttgg gccggcgttt ttgtgccttt cgaccctaat ggacacacac 3540
ggaccaaatc cgttggcgtg ttagagttgc ttgtacctag tactactatg tgtggagttg 3600
ttatctttga gtttctcccc catctgtagc tgtgtattat tgtgagatgc tctcaggatt 3660
tcacttattt tagataatga caactttctt gatgctagac aattacatca aggtgtatta 3720
tatctaggat gcacacagat gtgagattgt ttcaggattt taggttactt ggtaggtgtt 3780
actataaata tctaatgagc tctaatgtgt atgttcatga aactgtgttg caggtacttt 3840
atcataattg atgatatatg ggagttagga acatgggaaa ctttgaaatg tgcatttgtc 3900
aagaatacat tgggcagcag aataattatc acaacacgta tagttgatgt tgccaaatct 3960
tgttctcctt ctagtgaaga tcttgtctat gagatgaaac cactcagtga ggctgactcg 4020
aagaaattgt ttttcaagag gatatttggc tgtgaagaaa gctgtcctga tagcttgaaa 4080
gaagctgcca atgatatttt gaaaaaatgc cgtggcctac ctctggccat caatgccatt 4140
tctagcgtgt tggtcacaac aagggaaaca aaagaagagt gggatcgggt gcgacattca 4200
atacgctctt caaaggtcaa aagtgatata atagagacca tgaattacat attgtctctt 4260
agttattttg atcttcccca ccacctaaga agctgcttat tgtacctggc tctgtttcct 4320
gaagaccaat tgattggaag gaagcggtta gtacgcagat ggatttctga aggctttatc 4380
catggagaaa gtggacaaga tctcatggaa ttaggagagg aatattttca ccagcttgtg 4440
aacagaagtc taatacagcc cggcaacata gggtatgacg gcaaggcaat gtattgccga 4500
gtccatgaca ccatccttga tttcctaatt gacaaatcct ctgaagagaa catgtgtact 4560
gtcctaaaga aacaatgcaa gcctaatggc atagttcgtc ggctctctct aatggggaat 4620
gaggatgaag aaatcgtgga gcaattggat ctgtcacacg ctcggtcaat cactgctttt 4680
ggggatatca agctattgcc ttcgcttggg aggtcaaaat gcttgcgcgt gctagacttg 4740
caagactgcg atcagttgga aaatcatcat atcaaggata ttgaaagact ctatcagcta 4800
agatacttgg atatctcttc tacaggaatc acggagcttc ccaggcaaat tggggagttg 4860
ctgtatctag agacgctagt tgcctatgga ttacgtgagc ttcctgaaag cacaagtcgg 4920
ctacaacgac tggcacgttt gtttgtttac tctggttgta agcttccagg tgggttggga 4980
aacttgataa acttgcaaga gcttgactgc gttgatgcct tgcatttgaa gcatgtggaa 5040
gagctcggca aactaacaaa tctgagaaag ctgagtatta aattggacac tggtgggatt 5100
gaaggcaaca aattagaaga gtccaaggaa aagctggtgt cctctctctg taaactggac 5160
gaatgcggcc tcctttccct gagtattgat tattatctga gagaaaagga tggagaagag 5220
ccgttcctcc ctgctttggg ttgtatccag gaggtttttg tatatggaca agatatcagc 5280
cggattagca gatggctcgc gtcacttccc aacctacacg ggttgctttt ggacaatccg 5340
aagatagagc agcaggatat tgagatgatt ggattgatac ccaatctgat cgatttgaca 5400
ctgtatttac gcataacaga cgacgctggg cggctcatca tcaagcgtga aggatttcaa 5460
cagttgcaga ggtttgagct ttcccgtatt cgtatggggg atttaatgtt tgaaccgggg 5520
gctatgccga ggctcaagga gcttatactc taccacttca tcgaaaagcc caaatctggt 5580
gcggtcgatt tcgactttgg catccaacac ctctccagcc ttgctcgcct cactgtcggc 5640
ttattatgcg ttggctcgac ggcggcggaa gtagaggctg cggaggatgc tttcaagagc 5700
atggccgagg cgaaccccaa ccgtccaata ctggagatga caagacttta tccgcagcat 5760
atgttacaag acgagcaaat aggtatgaca ggctcaggta ctattaacta gtttgtactt 5820
ttgtcttggt gagcagtaga tcaaaagatg tgcatatgct tcaatctttt ttccggtaat 5880
ctatttcagc tacaacgccg gccgtcaagt cctag 5915
<210> 11
<211> 940
<212> PRT
<213> 栽培一粒小麦
<400> 11
Met Ala Glu Val Leu Val Ser Ala Ser Thr Gly Ala Met Gly Ser Leu
1 5 10 15
Leu Arg Lys Leu Gly Ala Met Leu Thr Asp Glu Tyr Lys Leu Leu Lys
20 25 30
Asn Val Arg Gly Asp Ile Lys Phe Leu Lys Asp Glu Leu Glu Val Met
35 40 45
Cys Ala Phe Leu Leu Lys Met Ser Asp Val Glu Glu Pro Asp Glu Pro
50 55 60
Thr Lys Leu Arg Val Thr Ala Val Arg Glu Met Ser Tyr Lys Ile Glu
65 70 75 80
Asp Asn Ile Asp Lys Phe Met Val Leu Val Glu Gln Glu His Gly Ser
85 90 95
Ser Cys Ser Glu Ala Ala His Gly Val Ala Lys Leu Met Asp Lys Cys
100 105 110
Lys Asn Leu Leu Pro Asp Ile Lys Ala Arg Arg Arg Ile Ala Lys Glu
115 120 125
Val Lys Asp Ile Lys Lys Glu Ile Lys Asp Val Ser Asp Arg Phe Ser
130 135 140
Arg Tyr Lys Ile Asp Asp Ser Ser Ser Ser Met Pro Ala Lys Asp Lys
145 150 155 160
Val Asp Pro Arg Leu Arg Ala Val Tyr Lys Asp Ala Ala Glu Leu Val
165 170 175
Gly Ile Asp Gly Pro Lys Asp Glu Leu Val Lys Trp Leu Asn Glu Lys
180 185 190
Glu Gly Gln Ser Leu Lys Ser Val Ser Ile Val Gly Tyr Gly Gly Leu
195 200 205
Gly Lys Thr Thr Leu Ala Asn Gln Ile Arg Val Asn Leu Gly Ala Thr
210 215 220
Phe Asp Cys Gly Ala Phe Val Ser Ile Ser Arg Lys Pro Asp Met Lys
225 230 235 240
Ala Ile Leu Arg Ser Ile Leu Ser Gln Ile Thr Lys Lys Asp Asp Ala
245 250 255
Cys Ser Arg Leu Asp Asp Ile Gln Leu Ile Ile Asp Lys Ile Arg Glu
260 265 270
Phe Leu Gln Asp Thr Arg Tyr Phe Ile Ile Ile Asp Asp Ile Trp Glu
275 280 285
Leu Gly Thr Trp Glu Thr Leu Lys Cys Ala Phe Val Lys Asn Thr Leu
290 295 300
Gly Ser Arg Ile Ile Ile Thr Thr Arg Ile Val Asp Val Ala Lys Ser
305 310 315 320
Cys Ser Pro Ser Ser Glu Asp Leu Val Tyr Glu Met Lys Pro Leu Ser
325 330 335
Glu Ala Asp Ser Lys Lys Leu Phe Phe Lys Arg Ile Phe Gly Cys Glu
340 345 350
Glu Ser Cys Pro Asp Ser Leu Lys Glu Ala Ala Asn Asp Ile Leu Lys
355 360 365
Lys Cys Arg Gly Leu Pro Leu Ala Ile Asn Ala Ile Ser Ser Val Leu
370 375 380
Val Thr Thr Arg Glu Thr Lys Glu Glu Trp Asp Arg Val Arg His Ser
385 390 395 400
Ile Arg Ser Ser Lys Val Lys Ser Asp Ile Ile Glu Thr Met Asn Tyr
405 410 415
Ile Leu Ser Leu Ser Tyr Phe Asp Leu Pro His His Leu Arg Ser Cys
420 425 430
Leu Leu Tyr Leu Ala Leu Phe Pro Glu Asp Gln Leu Ile Gly Arg Lys
435 440 445
Arg Leu Val Arg Arg Trp Ile Ser Glu Gly Phe Ile His Gly Glu Ser
450 455 460
Gly Gln Asp Leu Met Glu Leu Gly Glu Glu Tyr Phe His Gln Leu Val
465 470 475 480
Asn Arg Ser Leu Ile Gln Pro Gly Asn Ile Gly Tyr Asp Gly Lys Ala
485 490 495
Met Tyr Cys Arg Val His Asp Thr Ile Leu Asp Phe Leu Ile Asp Lys
500 505 510
Ser Ser Glu Glu Asn Met Cys Thr Val Leu Lys Lys Gln Cys Lys Pro
515 520 525
Asn Gly Ile Val Arg Arg Leu Ser Leu Met Gly Asn Glu Asp Glu Glu
530 535 540
Ile Val Glu Gln Leu Asp Leu Ser His Ala Arg Ser Ile Thr Ala Phe
545 550 555 560
Gly Asp Ile Lys Leu Leu Pro Ser Leu Gly Arg Ser Lys Cys Leu Arg
565 570 575
Val Leu Asp Leu Gln Asp Cys Asp Gln Leu Glu Asn His His Ile Lys
580 585 590
Asp Ile Glu Arg Leu Tyr Gln Leu Arg Tyr Leu Asp Ile Ser Ser Thr
595 600 605
Gly Ile Thr Glu Leu Pro Arg Gln Ile Gly Glu Leu Leu Tyr Leu Glu
610 615 620
Thr Leu Val Ala Tyr Gly Leu Arg Glu Leu Pro Glu Ser Thr Ser Arg
625 630 635 640
Leu Gln Arg Leu Ala Arg Leu Phe Val Tyr Ser Gly Cys Lys Leu Pro
645 650 655
Gly Gly Leu Gly Asn Leu Ile Asn Leu Gln Glu Leu Asp Cys Val Asp
660 665 670
Ala Leu His Leu Lys His Val Glu Glu Leu Gly Lys Leu Thr Asn Leu
675 680 685
Arg Lys Leu Ser Ile Lys Leu Asp Thr Gly Gly Ile Glu Gly Asn Lys
690 695 700
Leu Glu Glu Ser Lys Glu Lys Leu Val Ser Ser Leu Cys Lys Leu Asp
705 710 715 720
Glu Cys Gly Leu Leu Ser Leu Ser Ile Asp Tyr Tyr Leu Arg Glu Lys
725 730 735
Asp Gly Glu Glu Pro Phe Leu Pro Ala Leu Gly Cys Ile Gln Glu Val
740 745 750
Phe Val Tyr Gly Gln Asp Ile Ser Arg Ile Ser Arg Trp Leu Ala Ser
755 760 765
Leu Pro Asn Leu His Gly Leu Leu Leu Asp Asn Pro Lys Ile Glu Gln
770 775 780
Gln Asp Ile Glu Met Ile Gly Leu Ile Pro Asn Leu Ile Asp Leu Thr
785 790 795 800
Leu Tyr Leu Arg Ile Thr Asp Asp Ala Gly Arg Leu Ile Ile Lys Arg
805 810 815
Glu Gly Phe Gln Gln Leu Gln Arg Phe Glu Leu Ser Arg Ile Arg Met
820 825 830
Gly Asp Leu Met Phe Glu Pro Gly Ala Met Pro Arg Leu Lys Glu Leu
835 840 845
Ile Leu Tyr His Phe Ile Glu Lys Pro Lys Ser Gly Ala Val Asp Phe
850 855 860
Asp Phe Gly Ile Gln His Leu Ser Ser Leu Ala Arg Leu Thr Val Gly
865 870 875 880
Leu Leu Cys Val Gly Ser Thr Ala Ala Glu Val Glu Ala Ala Glu Asp
885 890 895
Ala Phe Lys Ser Met Ala Glu Ala Asn Pro Asn Arg Pro Ile Leu Glu
900 905 910
Met Thr Arg Leu Tyr Pro Gln His Met Leu Gln Asp Glu Gln Ile Gly
915 920 925
Met Thr Gly Ser Ala Thr Thr Pro Ala Val Lys Ser
930 935 940
<210> 12
<211> 2820
<212> DNA
<213> 栽培一粒小麦
<400> 12
atggcggaag ttctggtgag cgcatccaca ggggcaatgg gttccctcct gcggaagttg 60
ggagctatgt tgacggatga atacaagctg ctcaagaacg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgcg ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacaggaac acgggtcctc ctgttccgag 300
gcggctcatg gcgtcgccaa gctcatggac aagtgcaaga acttgctgcc ggacatcaag 360
gcccgccgca ggatcgccaa ggaggtcaaa gacatcaaga aagaaattaa ggatgtcagc 420
gacagatttt caaggtacaa gattgacgac tcctcctcct ctatgccggc aaaagacaag 480
gtcgaccctc gacttcgtgc agtctacaaa gacgccgcag agctcgtcgg aattgatgga 540
ccaaaggatg aacttgtcaa gtggctgaat gagaaagaag gccaatcact gaaatctgtc 600
tctattgttg gatatggagg gttgggcaag accacgctcg ccaaccagat ccgggtcaac 660
cttggagcga ccttcgactg cggggctttt gtctcgattt cacgcaagcc tgacatgaag 720
gcgatattaa gatctatatt atcacaaatc accaagaaag acgatgcttg ctctagatta 780
gatgatatac aactcatcat cgacaagatt cgagaattcc tccaagacac aaggtacttt 840
atcataattg atgatatatg ggagttagga acatgggaaa ctttgaaatg tgcatttgtc 900
aagaatacat tgggcagcag aataattatc acaacacgta tagttgatgt tgccaaatct 960
tgttctcctt ctagtgaaga tcttgtctat gagatgaaac cactcagtga ggctgactcg 1020
aagaaattgt ttttcaagag gatatttggc tgtgaagaaa gctgtcctga tagcttgaaa 1080
gaagctgcca atgatatttt gaaaaaatgc cgtggcctac ctctggccat caatgccatt 1140
tctagcgtgt tggtcacaac aagggaaaca aaagaagagt gggatcgggt gcgacattca 1200
atacgctctt caaaggtcaa aagtgatata atagagacca tgaattacat attgtctctt 1260
agttattttg atcttcccca ccacctaaga agctgcttat tgtacctggc tctgtttcct 1320
gaagaccaat tgattggaag gaagcggtta gtacgcagat ggatttctga aggctttatc 1380
catggagaaa gtggacaaga tctcatggaa ttaggagagg aatattttca ccagcttgtg 1440
aacagaagtc taatacagcc cggcaacata gggtatgacg gcaaggcaat gtattgccga 1500
gtccatgaca ccatccttga tttcctaatt gacaaatcct ctgaagagaa catgtgtact 1560
gtcctaaaga aacaatgcaa gcctaatggc atagttcgtc ggctctctct aatggggaat 1620
gaggatgaag aaatcgtgga gcaattggat ctgtcacacg ctcggtcaat cactgctttt 1680
ggggatatca agctattgcc ttcgcttggg aggtcaaaat gcttgcgcgt gctagacttg 1740
caagactgcg atcagttgga aaatcatcat atcaaggata ttgaaagact ctatcagcta 1800
agatacttgg atatctcttc tacaggaatc acggagcttc ccaggcaaat tggggagttg 1860
ctgtatctag agacgctagt tgcctatgga ttacgtgagc ttcctgaaag cacaagtcgg 1920
ctacaacgac tggcacgttt gtttgtttac tctggttgta agcttccagg tgggttggga 1980
aacttgataa acttgcaaga gcttgactgc gttgatgcct tgcatttgaa gcatgtggaa 2040
gagctcggca aactaacaaa tctgagaaag ctgagtatta aattggacac tggtgggatt 2100
gaaggcaaca aattagaaga gtccaaggaa aagctggtgt cctctctctg taaactggac 2160
gaatgcggcc tcctttccct gagtattgat tattatctga gagaaaagga tggagaagag 2220
ccgttcctcc ctgctttggg ttgtatccag gaggtttttg tatatggaca agatatcagc 2280
cggattagca gatggctcgc gtcacttccc aacctacacg ggttgctttt ggacaatccg 2340
aagatagagc agcaggatat tgagatgatt ggattgatac ccaatctgat cgatttgaca 2400
ctgtatttac gcataacaga cgacgctggg cggctcatca tcaagcgtga aggatttcaa 2460
cagttgcaga ggtttgagct ttcccgtatt cgtatggggg atttaatgtt tgaaccgggg 2520
gctatgccga ggctcaagga gcttatactc taccacttca tcgaaaagcc caaatctggt 2580
gcggtcgatt tcgactttgg catccaacac ctctccagcc ttgctcgcct cactgtcggc 2640
ttattatgcg ttggctcgac ggcggcggaa gtagaggctg cggaggatgc tttcaagagc 2700
atggccgagg cgaaccccaa ccgtccaata ctggagatga caagacttta tccgcagcat 2760
atgttacaag acgagcaaat aggtatgaca ggctcagcta caacgccggc cgtcaagtcc 2820
<210> 13
<211> 5915
<212> DNA
<213> 栽培一粒小麦
<400> 13
atggcggaag ttctggtgag cgcatccaca ggcgcaatgg gttccctcct ccggaagttg 60
ggagctatgt tgacggatga atacaagctg ctcaagaacg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgcg ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacacgagt cctcctgctc cgaggcggct 300
catggcgtcg ccaagctcat ggacaagtgc aagaacttgc tgccggacat caagacccgc 360
cgcaggatcg ccaaggaggt caaagacatc aagaaagaaa ttaaggatgt cagcgacaga 420
ttttcaaggt accagatctt gttctcttta cagtactatt agtacctacc atcagatttg 480
tgaccgattt gttgccactc aaggtacaag attgacgagt cctcctcctc tatgccggca 540
aaaggcaagg tcgaccctcg acttcgtgca gtctacaaag acgccgcaga gctcgtcgga 600
attgatggac caaaggatga acttgtcaag tggctgaatg agaaagaggg ccaatcactg 660
aaatctgtct ctattgttgg atatggaggg ttgggcaaga ccacgctcgc caaccagatc 720
cgggtcaacc ttggagcgac cttcgactgc ggggcttttg tctcgatttc acgcaagcct 780
gacatgaagg cgatattaag atctatatta tcacaaatca ccaagaaaga cgatgcttgc 840
tctagattag atgatataca actcatcatc gacaagattc gagaattcct ccaagacaca 900
aggtatgtat gtgagatctg ctacgaagta acattgctta attagctcat gcataatcgg 960
ttgcctagaa ttttgctaat gaagattgct ttgtgtgatt tactttcgtc ctcaacttgt 1020
atgactgcgc ttaaactttc aagtcattgt ttcttgcaac ttatgtcgat ctgttcacta 1080
attaagatat agctaaaatg tgtttgccga tttctgtcgc cacacatttt ctaatttgat 1140
tgccacaagt ttgttaaact tgcggctatg ttctgtttat taaataattg tgacatggcg 1200
tcagtcaaaa caataaagta catgcaattc cctctctttt gtacaagata aatatccgca 1260
cgcactacat gactgcaaat atatctatct tctaaggacc caaagcactt gcatgtgcag 1320
accattgtta ccggatatgc agtacattag gtttgtgatt agccacttta agaacatagg 1380
gtttctagag gctataaacc tacggttatc agtattataa tcttgaatgg atatcaaaca 1440
tccgttgaag aatctatgcc cataacacaa aaagagaaaa gggaagaatt aatcatagtc 1500
agatcggacc agcctgacta gcgtaacata atgacaacag taggttgttg cgtctttcct 1560
ggtttcgtag caatttgcat gacgaattga ggcgcagagc caccactcaa tatgtggtcc 1620
taccaatcat gaggaaataa cttgttacca aaatgacaaa agaaaatagg tagtccccat 1680
cattgtttca atatctatcc tcttgcgaac atgctattgg cagtggcaaa acattgtacc 1740
ttgccactgg caacagctgc ctttactcgg cctgagcatt catatttttt tcctcttggt 1800
tgttaaatct tgctattttt cgcttgcctt ctatggcaat cttttttttt cttatattgc 1860
caccaaattg tatctaagag caactccaac acgccgacgc aatttttgtc catttagatc 1920
ggccaagcgg acggggtgtc tggttttgcc tttgggtggt gcgcctacgc attagtccgc 1980
gtgggcattt aaaaaactta aaaagcttaa aataattaaa tataaataaa aatgcatatt 2040
aaagcccatc aaagtccaca taattaaaca taatacatta atatattaat aaaaaacagc 2100
cgccccagcc cgcagcccta ggcgtcttcg tcggaggagt gggtgaggtc gaagaatgcc 2160
ggcagctcag cccacgggaa ggcctgctgg tgggcagcca gcaccgcctg ctccgctgtc 2220
tgcggcggcg gcggccctac ctggcaccgc gcacgcctcc tcctcgcgct cctgccgctc 2280
ctcctcctca tcgcacgccc aacgaagttt tggcgctcct ccccctcggg ttcccgctgc 2340
atgcggacct ggccgtcgta ccgctcctcc tccagcgtgc ggtccttcca cgccacgagg 2400
taggccgcct cctcctccat cacgcacagc caaatgggcg gcttgctcat ccgcttgcag 2460
acgatgccct gccattggta aacgccggtg ggcgcgggtg gtgctagcgc tggtgcaggc 2520
ggtgacgatg tggctggtgg tcccggcagg tagacactat agttggcggc cgatagctgc 2580
agcgtgtcat cgaggccaga ccactaggag tcctcgcact aagactcctc ggtgacgtga 2640
tgggccccct cctccaatgc cttgaggaac gccgcctcct cctcaaccta ctatttgacc 2700
accggtggcg gcggtggagg gcaggcttcg agctggacgg cgtcgcggag gcgcacatgg 2760
tgctcgatgg tgaaccagac ttctcagtta ggggagtctg gcctcgatgg gtggagtcgc 2820
gacgctgctc tggcgtcagg agagcacgcc gggcccgaat ctcgttggcg cgcgcacgct 2880
cggactgtgg caccgccggc actggaatgt ggtgggggtc gaggtgccag ccgtggggga 2940
agttcacgtc cggccatggc agtgagacgc ggtactcccg gtggtactac gcgtggtgca 3000
ccgggatgta ctggtggtcc cgtggcgctg ccgccctccc atcccgcagc gccgacacca 3060
atgcgaaccc cttcgctgcc ggcctcaccg gcccttgcaa cgagccggcc tcgtggtcgt 3120
gcttgccttg cccttacctt tgccaaagaa acccatgacg gctagggttt tctgtcaccg 3180
gcgagggagc agatgtggac tagagtggga ggagaagtgg atgaggcccg ccccgccgca 3240
cccttgcaat taaaaagggc gtgatccgga cgtttctagt cactgccatg cgggcccgag 3300
gtaggtgggc ggcattgact acgtctgtgg tggtagttgg ctggcagaca cgccagcccg 3360
aggcgcacac cgagcggacg cgcaacacgt ccgtctcgtg ttcgtgtgaa cgcaaatcag 3420
gcacaaattt gggctggaaa tgcgtctaca tggatgccga acggacgtga tttgggtttg 3480
tgtccgcacg ttgggccggc gtttttgtgc ctttcgaccc taatggacac acacggacca 3540
aatccgttgg cgtgttagag ttgcttgtac ctagtactac tatgtgtgga gttgttatct 3600
ttgagtttct cccccatctg tagctgtgta ttattgtgag atgctctcag gatttcactt 3660
attttagata atgacaactt tcttgatgct agacaattac atcaaggtgt attatatcta 3720
ggatgcacac agatgtgaga ttgtttcagg attttaggtt acttggtagg tgttactata 3780
aatatctaat gagctctaat gtgtatgttc atgaaactgt gttgcaggta ctttatcata 3840
attgatgata tatgggagtt aggaacatgg gaaactttga aatgtgcatt tgtcaagaat 3900
acattgggca gcagaataat tatcacaaca cgtatagttg atgttgccaa atcttgttct 3960
ccttctagtg aagatcttgt ctatgagatg aaaccactca gtgaggctga ctcgaagaaa 4020
ttgtttttca agaggatatt tggctgtgaa gaaagctgtc ctgatagctt gaaagaagct 4080
gccaatgata ttttgaaaaa atgccgtggc ctacctctgg ccatcaatgc catttctagc 4140
ttgttggtca caacaaggga aacaaaagaa gagtgggatc gggtgcgaca ttcaatacgc 4200
tcttcaaagg tcaaaagtga tataatagag accatgaatt acatattgtc tcttagttat 4260
tttgatcttc cccaccacct aagaagctgc ttattgtacc tggctctgtt tcctgaagac 4320
caattgattg gaaggaagcg gttagtacgc agatggattt ctgaaggctt tatccatgga 4380
gaaagtggac aagatctcat ggaattagga gaggaatatt ttcaccagct tgtgaacaga 4440
agtctaatac agcccggcaa catagggtat gacggcaagg cggagtattg ccgagtccat 4500
gacaccatcc ttgatttcct aattgacaaa tcctctgaag agaacatgtg tactgtcctg 4560
aagaaacaat gcaagcctaa tggcatagtt cgtcggctct ctctaatggg gaatgaggat 4620
gaagaaatca tggagcaatt ggatctgtca cacgctcggt caatcagtgc ttttggggat 4680
atcaagctat tgccttcgct tgggaggtca aaatgcttgc gcgtgctaga cttgcaaggc 4740
tgctatcagt tgaaaaatca tcatatcaag gatattgaaa gactctatca gctgagatac 4800
ttggatatct cttttacagg aatcacggag cttcccaggc aaattgggga gttgctgtat 4860
ctagagacgc tagttagtac atcctatgga ttacgtgagc ttcctgaaag cacaagtcgg 4920
ctacaacgac tggcacgttt gtttgttgac agcggttgta agcttccaga tgggttggga 4980
aacttgataa acttgcaaga gcttgactgc gttgatgcct tgcagttgaa gcatgtggaa 5040
gagctcggca aactaacaaa tctgagaaag ctgaggatta aattggacac tgatgggatt 5100
gaaggcaaca aattagaaca gtccaaggaa aagctggtgt cctctctctg taaactggac 5160
gaatgcggcc tcctttccct gagtatttat tattatctga gagaaaagga tggagaagag 5220
ccgttcctcc ctgctttggg ttgtatccag gaggtttttg tacatggaca agatatcagc 5280
cggattagca gatggctcgc gtcacttccc aacctacaca ggttgttttt gaaccaaccg 5340
aagatagagc agcaggatat tgagatgatt ggattgatac ccaatctgat ggatttgaca 5400
ctgtatttat gcataacaga cgacgctggg cggctcatca tcaagcgtga aggatttcaa 5460
cagttgcaga ggtttgagct ttcccgtatt cgtatggggg atttaatgtt tgaaccgggg 5520
gctatgccga ggctcaagga gcttatactc tacgacttca tcggaaagcc caaatctggt 5580
gcggtcgatt tcgactttgg catccaacac ctctccagcc ttgctcgcct cactgtcggc 5640
ttatcctgcg ttggctcgac ggtggcggaa gtagaggccg cggaggatgc tttcaagagc 5700
atggccgagg cgaaccccaa ccgtccaata ctggagatga caagatataa tacgcagcat 5760
atgttacaag acgagcaaat aggtatgaca ggctcaggta ctattaacta gtttgtactt 5820
ttgtcttggt gagcggtaga tcaaaagatg tgcatatgct tcaatctttt ttccggtaat 5880
ctatttcagc tacaacgccg gccgtcaagt cctag 5915
<210> 14
<211> 940
<212> PRT
<213> 栽培一粒小麦
<400> 14
Met Ala Glu Val Leu Val Ser Ala Ser Thr Gly Ala Met Gly Ser Leu
1 5 10 15
Leu Arg Lys Leu Gly Ala Met Leu Thr Asp Glu Tyr Lys Leu Leu Lys
20 25 30
Asn Val Arg Gly Asp Ile Lys Phe Leu Lys Asp Glu Leu Glu Val Met
35 40 45
Cys Ala Phe Leu Leu Lys Met Ser Asp Val Glu Glu Pro Asp Glu Pro
50 55 60
Thr Lys Leu Arg Val Thr Ala Val Arg Glu Met Ser Tyr Lys Ile Glu
65 70 75 80
Asp Asn Ile Asp Lys Phe Met Val Leu Val Glu His Glu Ser Ser Cys
85 90 95
Ser Glu Ala Ala His Gly Val Ala Lys Leu Met Asp Lys Cys Lys Asn
100 105 110
Leu Leu Pro Asp Ile Lys Thr Arg Arg Arg Ile Ala Lys Glu Val Lys
115 120 125
Asp Ile Lys Lys Glu Ile Lys Asp Val Ser Asp Arg Phe Ser Arg Tyr
130 135 140
Lys Ile Asp Glu Ser Ser Ser Ser Met Pro Ala Lys Gly Lys Val Asp
145 150 155 160
Pro Arg Leu Arg Ala Val Tyr Lys Asp Ala Ala Glu Leu Val Gly Ile
165 170 175
Asp Gly Pro Lys Asp Glu Leu Val Lys Trp Leu Asn Glu Lys Glu Gly
180 185 190
Gln Ser Leu Lys Ser Val Ser Ile Val Gly Tyr Gly Gly Leu Gly Lys
195 200 205
Thr Thr Leu Ala Asn Gln Ile Arg Val Asn Leu Gly Ala Thr Phe Asp
210 215 220
Cys Gly Ala Phe Val Ser Ile Ser Arg Lys Pro Asp Met Lys Ala Ile
225 230 235 240
Leu Arg Ser Ile Leu Ser Gln Ile Thr Lys Lys Asp Asp Ala Cys Ser
245 250 255
Arg Leu Asp Asp Ile Gln Leu Ile Ile Asp Lys Ile Arg Glu Phe Leu
260 265 270
Gln Asp Thr Arg Tyr Phe Ile Ile Ile Asp Asp Ile Trp Glu Leu Gly
275 280 285
Thr Trp Glu Thr Leu Lys Cys Ala Phe Val Lys Asn Thr Leu Gly Ser
290 295 300
Arg Ile Ile Ile Thr Thr Arg Ile Val Asp Val Ala Lys Ser Cys Ser
305 310 315 320
Pro Ser Ser Glu Asp Leu Val Tyr Glu Met Lys Pro Leu Ser Glu Ala
325 330 335
Asp Ser Lys Lys Leu Phe Phe Lys Arg Ile Phe Gly Cys Glu Glu Ser
340 345 350
Cys Pro Asp Ser Leu Lys Glu Ala Ala Asn Asp Ile Leu Lys Lys Cys
355 360 365
Arg Gly Leu Pro Leu Ala Ile Asn Ala Ile Ser Ser Leu Leu Val Thr
370 375 380
Thr Arg Glu Thr Lys Glu Glu Trp Asp Arg Val Arg His Ser Ile Arg
385 390 395 400
Ser Ser Lys Val Lys Ser Asp Ile Ile Glu Thr Met Asn Tyr Ile Leu
405 410 415
Ser Leu Ser Tyr Phe Asp Leu Pro His His Leu Arg Ser Cys Leu Leu
420 425 430
Tyr Leu Ala Leu Phe Pro Glu Asp Gln Leu Ile Gly Arg Lys Arg Leu
435 440 445
Val Arg Arg Trp Ile Ser Glu Gly Phe Ile His Gly Glu Ser Gly Gln
450 455 460
Asp Leu Met Glu Leu Gly Glu Glu Tyr Phe His Gln Leu Val Asn Arg
465 470 475 480
Ser Leu Ile Gln Pro Gly Asn Ile Gly Tyr Asp Gly Lys Ala Glu Tyr
485 490 495
Cys Arg Val His Asp Thr Ile Leu Asp Phe Leu Ile Asp Lys Ser Ser
500 505 510
Glu Glu Asn Met Cys Thr Val Leu Lys Lys Gln Cys Lys Pro Asn Gly
515 520 525
Ile Val Arg Arg Leu Ser Leu Met Gly Asn Glu Asp Glu Glu Ile Met
530 535 540
Glu Gln Leu Asp Leu Ser His Ala Arg Ser Ile Ser Ala Phe Gly Asp
545 550 555 560
Ile Lys Leu Leu Pro Ser Leu Gly Arg Ser Lys Cys Leu Arg Val Leu
565 570 575
Asp Leu Gln Gly Cys Tyr Gln Leu Lys Asn His His Ile Lys Asp Ile
580 585 590
Glu Arg Leu Tyr Gln Leu Arg Tyr Leu Asp Ile Ser Phe Thr Gly Ile
595 600 605
Thr Glu Leu Pro Arg Gln Ile Gly Glu Leu Leu Tyr Leu Glu Thr Leu
610 615 620
Val Ser Thr Ser Tyr Gly Leu Arg Glu Leu Pro Glu Ser Thr Ser Arg
625 630 635 640
Leu Gln Arg Leu Ala Arg Leu Phe Val Asp Ser Gly Cys Lys Leu Pro
645 650 655
Asp Gly Leu Gly Asn Leu Ile Asn Leu Gln Glu Leu Asp Cys Val Asp
660 665 670
Ala Leu Gln Leu Lys His Val Glu Glu Leu Gly Lys Leu Thr Asn Leu
675 680 685
Arg Lys Leu Arg Ile Lys Leu Asp Thr Asp Gly Ile Glu Gly Asn Lys
690 695 700
Leu Glu Gln Ser Lys Glu Lys Leu Val Ser Ser Leu Cys Lys Leu Asp
705 710 715 720
Glu Cys Gly Leu Leu Ser Leu Ser Ile Tyr Tyr Tyr Leu Arg Glu Lys
725 730 735
Asp Gly Glu Glu Pro Phe Leu Pro Ala Leu Gly Cys Ile Gln Glu Val
740 745 750
Phe Val His Gly Gln Asp Ile Ser Arg Ile Ser Arg Trp Leu Ala Ser
755 760 765
Leu Pro Asn Leu His Arg Leu Phe Leu Asn Gln Pro Lys Ile Glu Gln
770 775 780
Gln Asp Ile Glu Met Ile Gly Leu Ile Pro Asn Leu Met Asp Leu Thr
785 790 795 800
Leu Tyr Leu Cys Ile Thr Asp Asp Ala Gly Arg Leu Ile Ile Lys Arg
805 810 815
Glu Gly Phe Gln Gln Leu Gln Arg Phe Glu Leu Ser Arg Ile Arg Met
820 825 830
Gly Asp Leu Met Phe Glu Pro Gly Ala Met Pro Arg Leu Lys Glu Leu
835 840 845
Ile Leu Tyr Asp Phe Ile Gly Lys Pro Lys Ser Gly Ala Val Asp Phe
850 855 860
Asp Phe Gly Ile Gln His Leu Ser Ser Leu Ala Arg Leu Thr Val Gly
865 870 875 880
Leu Ser Cys Val Gly Ser Thr Val Ala Glu Val Glu Ala Ala Glu Asp
885 890 895
Ala Phe Lys Ser Met Ala Glu Ala Asn Pro Asn Arg Pro Ile Leu Glu
900 905 910
Met Thr Arg Tyr Asn Thr Gln His Met Leu Gln Asp Glu Gln Ile Gly
915 920 925
Met Thr Gly Ser Ala Thr Thr Pro Ala Val Lys Ser
930 935 940
<210> 15
<211> 2820
<212> DNA
<213> 栽培一粒小麦
<400> 15
atggcggaag ttctggtgag cgcatccaca ggcgcaatgg gttccctcct ccggaagttg 60
ggagctatgt tgacggatga atacaagctg ctcaagaacg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgcg ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacacgagt cctcctgctc cgaggcggct 300
catggcgtcg ccaagctcat ggacaagtgc aagaacttgc tgccggacat caagacccgc 360
cgcaggatcg ccaaggaggt caaagacatc aagaaagaaa ttaaggatgt cagcgacaga 420
ttttcaaggt acaagattga cgagtcctcc tcctctatgc cggcaaaagg caaggtcgac 480
cctcgacttc gtgcagtcta caaagacgcc gcagagctcg tcggaattga tggaccaaag 540
gatgaacttg tcaagtggct gaatgagaaa gagggccaat cactgaaatc tgtctctatt 600
gttggatatg gagggttggg caagaccacg ctcgccaacc agatccgggt caaccttgga 660
gcgaccttcg actgcggggc ttttgtctcg atttcacgca agcctgacat gaaggcgata 720
ttaagatcta tattatcaca aatcaccaag aaagacgatg cttgctctag attagatgat 780
atacaactca tcatcgacaa gattcgagaa ttcctccaag acacaaggta ctttatcata 840
attgatgata tatgggagtt aggaacatgg gaaactttga aatgtgcatt tgtcaagaat 900
acattgggca gcagaataat tatcacaaca cgtatagttg atgttgccaa atcttgttct 960
ccttctagtg aagatcttgt ctatgagatg aaaccactca gtgaggctga ctcgaagaaa 1020
ttgtttttca agaggatatt tggctgtgaa gaaagctgtc ctgatagctt gaaagaagct 1080
gccaatgata ttttgaaaaa atgccgtggc ctacctctgg ccatcaatgc catttctagc 1140
ttgttggtca caacaaggga aacaaaagaa gagtgggatc gggtgcgaca ttcaatacgc 1200
tcttcaaagg tcaaaagtga tataatagag accatgaatt acatattgtc tcttagttat 1260
tttgatcttc cccaccacct aagaagctgc ttattgtacc tggctctgtt tcctgaagac 1320
caattgattg gaaggaagcg gttagtacgc agatggattt ctgaaggctt tatccatgga 1380
gaaagtggac aagatctcat ggaattagga gaggaatatt ttcaccagct tgtgaacaga 1440
agtctaatac agcccggcaa catagggtat gacggcaagg cggagtattg ccgagtccat 1500
gacaccatcc ttgatttcct aattgacaaa tcctctgaag agaacatgtg tactgtcctg 1560
aagaaacaat gcaagcctaa tggcatagtt cgtcggctct ctctaatggg gaatgaggat 1620
gaagaaatca tggagcaatt ggatctgtca cacgctcggt caatcagtgc ttttggggat 1680
atcaagctat tgccttcgct tgggaggtca aaatgcttgc gcgtgctaga cttgcaaggc 1740
tgctatcagt tgaaaaatca tcatatcaag gatattgaaa gactctatca gctgagatac 1800
ttggatatct cttttacagg aatcacggag cttcccaggc aaattgggga gttgctgtat 1860
ctagagacgc tagttagtac atcctatgga ttacgtgagc ttcctgaaag cacaagtcgg 1920
ctacaacgac tggcacgttt gtttgttgac agcggttgta agcttccaga tgggttggga 1980
aacttgataa acttgcaaga gcttgactgc gttgatgcct tgcagttgaa gcatgtggaa 2040
gagctcggca aactaacaaa tctgagaaag ctgaggatta aattggacac tgatgggatt 2100
gaaggcaaca aattagaaca gtccaaggaa aagctggtgt cctctctctg taaactggac 2160
gaatgcggcc tcctttccct gagtatttat tattatctga gagaaaagga tggagaagag 2220
ccgttcctcc ctgctttggg ttgtatccag gaggtttttg tacatggaca agatatcagc 2280
cggattagca gatggctcgc gtcacttccc aacctacaca ggttgttttt gaaccaaccg 2340
aagatagagc agcaggatat tgagatgatt ggattgatac ccaatctgat ggatttgaca 2400
ctgtatttat gcataacaga cgacgctggg cggctcatca tcaagcgtga aggatttcaa 2460
cagttgcaga ggtttgagct ttcccgtatt cgtatggggg atttaatgtt tgaaccgggg 2520
gctatgccga ggctcaagga gcttatactc tacgacttca tcggaaagcc caaatctggt 2580
gcggtcgatt tcgactttgg catccaacac ctctccagcc ttgctcgcct cactgtcggc 2640
ttatcctgcg ttggctcgac ggtggcggaa gtagaggccg cggaggatgc tttcaagagc 2700
atggccgagg cgaaccccaa ccgtccaata ctggagatga caagatataa tacgcagcat 2760
atgttacaag acgagcaaat aggtatgaca ggctcagcta caacgccggc cgtcaagtcc 2820
<210> 16
<211> 5914
<212> DNA
<213> 栽培一粒小麦
<400> 16
atggcggaag ttctggtgag cgcatccaca ggggcaatgg gttccctcct gcggaagttg 60
ggagctatgt tgacggatga atacaagctg ctcaagaacg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgcg ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacaggaac acgggtcctc ctgttccgag 300
gcggctcatg gcgtcgccaa gctcatggac aagtgcaaga acttgctgcc ggacatcaag 360
gcccgccgca ggatcgccaa ggaggtcaaa gacatcaaga aagaaattaa ggatgtcagc 420
gacagatttt caaggtacca gatcttgttc tccttacagt actattagta cctaccatca 480
gatttgtgac cgatttgttg ccactcaagg tacaagattg acgactcctc ctcctctatg 540
ccggcaaaag acaaggtcga ccctcgactt cgtgcagtct acaaagacgc cgcagagctc 600
gtcggaattg atggaccaaa ggatgaactt gtcaagtggc tgaatgagaa agaaggccaa 660
tcactgaaat ctgtctctat tgttggatat ggagggttgg gcaagaccac gctcgccaac 720
cagatccggg tcaaccttgg agcgaccttc gactgcgggg cttttgtctc gatttcacgc 780
aagcctgaca tgaaggcgat attaagatct atattatcac aaatcaccaa gaaagacgat 840
gcttgctcta gattagatga tatacaactc atcatcgaca agattcgaga attcctccaa 900
gacacaaggt atgtatgtga gatctgctac gaagtaacat tgcttaatta gctcatgcat 960
aatcggttgc ctagaatttt gctaatgaag attgctttgt gtgatttact ttcgtcctca 1020
acttgtatga ctgcgcttaa actttcaagt cattgtttct tgcaacttat gtcgatctgt 1080
tcactaatta agatatagct aaaatgtgtt tgccgatttc tgtcgccaca cattttctaa 1140
tttgattgcc acaagtttgt taaacttgcg gctatgttct gtttattaaa taattgtgac 1200
atggcgtcag tcaaaacaat aaagtacatg caattccctc tcttttgtac aagataaata 1260
tccgcacgca ctacatgact gcaaatatat ctatcttcta aggacccaaa gcacttgcat 1320
gtgcagacca ttgttaccgg atatgcagta cattaggttt gtgattagcc actttaagaa 1380
catagggttt ctagaggcta taaacctacg gttatcagta ttataatctt gaatggatat 1440
caaacatccg ttgaagaatc tatgcccata acacaaaaag agaaaaggga agaattaatc 1500
atagtcagat cggaccagcc tgactagcgt aacataatga caacagtagg ttgttgcgtc 1560
tttcctggtt tcgtagcaat ttgcatgacg aattgaggcg cagagccacc actcaatatg 1620
tggtcctacc aatcatgagg aaataacttg ttaccaaaat gacaaaagaa aataggtagt 1680
ccccatcatt gtttcaatat ctatcctctt gcgaacatgc tattggcagt ggcaaaacat 1740
tgtaccttgc cactggcaac agctgccttt actcggcctg agcattcata tttttttcct 1800
cttggttgtt aaatcttgct atttttcgct tgccttctat ggcaatcttt tttttttctt 1860
atattgccac caaattgtat ctaagagcaa ctccaacacg ccgacgcaat ttttgtccat 1920
ttagatcggc caagcggacg gggtgtctgg ttttgccttt gggtggtgcg cctacgcatt 1980
agtccgcgtg ggcatttaaa aaacttaaaa agcttaaaat aattaaatat aaataaaaat 2040
gcatattaaa gcccgtcaaa gtccacataa ttaaacataa tacattaata tattaataaa 2100
aaacagccgc cccagcccgc agccctaggc gtcttcgtcg gaggagtggg tgaggtcgaa 2160
gaatgccggc agctcagccc acgggaaggc ctgctggtgg gcagccagca ccgcctgctc 2220
cgctgtctgc ggcggcggcc ctacctggca ccgcgcacgc ctcctcctcg cgctcctgcc 2280
gctcctcctc ctcatcgcac gcccaacgaa gttttggcgc tcctccccct cgggttcccg 2340
ctgcatgcgg acctggccgt cgtaccgctc ctcctccagc gtgcggtcct tccacgccac 2400
gaggtaggcc gcctcctcct ccatcacgca cagccaaatg ggcggcttgc tcatccgctt 2460
gcagacgatg ccctgccatt ggtaaacgcc ggtgggcgcg ggtggtgcta gcgctggtgc 2520
aggcggtgac gatgtggctg gtggtcccgg caggtagaca ctatagctgg cggccgatag 2580
ctgcagcgtg tcatcgaggc cagaccacta ggagtcctcg cactaagact cctcggtgac 2640
gtgatgggcc ccctcctcca atgccttgag gaacgccgcc tcctcctcaa cctactattt 2700
gaccaccggt ggcgggcggt ggagggcagg cttcgagctg gacggcgtcg cggaggcgca 2760
catggtgctc gatggtgaac cagacttctc agttagggga gtctggcctc gatgggtgga 2820
gtcgcgacgc tgctctggcg tcaggagagc acgccgggcc cgaatctcgt tggcgcgcgc 2880
acgctcggac tgtggcaccg ccggcactgg aatgtggtgg gggtcgaggt gccagccgtg 2940
ggggaagttc acgtccggcc acggcagtga gacgcggtac tcccggtggt actacgcgtg 3000
gtgcaccagg atgtactggt ggtcccgtgg cgctgccgcc ctcccatccc gcagcgccga 3060
caccaatgcg aaccccttcg ctgccggcct caccggccct tgcaacgagc cggcctcgtg 3120
gtcgtgcttg ccttgccctt acctttgcca aagaaaccca tgacggctag ggttttctgt 3180
caccggcgag ggagcagatg tggactagag tgggaggaga agtggatgag gcccgccccg 3240
ccgcaccctt gcaattaaaa agggcatgat ccggacgttt ctagtcactg ccatgcgggc 3300
ccgaggtagg tgggcggcat tgactacgtc tgtggtggta gttggctggc agacacgcca 3360
gcccgaggcg cacaccgagc agacgtgcaa cacgtccgtc tcatgttcgt gtgaacgcaa 3420
atcaggcaca aatttgggct ggaaatgcgt ctacatggat gccgaacgga cgtgatttgg 3480
gtttgtgtcc gcacgttggg ccggcgtttt tgtgcctttc gaccctaatg gacacacacg 3540
gaccaaatcc gttggcgtgt tagagttgct tgtacctagt actactatgt gtggagttgt 3600
tatctttgag tttctccccc atctgtagct gtgtattatt gtgagatgct ctcaggattt 3660
cacttatttt agataatgac aactttcttg atgctagaca attacatcaa ggtgtattat 3720
atctaggatg cacacagatg tgagattgtt tcaggatttt aggttacttg gtaggtgtta 3780
ctataaatat ctaatgagct ctaatgtgta tgttcatgaa actgtgttgc aggtacttta 3840
tcataattga tgatatatgg gagttaggaa catgggaaac tttgaaatgt gcatttgtca 3900
agaatacatt gggcagcaga ataattatca caacacgtat agttgatgtt gccaaatctt 3960
gttctccttc tagtgaagat cttgtctatg agatgaaacc actcagtgag gctgactcga 4020
agaaattgtt tttcaagagg atatttggct gtgaagaaag ctgtcctgat agcttgaaag 4080
aagctgccaa tgatattttg aaaaaatgcc gtggcctacc tctggccatc aatgccattt 4140
ctagcttgtt ggtcacaaca agggaaacaa aagaagagtg ggatcgggtg cgacattcaa 4200
tacgctcttc aaaggtcaaa agtgatataa tagagaccat gaattacata ttgtctctta 4260
gttattttga tcttccccac cacctaagaa gctgcttatt gtacctggct ctgtttcctg 4320
aagaccaatt gattggaagg aagcggttag tacgcagatg gatttctgaa ggctttatcc 4380
atggagaaag tggacaagat ctcatggaat taggagagga atattttcac cagcttgtga 4440
acagaagtct aatacagccc ggcaacatag ggtatgacgg caaggcaatg tattgccgag 4500
tccatgacac catccttgat ttcctaattg acaaatcctc tgaagagaac atgtgtactg 4560
tcctaaagaa acaatgcaag cctaatggca tagttcgtcg gctctctcta atggggaatg 4620
aggatgaaga aatcgtggag caattggatc tgtcacacgc tcggtcaatc agtgcttttg 4680
gggatatcaa gctattgcct tcgcttggga ggtcaaaatg cttgcgtgtg ctagacttgc 4740
aagactgcga tcagttggaa aatcatcata tcaaggatat tgaaagactc tatcagctga 4800
gatacttgga tatctcttat acaggaatca cggagcttcc gaggcaaatt ggggagttgc 4860
tgtatctaga gacgctagat gcctctggat tacgtgagct tcctgaaagc acaagtcggc 4920
tacaacgact ggcacgtttg tttgttgaca gcggttgtaa gcttccagat gggttgggaa 4980
acttgataaa cttgcaagag cttgactgcg ttgatgcctt gcagttgaag catgtggaag 5040
agctcggcaa actaacaaat ctgagaaagc tgaagattaa attggacact ggtgggattg 5100
aaggcaacaa attagaagag tccaaggaaa agctggtgtc ctctctctgt aaactggacg 5160
aatgcggcct ccgttccctg agtattcgtt attatctgag agaaaaggat ggagaagagc 5220
cgttcctccc tgctttgggt tgtatccagg aggtttgtgt atatggacaa gatatcagcc 5280
ggattagcag atggctcgcg tcacttccca acctacacac gttgcttttg gactatccga 5340
agatagagca gcaggatatt gagatgattg gattgatacc caatctgatc gatttgacac 5400
tgcctctata caaaacagac gacgctgggc ggctcatcat caggcgtgaa ggatttcaac 5460
agttgcagaa gtttgaggct tacaatactc gtatgggggt tttaatgttt gaaccggggg 5520
ttatgccgag gctcaaggag cttaaactcc ataacttcat cgaaaagccc aaatctgctg 5580
cggtcgattt cgactttggc atccaacacc tctccagcct tgctcgcctc actgtcgact 5640
tatcctgcgt tggctggacg gcggcggaag tagaggctgc ggaggatgct ttcaagagca 5700
tggccgaggc gaaccccaac cgtccaatac tggagatgac aagatataat acgcagcata 5760
tgttacaaga cgagcaaata ggtatgacag gctcaggtac tattaactag tttgtacttt 5820
tgtcttggtg agcagtagat caaaagatgt gcatatgctt caatcttttt tccggtaatc 5880
tatttcagct acaacgccgg ccgtcaagtc ctag 5914
<210> 17
<211> 940
<212> PRT
<213> 栽培一粒小麦
<400> 17
Met Ala Glu Val Leu Val Ser Ala Ser Thr Gly Ala Met Gly Ser Leu
1 5 10 15
Leu Arg Lys Leu Gly Ala Met Leu Thr Asp Glu Tyr Lys Leu Leu Lys
20 25 30
Asn Val Arg Gly Asp Ile Lys Phe Leu Lys Asp Glu Leu Glu Val Met
35 40 45
Cys Ala Phe Leu Leu Lys Met Ser Asp Val Glu Glu Pro Asp Glu Pro
50 55 60
Thr Lys Leu Arg Val Thr Ala Val Arg Glu Met Ser Tyr Lys Ile Glu
65 70 75 80
Asp Asn Ile Asp Lys Phe Met Val Leu Val Glu Gln Glu His Gly Ser
85 90 95
Ser Cys Ser Glu Ala Ala His Gly Val Ala Lys Leu Met Asp Lys Cys
100 105 110
Lys Asn Leu Leu Pro Asp Ile Lys Ala Arg Arg Arg Ile Ala Lys Glu
115 120 125
Val Lys Asp Ile Lys Lys Glu Ile Lys Asp Val Ser Asp Arg Phe Ser
130 135 140
Arg Tyr Lys Ile Asp Asp Ser Ser Ser Ser Met Pro Ala Lys Asp Lys
145 150 155 160
Val Asp Pro Arg Leu Arg Ala Val Tyr Lys Asp Ala Ala Glu Leu Val
165 170 175
Gly Ile Asp Gly Pro Lys Asp Glu Leu Val Lys Trp Leu Asn Glu Lys
180 185 190
Glu Gly Gln Ser Leu Lys Ser Val Ser Ile Val Gly Tyr Gly Gly Leu
195 200 205
Gly Lys Thr Thr Leu Ala Asn Gln Ile Arg Val Asn Leu Gly Ala Thr
210 215 220
Phe Asp Cys Gly Ala Phe Val Ser Ile Ser Arg Lys Pro Asp Met Lys
225 230 235 240
Ala Ile Leu Arg Ser Ile Leu Ser Gln Ile Thr Lys Lys Asp Asp Ala
245 250 255
Cys Ser Arg Leu Asp Asp Ile Gln Leu Ile Ile Asp Lys Ile Arg Glu
260 265 270
Phe Leu Gln Asp Thr Arg Tyr Phe Ile Ile Ile Asp Asp Ile Trp Glu
275 280 285
Leu Gly Thr Trp Glu Thr Leu Lys Cys Ala Phe Val Lys Asn Thr Leu
290 295 300
Gly Ser Arg Ile Ile Ile Thr Thr Arg Ile Val Asp Val Ala Lys Ser
305 310 315 320
Cys Ser Pro Ser Ser Glu Asp Leu Val Tyr Glu Met Lys Pro Leu Ser
325 330 335
Glu Ala Asp Ser Lys Lys Leu Phe Phe Lys Arg Ile Phe Gly Cys Glu
340 345 350
Glu Ser Cys Pro Asp Ser Leu Lys Glu Ala Ala Asn Asp Ile Leu Lys
355 360 365
Lys Cys Arg Gly Leu Pro Leu Ala Ile Asn Ala Ile Ser Ser Leu Leu
370 375 380
Val Thr Thr Arg Glu Thr Lys Glu Glu Trp Asp Arg Val Arg His Ser
385 390 395 400
Ile Arg Ser Ser Lys Val Lys Ser Asp Ile Ile Glu Thr Met Asn Tyr
405 410 415
Ile Leu Ser Leu Ser Tyr Phe Asp Leu Pro His His Leu Arg Ser Cys
420 425 430
Leu Leu Tyr Leu Ala Leu Phe Pro Glu Asp Gln Leu Ile Gly Arg Lys
435 440 445
Arg Leu Val Arg Arg Trp Ile Ser Glu Gly Phe Ile His Gly Glu Ser
450 455 460
Gly Gln Asp Leu Met Glu Leu Gly Glu Glu Tyr Phe His Gln Leu Val
465 470 475 480
Asn Arg Ser Leu Ile Gln Pro Gly Asn Ile Gly Tyr Asp Gly Lys Ala
485 490 495
Met Tyr Cys Arg Val His Asp Thr Ile Leu Asp Phe Leu Ile Asp Lys
500 505 510
Ser Ser Glu Glu Asn Met Cys Thr Val Leu Lys Lys Gln Cys Lys Pro
515 520 525
Asn Gly Ile Val Arg Arg Leu Ser Leu Met Gly Asn Glu Asp Glu Glu
530 535 540
Ile Val Glu Gln Leu Asp Leu Ser His Ala Arg Ser Ile Ser Ala Phe
545 550 555 560
Gly Asp Ile Lys Leu Leu Pro Ser Leu Gly Arg Ser Lys Cys Leu Arg
565 570 575
Val Leu Asp Leu Gln Asp Cys Asp Gln Leu Glu Asn His His Ile Lys
580 585 590
Asp Ile Glu Arg Leu Tyr Gln Leu Arg Tyr Leu Asp Ile Ser Tyr Thr
595 600 605
Gly Ile Thr Glu Leu Pro Arg Gln Ile Gly Glu Leu Leu Tyr Leu Glu
610 615 620
Thr Leu Asp Ala Ser Gly Leu Arg Glu Leu Pro Glu Ser Thr Ser Arg
625 630 635 640
Leu Gln Arg Leu Ala Arg Leu Phe Val Asp Ser Gly Cys Lys Leu Pro
645 650 655
Asp Gly Leu Gly Asn Leu Ile Asn Leu Gln Glu Leu Asp Cys Val Asp
660 665 670
Ala Leu Gln Leu Lys His Val Glu Glu Leu Gly Lys Leu Thr Asn Leu
675 680 685
Arg Lys Leu Lys Ile Lys Leu Asp Thr Gly Gly Ile Glu Gly Asn Lys
690 695 700
Leu Glu Glu Ser Lys Glu Lys Leu Val Ser Ser Leu Cys Lys Leu Asp
705 710 715 720
Glu Cys Gly Leu Arg Ser Leu Ser Ile Arg Tyr Tyr Leu Arg Glu Lys
725 730 735
Asp Gly Glu Glu Pro Phe Leu Pro Ala Leu Gly Cys Ile Gln Glu Val
740 745 750
Cys Val Tyr Gly Gln Asp Ile Ser Arg Ile Ser Arg Trp Leu Ala Ser
755 760 765
Leu Pro Asn Leu His Thr Leu Leu Leu Asp Tyr Pro Lys Ile Glu Gln
770 775 780
Gln Asp Ile Glu Met Ile Gly Leu Ile Pro Asn Leu Ile Asp Leu Thr
785 790 795 800
Leu Pro Leu Tyr Lys Thr Asp Asp Ala Gly Arg Leu Ile Ile Arg Arg
805 810 815
Glu Gly Phe Gln Gln Leu Gln Lys Phe Glu Ala Tyr Asn Thr Arg Met
820 825 830
Gly Val Leu Met Phe Glu Pro Gly Val Met Pro Arg Leu Lys Glu Leu
835 840 845
Lys Leu His Asn Phe Ile Glu Lys Pro Lys Ser Ala Ala Val Asp Phe
850 855 860
Asp Phe Gly Ile Gln His Leu Ser Ser Leu Ala Arg Leu Thr Val Asp
865 870 875 880
Leu Ser Cys Val Gly Trp Thr Ala Ala Glu Val Glu Ala Ala Glu Asp
885 890 895
Ala Phe Lys Ser Met Ala Glu Ala Asn Pro Asn Arg Pro Ile Leu Glu
900 905 910
Met Thr Arg Tyr Asn Thr Gln His Met Leu Gln Asp Glu Gln Ile Gly
915 920 925
Met Thr Gly Ser Ala Thr Thr Pro Ala Val Lys Ser
930 935 940
<210> 18
<211> 2820
<212> DNA
<213> 栽培一粒小麦
<400> 18
atggcggaag ttctggtgag cgcatccaca ggggcaatgg gttccctcct gcggaagttg 60
ggagctatgt tgacggatga atacaagctg ctcaagaacg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgcg ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacaggaac acgggtcctc ctgttccgag 300
gcggctcatg gcgtcgccaa gctcatggac aagtgcaaga acttgctgcc ggacatcaag 360
gcccgccgca ggatcgccaa ggaggtcaaa gacatcaaga aagaaattaa ggatgtcagc 420
gacagatttt caaggtacaa gattgacgac tcctcctcct ctatgccggc aaaagacaag 480
gtcgaccctc gacttcgtgc agtctacaaa gacgccgcag agctcgtcgg aattgatgga 540
ccaaaggatg aacttgtcaa gtggctgaat gagaaagaag gccaatcact gaaatctgtc 600
tctattgttg gatatggagg gttgggcaag accacgctcg ccaaccagat ccgggtcaac 660
cttggagcga ccttcgactg cggggctttt gtctcgattt cacgcaagcc tgacatgaag 720
gcgatattaa gatctatatt atcacaaatc accaagaaag acgatgcttg ctctagatta 780
gatgatatac aactcatcat cgacaagatt cgagaattcc tccaagacac aaggtacttt 840
atcataattg atgatatatg ggagttagga acatgggaaa ctttgaaatg tgcatttgtc 900
aagaatacat tgggcagcag aataattatc acaacacgta tagttgatgt tgccaaatct 960
tgttctcctt ctagtgaaga tcttgtctat gagatgaaac cactcagtga ggctgactcg 1020
aagaaattgt ttttcaagag gatatttggc tgtgaagaaa gctgtcctga tagcttgaaa 1080
gaagctgcca atgatatttt gaaaaaatgc cgtggcctac ctctggccat caatgccatt 1140
tctagcttgt tggtcacaac aagggaaaca aaagaagagt gggatcgggt gcgacattca 1200
atacgctctt caaaggtcaa aagtgatata atagagacca tgaattacat attgtctctt 1260
agttattttg atcttcccca ccacctaaga agctgcttat tgtacctggc tctgtttcct 1320
gaagaccaat tgattggaag gaagcggtta gtacgcagat ggatttctga aggctttatc 1380
catggagaaa gtggacaaga tctcatggaa ttaggagagg aatattttca ccagcttgtg 1440
aacagaagtc taatacagcc cggcaacata gggtatgacg gcaaggcaat gtattgccga 1500
gtccatgaca ccatccttga tttcctaatt gacaaatcct ctgaagagaa catgtgtact 1560
gtcctaaaga aacaatgcaa gcctaatggc atagttcgtc ggctctctct aatggggaat 1620
gaggatgaag aaatcgtgga gcaattggat ctgtcacacg ctcggtcaat cagtgctttt 1680
ggggatatca agctattgcc ttcgcttggg aggtcaaaat gcttgcgtgt gctagacttg 1740
caagactgcg atcagttgga aaatcatcat atcaaggata ttgaaagact ctatcagctg 1800
agatacttgg atatctctta tacaggaatc acggagcttc cgaggcaaat tggggagttg 1860
ctgtatctag agacgctaga tgcctctgga ttacgtgagc ttcctgaaag cacaagtcgg 1920
ctacaacgac tggcacgttt gtttgttgac agcggttgta agcttccaga tgggttggga 1980
aacttgataa acttgcaaga gcttgactgc gttgatgcct tgcagttgaa gcatgtggaa 2040
gagctcggca aactaacaaa tctgagaaag ctgaagatta aattggacac tggtgggatt 2100
gaaggcaaca aattagaaga gtccaaggaa aagctggtgt cctctctctg taaactggac 2160
gaatgcggcc tccgttccct gagtattcgt tattatctga gagaaaagga tggagaagag 2220
ccgttcctcc ctgctttggg ttgtatccag gaggtttgtg tatatggaca agatatcagc 2280
cggattagca gatggctcgc gtcacttccc aacctacaca cgttgctttt ggactatccg 2340
aagatagagc agcaggatat tgagatgatt ggattgatac ccaatctgat cgatttgaca 2400
ctgcctctat acaaaacaga cgacgctggg cggctcatca tcaggcgtga aggatttcaa 2460
cagttgcaga agtttgaggc ttacaatact cgtatggggg ttttaatgtt tgaaccgggg 2520
gttatgccga ggctcaagga gcttaaactc cataacttca tcgaaaagcc caaatctgct 2580
gcggtcgatt tcgactttgg catccaacac ctctccagcc ttgctcgcct cactgtcgac 2640
ttatcctgcg ttggctggac ggcggcggaa gtagaggctg cggaggatgc tttcaagagc 2700
atggccgagg cgaaccccaa ccgtccaata ctggagatga caagatataa tacgcagcat 2760
atgttacaag acgagcaaat aggtatgaca ggctcagcta caacgccggc cgtcaagtcc 2820
<210> 19
<211> 5912
<212> DNA
<213> 野生一粒小麦
<400> 19
atggcggaag ttctggtgag cgcatccaca ggggcaatgg gttccctcct gcggaagttg 60
ggagctatgt tgacggatga atacaagctg ctcaagaacg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgcg ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacacgggt cctcctgttc cgaggcggct 300
catggcgtcg ccaagctcat ggacaagtgc aagaacttgc tgccggacat caaggcccgc 360
cgcaggatcg ccaaggaggt caaagacatc aagaaagaaa ttaaggatgt cagcgacaga 420
ttttcaaggt accagatctt gttctcctta cagtactatt agtacctacc atcagatttg 480
tgaccgattt gttgccactc aaggtacaag attgacgact cctcctcctc tatgccggca 540
aaagacaagg tcgaccctcg acttcgtgca gtctacaaag acgccgcaga gctcgtcgga 600
attgatggac caaaggatga acttgtcaag tggctgaatg agaaagaagg ccaatcactg 660
aaatctgtct ctattgttgg atatggaggg ttgggcaaga ccacgctcgc caaccagatc 720
cgggtcaacc ttggagcgac cttcgactgc ggggcttttg tctcgatttc acgcaagcct 780
gacatgaagg cgatattaag atctatatta tcacaaatca ccaagaaaga cgatgcttgc 840
tctagattag atgatataca actcatcatc gacaagattc gagaattcct ccaagacaca 900
aggtatgtat gtgagatctg ctacgaagta acattgctta attagctcat gcataatcgg 960
ttgcctagaa ttttgctaat gaagattgct ttgtgtgatt tactttcgtc ctcaacttgt 1020
atgactgcgc ttaaactttc aagtcattgt ttcttgcaac ttatgtcgat ctgttcacta 1080
attaagatat agctaaaatg tgtttgccga tttctgtcgc cacacatttt ctaatttgat 1140
tgccacaagt ttgttaaact tgcggctatg ttctgtttat taaataattg tgacatggcg 1200
tcagtcaaaa caataaagta catgcaattc cctctctttt gtacaagata aatatccgca 1260
cgcactacat gactgcaaat atatctatct tctaaggacc caaagcactt gcatgtgcag 1320
accattgtta ccggatatgc agtacattag gtttgtgatt agccacttta agaacatagg 1380
gtttctagag gctataaacc tacggttatc agtattataa tcttgaatgg atatcaaaca 1440
tccgttgaag aatctatgcc cataacacaa aaagagaaaa gggaagaatt aatcatagtc 1500
agatcggacc agcctgacta gcgtaacata atgacaacag taggttgttg cgtctttcct 1560
ggtttcgtag caatttgcat gacgaattga ggcgcagagc caccactcaa tatgtggtcc 1620
taccaatcat gaggaaataa cttgttacca aaatgacaaa agaaaatagg tagtccccat 1680
cattgtttca atatctatcc tcttgcgaac atgctattgg cagtggcaaa acattgtacc 1740
ttgccactgg caacagctgc ctttactcgg cctgagcatt catatttttt tcctcttggt 1800
tgttaaatct tgctattttt cgcttgcctt ctatggcaat cttttttttt cttatattgc 1860
caccaaattg tatctaagag caactccaac acgccgacgc aatttttgtc catttagatc 1920
ggccaagcgg acggggtgtc tggttttgcc tttgggtggt gcgcctacgc attagtccgc 1980
gtgggcattt aaaaaactta aaaagcttaa aataattaaa tataaataaa aatgcatatt 2040
aaagcccgtc aaagtccaca taattaaaca taatacatta atatattaat aaaaaacagc 2100
cgccccagcc cgcagcccta ggcgtcttcg tcggaggagt gggtgaggtc gaagaatgcc 2160
ggcagctcag cccacgggaa ggcctgctgg tgggcagcca gcaccgcctg ctccgctgtc 2220
tgcggcggcg gcggccctac ctggcaccgc gcacgcctcc tcctcgcgct cctgccgctc 2280
ctcctcctca tcgcacgccc aacgaagttt tggcgctcct ccccctcggg ttcccgctgc 2340
atgcggacct ggccgtcgta ccgctcctcc tccagcgtgc ggtccttcca cgccacgagg 2400
taggccgcct cctcctccat cacgcacagc caaatgggcg gcttgctcat ccgcttgcag 2460
acgatgccct gccattggta aacgccggtg ggcgcgggtg gtgctagcgc tggtgcaggc 2520
ggtgacgatg tggctggtgg tcccggcagg tagacactat agctggcggc cgatagctgc 2580
agcgtgtcat cgaggccaga ccactaggag tcctcgcact aagactcctc ggtgacgtga 2640
tgggccccct cctccaatgc cttgaggaac gccgcctcct cctcaaccta ctatttgacc 2700
accggtggcg gcggtggagg gcaggcttcg agctggacgg cgtcgcggag gcgcacatgg 2760
tgctcgatgg tgaaccagac ttctcagtta ggggagtctg gcctcgatgg gtggagtcgc 2820
gacgctgctc tggcgtcagg agagcacgcc gggcccgaat ctcgttggcg cgcgcacgct 2880
cggactgtgg caccgccggc actggaatgt ggtgggggtc gaggtgccag ccgtggggga 2940
agttcacgtc cggccacggc agtgagacgc ggtactcccg gtggtactac gcgtggtgca 3000
ccgggatgta ctggtggtcc cgtggcgctg ccgccctccc atcccgcagc gccgacacca 3060
atgcgaaccc cttcgctgcc ggcctcacca gcccttgcaa cgagccggcc tcgtggtcgt 3120
gcttgccttg cccttacctt tgccaaagaa acccatgacg gctagggttt tctgtcaccg 3180
gcgagggagc agatgtggac tagagtggga ggagaagtgg atgaggcccg ccccgccgca 3240
cccttgcaat taaaaagggc gtgatccgga cgtttctagt cactgccatg cgggcccgag 3300
gtaggtgggc gccattgact acgtctgtgg tggtagttgg ctggcagaca cgccagcccg 3360
aggcgcacac cgagcggacg cgcaacacgt ccgtctcgtg ttcgtgtgaa cgcaaatcag 3420
gcacaaattt gggctggaaa tgcgtctaca tggatgccga acggacgtga tttgggtttg 3480
tgtccgcacg ttgggccggc gtttttgtgc ctttcgaccc taatggacac acacggacca 3540
aatccgttgg cgtgttagag ttgcttgtac ctagtactac tatgtgtgga gttgttatct 3600
ttgagtttct cccccatctg tagctgtgta ttattgtgag atgctctcag gatttcactt 3660
attttagata atgacaactt tcttgatgct agacaattac atcaaggtgt attatatcta 3720
ggatgcacac agatgtgaga ttgtttcagg attttaggtt acttggtagg tgttactata 3780
aatatctaat gagctctaat gtgtatgttc atgaaactgt gttgcaggta ctttatcata 3840
attgatgata tatgggagtt aggaacatgg gaaactttga aatgtgcatt tgtcaagaat 3900
acattgggca gcagaataat tatcacaaca cgtatagttg atgttgccaa atcttgttct 3960
ccttctagtg aagatcttgt ctatgagatg aaaccactca gtgaggctga ctcgaagaaa 4020
ttgtttttca agaggatatt tggctgtgaa gaaagctgtc ctgatagctt gaaagaagct 4080
gccaatgata ttttgaaaaa atgccgtggc ctacctctgg ccatcaatgc catttctagc 4140
gtgttggtca caacaaggga aacaaaagaa gagtgggatc gggtgcgaca ttcaatacgc 4200
tcttcaaagg tcaaaagtga tataatagag accatgaatt acatattgtc tcttagttat 4260
tttgatcttc cccaccacct aagaagctgc ttattgtacc tggctctgtt tcctgaagac 4320
caattgattg gaaggaagcg gttagtacgc agatggattt ctgaaggctt tatccatgga 4380
gaaagtggac aagatctcat ggaattagga gaggaatatt ttcaccagct tgtgaacaga 4440
agtctaatac agcccggcaa catagggtat gacggcaagg caatgtattg ccgagtccat 4500
gacaccatcc ttgatttcct aattgacaaa tcctctgaag agaacatgtg tactgtccta 4560
aagaaacaat gcaagcctaa tggcatagtt cgtcggctct ctctaatggg gaatgaggat 4620
gaagaaatcg tggagcaatt ggatctgtca cacgctcggt caatcactgc ttttggggat 4680
atcaagctat tgccttcgct tgggaggtca aaatgcttgc gcgtgctaga cttgcaagac 4740
tgcgatcagt tggaaaatca tcatatcaag gatattgaaa gactctatca gctaagatac 4800
ttggatatct cttctacagg aatcacggag cttcccaggc aaattgggga gttgctgtat 4860
ctagagacgc tagttgccta tggattacgt gagcttcctg aaagcacaag tcggctacaa 4920
cgactggcac gtttgtttgt ttactctggt tgtaagcttc caggtgggtt gggaaacttg 4980
ataaacttgc aagagcttga ctgcgttgat gccttgcatt tgaagcatgt ggaagagctc 5040
ggcaaactaa caaatctgag aaagctgagt attaaattgg acactggtgg gattgaaggc 5100
aacaaattag aagagtccaa ggaaaagctg gtgtcctctc tctgtaaact ggacgaatgc 5160
ggcctccttt ccctgagtat tgattattat ctgagagaaa aggatggaga agagccgttc 5220
ctccctgctt tgggttgtat ccaggaggtt tttgtatatg gacaagatat cagccggatt 5280
agcagatggc tcgcgtcact tcccaaccta cacaggttgc ttttggacga tccgaagata 5340
gagcagcagg atattgagat gattggattg atacccaatc tgatcgattt gacactgccg 5400
cctctataca aaacagacga cgctgggcgg ctcatcatca ggcgtgaagg atttcaacag 5460
ttgcagaagt ttgaggctta caatactcgt atgggggttt taatgtttga accgggggct 5520
atgccgaggc tcaaggagct taaactccat aacttcatcg aaaagcccaa atctgctgcg 5580
gtcgatttcg actttggcat ccaacgcctc tccagccttg ctcgcctcac tgtcagctta 5640
tcctgcggtg gctggacggt ggcagaagta gaggctgcgg aggatgcttt caagagcatg 5700
gccgaggcga accccaaccg tccaatactg gagatgacaa gatataatac gcagcatatg 5760
ttacaagacg agcaaatagg tatgacaggc tcaggtacta ttaactagtt tgtacttttg 5820
tcttggtgag cagtagatca aaagatgtgc atatgcttca atcttttttc cggtaatcta 5880
tttcagctac aacgccggcc gtcaagtcct ag 5912
<210> 20
<211> 939
<212> PRT
<213> 野生一粒小麦
<400> 20
Met Ala Glu Val Leu Val Ser Ala Ser Thr Gly Ala Met Gly Ser Leu
1 5 10 15
Leu Arg Lys Leu Gly Ala Met Leu Thr Asp Glu Tyr Lys Leu Leu Lys
20 25 30
Asn Val Arg Gly Asp Ile Lys Phe Leu Lys Asp Glu Leu Glu Val Met
35 40 45
Cys Ala Phe Leu Leu Lys Met Ser Asp Val Glu Glu Pro Asp Glu Pro
50 55 60
Thr Lys Leu Arg Val Thr Ala Val Arg Glu Met Ser Tyr Lys Ile Glu
65 70 75 80
Asp Asn Ile Asp Lys Phe Met Val Leu Val Glu His Gly Ser Ser Cys
85 90 95
Ser Glu Ala Ala His Gly Val Ala Lys Leu Met Asp Lys Cys Lys Asn
100 105 110
Leu Leu Pro Asp Ile Lys Ala Arg Arg Arg Ile Ala Lys Glu Val Lys
115 120 125
Asp Ile Lys Lys Glu Ile Lys Asp Val Ser Asp Arg Phe Ser Arg Tyr
130 135 140
Lys Ile Asp Asp Ser Ser Ser Ser Met Pro Ala Lys Asp Lys Val Asp
145 150 155 160
Pro Arg Leu Arg Ala Val Tyr Lys Asp Ala Ala Glu Leu Val Gly Ile
165 170 175
Asp Gly Pro Lys Asp Glu Leu Val Lys Trp Leu Asn Glu Lys Glu Gly
180 185 190
Gln Ser Leu Lys Ser Val Ser Ile Val Gly Tyr Gly Gly Leu Gly Lys
195 200 205
Thr Thr Leu Ala Asn Gln Ile Arg Val Asn Leu Gly Ala Thr Phe Asp
210 215 220
Cys Gly Ala Phe Val Ser Ile Ser Arg Lys Pro Asp Met Lys Ala Ile
225 230 235 240
Leu Arg Ser Ile Leu Ser Gln Ile Thr Lys Lys Asp Asp Ala Cys Ser
245 250 255
Arg Leu Asp Asp Ile Gln Leu Ile Ile Asp Lys Ile Arg Glu Phe Leu
260 265 270
Gln Asp Thr Arg Tyr Phe Ile Ile Ile Asp Asp Ile Trp Glu Leu Gly
275 280 285
Thr Trp Glu Thr Leu Lys Cys Ala Phe Val Lys Asn Thr Leu Gly Ser
290 295 300
Arg Ile Ile Ile Thr Thr Arg Ile Val Asp Val Ala Lys Ser Cys Ser
305 310 315 320
Pro Ser Ser Glu Asp Leu Val Tyr Glu Met Lys Pro Leu Ser Glu Ala
325 330 335
Asp Ser Lys Lys Leu Phe Phe Lys Arg Ile Phe Gly Cys Glu Glu Ser
340 345 350
Cys Pro Asp Ser Leu Lys Glu Ala Ala Asn Asp Ile Leu Lys Lys Cys
355 360 365
Arg Gly Leu Pro Leu Ala Ile Asn Ala Ile Ser Ser Val Leu Val Thr
370 375 380
Thr Arg Glu Thr Lys Glu Glu Trp Asp Arg Val Arg His Ser Ile Arg
385 390 395 400
Ser Ser Lys Val Lys Ser Asp Ile Ile Glu Thr Met Asn Tyr Ile Leu
405 410 415
Ser Leu Ser Tyr Phe Asp Leu Pro His His Leu Arg Ser Cys Leu Leu
420 425 430
Tyr Leu Ala Leu Phe Pro Glu Asp Gln Leu Ile Gly Arg Lys Arg Leu
435 440 445
Val Arg Arg Trp Ile Ser Glu Gly Phe Ile His Gly Glu Ser Gly Gln
450 455 460
Asp Leu Met Glu Leu Gly Glu Glu Tyr Phe His Gln Leu Val Asn Arg
465 470 475 480
Ser Leu Ile Gln Pro Gly Asn Ile Gly Tyr Asp Gly Lys Ala Met Tyr
485 490 495
Cys Arg Val His Asp Thr Ile Leu Asp Phe Leu Ile Asp Lys Ser Ser
500 505 510
Glu Glu Asn Met Cys Thr Val Leu Lys Lys Gln Cys Lys Pro Asn Gly
515 520 525
Ile Val Arg Arg Leu Ser Leu Met Gly Asn Glu Asp Glu Glu Ile Val
530 535 540
Glu Gln Leu Asp Leu Ser His Ala Arg Ser Ile Thr Ala Phe Gly Asp
545 550 555 560
Ile Lys Leu Leu Pro Ser Leu Gly Arg Ser Lys Cys Leu Arg Val Leu
565 570 575
Asp Leu Gln Asp Cys Asp Gln Leu Glu Asn His His Ile Lys Asp Ile
580 585 590
Glu Arg Leu Tyr Gln Leu Arg Tyr Leu Asp Ile Ser Ser Thr Gly Ile
595 600 605
Thr Glu Leu Pro Arg Gln Ile Gly Glu Leu Leu Tyr Leu Glu Thr Leu
610 615 620
Val Ala Tyr Gly Leu Arg Glu Leu Pro Glu Ser Thr Ser Arg Leu Gln
625 630 635 640
Arg Leu Ala Arg Leu Phe Val Tyr Ser Gly Cys Lys Leu Pro Gly Gly
645 650 655
Leu Gly Asn Leu Ile Asn Leu Gln Glu Leu Asp Cys Val Asp Ala Leu
660 665 670
His Leu Lys His Val Glu Glu Leu Gly Lys Leu Thr Asn Leu Arg Lys
675 680 685
Leu Ser Ile Lys Leu Asp Thr Gly Gly Ile Glu Gly Asn Lys Leu Glu
690 695 700
Glu Ser Lys Glu Lys Leu Val Ser Ser Leu Cys Lys Leu Asp Glu Cys
705 710 715 720
Gly Leu Leu Ser Leu Ser Ile Asp Tyr Tyr Leu Arg Glu Lys Asp Gly
725 730 735
Glu Glu Pro Phe Leu Pro Ala Leu Gly Cys Ile Gln Glu Val Phe Val
740 745 750
Tyr Gly Gln Asp Ile Ser Arg Ile Ser Arg Trp Leu Ala Ser Leu Pro
755 760 765
Asn Leu His Arg Leu Leu Leu Asp Asp Pro Lys Ile Glu Gln Gln Asp
770 775 780
Ile Glu Met Ile Gly Leu Ile Pro Asn Leu Ile Asp Leu Thr Leu Pro
785 790 795 800
Pro Leu Tyr Lys Thr Asp Asp Ala Gly Arg Leu Ile Ile Arg Arg Glu
805 810 815
Gly Phe Gln Gln Leu Gln Lys Phe Glu Ala Tyr Asn Thr Arg Met Gly
820 825 830
Val Leu Met Phe Glu Pro Gly Ala Met Pro Arg Leu Lys Glu Leu Lys
835 840 845
Leu His Asn Phe Ile Glu Lys Pro Lys Ser Ala Ala Val Asp Phe Asp
850 855 860
Phe Gly Ile Gln Arg Leu Ser Ser Leu Ala Arg Leu Thr Val Ser Leu
865 870 875 880
Ser Cys Gly Gly Trp Thr Val Ala Glu Val Glu Ala Ala Glu Asp Ala
885 890 895
Phe Lys Ser Met Ala Glu Ala Asn Pro Asn Arg Pro Ile Leu Glu Met
900 905 910
Thr Arg Tyr Asn Thr Gln His Met Leu Gln Asp Glu Gln Ile Gly Met
915 920 925
Thr Gly Ser Ala Thr Thr Pro Ala Val Lys Ser
930 935
<210> 21
<211> 2817
<212> DNA
<213> 野生一粒小麦
<400> 21
atggcggaag ttctggtgag cgcatccaca ggggcaatgg gttccctcct gcggaagttg 60
ggagctatgt tgacggatga atacaagctg ctcaagaacg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgcg ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacacgggt cctcctgttc cgaggcggct 300
catggcgtcg ccaagctcat ggacaagtgc aagaacttgc tgccggacat caaggcccgc 360
cgcaggatcg ccaaggaggt caaagacatc aagaaagaaa ttaaggatgt cagcgacaga 420
ttttcaaggt acaagattga cgactcctcc tcctctatgc cggcaaaaga caaggtcgac 480
cctcgacttc gtgcagtcta caaagacgcc gcagagctcg tcggaattga tggaccaaag 540
gatgaacttg tcaagtggct gaatgagaaa gaaggccaat cactgaaatc tgtctctatt 600
gttggatatg gagggttggg caagaccacg ctcgccaacc agatccgggt caaccttgga 660
gcgaccttcg actgcggggc ttttgtctcg atttcacgca agcctgacat gaaggcgata 720
ttaagatcta tattatcaca aatcaccaag aaagacgatg cttgctctag attagatgat 780
atacaactca tcatcgacaa gattcgagaa ttcctccaag acacaaggta ctttatcata 840
attgatgata tatgggagtt aggaacatgg gaaactttga aatgtgcatt tgtcaagaat 900
acattgggca gcagaataat tatcacaaca cgtatagttg atgttgccaa atcttgttct 960
ccttctagtg aagatcttgt ctatgagatg aaaccactca gtgaggctga ctcgaagaaa 1020
ttgtttttca agaggatatt tggctgtgaa gaaagctgtc ctgatagctt gaaagaagct 1080
gccaatgata ttttgaaaaa atgccgtggc ctacctctgg ccatcaatgc catttctagc 1140
gtgttggtca caacaaggga aacaaaagaa gagtgggatc gggtgcgaca ttcaatacgc 1200
tcttcaaagg tcaaaagtga tataatagag accatgaatt acatattgtc tcttagttat 1260
tttgatcttc cccaccacct aagaagctgc ttattgtacc tggctctgtt tcctgaagac 1320
caattgattg gaaggaagcg gttagtacgc agatggattt ctgaaggctt tatccatgga 1380
gaaagtggac aagatctcat ggaattagga gaggaatatt ttcaccagct tgtgaacaga 1440
agtctaatac agcccggcaa catagggtat gacggcaagg caatgtattg ccgagtccat 1500
gacaccatcc ttgatttcct aattgacaaa tcctctgaag agaacatgtg tactgtccta 1560
aagaaacaat gcaagcctaa tggcatagtt cgtcggctct ctctaatggg gaatgaggat 1620
gaagaaatcg tggagcaatt ggatctgtca cacgctcggt caatcactgc ttttggggat 1680
atcaagctat tgccttcgct tgggaggtca aaatgcttgc gcgtgctaga cttgcaagac 1740
tgcgatcagt tggaaaatca tcatatcaag gatattgaaa gactctatca gctaagatac 1800
ttggatatct cttctacagg aatcacggag cttcccaggc aaattgggga gttgctgtat 1860
ctagagacgc tagttgccta tggattacgt gagcttcctg aaagcacaag tcggctacaa 1920
cgactggcac gtttgtttgt ttactctggt tgtaagcttc caggtgggtt gggaaacttg 1980
ataaacttgc aagagcttga ctgcgttgat gccttgcatt tgaagcatgt ggaagagctc 2040
ggcaaactaa caaatctgag aaagctgagt attaaattgg acactggtgg gattgaaggc 2100
aacaaattag aagagtccaa ggaaaagctg gtgtcctctc tctgtaaact ggacgaatgc 2160
ggcctccttt ccctgagtat tgattattat ctgagagaaa aggatggaga agagccgttc 2220
ctccctgctt tgggttgtat ccaggaggtt tttgtatatg gacaagatat cagccggatt 2280
agcagatggc tcgcgtcact tcccaaccta cacaggttgc ttttggacga tccgaagata 2340
gagcagcagg atattgagat gattggattg atacccaatc tgatcgattt gacactgccg 2400
cctctataca aaacagacga cgctgggcgg ctcatcatca ggcgtgaagg atttcaacag 2460
ttgcagaagt ttgaggctta caatactcgt atgggggttt taatgtttga accgggggct 2520
atgccgaggc tcaaggagct taaactccat aacttcatcg aaaagcccaa atctgctgcg 2580
gtcgatttcg actttggcat ccaacgcctc tccagccttg ctcgcctcac tgtcagctta 2640
tcctgcggtg gctggacggt ggcagaagta gaggctgcgg aggatgcttt caagagcatg 2700
gccgaggcga accccaaccg tccaatactg gagatgacaa gatataatac gcagcatatg 2760
ttacaagacg agcaaatagg tatgacaggc tcagctacaa cgccggccgt caagtcc 2817
<210> 22
<211> 5912
<212> DNA
<213> 野生一粒小麦
<400> 22
atggcggaag ttctggtgag cgcatccaca ggggcaatgg gttccctcct gcggaagttg 60
ggagctatgt tgacggatga atacaagctg ctcaagaacg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgcg ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacacgagt cctcctgctc cgaggcggct 300
catggcgtcg ccaagctcat ggacaagtgc aagaacttgc tgccggacat caagacccgc 360
cgcaggatcg ccaaggaggt caaagacatc aagaaagaaa ttaaggatgt cagcgacaga 420
ttttcaaggt accagatctt gttctcttta cagtactatt agtacctacc atcagatttg 480
tgaccgattt gttgccactc aaggtacaag attgacgagt cctcctcctc tatgccggca 540
aaaggcaagg tcgaccctcg acttcgtgca gtctacaaag acgccgcaga gctcgtcgga 600
attgatggac caaaggatga acttgtcaag tggctgaatg agaaagaggg ccaatcactg 660
aaatctgtct ctattgttgg atatggaggg ttgggcaaga ccacgctcgc caaccagatc 720
cgggtcaacc ttggagcgac cttcgactgc ggggcttttg tctcgatttc acgcaagcct 780
gacatgaagg cgatattaag atctatatta tcgcaaatca ccaagaaaga cgaggcttgc 840
tctagattag atgatataca actcatcatc gacaagattc gagaattcct ccaagacaca 900
aggtatgtat gtgagatcta ctacgaagta acattgctta attagctcat gcataatccg 960
ttgcctagaa ttttgctaat gaagattgct ttgtgtgatt tactttcgtc ctcaacttgt 1020
atgactgcgc ttaaactttc aagtcattgt ttcttgcaac ttatgtcgat ctgttcacta 1080
attaagatat agctaaaatg tgtttgccga tttctgtcgc cacacatttt ctaatttgat 1140
tgccacaagt ttgttaaact tgcggctatg ttctgtttat taaataattg tgacatggcg 1200
tcagtcaaaa caataaagta catgcaattc cctctctttt gtacaagata aatatccgca 1260
cgcactacat gactgcaaat atatctatct tctaaggacc caaagcactt gcatgtgcag 1320
accattgtta ccggatatgc agtacattag gtttgtgatt agccacttta agaacatagg 1380
gtttctagag gctataaacc tacggttatc agtattataa tcttgaatgg atatcaaaca 1440
tccgttgaag aatctatgcc cataacacaa aaagagaaaa gggaagaatt aatcatagtc 1500
agatcggacc agcctgacta gcgtaacata atgacaacag taggttgttg cgtctttcct 1560
ggtttcgtag caatttgcat gacgaattga ggcgcagagc caccactcaa tatgtggtcc 1620
taccaatcat gaggaaataa cttgttacca aaatgacaaa agaaaatagg tagtccccat 1680
cattgtttca atatctatcc tcttgcgaac atgctattgg cagtggcaaa acattgtacc 1740
ttgccactgg caacagctgc ctttactcgg cctgagcatt catatttttt tcctcttggt 1800
tgttaaatct tgctattttt cgcttgcctt ctatggcaat cttttttttt cttatattgc 1860
caccaaattg tatctaagag caactccaac acgccgacgc aatttttgtc catttagatc 1920
ggccaagcgg acggggtgtc tggttttgcc tttgggtggt gcgcctacgc attagtccgc 1980
gtgggcattt aaaaaactta aaaagcttaa aataattaaa tataaataaa aatgcatatt 2040
aaagcccgtc aaagtccaca taattaaaca taatacatta atatattaat aaaaaacagc 2100
cgccccagcc cgcagcccta ggcgtcttcg tcggaggagt gggtgaggtc gaagaatgcc 2160
ggcagctcag cccacgggaa ggcctgctgg tgggcagcca gcaccgcctg ctccgctgtc 2220
tgcggcggcg gcggccctac ctggcaccgc gcacgcctcc tcctcgcgct cctgccgctc 2280
ctcctcctca tcgcacgccc aacgaagttt tggcgctcct ccccctcggg ttcccgctgc 2340
atgcggacct ggccgtcgta ccgctcctcc tccagcgtgc ggtccttcca cgccacgagg 2400
taggccgcct cctcctccat cacgcacagc caaatgggcg gcttgctcat ccgcttgcag 2460
acgatgccct gccattggta aacgccggtg ggcgcgggtg gtgctagcgc tggtgcaggc 2520
ggtgacgatg tggctggtgg tcccggcagg tagacactat agctggcggc cgatagctgc 2580
agcgtgtcat cgaggccaga ccactaggag tcctcgcact aagactcctc ggtgacgtga 2640
tgggccccct cctccaatgc cttgaggaac gccgcctcct cctcaaccta ctatttgacc 2700
accggtggcg gcggtggagg gcaggcttcg agctggacgg cgtcgcggag gcgcacatgg 2760
tgctcgatgg tgaaccagac ttctcagtta ggggagtctg gcctcgatgg gtggagtcgc 2820
gacgctgctc tggcgtcagg agagcacgcc gggcccgaat ctcgttggcg cgcgcacgct 2880
cggactgtgg caccgccggc actggaatgt ggtgggggtc gaggtgccag ccgtggggga 2940
agttcacgtc cggccacggc agtgagacgc ggtactcccg gtggtactac gcgtggtgca 3000
ccgggatgta ctggtggtcc cgtggcgctg ccgccctccc atcccgcagc gccgacacca 3060
atgcgaaccc cttcgctgcc ggcctcaccg gcccttgcaa cgagccggcc tcgtggtcgt 3120
gcttgccttg cccttacctt tgccaaagaa acccatgacg gctagggttt tctgtcaccg 3180
gcaagggagc agatgtggac tagagtggga ggagaagtgg atgaggcccg ccccgccgca 3240
cccttgcaat taaaaagggc gtgatccgga cgtttctagt cactgccatg cgggcccgag 3300
gtaggtgggc gccattgact acgtctgtgg tggtagttgg ctggcagaca cgccagcccg 3360
aggcgcacac cgagcggacg cgcaacacgt ccgtctcgtg ttcgtgtgaa cgcaaatcac 3420
gcacaaattt gggctggaaa tgcgtctaca tggatgccga acggacgtga tttgggtttg 3480
tgtccgcacg ttgggccggc gtttttgtgc ctttcgaccc taatggacac acacggacca 3540
aatccgttgg cgtgttagag ttgcttgtac ctagtactac tatgtgtgga gttgttatct 3600
ttgagtttct cccccatctg tagctgtgta ttattgtgag atgctctcag gatttcactt 3660
attttagata atgacaactt tcttgatgct agacaattac atcaaggtgt attatatcta 3720
ggatgcacac agatgtgaga ttgtttcagg attttaggtt acttggtagg tgttactata 3780
aatatctaat gagctctaat gtgtatgttc atgaaactgt gttgcaggta ctttatcata 3840
attgatgata tatgggagtt aggaacatgg gaaactttga aatgtgcatt tgtcaagaat 3900
acattgggca gcagaataat tatcacaaca cgtatagttg atgttgccaa atcttgttct 3960
ccttctagtg aagatcttgt ctatgagatg aaaccactca gtgaggctga ctcgaagaaa 4020
ttgtttttca agaggatatt tggctgtgaa gaaagctgtc ctgatagctt gaaagaagct 4080
gccaatgata ttttgaaaaa atgccgtggc ctacctctgg ccatcaatgc catttctagc 4140
gtgttggtca caacaaggga aacaaaagaa gagtgggatc gggtgcgaca ttcaatacgc 4200
tcttcaaagg tcaaaagtga tataatagag accatgaatt acatattgtc tcttagttat 4260
tttgatcttc cccaccacct aagaagctgc ttattgtacc tggctctgtt tcctgaagac 4320
caattgattg gaaggaagcg gttagtacgc agatggattt ctgaaggctt tatccatgga 4380
gaaagtggac aagatctcat ggaattagga gaggaatatt ttcaccagct tgtgaacaga 4440
agtctaatac agcccggcaa catagggtat gacggcaagg caatgtattg ccgagtccat 4500
gacaccatcc ttgatttcct aattgacaaa tcctctgaag agaacatgtg tactgtccta 4560
aagaaacaat gcaagcctaa tggcatagtt cgtcggctct ctctaatggg gaatgaggat 4620
gaagaaatcg tggagcaatt ggatctgtca cacgctcggt caatcactgc ttttggggat 4680
atcaagctat tgccttcgct tgggaggtca aaatgcttgc gcgtgctaga cttgcaagac 4740
tgcgatcagt tggaaaatca tcatatcaag gatattgaaa gactctatca gctaagatac 4800
ttggatatct cttctacagg aatcacggag cttcccaggc aaattgggga gttgctgtat 4860
ctagagacgc tagttgccta tggattacgt gagcttcctg aaagcacaag tcggctacaa 4920
cgactggcac gtttgtttgt ttactctggt tgtaagcttc caggtgggtt gggaaacttg 4980
ataaacttgc aagagcttga ctgcgttgat gccttgcatt tgaagcatgt ggaagagctc 5040
ggcaaactaa caaatctgag aaagctgagt attaaattgg acactggtgg gattgaaggc 5100
aacaaattag aagagtccaa ggaaaagctg gtgtcctctc tctgtaaact ggacgaatgc 5160
ggcctccttt ccctgagtat tgattattat ctgagagaaa aggatggaga agagccgttc 5220
ctccctgctt tgggttgtat ccaggaggtt tttgtatatg gacaagatat cagccggatt 5280
agcagatggc tcgcgtcact tcccaaccta cacaggttgc ttttggacga tccgaagata 5340
gagcagcagg atattgagat gattggattg atacccaatc tgatcgattt gacactgccg 5400
cctctataca aaacagacga cgctgggcgg ctcatcatca ggcgtgaagg atttcaacag 5460
ttgcagaagt ttgaggctta caatactcgt atgggggttt taatgtttga accgggggct 5520
atgccgaggc tcaaggagct taaactccat aacttcatcg aaaagcccaa atctgctgcg 5580
gtcgatttcg actttggcat ccaacgcctc tccagccttg ctcgcctcac tgtcagctta 5640
tcctgcggtg gctggacggt ggcagaagta gaggctgcgg aggatgcttt caagagcatg 5700
gccgaggcga accccaaccg tccaatactg gagatgacaa gatataatac gcagcatatg 5760
ttacaagacg agcaaatagg tatgacaggc tcaggtacta ttaactagtt tgtacttttg 5820
tcttggtgag cagtagatca aaagatgtgc atatgcttca atcttttttc cggtaatcta 5880
tttcagctac aacgccggcc gtcaagtcct ag 5912
<210> 23
<211> 939
<212> PRT
<213> 野生一粒小麦
<400> 23
Met Ala Glu Val Leu Val Ser Ala Ser Thr Gly Ala Met Gly Ser Leu
1 5 10 15
Leu Arg Lys Leu Gly Ala Met Leu Thr Asp Glu Tyr Lys Leu Leu Lys
20 25 30
Asn Val Arg Gly Asp Ile Lys Phe Leu Lys Asp Glu Leu Glu Val Met
35 40 45
Cys Ala Phe Leu Leu Lys Met Ser Asp Val Glu Glu Pro Asp Glu Pro
50 55 60
Thr Lys Leu Arg Val Thr Ala Val Arg Glu Met Ser Tyr Lys Ile Glu
65 70 75 80
Asp Asn Ile Asp Lys Phe Met Val Leu Val Glu His Glu Ser Ser Cys
85 90 95
Ser Glu Ala Ala His Gly Val Ala Lys Leu Met Asp Lys Cys Lys Asn
100 105 110
Leu Leu Pro Asp Ile Lys Thr Arg Arg Arg Ile Ala Lys Glu Val Lys
115 120 125
Asp Ile Lys Lys Glu Ile Lys Asp Val Ser Asp Arg Phe Ser Arg Tyr
130 135 140
Lys Ile Asp Glu Ser Ser Ser Ser Met Pro Ala Lys Gly Lys Val Asp
145 150 155 160
Pro Arg Leu Arg Ala Val Tyr Lys Asp Ala Ala Glu Leu Val Gly Ile
165 170 175
Asp Gly Pro Lys Asp Glu Leu Val Lys Trp Leu Asn Glu Lys Glu Gly
180 185 190
Gln Ser Leu Lys Ser Val Ser Ile Val Gly Tyr Gly Gly Leu Gly Lys
195 200 205
Thr Thr Leu Ala Asn Gln Ile Arg Val Asn Leu Gly Ala Thr Phe Asp
210 215 220
Cys Gly Ala Phe Val Ser Ile Ser Arg Lys Pro Asp Met Lys Ala Ile
225 230 235 240
Leu Arg Ser Ile Leu Ser Gln Ile Thr Lys Lys Asp Glu Ala Cys Ser
245 250 255
Arg Leu Asp Asp Ile Gln Leu Ile Ile Asp Lys Ile Arg Glu Phe Leu
260 265 270
Gln Asp Thr Arg Tyr Phe Ile Ile Ile Asp Asp Ile Trp Glu Leu Gly
275 280 285
Thr Trp Glu Thr Leu Lys Cys Ala Phe Val Lys Asn Thr Leu Gly Ser
290 295 300
Arg Ile Ile Ile Thr Thr Arg Ile Val Asp Val Ala Lys Ser Cys Ser
305 310 315 320
Pro Ser Ser Glu Asp Leu Val Tyr Glu Met Lys Pro Leu Ser Glu Ala
325 330 335
Asp Ser Lys Lys Leu Phe Phe Lys Arg Ile Phe Gly Cys Glu Glu Ser
340 345 350
Cys Pro Asp Ser Leu Lys Glu Ala Ala Asn Asp Ile Leu Lys Lys Cys
355 360 365
Arg Gly Leu Pro Leu Ala Ile Asn Ala Ile Ser Ser Val Leu Val Thr
370 375 380
Thr Arg Glu Thr Lys Glu Glu Trp Asp Arg Val Arg His Ser Ile Arg
385 390 395 400
Ser Ser Lys Val Lys Ser Asp Ile Ile Glu Thr Met Asn Tyr Ile Leu
405 410 415
Ser Leu Ser Tyr Phe Asp Leu Pro His His Leu Arg Ser Cys Leu Leu
420 425 430
Tyr Leu Ala Leu Phe Pro Glu Asp Gln Leu Ile Gly Arg Lys Arg Leu
435 440 445
Val Arg Arg Trp Ile Ser Glu Gly Phe Ile His Gly Glu Ser Gly Gln
450 455 460
Asp Leu Met Glu Leu Gly Glu Glu Tyr Phe His Gln Leu Val Asn Arg
465 470 475 480
Ser Leu Ile Gln Pro Gly Asn Ile Gly Tyr Asp Gly Lys Ala Met Tyr
485 490 495
Cys Arg Val His Asp Thr Ile Leu Asp Phe Leu Ile Asp Lys Ser Ser
500 505 510
Glu Glu Asn Met Cys Thr Val Leu Lys Lys Gln Cys Lys Pro Asn Gly
515 520 525
Ile Val Arg Arg Leu Ser Leu Met Gly Asn Glu Asp Glu Glu Ile Val
530 535 540
Glu Gln Leu Asp Leu Ser His Ala Arg Ser Ile Thr Ala Phe Gly Asp
545 550 555 560
Ile Lys Leu Leu Pro Ser Leu Gly Arg Ser Lys Cys Leu Arg Val Leu
565 570 575
Asp Leu Gln Asp Cys Asp Gln Leu Glu Asn His His Ile Lys Asp Ile
580 585 590
Glu Arg Leu Tyr Gln Leu Arg Tyr Leu Asp Ile Ser Ser Thr Gly Ile
595 600 605
Thr Glu Leu Pro Arg Gln Ile Gly Glu Leu Leu Tyr Leu Glu Thr Leu
610 615 620
Val Ala Tyr Gly Leu Arg Glu Leu Pro Glu Ser Thr Ser Arg Leu Gln
625 630 635 640
Arg Leu Ala Arg Leu Phe Val Tyr Ser Gly Cys Lys Leu Pro Gly Gly
645 650 655
Leu Gly Asn Leu Ile Asn Leu Gln Glu Leu Asp Cys Val Asp Ala Leu
660 665 670
His Leu Lys His Val Glu Glu Leu Gly Lys Leu Thr Asn Leu Arg Lys
675 680 685
Leu Ser Ile Lys Leu Asp Thr Gly Gly Ile Glu Gly Asn Lys Leu Glu
690 695 700
Glu Ser Lys Glu Lys Leu Val Ser Ser Leu Cys Lys Leu Asp Glu Cys
705 710 715 720
Gly Leu Leu Ser Leu Ser Ile Asp Tyr Tyr Leu Arg Glu Lys Asp Gly
725 730 735
Glu Glu Pro Phe Leu Pro Ala Leu Gly Cys Ile Gln Glu Val Phe Val
740 745 750
Tyr Gly Gln Asp Ile Ser Arg Ile Ser Arg Trp Leu Ala Ser Leu Pro
755 760 765
Asn Leu His Arg Leu Leu Leu Asp Asp Pro Lys Ile Glu Gln Gln Asp
770 775 780
Ile Glu Met Ile Gly Leu Ile Pro Asn Leu Ile Asp Leu Thr Leu Pro
785 790 795 800
Pro Leu Tyr Lys Thr Asp Asp Ala Gly Arg Leu Ile Ile Arg Arg Glu
805 810 815
Gly Phe Gln Gln Leu Gln Lys Phe Glu Ala Tyr Asn Thr Arg Met Gly
820 825 830
Val Leu Met Phe Glu Pro Gly Ala Met Pro Arg Leu Lys Glu Leu Lys
835 840 845
Leu His Asn Phe Ile Glu Lys Pro Lys Ser Ala Ala Val Asp Phe Asp
850 855 860
Phe Gly Ile Gln Arg Leu Ser Ser Leu Ala Arg Leu Thr Val Ser Leu
865 870 875 880
Ser Cys Gly Gly Trp Thr Val Ala Glu Val Glu Ala Ala Glu Asp Ala
885 890 895
Phe Lys Ser Met Ala Glu Ala Asn Pro Asn Arg Pro Ile Leu Glu Met
900 905 910
Thr Arg Tyr Asn Thr Gln His Met Leu Gln Asp Glu Gln Ile Gly Met
915 920 925
Thr Gly Ser Ala Thr Thr Pro Ala Val Lys Ser
930 935
<210> 24
<211> 2817
<212> DNA
<213> 野生一粒小麦
<400> 24
atggcggaag ttctggtgag cgcatccaca ggggcaatgg gttccctcct gcggaagttg 60
ggagctatgt tgacggatga atacaagctg ctcaagaacg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgcg ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacacgagt cctcctgctc cgaggcggct 300
catggcgtcg ccaagctcat ggacaagtgc aagaacttgc tgccggacat caagacccgc 360
cgcaggatcg ccaaggaggt caaagacatc aagaaagaaa ttaaggatgt cagcgacaga 420
ttttcaaggt acaagattga cgagtcctcc tcctctatgc cggcaaaagg caaggtcgac 480
cctcgacttc gtgcagtcta caaagacgcc gcagagctcg tcggaattga tggaccaaag 540
gatgaacttg tcaagtggct gaatgagaaa gagggccaat cactgaaatc tgtctctatt 600
gttggatatg gagggttggg caagaccacg ctcgccaacc agatccgggt caaccttgga 660
gcgaccttcg actgcggggc ttttgtctcg atttcacgca agcctgacat gaaggcgata 720
ttaagatcta tattatcgca aatcaccaag aaagacgagg cttgctctag attagatgat 780
atacaactca tcatcgacaa gattcgagaa ttcctccaag acacaaggta ctttatcata 840
attgatgata tatgggagtt aggaacatgg gaaactttga aatgtgcatt tgtcaagaat 900
acattgggca gcagaataat tatcacaaca cgtatagttg atgttgccaa atcttgttct 960
ccttctagtg aagatcttgt ctatgagatg aaaccactca gtgaggctga ctcgaagaaa 1020
ttgtttttca agaggatatt tggctgtgaa gaaagctgtc ctgatagctt gaaagaagct 1080
gccaatgata ttttgaaaaa atgccgtggc ctacctctgg ccatcaatgc catttctagc 1140
gtgttggtca caacaaggga aacaaaagaa gagtgggatc gggtgcgaca ttcaatacgc 1200
tcttcaaagg tcaaaagtga tataatagag accatgaatt acatattgtc tcttagttat 1260
tttgatcttc cccaccacct aagaagctgc ttattgtacc tggctctgtt tcctgaagac 1320
caattgattg gaaggaagcg gttagtacgc agatggattt ctgaaggctt tatccatgga 1380
gaaagtggac aagatctcat ggaattagga gaggaatatt ttcaccagct tgtgaacaga 1440
agtctaatac agcccggcaa catagggtat gacggcaagg caatgtattg ccgagtccat 1500
gacaccatcc ttgatttcct aattgacaaa tcctctgaag agaacatgtg tactgtccta 1560
aagaaacaat gcaagcctaa tggcatagtt cgtcggctct ctctaatggg gaatgaggat 1620
gaagaaatcg tggagcaatt ggatctgtca cacgctcggt caatcactgc ttttggggat 1680
atcaagctat tgccttcgct tgggaggtca aaatgcttgc gcgtgctaga cttgcaagac 1740
tgcgatcagt tggaaaatca tcatatcaag gatattgaaa gactctatca gctaagatac 1800
ttggatatct cttctacagg aatcacggag cttcccaggc aaattgggga gttgctgtat 1860
ctagagacgc tagttgccta tggattacgt gagcttcctg aaagcacaag tcggctacaa 1920
cgactggcac gtttgtttgt ttactctggt tgtaagcttc caggtgggtt gggaaacttg 1980
ataaacttgc aagagcttga ctgcgttgat gccttgcatt tgaagcatgt ggaagagctc 2040
ggcaaactaa caaatctgag aaagctgagt attaaattgg acactggtgg gattgaaggc 2100
aacaaattag aagagtccaa ggaaaagctg gtgtcctctc tctgtaaact ggacgaatgc 2160
ggcctccttt ccctgagtat tgattattat ctgagagaaa aggatggaga agagccgttc 2220
ctccctgctt tgggttgtat ccaggaggtt tttgtatatg gacaagatat cagccggatt 2280
agcagatggc tcgcgtcact tcccaaccta cacaggttgc ttttggacga tccgaagata 2340
gagcagcagg atattgagat gattggattg atacccaatc tgatcgattt gacactgccg 2400
cctctataca aaacagacga cgctgggcgg ctcatcatca ggcgtgaagg atttcaacag 2460
ttgcagaagt ttgaggctta caatactcgt atgggggttt taatgtttga accgggggct 2520
atgccgaggc tcaaggagct taaactccat aacttcatcg aaaagcccaa atctgctgcg 2580
gtcgatttcg actttggcat ccaacgcctc tccagccttg ctcgcctcac tgtcagctta 2640
tcctgcggtg gctggacggt ggcagaagta gaggctgcgg aggatgcttt caagagcatg 2700
gccgaggcga accccaaccg tccaatactg gagatgacaa gatataatac gcagcatatg 2760
ttacaagacg agcaaatagg tatgacaggc tcagctacaa cgccggccgt caagtcc 2817
<210> 25
<211> 5911
<212> DNA
<213> 野生一粒小麦
<400> 25
atggcggaag ttctggtgag cgcatccaca ggggcaatgg gttccctcct gcggaagttg 60
ggagctatgt tgacggatga atacaagctg ctcaagaacg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgcg ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacacgagt cctcctgctc cgaggcggct 300
catggcgtcg ccaagctcat ggacaagtgc aagaacttgc tgccggacat caagacccgc 360
cgcaggatcg ccaaggaggt caaagacatc aagaaagaaa ttaaggatgt cagcgacaga 420
ttttcaaggt accagatctt gttctcttta cagtactatt agtacctacc atcagatttg 480
tgaccgattt gttgccactc aaggtacaag attgacgagt cctcctcctc tatgccggca 540
aaaggcaagg tcgaccctcg acttcgtgca gtctacaaag acgccgcaga gctcgtcgga 600
attgatggac caaaggatga acttgtcaag tggctgaatg agaaagaggg ccaatcactg 660
aaatctgtct ctattgttgg atatggaggg ttgggcaaga ccacgctcgc caaccagatc 720
cgggtcaacc ttggagcgac cttcgactgc ggggcttttg tctcgatttc acgcaagcct 780
gacatgaagg cgatattaag atctatatta tcgcaaatca ccaagaaaga cgaggcttgc 840
tctagattag atgatataca actcatcatc gacaagattc gagaattcct ccaagacaca 900
aggtatgtat gtgagatcta ctacgaagta acattgctta attagctcat gcataatccg 960
ttgcctagaa ttttgctaat gagattgctt tgtgtgattt actttcgtcc tcaacttgta 1020
tgactgcgct taaactttca agtcattgtt tcttgcaact tatgtcgatc tgttcactaa 1080
ttaagatata gctaaaatgt gtttgccgat ttctgtcgcc acacattttc taatttgatt 1140
gccacaagtt tgttaaactt gcggctatgt tctgtttatt aaataattgt gacatggcgt 1200
cagtcaaaac aataaagtac atgcaattcc ctctcttttg tacaagataa atatccgcac 1260
gcactacatg actgcaaata tatctatctt ctaaggaccc aaagcacttg catgtgcaga 1320
ccattgttac cggatatgca gtacattagg tttgtgatta gccactttaa gaacataggg 1380
tttctagagg ctataaacct acggttatca gtattataat cttgaatgga tatcaaacat 1440
ccgttgaaga atctatgccc ataacacaaa aagagaaaag ggaagaatta atcatagtca 1500
gatcggacca gcctgactag cgtaacataa tgacaacagt aggttgttgc gtctttcctg 1560
gtttcgtagc aatttgcatg acgaattgag gcgcagagcc accactcaat atgtggtcct 1620
accaatcatg aggaaataac ttgttaccaa aatgacaaaa gaaaataggt agtccccatc 1680
attgtttcaa tatctatcct cttgcgaaca tgctattggc agtggcaaaa cattgtacct 1740
tgccactggc aacagctgcc tttactcggc ctgagcattc atattttttt cctcttggtt 1800
gttaaatctt gctatttttc gcttgccttc tatggcaatc tttttttttc ttatattgcc 1860
accaaattgt atctaagagc aactccaaca cgccgacgca atttttgtcc atttagatcg 1920
gccaagcgga cggggtgtct ggttttgcct ttgggtggtg cgcctacgca ttagtccgcg 1980
tgggcattta aaaaacttaa aaagcttaaa ataattaaat ataaataaaa atgcatatta 2040
aagcccgtca aagtccacat aattaaacat aatacattaa tatattaata aaaaacagcc 2100
gccccagccc gcagccctag gcgtcttcgt cggaggagtg ggtgaggtcg aagaatgccg 2160
gcagctcagc ccacgggaag gcctgctggt gggcagccag caccgcctgc tccgctgtct 2220
gcggcggcgg cggccctacc tggcaccgcg cacgcctcct cctcgcgctc ctgccgctcc 2280
tcctcctcat cgcacgccca acgaagtttt ggcgctcctc cccctcgggt tcccgctgca 2340
tgcggacctg gccgtcgtac cgctcctcct ccagcgtgcg gtccttccac gccacgaggt 2400
aggccgcctc ctcctccatc acgcacagcc aaatgggcgg cttgctcatc cgcttgcaga 2460
cgatgccctg ccattggtaa acgccggtgg gcgcgggtgg tgctagcgct ggtgcaggcg 2520
gtgacgatgt ggctggtggt cccggcaggt agacactata gctggcggcc gatagctgca 2580
gcgtgtcatc gaggccagac cactaggagt cctcgcacta agactcctcg gtgacgtgat 2640
gggccccctc ctccaatgcc ttgaggaacg ccgcctcctc ctcaacctac tatttgacca 2700
ccggtggcgg cggtggaggg caggcttcga gctggacggc gtcgcggagg cgcacatggt 2760
gctcgatggt gaaccagact tctcagttag gggagtctgg cctcgatggg tggagtcgcg 2820
acgctgctct ggcgtcagga gagcacgccg ggcccgaatc tcgttggcgc gcgcacgctc 2880
ggactgtggc accgccggca ctggaatgtg gtgggggtcg aggtgccagc cgtgggggaa 2940
gttcacgtcc ggccacggca gtgagacgcg gtactcccgg tggtactacg cgtggtgcac 3000
cgggatgtac tggtggtccc gtggcgctgc cgccctccca tcccgcagcg ccgacaccaa 3060
tgcgaacccc ttcgctgccg gcctcaccgg cccttgcaac gagccggcct cgtggtcgtg 3120
cttgccttgc ccttaccttt gccaaagaaa cccatgacgg ctagggtttt ctgtcaccgg 3180
caagggagca gatgtggact agagtgggag gagaagtgga tgaggcccgc cccgccgcac 3240
ccttgcaatt aaaaagggcg tgatccggac gtttctagtc actgccatgc gggcccgagg 3300
taggtgggcg ccattgacta cgtctgtggt ggtagttggc tggcagacac gccagcccga 3360
ggcgcacacc gagcggacgc gcaacacgtc cgtctcgtgt tcgtgtgaac gcaaatcagg 3420
cacaaatttg ggctggaaat gcgtctacat ggatgccgaa cggacgtgat ttgggtttgt 3480
gtccgcacgt tgggccggcg tttttgtgcc tttcgaccct aatggacaca cacggaccaa 3540
atccgttggc gtgttagagt tgcttgtacc tagtactact atgtgtggag ttgttatctt 3600
tgagtttctc ccccatctgt agctgtgtat tattgtgaga tgctctcagg atttcactta 3660
ttttagataa tgacaacttt cttgatgcta gacaattaca tcaaggtgta ttatatctag 3720
gatgcacaca gatgtgagat tgtttcagga ttttaggtta cttggtaggt gttactataa 3780
atatctaatg agctctaatg tgtatgttca tgaaactgtg ttgcaggtac tttatcataa 3840
ttgatgatat atgggagtta ggaacatggg aaactttgaa atgtgcattt gtcaagaata 3900
cattgggcag cagaataatt atcacaacac gtatagttga tgttgccaaa tcttgttctc 3960
cttctagtga agatcttgtc tatgagatga aaccactcag tgaggctgac tcgaagaaat 4020
tgtttttcaa gaggatattt ggctgtgaag aaagctgtcc tgatagcttg aaagaagctg 4080
ccaatgatat tttgaaaaaa tgccgtggcc tacctctggc catcaatgcc atttctagcg 4140
tgttggtcac aacaagggaa acaaaagaag agtgggatcg ggtgcgacat tcaatacgct 4200
cttcaaaggt caaaagtgat ataatagaga ccatgaatta catattgtct cttagttatt 4260
ttgatcttcc ccaccaccta agaagctgct tattgtacct ggctctgttt cctgaagacc 4320
aattgattgg aaggaagcgg ttagtacgca gatggatttc tgaaggcttt atccatggag 4380
aaagtggaca agatctcatg gaattaggag aggaatattt tcaccagctt gtgaacagaa 4440
gtctaataca gcccggcaac atagggtatg acggcaaggc aatgtattgc cgagtccatg 4500
acaccatcct tgatttccta attgacaaat cctctgaaga gaacatgtgt actgtcctaa 4560
agaaacaatg caagcctaat ggcatagttc gtcggctctc tctaatgggg aatgaggatg 4620
aagaaatcgt ggagcaattg gatctgtcac acgctcggtc aatcactgct tttggggata 4680
tcaagctatt gccttcgctt gggaggtcaa aatgcttgcg cgtgctagac ttgcaagact 4740
gcgatcagtt ggaaaatcat catatcaagg atattgaaag actctatcag ctaagatact 4800
tggatatctc ttctacagga atcacggagc ttcccaggca aattggggag ttgctgtatc 4860
tagagacgct agttgcctat ggattacgtg agcttcctga aagcacaagt cggctacaac 4920
gactggcacg tttgtttgtt tactctggtt gtaagcttcc aggtgggttg ggaaacttga 4980
taaacttgca agagcttgac tgcgttgatg ccttgcattt gaagcatgtg gaagagctcg 5040
gcaaactaac aaatctgaga aagctgagta ttaaattgga cactggtggg attgaaggca 5100
acaaattaga agagtccaag gaaaagctgg tgtcctctct ctgtaaactg gacgaatgcg 5160
gcctcctttc cctgagtatt gattattatc tgagagaaaa ggatggagaa gagccgttcc 5220
tccctgcttt gggttgtatc caggaggttt ttgtatatgg acaagatatc agccggatta 5280
gcagatggct cgcgtcactt cccaacctac acaggttgct tttggacgat ccgaagatag 5340
agcagcagga tattgagatg attggattga tacccaatct gatcgatttg acactgccgc 5400
ctctatacaa aacagacgac gctgggcggc tcatcatcag gcgtgaagga tttcaacagt 5460
tgcagaagtt tgaggcttac aatactcgta tgggggtttt aatgtttgaa ccgggggcta 5520
tgccgaggct caaggagctt aaactccata acttcatcga aaagcccaaa tctgctgcgg 5580
tcgatttcga ctttggcatc caacgcctct ccagccttgc tcgcctcact gtcagcttat 5640
cctgcggtgg ctggacggtg gcagaagtag aggctgcgga ggatgctttc aagagcatgg 5700
ccgaggcgaa ccccaaccgt ccaatactgg agatgacaag atataatacg cagcatatgt 5760
tacaagacga gcaaataggt atgacaggct caggtactat taactagttt gtacttttgt 5820
cttggtgagc agtagatcaa aagatgtgca tatgcttcaa tcttttttcc ggtaatctat 5880
ttcagctaca acgccggccg tcaagtccta g 5911
<210> 26
<211> 939
<212> PRT
<213> 野生一粒小麦
<400> 26
Met Ala Glu Val Leu Val Ser Ala Ser Thr Gly Ala Met Gly Ser Leu
1 5 10 15
Leu Arg Lys Leu Gly Ala Met Leu Thr Asp Glu Tyr Lys Leu Leu Lys
20 25 30
Asn Val Arg Gly Asp Ile Lys Phe Leu Lys Asp Glu Leu Glu Val Met
35 40 45
Cys Ala Phe Leu Leu Lys Met Ser Asp Val Glu Glu Pro Asp Glu Pro
50 55 60
Thr Lys Leu Arg Val Thr Ala Val Arg Glu Met Ser Tyr Lys Ile Glu
65 70 75 80
Asp Asn Ile Asp Lys Phe Met Val Leu Val Glu His Glu Ser Ser Cys
85 90 95
Ser Glu Ala Ala His Gly Val Ala Lys Leu Met Asp Lys Cys Lys Asn
100 105 110
Leu Leu Pro Asp Ile Lys Thr Arg Arg Arg Ile Ala Lys Glu Val Lys
115 120 125
Asp Ile Lys Lys Glu Ile Lys Asp Val Ser Asp Arg Phe Ser Arg Tyr
130 135 140
Lys Ile Asp Glu Ser Ser Ser Ser Met Pro Ala Lys Gly Lys Val Asp
145 150 155 160
Pro Arg Leu Arg Ala Val Tyr Lys Asp Ala Ala Glu Leu Val Gly Ile
165 170 175
Asp Gly Pro Lys Asp Glu Leu Val Lys Trp Leu Asn Glu Lys Glu Gly
180 185 190
Gln Ser Leu Lys Ser Val Ser Ile Val Gly Tyr Gly Gly Leu Gly Lys
195 200 205
Thr Thr Leu Ala Asn Gln Ile Arg Val Asn Leu Gly Ala Thr Phe Asp
210 215 220
Cys Gly Ala Phe Val Ser Ile Ser Arg Lys Pro Asp Met Lys Ala Ile
225 230 235 240
Leu Arg Ser Ile Leu Ser Gln Ile Thr Lys Lys Asp Glu Ala Cys Ser
245 250 255
Arg Leu Asp Asp Ile Gln Leu Ile Ile Asp Lys Ile Arg Glu Phe Leu
260 265 270
Gln Asp Thr Arg Tyr Phe Ile Ile Ile Asp Asp Ile Trp Glu Leu Gly
275 280 285
Thr Trp Glu Thr Leu Lys Cys Ala Phe Val Lys Asn Thr Leu Gly Ser
290 295 300
Arg Ile Ile Ile Thr Thr Arg Ile Val Asp Val Ala Lys Ser Cys Ser
305 310 315 320
Pro Ser Ser Glu Asp Leu Val Tyr Glu Met Lys Pro Leu Ser Glu Ala
325 330 335
Asp Ser Lys Lys Leu Phe Phe Lys Arg Ile Phe Gly Cys Glu Glu Ser
340 345 350
Cys Pro Asp Ser Leu Lys Glu Ala Ala Asn Asp Ile Leu Lys Lys Cys
355 360 365
Arg Gly Leu Pro Leu Ala Ile Asn Ala Ile Ser Ser Val Leu Val Thr
370 375 380
Thr Arg Glu Thr Lys Glu Glu Trp Asp Arg Val Arg His Ser Ile Arg
385 390 395 400
Ser Ser Lys Val Lys Ser Asp Ile Ile Glu Thr Met Asn Tyr Ile Leu
405 410 415
Ser Leu Ser Tyr Phe Asp Leu Pro His His Leu Arg Ser Cys Leu Leu
420 425 430
Tyr Leu Ala Leu Phe Pro Glu Asp Gln Leu Ile Gly Arg Lys Arg Leu
435 440 445
Val Arg Arg Trp Ile Ser Glu Gly Phe Ile His Gly Glu Ser Gly Gln
450 455 460
Asp Leu Met Glu Leu Gly Glu Glu Tyr Phe His Gln Leu Val Asn Arg
465 470 475 480
Ser Leu Ile Gln Pro Gly Asn Ile Gly Tyr Asp Gly Lys Ala Met Tyr
485 490 495
Cys Arg Val His Asp Thr Ile Leu Asp Phe Leu Ile Asp Lys Ser Ser
500 505 510
Glu Glu Asn Met Cys Thr Val Leu Lys Lys Gln Cys Lys Pro Asn Gly
515 520 525
Ile Val Arg Arg Leu Ser Leu Met Gly Asn Glu Asp Glu Glu Ile Val
530 535 540
Glu Gln Leu Asp Leu Ser His Ala Arg Ser Ile Thr Ala Phe Gly Asp
545 550 555 560
Ile Lys Leu Leu Pro Ser Leu Gly Arg Ser Lys Cys Leu Arg Val Leu
565 570 575
Asp Leu Gln Asp Cys Asp Gln Leu Glu Asn His His Ile Lys Asp Ile
580 585 590
Glu Arg Leu Tyr Gln Leu Arg Tyr Leu Asp Ile Ser Ser Thr Gly Ile
595 600 605
Thr Glu Leu Pro Arg Gln Ile Gly Glu Leu Leu Tyr Leu Glu Thr Leu
610 615 620
Val Ala Tyr Gly Leu Arg Glu Leu Pro Glu Ser Thr Ser Arg Leu Gln
625 630 635 640
Arg Leu Ala Arg Leu Phe Val Tyr Ser Gly Cys Lys Leu Pro Gly Gly
645 650 655
Leu Gly Asn Leu Ile Asn Leu Gln Glu Leu Asp Cys Val Asp Ala Leu
660 665 670
His Leu Lys His Val Glu Glu Leu Gly Lys Leu Thr Asn Leu Arg Lys
675 680 685
Leu Ser Ile Lys Leu Asp Thr Gly Gly Ile Glu Gly Asn Lys Leu Glu
690 695 700
Glu Ser Lys Glu Lys Leu Val Ser Ser Leu Cys Lys Leu Asp Glu Cys
705 710 715 720
Gly Leu Leu Ser Leu Ser Ile Asp Tyr Tyr Leu Arg Glu Lys Asp Gly
725 730 735
Glu Glu Pro Phe Leu Pro Ala Leu Gly Cys Ile Gln Glu Val Phe Val
740 745 750
Tyr Gly Gln Asp Ile Ser Arg Ile Ser Arg Trp Leu Ala Ser Leu Pro
755 760 765
Asn Leu His Arg Leu Leu Leu Asp Asp Pro Lys Ile Glu Gln Gln Asp
770 775 780
Ile Glu Met Ile Gly Leu Ile Pro Asn Leu Ile Asp Leu Thr Leu Pro
785 790 795 800
Pro Leu Tyr Lys Thr Asp Asp Ala Gly Arg Leu Ile Ile Arg Arg Glu
805 810 815
Gly Phe Gln Gln Leu Gln Lys Phe Glu Ala Tyr Asn Thr Arg Met Gly
820 825 830
Val Leu Met Phe Glu Pro Gly Ala Met Pro Arg Leu Lys Glu Leu Lys
835 840 845
Leu His Asn Phe Ile Glu Lys Pro Lys Ser Ala Ala Val Asp Phe Asp
850 855 860
Phe Gly Ile Gln Arg Leu Ser Ser Leu Ala Arg Leu Thr Val Ser Leu
865 870 875 880
Ser Cys Gly Gly Trp Thr Val Ala Glu Val Glu Ala Ala Glu Asp Ala
885 890 895
Phe Lys Ser Met Ala Glu Ala Asn Pro Asn Arg Pro Ile Leu Glu Met
900 905 910
Thr Arg Tyr Asn Thr Gln His Met Leu Gln Asp Glu Gln Ile Gly Met
915 920 925
Thr Gly Ser Ala Thr Thr Pro Ala Val Lys Ser
930 935
<210> 27
<211> 2817
<212> DNA
<213> 野生一粒小麦
<400> 27
atggcggaag ttctggtgag cgcatccaca ggggcaatgg gttccctcct gcggaagttg 60
ggagctatgt tgacggatga atacaagctg ctcaagaacg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgcg ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacacgagt cctcctgctc cgaggcggct 300
catggcgtcg ccaagctcat ggacaagtgc aagaacttgc tgccggacat caagacccgc 360
cgcaggatcg ccaaggaggt caaagacatc aagaaagaaa ttaaggatgt cagcgacaga 420
ttttcaaggt acaagattga cgagtcctcc tcctctatgc cggcaaaagg caaggtcgac 480
cctcgacttc gtgcagtcta caaagacgcc gcagagctcg tcggaattga tggaccaaag 540
gatgaacttg tcaagtggct gaatgagaaa gagggccaat cactgaaatc tgtctctatt 600
gttggatatg gagggttggg caagaccacg ctcgccaacc agatccgggt caaccttgga 660
gcgaccttcg actgcggggc ttttgtctcg atttcacgca agcctgacat gaaggcgata 720
ttaagatcta tattatcgca aatcaccaag aaagacgagg cttgctctag attagatgat 780
atacaactca tcatcgacaa gattcgagaa ttcctccaag acacaaggta ctttatcata 840
attgatgata tatgggagtt aggaacatgg gaaactttga aatgtgcatt tgtcaagaat 900
acattgggca gcagaataat tatcacaaca cgtatagttg atgttgccaa atcttgttct 960
ccttctagtg aagatcttgt ctatgagatg aaaccactca gtgaggctga ctcgaagaaa 1020
ttgtttttca agaggatatt tggctgtgaa gaaagctgtc ctgatagctt gaaagaagct 1080
gccaatgata ttttgaaaaa atgccgtggc ctacctctgg ccatcaatgc catttctagc 1140
gtgttggtca caacaaggga aacaaaagaa gagtgggatc gggtgcgaca ttcaatacgc 1200
tcttcaaagg tcaaaagtga tataatagag accatgaatt acatattgtc tcttagttat 1260
tttgatcttc cccaccacct aagaagctgc ttattgtacc tggctctgtt tcctgaagac 1320
caattgattg gaaggaagcg gttagtacgc agatggattt ctgaaggctt tatccatgga 1380
gaaagtggac aagatctcat ggaattagga gaggaatatt ttcaccagct tgtgaacaga 1440
agtctaatac agcccggcaa catagggtat gacggcaagg caatgtattg ccgagtccat 1500
gacaccatcc ttgatttcct aattgacaaa tcctctgaag agaacatgtg tactgtccta 1560
aagaaacaat gcaagcctaa tggcatagtt cgtcggctct ctctaatggg gaatgaggat 1620
gaagaaatcg tggagcaatt ggatctgtca cacgctcggt caatcactgc ttttggggat 1680
atcaagctat tgccttcgct tgggaggtca aaatgcttgc gcgtgctaga cttgcaagac 1740
tgcgatcagt tggaaaatca tcatatcaag gatattgaaa gactctatca gctaagatac 1800
ttggatatct cttctacagg aatcacggag cttcccaggc aaattgggga gttgctgtat 1860
ctagagacgc tagttgccta tggattacgt gagcttcctg aaagcacaag tcggctacaa 1920
cgactggcac gtttgtttgt ttactctggt tgtaagcttc caggtgggtt gggaaacttg 1980
ataaacttgc aagagcttga ctgcgttgat gccttgcatt tgaagcatgt ggaagagctc 2040
ggcaaactaa caaatctgag aaagctgagt attaaattgg acactggtgg gattgaaggc 2100
aacaaattag aagagtccaa ggaaaagctg gtgtcctctc tctgtaaact ggacgaatgc 2160
ggcctccttt ccctgagtat tgattattat ctgagagaaa aggatggaga agagccgttc 2220
ctccctgctt tgggttgtat ccaggaggtt tttgtatatg gacaagatat cagccggatt 2280
agcagatggc tcgcgtcact tcccaaccta cacaggttgc ttttggacga tccgaagata 2340
gagcagcagg atattgagat gattggattg atacccaatc tgatcgattt gacactgccg 2400
cctctataca aaacagacga cgctgggcgg ctcatcatca ggcgtgaagg atttcaacag 2460
ttgcagaagt ttgaggctta caatactcgt atgggggttt taatgtttga accgggggct 2520
atgccgaggc tcaaggagct taaactccat aacttcatcg aaaagcccaa atctgctgcg 2580
gtcgatttcg actttggcat ccaacgcctc tccagccttg ctcgcctcac tgtcagctta 2640
tcctgcggtg gctggacggt ggcagaagta gaggctgcgg aggatgcttt caagagcatg 2700
gccgaggcga accccaaccg tccaatactg gagatgacaa gatataatac gcagcatatg 2760
ttacaagacg agcaaatagg tatgacaggc tcagctacaa cgccggccgt caagtcc 2817
<210> 28
<211> 5919
<212> DNA
<213> 野生一粒小麦
<400> 28
atggcggaag ttctggtgag cgcatccaca ggggcaatgg gttccctcct gcggaagttg 60
ggagctatgt tgacggatga atacaagctg ctcaagaacg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgcg ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacacgagt cctccttctc cgaggcggct 300
catggcgtcg ccaagctcat ggacaagtgc aagaacttgc tgccggacat caagacccgc 360
cgcaggatcg ccaaggaggt caaagacatc aagaaagaaa ttaaggatgt cagcgacaga 420
ttttcaaggt accagatctt gttctcttta cagtactatt agtacctacc atcagatttg 480
tgaccgattt gttgccactc aaggtacaag attgacgagt cctcctcctc tatgccggca 540
aaaggcaagg tcgaccctcg acttcgtgca gtctacaaag acgccgcaga gctcgtcgga 600
attgatggac caaaggatga acttgtcaag tggctgaatg agaaagaggg ccaatcactg 660
aaatctgtct ctattgttgg atatggaggg ttgggcaaga ccacgctcgc caaccagatc 720
cgggtcaacc ttggagcgac cttcgactgc ggggcttttg tctcgatttc acgcaagcct 780
gacatgaagg cgatattaag atctatatta tcacaaatca ccaagaaaga cgatgcttgc 840
tctagattag atgatataca actcatcatc gacaagattc gagaattcct ccaagacaca 900
aggtatgtat gtgagatctg ctacgaagta acattgctta attagctcat gcataatcgg 960
ttgcctagaa ttttgctaat gaagattgct ttgtgtgatt tactttcgtc ctcaacttgt 1020
atgactgcgc ttaaactttc aagtcattgt ttcttgcaac ttatgtcgat ctgttcacta 1080
attaagatat agctaaaatg tgtttgccga tttctgtcgc cacacatttt ctaatttgat 1140
tgccacaagt ttgttaaact tgcggctatg ttctgtttat taaataattg tgacatggcg 1200
tcagtcaaaa caataaagta catgcaattc cctctctttt gtacaagata aatatccgca 1260
cgcactacat gactgcaaat atatctatct tctaaggacc caaagcactt gcatgtgcag 1320
accattgtta ccggatatgc agtacattag gtttgtgatt agccacttta agaacatagg 1380
gtttctagag gctataaacc tacggttatc agtattataa tcttgaatgg atatcaaaca 1440
tccgttgaag aatctatgcc cataacacaa aaagagaaaa gggaagaatt aatcatagtc 1500
agatcggacc agcctgacta gcgtaacata atgacaacag taggttgttg cgtctttcct 1560
ggtttcgtag caatttgcat gacgaattga ggcgcagagc caccactcaa tatgtggtcc 1620
taccaatcat gaggaaataa cttgttacca aaatgacaaa agaaaatagg tagtccccat 1680
cattgtttca atatctatcc tcttgcgaac atgctattgg cagtggcaaa acattgtacc 1740
ttgccactgg caacagctgc ctttactcgg cctgagcatt catatttttt tcctcttggt 1800
tgttaaatct tgctattttt cgcttgcctt ctatggcaat cttttttttt tcttatattg 1860
ccaccaaatt gtatctaaga gcaactccaa cacgccgacg caatttttgt ccatttagat 1920
cggccaagcg gacggggtgt ctggttttgc ctttgggtgg tgcgcctacg cattagtccg 1980
cgtgggcatt taaaaaactt aaaaagctta aaataattaa atataaataa aaatgcatat 2040
taaagcccat caaagtccac ataattaaac ataatacatt aatatattaa taaaaaacag 2100
ccgccccagc ccgcagccct aggcgtcttc gtcggaggag tgggtgaggt cgaagaatgc 2160
cggcagctca gcccacggga aggcctgctg gtgggcagcc agcaccgcct gctccgctgt 2220
ctgcggcggc ggcggcccta cctggcaccg cgcacgcctc ctcctcgcgc tcctgccgct 2280
cctcctcctc atcgcacgcc caacgaagtt ttggcgctcc tccccctcgg gttcccgctg 2340
catgcggacc tggccgtcgt accgctcctc ctccagcgtg cggtccttcc acgccacgag 2400
gtaggccgcc tcctcctcca tcacgcacag ccaaatgggc ggcttgctca tccgcttgca 2460
gacgatgccc tgccattggt aaacgccggt gggcgcgggt ggtgctagcg ctggtgcagg 2520
cggtgacgat gtggctggtg gtcccggcag gtagacacta tagttggcgg ccgatagctg 2580
cagcgtgtca tcgaggccag accactagga gtcctcgcac taagactcct cggtgacgtg 2640
atgggccccc tcctccaatg ccttgaggaa cgccgcctcc tcctcaacct actatttgac 2700
caccggtggc ggcggtggag ggcaggcttc gagctggacg gcgtcgcgga ggcgcacatg 2760
gtgctcgatg gtgaaccaga cttctcagtt aggggagtct ggcctcgatg ggtggagtcg 2820
cgacgctgct ctggcgtcag gagagcacgc cgggcccgaa tctcgttggc gcgcgcacgc 2880
tcggactgtg gcaccgccgg cactggaatg tggtgggggt cgaggtgcca gccgtggggg 2940
aagttcacgt ccggccatgg cagtgagacg cggtactccc ggtggtacta cgcgtggtgc 3000
accgggatgt actggtggtc ccgtggcgct gccgccctcc catcccgcag cgccgacacc 3060
aatgcgaacc ccttcgctgc cggcctcacc ggcccttgca acgagccggc ctcgtggtcg 3120
tgcttgcctt gcccttacct ttgccaaaga aacccatgac ggctagggtt ttctgtcacc 3180
ggcgagggag cagatgtgga ctagagtggg aggagaagtg gatgaggccc gccccgccgc 3240
acccttgcaa ttaaaaaggg cgtgatccgg acgtttctag tcactgccat gcgggcccga 3300
ggtaggtggg cggcattgac tacgtctgtg gtggtagttg gctggcagac acgccagccc 3360
gaggcgcaca ccgagcggac gcgcaacacg tccgtctcgt gttcgtgtga acgcaaatca 3420
ggcacaaatt tgggctggaa atgcgtctac atggatgccg aacggacgtg atttgggttt 3480
gtgtccgcac gttgggccgg cgtttttgtg cctttcgacc ctaatggaca cacacggacc 3540
aaatccgttg gcgtgttaga gttgcttgta cctagtacta ctatgtgtgg agttgttatc 3600
tttgagtttc tcccccatct gtagctgtgt attattgtga gatgctctca ggatttcact 3660
tattttagat aatgacaact ttcttgatgc tagacaatta catcaaggtg tattatatct 3720
aggatgcaca cagatgtgag attgtttcag gattttaggt tacttggtag gtgttactat 3780
aaatatctaa tgagctctaa tgtgtatgtt catgaaactg tgttgcaggt actttatcat 3840
aattgatgat atatgggagt taggaacatg ggaaactttg aaatgtgcat ttgtcaagaa 3900
tacattgggc agcagaataa ttatcacaac acgtatagtt gatgttgcca aatcttgttc 3960
tccttctagt gaagatcttg tctatgagat gaaaccactc agtgaggctg actcgaagaa 4020
attgtttttc aagaggatat ttggctgtga agaaagctgt cctgatagct tgaaagaagc 4080
tgccaatgat attttgaaaa aatgccgtgg cctacctctg gccatcaatg ccatttctag 4140
cttgttggtc acaacaaggg aaacaaaaga agagtgggat cgggtgcgac attcaatacg 4200
ctcttcaaag gtcaaaagtg atataataga gaccatgaat tacatattgt ctcttagtta 4260
ttttgatctt ccccaccacc taagaagctg cttattgtac ctggctctgt ttcctgaaga 4320
ccaattgatt ggaaggaagc ggttagtacg cagatggatt tctgaaggct ttatccatgg 4380
agaaagtgga caagatctca tggaattagg agaggaatat tttcaccagc ttgtgaacag 4440
aagtctaata cagcccggca acatagggta tgacggcaag gcaaagtatt gccgagtcca 4500
tgacaccatc cttgatttcc taattgacaa atcctctgaa gagaacatgt gtactgtcct 4560
aaagaaacaa tgcaagccta atggcatagt tcgtcggctc tctctaatgg ggaatgagga 4620
tgaagaaatc atggagcaat tggatctgtc acacgctcgg tcaatcagtg cttttgggga 4680
tatcaagcta ttgccttcgc ttgggaggtc aaaatgcttg cgcgtgctag acttgcaagg 4740
ctgctatcag ttgaaaaatc atcatatcaa ggatattgaa agactctatc agctgagata 4800
cttggatatc tcttttacag gaatcacgga gcttcccagg caaattgggg agttgctgta 4860
tctagagacg ctagttagta catcctatgg attacgtgag cttcctgaaa gcacaagtcg 4920
gctacaacga ctggcacgtt tgtttgttga cagcggttgt aagcttccag atgggttggg 4980
aaacttgata aacttgcaag agcttgactg cgttgatgcc ttgcagttga agcatgtgga 5040
agagctcggc aaactaacaa atctgagaaa gctgaggatt aaattggaca ctgatgggat 5100
tgaaggcaac aaattagaac agtccaagga aaagctggtg tcctctctct gtaaactgga 5160
cgaatgcggc ctcctttccc tgagtattta ttattatctg agagaaaagg atggagaaga 5220
gccgttcctc cctgctttgg gttgtatcca ggaggttttt gtacatggac aagatatcag 5280
ccggattagc agatggctcg cgtcacttcc caacctacac aggttgtttt tgaaccaacc 5340
gaagatagag cagcaggata ttgagatgat tgcattgaga gagcccaatc tgatggattt 5400
gacactgtat ttatgcataa cagacgacgc tgggcggctc atcatcaagc gtgaaggatt 5460
tcaacagttg cagaggtttg agctttcccg tattcgtatg ggggatttaa tgtttgaacc 5520
gggggctatg ccgaggctca aggagcttat actctacgac ttcatcggaa agcccaaatc 5580
tggtgcggtc gatttcgact ttggcatcca acacctctcc agccttgctc gcctcactgt 5640
cggcttatcc tgcgttggct cgacggtggc ggaagtagag gccgcggagg atgctttcaa 5700
gagcatggcc gaggcgaacc ccaaccgtcc aatactggag atgacaagat ataatacgca 5760
gcatatgtta caagacgagc aaataggtat gacaggctca ggtactatta actagtttgt 5820
acttttgtct tggtgagcgg tagatcaaaa gatgtgcata tgcttcaatc ttttttccgg 5880
taatctattt cagctacaac gccggccgtc aagtcctag 5919
<210> 29
<211> 941
<212> PRT
<213> 野生一粒小麦
<400> 29
Met Ala Glu Val Leu Val Ser Ala Ser Thr Gly Ala Met Gly Ser Leu
1 5 10 15
Leu Arg Lys Leu Gly Ala Met Leu Thr Asp Glu Tyr Lys Leu Leu Lys
20 25 30
Asn Val Arg Gly Asp Ile Lys Phe Leu Lys Asp Glu Leu Glu Val Met
35 40 45
Cys Ala Phe Leu Leu Lys Met Ser Asp Val Glu Glu Pro Asp Glu Pro
50 55 60
Thr Lys Leu Arg Val Thr Ala Val Arg Glu Met Ser Tyr Lys Ile Glu
65 70 75 80
Asp Asn Ile Asp Lys Phe Met Val Leu Val Glu His Glu Ser Ser Phe
85 90 95
Ser Glu Ala Ala His Gly Val Ala Lys Leu Met Asp Lys Cys Lys Asn
100 105 110
Leu Leu Pro Asp Ile Lys Thr Arg Arg Arg Ile Ala Lys Glu Val Lys
115 120 125
Asp Ile Lys Lys Glu Ile Lys Asp Val Ser Asp Arg Phe Ser Arg Tyr
130 135 140
Lys Ile Asp Glu Ser Ser Ser Ser Met Pro Ala Lys Gly Lys Val Asp
145 150 155 160
Pro Arg Leu Arg Ala Val Tyr Lys Asp Ala Ala Glu Leu Val Gly Ile
165 170 175
Asp Gly Pro Lys Asp Glu Leu Val Lys Trp Leu Asn Glu Lys Glu Gly
180 185 190
Gln Ser Leu Lys Ser Val Ser Ile Val Gly Tyr Gly Gly Leu Gly Lys
195 200 205
Thr Thr Leu Ala Asn Gln Ile Arg Val Asn Leu Gly Ala Thr Phe Asp
210 215 220
Cys Gly Ala Phe Val Ser Ile Ser Arg Lys Pro Asp Met Lys Ala Ile
225 230 235 240
Leu Arg Ser Ile Leu Ser Gln Ile Thr Lys Lys Asp Asp Ala Cys Ser
245 250 255
Arg Leu Asp Asp Ile Gln Leu Ile Ile Asp Lys Ile Arg Glu Phe Leu
260 265 270
Gln Asp Thr Arg Tyr Phe Ile Ile Ile Asp Asp Ile Trp Glu Leu Gly
275 280 285
Thr Trp Glu Thr Leu Lys Cys Ala Phe Val Lys Asn Thr Leu Gly Ser
290 295 300
Arg Ile Ile Ile Thr Thr Arg Ile Val Asp Val Ala Lys Ser Cys Ser
305 310 315 320
Pro Ser Ser Glu Asp Leu Val Tyr Glu Met Lys Pro Leu Ser Glu Ala
325 330 335
Asp Ser Lys Lys Leu Phe Phe Lys Arg Ile Phe Gly Cys Glu Glu Ser
340 345 350
Cys Pro Asp Ser Leu Lys Glu Ala Ala Asn Asp Ile Leu Lys Lys Cys
355 360 365
Arg Gly Leu Pro Leu Ala Ile Asn Ala Ile Ser Ser Leu Leu Val Thr
370 375 380
Thr Arg Glu Thr Lys Glu Glu Trp Asp Arg Val Arg His Ser Ile Arg
385 390 395 400
Ser Ser Lys Val Lys Ser Asp Ile Ile Glu Thr Met Asn Tyr Ile Leu
405 410 415
Ser Leu Ser Tyr Phe Asp Leu Pro His His Leu Arg Ser Cys Leu Leu
420 425 430
Tyr Leu Ala Leu Phe Pro Glu Asp Gln Leu Ile Gly Arg Lys Arg Leu
435 440 445
Val Arg Arg Trp Ile Ser Glu Gly Phe Ile His Gly Glu Ser Gly Gln
450 455 460
Asp Leu Met Glu Leu Gly Glu Glu Tyr Phe His Gln Leu Val Asn Arg
465 470 475 480
Ser Leu Ile Gln Pro Gly Asn Ile Gly Tyr Asp Gly Lys Ala Lys Tyr
485 490 495
Cys Arg Val His Asp Thr Ile Leu Asp Phe Leu Ile Asp Lys Ser Ser
500 505 510
Glu Glu Asn Met Cys Thr Val Leu Lys Lys Gln Cys Lys Pro Asn Gly
515 520 525
Ile Val Arg Arg Leu Ser Leu Met Gly Asn Glu Asp Glu Glu Ile Met
530 535 540
Glu Gln Leu Asp Leu Ser His Ala Arg Ser Ile Ser Ala Phe Gly Asp
545 550 555 560
Ile Lys Leu Leu Pro Ser Leu Gly Arg Ser Lys Cys Leu Arg Val Leu
565 570 575
Asp Leu Gln Gly Cys Tyr Gln Leu Lys Asn His His Ile Lys Asp Ile
580 585 590
Glu Arg Leu Tyr Gln Leu Arg Tyr Leu Asp Ile Ser Phe Thr Gly Ile
595 600 605
Thr Glu Leu Pro Arg Gln Ile Gly Glu Leu Leu Tyr Leu Glu Thr Leu
610 615 620
Val Ser Thr Ser Tyr Gly Leu Arg Glu Leu Pro Glu Ser Thr Ser Arg
625 630 635 640
Leu Gln Arg Leu Ala Arg Leu Phe Val Asp Ser Gly Cys Lys Leu Pro
645 650 655
Asp Gly Leu Gly Asn Leu Ile Asn Leu Gln Glu Leu Asp Cys Val Asp
660 665 670
Ala Leu Gln Leu Lys His Val Glu Glu Leu Gly Lys Leu Thr Asn Leu
675 680 685
Arg Lys Leu Arg Ile Lys Leu Asp Thr Asp Gly Ile Glu Gly Asn Lys
690 695 700
Leu Glu Gln Ser Lys Glu Lys Leu Val Ser Ser Leu Cys Lys Leu Asp
705 710 715 720
Glu Cys Gly Leu Leu Ser Leu Ser Ile Tyr Tyr Tyr Leu Arg Glu Lys
725 730 735
Asp Gly Glu Glu Pro Phe Leu Pro Ala Leu Gly Cys Ile Gln Glu Val
740 745 750
Phe Val His Gly Gln Asp Ile Ser Arg Ile Ser Arg Trp Leu Ala Ser
755 760 765
Leu Pro Asn Leu His Arg Leu Phe Leu Asn Gln Pro Lys Ile Glu Gln
770 775 780
Gln Asp Ile Glu Met Ile Ala Leu Arg Glu Pro Asn Leu Met Asp Leu
785 790 795 800
Thr Leu Tyr Leu Cys Ile Thr Asp Asp Ala Gly Arg Leu Ile Ile Lys
805 810 815
Arg Glu Gly Phe Gln Gln Leu Gln Arg Phe Glu Leu Ser Arg Ile Arg
820 825 830
Met Gly Asp Leu Met Phe Glu Pro Gly Ala Met Pro Arg Leu Lys Glu
835 840 845
Leu Ile Leu Tyr Asp Phe Ile Gly Lys Pro Lys Ser Gly Ala Val Asp
850 855 860
Phe Asp Phe Gly Ile Gln His Leu Ser Ser Leu Ala Arg Leu Thr Val
865 870 875 880
Gly Leu Ser Cys Val Gly Ser Thr Val Ala Glu Val Glu Ala Ala Glu
885 890 895
Asp Ala Phe Lys Ser Met Ala Glu Ala Asn Pro Asn Arg Pro Ile Leu
900 905 910
Glu Met Thr Arg Tyr Asn Thr Gln His Met Leu Gln Asp Glu Gln Ile
915 920 925
Gly Met Thr Gly Ser Ala Thr Thr Pro Ala Val Lys Ser
930 935 940
<210> 30
<211> 2823
<212> DNA
<213> 野生一粒小麦
<400> 30
atggcggaag ttctggtgag cgcatccaca ggggcaatgg gttccctcct gcggaagttg 60
ggagctatgt tgacggatga atacaagctg ctcaagaacg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgcg ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacacgagt cctccttctc cgaggcggct 300
catggcgtcg ccaagctcat ggacaagtgc aagaacttgc tgccggacat caagacccgc 360
cgcaggatcg ccaaggaggt caaagacatc aagaaagaaa ttaaggatgt cagcgacaga 420
ttttcaaggt acaagattga cgagtcctcc tcctctatgc cggcaaaagg caaggtcgac 480
cctcgacttc gtgcagtcta caaagacgcc gcagagctcg tcggaattga tggaccaaag 540
gatgaacttg tcaagtggct gaatgagaaa gagggccaat cactgaaatc tgtctctatt 600
gttggatatg gagggttggg caagaccacg ctcgccaacc agatccgggt caaccttgga 660
gcgaccttcg actgcggggc ttttgtctcg atttcacgca agcctgacat gaaggcgata 720
ttaagatcta tattatcaca aatcaccaag aaagacgatg cttgctctag attagatgat 780
atacaactca tcatcgacaa gattcgagaa ttcctccaag acacaaggta ctttatcata 840
attgatgata tatgggagtt aggaacatgg gaaactttga aatgtgcatt tgtcaagaat 900
acattgggca gcagaataat tatcacaaca cgtatagttg atgttgccaa atcttgttct 960
ccttctagtg aagatcttgt ctatgagatg aaaccactca gtgaggctga ctcgaagaaa 1020
ttgtttttca agaggatatt tggctgtgaa gaaagctgtc ctgatagctt gaaagaagct 1080
gccaatgata ttttgaaaaa atgccgtggc ctacctctgg ccatcaatgc catttctagc 1140
ttgttggtca caacaaggga aacaaaagaa gagtgggatc gggtgcgaca ttcaatacgc 1200
tcttcaaagg tcaaaagtga tataatagag accatgaatt acatattgtc tcttagttat 1260
tttgatcttc cccaccacct aagaagctgc ttattgtacc tggctctgtt tcctgaagac 1320
caattgattg gaaggaagcg gttagtacgc agatggattt ctgaaggctt tatccatgga 1380
gaaagtggac aagatctcat ggaattagga gaggaatatt ttcaccagct tgtgaacaga 1440
agtctaatac agcccggcaa catagggtat gacggcaagg caaagtattg ccgagtccat 1500
gacaccatcc ttgatttcct aattgacaaa tcctctgaag agaacatgtg tactgtccta 1560
aagaaacaat gcaagcctaa tggcatagtt cgtcggctct ctctaatggg gaatgaggat 1620
gaagaaatca tggagcaatt ggatctgtca cacgctcggt caatcagtgc ttttggggat 1680
atcaagctat tgccttcgct tgggaggtca aaatgcttgc gcgtgctaga cttgcaaggc 1740
tgctatcagt tgaaaaatca tcatatcaag gatattgaaa gactctatca gctgagatac 1800
ttggatatct cttttacagg aatcacggag cttcccaggc aaattgggga gttgctgtat 1860
ctagagacgc tagttagtac atcctatgga ttacgtgagc ttcctgaaag cacaagtcgg 1920
ctacaacgac tggcacgttt gtttgttgac agcggttgta agcttccaga tgggttggga 1980
aacttgataa acttgcaaga gcttgactgc gttgatgcct tgcagttgaa gcatgtggaa 2040
gagctcggca aactaacaaa tctgagaaag ctgaggatta aattggacac tgatgggatt 2100
gaaggcaaca aattagaaca gtccaaggaa aagctggtgt cctctctctg taaactggac 2160
gaatgcggcc tcctttccct gagtatttat tattatctga gagaaaagga tggagaagag 2220
ccgttcctcc ctgctttggg ttgtatccag gaggtttttg tacatggaca agatatcagc 2280
cggattagca gatggctcgc gtcacttccc aacctacaca ggttgttttt gaaccaaccg 2340
aagatagagc agcaggatat tgagatgatt gcattgagag agcccaatct gatggatttg 2400
acactgtatt tatgcataac agacgacgct gggcggctca tcatcaagcg tgaaggattt 2460
caacagttgc agaggtttga gctttcccgt attcgtatgg gggatttaat gtttgaaccg 2520
ggggctatgc cgaggctcaa ggagcttata ctctacgact tcatcggaaa gcccaaatct 2580
ggtgcggtcg atttcgactt tggcatccaa cacctctcca gccttgctcg cctcactgtc 2640
ggcttatcct gcgttggctc gacggtggcg gaagtagagg ccgcggagga tgctttcaag 2700
agcatggccg aggcgaaccc caaccgtcca atactggaga tgacaagata taatacgcag 2760
catatgttac aagacgagca aataggtatg acaggctcag ctacaacgcc ggccgtcaag 2820
tcc 2823
<210> 31
<211> 5910
<212> DNA
<213> 栽培一粒小麦
<400> 31
atggcggaag ttctggtgag cgcatccaca ggggcaatgg gttccctcct gcggaagttg 60
ggagctatgt tgacggatga atacaagctg ctcaagaacg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgcg ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacacgagt cctcctgctc cgaggcggct 300
catggcgtcg ccaagctcat ggacaagtgc aagaacttgc tgccggacat caagacccgc 360
cgcaggatcg ccaaggaggt caaagacatc aagaaagaaa ttaaggatgt cagcgacaga 420
ttttcaaggt accagatctt gttctcttta cagtactatt agtacctacc atcagatttg 480
tgaccgattt gttgccactc aaggtacaag attgacgagt cctcctcctc tatgccggca 540
aaaggcaagg tcgaccctcg acttcgtgca gtctacaaag acgccgcaga gctcgtcgga 600
attgatggac caaaggatga acttgtcaag tggctgaatg agaaagaggg ccaatcactg 660
aaatctgtct ctattgttgg atatggaggg ttgggcaaga ccacgctcgc caaccagatc 720
cgggtcaacc ttggagcgac cttcgactgc ggggcttttg tctcgatttc acgcaagcct 780
gacatgaagg cgatattaag atctatatta tcacaaatca ccaagaaaga cgatgcttgc 840
tctagattag atgatataca actcatcatc gacaagattc gagaattcct ccaagacaca 900
aggtatgtat gtgagatctg ctacgaagta acattgctta attagctcat gcataatcgg 960
ttgcctagaa ttttgctaat gagattgctt tgtgtgattt actttcgtcc tcaacttgta 1020
tgactgcgct taaactttca agtcattgtt tcttgcaact tatgtcgatc tgttcactaa 1080
ttaagatata gctaaaatgt gtttgccgat ttctgtcgcc acacattttc taatttgatt 1140
gccacaagtt tgttaaactt gcggctatgt tctgtttatt aaataattgt gacatggcgt 1200
cagtcaaaac aataaagtac atgcaattcc ctctcttttg tacaagataa atatccgcac 1260
gcactacatg actgcaaata tatctatctt ctaaggaccc aaagcacttg catgtgcaga 1320
ccattgttac cggatatgca gtacattagg tttgtgatta gccactttaa gaacataggg 1380
tttctagagg ctataaacct acggttatca gtattataat cttgaatgga tatcaaacat 1440
ccgttgaaga atctttgccc ataacacaaa aagagaaaag ggaagaatta atcatagtca 1500
gatcggacca gcctgactag cgtaacataa tgacaacagt aggttgttgc gtctttcctg 1560
gtttcgtagc aatttgcatg acgaattgag gcgcagagcc accactcaat atgtggtcct 1620
accaatcatg aggaaataac ttgttaccaa aatgacaaaa gaaaataggt agtccccatc 1680
attgtttcaa tatctatcct cttgcgaaca tgctattggc agtggcaaaa cattgtacct 1740
tgccactggc aacagctgcc tttactcggc ctgagcattc atattttttt cctcttggtt 1800
gttaaatctt gctatttttc gcttgccttc tatggcaatc tttttttttc ttatattgcc 1860
accaaattgt atctaagagc aactccaaca cgccgacgca atttttgtcc atttagatcg 1920
gccaagcgga cggggtgtct ggttttgcct ttgggtggtg cgcctacgca ttagtccgcg 1980
tgggcattta aaaaacttaa aaagcttaaa ataattaaat ataaataaaa atgcatatta 2040
aagcccgtca aagtccacat aattaaacat aatacattaa tatattaata aaaaacagcc 2100
gccccagccc gcagccctag gcgtcttcgt cggaggagtg ggtgaggtcg aagaatgccg 2160
gcagctcagc ccacgggaag gcctgctggt gggcagccag caccgcctgc tccgctgtct 2220
gcggcggcgg cggccctacc tggcaccgcg cacgcctcct cctcgcgctc ctgccgctcc 2280
tcctcctcat cgcacgccca acgaagtttt ggcgctcctc cccctcgggt tcccgctgca 2340
tgcggacctg gccgtcgtac cgctcctcct ccagcgtgcg gtccttccac gccacgaggt 2400
aggccgcctc ctcctccatc acgcacagcc aaatgggcgg cttgctcatc cgcttgcaga 2460
cgatgccctg ccattggtaa acgccggtgg gcgcgggtgg tgctagcgct ggtgcaggcg 2520
gtgacgatgt ggctggtggt cccggcaggt agacactata gctggcggcc gatagctgca 2580
gcgtgtcatc gaggccagac cactaggagt cctcgcacta agactcctcg gtgacgtgat 2640
gggccccctc ctccaatgcc ttgaggaacg ccgcctcctc ctcaacctac tatttgacca 2700
ccggtggcgg cggtggaggg cagcttcgag ctggacggcg tcgcggaggc gcacatggtg 2760
ctcgatggtg aaccagactt ctcagttagg ggagtctggc ctcgatgggt ggagtcgcga 2820
cgctgctctg gcgtcaggag agcacgccgg gcccgaatct cgttggcgcg cgcacgctcg 2880
gactgtggca ccgccggcac tggaatgtgg tgggggtcga ggtgccagcc gtgggggaag 2940
ttcacgtccg gccacggcag tgagacgcgg tactcccggt ggtactacgc gtggtgcact 3000
gggatgtact ggtggtcccg tggcgctgcc gccctcccat cccgcagcgc cgacaccaat 3060
gcgaacccct tcgctgccgg cctcaccggc ccttgcaacg agccggcctc gtggtcgtgc 3120
ttgccttgcc cttacctttg ccaaagaaac ccatgacggc tagggttttc tgtcaccggc 3180
gagggagcag atgtggacta gagtgggagg agaagtggat gaggcccgcc ccgccgcacc 3240
cttgcaatta aaaagggcgt gatccggacg tttctagtca ctgccatgcg ggcccgaggt 3300
aggtgggcgg cattgactac gtctgtggtg gtagttggct ggcagacacg ccagcccgag 3360
gcgcacaccg agcggacgcg caacacgtcc gtctcgtgtt cgtgtgaacg caaatcacgc 3420
acaaatttgg gctggaaatg cgtctacatg gatgccgaac ggacgtgatt tgggtttgtg 3480
tccgcacgtt gggccggcgt ttttgtgcct ttcgacccta atggacacac acggaccaaa 3540
tccgttggcg tgttagagtt gcttgtacct agtactacta tgtgtggagt tgttatcttt 3600
gagtttctcc cccatctgta gctgtgtatt attgtgagat gctctcagga tttcacttat 3660
tttagataat gacaactttc ttgatgctag acaattacat caaggtgtat tatatctagg 3720
atgcacacag atgtgagatt gtttcaggat tttaggttac ttggtaggtg ttactataaa 3780
tatctaatga gctctaatgt gtatgttcat gaaactgtgt tgcaggtact ttatcataat 3840
tgatgatata tgggagttag gaacatggga aactgtgaaa tgtgcatttg tcaagaatac 3900
attgggcagc agaataatta tcacaacacg tatagttgat gttgccaaat cttgttctcc 3960
ttctagtgaa gatcttgtct atgagatgaa accactcagt gaggctgact cgaagaaatt 4020
gtttttcaag aggatatttg gctgtgaaga aagctgtcct gatagcttga aagaagctgc 4080
caatgatatt ttgaaaaaat gccgtggcct acctctggcc atcaatgcca tttctagctt 4140
gttggtcaca acaagggaaa caaaagaaga gtgggatcgg gtgcgacatt caatacgctc 4200
ttcaaaggtc aaaagtgata taatagagac catgaattac atattgtctc ttagttattt 4260
tgatcttccc caccacctaa gaagctgctt attgtacctg gctctgtttc ctgaagacca 4320
attgattgga aggaagcggt tagtacgcag atggatttct gaaggcttta tccatggaga 4380
aagtggacaa gatctcatgg aattaggaga ggaatatttt caccagcttg tgaacagaag 4440
tctaatacag cccgacaaca tagggtatga cggcaaggca aagtattgcc gagtccatga 4500
caccatcctt gatttcctaa tcgacaaatc ctccgaagag aacatgtgta ccgtcctaaa 4560
gaaacaatgc aagcctaatg gcatagttcg tcggctctct ctaatgggga atgaggatga 4620
agaaaccgtg gagcaattgg atctgtcaca cgctcggtca atcactgctt ttagggatat 4680
caagctattg ccttcgcttg ggaggtcaaa atgcttgcgc gtgctagact tgcaagcctg 4740
caatcagttg gaaaatcatc atatcaagga cattgaaaga ctctatcagc tgagatactt 4800
ggatatctct tttacaggaa tcacggagct tcccaggcaa attggggagt tgctgtatct 4860
agagacgcta gttacatcct atagattacg tgagcttcct gaaagcacaa gtcggctaca 4920
acgactggca cgtttgtttg ttgaccccgg ttgtaagctt ccagatgggt tgggaaactt 4980
gataaacttg caagagcttg attgggttga tgccttgcag ttgaagcatg tggaagagct 5040
cggcaaacta acaaatctga gaaagctgag gattaaattg gacactgatg ggatagaagg 5100
caacaaatta gaacagtcca aggaaaagct ggtgtcctct ctctgtaaac tggacgaatg 5160
cggcctccgt tccctgagta ttggttatta tctgagagaa aaggatggag aagagccgtt 5220
cctccctcct ttgggttgta tccaggaggt ttctgtatat ggacaagata tcagccggat 5280
tagcagatgg ctcgcgtcac ttcccaacct acacatgttg tttttcgact atgtgaagat 5340
agagcagcag gatattgaga tgattggatt gatacccaat ctgatcgatt tgtcactgtc 5400
tttacgcaaa acagacgacg ctgggcggct catcatcagg cgtgaaggat ttcaacattt 5460
gcatagtttt cgggtttacg atactcgtat gggagtttta atgtttgaac cgggggctat 5520
gccgaggctc aaggagctta aactccataa cttcatcgaa aagcccgaat ctggtgcggt 5580
cgatttcgac tttggcatcc aacgcctctc cagccttgct cgcctcactg tcagcttatt 5640
ctgcgttggc tcgacggcgg cagaagtaga ggctacggag gatgctttca agagcatggc 5700
cgaggcgaac cccaaccgtc caatactgga gatgataaga gttaatccgc accgcatgtt 5760
ctgagacgag caaatagata tggcaggctc aggtactatt aacaggtttg tactttttgt 5820
cttggtgagc agtagatcaa aagatgtgca tatgcttcat tctttttccg gtcatctatt 5880
tcagctacga cgccggccgt caagtcctag 5910
<210> 32
<211> 938
<212> PRT
<213> 栽培一粒小麦
<400> 32
Met Ala Glu Val Leu Val Ser Ala Ser Thr Gly Ala Met Gly Ser Leu
1 5 10 15
Leu Arg Lys Leu Gly Ala Met Leu Thr Asp Glu Tyr Lys Leu Leu Lys
20 25 30
Asn Val Arg Gly Asp Ile Lys Phe Leu Lys Asp Glu Leu Glu Val Met
35 40 45
Cys Ala Phe Leu Leu Lys Met Ser Asp Val Glu Glu Pro Asp Glu Pro
50 55 60
Thr Lys Leu Arg Val Thr Ala Val Arg Glu Met Ser Tyr Lys Ile Glu
65 70 75 80
Asp Asn Ile Asp Lys Phe Met Val Leu Val Glu His Glu Ser Ser Cys
85 90 95
Ser Glu Ala Ala His Gly Val Ala Lys Leu Met Asp Lys Cys Lys Asn
100 105 110
Leu Leu Pro Asp Ile Lys Thr Arg Arg Arg Ile Ala Lys Glu Val Lys
115 120 125
Asp Ile Lys Lys Glu Ile Lys Asp Val Ser Asp Arg Phe Ser Arg Tyr
130 135 140
Lys Ile Asp Glu Ser Ser Ser Ser Met Pro Ala Lys Gly Lys Val Asp
145 150 155 160
Pro Arg Leu Arg Ala Val Tyr Lys Asp Ala Ala Glu Leu Val Gly Ile
165 170 175
Asp Gly Pro Lys Asp Glu Leu Val Lys Trp Leu Asn Glu Lys Glu Gly
180 185 190
Gln Ser Leu Lys Ser Val Ser Ile Val Gly Tyr Gly Gly Leu Gly Lys
195 200 205
Thr Thr Leu Ala Asn Gln Ile Arg Val Asn Leu Gly Ala Thr Phe Asp
210 215 220
Cys Gly Ala Phe Val Ser Ile Ser Arg Lys Pro Asp Met Lys Ala Ile
225 230 235 240
Leu Arg Ser Ile Leu Ser Gln Ile Thr Lys Lys Asp Asp Ala Cys Ser
245 250 255
Arg Leu Asp Asp Ile Gln Leu Ile Ile Asp Lys Ile Arg Glu Phe Leu
260 265 270
Gln Asp Thr Arg Tyr Phe Ile Ile Ile Asp Asp Ile Trp Glu Leu Gly
275 280 285
Thr Trp Glu Thr Val Lys Cys Ala Phe Val Lys Asn Thr Leu Gly Ser
290 295 300
Arg Ile Ile Ile Thr Thr Arg Ile Val Asp Val Ala Lys Ser Cys Ser
305 310 315 320
Pro Ser Ser Glu Asp Leu Val Tyr Glu Met Lys Pro Leu Ser Glu Ala
325 330 335
Asp Ser Lys Lys Leu Phe Phe Lys Arg Ile Phe Gly Cys Glu Glu Ser
340 345 350
Cys Pro Asp Ser Leu Lys Glu Ala Ala Asn Asp Ile Leu Lys Lys Cys
355 360 365
Arg Gly Leu Pro Leu Ala Ile Asn Ala Ile Ser Ser Leu Leu Val Thr
370 375 380
Thr Arg Glu Thr Lys Glu Glu Trp Asp Arg Val Arg His Ser Ile Arg
385 390 395 400
Ser Ser Lys Val Lys Ser Asp Ile Ile Glu Thr Met Asn Tyr Ile Leu
405 410 415
Ser Leu Ser Tyr Phe Asp Leu Pro His His Leu Arg Ser Cys Leu Leu
420 425 430
Tyr Leu Ala Leu Phe Pro Glu Asp Gln Leu Ile Gly Arg Lys Arg Leu
435 440 445
Val Arg Arg Trp Ile Ser Glu Gly Phe Ile His Gly Glu Ser Gly Gln
450 455 460
Asp Leu Met Glu Leu Gly Glu Glu Tyr Phe His Gln Leu Val Asn Arg
465 470 475 480
Ser Leu Ile Gln Pro Asp Asn Ile Gly Tyr Asp Gly Lys Ala Lys Tyr
485 490 495
Cys Arg Val His Asp Thr Ile Leu Asp Phe Leu Ile Asp Lys Ser Ser
500 505 510
Glu Glu Asn Met Cys Thr Val Leu Lys Lys Gln Cys Lys Pro Asn Gly
515 520 525
Ile Val Arg Arg Leu Ser Leu Met Gly Asn Glu Asp Glu Glu Thr Val
530 535 540
Glu Gln Leu Asp Leu Ser His Ala Arg Ser Ile Thr Ala Phe Arg Asp
545 550 555 560
Ile Lys Leu Leu Pro Ser Leu Gly Arg Ser Lys Cys Leu Arg Val Leu
565 570 575
Asp Leu Gln Ala Cys Asn Gln Leu Glu Asn His His Ile Lys Asp Ile
580 585 590
Glu Arg Leu Tyr Gln Leu Arg Tyr Leu Asp Ile Ser Phe Thr Gly Ile
595 600 605
Thr Glu Leu Pro Arg Gln Ile Gly Glu Leu Leu Tyr Leu Glu Thr Leu
610 615 620
Val Thr Ser Tyr Arg Leu Arg Glu Leu Pro Glu Ser Thr Ser Arg Leu
625 630 635 640
Gln Arg Leu Ala Arg Leu Phe Val Asp Pro Gly Cys Lys Leu Pro Asp
645 650 655
Gly Leu Gly Asn Leu Ile Asn Leu Gln Glu Leu Asp Trp Val Asp Ala
660 665 670
Leu Gln Leu Lys His Val Glu Glu Leu Gly Lys Leu Thr Asn Leu Arg
675 680 685
Lys Leu Arg Ile Lys Leu Asp Thr Asp Gly Ile Glu Gly Asn Lys Leu
690 695 700
Glu Gln Ser Lys Glu Lys Leu Val Ser Ser Leu Cys Lys Leu Asp Glu
705 710 715 720
Cys Gly Leu Arg Ser Leu Ser Ile Gly Tyr Tyr Leu Arg Glu Lys Asp
725 730 735
Gly Glu Glu Pro Phe Leu Pro Pro Leu Gly Cys Ile Gln Glu Val Ser
740 745 750
Val Tyr Gly Gln Asp Ile Ser Arg Ile Ser Arg Trp Leu Ala Ser Leu
755 760 765
Pro Asn Leu His Met Leu Phe Phe Asp Tyr Val Lys Ile Glu Gln Gln
770 775 780
Asp Ile Glu Met Ile Gly Leu Ile Pro Asn Leu Ile Asp Leu Ser Leu
785 790 795 800
Ser Leu Arg Lys Thr Asp Asp Ala Gly Arg Leu Ile Ile Arg Arg Glu
805 810 815
Gly Phe Gln His Leu His Ser Phe Arg Val Tyr Asp Thr Arg Met Gly
820 825 830
Val Leu Met Phe Glu Pro Gly Ala Met Pro Arg Leu Lys Glu Leu Lys
835 840 845
Leu His Asn Phe Ile Glu Lys Pro Glu Ser Gly Ala Val Asp Phe Asp
850 855 860
Phe Gly Ile Gln Arg Leu Ser Ser Leu Ala Arg Leu Thr Val Ser Leu
865 870 875 880
Phe Cys Val Gly Ser Thr Ala Ala Glu Val Glu Ala Thr Glu Asp Ala
885 890 895
Phe Lys Ser Met Ala Glu Ala Asn Pro Asn Arg Pro Ile Leu Glu Met
900 905 910
Ile Arg Val Asn Pro His Arg Met Phe Asp Glu Gln Ile Asp Met Ala
915 920 925
Gly Ser Ala Thr Thr Pro Ala Val Lys Ser
930 935
<210> 33
<211> 2817
<212> DNA
<213> 栽培一粒小麦
<400> 33
atggcggaag ttctggtgag cgcatccaca ggggcaatgg gttccctcct gcggaagttg 60
ggagctatgt tgacggatga atacaagctg ctcaagaacg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgcg ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacacgagt cctcctgctc cgaggcggct 300
catggcgtcg ccaagctcat ggacaagtgc aagaacttgc tgccggacat caagacccgc 360
cgcaggatcg ccaaggaggt caaagacatc aagaaagaaa ttaaggatgt cagcgacaga 420
ttttcaaggt acaagattga cgagtcctcc tcctctatgc cggcaaaagg caaggtcgac 480
cctcgacttc gtgcagtcta caaagacgcc gcagagctcg tcggaattga tggaccaaag 540
gatgaacttg tcaagtggct gaatgagaaa gagggccaat cactgaaatc tgtctctatt 600
gttggatatg gagggttggg caagaccacg ctcgccaacc agatccgggt caaccttgga 660
gcgaccttcg actgcggggc ttttgtctcg atttcacgca agcctgacat gaaggcgata 720
ttaagatcta tattatcaca aatcaccaag aaagacgatg cttgctctag attagatgat 780
atacaactca tcatcgacaa gattcgagaa ttcctccaag acacaaggta ctttatcata 840
attgatgata tatgggagtt aggaacatgg gaaactgtga aatgtgcatt tgtcaagaat 900
acattgggca gcagaataat tatcacaaca cgtatagttg atgttgccaa atcttgttct 960
ccttctagtg aagatcttgt ctatgagatg aaaccactca gtgaggctga ctcgaagaaa 1020
ttgtttttca agaggatatt tggctgtgaa gaaagctgtc ctgatagctt gaaagaagct 1080
gccaatgata ttttgaaaaa atgccgtggc ctacctctgg ccatcaatgc catttctagc 1140
ttgttggtca caacaaggga aacaaaagaa gagtgggatc gggtgcgaca ttcaatacgc 1200
tcttcaaagg tcaaaagtga tataatagag accatgaatt acatattgtc tcttagttat 1260
tttgatcttc cccaccacct aagaagctgc ttattgtacc tggctctgtt tcctgaagac 1320
caattgattg gaaggaagcg gttagtacgc agatggattt ctgaaggctt tatccatgga 1380
gaaagtggac aagatctcat ggaattagga gaggaatatt ttcaccagct tgtgaacaga 1440
agtctaatac agcccgacaa catagggtat gacggcaagg caaagtattg ccgagtccat 1500
gacaccatcc ttgatttcct aatcgacaaa tcctccgaag agaacatgtg taccgtccta 1560
aagaaacaat gcaagcctaa tggcatagtt cgtcggctct ctctaatggg gaatgaggat 1620
gaagaaaccg tggagcaatt ggatctgtca cacgctcggt caatcactgc ttttagggat 1680
atcaagctat tgccttcgct tgggaggtca aaatgcttgc gcgtgctaga cttgcaagcc 1740
tgcaatcagt tggaaaatca tcatatcaag gacattgaaa gactctatca gctgagatac 1800
ttggatatct cttttacagg aatcacggag cttcccaggc aaattgggga gttgctgtat 1860
ctagagacgc tagttacatc ctatagatta cgtgagcttc ctgaaagcac aagtcggcta 1920
caacgactgg cacgtttgtt tgttgacccc ggttgtaagc ttccagatgg gttgggaaac 1980
ttgataaact tgcaagagct tgattgggtt gatgccttgc agttgaagca tgtggaagag 2040
ctcggcaaac taacaaatct gagaaagctg aggattaaat tggacactga tgggatagaa 2100
ggcaacaaat tagaacagtc caaggaaaag ctggtgtcct ctctctgtaa actggacgaa 2160
tgcggcctcc gttccctgag tattggttat tatctgagag aaaaggatgg agaagagccg 2220
ttcctccctc ctttgggttg tatccaggag gtttctgtat atggacaaga tatcagccgg 2280
attagcagat ggctcgcgtc acttcccaac ctacacatgt tgtttttcga ctatgtgaag 2340
atagagcagc aggatattga gatgattgga ttgataccca atctgatcga tttgtcactg 2400
tctttacgca aaacagacga cgctgggcgg ctcatcatca ggcgtgaagg atttcaacat 2460
ttgcatagtt ttcgggttta cgatactcgt atgggagttt taatgtttga accgggggct 2520
atgccgaggc tcaaggagct taaactccat aacttcatcg aaaagcccga atctggtgcg 2580
gtcgatttcg actttggcat ccaacgcctc tccagccttg ctcgcctcac tgtcagctta 2640
ttctgcgttg gctcgacggc ggcagaagta gaggctacgg aggatgcttt caagagcatg 2700
gccgaggcga accccaaccg tccaatactg gagatgataa gagttaatcc gcaccgcatg 2760
ttctgagacg agcaaataga tatggcaggc tcagctacga cgccggccgt caagtcc 2817
<210> 34
<211> 5908
<212> DNA
<213> 普通小麦
<400> 34
atggcggaag ttctggtgag cgcatccaca ggggcaatgg gttccctcct gcggaagttg 60
ggagctatgt tgacggatga atacaagctg ctcaagaacg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgcg ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacacgagt cctcctgctc cgaggcggct 300
catggcgtcg ccaagctcat ggacaagtgc aagaacttgc tgccggacat caagacccgc 360
cgcaggatcg ccaaggaggt caaagacatc aagaaagaaa ttaaggatgt cagcgacaga 420
ttttcaaggt accagatctt gttctcttta cagtactatt agtacctacc atcagatttg 480
tgaccgattt gttgccactc aaggtacaag attgacgagt cctcctcctc tatgccggca 540
aaaggcaagg tcgaccctcg acttcgtgca gtctacaaag acgccgcaga gctcgtcgga 600
attgatggac caaaggatga acttgtcaag tggctgaatg agaaagaggg ccaatcactg 660
aaatctgtct ctattgttgg atatggaggg ttgggcaaga ccacgctcgc caaccagatc 720
cgggtcaacc ttggagcgac cttcgactgc ggggcttttg tctcgatttc acgcaagcct 780
gacatgaagg cgatattaag atctatatta tcgcaaatca ccaagaaaga cgaggcttgc 840
tctagattag atgatataca actcatcatc gacaagattc gagaattcct ccaagacaca 900
aggtatgtat gtgagatcta ctacgaagta acattgctta attagctcat gcataatccg 960
ttgcctagaa ttttgctaat gagattgctt tgtgtgattt actttcgtcc tcaacttgta 1020
tgactgcgct taaactttca agtcattgtt tcttgcaact tatgtcgatc tgttcactaa 1080
ttaagatata gctaaaatgt gtttgccaat ttctgtcgcc acacattttc taatttgatt 1140
gccacaggtt tgttaaactt gcggctatgt tctgtttatt aaataattgt gacatggcgt 1200
cagtcaaaac aataaagtac atgcaattcc ctctcttttg tacaagataa atatccgcac 1260
gcactacatg actgcaaata tatctatctt ctaaggaccc aaagcacttg catgtgcaga 1320
ccattgttac cggatatgca gtacattagg tttgtgatta gccactttaa gaacataggg 1380
tttctagagg ctataaacct acggttatca gtattataat cttgaatgga tatcaaacat 1440
ccgttgaaga atctatgccc ataacacaaa aagagaaaag ggaagaatta atcatagtca 1500
gatcggacca gcctgactag cgtaacataa tgacaacagt aggttgttgc gtctttcctg 1560
gtttcgtagc aatttgcatg acgaattgag gcgcagagcc accactcaat atgtggtcct 1620
accaatcatg aggaaataac ttgttaccaa aatgacaaaa gaaaataggt agtccccatc 1680
attgtttcaa tatctatcct cttgcgaaca tgctattggc agtggcaaaa cattgtacct 1740
tgccactggc aacagctgcc tttactcggc ctgagcattc atattttttt cctcttggtt 1800
gttaaatctt gctatttttc gcttgccttc tatggcaatc tttttttttc ttatattgcc 1860
accaaattgt atctaagagc aactccaaca cgccgacgca atttttgtcc atttagatcg 1920
gccaagcgga cggggtgtct ggttttgcct ttgggtggtg cgcctacgca ttagtccgcg 1980
tgggcattta aaaaacttaa aaagcttaaa ataattaaat ataaataaaa atgcatatta 2040
aagcccgtca aagtccacat aattaaacat aatacattaa tatattaata aaaaacagcc 2100
gccccagccc gcagccctag gcgtcttcgt cggaggagtg ggtgaggtcg aagaatgccg 2160
gcagctcagc ccacgggaag gcctgctggt gggcagccag caccgcctgc tccgctgtct 2220
gcggcggcgg cggccctacc tggcaccgcg cacgcctcct cctcgcgctc ctgccgctcc 2280
tcctcctcat cgcacgccca acgaagtttt ggcgctcctc cccctcgggt tcccgctgca 2340
tgcggacctg gccgtcgtac cgctcctcct ccagcgtgcg gtccttccac gccacgaggt 2400
aggccgcctc ctcctccatc acgcacagcc aaatgggcgg cttgctcatc cgcttgcaga 2460
cgatgccctg ccattggtaa acgccggtgg gcgcgggtgg tgctagcgct ggtgcaggcg 2520
gtgacgatgt ggctggtggt cccggcaggt agacactata gctggcggcc gatagctgca 2580
gcgtgtcatc gaggccagac cactaggagt cctcgcacta agactcctcg gtgacgtgat 2640
gggccccctc ctccaatgcc ttgaggaacg ccgcctcctc ctcaacctac tatttgacca 2700
ccggtggcgg cggtggaggg caggcttcga gctggacggc gtcgcggagg cgcacatggt 2760
gctcgatggt gaaccagact tctcagttag gggagtctgg cctcgatggg tggagtcgcg 2820
acgctgctct ggcgtcagga gagcacgccg ggcccgaatc tcgttggcgc gcgcacgctc 2880
ggactgtggc accgccggca ctggaatgtg gtgggggtcg aggtgccagc cgtgggggaa 2940
gttcacgtcc ggccacggca gtgagacgcg gtactcccgg tggtactacg cgtggtgcac 3000
cgggatgtac tggtggtccc gtggcgctgc cgccctccca tcccgcagcg ccgacaccaa 3060
tgcgaacccc ttcgctgccg gcctcaccgg cccttgcaac gagccggcct cgtggtcgtg 3120
cttgccttgc ccttaccttt gccaaagaaa cccatgacgg ctagggtttt ctgtcaccgg 3180
cgagggagca gatgtggact agagtgggag gagaagtgga tgaggcccgc cccgccgcac 3240
ccttgcaatt aaaaagggcg tgatccggac gtttctagtc actgccatgc gggcccgagg 3300
taggtgggcg ccattgacta cgtctgtggt ggtagttggc tggcagacac gccagcccga 3360
ggcgcacacc gagcggacgc gcaacacgtc cgtctcgtgt tcgtgtgaac gcaaatcagg 3420
cacaaatttg ggctggaaat gcgtctacat ggatgccgaa cggacgtgat ttgggtttgt 3480
gtccgcacgt tgggccggcg tttttgtgcc tttcgaccct aatggacaca cacggaccaa 3540
atccgttggc gtgttagagt tgcttgtacc tagtactact atgtgtggag ttgttatctt 3600
tgagtttctc ccccatctgt agctgtgtat tattgtgaga tgctctcagg atttcactta 3660
ttttagataa tgacaacttt cttgatgcta gacaattaca tcaaggtgta ttatatctag 3720
gatgcacaca gatgtgagat tgtttcagga ttttaggtta cttggtaggt gttactataa 3780
atatctaatg agctctaatg tgtatgttca tgaaactgtg ttgcaggtac tttatcataa 3840
ttgatgatat atgggagtta ggaacatggg aaactttgaa atgtgcattt gtcaagaata 3900
cattgggcag cagaataatt atcacaacac gtatagttga tgttgccaaa tcttgttctc 3960
cttctagtga agatcttgtc tatgagatga aaccactcag tgaggctgac tcgaagaaat 4020
tgtttttcaa gaggatattt ggctgtgaag aaagctgtcc tgatagcttg aaagaagctg 4080
ccaatgatat tttgaaaaaa tgccgtggcc tacctctggc catcaatgcc atttctagcg 4140
tgttggtcac aacaagggaa acaaaagaag agtgggatcg ggtgcgacat tcaatacgct 4200
cttcaaaggt caaaagtgat ataatagaga ccatgaatta catattgtct cttagttatt 4260
ttgatcttcc ccaccaccta agaagctgct tattgtacct ggctctgttt cctgaagacc 4320
aattgattgg aaggaagcgg ttagtacgca gatggatttc tgaaggcttt atccatggag 4380
aaagtggaca agatctcatg gaattaggag aggaatattt tcaccagctt gtgaacagaa 4440
gtctaataca gcccggcaac atagggtatg acggcaaggc aatgtattgc cgagtccatg 4500
acaccatcct tgatttccta attgacaaat cctctgaaga gaacatgtgt actgtcctaa 4560
agaaacaatg caagcctaat ggcatagttc gtcggctctc tctaatgggg aatgaggatg 4620
aagaaatcgt ggagcaattg gatctgtcac acgctcggtc aatcactgct tttggggata 4680
tcaagctatt gccttcgctt gggaggtcaa aatgcttgcg cgtgctagac ttgcaagact 4740
gcgatcagtt ggaaaatcat catatcaagg atattgaaag actctatcag ctaagatact 4800
tggatatctc ttctacagga atcacggagc ttcccaggca aattggggag ttgctgtatc 4860
tagagacgct agttgcctat ggattacgtg agcttcctga aagcacaagt cggctacaac 4920
gactggcacg tttgtttgtt tactctggtt gtaagcttcc aggtgggttg ggaaacttga 4980
taaacttgca agagcttgac tgcgttgatg ccttgcattt gaagcatgtg gaagagctcg 5040
gcaaactaac aaatctgaga aagctgagta ttaaattgga cactggtggg attgaaggca 5100
acaaattaga agagtccaag gaaaagctgg tgtcctctct ctgtaaactg gacgaatgcg 5160
gcctcctttc cctgagtatt gattattatc tgagagaaaa ggatggagaa gagccgttcc 5220
tccctgcttt gggttgtatc caggaggttt ttgtatatgg acaagatatc agccggatta 5280
gcagatggct cgcgtcactt cccaacctac acgggttgct tttggacaat ccgaagatag 5340
agcagcagga tattgagatg attggattga tacccaatct gatcgatttg acactgtatt 5400
tacgcataac agacgacgct gggcggctca tcatcaagcg tgaaggattt caacagttgc 5460
agaggtttga gctttcccgt attcgtatgg gggatttaat gtttgaaccg ggggctatgc 5520
cgaggctcaa ggagcttata ctctaccact tcatcgaaaa gcccaaatct ggtgcggtcg 5580
atttcgactt tggcatccaa cacctctcca gccttgctcg cctcactgtc ggcttattat 5640
gcgttggctc gacggcggcg gaagtagagg ctgcggagga tgctttcaag agcatggccg 5700
aggcgaaccc caaccgtcca atactggaga tgacaagact ttatccgcag catatgttac 5760
aagacgagca aataggtatg acaggctcag gtactattaa ctagtttgta cttttgtctt 5820
ggtgagcagt agatcaaaag atgtgcatat gcttcaatct tttttccggt aatctatttc 5880
agctacaacg ccggccgtca agtcctag 5908
<210> 35
<211> 938
<212> PRT
<213> 普通小麦
<400> 35
Met Ala Glu Val Leu Val Ser Ala Ser Thr Gly Ala Met Gly Ser Leu
1 5 10 15
Leu Arg Lys Leu Gly Ala Met Leu Thr Asp Glu Tyr Lys Leu Leu Lys
20 25 30
Asn Val Arg Gly Asp Ile Lys Phe Leu Lys Asp Glu Leu Glu Val Met
35 40 45
Cys Ala Phe Leu Leu Lys Met Ser Asp Val Glu Glu Pro Asp Glu Pro
50 55 60
Thr Lys Leu Arg Val Thr Ala Val Arg Glu Met Ser Tyr Lys Ile Glu
65 70 75 80
Asp Asn Ile Asp Lys Phe Met Val Leu Val Glu His Glu Ser Ser Cys
85 90 95
Ser Glu Ala Ala His Gly Val Ala Lys Leu Met Asp Lys Cys Lys Asn
100 105 110
Leu Leu Pro Asp Ile Lys Thr Arg Arg Arg Ile Ala Lys Glu Val Lys
115 120 125
Asp Ile Lys Lys Glu Ile Lys Asp Val Ser Asp Arg Phe Ser Arg Tyr
130 135 140
Lys Ile Asp Glu Ser Ser Ser Ser Met Pro Ala Lys Gly Lys Val Asp
145 150 155 160
Pro Arg Leu Arg Ala Val Tyr Lys Asp Ala Ala Glu Leu Val Gly Ile
165 170 175
Asp Gly Pro Lys Asp Glu Leu Val Lys Trp Leu Asn Glu Lys Glu Gly
180 185 190
Gln Ser Leu Lys Ser Val Ser Ile Val Gly Tyr Gly Gly Leu Gly Lys
195 200 205
Thr Thr Leu Ala Asn Gln Ile Arg Val Asn Leu Gly Ala Thr Phe Asp
210 215 220
Cys Gly Ala Phe Val Ser Ile Ser Arg Lys Pro Asp Met Lys Ala Ile
225 230 235 240
Leu Arg Ser Ile Leu Ser Gln Ile Thr Lys Lys Asp Glu Ala Cys Ser
245 250 255
Arg Leu Asp Asp Ile Gln Leu Ile Ile Asp Lys Ile Arg Glu Phe Leu
260 265 270
Gln Asp Thr Arg Tyr Phe Ile Ile Ile Asp Asp Ile Trp Glu Leu Gly
275 280 285
Thr Trp Glu Thr Leu Lys Cys Ala Phe Val Lys Asn Thr Leu Gly Ser
290 295 300
Arg Ile Ile Ile Thr Thr Arg Ile Val Asp Val Ala Lys Ser Cys Ser
305 310 315 320
Pro Ser Ser Glu Asp Leu Val Tyr Glu Met Lys Pro Leu Ser Glu Ala
325 330 335
Asp Ser Lys Lys Leu Phe Phe Lys Arg Ile Phe Gly Cys Glu Glu Ser
340 345 350
Cys Pro Asp Ser Leu Lys Glu Ala Ala Asn Asp Ile Leu Lys Lys Cys
355 360 365
Arg Gly Leu Pro Leu Ala Ile Asn Ala Ile Ser Ser Val Leu Val Thr
370 375 380
Thr Arg Glu Thr Lys Glu Glu Trp Asp Arg Val Arg His Ser Ile Arg
385 390 395 400
Ser Ser Lys Val Lys Ser Asp Ile Ile Glu Thr Met Asn Tyr Ile Leu
405 410 415
Ser Leu Ser Tyr Phe Asp Leu Pro His His Leu Arg Ser Cys Leu Leu
420 425 430
Tyr Leu Ala Leu Phe Pro Glu Asp Gln Leu Ile Gly Arg Lys Arg Leu
435 440 445
Val Arg Arg Trp Ile Ser Glu Gly Phe Ile His Gly Glu Ser Gly Gln
450 455 460
Asp Leu Met Glu Leu Gly Glu Glu Tyr Phe His Gln Leu Val Asn Arg
465 470 475 480
Ser Leu Ile Gln Pro Gly Asn Ile Gly Tyr Asp Gly Lys Ala Met Tyr
485 490 495
Cys Arg Val His Asp Thr Ile Leu Asp Phe Leu Ile Asp Lys Ser Ser
500 505 510
Glu Glu Asn Met Cys Thr Val Leu Lys Lys Gln Cys Lys Pro Asn Gly
515 520 525
Ile Val Arg Arg Leu Ser Leu Met Gly Asn Glu Asp Glu Glu Ile Val
530 535 540
Glu Gln Leu Asp Leu Ser His Ala Arg Ser Ile Thr Ala Phe Gly Asp
545 550 555 560
Ile Lys Leu Leu Pro Ser Leu Gly Arg Ser Lys Cys Leu Arg Val Leu
565 570 575
Asp Leu Gln Asp Cys Asp Gln Leu Glu Asn His His Ile Lys Asp Ile
580 585 590
Glu Arg Leu Tyr Gln Leu Arg Tyr Leu Asp Ile Ser Ser Thr Gly Ile
595 600 605
Thr Glu Leu Pro Arg Gln Ile Gly Glu Leu Leu Tyr Leu Glu Thr Leu
610 615 620
Val Ala Tyr Gly Leu Arg Glu Leu Pro Glu Ser Thr Ser Arg Leu Gln
625 630 635 640
Arg Leu Ala Arg Leu Phe Val Tyr Ser Gly Cys Lys Leu Pro Gly Gly
645 650 655
Leu Gly Asn Leu Ile Asn Leu Gln Glu Leu Asp Cys Val Asp Ala Leu
660 665 670
His Leu Lys His Val Glu Glu Leu Gly Lys Leu Thr Asn Leu Arg Lys
675 680 685
Leu Ser Ile Lys Leu Asp Thr Gly Gly Ile Glu Gly Asn Lys Leu Glu
690 695 700
Glu Ser Lys Glu Lys Leu Val Ser Ser Leu Cys Lys Leu Asp Glu Cys
705 710 715 720
Gly Leu Leu Ser Leu Ser Ile Asp Tyr Tyr Leu Arg Glu Lys Asp Gly
725 730 735
Glu Glu Pro Phe Leu Pro Ala Leu Gly Cys Ile Gln Glu Val Phe Val
740 745 750
Tyr Gly Gln Asp Ile Ser Arg Ile Ser Arg Trp Leu Ala Ser Leu Pro
755 760 765
Asn Leu His Gly Leu Leu Leu Asp Asn Pro Lys Ile Glu Gln Gln Asp
770 775 780
Ile Glu Met Ile Gly Leu Ile Pro Asn Leu Ile Asp Leu Thr Leu Tyr
785 790 795 800
Leu Arg Ile Thr Asp Asp Ala Gly Arg Leu Ile Ile Lys Arg Glu Gly
805 810 815
Phe Gln Gln Leu Gln Arg Phe Glu Leu Ser Arg Ile Arg Met Gly Asp
820 825 830
Leu Met Phe Glu Pro Gly Ala Met Pro Arg Leu Lys Glu Leu Ile Leu
835 840 845
Tyr His Phe Ile Glu Lys Pro Lys Ser Gly Ala Val Asp Phe Asp Phe
850 855 860
Gly Ile Gln His Leu Ser Ser Leu Ala Arg Leu Thr Val Gly Leu Leu
865 870 875 880
Cys Val Gly Ser Thr Ala Ala Glu Val Glu Ala Ala Glu Asp Ala Phe
885 890 895
Lys Ser Met Ala Glu Ala Asn Pro Asn Arg Pro Ile Leu Glu Met Thr
900 905 910
Arg Leu Tyr Pro Gln His Met Leu Gln Asp Glu Gln Ile Gly Met Thr
915 920 925
Gly Ser Ala Thr Thr Pro Ala Val Lys Ser
930 935
<210> 36
<211> 2814
<212> DNA
<213> 普通小麦
<400> 36
atggcggaag ttctggtgag cgcatccaca ggggcaatgg gttccctcct gcggaagttg 60
ggagctatgt tgacggatga atacaagctg ctcaagaacg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgcg ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacacgagt cctcctgctc cgaggcggct 300
catggcgtcg ccaagctcat ggacaagtgc aagaacttgc tgccggacat caagacccgc 360
cgcaggatcg ccaaggaggt caaagacatc aagaaagaaa ttaaggatgt cagcgacaga 420
ttttcaaggt acaagattga cgagtcctcc tcctctatgc cggcaaaagg caaggtcgac 480
cctcgacttc gtgcagtcta caaagacgcc gcagagctcg tcggaattga tggaccaaag 540
gatgaacttg tcaagtggct gaatgagaaa gagggccaat cactgaaatc tgtctctatt 600
gttggatatg gagggttggg caagaccacg ctcgccaacc agatccgggt caaccttgga 660
gcgaccttcg actgcggggc ttttgtctcg atttcacgca agcctgacat gaaggcgata 720
ttaagatcta tattatcgca aatcaccaag aaagacgagg cttgctctag attagatgat 780
atacaactca tcatcgacaa gattcgagaa ttcctccaag acacaaggta ctttatcata 840
attgatgata tatgggagtt aggaacatgg gaaactttga aatgtgcatt tgtcaagaat 900
acattgggca gcagaataat tatcacaaca cgtatagttg atgttgccaa atcttgttct 960
ccttctagtg aagatcttgt ctatgagatg aaaccactca gtgaggctga ctcgaagaaa 1020
ttgtttttca agaggatatt tggctgtgaa gaaagctgtc ctgatagctt gaaagaagct 1080
gccaatgata ttttgaaaaa atgccgtggc ctacctctgg ccatcaatgc catttctagc 1140
gtgttggtca caacaaggga aacaaaagaa gagtgggatc gggtgcgaca ttcaatacgc 1200
tcttcaaagg tcaaaagtga tataatagag accatgaatt acatattgtc tcttagttat 1260
tttgatcttc cccaccacct aagaagctgc ttattgtacc tggctctgtt tcctgaagac 1320
caattgattg gaaggaagcg gttagtacgc agatggattt ctgaaggctt tatccatgga 1380
gaaagtggac aagatctcat ggaattagga gaggaatatt ttcaccagct tgtgaacaga 1440
agtctaatac agcccggcaa catagggtat gacggcaagg caatgtattg ccgagtccat 1500
gacaccatcc ttgatttcct aattgacaaa tcctctgaag agaacatgtg tactgtccta 1560
aagaaacaat gcaagcctaa tggcatagtt cgtcggctct ctctaatggg gaatgaggat 1620
gaagaaatcg tggagcaatt ggatctgtca cacgctcggt caatcactgc ttttggggat 1680
atcaagctat tgccttcgct tgggaggtca aaatgcttgc gcgtgctaga cttgcaagac 1740
tgcgatcagt tggaaaatca tcatatcaag gatattgaaa gactctatca gctaagatac 1800
ttggatatct cttctacagg aatcacggag cttcccaggc aaattgggga gttgctgtat 1860
ctagagacgc tagttgccta tggattacgt gagcttcctg aaagcacaag tcggctacaa 1920
cgactggcac gtttgtttgt ttactctggt tgtaagcttc caggtgggtt gggaaacttg 1980
ataaacttgc aagagcttga ctgcgttgat gccttgcatt tgaagcatgt ggaagagctc 2040
ggcaaactaa caaatctgag aaagctgagt attaaattgg acactggtgg gattgaaggc 2100
aacaaattag aagagtccaa ggaaaagctg gtgtcctctc tctgtaaact ggacgaatgc 2160
ggcctccttt ccctgagtat tgattattat ctgagagaaa aggatggaga agagccgttc 2220
ctccctgctt tgggttgtat ccaggaggtt tttgtatatg gacaagatat cagccggatt 2280
agcagatggc tcgcgtcact tcccaaccta cacgggttgc ttttggacaa tccgaagata 2340
gagcagcagg atattgagat gattggattg atacccaatc tgatcgattt gacactgtat 2400
ttacgcataa cagacgacgc tgggcggctc atcatcaagc gtgaaggatt tcaacagttg 2460
cagaggtttg agctttcccg tattcgtatg ggggatttaa tgtttgaacc gggggctatg 2520
ccgaggctca aggagcttat actctaccac ttcatcgaaa agcccaaatc tggtgcggtc 2580
gatttcgact ttggcatcca acacctctcc agccttgctc gcctcactgt cggcttatta 2640
tgcgttggct cgacggcggc ggaagtagag gctgcggagg atgctttcaa gagcatggcc 2700
gaggcgaacc ccaaccgtcc aatactggag atgacaagac tttatccgca gcatatgtta 2760
caagacgagc aaataggtat gacaggctca gctacaacgc cggccgtcaa gtcc 2814
<210> 37
<211> 5995
<212> DNA
<213> 栽培一粒小麦
<400> 37
atggcggaag ttctggtgag cgcatccaca ggggcaatgg gttccctcct gcggaagttg 60
ggagctatgt tgacggatga atacaagctg ctcaagaacg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgcg ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacacgagt cctccagttc cgaggctcat 300
ggcgtcacca agctcatgga caagtgcaag aacttgctgc cggatatcaa gacccgccgc 360
aggatcgcca aggaggtcaa agacatcaag aaagaaatta aggatgtcag cgacagattt 420
ttaaggtacc agatcttgtt ctctttacag tactattagt acctaccatc agatttgcga 480
ctgagctgat gcttccatcc atctatccga tttgttgcca ctcaaggtac aagattgacg 540
actcctcctc ctctatgcct gctgcaaaag acaaggtcga ccctcgactt cgtgcagtct 600
acaaagacgc cgcagagctc gtcggaattg atggaccaaa ggatgaactt gtcaagtggc 660
tgaatgagaa agagggccaa tcacttaaat ctgtctctat tgttggatat ggagggttgg 720
gcaagaccac gctcgccaac cagatccggg tcaaccttgg agcgactttc gactgcgggg 780
cttttgtctc gatttcacgc aagcctgaca tgaaggcgat attaagatct atattatcgc 840
aaatcaccaa gaaagacgag gcttgcccta gattagatga tatacaactc atcatcgaca 900
agattcgaga attcctccaa gacacaaggt atatatgtga catctgctac aaagtagcat 960
tgcttaatta gctcatgtat aatctgttgc ctagaatctt gctaatgaag attgctttgt 1020
gtgatttaca ttcgtcctca acttgtatga ccgcgtttaa aatttgaagt cattgttttt 1080
tgcaacttat gtcgatctgt tcactataag atatagctaa aatctgtatg ccgatttctg 1140
gcgccataca ttttctaatt gattgccaca ggtttgtcaa gcttgcggct atgcatggag 1200
ccagtgaaaa caaataagta cacgcaattc ccattttttt acaagacaaa tatccgcacg 1260
cactacatga ctgcaaatat atctactccc tccgtctcat aatataggat gtttttgaca 1320
ctagtgtagt gtagtgtcaa aaaacgtctt acattacaag atggaaggag tatcttctaa 1380
gaacccaaag cacttggatg tgcagaccac tgttaccgga tatgcagtgc attaggtttg 1440
tgattaacca ctttaagaac atagggtttc tagaggctat aaacctacgg ttatcagtat 1500
tataatcctg aatgcatatc aaacattgaa gaacctatat cctaatacaa aaagagaaaa 1560
ggggggaatt aatagaggaa ttaatcatgg tcagaacgga ccagcctgac tagcgtaaca 1620
taatgataat cgtagtttgt tgcttctttc tggtttcgta gcaatttgca tgatgaattg 1680
aggcgcagag cctcctacca atcatgagga aataacttgt taccaaaaag acaaaagaaa 1740
ataggtagtc ccaatcattg tttcgattct atccttttcc gaacatgcta ttggcagtgg 1800
caaaacggaa ttgctccatg cacgaccttg tgcccgatgc atgcacgaca gaaaatccag 1860
cggctggtac tctttccctc agacgtatgt ccggctagat ttcattcatg gtgcgtgaat 1920
tgtctgattt atgtcgtgtg tatagcacga ctcgtggcaa aacattgtac cttgccactg 1980
gcaacagctt ccttgacccg gcctgagcaa ctcatcaagg aattcataat ttttcctctt 2040
ggttgttaaa tcttgctatt ttttgtttgc cttctatggc aatctttttt ttcttatatt 2100
gcaaccaaat tgtatctaag agaaactcca acgcgccgac gcagtttttg tccgttcgga 2160
ttggccaagc ggacgggggt gtccggtttt gcctttgggt gcgtaggcgc gcccaacacg 2220
gcctacacat tagtccgtgt gggcatttaa aaaaacttaa aaagcttaaa ataattgaaa 2280
aaaaatgcat attaaagccc atcaaagtcc acataattaa acataaaaca ttaaaacact 2340
aataagaaac gccgcccagc cgccccaacc tacagcccta ggcatcgtct tcgtcgttgg 2400
agtggctgag gtcgaagaat gccggcagct cggcccatgg gaaggcctgc cggtaggatg 2460
ctagcgctgc ctgctctgcc atctgctgat gtggtggctc tgcctggcgc cgcgcgcacc 2520
tcttcctcgc gttcccaatg aagtcggaac tcctcctcgg gctcccgttg catacggaac 2580
tggccgtcat ctcgctcctc ctctagcgtg tggtccttcc acgccgcgag gtaggccgcc 2640
tcctcctcct ccgtcgcgcc cagccaaatg agtagcgcac tcacccactc ccggacaaca 2700
ccctgccatt agtgaacgct ggtgggcacg ggtggtgcca gcgctggtgc gggcggtgac 2760
gatggggcca gtggtgccgg taggtagaca ctatcgcggg cggtcgacag ctgcaacgca 2820
tcctcgaggc caggccacca ggagtcctcg tgctgggact ccaggatgac caccagtggc 2880
agcggtggag ggcgggcatg gagctggatg gcgccgtggc ggcgcagatc gtgctcggtt 2940
gggaaccaga cgtcccagtt agggagtctg gcgtgtaggc ggggtcgcgg cactgcttcg 3000
gcgttaggag agcatgcagg cccaaaatcg ttggcgcgcg cacactcgga ctgtggcatg 3060
gcctgtaccg gaatgtggtg ggggtcgacg tgccaaccat gggggaggtt cacgtccggc 3120
cacggcagcg ggacgcggta ctcccagtgg tactacgcgt ggtgcaccga ggtgtaccgg 3180
tggcgctgcc gcccttccgt cctgatgcac cgacactaat gcgaacccct tcgtcggcgg 3240
cctcaccggc ccttgcaacg agtcggcctc gtggttgtgc ttgcccttgc ccttgccttt 3300
gccgaagaag ctcatgacgg ctagggtttt ttgtcgccgg cgagggagca gatgtggact 3360
agagtgggag gagaagtgga ttaggccggc cccgccgtac ccttgcaatt aaaaaaggcg 3420
cagtctgaac acttctagtc actgccatgc gggcccgagg taggtgggcg ggcattaact 3480
acgactgcgg tggtagttgg ctggccgaca cgcgggcccg aggcggacac cgagtggacg 3540
cgcagcacct ccgtctcgtg ttcgtgcgga tgcaaatcag gcgcattttt gtccgttttg 3600
acccaaacgg acacgagcgc atggaccaaa tgcgccggca cgttggagtt gcttgtacct 3660
agtactatgt gtggagttgt tatcttgcag tttcttcccc atttgtagct gtgtattatt 3720
gtgagatgct ctcagggatt tcacttatcg tacaagtatg ttttagacaa ttacatcaag 3780
gtgtattata attaggatgt acacagatgt tacattgttt caggaattta ggttacttga 3840
taggtattac tataaatatc taatgagctc taatgtgtat gttcatgaaa ctgtgttgca 3900
ggtactttat cataattgat gatatatggg agttaggaac atgggaaact ttgaaatgtg 3960
catttgtcaa gaatacattg ggcagcagaa taattatcac aacacgtata gttgatgttg 4020
ccaaatcttg ctctccttct agtgaagatc ttgtctatga gatgaaacca ctcagtgagg 4080
ctgactcgaa gaaattgttt ttcaagagga tatttggctg tgaagaaagc tgtcctgata 4140
gcttgaaaga agctgccaat gatattttga agaaatgccg tggtctacct ctggccatca 4200
atgccatttc tagcctgttg gccacaacaa gggaaacaaa agaagagtgg gatcgggtgc 4260
gacattcaat acgctcttca aaggtcaaaa gtgatataat agagaccatg aattacatat 4320
tgtctcttag ttattttgat cttccccacc acctaagaag ctgcttattg tacctggctc 4380
tgtttcctga agaccaattg attgaaagga agcggttggt acgcagatgg atttctgaag 4440
gctttatcca tggagaaagt ggacaagctc tcatggaatt aggagaggaa tattttcacc 4500
agcttgtgaa cagaagtcta atacagcccg gcaacatagg gtatgacggc aaggcggagt 4560
attgccgagt ccatgacacc atccttgatt tcctaattga caaatcctct gaagagaaca 4620
tgtgtactgt cctgaagaaa caatgcaagc ctaatggcat agttcgtcgg ctctctctaa 4680
tggggaatga ggatgaagaa atcatggagc aattggatct gtcacacgct cggtcaatca 4740
gtgcttttgg ggatatcaag ctattgcctt cgcttgggag gtcaaaatgc ttgcgcgtgc 4800
tagacttgca aggctgctat cagttgaaaa atcatcatat caaggatatt gaaagactct 4860
atcagctgag atacttggat atctctttta caggaatcac ggagcttccc aggcaaattg 4920
gggagttgct gtatctagag acgctagtta catcctatgg attacgtgag cttcctgaaa 4980
gcacaagtcg gctacaacga ctggcacgtt tgtttgttta ccacggttgt aagcttccag 5040
atgggttggg aaacttggta aacttgcaag agcttgagtg cgttgatgcc ttgcagttga 5100
agcatgtgga agagctcggc aaactaacaa atctgagaaa gctgagtatt aaattggaca 5160
ctggtgggat tgaaggcaac aaattagaag agtccaagga aaagctggtg tcctctctct 5220
gtaaactgga cgaatgcggc ctccgttccc tgagtattca ttattatctg agagaaaagg 5280
atggagaaga gccgttcctc cctgctttgg gttgtatcca ggaggtttgt gtatatggac 5340
aagatatcag ccggattagc agatggctcg cgtcacttcc caacctacac aggttgtttt 5400
tcaacgatcc caaaatgaag atagagcagc aggatattga gatgattgga ttgataccca 5460
atctgatgga tttgacactg tatttacgca taacagacga cgctatgccg aggctcatca 5520
tcaagcgtga aggatttcaa cagttgcaga ggtttgagct ttcccgtatt cgtatggggg 5580
atttaatgtt tgaaccgggg gctatgccga ggctcaagga gcttatactc cataacttca 5640
tcgaaaagcc caaatctggt gcggtcgatt tcgactttgg catccaacac ctctccagcc 5700
ttgctcgcct cactgtcggc ttattatgcg ttggctcgac ggcggcggaa gtagaggctg 5760
cggaggatgc tttcaagagc atggccgagg cgaaccccaa ccgtccaata ctggagatga 5820
caagactttg tccgcagcat atgttacaag acgagcaaat aggtatgaca ggctcaggta 5880
ctattaacta gtttgtactt ttgtcttggt gagcagtaga tcaaaagatg tgcatatgct 5940
tcaatctttt ttccggtaat ctatttcagc tacaacgccg gccgtcaagt cctag 5995
<210> 38
<211> 942
<212> PRT
<213> 栽培一粒小麦
<400> 38
Met Ala Glu Val Leu Val Ser Ala Ser Thr Gly Ala Met Gly Ser Leu
1 5 10 15
Leu Arg Lys Leu Gly Ala Met Leu Thr Asp Glu Tyr Lys Leu Leu Lys
20 25 30
Asn Val Arg Gly Asp Ile Lys Phe Leu Lys Asp Glu Leu Glu Val Met
35 40 45
Cys Ala Phe Leu Leu Lys Met Ser Asp Val Glu Glu Pro Asp Glu Pro
50 55 60
Thr Lys Leu Arg Val Thr Ala Val Arg Glu Met Ser Tyr Lys Ile Glu
65 70 75 80
Asp Asn Ile Asp Lys Phe Met Val Leu Val Glu His Glu Ser Ser Ser
85 90 95
Ser Glu Ala His Gly Val Thr Lys Leu Met Asp Lys Cys Lys Asn Leu
100 105 110
Leu Pro Asp Ile Lys Thr Arg Arg Arg Ile Ala Lys Glu Val Lys Asp
115 120 125
Ile Lys Lys Glu Ile Lys Asp Val Ser Asp Arg Phe Leu Arg Tyr Lys
130 135 140
Ile Asp Asp Ser Ser Ser Ser Met Pro Ala Ala Lys Asp Lys Val Asp
145 150 155 160
Pro Arg Leu Arg Ala Val Tyr Lys Asp Ala Ala Glu Leu Val Gly Ile
165 170 175
Asp Gly Pro Lys Asp Glu Leu Val Lys Trp Leu Asn Glu Lys Glu Gly
180 185 190
Gln Ser Leu Lys Ser Val Ser Ile Val Gly Tyr Gly Gly Leu Gly Lys
195 200 205
Thr Thr Leu Ala Asn Gln Ile Arg Val Asn Leu Gly Ala Thr Phe Asp
210 215 220
Cys Gly Ala Phe Val Ser Ile Ser Arg Lys Pro Asp Met Lys Ala Ile
225 230 235 240
Leu Arg Ser Ile Leu Ser Gln Ile Thr Lys Lys Asp Glu Ala Cys Pro
245 250 255
Arg Leu Asp Asp Ile Gln Leu Ile Ile Asp Lys Ile Arg Glu Phe Leu
260 265 270
Gln Asp Thr Arg Tyr Phe Ile Ile Ile Asp Asp Ile Trp Glu Leu Gly
275 280 285
Thr Trp Glu Thr Leu Lys Cys Ala Phe Val Lys Asn Thr Leu Gly Ser
290 295 300
Arg Ile Ile Ile Thr Thr Arg Ile Val Asp Val Ala Lys Ser Cys Ser
305 310 315 320
Pro Ser Ser Glu Asp Leu Val Tyr Glu Met Lys Pro Leu Ser Glu Ala
325 330 335
Asp Ser Lys Lys Leu Phe Phe Lys Arg Ile Phe Gly Cys Glu Glu Ser
340 345 350
Cys Pro Asp Ser Leu Lys Glu Ala Ala Asn Asp Ile Leu Lys Lys Cys
355 360 365
Arg Gly Leu Pro Leu Ala Ile Asn Ala Ile Ser Ser Leu Leu Ala Thr
370 375 380
Thr Arg Glu Thr Lys Glu Glu Trp Asp Arg Val Arg His Ser Ile Arg
385 390 395 400
Ser Ser Lys Val Lys Ser Asp Ile Ile Glu Thr Met Asn Tyr Ile Leu
405 410 415
Ser Leu Ser Tyr Phe Asp Leu Pro His His Leu Arg Ser Cys Leu Leu
420 425 430
Tyr Leu Ala Leu Phe Pro Glu Asp Gln Leu Ile Glu Arg Lys Arg Leu
435 440 445
Val Arg Arg Trp Ile Ser Glu Gly Phe Ile His Gly Glu Ser Gly Gln
450 455 460
Ala Leu Met Glu Leu Gly Glu Glu Tyr Phe His Gln Leu Val Asn Arg
465 470 475 480
Ser Leu Ile Gln Pro Gly Asn Ile Gly Tyr Asp Gly Lys Ala Glu Tyr
485 490 495
Cys Arg Val His Asp Thr Ile Leu Asp Phe Leu Ile Asp Lys Ser Ser
500 505 510
Glu Glu Asn Met Cys Thr Val Leu Lys Lys Gln Cys Lys Pro Asn Gly
515 520 525
Ile Val Arg Arg Leu Ser Leu Met Gly Asn Glu Asp Glu Glu Ile Met
530 535 540
Glu Gln Leu Asp Leu Ser His Ala Arg Ser Ile Ser Ala Phe Gly Asp
545 550 555 560
Ile Lys Leu Leu Pro Ser Leu Gly Arg Ser Lys Cys Leu Arg Val Leu
565 570 575
Asp Leu Gln Gly Cys Tyr Gln Leu Lys Asn His His Ile Lys Asp Ile
580 585 590
Glu Arg Leu Tyr Gln Leu Arg Tyr Leu Asp Ile Ser Phe Thr Gly Ile
595 600 605
Thr Glu Leu Pro Arg Gln Ile Gly Glu Leu Leu Tyr Leu Glu Thr Leu
610 615 620
Val Thr Ser Tyr Gly Leu Arg Glu Leu Pro Glu Ser Thr Ser Arg Leu
625 630 635 640
Gln Arg Leu Ala Arg Leu Phe Val Tyr His Gly Cys Lys Leu Pro Asp
645 650 655
Gly Leu Gly Asn Leu Val Asn Leu Gln Glu Leu Glu Cys Val Asp Ala
660 665 670
Leu Gln Leu Lys His Val Glu Glu Leu Gly Lys Leu Thr Asn Leu Arg
675 680 685
Lys Leu Ser Ile Lys Leu Asp Thr Gly Gly Ile Glu Gly Asn Lys Leu
690 695 700
Glu Glu Ser Lys Glu Lys Leu Val Ser Ser Leu Cys Lys Leu Asp Glu
705 710 715 720
Cys Gly Leu Arg Ser Leu Ser Ile His Tyr Tyr Leu Arg Glu Lys Asp
725 730 735
Gly Glu Glu Pro Phe Leu Pro Ala Leu Gly Cys Ile Gln Glu Val Cys
740 745 750
Val Tyr Gly Gln Asp Ile Ser Arg Ile Ser Arg Trp Leu Ala Ser Leu
755 760 765
Pro Asn Leu His Arg Leu Phe Phe Asn Asp Pro Lys Met Lys Ile Glu
770 775 780
Gln Gln Asp Ile Glu Met Ile Gly Leu Ile Pro Asn Leu Met Asp Leu
785 790 795 800
Thr Leu Tyr Leu Arg Ile Thr Asp Asp Ala Met Pro Arg Leu Ile Ile
805 810 815
Lys Arg Glu Gly Phe Gln Gln Leu Gln Arg Phe Glu Leu Ser Arg Ile
820 825 830
Arg Met Gly Asp Leu Met Phe Glu Pro Gly Ala Met Pro Arg Leu Lys
835 840 845
Glu Leu Ile Leu His Asn Phe Ile Glu Lys Pro Lys Ser Gly Ala Val
850 855 860
Asp Phe Asp Phe Gly Ile Gln His Leu Ser Ser Leu Ala Arg Leu Thr
865 870 875 880
Val Gly Leu Leu Cys Val Gly Ser Thr Ala Ala Glu Val Glu Ala Ala
885 890 895
Glu Asp Ala Phe Lys Ser Met Ala Glu Ala Asn Pro Asn Arg Pro Ile
900 905 910
Leu Glu Met Thr Arg Leu Cys Pro Gln His Met Leu Gln Asp Glu Gln
915 920 925
Ile Gly Met Thr Gly Ser Ala Thr Thr Pro Ala Val Lys Ser
930 935 940
<210> 39
<211> 2826
<212> DNA
<213> 栽培一粒小麦
<400> 39
atggcggaag ttctggtgag cgcatccaca ggggcaatgg gttccctcct gcggaagttg 60
ggagctatgt tgacggatga atacaagctg ctcaagaacg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgcg ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacacgagt cctccagttc cgaggctcat 300
ggcgtcacca agctcatgga caagtgcaag aacttgctgc cggatatcaa gacccgccgc 360
aggatcgcca aggaggtcaa agacatcaag aaagaaatta aggatgtcag cgacagattt 420
ttaaggtaca agattgacga ctcctcctcc tctatgcctg ctgcaaaaga caaggtcgac 480
cctcgacttc gtgcagtcta caaagacgcc gcagagctcg tcggaattga tggaccaaag 540
gatgaacttg tcaagtggct gaatgagaaa gagggccaat cacttaaatc tgtctctatt 600
gttggatatg gagggttggg caagaccacg ctcgccaacc agatccgggt caaccttgga 660
gcgactttcg actgcggggc ttttgtctcg atttcacgca agcctgacat gaaggcgata 720
ttaagatcta tattatcgca aatcaccaag aaagacgagg cttgccctag attagatgat 780
atacaactca tcatcgacaa gattcgagaa ttcctccaag acacaaggta ctttatcata 840
attgatgata tatgggagtt aggaacatgg gaaactttga aatgtgcatt tgtcaagaat 900
acattgggca gcagaataat tatcacaaca cgtatagttg atgttgccaa atcttgctct 960
ccttctagtg aagatcttgt ctatgagatg aaaccactca gtgaggctga ctcgaagaaa 1020
ttgtttttca agaggatatt tggctgtgaa gaaagctgtc ctgatagctt gaaagaagct 1080
gccaatgata ttttgaagaa atgccgtggt ctacctctgg ccatcaatgc catttctagc 1140
ctgttggcca caacaaggga aacaaaagaa gagtgggatc gggtgcgaca ttcaatacgc 1200
tcttcaaagg tcaaaagtga tataatagag accatgaatt acatattgtc tcttagttat 1260
tttgatcttc cccaccacct aagaagctgc ttattgtacc tggctctgtt tcctgaagac 1320
caattgattg aaaggaagcg gttggtacgc agatggattt ctgaaggctt tatccatgga 1380
gaaagtggac aagctctcat ggaattagga gaggaatatt ttcaccagct tgtgaacaga 1440
agtctaatac agcccggcaa catagggtat gacggcaagg cggagtattg ccgagtccat 1500
gacaccatcc ttgatttcct aattgacaaa tcctctgaag agaacatgtg tactgtcctg 1560
aagaaacaat gcaagcctaa tggcatagtt cgtcggctct ctctaatggg gaatgaggat 1620
gaagaaatca tggagcaatt ggatctgtca cacgctcggt caatcagtgc ttttggggat 1680
atcaagctat tgccttcgct tgggaggtca aaatgcttgc gcgtgctaga cttgcaaggc 1740
tgctatcagt tgaaaaatca tcatatcaag gatattgaaa gactctatca gctgagatac 1800
ttggatatct cttttacagg aatcacggag cttcccaggc aaattgggga gttgctgtat 1860
ctagagacgc tagttacatc ctatggatta cgtgagcttc ctgaaagcac aagtcggcta 1920
caacgactgg cacgtttgtt tgtttaccac ggttgtaagc ttccagatgg gttgggaaac 1980
ttggtaaact tgcaagagct tgagtgcgtt gatgccttgc agttgaagca tgtggaagag 2040
ctcggcaaac taacaaatct gagaaagctg agtattaaat tggacactgg tgggattgaa 2100
ggcaacaaat tagaagagtc caaggaaaag ctggtgtcct ctctctgtaa actggacgaa 2160
tgcggcctcc gttccctgag tattcattat tatctgagag aaaaggatgg agaagagccg 2220
ttcctccctg ctttgggttg tatccaggag gtttgtgtat atggacaaga tatcagccgg 2280
attagcagat ggctcgcgtc acttcccaac ctacacaggt tgtttttcaa cgatcccaaa 2340
atgaagatag agcagcagga tattgagatg attggattga tacccaatct gatggatttg 2400
acactgtatt tacgcataac agacgacgct atgccgaggc tcatcatcaa gcgtgaagga 2460
tttcaacagt tgcagaggtt tgagctttcc cgtattcgta tgggggattt aatgtttgaa 2520
ccgggggcta tgccgaggct caaggagctt atactccata acttcatcga aaagcccaaa 2580
tctggtgcgg tcgatttcga ctttggcatc caacacctct ccagccttgc tcgcctcact 2640
gtcggcttat tatgcgttgg ctcgacggcg gcggaagtag aggctgcgga ggatgctttc 2700
aagagcatgg ccgaggcgaa ccccaaccgt ccaatactgg agatgacaag actttgtccg 2760
cagcatatgt tacaagacga gcaaataggt atgacaggct cagctacaac gccggccgtc 2820
aagtcc 2826
<210> 40
<211> 5847
<212> DNA
<213> 普通小麦
<400> 40
atggcggaag ttctggtgag cgcatccaca ggtgcaatgg gttccctcct gcggaagttg 60
ggagctatgt tgacggatga atataagctg ctcaagaatg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgca ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacacgagt cctcctgttc cgaggcggct 300
catggcgtcg ccaagctcat ggacaagtgc aagaacttgc tgccggacat caagacccgc 360
cgcaggatcg ccaaggaggt caaagacatc aagaaacaaa ttaaggatgt cagcgacaga 420
ttttcaaggt accagatctg gttctcttta cagtactatt agtacctacc atcagatttg 480
tgaccgattt gttgccactc aaggtacaag attgacgagt cctcctcctc tatgcccgca 540
aaagagaagg tcgaccctcg acttcgtgca gtctacaaag atgtcacaga gctcgtcgga 600
attgacggac caaaagatga acttgtcaag tggctgaatg agaaagaggg ccaatcactt 660
aaatctgtct ctattgttgg atatggaggg ttgggcaaga ccacgctcgc caaccagatc 720
cgggtcaacc ttagagcgtc cttcgactgc ggggcttttg tctcgatttc acgcaagcct 780
gacatgaagg ctatattaag atctatctta tcacaaatca ccaagaaaga cgatgcttac 840
tctagattag atgatataca actcatcatc gacaagattc gagaattcct ccaagacaca 900
aggtatgtat gtgagatctg ctacaaacta acattgctta attagctcat gtataatccg 960
ttgcctagaa ttttgctaat gaagattgct ttgtgtgatt tgcattcgcc ctcagcttgt 1020
atgactgcgt ttaaaatttg aagtcattgt tttctgcaac tgatatcgat ctgttcacta 1080
taagatatag ctaaaatctg catgccgatt tctggcgcca cacatcttct aaggacccaa 1140
agcacttgca ttcgcagacc actgttaccg gatatatgca gtacattagg tttgtgatta 1200
gccactttaa gaacgtaggg tttctagagg ctataaacct acggttatca gtattataat 1260
cctgactgga tatcaaacat ccgttgaaga atctataccc ataacacaaa aagttaaaag 1320
ggaagaatta atcatagtca gatcggacca gcctgactag cgtaacataa tgataaccgt 1380
agtttgttgc ttctttcctg gtttcgtagc aatttgcatg acgaattgag gcgcagaccc 1440
accactcaat atgtggtcct accaatcatg aggaaataac ttgttaccaa aaagacaaaa 1500
gaaaataggt aatccccacc attgtttcca tatctatcct cttgccaaca tgctattttt 1560
ttttgagcat cagtacagac acaagcgctc atatacacgt gcgtacactc acccctatga 1620
acgcacacac gcatacccta cccctatgag cacctccgag agtctgagtc gtcttgccaa 1680
catgctattg gcagtggcaa aacattgtat cttgccactg gcaacagctg cctcgactcg 1740
gcctgagcat ctcatcaagg aattcacaat ttttcctctt ggttgttaaa tcttgctctt 1800
ttttgtttgc ctcctatgga aatgtttttt cccttatatt gcgaccaaat tgtatctaag 1860
agcaactcca acgcgcctga cggagttttt gtccgctttt tgtccgtttg gatcggccga 1920
gcggacgggc atgtccggtt ttgccattgg gtgcgtgggc gcgcccaata tggcctacgc 1980
attagtccgc atgggcatta aaaaaaatta aaagcttaaa ataattaaac ataaataaaa 2040
atgcatatta aaaccggtca aagtccacat aattaacata aaacattaat aaataaacta 2100
ccgccctagc ccgcaaccct aggcatcgtc ctcgtcgtcg gagcgtgtga aatcaaagaa 2160
tgctggcggc ccagaccacg ggaaggccag ctggtaggct accaatgccg cctgctgcag 2220
cggtggcttt gcctggcacc ccgcgcgtct gctcctccac gcacgcccaa cgaagccggc 2280
gctcctcctc ctcgagctcc cgctgcatgc ggacctggct gtcctcccat tcctcctcca 2340
acgtgtggtt cttccatgcc gcgaggtagg ccgcctcctc cgtcgcgccc agccaaacgg 2400
tcagcgcgct cacccactcc cggacgacgt cctgccattg gtagacgctg gtgggcgcgg 2460
gtggtgccga cgctggcgcg ggcggttacg atggggccgg tggtggtggc aggtagacgt 2520
tgtcgctcgc agccgacaac tgcagtgcgt cctcgaggcc aggccaccag gagtccccgg 2580
gctgggactc ctcaatggcg cgctgggtcg cctcctccaa cgcctcgagg aacgccgcct 2640
cttcctcctc accctactgc ttgaccaccg gcggtggcag tggagggcgt gcttcgagct 2700
ggacggcgcc gtggcggcac acatcgtgct cggtggtgaa ccggacgtcc cagttagggg 2760
agtctagcgt gtaggcgggg tcgcagaact gctccgacgt caggagagca cacccgtctc 2820
ggatcttctt ggctcgcgga cgttcaaacc acgccacgga cgacaccgaa atgcggtggg 2880
ggtcgaggtg ccagccgtgg gggaggttca cgtccagcca cgacagtggg acgcggtact 2940
cctagtggta ctgcacggtc ccgtggcgct gtcgccctcc cgtcccatga cgccgacacc 3000
aatgcgcacc ccttcgtcgg cggcctcacc ggtccttgcg ctgagccagc ctcgtggtcg 3060
tgcttcccct tgcccttgcc tttgccgaag atacccatga tgactagggt tttctgtcgt 3120
cggtgaggga gcagatgtgg actggagtgg gaggagaagt ggataaggcc ggccccgccg 3180
caccattgca attaaaaagg atgcgatttg gacacttcta gtcactgcca tgcgggcccg 3240
aggtaggtgg gctgcattga ctatgactgc ggtggtagtt gatagtcgac acgcgggcat 3300
gaggcggacg cgcagcacgt ccatctcgtg ttcgtgtgga cgcaaatcag gtgcaaattt 3360
gggttggaaa tgtgtcacat ggacaccgag cggacgtggt ttgggtttgt gtccgcgtgt 3420
tgggctggcg ttttgtccat ttcgactcaa acggacacgc gcggaccaaa tgcgccggcg 3480
cgttggagtt gcttgtacct agtactatgt gtggagttgt tatctttgag tttcttccca 3540
atttgtagtt gtgtattatt gtgagatgct ctgaggattt cacttatcgt acaagtatgt 3600
tttagataat gacatcttta ttgatgttag acaattacat caaggtgtat tatatctagg 3660
atgcacacag atgtgacatt gtttcgggat tttacgttac ttgataggta ttactataaa 3720
tatctaatga gctctaatgc gtatattcat aaaactgtgt tgcaggtact tcatcataat 3780
tgatgatata tgggagttag gaacatggga aactttgaaa tgtgcatttg tcaagaatac 3840
attgggcagc agaataatta tcacaacacg tatagttgat gttgccaaat cttgctctcc 3900
ttctagtgaa gatcttgtct atgagatgaa accactcagt gaggctgact caaagaaatt 3960
gtttttcaag aggatatttg gctgtgaaga aagctgtcct gatagcttga aagaagctgc 4020
caatgatatt ttgaaaaaat gccgtggtct acctctggcc atcaatgcca tttctagctt 4080
gttggccaca acaagggaaa caaaagaaga gtgggatcgg gtgcgacatt caatacactc 4140
ttcaaaggtc aaaagtgata taatagagac catgaattac atattatctc ttagttattt 4200
tgatcttccc caccacctaa gaagctgctt attgtacctg gctctgtttc ctgaagacca 4260
attgattgga aggaagcggt tagtacgcag atggatttct gaaggcttta tccatggaga 4320
aagtggacaa gatctcatgg aattaggaga ggaatatttt caccagcttg taaacagaag 4380
tctaatacag cccgactaca tagggtatga cggcaagacg gagtattgcc gagtccatga 4440
caccatcctt gatttcctaa ttgacaaatc ctccgaagag aacatgtgta ccgtcctaaa 4500
gaaacaatgc aagcctaatg gcatagttcg tcggctctct ctaatgggga atgaggatga 4560
agaaatcgtg gagcaattgg atctgtcaca cgctcggtca atcagtgctt ttggggatat 4620
caagctattg ccttcgcttg ggaggtcaaa atgcttgcgc gtgctagact tgcaaaactg 4680
cggtcagttg agaaatcatc atatcaagga cattgaaaga ctctatcagc tgagatactt 4740
ggatatctct tttacaggaa tcacggagct tcccaggcaa attggggagt tgctgtatct 4800
agagacgcta gttacatccg atggattacg tgagcttcct gaaagcacaa gtcggctaca 4860
acgactggca cgtttgattg ttggctgcga ttgtaagctt ccagatgggt tgggaaactt 4920
gatgaacttg caagagcttg actgcgttgg tgccttgcat ttgaagcatg cggaagagct 4980
cggcaaacta acaaatctga gaaagctgaa gattaacttg tacactcatg ggattgaagg 5040
caacaaatta gaagagtcca aggaaaagct ggtgtcctct ctctgtaaac tggacgaatg 5100
cggcctccgt tccctgagta ttgattatta tctgagagaa aaggatggag aagagccgtt 5160
cctccctgct ttaggttgta tcgaggaggt ttttgtatat gaacaagata tcagccggat 5220
tagcagatgg ctcgcgtcac ttcccaacct acacaggttg gtttcctacg atccgaagat 5280
agagcagcag gatattgaga tgattggatt gatacccaat ctgatcgatt tgacactgtc 5340
tttaccggga acagacgacg ctgggcggtt catcatcacg cgtgaaggat ttcaacagtt 5400
gcagagtttt gagctttccg gtagtcgtat gggagtttta tttgaaccgg gggctatgcc 5460
gaggctcaag gagcttatac tcgacgactt catcagaaag cccaaatctg ctgcggtcga 5520
tttcgacttt ggcatccaac gcctctccag ccttgctcgc ctcactgtca gcttagcctg 5580
ctatcgctcg acggcggcag aagtagaggc tacggaggat gctttcaaga gcatggccga 5640
ggcgaacccc aaccgtccaa tactggagat gacaagactt tatccgcagc atatggtacg 5700
agacgagcaa atagatatgg caggctcagg tactattaac aagtttgtac tttttgtctt 5760
ggtgagcagt agatcaaaag atgtgcatat gcttaattct ttttccggtc atctatttca 5820
gctacaacgc cggccgtcaa gtcctag 5847
<210> 41
<211> 938
<212> PRT
<213> 普通小麦
<400> 41
Met Ala Glu Val Leu Val Ser Ala Ser Thr Gly Ala Met Gly Ser Leu
1 5 10 15
Leu Arg Lys Leu Gly Ala Met Leu Thr Asp Glu Tyr Lys Leu Leu Lys
20 25 30
Asn Val Arg Gly Asp Ile Lys Phe Leu Lys Asp Glu Leu Glu Val Met
35 40 45
Cys Ala Phe Leu Leu Lys Met Ser Asp Val Glu Glu Pro Asp Glu Pro
50 55 60
Thr Lys Leu Arg Val Thr Ala Val Arg Glu Met Ser Tyr Lys Ile Glu
65 70 75 80
Asp Asn Ile Asp Lys Phe Met Val Leu Val Glu His Glu Ser Ser Cys
85 90 95
Ser Glu Ala Ala His Gly Val Ala Lys Leu Met Asp Lys Cys Lys Asn
100 105 110
Leu Leu Pro Asp Ile Lys Thr Arg Arg Arg Ile Ala Lys Glu Val Lys
115 120 125
Asp Ile Lys Lys Gln Ile Lys Asp Val Ser Asp Arg Phe Ser Arg Tyr
130 135 140
Lys Ile Asp Glu Ser Ser Ser Ser Met Pro Ala Lys Glu Lys Val Asp
145 150 155 160
Pro Arg Leu Arg Ala Val Tyr Lys Asp Val Thr Glu Leu Val Gly Ile
165 170 175
Asp Gly Pro Lys Asp Glu Leu Val Lys Trp Leu Asn Glu Lys Glu Gly
180 185 190
Gln Ser Leu Lys Ser Val Ser Ile Val Gly Tyr Gly Gly Leu Gly Lys
195 200 205
Thr Thr Leu Ala Asn Gln Ile Arg Val Asn Leu Arg Ala Ser Phe Asp
210 215 220
Cys Gly Ala Phe Val Ser Ile Ser Arg Lys Pro Asp Met Lys Ala Ile
225 230 235 240
Leu Arg Ser Ile Leu Ser Gln Ile Thr Lys Lys Asp Asp Ala Tyr Ser
245 250 255
Arg Leu Asp Asp Ile Gln Leu Ile Ile Asp Lys Ile Arg Glu Phe Leu
260 265 270
Gln Asp Thr Arg Tyr Phe Ile Ile Ile Asp Asp Ile Trp Glu Leu Gly
275 280 285
Thr Trp Glu Thr Leu Lys Cys Ala Phe Val Lys Asn Thr Leu Gly Ser
290 295 300
Arg Ile Ile Ile Thr Thr Arg Ile Val Asp Val Ala Lys Ser Cys Ser
305 310 315 320
Pro Ser Ser Glu Asp Leu Val Tyr Glu Met Lys Pro Leu Ser Glu Ala
325 330 335
Asp Ser Lys Lys Leu Phe Phe Lys Arg Ile Phe Gly Cys Glu Glu Ser
340 345 350
Cys Pro Asp Ser Leu Lys Glu Ala Ala Asn Asp Ile Leu Lys Lys Cys
355 360 365
Arg Gly Leu Pro Leu Ala Ile Asn Ala Ile Ser Ser Leu Leu Ala Thr
370 375 380
Thr Arg Glu Thr Lys Glu Glu Trp Asp Arg Val Arg His Ser Ile His
385 390 395 400
Ser Ser Lys Val Lys Ser Asp Ile Ile Glu Thr Met Asn Tyr Ile Leu
405 410 415
Ser Leu Ser Tyr Phe Asp Leu Pro His His Leu Arg Ser Cys Leu Leu
420 425 430
Tyr Leu Ala Leu Phe Pro Glu Asp Gln Leu Ile Gly Arg Lys Arg Leu
435 440 445
Val Arg Arg Trp Ile Ser Glu Gly Phe Ile His Gly Glu Ser Gly Gln
450 455 460
Asp Leu Met Glu Leu Gly Glu Glu Tyr Phe His Gln Leu Val Asn Arg
465 470 475 480
Ser Leu Ile Gln Pro Asp Tyr Ile Gly Tyr Asp Gly Lys Thr Glu Tyr
485 490 495
Cys Arg Val His Asp Thr Ile Leu Asp Phe Leu Ile Asp Lys Ser Ser
500 505 510
Glu Glu Asn Met Cys Thr Val Leu Lys Lys Gln Cys Lys Pro Asn Gly
515 520 525
Ile Val Arg Arg Leu Ser Leu Met Gly Asn Glu Asp Glu Glu Ile Val
530 535 540
Glu Gln Leu Asp Leu Ser His Ala Arg Ser Ile Ser Ala Phe Gly Asp
545 550 555 560
Ile Lys Leu Leu Pro Ser Leu Gly Arg Ser Lys Cys Leu Arg Val Leu
565 570 575
Asp Leu Gln Asn Cys Gly Gln Leu Arg Asn His His Ile Lys Asp Ile
580 585 590
Glu Arg Leu Tyr Gln Leu Arg Tyr Leu Asp Ile Ser Phe Thr Gly Ile
595 600 605
Thr Glu Leu Pro Arg Gln Ile Gly Glu Leu Leu Tyr Leu Glu Thr Leu
610 615 620
Val Thr Ser Asp Gly Leu Arg Glu Leu Pro Glu Ser Thr Ser Arg Leu
625 630 635 640
Gln Arg Leu Ala Arg Leu Ile Val Gly Cys Asp Cys Lys Leu Pro Asp
645 650 655
Gly Leu Gly Asn Leu Met Asn Leu Gln Glu Leu Asp Cys Val Gly Ala
660 665 670
Leu His Leu Lys His Ala Glu Glu Leu Gly Lys Leu Thr Asn Leu Arg
675 680 685
Lys Leu Lys Ile Asn Leu Tyr Thr His Gly Ile Glu Gly Asn Lys Leu
690 695 700
Glu Glu Ser Lys Glu Lys Leu Val Ser Ser Leu Cys Lys Leu Asp Glu
705 710 715 720
Cys Gly Leu Arg Ser Leu Ser Ile Asp Tyr Tyr Leu Arg Glu Lys Asp
725 730 735
Gly Glu Glu Pro Phe Leu Pro Ala Leu Gly Cys Ile Glu Glu Val Phe
740 745 750
Val Tyr Glu Gln Asp Ile Ser Arg Ile Ser Arg Trp Leu Ala Ser Leu
755 760 765
Pro Asn Leu His Arg Leu Val Ser Tyr Asp Pro Lys Ile Glu Gln Gln
770 775 780
Asp Ile Glu Met Ile Gly Leu Ile Pro Asn Leu Ile Asp Leu Thr Leu
785 790 795 800
Ser Leu Pro Gly Thr Asp Asp Ala Gly Arg Phe Ile Ile Thr Arg Glu
805 810 815
Gly Phe Gln Gln Leu Gln Ser Phe Glu Leu Ser Gly Ser Arg Met Gly
820 825 830
Val Leu Phe Glu Pro Gly Ala Met Pro Arg Leu Lys Glu Leu Ile Leu
835 840 845
Asp Asp Phe Ile Arg Lys Pro Lys Ser Ala Ala Val Asp Phe Asp Phe
850 855 860
Gly Ile Gln Arg Leu Ser Ser Leu Ala Arg Leu Thr Val Ser Leu Ala
865 870 875 880
Cys Tyr Arg Ser Thr Ala Ala Glu Val Glu Ala Thr Glu Asp Ala Phe
885 890 895
Lys Ser Met Ala Glu Ala Asn Pro Asn Arg Pro Ile Leu Glu Met Thr
900 905 910
Arg Leu Tyr Pro Gln His Met Val Arg Asp Glu Gln Ile Asp Met Ala
915 920 925
Gly Ser Ala Thr Thr Pro Ala Val Lys Ser
930 935
<210> 42
<211> 2814
<212> DNA
<213> 普通小麦
<400> 42
atggcggaag ttctggtgag cgcatccaca ggtgcaatgg gttccctcct gcggaagttg 60
ggagctatgt tgacggatga atataagctg ctcaagaatg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgca ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacacgagt cctcctgttc cgaggcggct 300
catggcgtcg ccaagctcat ggacaagtgc aagaacttgc tgccggacat caagacccgc 360
cgcaggatcg ccaaggaggt caaagacatc aagaaacaaa ttaaggatgt cagcgacaga 420
ttttcaaggt acaagattga cgagtcctcc tcctctatgc ccgcaaaaga gaaggtcgac 480
cctcgacttc gtgcagtcta caaagatgtc acagagctcg tcggaattga cggaccaaaa 540
gatgaacttg tcaagtggct gaatgagaaa gagggccaat cacttaaatc tgtctctatt 600
gttggatatg gagggttggg caagaccacg ctcgccaacc agatccgggt caaccttaga 660
gcgtccttcg actgcggggc ttttgtctcg atttcacgca agcctgacat gaaggctata 720
ttaagatcta tcttatcaca aatcaccaag aaagacgatg cttactctag attagatgat 780
atacaactca tcatcgacaa gattcgagaa ttcctccaag acacaaggta cttcatcata 840
attgatgata tatgggagtt aggaacatgg gaaactttga aatgtgcatt tgtcaagaat 900
acattgggca gcagaataat tatcacaaca cgtatagttg atgttgccaa atcttgctct 960
ccttctagtg aagatcttgt ctatgagatg aaaccactca gtgaggctga ctcaaagaaa 1020
ttgtttttca agaggatatt tggctgtgaa gaaagctgtc ctgatagctt gaaagaagct 1080
gccaatgata ttttgaaaaa atgccgtggt ctacctctgg ccatcaatgc catttctagc 1140
ttgttggcca caacaaggga aacaaaagaa gagtgggatc gggtgcgaca ttcaatacac 1200
tcttcaaagg tcaaaagtga tataatagag accatgaatt acatattatc tcttagttat 1260
tttgatcttc cccaccacct aagaagctgc ttattgtacc tggctctgtt tcctgaagac 1320
caattgattg gaaggaagcg gttagtacgc agatggattt ctgaaggctt tatccatgga 1380
gaaagtggac aagatctcat ggaattagga gaggaatatt ttcaccagct tgtaaacaga 1440
agtctaatac agcccgacta catagggtat gacggcaaga cggagtattg ccgagtccat 1500
gacaccatcc ttgatttcct aattgacaaa tcctccgaag agaacatgtg taccgtccta 1560
aagaaacaat gcaagcctaa tggcatagtt cgtcggctct ctctaatggg gaatgaggat 1620
gaagaaatcg tggagcaatt ggatctgtca cacgctcggt caatcagtgc ttttggggat 1680
atcaagctat tgccttcgct tgggaggtca aaatgcttgc gcgtgctaga cttgcaaaac 1740
tgcggtcagt tgagaaatca tcatatcaag gacattgaaa gactctatca gctgagatac 1800
ttggatatct cttttacagg aatcacggag cttcccaggc aaattgggga gttgctgtat 1860
ctagagacgc tagttacatc cgatggatta cgtgagcttc ctgaaagcac aagtcggcta 1920
caacgactgg cacgtttgat tgttggctgc gattgtaagc ttccagatgg gttgggaaac 1980
ttgatgaact tgcaagagct tgactgcgtt ggtgccttgc atttgaagca tgcggaagag 2040
ctcggcaaac taacaaatct gagaaagctg aagattaact tgtacactca tgggattgaa 2100
ggcaacaaat tagaagagtc caaggaaaag ctggtgtcct ctctctgtaa actggacgaa 2160
tgcggcctcc gttccctgag tattgattat tatctgagag aaaaggatgg agaagagccg 2220
ttcctccctg ctttaggttg tatcgaggag gtttttgtat atgaacaaga tatcagccgg 2280
attagcagat ggctcgcgtc acttcccaac ctacacaggt tggtttccta cgatccgaag 2340
atagagcagc aggatattga gatgattgga ttgataccca atctgatcga tttgacactg 2400
tctttaccgg gaacagacga cgctgggcgg ttcatcatca cgcgtgaagg atttcaacag 2460
ttgcagagtt ttgagctttc cggtagtcgt atgggagttt tatttgaacc gggggctatg 2520
ccgaggctca aggagcttat actcgacgac ttcatcagaa agcccaaatc tgctgcggtc 2580
gatttcgact ttggcatcca acgcctctcc agccttgctc gcctcactgt cagcttagcc 2640
tgctatcgct cgacggcggc agaagtagag gctacggagg atgctttcaa gagcatggcc 2700
gaggcgaacc ccaaccgtcc aatactggag atgacaagac tttatccgca gcatatggta 2760
cgagacgagc aaatagatat ggcaggctca gctacaacgc cggccgtcaa gtcc 2814
<210> 43
<211> 5918
<212> DNA
<213> 普通小麦
<400> 43
atggcggaag ttctggtgag cgcatccaca ggggcaatgg gttccctcct gcggaagttg 60
ggagctatgt tgacggatga atacaagctg ctcaagaacg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgcg ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacaggaac acgggtcctc ctgttccgag 300
gcggctcatg gcgtcgccaa gctcatggac aagtgcaaga acttgctgcc ggacatcaag 360
gcccgccgca ggatcgccaa ggaggtcaaa gacatcaaga aagaaattaa ggatgtcagc 420
gacagatttt caaggtacca gatcttgttc tccttacagt actattagta cctaccatca 480
gatttgtgac cgatttgttg ccactcaagg tacaagattg acgactcctc ctcctctatg 540
ccggcaaaag acaaggtcga ctctcgactt cgtgcagtct acaaagacgc cgcagagctc 600
gtcggaattg atggaccaaa ggatgaactt gtcaagtggc tgaatgagaa agaaggccaa 660
tcactgaaat ctgtctctat tgttggatat ggagggttgg gcaagaccac gctcgccaac 720
cagatccggg tcaaccttgg agcgaccttc gactgcgggg cttttgtctc gatttcacgc 780
aagcctgaca tgaaggcgat attaagatct atattatcac aaatcaccaa gaaagacgat 840
gcttgctcta gattagatga tatacaactc atcatcgaca agattcgaga attcctccaa 900
gacacaaggt atgtatgtga gatctgctac gaagtaacat tgcttaatta gctcatgcat 960
aatcggttgc ctagaatttt gctaatgaag attgctttgt gtgatttact ttcgtcctca 1020
acttgtatga ctgcgcttaa actttcaagt cattgtttct tgcaacttat gtcgatctgt 1080
tcactaatta agatatagct aaaatgtgtt tgccgatttc tgtcgccaca cattttctaa 1140
tttgattgcc acaagtttgt taaacttgcg gctatgttct gtttattaaa taattgtgac 1200
atggcgtcag tcaaaacaat aaagtacatg caattccctc tcttttgtac aagataaata 1260
tccgcacgca ctacatgact gcaaatatat ctatcttcta aggacccaaa gcacttgcat 1320
gtgcagacca ttgttaccgg atatgcagta cattaggttt gtgattagcc actttaagaa 1380
catagggttt ctagaggcta taaacctacg gttatcagta ttataatctt gaatggatat 1440
caaacatccg ttgaagaatc tatgcccata acacaaaaag agaaaaggga agaattaatc 1500
atagtcagat cggaccagcc tgactagcgt aacataatga caacagtagg ttgttgcgtc 1560
tttcctggtt tcgtagcaat ttgcatgacg aattgaggcg cagagccacc actcaatatg 1620
tggtcctacc aatcatgagg aaataacttg ttaccaaaat gacaaaagaa aataggtagt 1680
ccccatcatt gtttcaatat ctatcctctt gcgaacatgc tattggcagt ggcaaaacat 1740
tgtaccttgc cactggcaac agctgccttt actcggcctg agcattcata tttttttcct 1800
cttggttgtt aaatcttgct atttttcgct tgccttctat ggcaatcttt ttttttctta 1860
tattgccacc aaattgtatc taagagcaac tccaacacgc cgacgcaatt tttgtccatt 1920
tagatcggcc aagcggacgg ggtgtctggt tttgcctttg ggtggtgcgc ctacgcatta 1980
gtccgcgtgg gcatttaaaa aacttaaaaa gcttaaaata attaaatata aataaaaatg 2040
catattaaag cccgtcaaag tccacataat taaacataat acattaatat attaataaaa 2100
aacagccgcc ccagcccgca gccctaggcg tcttcgtcgg aggagtgggt gaggtcgaag 2160
aatgccggca gctcagccca cgggaaggcc tgctggtggg cagccagcac cgcctgctcc 2220
gctgtctgcg gcggcggcgg ccctacctgg caccgcgcac gcctcctcct cgcgctcctg 2280
ccgctcctcc tcctcatcgc acgcccaacg aagttttggc gctcctcccc ctcgggttcc 2340
cgctgcatgc ggacctggcc gtcgtaccgc tcctcctcca gcgtgcggtc cttccacgcc 2400
acgaggtagg ccgcctcctc ctccatcacg cacagccaaa tgggcggctt gctcatccgc 2460
ttgcagacga tgccctgcca ttggtaaacg ccggtgggcg cgggtggtgc tagcgctggt 2520
gcaggcggtg acgatgtggc tggtggtccc ggcaggtaga cactatagct ggcggccgat 2580
agctgcagcg tgtcatcgag gccagaccac taggagtcct cgcactaaga ctcctcggtg 2640
acgtgatggg ccccctcctc caatgccttg aggaacgccg cctcctcctc aacctactat 2700
ttgaccaccg gtggcggcgg tggagggcag gcttcgagct ggacggcgtc gcggaggcgc 2760
acatggtgct cgatggtgaa ccagacttct cagttagggg agtctggcct cgatgggtgg 2820
agtcgcgacg ctgctctggc gtcaggagag cacgccgggc ccgaatctcg ttggcgcgcg 2880
cacgctcgga ctgtggcacc gccggcactg gaatgtggtg ggggtcgagg tgccagccgt 2940
gggggaagtt cacgtccggc cacggcagtg agacgcggta ctcccggtgg tactacgcgt 3000
ggtgcaccgg gatgtactgg tggtcccgtg gcgctgccgc cctcccatcc cgcagcgccg 3060
acaccaatgc gaaccccttc gctgccggcc tcaccggccc ttgcaacgag ccggcctcgt 3120
ggtcgtgctt gccttgccct tacctttgcc aaagaaaccc atgacggcta gggttttctg 3180
tcaccggcga gggagcagat gtggactaga gtgggaggag aagtggatga ggcccgcccc 3240
gccgcaccct tgcaattaaa aagggcgtga tccggacgtt tctagtcact gccatgcggg 3300
cccgaggtag gtgggcgcca ttgactacgt ctgtggtggt agttggctgg cagacacgcc 3360
agcccgaggc gcacaccgag cggacgcgca acacgtccgt ctcgtgttcg tgtgaacgca 3420
aatcaggcac aaatttgggc tggaaatgcg tctacatgga tgccgaacgg acgtgatttg 3480
ggtttgtgtc cgcacgttgg gccggcgttt ttgtgccttt cgaccctaat ggacacacac 3540
ggaccaaatc cgttggcgtg ttagagttgc ttgtacctag tactactatg tgtggagttg 3600
ttatctttga gtttctcccc catctgtagc tgtgtattat tgtgagatgc tctcaggatt 3660
tcacttattt tagataatga caactttctt gatgctagac aattacatca aggtgtatta 3720
tatctaggat gcacacagat gtgagattgt ttcaggattt taggttactt ggtaggtgtt 3780
actataaata tctaatgagc tctaatgtgt atgttcatga aactgtgttg caggtacttt 3840
atcataattg atgatatatg ggagttagga acatgggaaa ctttgaaatg tgcatttgtc 3900
aagaatacat tgggcagcag aataattatc acaacacgta tagttgatgt tgccaaatct 3960
tgttctcctt ctagtgaaga tcttgtctat gagatgaaac cactcagtga ggctgactcg 4020
aagaaattgt ttttcaagag gatatttggc tgtgaagaaa gctgtcctga tagcttgaaa 4080
gaagctgcca atgatatttt gaaaaaatgc cgtggcctac ctctggccat caatgccatt 4140
tctagcgtgt tggtcacaac aagggaaaca aaagaagagt gggatcgggt gcgacattca 4200
atacgctctt caaaggtcaa aagtgatata atagagacca tgaattacat attgtctctt 4260
agttattttg atcttcccca ccacctaaga agctgcttat tgtacctggc tctgtttcct 4320
gaagaccaat tgattggaag gaagcggtta gtacgcagat ggatttctga aggctttatc 4380
catggagaaa gtggacaaga tctcatggaa ttaggagagg aatattttca ccagcttgtg 4440
aacagaagtc taatacagcc cggcaacata gggtatgacg gcaaggcaat gtattgccga 4500
gtccatgaca ccatccttga tttcctaatt gacaaatcct ctgaagagaa catgtgtact 4560
gtcctaaaga aacaatgcaa gcctaatggc atagttcgtc ggctctctct aatggggaat 4620
gaggatgaag aaatcgtgga gcaattggat ctgtcacacg ctcggtcaat cactgctttt 4680
ggggatatca agctattgcc ttcgcttggg aggtcaaaat gcttgcgcgt gctagacttg 4740
caagactgcg atcagttgga aaatcatcat atcaaggata ttgaaagact ctatcagcta 4800
agatacttgg atatctcttc tacaggaatc acggagcttc ccaggcaaat tggggagttg 4860
ctgtatctag agacgctagt tgcctatgga ttacgtgagc ttcctgaaag cacaagtcgg 4920
ctacaacgac tggcacgttt gtttgtttac tctggttgta agcttccagg tgggttggga 4980
aacttgataa acttgcaaga gcttgactgc gttgatgcct tgcatttgaa gcatgtggaa 5040
gagctcggca aactaacaaa tctgagaaag ctgagtatta aattggacac tggtgggatt 5100
gaaggcaaca aattagaaga gtccaaggaa aagctggtgt cctctctctg taaactggac 5160
gaatgcggcc tcctttccct gagtattgat tattatctga gagaaaagga tggagaagag 5220
ccgttcctcc ctgctttggg ttgtatccag gaggtttttg tatatggaca agatatcagc 5280
cggattagca gatggctcgc gtcacttccc aacctacaca ggttgctttt ggacgatccg 5340
aagatagagc agcaggatat tgagatgatt ggattgatac ccaatctgat cgatttgaca 5400
ctgccgcctc tatacaaaac agacgacgct gggcggctca tcatcaggcg tgaaggattt 5460
caacagttgc agaagtttga ggcttacaat actcgtatgg gggttttaat gtttgaaccg 5520
ggggctatgc cgaggctcaa ggagcttaaa ctccataact tcatcgaaaa gcccaaatct 5580
gctgcggtcg atttcgactt tggcatccaa cgcctctcca gccttgctcg cctcactgtc 5640
agcttatcct gcggtggctg gacggtggca gaagtagagg ctgcggagga tgctttcaag 5700
agcatggccg aggcgaaccc caaccgtcca atactggaga tgacaagata taatacgcag 5760
catatgttac aagacgagca aataggtatg acaggctcag gtactattaa ctagtttgta 5820
cttttgtctt ggtgagcagt agatcaaaag atgtgcatat gcttcaatct tttttccggt 5880
aatctatttc agctacaacg ccggccgtca agtcctag 5918
<210> 44
<211> 5918
<212> DNA
<213> 普通小麦
<400> 44
atggcggaag ttctggtgag cgcatccaca ggggcaatgg gttccctcct gcggaagttg 60
ggagctatgt tgacggatga atacaagctg ctcaagaacg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgcg ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacaggaac acgggtcctc ctgttccgag 300
gcggctcatg gcgtcgccaa gctcatggac aagtgcaaga acttgctgcc ggacatcaag 360
gcccgccgca ggatcgccaa ggaggtcaaa gacatcaaga aagaaattaa ggatgtcagc 420
gacagatttt caaggtacca gatcttgttc tccttacagt actattagta cctaccatca 480
gatttgtgac cgatttgttg ccactcaagg tacaagattg acgactcctc ctcctctatg 540
ccggcaaaag acaaggtcga ccctcgactt cgtgcagtct acaaagacgc cgcagagctc 600
gtcggaattg atggaccaaa ggatgaactt gtcaagtggc tgaatgagaa agaaggccaa 660
tcactgaaat ctgtctctat tgttggatat ggagggttgg gcaagaccac gctcgccaac 720
cagatccggg tcaaccttgg agcgaccttc gactgcgggg cttttgtctc gatttcacgc 780
aagcctgaca tgaaggcgat attaagatct atattatcac aaatcaccaa gaaagacgat 840
gcttgctcta gattagatga tatacaactc atcatcgaca agattcgaga attcctccaa 900
gacacaaggt atgtatgtga gatctgctac gaagtaacat tgcttaatta gctcatgcat 960
aatcggttgc ctagaatttt gctaatgaag attgctttgt gtgatttact ttcgtcctca 1020
acttgtatga ctgcgcttaa actttcaagt cattgtttct tgcaacttat gtcgatctgt 1080
tcactaatta agatatagct aaaatgtgtt tgccgatttc tgtcgccaca cattttctaa 1140
tttgattgcc acaagtttgt taaacttgcg gctatgttct gtttattaaa taattgtgac 1200
atggcgtcag tcaaaacaat aaagtacatg caattccctc tcttttgtac aagataaata 1260
tccgcacgca ctacatgact gcaaatatat ctatcttcta aggacccaaa gcacttgcat 1320
gtgcagacca ttgttaccgg atatgcagta cattaggttt gtgattagcc actttaagaa 1380
catagggttt ctagaggcta taaacctacg gttatcagta ttataatctt gaatggatat 1440
caaacatccg ttgaagaatc tatgcccata acacaaaaag agaaaaggga agaattaatc 1500
atagtcagat cggaccagcc tgactagcgt aacataatga caacagtagg ttgttgcgtc 1560
tttcctggtt tcgtagcaat ttgcatgacg aattgaggcg cagagccacc actcaatatg 1620
tggtcctacc aatcatgagg aaataacttg ttaccaaaat gacaaaagaa aataggtagt 1680
ccccatcatt gtttcaatat ctatcctctt gcgaacatgc tattggcagt ggcaaaacat 1740
tgtaccttgc cactggcaac agctgccttt actcggcctg agcattcata tttttttcct 1800
cttggttgtt aaatcttgct atttttcgct tgccttctat ggcaatcttt ttttttctta 1860
tattgccacc aaattgtatc taagagcaac tccaacacgc cgacgcaatt tttgtccatt 1920
tagatcggcc aagcggacgg ggtgtctggt tttgcctttg ggtggtgcgc ctacgcatta 1980
gtccgcgtgg gcatttaaaa aacttaaaaa gcttaaaata attaaatata aataaaaatg 2040
catattaaag cccgtcaaag tccacataat taaacataat acattaatat attaataaaa 2100
aacagccgcc ccagcccgca gccctaggcg tcttcgtcgg aggagtgggt gaggtcgaag 2160
aatgccggca gctcagccca cgggaaggcc tgctggtggg cagccagcac cgcctgctcc 2220
gctgtctgcg gcggcggcgg ccctacctgg caccgcgcac gcctcctcct cgcgctcctg 2280
ccgctcctcc tcctcatcgc acgcccaacg aagttttggc gctcctcccc ctcgggttcc 2340
cgctgcatgc ggacctggcc gtcgtaccgc tcctcctcca gcgtgcggtc cttccacgcc 2400
acgaggtagg ccgcctcctc ctccatcacg cacagccaaa tgggcggctt gctcatccgc 2460
ttgcagacga tgccctgcca ttggtaaacg ccggtgggcg cgggtggtgc tagcgctggt 2520
gcaggcggtg acgatgtggc tggtggtccc ggcaggtaga cactatagct ggcggccgat 2580
agctgcagcg tgtcatcgag gccagaccac taggagtcct cgcactaaga ctcctcggtg 2640
acgtgatggg ccccctcctc caatgccttg aggaacgccg cctcctcctc aacctactat 2700
ttgaccaccg gtggcggcgg tggagggcag gcttcgagct ggacggcgtc gcggaggcgc 2760
acatggtgct cgatggtgaa ccagacttct cagttagggg agtctggcct cgatgggtgg 2820
agtcgcgacg ctgctctggc gtcaggagag cacgccgggc ccgaatctcg ttggcgcgcg 2880
cacgctcgga ctgtggcacc gccggcactg gaatgtggtg ggggtcgagg tgccagccgt 2940
gggggaagtt cacgtccggc cacggcagtg agacgcggta ctcccggtgg tactacgcgt 3000
ggtgcaccgg gatgtactgg tggtcccgtg gcgctgccgc cctcccatcc cgcagcgccg 3060
acaccaatgc gaaccccttc gctgccggcc tcaccggccc ttgcaacgag ccggcctcgt 3120
ggtcgtgctt gccttgccct tacctttgcc aaagaaaccc atgacggcta gggttttctg 3180
tcaccggcga gggagcagat gtggactaga gtgggaggag aagtggatga ggcccgcccc 3240
gccgcaccct tgcaattaaa aagggcgtga tccggacgtt tctagtcact gccatgcggg 3300
cccgaggtag gtgggcgcca ttgactacgt ctgtggtggt agttggctgg cagacacgcc 3360
agcccgaggc gcacaccgag cggacgcgca acacgtccgt ctcgtgttcg tgtgaacgca 3420
aatcaggcac aaatttgggc tggaaatgcg tctacatgga tgccgaacgg acgtgatttg 3480
ggtttgtgtc cgcacgttgg gccggcgttt ttgtgccttt cgaccctaat ggacacacac 3540
ggaccaaatc cgttggcgtg ttagagttgc ttgtacctag tactactatg tgtggagttg 3600
ttatctttga gtttctcccc catctgtagc tgtgtattat tgtgagatgc tctcaggatt 3660
tcacttattt tagataatga caactttctt gatgctagac aattacatca aggtgtatta 3720
tatctaggat gcacacagat gtgagattgt ttcaggattt taggttactt ggtaggtgtt 3780
actataaata tctaatgagc tctaatgtgt atgttcatga aactgtgttg caggtacttt 3840
atcataattg atgatatatg ggagttagga acatgggaaa ctttgaaatg tgcatttgtc 3900
aagaatacat tgggcagcag aataattatc acaacacgta tagttgatgt tgccaaatct 3960
tgttctcctt ctagtgaaga tcttgtctat gagatgaaac cactcagtga ggctgactcg 4020
aagaaattgt ttttcaagag gatatttggc tgtgaagaaa gctgtcctga tagcttgaaa 4080
gaagctgcca atgatatttt gaaaaaatgc cgtggcctac ctctggccat caatgccatt 4140
tctagcgtgt tggtcacaac aagggaaaca aaagaagagt gagatcgggt gcgacattca 4200
atacgctctt caaaggtcaa aagtgatata atagagacca tgaattacat attgtctctt 4260
agttattttg atcttcccca ccacctaaga agctgcttat tgtacctggc tctgtttcct 4320
gaagaccaat tgattggaag gaagcggtta gtacgcagat ggatttctga aggctttatc 4380
catggagaaa gtggacaaga tctcatggaa ttaggagagg aatattttca ccagcttgtg 4440
aacagaagtc taatacagcc cggcaacata gggtatgacg gcaaggcaat gtattgccga 4500
gtccatgaca ccatccttga tttcctaatt gacaaatcct ctgaagagaa catgtgtact 4560
gtcctaaaga aacaatgcaa gcctaatggc atagttcgtc ggctctctct aatggggaat 4620
gaggatgaag aaatcgtgga gcaattggat ctgtcacacg ctcggtcaat cactgctttt 4680
ggggatatca agctattgcc ttcgcttggg aggtcaaaat gcttgcgcgt gctagacttg 4740
caagactgcg atcagttgga aaatcatcat atcaaggata ttgaaagact ctatcagcta 4800
agatacttgg atatctcttc tacaggaatc acggagcttc ccaggcaaat tggggagttg 4860
ctgtatctag agacgctagt tgcctatgga ttacgtgagc ttcctgaaag cacaagtcgg 4920
ctacaacgac tggcacgttt gtttgtttac tctggttgta agcttccagg tgggttggga 4980
aacttgataa acttgcaaga gcttgactgc gttgatgcct tgcatttgaa gcatgtggaa 5040
gagctcggca aactaacaaa tctgagaaag ctgagtatta aattggacac tggtgggatt 5100
gaaggcaaca aattagaaga gtccaaggaa aagctggtgt cctctctctg taaactggac 5160
gaatgcggcc tcctttccct gagtattgat tattatctga gagaaaagga tggagaagag 5220
ccgttcctcc ctgctttggg ttgtatccag gaggtttttg tatatggaca agatatcagc 5280
cggattagca gatggctcgc gtcacttccc aacctacaca ggttgctttt ggacgatccg 5340
aagatagagc agcaggatat tgagatgatt ggattgatac ccaatctgat cgatttgaca 5400
ctgccgcctc tatacaaaac agacgacgct gggcggctca tcatcaggcg tgaaggattt 5460
caacagttgc agaagtttga ggcttacaat actcgtatgg gggttttaat gtttgaaccg 5520
ggggctatgc cgaggctcaa ggagcttaaa ctccataact tcatcgaaaa gcccaaatct 5580
gctgcggtcg atttcgactt tggcatccaa cgcctctcca gccttgctcg cctcactgtc 5640
agcttatcct gcggtggctg gacggtggca gaagtagagg ctgcggagga tgctttcaag 5700
agcatggccg aggcgaaccc caaccgtcca atactggaga tgacaagata taatacgcag 5760
catatgttac aagacgagca aataggtatg acaggctcag gtactattaa ctagtttgta 5820
cttttgtctt ggtgagcagt agatcaaaag atgtgcatat gcttcaatct tttttccggt 5880
aatctatttc agctacaacg ccggccgtca agtcctag 5918
<210> 45
<211> 5918
<212> DNA
<213> 普通小麦
<400> 45
atggcggaag ttctggtgag cgcatccaca ggggcaatgg gttccctcct gcggaagttg 60
ggagctatgt tgacggatga atacaagctg ctcaagaacg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgcg ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacaggaac acgggtcctc ctgttccgag 300
gcggctcatg gcgtcgccaa gctcatggac aagtgcaaga acttgctgcc ggacatcaag 360
gcccgccgca ggatcgccaa ggaggtcaaa gacatcaaga aagaaattaa ggatgtcagc 420
gacagatttt caaggtacca gatcttgttc tccttacagt actattagta cctaccatca 480
gatttgtgac cgatttgttg ccactcaagg tacaagattg acgactcctc ctcctctatg 540
ccggcaaaag acaaggtcga ccctcgactt cgtgcagtct acaaagacgc cgcagagctc 600
gtcggaattg atggaccaaa ggatgaactt gtcaagtggc tgaatgagaa agaaggccaa 660
tcactgaaat ctgtctctat tgttggatat ggagggttgg gcaagaccac gctcgccaac 720
cagatccggg tcaaccttgg agcgaccttc gactgcgggg cttttgtctc gatttcacgc 780
aagcctgaca tgaaggcgat attaagatct atattatcac aaatcaccaa gaaagacgat 840
gcttgctcta gattagatga tatacaactc atcatcgaca agattcgaga attcctccaa 900
gacacaaggt atgtatgtga gatctgctac gaagtaacat tgcttaatta gctcatgcat 960
aatcggttgc ctagaatttt gctaatgaag attgctttgt gtgatttact ttcgtcctca 1020
acttgtatga ctgcgcttaa actttcaagt cattgtttct tgcaacttat gtcgatctgt 1080
tcactaatta agatatagct aaaatgtgtt tgccgatttc tgtcgccaca cattttctaa 1140
tttgattgcc acaagtttgt taaacttgcg gctatgttct gtttattaaa taattgtgac 1200
atggcgtcag tcaaaacaat aaagtacatg caattccctc tcttttgtac aagataaata 1260
tccgcacgca ctacatgact gcaaatatat ctatcttcta aggacccaaa gcacttgcat 1320
gtgcagacca ttgttaccgg atatgcagta cattaggttt gtgattagcc actttaagaa 1380
catagggttt ctagaggcta taaacctacg gttatcagta ttataatctt gaatggatat 1440
caaacatccg ttgaagaatc tatgcccata acacaaaaag agaaaaggga agaattaatc 1500
atagtcagat cggaccagcc tgactagcgt aacataatga caacagtagg ttgttgcgtc 1560
tttcctggtt tcgtagcaat ttgcatgacg aattgaggcg cagagccacc actcaatatg 1620
tggtcctacc aatcatgagg aaataacttg ttaccaaaat gacaaaagaa aataggtagt 1680
ccccatcatt gtttcaatat ctatcctctt gcgaacatgc tattggcagt ggcaaaacat 1740
tgtaccttgc cactggcaac agctgccttt actcggcctg agcattcata tttttttcct 1800
cttggttgtt aaatcttgct atttttcgct tgccttctat ggcaatcttt ttttttctta 1860
tattgccacc aaattgtatc taagagcaac tccaacacgc cgacgcaatt tttgtccatt 1920
tagatcggcc aagcggacgg ggtgtctggt tttgcctttg ggtggtgcgc ctacgcatta 1980
gtccgcgtgg gcatttaaaa aacttaaaaa gcttaaaata attaaatata aataaaaatg 2040
catattaaag cccgtcaaag tccacataat taaacataat acattaatat attaataaaa 2100
aacagccgcc ccagcccgca gccctaggcg tcttcgtcgg aggagtgggt gaggtcgaag 2160
aatgccggca gctcagccca cgggaaggcc tgctggtggg cagccagcac cgcctgctcc 2220
gctgtctgcg gcggcggcgg ccctacctgg caccgcgcac gcctcctcct cgcgctcctg 2280
ccgctcctcc tcctcatcgc acgcccaacg aagttttggc gctcctcccc ctcgggttcc 2340
cgctgcatgc ggacctggcc gtcgtaccgc tcctcctcca gcgtgcggtc cttccacgcc 2400
acgaggtagg ccgcctcctc ctccatcacg cacagccaaa tgggcggctt gctcatccgc 2460
ttgcagacga tgccctgcca ttggtaaacg ccggtgggcg cgggtggtgc tagcgctggt 2520
gcaggcggtg acgatgtggc tggtggtccc ggcaggtaga cactatagct ggcggccgat 2580
agctgcagcg tgtcatcgag gccagaccac taggagtcct cgcactaaga ctcctcggtg 2640
acgtgatggg ccccctcctc caatgccttg aggaacgccg cctcctcctc aacctactat 2700
ttgaccaccg gtggcggcgg tggagggcag gcttcgagct ggacggcgtc gcggaggcgc 2760
acatggtgct cgatggtgaa ccagacttct cagttagggg agtctggcct cgatgggtgg 2820
agtcgcgacg ctgctctggc gtcaggagag cacgccgggc ccgaatctcg ttggcgcgcg 2880
cacgctcgga ctgtggcacc gccggcactg gaatgtggtg ggggtcgagg tgccagccgt 2940
gggggaagtt cacgtccggc cacggcagtg agacgcggta ctcccggtgg tactacgcgt 3000
ggtgcaccgg gatgtactgg tggtcccgtg gcgctgccgc cctcccatcc cgcagcgccg 3060
acaccaatgc gaaccccttc gctgccggcc tcaccggccc ttgcaacgag ccggcctcgt 3120
ggtcgtgctt gccttgccct tacctttgcc aaagaaaccc atgacggcta gggttttctg 3180
tcaccggcga gggagcagat gtggactaga gtgggaggag aagtggatga ggcccgcccc 3240
gccgcaccct tgcaattaaa aagggcgtga tccggacgtt tctagtcact gccatgcggg 3300
cccgaggtag gtgggcgcca ttgactacgt ctgtggtggt agttggctgg cagacacgcc 3360
agcccgaggc gcacaccgag cggacgcgca acacgtccgt ctcgtgttcg tgtgaacgca 3420
aatcaggcac aaatttgggc tggaaatgcg tctacatgga tgccgaacgg acgtgatttg 3480
ggtttgtgtc cgcacgttgg gccggcgttt ttgtgccttt cgaccctaat ggacacacac 3540
ggaccaaatc cgttggcgtg ttagagttgc ttgtacctag tactactatg tgtggagttg 3600
ttatctttga gtttctcccc catctgtagc tgtgtattat tgtgagatgc tctcaggatt 3660
tcacttattt tagataatga caactttctt gatgctagac aattacatca aggtgtatta 3720
tatctaggat gcacacagat gtgagattgt ttcaggattt taggttactt ggtaggtgtt 3780
actataaata tctaatgagc tctaatgtgt atgttcatga aactgtgttg caggtacttt 3840
atcataattg atgatatatg ggagttagga acatgggaaa ctttgaaatg tgcatttgtc 3900
aagaatacat tgggcagcag aataattatc acaacacgta tagttgatgt tgccaaatct 3960
tgttctcctt ctagtgaaga tcttgtctat gagatgaaac cactcagtga ggctgactcg 4020
aagaaattgt ttttcaagag gatatttggc tgtgaagaaa gctgtcctga tagcttgaaa 4080
gaagctgcca atgatatttt gaaaaaatgc cgtggcctac ctctggccat caatgccatt 4140
tctagcgtgt tggtcacaac aagggaaaca aaagaagagt gggatcgggt gcgacattca 4200
atacgctctt caaaggtcaa aagtgatata atagagacca tgaattacat attgtctctt 4260
agttattttg atcttcccca ccacctaaga agctgcttat tgtacctggc tctgtttcct 4320
gaagaccaat tgattggaag gaagcggtta gtacgcagat ggatttctga aggctttatc 4380
catggagaaa gtggacaaga tttcatggaa ttaggagagg aatattttca ccagcttgtg 4440
aacagaagtc taatacagcc cggcaacata gggtatgacg gcaaggcaat gtattgccga 4500
gtccatgaca ccatccttga tttcctaatt gacaaatcct ctgaagagaa catgtgtact 4560
gtcctaaaga aacaatgcaa gcctaatggc atagttcgtc ggctctctct aatggggaat 4620
gaggatgaag aaatcgtgga gcaattggat ctgtcacacg ctcggtcaat cactgctttt 4680
ggggatatca agctattgcc ttcgcttggg aggtcaaaat gcttgcgcgt gctagacttg 4740
caagactgcg atcagttgga aaatcatcat atcaaggata ttgaaagact ctatcagcta 4800
agatacttgg atatctcttc tacaggaatc acggagcttc ccaggcaaat tggggagttg 4860
ctgtatctag agacgctagt tgcctatgga ttacgtgagc ttcctgaaag cacaagtcgg 4920
ctacaacgac tggcacgttt gtttgtttac tctggttgta agcttccagg tgggttggga 4980
aacttgataa acttgcaaga gcttgactgc gttgatgcct tgcatttgaa gcatgtggaa 5040
gagctcggca aactaacaaa tctgagaaag ctgagtatta aattggacac tggtgggatt 5100
gaaggcaaca aattagaaga gtccaaggaa aagctggtgt cctctctctg taaactggac 5160
gaatgcggcc tcctttccct gagtattgat tattatctga gagaaaagga tggagaagag 5220
ccgttcctcc ctgctttggg ttgtatccag gaggtttttg tatatggaca agatatcagc 5280
cggattagca gatggctcgc gtcacttccc aacctacaca ggttgctttt ggacgatccg 5340
aagatagagc agcaggatat tgagatgatt ggattgatac ccaatctgat cgatttgaca 5400
ctgccgcctc tatacaaaac agacgacgct gggcggctca tcatcaggcg tgaaggattt 5460
caacagttgc agaagtttga ggcttacaat actcgtatgg gggttttaat gtttgaaccg 5520
ggggctatgc cgaggctcaa ggagcttaaa ctccataact tcatcgaaaa gcccaaatct 5580
gctgcggtcg atttcgactt tggcatccaa cgcctctcca gccttgctcg cctcactgtc 5640
agcttatcct gcggtggctg gacggtggca gaagtagagg ctgcggagga tgctttcaag 5700
agcatggccg aggcgaaccc caaccgtcca atactggaga tgacaagata taatacgcag 5760
catatgttac aagacgagca aataggtatg acaggctcag gtactattaa ctagtttgta 5820
cttttgtctt ggtgagcagt agatcaaaag atgtgcatat gcttcaatct tttttccggt 5880
aatctatttc agctacaacg ccggccgtca agtcctag 5918
<210> 46
<211> 5918
<212> DNA
<213> 普通小麦
<400> 46
atggcggaag ttctggtgag cgcatccaca ggggcaatgg gttccctcct gcggaagttg 60
ggagctatgt tgacggatga atacaagctg ctcaagaacg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgcg ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacaggaac acgggtcctc ctgttccgag 300
gcggctcatg gcgtcgccaa gctcatggac aagtgcaaga acttgctgcc ggacatcaag 360
gcccgccgca ggatcgccaa ggaggtcaaa gacatcaaga aagaaattaa ggatgtcagc 420
gacagatttt caaggtacca gatcttgttc tccttacagt actattagta cctaccatca 480
gatttgtgac cgatttgttg ccactcaagg tacaagattg acgactcctc ctcctctatg 540
ccggcaaaag acaaggtcga ccctcgactt cgtgcagtct acaaagacgc cgcagagctc 600
gtcggaattg atggaccaaa ggatgaactt gtcaagtggc tgaatgagaa agaaggccaa 660
tcactgaaat ctgtctctat tgttggatat ggagggttgg gcaagaccac gctcgccaac 720
cagatccggg tcaaccttgg agcgaccttc gactgcgggg cttttgtctc gatttcacgc 780
aagcctgaca tgaaggcgat attaagatct atattatcac aaatcaccaa gaaagacgat 840
gcttgctcta gattagatga tatacaactc atcatcgaca agattcgaga attcctccaa 900
gacacaaggt atgtatgtga gatctgctac gaagtaacat tgcttaatta gctcatgcat 960
aatcggttgc ctagaatttt gctaatgaag attgctttgt gtgatttact ttcgtcctca 1020
acttgtatga ctgcgcttaa actttcaagt cattgtttct tgcaacttat gtcgatctgt 1080
tcactaatta agatatagct aaaatgtgtt tgccgatttc tgtcgccaca cattttctaa 1140
tttgattgcc acaagtttgt taaacttgcg gctatgttct gtttattaaa taattgtgac 1200
atggcgtcag tcaaaacaat aaagtacatg caattccctc tcttttgtac aagataaata 1260
tccgcacgca ctacatgact gcaaatatat ctatcttcta aggacccaaa gcacttgcat 1320
gtgcagacca ttgttaccgg atatgcagta cattaggttt gtgattagcc actttaagaa 1380
catagggttt ctagaggcta taaacctacg gttatcagta ttataatctt gaatggatat 1440
caaacatccg ttgaagaatc tatgcccata acacaaaaag agaaaaggga agaattaatc 1500
atagtcagat cggaccagcc tgactagcgt aacataatga caacagtagg ttgttgcgtc 1560
tttcctggtt tcgtagcaat ttgcatgacg aattgaggcg cagagccacc actcaatatg 1620
tggtcctacc aatcatgagg aaataacttg ttaccaaaat gacaaaagaa aataggtagt 1680
ccccatcatt gtttcaatat ctatcctctt gcgaacatgc tattggcagt ggcaaaacat 1740
tgtaccttgc cactggcaac agctgccttt actcggcctg agcattcata tttttttcct 1800
cttggttgtt aaatcttgct atttttcgct tgccttctat ggcaatcttt ttttttctta 1860
tattgccacc aaattgtatc taagagcaac tccaacacgc cgacgcaatt tttgtccatt 1920
tagatcggcc aagcggacgg ggtgtctggt tttgcctttg ggtggtgcgc ctacgcatta 1980
gtccgcgtgg gcatttaaaa aacttaaaaa gcttaaaata attaaatata aataaaaatg 2040
catattaaag cccgtcaaag tccacataat taaacataat acattaatat attaataaaa 2100
aacagccgcc ccagcccgca gccctaggcg tcttcgtcgg aggagtgggt gaggtcgaag 2160
aatgccggca gctcagccca cgggaaggcc tgctggtggg cagccagcac cgcctgctcc 2220
gctgtctgcg gcggcggcgg ccctacctgg caccgcgcac gcctcctcct cgcgctcctg 2280
ccgctcctcc tcctcatcgc acgcccaacg aagttttggc gctcctcccc ctcgggttcc 2340
cgctgcatgc ggacctggcc gtcgtaccgc tcctcctcca gcgtgcggtc cttccacgcc 2400
acgaggtagg ccgcctcctc ctccatcacg cacagccaaa tgggcggctt gctcatccgc 2460
ttgcagacga tgccctgcca ttggtaaacg ccggtgggcg cgggtggtgc tagcgctggt 2520
gcaggcggtg acgatgtggc tggtggtccc ggcaggtaga cactatagct ggcggccgat 2580
agctgcagcg tgtcatcgag gccagaccac taggagtcct cgcactaaga ctcctcggtg 2640
acgtgatggg ccccctcctc caatgccttg aggaacgccg cctcctcctc aacctactat 2700
ttgaccaccg gtggcggcgg tggagggcag gcttcgagct ggacggcgtc gcggaggcgc 2760
acatggtgct cgatggtgaa ccagacttct cagttagggg agtctggcct cgatgggtgg 2820
agtcgcgacg ctgctctggc gtcaggagag cacgccgggc ccgaatctcg ttggcgcgcg 2880
cacgctcgga ctgtggcacc gccggcactg gaatgtggtg ggggtcgagg tgccagccgt 2940
gggggaagtt cacgtccggc cacggcagtg agacgcggta ctcccggtgg tactacgcgt 3000
ggtgcaccgg gatgtactgg tggtcccgtg gcgctgccgc cctcccatcc cgcagcgccg 3060
acaccaatgc gaaccccttc gctgccggcc tcaccggccc ttgcaacgag ccggcctcgt 3120
ggtcgtgctt gccttgccct tacctttgcc aaagaaaccc atgacggcta gggttttctg 3180
tcaccggcga gggagcagat gtggactaga gtgggaggag aagtggatga ggcccgcccc 3240
gccgcaccct tgcaattaaa aagggcgtga tccggacgtt tctagtcact gccatgcggg 3300
cccgaggtag gtgggcgcca ttgactacgt ctgtggtggt agttggctgg cagacacgcc 3360
agcccgaggc gcacaccgag cggacgcgca acacgtccgt ctcgtgttcg tgtgaacgca 3420
aatcaggcac aaatttgggc tggaaatgcg tctacatgga tgccgaacgg acgtgatttg 3480
ggtttgtgtc cgcacgttgg gccggcgttt ttgtgccttt cgaccctaat ggacacacac 3540
ggaccaaatc cgttggcgtg ttagagttgc ttgtacctag tactactatg tgtggagttg 3600
ttatctttga gtttctcccc catctgtagc tgtgtattat tgtgagatgc tctcaggatt 3660
tcacttattt tagataatga caactttctt gatgctagac aattacatca aggtgtatta 3720
tatctaggat gcacacagat gtgagattgt ttcaggattt taggttactt ggtaggtgtt 3780
actataaata tctaatgagc tctaatgtgt atgttcatga aactgtgttg caggtacttt 3840
atcataattg atgatatatg ggagttagga acatgggaaa ctttgaaatg tgcatttgtc 3900
aagaatacat tgggcagcag aataattatc acaacacgta tagttgatgt tgccaaatct 3960
tgttctcctt ctagtgaaga tcttgtctat gagatgaaac cactcagtga ggctgactcg 4020
aagaaattgt ttttcaagag gatatttggc tgtgaagaaa gctgtcctga tagcttgaaa 4080
gaagctgcca atgatatttt gaaaaaatgc cgtggcctac ctctggccat caatgccatt 4140
tctagcgtgt tggtcacaac aagggaaaca aaagaagagt gggatcgggt gcgacattca 4200
atacgctctt caaaggtcaa aagtgatata atagagacca tgaattacat attgtctctt 4260
agttattttg atcttcccca ccacctaaga agctgcttat tgtacctggc tctgtttcct 4320
gaagaccaat tgattggaag gaagcggtta gtacgcagat ggatttctga aggctttatc 4380
catggagaaa gtggacaaga tctcatggaa ttaggagagg aatattttca ccagcttgtg 4440
aacagaagtc taatacagcc cggcaacata gggtatgacg gcaaggcaat gtattgccga 4500
gtccatgaca ccatccttga tttcctaatt gacaaatcct ctgaagagaa catgtgtact 4560
gtcctaaaga aacaatgcaa gcctaatggc atagttcgtc ggctctctct aatggggaat 4620
gaggatgaag aaatcgtgga gcaattggat ctgtcacacg ctcggtcaat cactgctttt 4680
ggggatatca agctattgcc ttcgcttggg aggtcaaaat gcttgcgcgt gctagacttg 4740
caaaactgcg atcagttgga aaatcatcat atcaaggata ttgaaagact ctatcagcta 4800
agatacttgg atatctcttc tacaggaatc acggagcttc ccaggcaaat tggggagttg 4860
ctgtatctag agacgctagt tgcctatgga ttacgtgagc ttcctgaaag cacaagtcgg 4920
ctacaacgac tggcacgttt gtttgtttac tctggttgta agcttccagg tgggttggga 4980
aacttgataa acttgcaaga gcttgactgc gttgatgcct tgcatttgaa gcatgtggaa 5040
gagctcggca aactaacaaa tctgagaaag ctgagtatta aattggacac tggtgggatt 5100
gaaggcaaca aattagaaga gtccaaggaa aagctggtgt cctctctctg taaactggac 5160
gaatgcggcc tcctttccct gagtattgat tattatctga gagaaaagga tggagaagag 5220
ccgttcctcc ctgctttggg ttgtatccag gaggtttttg tatatggaca agatatcagc 5280
cggattagca gatggctcgc gtcacttccc aacctacaca ggttgctttt ggacgatccg 5340
aagatagagc agcaggatat tgagatgatt ggattgatac ccaatctgat cgatttgaca 5400
ctgccgcctc tatacaaaac agacgacgct gggcggctca tcatcaggcg tgaaggattt 5460
caacagttgc agaagtttga ggcttacaat actcgtatgg gggttttaat gtttgaaccg 5520
ggggctatgc cgaggctcaa ggagcttaaa ctccataact tcatcgaaaa gcccaaatct 5580
gctgcggtcg atttcgactt tggcatccaa cgcctctcca gccttgctcg cctcactgtc 5640
agcttatcct gcggtggctg gacggtggca gaagtagagg ctgcggagga tgctttcaag 5700
agcatggccg aggcgaaccc caaccgtcca atactggaga tgacaagata taatacgcag 5760
catatgttac aagacgagca aataggtatg acaggctcag gtactattaa ctagtttgta 5820
cttttgtctt ggtgagcagt agatcaaaag atgtgcatat gcttcaatct tttttccggt 5880
aatctatttc agctacaacg ccggccgtca agtcctag 5918
<210> 47
<211> 5918
<212> DNA
<213> 普通小麦
<400> 47
atggcggaag ttctggtgag cgcatccaca ggggcaatgg gttccctcct gcggaagttg 60
ggagctatgt tgacggatga atacaagctg ctcaagaacg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgcg ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacaggaac acgggtcctc ctgttccgag 300
gcggctcatg gcgtcgccaa gctcatggac aagtgcaaga acttgctgcc ggacatcaag 360
gcccgccgca ggatcgccaa ggaggtcaaa gacatcaaga aagaaattaa ggatgtcagc 420
gacagatttt caaggtacca gatcttgttc tccttacagt actattagta cctaccatca 480
gatttgtgac cgatttgttg ccactcaagg tacaagattg acgactcctc ctcctctatg 540
ccggcaaaag acaaggtcga ccctcgactt cgtgcagtct acaaagacgc cgcagagctc 600
gtcggaattg atggaccaaa ggatgaactt gtcaagtggc tgaatgagaa agaaggccaa 660
tcactgaaat ctgtctctat tgttggatat ggagggttgg gcaagaccac gctcgccaac 720
cagatccggg tcaaccttgg agcgaccttc gactgcgggg cttttgtctc gatttcacgc 780
aagcctgaca tgaaggcgat attaagatct atattatcac aaatcaccaa gaaagacgat 840
gcttgctcta gattagatga tatacaactc atcatcgaca agattcgaga attcctccaa 900
gacacaaggt atgtatgtga gatctgctac gaagtaacat tgcttaatta gctcatgcat 960
aatcggttgc ctagaatttt gctaatgaag attgctttgt gtgatttact ttcgtcctca 1020
acttgtatga ctgcgcttaa actttcaagt cattgtttct tgcaacttat gtcgatctgt 1080
tcactaatta agatatagct aaaatgtgtt tgccgatttc tgtcgccaca cattttctaa 1140
tttgattgcc acaagtttgt taaacttgcg gctatgttct gtttattaaa taattgtgac 1200
atggcgtcag tcaaaacaat aaagtacatg caattccctc tcttttgtac aagataaata 1260
tccgcacgca ctacatgact gcaaatatat ctatcttcta aggacccaaa gcacttgcat 1320
gtgcagacca ttgttaccgg atatgcagta cattaggttt gtgattagcc actttaagaa 1380
catagggttt ctagaggcta taaacctacg gttatcagta ttataatctt gaatggatat 1440
caaacatccg ttgaagaatc tatgcccata acacaaaaag agaaaaggga agaattaatc 1500
atagtcagat cggaccagcc tgactagcgt aacataatga caacagtagg ttgttgcgtc 1560
tttcctggtt tcgtagcaat ttgcatgacg aattgaggcg cagagccacc actcaatatg 1620
tggtcctacc aatcatgagg aaataacttg ttaccaaaat gacaaaagaa aataggtagt 1680
ccccatcatt gtttcaatat ctatcctctt gcgaacatgc tattggcagt ggcaaaacat 1740
tgtaccttgc cactggcaac agctgccttt actcggcctg agcattcata tttttttcct 1800
cttggttgtt aaatcttgct atttttcgct tgccttctat ggcaatcttt ttttttctta 1860
tattgccacc aaattgtatc taagagcaac tccaacacgc cgacgcaatt tttgtccatt 1920
tagatcggcc aagcggacgg ggtgtctggt tttgcctttg ggtggtgcgc ctacgcatta 1980
gtccgcgtgg gcatttaaaa aacttaaaaa gcttaaaata attaaatata aataaaaatg 2040
catattaaag cccgtcaaag tccacataat taaacataat acattaatat attaataaaa 2100
aacagccgcc ccagcccgca gccctaggcg tcttcgtcgg aggagtgggt gaggtcgaag 2160
aatgccggca gctcagccca cgggaaggcc tgctggtggg cagccagcac cgcctgctcc 2220
gctgtctgcg gcggcggcgg ccctacctgg caccgcgcac gcctcctcct cgcgctcctg 2280
ccgctcctcc tcctcatcgc acgcccaacg aagttttggc gctcctcccc ctcgggttcc 2340
cgctgcatgc ggacctggcc gtcgtaccgc tcctcctcca gcgtgcggtc cttccacgcc 2400
acgaggtagg ccgcctcctc ctccatcacg cacagccaaa tgggcggctt gctcatccgc 2460
ttgcagacga tgccctgcca ttggtaaacg ccggtgggcg cgggtggtgc tagcgctggt 2520
gcaggcggtg acgatgtggc tggtggtccc ggcaggtaga cactatagct ggcggccgat 2580
agctgcagcg tgtcatcgag gccagaccac taggagtcct cgcactaaga ctcctcggtg 2640
acgtgatggg ccccctcctc caatgccttg aggaacgccg cctcctcctc aacctactat 2700
ttgaccaccg gtggcggcgg tggagggcag gcttcgagct ggacggcgtc gcggaggcgc 2760
acatggtgct cgatggtgaa ccagacttct cagttagggg agtctggcct cgatgggtgg 2820
agtcgcgacg ctgctctggc gtcaggagag cacgccgggc ccgaatctcg ttggcgcgcg 2880
cacgctcgga ctgtggcacc gccggcactg gaatgtggtg ggggtcgagg tgccagccgt 2940
gggggaagtt cacgtccggc cacggcagtg agacgcggta ctcccggtgg tactacgcgt 3000
ggtgcaccgg gatgtactgg tggtcccgtg gcgctgccgc cctcccatcc cgcagcgccg 3060
acaccaatgc gaaccccttc gctgccggcc tcaccggccc ttgcaacgag ccggcctcgt 3120
ggtcgtgctt gccttgccct tacctttgcc aaagaaaccc atgacggcta gggttttctg 3180
tcaccggcga gggagcagat gtggactaga gtgggaggag aagtggatga ggcccgcccc 3240
gccgcaccct tgcaattaaa aagggcgtga tccggacgtt tctagtcact gccatgcggg 3300
cccgaggtag gtgggcgcca ttgactacgt ctgtggtggt agttggctgg cagacacgcc 3360
agcccgaggc gcacaccgag cggacgcgca acacgtccgt ctcgtgttcg tgtgaacgca 3420
aatcaggcac aaatttgggc tggaaatgcg tctacatgga tgccgaacgg acgtgatttg 3480
ggtttgtgtc cgcacgttgg gccggcgttt ttgtgccttt cgaccctaat ggacacacac 3540
ggaccaaatc cgttggcgtg ttagagttgc ttgtacctag tactactatg tgtggagttg 3600
ttatctttga gtttctcccc catctgtagc tgtgtattat tgtgagatgc tctcaggatt 3660
tcacttattt tagataatga caactttctt gatgctagac aattacatca aggtgtatta 3720
tatctaggat gcacacagat gtgagattgt ttcaggattt taggttactt ggtaggtgtt 3780
actataaata tctaatgagc tctaatgtgt atgttcatga aactgtgttg caggtacttt 3840
atcataattg atgatatatg ggagttagga acatgggaaa ctttgaaatg tgcatttgtc 3900
aagaatacat tgggcagcag aataattatc acaacacgta tagttgatgt tgccaaatct 3960
tgttctcctt ctagtgaaga tcttgtctat gagatgaaac cactcagtga ggctgactcg 4020
aagaaattgt ttttcaagag gatatttggc tgtgaagaaa gctgtcctga tagcttgaaa 4080
gaagctgcca atgatatttt gaaaaaatgc cgtggcctac ctctggccat caatgccatt 4140
tctagcgtgt tggtcacaac aagggaaaca aaagaagagt gggatcgggt gcgacattca 4200
atacgctctt caaaggtcaa aagtgatata atagagacca tgaattacat attgtctctt 4260
agttattttg atcttcccca ccacctaaga agctgcttat tgtacctggc tctgtttcct 4320
gaagaccaat tgattggaag gaagcggtta gtacgcagat ggatttctga aggctttatc 4380
catggagaaa gtggacaaga tctcatggaa ttaggagagg aatattttca ccagcttgtg 4440
aacagaagtc taatacagcc cggcaacata gggtatgacg gcaaggcaat gtattgccga 4500
gtccatgaca ccatccttga tttcctaatt gacaaatcct ctgaagagaa catgtgtact 4560
gtcctaaaga aacaatgcaa gcctaatggc atagttcgtc ggctctctct aatggggaat 4620
gaggatgaag aaatcgtgga gcaattggat ctgtcacacg ctcggtcaat cactgctttt 4680
ggggatatca agctattgcc ttcgcttggg aggtcaaaat gcttgcgcgt gctagacttg 4740
caagactgcg atcagttgga aaatcatcat atcaaggata ttgaaagact ctatcagcta 4800
agatacttgg atatctcttc tacaggaatc acggagcttc ccaggcaaat tgaggagttg 4860
ctgtatctag agacgctagt tgcctatgga ttacgtgagc ttcctgaaag cacaagtcgg 4920
ctacaacgac tggcacgttt gtttgtttac tctggttgta agcttccagg tgggttggga 4980
aacttgataa acttgcaaga gcttgactgc gttgatgcct tgcatttgaa gcatgtggaa 5040
gagctcggca aactaacaaa tctgagaaag ctgagtatta aattggacac tggtgggatt 5100
gaaggcaaca aattagaaga gtccaaggaa aagctggtgt cctctctctg taaactggac 5160
gaatgcggcc tcctttccct gagtattgat tattatctga gagaaaagga tggagaagag 5220
ccgttcctcc ctgctttggg ttgtatccag gaggtttttg tatatggaca agatatcagc 5280
cggattagca gatggctcgc gtcacttccc aacctacaca ggttgctttt ggacgatccg 5340
aagatagagc agcaggatat tgagatgatt ggattgatac ccaatctgat cgatttgaca 5400
ctgccgcctc tatacaaaac agacgacgct gggcggctca tcatcaggcg tgaaggattt 5460
caacagttgc agaagtttga ggcttacaat actcgtatgg gggttttaat gtttgaaccg 5520
ggggctatgc cgaggctcaa ggagcttaaa ctccataact tcatcgaaaa gcccaaatct 5580
gctgcggtcg atttcgactt tggcatccaa cgcctctcca gccttgctcg cctcactgtc 5640
agcttatcct gcggtggctg gacggtggca gaagtagagg ctgcggagga tgctttcaag 5700
agcatggccg aggcgaaccc caaccgtcca atactggaga tgacaagata taatacgcag 5760
catatgttac aagacgagca aataggtatg acaggctcag gtactattaa ctagtttgta 5820
cttttgtctt ggtgagcagt agatcaaaag atgtgcatat gcttcaatct tttttccggt 5880
aatctatttc agctacaacg ccggccgtca agtcctag 5918
<210> 48
<211> 5918
<212> DNA
<213> 普通小麦
<400> 48
atggcggaag ttctggtgag cgcatccaca ggggcaatgg gttccctcct gcggaagttg 60
ggagctatgt tgacggatga atacaagctg ctcaagaacg tccgtgggga catcaagttc 120
ctcaaggacg agctggaggt catgtgcgcg ttcctcctca agatgtcaga tgtggaggag 180
cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag 240
gacaacatcg acaagttcat ggtcctcgtg gaacaggaac acgggtcctc ctgttccgag 300
gcggctcatg gcgtcgccaa gctcatggac aagtgcaaga acttgctgcc ggacatcaag 360
gcccgccgca ggatcgccaa ggaggtcaaa gacatcaaga aagaaattaa ggatgtcagc 420
gacagatttt caaggtacca gatcttgttc tccttacagt actattagta cctaccatca 480
gatttgtgac cgatttgttg ccactcaagg tacaagattg acgactcctc ctcctctatg 540
ccggcaaaag acaaggtcga ccctcgactt cgtgcagtct acaaagacgc cgcagagctc 600
gtcggaattg atggaccaaa ggatgaactt gtcaagtggc tgaatgagaa agaaggccaa 660
tcactgaaat ctgtctctat tgttggatat ggagggttgg gcaagaccac gctcgccaac 720
cagatccggg tcaaccttgg agcgaccttc gactgcgggg cttttgtctc gatttcacgc 780
aagcctgaca tgaaggcgat attaagatct atattatcac aaatcaccaa gaaagacgat 840
gcttgctcta gattagatga tatacaactc atcatcgaca agattcgaga attcctccaa 900
gacacaaggt atgtatgtga gatctgctac gaagtaacat tgcttaatta gctcatgcat 960
aatcggttgc ctagaatttt gctaatgaag attgctttgt gtgatttact ttcgtcctca 1020
acttgtatga ctgcgcttaa actttcaagt cattgtttct tgcaacttat gtcgatctgt 1080
tcactaatta agatatagct aaaatgtgtt tgccgatttc tgtcgccaca cattttctaa 1140
tttgattgcc acaagtttgt taaacttgcg gctatgttct gtttattaaa taattgtgac 1200
atggcgtcag tcaaaacaat aaagtacatg caattccctc tcttttgtac aagataaata 1260
tccgcacgca ctacatgact gcaaatatat ctatcttcta aggacccaaa gcacttgcat 1320
gtgcagacca ttgttaccgg atatgcagta cattaggttt gtgattagcc actttaagaa 1380
catagggttt ctagaggcta taaacctacg gttatcagta ttataatctt gaatggatat 1440
caaacatccg ttgaagaatc tatgcccata acacaaaaag agaaaaggga agaattaatc 1500
atagtcagat cggaccagcc tgactagcgt aacataatga caacagtagg ttgttgcgtc 1560
tttcctggtt tcgtagcaat ttgcatgacg aattgaggcg cagagccacc actcaatatg 1620
tggtcctacc aatcatgagg aaataacttg ttaccaaaat gacaaaagaa aataggtagt 1680
ccccatcatt gtttcaatat ctatcctctt gcgaacatgc tattggcagt ggcaaaacat 1740
tgtaccttgc cactggcaac agctgccttt actcggcctg agcattcata tttttttcct 1800
cttggttgtt aaatcttgct atttttcgct tgccttctat ggcaatcttt ttttttctta 1860
tattgccacc aaattgtatc taagagcaac tccaacacgc cgacgcaatt tttgtccatt 1920
tagatcggcc aagcggacgg ggtgtctggt tttgcctttg ggtggtgcgc ctacgcatta 1980
gtccgcgtgg gcatttaaaa aacttaaaaa gcttaaaata attaaatata aataaaaatg 2040
catattaaag cccgtcaaag tccacataat taaacataat acattaatat attaataaaa 2100
aacagccgcc ccagcccgca gccctaggcg tcttcgtcgg aggagtgggt gaggtcgaag 2160
aatgccggca gctcagccca cgggaaggcc tgctggtggg cagccagcac cgcctgctcc 2220
gctgtctgcg gcggcggcgg ccctacctgg caccgcgcac gcctcctcct cgcgctcctg 2280
ccgctcctcc tcctcatcgc acgcccaacg aagttttggc gctcctcccc ctcgggttcc 2340
cgctgcatgc ggacctggcc gtcgtaccgc tcctcctcca gcgtgcggtc cttccacgcc 2400
acgaggtagg ccgcctcctc ctccatcacg cacagccaaa tgggcggctt gctcatccgc 2460
ttgcagacga tgccctgcca ttggtaaacg ccggtgggcg cgggtggtgc tagcgctggt 2520
gcaggcggtg acgatgtggc tggtggtccc ggcaggtaga cactatagct ggcggccgat 2580
agctgcagcg tgtcatcgag gccagaccac taggagtcct cgcactaaga ctcctcggtg 2640
acgtgatggg ccccctcctc caatgccttg aggaacgccg cctcctcctc aacctactat 2700
ttgaccaccg gtggcggcgg tggagggcag gcttcgagct ggacggcgtc gcggaggcgc 2760
acatggtgct cgatggtgaa ccagacttct cagttagggg agtctggcct cgatgggtgg 2820
agtcgcgacg ctgctctggc gtcaggagag cacgccgggc ccgaatctcg ttggcgcgcg 2880
cacgctcgga ctgtggcacc gccggcactg gaatgtggtg ggggtcgagg tgccagccgt 2940
gggggaagtt cacgtccggc cacggcagtg agacgcggta ctcccggtgg tactacgcgt 3000
ggtgcaccgg gatgtactgg tggtcccgtg gcgctgccgc cctcccatcc cgcagcgccg 3060
acaccaatgc gaaccccttc gctgccggcc tcaccggccc ttgcaacgag ccggcctcgt 3120
ggtcgtgctt gccttgccct tacctttgcc aaagaaaccc atgacggcta gggttttctg 3180
tcaccggcga gggagcagat gtggactaga gtgggaggag aagtggatga ggcccgcccc 3240
gccgcaccct tgcaattaaa aagggcgtga tccggacgtt tctagtcact gccatgcggg 3300
cccgaggtag gtgggcgcca ttgactacgt ctgtggtggt agttggctgg cagacacgcc 3360
agcccgaggc gcacaccgag cggacgcgca acacgtccgt ctcgtgttcg tgtgaacgca 3420
aatcaggcac aaatttgggc tggaaatgcg tctacatgga tgccgaacgg acgtgatttg 3480
ggtttgtgtc cgcacgttgg gccggcgttt ttgtgccttt cgaccctaat ggacacacac 3540
ggaccaaatc cgttggcgtg ttagagttgc ttgtacctag tactactatg tgtggagttg 3600
ttatctttga gtttctcccc catctgtagc tgtgtattat tgtgagatgc tctcaggatt 3660
tcacttattt tagataatga caactttctt gatgctagac aattacatca aggtgtatta 3720
tatctaggat gcacacagat gtgagattgt ttcaggattt taggttactt ggtaggtgtt 3780
actataaata tctaatgagc tctaatgtgt atgttcatga aactgtgttg caggtacttt 3840
atcataattg atgatatatg ggagttagga acatgggaaa ctttgaaatg tgcatttgtc 3900
aagaatacat tgggcagcag aataattatc acaacacgta tagttgatgt tgccaaatct 3960
tgttctcctt ctagtgaaga tcttgtctat gagatgaaac cactcagtga ggctgactcg 4020
aagaaattgt ttttcaagag gatatttggc tgtgaagaaa gctgtcctga tagcttgaaa 4080
gaagctgcca atgatatttt gaaaaaatgc cgtggcctac ctctggccat caatgccatt 4140
tctagcgtgt tggtcacaac aagggaaaca aaagaagagt gggatcgggt gcgacattca 4200
atacgctctt caaaggtcaa aagtgatata atagagacca tgaattacat attgtctctt 4260
agttattttg atcttcccca ccacctaaga agctgcttat tgtacctggc tctgtttcct 4320
gaagaccaat tgattggaag gaagcggtta gtacgcagat ggatttctga aggctttatc 4380
catggagaaa gtggacaaga tctcatggaa ttaggagagg aatattttca ccagcttgtg 4440
aacagaagtc taatacagcc cggcaacata gggtatgacg gcaaggcaat gtattgccga 4500
gtccatgaca ccatccttga tttcctaatt gacaaatcct ctgaagagaa catgtgtact 4560
gtcctaaaga aacaatgcaa gcctaatggc atagttcgtc ggctctctct aatggggaat 4620
gaggatgaag aaatcgtgga gcaattggat ctgtcacacg ctcggtcaat cactgctttt 4680
ggggatatca agctattgcc ttcgcttggg aggtcaaaat gcttgcgcgt gctagacttg 4740
caagactgcg atcagttgga aaatcatcat atcaaggata ttgaaagact ctatcagcta 4800
agatacttgg atatctcttc tacaggaatc acggagcttc ccaggcaaat tggggagttg 4860
ctgtatctag agacgctagt tgcctatgga ttacgtgagc ttcctgaaag cacaagtcgg 4920
ctacaacgac tggcacgttt gtttgtttac tctggttgta agcttccagg tgggttggga 4980
aacttgataa acttgcaaga gcttgactgc gttgatgcct tgcatttgaa gcatgtggaa 5040
gagctcggca aactaacaaa tctgagaaag ctgagtatta aattggacac tggtgggatt 5100
gaaggcaaca aattagaaga gtccaaggaa aagctggtgt cctctctctg taaactggac 5160
gaatgcggcc tcctttccct gagtattgat tattatctga gagaaaagga tggagaagag 5220
ccgttcctcc ctgctttggg ttgtatccag gaggtttttg tatatggaca agatatcagc 5280
cggattagca gatggctcgc gtcacttccc aacctacaca ggttgctttt ggacgatccg 5340
aagatagagc agtaggatat tgagatgatt ggattgatac ccaatctgat cgatttgaca 5400
ctgccgcctc tatacaaaac agacgacgct gggcggctca tcatcaggcg tgaaggattt 5460
caacagttgc agaagtttga ggcttacaat actcgtatgg gggttttaat gtttgaaccg 5520
ggggctatgc cgaggctcaa ggagcttaaa ctccataact tcatcgaaaa gcccaaatct 5580
gctgcggtcg atttcgactt tggcatccaa cgcctctcca gccttgctcg cctcactgtc 5640
agcttatcct gcggtggctg gacggtggca gaagtagagg ctgcggagga tgctttcaag 5700
agcatggccg aggcgaaccc caaccgtcca atactggaga tgacaagata taatacgcag 5760
catatgttac aagacgagca aataggtatg acaggctcag gtactattaa ctagtttgta 5820
cttttgtctt ggtgagcagt agatcaaaag atgtgcatat gcttcaatct tttttccggt 5880
aatctatttc agctacaacg ccggccgtca agtcctag 5918
<210> 49
<211> 6564
<212> DNA
<213> 节节麦
<400> 49
aatgatgggc aggcgacgcg agtgtgttta agaaaaaaaa aagcctcatc aaatatgagt 60
tttttagggg atctgggaat atgagtactg taataattga ttccgtcgcg cctctgcgtc 120
caccgcccaa cagcccaaat cgccgccgcc tcgctcttcc ctactccggc caccgccgcc 180
gccgacggcc acgctccgct gtttcccccc accccacccc tccagcacca tgcctctgtt 240
cctcgttgcc acatcggtaa cctgccccac aggcggcccc accgtgctgc tgtggggtgg 300
ccgtcgatgt cgccggcagg gtcctcccgc gtgcttctcc aagcgacctc tgccagctcc 360
tcccttgtct cgcgtcgcct gccatgcgtg gctcggaccc aaaatctttt cgcccgttcg 420
cggagccggg tagggagggg ggcggagatg ctctgaactt gaagccgaga tatttggagt 480
gcagcagatg gactcatttt tctaagagcg cacatgctat atatcagcgg taaatttcct 540
catacattgt tgctaacgga gcaagatttg tagccaaacg aagatttgtt ttcctggtaa 600
cgaaggattc tgtattgttt cacctaccca taacaaaaag ataacgcatt tctgtatggc 660
agggctgcgt ggtggtcgtt gaagtcgccg gccccgctgt cccatttttt tccacacgcc 720
atcgtccact tgctctgaat ctgcctaact ccagaccatt tcttccacac gttgctctta 780
tccttttgcg cgtgcttccg cgcggtctca tcctgttgac ctgctaaatc tgcacaacta 840
catgccattt ctcttctgca tagatctcac gctctcatcc aggtttcctg gatctgcaca 900
gctgcaggcc atttctcttc tgcatagcgc ttgtagtgtt tccctgtgtt tgcagtgtga 960
gagctaaaat ggcggagttt gtagtcaggc cactggtatc cacgttgatg aacacggcat 1020
ccagctacct gctggaccag tacaaggtga tggatggcat gaaggagcag cgcgagaccc 1080
tcaagcgcca gctgccagcc atcttggaca tcatccaaga tgcggagaag aaaggagctt 1140
ccctacctgg agtgcgcgct tggctcgaag cactcaagaa ggtggcctat gaggcgaacg 1200
atgtctttga tgagttcaag tacgaggccc tccggcgtga cgccaagaag aagggacact 1260
acaagaagct tggctttgac atcgtaagcc tcttccctgc tcacaacccc attgtatttc 1320
gttacaggat gggcaagaag ttgtgcagga ttgtgcgcaa aattgagggc cttgtcagag 1380
agatgaatga cttcggattc aaccaaactc agcaagcacc accatccaag cagtggcgca 1440
acacagattc cataataatt gactctgaga aggatattgt cagcaggtcc agaaacgaag 1500
agaagaagaa gatcgttgat atattgattg atcaagctgg cgatagggac ctcatcgtcc 1560
ttcccattgt tggaatgggc gggctgggca agaccacctt tgctcagctt gtctacaatg 1620
accctataat caaggagcat ttcaagctcc agaggtggtg ttgtgtgtca gatgattttg 1680
atgttgtgaa gattgcaaac aacatctgtg agaccaatga gatccatcgt gaaaaggcat 1740
tgcagaacct tcagaaagaa gtaagtggga agagatacct cattgtgttg gatgatgtat 1800
ggaatgaaga tgctgataag tgggaaaaac tgaaaacctg cctgaagcac ggtgggaagg 1860
ggagtgcaat attgacgacc actcgcaatg tgcaagtagc acgaattatg aagatgtgta 1920
ttgctgatag ccataatctc agaaatctag ataaagtatt tctgaaggaa atatttgaga 1980
atagagcatt ctgtttgcaa aagccaaagg ctgctgagct aagcgatgtg gttgataaga 2040
ttatggacag atgtggaggc tctcctttag ctgccaaagc ctttgggtct atgttgagta 2100
acaagactag catgaaagaa tggacagaca tactagccag aagcaacact tgcaatgagg 2160
gcacaaaaac ttttcttgta ctcaagctca gctatgatga cttgccatca cacttgaaac 2220
aatgctttgc tttttgtgct gtatttccca aagattatga gattggtgtt gaaacattaa 2280
ttcagctatg gatggcacat gatttcatac cactgaaaga aggtgataat cttgagaagg 2340
taggtagaga aatttttgat gagttaactt ggaggtcatt ctttcaagat gtcaagcgaa 2400
tccctcgaag agagtggtgg ggggagctcc gtcctagaac aatatgcaag atacatgatc 2460
ttatgcatga cattgccttg tctgttatgg gaaaagattg tcttactata gttgataggc 2520
ctaatgaaaa ggagttactg tccactggtc ctacacgtta cttgttctca tcatatgagt 2580
atatcggaac tctgttggat gattatctga agaaacactc tcccgctctc cagacactgc 2640
tgtatccgta tccctacacc agtgactcag caccacactt gtcgaagtgc aattatctgc 2700
gagcactgca actcttcagt ttgagaaaac ttccactctg gccaaggcac ttacagcacc 2760
tgaggtatct tgatctctca aataacatgt tgatcgaaga gcttcccaag gaaataagca 2820
tactgtataa tctacagact cttaaccttt gtaactgcag aaggcttgat caacttccag 2880
aggatatgaa gtatatggaa aatctccgtc acctctatac aaatggatgc tcatcattga 2940
agtgcatgcc tccaggcctc gggcaactca cttctctaca gactctaaca tattttgtgg 3000
tgagctctag tcctggttgc agtactatta gagaactaca ggacttgaat cttggtggtg 3060
agttagagct atctcgtctt cagtttgcaa ctgaagtaga tgctaaagca tgcagccttg 3120
gaaataaaga gaaacttaca catttatctc ttaaatgggg tgatgacagc agtgatgaat 3180
tgggccacca caggaatgtg ctcgatgctc ttaaacctca tgctgtcctg gagtttctaa 3240
gaatacgctc ctatagaggt actggcttcc ccgcatgggt ggtaagtatt aattttctgc 3300
agcatttgac cgagctccag ctagatggtt gtacaatgtg tgaggaattt ccccaattcg 3360
gtcaatttaa gtcccttgaa gttcttgttt tgaaaaggtt gaacaaactt caaagcctat 3420
gcaatcacag ttcatctgca atatttccag cattaaaagt tctcagatta aaaaaattgg 3480
agatttttga gagatgggtg gcaacagaag gagaagagtt agcatttcct caacttgaga 3540
atgttaaaat taaggactgc ccaaagctag caattctgcc tgaagcacca aaactcaagt 3600
ttatagctct aaaagaagaa aaggcacagc tatccttatc aatatttaag tccaggtata 3660
tggcttgctt gtctggggtt ggcctgtccg tccgtgatac agaagcagca ccaagaacag 3720
agcttgatca ggattgtgaa gtatctcttt cgaacctgct gttggatggt tgcaactttt 3780
tgttctgctc aactccattg cagccaactg ttggggtctg gaaatggttt ggtcagcttg 3840
tgcatttgga aataaaatct tgtgatatgc tcatctactg gcctgaagaa gagttccgtt 3900
gcttggtatc attgaatagt ttatccatta attcctgcag caagctagta ggacacaccc 3960
aggggaaagg atgccgtacc cgtactcaag taagggatca actcctgcca aatcttaaaa 4020
atttaagaat acatcattgt ggaagtttga cagagctttt tgttctgccg ccatctctca 4080
caagtattga tatgctggat tgcaatagta ttgagtccat actggggcag gatgatacag 4140
agttggagag tatactacac tttgatacag catcatcatc ggaacacttc aatgacctca 4200
cgtccacatg tttgctagag cagtcacttt cacccagaat taatcctttg ccatgtcttg 4260
attatttgcg tatagttagc tgtaagaagc ttcgtttcgt gccagtgcag ttagatgcac 4320
tccaattttt gtgtatttct gattgcaatg ggctggagtc gctggattgt ttgggagacc 4380
taccgtcact ggaatatcta taccttatgg gctgcaaaca tctggcatct gtaccaggaa 4440
gtcttgggag ttactcggcc cttcagaagc tccgcattga atactgccca gctttaaata 4500
tgaagccact tcatggacat ctccaacagc ggcttgatag ccttgaattg aaggatctat 4560
ctaaggctgg ttcaagccat cctaatgaag gtacctttca atttcattta ccccattttg 4620
tatctctttt tccatctgta tagtttacag atgtacatat tgcagcctgt agtttcaagt 4680
ttatccaact ccttgtattt tcacatttgg tatgtagtga ccgcgtacac agtttctctg 4740
atatggctat tccaatatca tttatttgta taccgtaagt ttgacttgga ttgacgtcgt 4800
catgtgtggc cgatctgtat tttctatcat tttggtaaaa gttaatacac catggaagta 4860
tgctctcttg tgtagtgacc gtacacagat cattctttta tgtctatccc actatcattt 4920
atttgtaaag tttgacttgc tattaatgtt cacagagata ttctgacatt gttttccatg 4980
tacagggcct aaactatggg aaccaaaatc ctggaaatat atgattcctt cactgcgcaa 5040
gcgtgagtct gaatagagat acaactacca tgtatgtttc tggttttttt ttcttgcaac 5100
tttgattcta aaagtttgta ggctaaaaga tcggttttgg tatgttcacc attcttgacc 5160
tgaatgtgtt taatttcctt gcagggtcgc tcgtgtgcgc atacaagtga tgaacacaat 5220
tggtaactgc cctcttcaag ggagtcaagt tcttttattt gcctgctccg tgagctctga 5280
tgaaaataag gcgctcgaag gcctgaagca tgggggaaaa tgaacttcaa cttgcagaac 5340
tggagagaga aggtcgtgct gaatcgtgtt tactttgtgc agaacaaaca agcatgtcct 5400
tgggcaaatc gtgcggacta taagtgcact tgcagtcgtg tgctggcgat cgagcctttt 5460
gttttccatg tgtacttaca gttcaccgta gtaatacggt gtgctatttc acatctttta 5520
cataatcttt agagcagagc acgatcctgg ttaattcaga cagctgatta gcagacatct 5580
ggttagttca gagttagaga ttgtgtgcgt gtgtttatgg ctgcttcaga gagctgattg 5640
tgtgcgtgtt tttgtgcgtg cacgacgtgc tagtatctgc ttgccaattc gccatgtaca 5700
caaaagtctg caaccaaact tggaagatct caaattacca gtgcctttca ggatgaacat 5760
acagtacatg atttcagaag agtagagtgc aggtgccagc ttccaccatt ttgttctgcc 5820
gcaaaccaga gctgatcgca gcaaagaaat tatatagaca gccatggtag tatagtaagc 5880
tcactctagt tcttcagttc cagcaggcac acatgtcctc tcaacagaac agaacagaag 5940
ggaacagccc cacaccgcag gctgttgtca ccgactcacc gtgcacaaac ctgcttccgt 6000
ccattttacg acggtccgtc gcttgccatc tctagcttcc cttttttttg atagaaagac 6060
cgtaatggaa ccccctacag tataattggt gcaacaaatc caaattgcaa gtaccatacc 6120
ttgaatctgg gtgggtggga aggcatcaac ccctccccat cactaggcta tgccttagtc 6180
tgccatctct agcttcccta ccgaccaggg aatcacggct cgtgctaccc cttttagcac 6240
cccgacgagg agtagcttct gcacggatta tagtggggaa gaaaatgtat gactaggata 6300
caggaattcc agagaagcca aattcaggaa acaaccaatc cctttatttt acaaagccgc 6360
ccataaacct ttttttaata aaacatggta gtacggggat tttattttaa taaacaacca 6420
atccccttct tttacctggt attatgattt tatccattcc ccaataacca acataatttc 6480
ttctaccaaa aaaacaaccc ttggtgatga atttattcag cataatgaga agaacatgta 6540
taactgcaat gcagcaggaa tttc 6564
<210> 50
<211> 1230
<212> PRT
<213> 节节麦
<220>
<221> 结构域
<222> (32)..(55)
<223> 卷曲螺旋结构域
<220>
<221> 结构域
<222> (175)..(452)
<223> 核苷酸结合结构域
<220>
<221> 结构域
<222> (578)..(867)
<223> 富含亮氨酸重复结构域
<400> 50
Met Ala Glu Phe Val Val Arg Pro Leu Val Ser Thr Leu Met Asn Thr
1 5 10 15
Ala Ser Ser Tyr Leu Leu Asp Gln Tyr Lys Val Met Asp Gly Met Lys
20 25 30
Glu Gln Arg Glu Thr Leu Lys Arg Gln Leu Pro Ala Ile Leu Asp Ile
35 40 45
Ile Gln Asp Ala Glu Lys Lys Gly Ala Ser Leu Pro Gly Val Arg Ala
50 55 60
Trp Leu Glu Ala Leu Lys Lys Val Ala Tyr Glu Ala Asn Asp Val Phe
65 70 75 80
Asp Glu Phe Lys Tyr Glu Ala Leu Arg Arg Asp Ala Lys Lys Lys Gly
85 90 95
His Tyr Lys Lys Leu Gly Phe Asp Ile Val Ser Leu Phe Pro Ala His
100 105 110
Asn Pro Ile Val Phe Arg Tyr Arg Met Gly Lys Lys Leu Cys Arg Ile
115 120 125
Val Arg Lys Ile Glu Gly Leu Val Arg Glu Met Asn Asp Phe Gly Phe
130 135 140
Asn Gln Thr Gln Gln Ala Pro Pro Ser Lys Gln Trp Arg Asn Thr Asp
145 150 155 160
Ser Ile Ile Ile Asp Ser Glu Lys Asp Ile Val Ser Arg Ser Arg Asn
165 170 175
Glu Glu Lys Lys Lys Ile Val Asp Ile Leu Ile Asp Gln Ala Gly Asp
180 185 190
Arg Asp Leu Ile Val Leu Pro Ile Val Gly Met Gly Gly Leu Gly Lys
195 200 205
Thr Thr Phe Ala Gln Leu Val Tyr Asn Asp Pro Ile Ile Lys Glu His
210 215 220
Phe Lys Leu Gln Arg Trp Cys Cys Val Ser Asp Asp Phe Asp Val Val
225 230 235 240
Lys Ile Ala Asn Asn Ile Cys Glu Thr Asn Glu Ile His Arg Glu Lys
245 250 255
Ala Leu Gln Asn Leu Gln Lys Glu Val Ser Gly Lys Arg Tyr Leu Ile
260 265 270
Val Leu Asp Asp Val Trp Asn Glu Asp Ala Asp Lys Trp Glu Lys Leu
275 280 285
Lys Thr Cys Leu Lys His Gly Gly Lys Gly Ser Ala Ile Leu Thr Thr
290 295 300
Thr Arg Asn Val Gln Val Ala Arg Ile Met Lys Met Cys Ile Ala Asp
305 310 315 320
Ser His Asn Leu Arg Asn Leu Asp Lys Val Phe Leu Lys Glu Ile Phe
325 330 335
Glu Asn Arg Ala Phe Cys Leu Gln Lys Pro Lys Ala Ala Glu Leu Ser
340 345 350
Asp Val Val Asp Lys Ile Met Asp Arg Cys Gly Gly Ser Pro Leu Ala
355 360 365
Ala Lys Ala Phe Gly Ser Met Leu Ser Asn Lys Thr Ser Met Lys Glu
370 375 380
Trp Thr Asp Ile Leu Ala Arg Ser Asn Thr Cys Asn Glu Gly Thr Lys
385 390 395 400
Thr Phe Leu Val Leu Lys Leu Ser Tyr Asp Asp Leu Pro Ser His Leu
405 410 415
Lys Gln Cys Phe Ala Phe Cys Ala Val Phe Pro Lys Asp Tyr Glu Ile
420 425 430
Gly Val Glu Thr Leu Ile Gln Leu Trp Met Ala His Asp Phe Ile Pro
435 440 445
Leu Lys Glu Gly Asp Asn Leu Glu Lys Val Gly Arg Glu Ile Phe Asp
450 455 460
Glu Leu Thr Trp Arg Ser Phe Phe Gln Asp Val Lys Arg Ile Pro Arg
465 470 475 480
Arg Glu Trp Trp Gly Glu Leu Arg Pro Arg Thr Ile Cys Lys Ile His
485 490 495
Asp Leu Met His Asp Ile Ala Leu Ser Val Met Gly Lys Asp Cys Leu
500 505 510
Thr Ile Val Asp Arg Pro Asn Glu Lys Glu Leu Leu Ser Thr Gly Pro
515 520 525
Thr Arg Tyr Leu Phe Ser Ser Tyr Glu Tyr Ile Gly Thr Leu Leu Asp
530 535 540
Asp Tyr Leu Lys Lys His Ser Pro Ala Leu Gln Thr Leu Leu Tyr Pro
545 550 555 560
Tyr Pro Tyr Thr Ser Asp Ser Ala Pro His Leu Ser Lys Cys Asn Tyr
565 570 575
Leu Arg Ala Leu Gln Leu Phe Ser Leu Arg Lys Leu Pro Leu Trp Pro
580 585 590
Arg His Leu Gln His Leu Arg Tyr Leu Asp Leu Ser Asn Asn Met Leu
595 600 605
Ile Glu Glu Leu Pro Lys Glu Ile Ser Ile Leu Tyr Asn Leu Gln Thr
610 615 620
Leu Asn Leu Cys Asn Cys Arg Arg Leu Asp Gln Leu Pro Glu Asp Met
625 630 635 640
Lys Tyr Met Glu Asn Leu Arg His Leu Tyr Thr Asn Gly Cys Ser Ser
645 650 655
Leu Lys Cys Met Pro Pro Gly Leu Gly Gln Leu Thr Ser Leu Gln Thr
660 665 670
Leu Thr Tyr Phe Val Val Ser Ser Ser Pro Gly Cys Ser Thr Ile Arg
675 680 685
Glu Leu Gln Asp Leu Asn Leu Gly Gly Glu Leu Glu Leu Ser Arg Leu
690 695 700
Gln Phe Ala Thr Glu Val Asp Ala Lys Ala Cys Ser Leu Gly Asn Lys
705 710 715 720
Glu Lys Leu Thr His Leu Ser Leu Lys Trp Gly Asp Asp Ser Ser Asp
725 730 735
Glu Leu Gly His His Arg Asn Val Leu Asp Ala Leu Lys Pro His Ala
740 745 750
Val Leu Glu Phe Leu Arg Ile Arg Ser Tyr Arg Gly Thr Gly Phe Pro
755 760 765
Ala Trp Val Val Ser Ile Asn Phe Leu Gln His Leu Thr Glu Leu Gln
770 775 780
Leu Asp Gly Cys Thr Met Cys Glu Glu Phe Pro Gln Phe Gly Gln Phe
785 790 795 800
Lys Ser Leu Glu Val Leu Val Leu Lys Arg Leu Asn Lys Leu Gln Ser
805 810 815
Leu Cys Asn His Ser Ser Ser Ala Ile Phe Pro Ala Leu Lys Val Leu
820 825 830
Arg Leu Lys Lys Leu Glu Ile Phe Glu Arg Trp Val Ala Thr Glu Gly
835 840 845
Glu Glu Leu Ala Phe Pro Gln Leu Glu Asn Val Lys Ile Lys Asp Cys
850 855 860
Pro Lys Leu Ala Ile Leu Pro Glu Ala Pro Lys Leu Lys Phe Ile Ala
865 870 875 880
Leu Lys Glu Glu Lys Ala Gln Leu Ser Leu Ser Ile Phe Lys Ser Arg
885 890 895
Tyr Met Ala Cys Leu Ser Gly Val Gly Leu Ser Val Arg Asp Thr Glu
900 905 910
Ala Ala Pro Arg Thr Glu Leu Asp Gln Asp Cys Glu Val Ser Leu Ser
915 920 925
Asn Leu Leu Leu Asp Gly Cys Asn Phe Leu Phe Cys Ser Thr Pro Leu
930 935 940
Gln Pro Thr Val Gly Val Trp Lys Trp Phe Gly Gln Leu Val His Leu
945 950 955 960
Glu Ile Lys Ser Cys Asp Met Leu Ile Tyr Trp Pro Glu Glu Glu Phe
965 970 975
Arg Cys Leu Val Ser Leu Asn Ser Leu Ser Ile Asn Ser Cys Ser Lys
980 985 990
Leu Val Gly His Thr Gln Gly Lys Gly Cys Arg Thr Arg Thr Gln Val
995 1000 1005
Arg Asp Gln Leu Leu Pro Asn Leu Lys Asn Leu Arg Ile His His Cys
1010 1015 1020
Gly Ser Leu Thr Glu Leu Phe Val Leu Pro Pro Ser Leu Thr Ser Ile
1025 1030 1035 1040
Asp Met Leu Asp Cys Asn Ser Ile Glu Ser Ile Leu Gly Gln Asp Asp
1045 1050 1055
Thr Glu Leu Glu Ser Ile Leu His Phe Asp Thr Ala Ser Ser Ser Glu
1060 1065 1070
His Phe Asn Asp Leu Thr Ser Thr Cys Leu Leu Glu Gln Ser Leu Ser
1075 1080 1085
Pro Arg Ile Asn Pro Leu Pro Cys Leu Asp Tyr Leu Arg Ile Val Ser
1090 1095 1100
Cys Lys Lys Leu Arg Phe Val Pro Val Gln Leu Asp Ala Leu Gln Phe
1105 1110 1115 1120
Leu Cys Ile Ser Asp Cys Asn Gly Leu Glu Ser Leu Asp Cys Leu Gly
1125 1130 1135
Asp Leu Pro Ser Leu Glu Tyr Leu Tyr Leu Met Gly Cys Lys His Leu
1140 1145 1150
Ala Ser Val Pro Gly Ser Leu Gly Ser Tyr Ser Ala Leu Gln Lys Leu
1155 1160 1165
Arg Ile Glu Tyr Cys Pro Ala Leu Asn Met Lys Pro Leu His Gly His
1170 1175 1180
Leu Gln Gln Arg Leu Asp Ser Leu Glu Leu Lys Asp Leu Ser Lys Ala
1185 1190 1195 1200
Gly Ser Ser His Pro Asn Glu Gly Pro Lys Leu Trp Glu Pro Lys Ser
1205 1210 1215
Trp Lys Tyr Met Ile Pro Ser Leu Arg Lys Arg Glu Ser Glu
1220 1225 1230
<210> 51
<211> 3690
<212> DNA
<213> 节节麦
<400> 51
atggcggagt ttgtagtcag gccactggta tccacgttga tgaacacggc atccagctac 60
ctgctggacc agtacaaggt gatggatggc atgaaggagc agcgcgagac cctcaagcgc 120
cagctgccag ccatcttgga catcatccaa gatgcggaga agaaaggagc ttccctacct 180
ggagtgcgcg cttggctcga agcactcaag aaggtggcct atgaggcgaa cgatgtcttt 240
gatgagttca agtacgaggc cctccggcgt gacgccaaga agaagggaca ctacaagaag 300
cttggctttg acatcgtaag cctcttccct gctcacaacc ccattgtatt tcgttacagg 360
atgggcaaga agttgtgcag gattgtgcgc aaaattgagg gccttgtcag agagatgaat 420
gacttcggat tcaaccaaac tcagcaagca ccaccatcca agcagtggcg caacacagat 480
tccataataa ttgactctga gaaggatatt gtcagcaggt ccagaaacga agagaagaag 540
aagatcgttg atatattgat tgatcaagct ggcgataggg acctcatcgt ccttcccatt 600
gttggaatgg gcgggctggg caagaccacc tttgctcagc ttgtctacaa tgaccctata 660
atcaaggagc atttcaagct ccagaggtgg tgttgtgtgt cagatgattt tgatgttgtg 720
aagattgcaa acaacatctg tgagaccaat gagatccatc gtgaaaaggc attgcagaac 780
cttcagaaag aagtaagtgg gaagagatac ctcattgtgt tggatgatgt atggaatgaa 840
gatgctgata agtgggaaaa actgaaaacc tgcctgaagc acggtgggaa ggggagtgca 900
atattgacga ccactcgcaa tgtgcaagta gcacgaatta tgaagatgtg tattgctgat 960
agccataatc tcagaaatct agataaagta tttctgaagg aaatatttga gaatagagca 1020
ttctgtttgc aaaagccaaa ggctgctgag ctaagcgatg tggttgataa gattatggac 1080
agatgtggag gctctccttt agctgccaaa gcctttgggt ctatgttgag taacaagact 1140
agcatgaaag aatggacaga catactagcc agaagcaaca cttgcaatga gggcacaaaa 1200
acttttcttg tactcaagct cagctatgat gacttgccat cacacttgaa acaatgcttt 1260
gctttttgtg ctgtatttcc caaagattat gagattggtg ttgaaacatt aattcagcta 1320
tggatggcac atgatttcat accactgaaa gaaggtgata atcttgagaa ggtaggtaga 1380
gaaatttttg atgagttaac ttggaggtca ttctttcaag atgtcaagcg aatccctcga 1440
agagagtggt ggggggagct ccgtcctaga acaatatgca agatacatga tcttatgcat 1500
gacattgcct tgtctgttat gggaaaagat tgtcttacta tagttgatag gcctaatgaa 1560
aaggagttac tgtccactgg tcctacacgt tacttgttct catcatatga gtatatcgga 1620
actctgttgg atgattatct gaagaaacac tctcccgctc tccagacact gctgtatccg 1680
tatccctaca ccagtgactc agcaccacac ttgtcgaagt gcaattatct gcgagcactg 1740
caactcttca gtttgagaaa acttccactc tggccaaggc acttacagca cctgaggtat 1800
cttgatctct caaataacat gttgatcgaa gagcttccca aggaaataag catactgtat 1860
aatctacaga ctcttaacct ttgtaactgc agaaggcttg atcaacttcc agaggatatg 1920
aagtatatgg aaaatctccg tcacctctat acaaatggat gctcatcatt gaagtgcatg 1980
cctccaggcc tcgggcaact cacttctcta cagactctaa catattttgt ggtgagctct 2040
agtcctggtt gcagtactat tagagaacta caggacttga atcttggtgg tgagttagag 2100
ctatctcgtc ttcagtttgc aactgaagta gatgctaaag catgcagcct tggaaataaa 2160
gagaaactta cacatttatc tcttaaatgg ggtgatgaca gcagtgatga attgggccac 2220
cacaggaatg tgctcgatgc tcttaaacct catgctgtcc tggagtttct aagaatacgc 2280
tcctatagag gtactggctt ccccgcatgg gtggtaagta ttaattttct gcagcatttg 2340
accgagctcc agctagatgg ttgtacaatg tgtgaggaat ttccccaatt cggtcaattt 2400
aagtcccttg aagttcttgt tttgaaaagg ttgaacaaac ttcaaagcct atgcaatcac 2460
agttcatctg caatatttcc agcattaaaa gttctcagat taaaaaaatt ggagattttt 2520
gagagatggg tggcaacaga aggagaagag ttagcatttc ctcaacttga gaatgttaaa 2580
attaaggact gcccaaagct agcaattctg cctgaagcac caaaactcaa gtttatagct 2640
ctaaaagaag aaaaggcaca gctatcctta tcaatattta agtccaggta tatggcttgc 2700
ttgtctgggg ttggcctgtc cgtccgtgat acagaagcag caccaagaac agagcttgat 2760
caggattgtg aagtatctct ttcgaacctg ctgttggatg gttgcaactt tttgttctgc 2820
tcaactccat tgcagccaac tgttggggtc tggaaatggt ttggtcagct tgtgcatttg 2880
gaaataaaat cttgtgatat gctcatctac tggcctgaag aagagttccg ttgcttggta 2940
tcattgaata gtttatccat taattcctgc agcaagctag taggacacac ccaggggaaa 3000
ggatgccgta cccgtactca agtaagggat caactcctgc caaatcttaa aaatttaaga 3060
atacatcatt gtggaagttt gacagagctt tttgttctgc cgccatctct cacaagtatt 3120
gatatgctgg attgcaatag tattgagtcc atactggggc aggatgatac agagttggag 3180
agtatactac actttgatac agcatcatca tcggaacact tcaatgacct cacgtccaca 3240
tgtttgctag agcagtcact ttcacccaga attaatcctt tgccatgtct tgattatttg 3300
cgtatagtta gctgtaagaa gcttcgtttc gtgccagtgc agttagatgc actccaattt 3360
ttgtgtattt ctgattgcaa tgggctggag tcgctggatt gtttgggaga cctaccgtca 3420
ctggaatatc tataccttat gggctgcaaa catctggcat ctgtaccagg aagtcttggg 3480
agttactcgg cccttcagaa gctccgcatt gaatactgcc cagctttaaa tatgaagcca 3540
cttcatggac atctccaaca gcggcttgat agccttgaat tgaaggatct atctaaggct 3600
ggttcaagcc atcctaatga agggcctaaa ctatgggaac caaaatcctg gaaatatatg 3660
attccttcac tgcgcaagcg tgagtctgaa 3690
<210> 52
<211> 4088
<212> DNA
<213> 普通小麦
<400> 52
atggcggagt ttgtagtcag gccactggta tccacgttga tgaacacggc atccagctac 60
ctgctggacc agtacaaggt gatggatggc atgaaggagc agcgcgagac cctcaagcgc 120
cagctgccag ccatcttgga catcatccaa gatgcggaga agaaaggagc ttccctacct 180
ggagtgcgcg cttggctcga agcactcaag aaggtggcct atgaggcgaa cgatgtcttt 240
gatgagttca agtacgaggc cctccggcgt gacgccaaga agaagggaca ctacaagaag 300
cttggctttg acatcgtaag cctcttccct gctcacaacc ccattgtatt tcgttacagg 360
atgggcaaga agttgtgcag gattgtgcgc aaaattgagg gccttgtcag agagatgaat 420
gacttcggat tcaaccaaac tcagcaagca ccaccatcca agcagtgacg caacacagat 480
tccataataa ttgactctga gaaggatatt gtcagcaggt ccagaaacga agagaagaag 540
aagatcgttg atatattgat tgatcaagct ggcgataggg acctcatcgt ccttcccatt 600
gttggaatgg gcgggctggg caagaccacc tttgctcagc ttgtctacaa tgaccctata 660
atcaaggagc atttcaagct ccagaggtgg tgttgtgtgt cagatgattt tgatgttgtg 720
aagattgcaa acaacatctg tgagaccaat gagatccatc gtgaaaaggc attgcagaac 780
cttcagaaag aagtaagtgg gaagagatac ctcattgtgt tggatgatgt atggaatgaa 840
gatgctgata agtgggaaaa actgaaaacc tgcctgaagc acggtgggaa ggggagtgca 900
atattgacga ccactcgcaa tgtgcaagta gcacgaatta tgaagatgtg tattgctgat 960
agccataatc tcagaaatct agataaagta tttctgaagg aaatatttga gaatagagca 1020
ttctgtttgc aaaagccaaa ggctgctgag ctaagcgatg tggttgataa gattatggac 1080
agatgtggag gctctccttt agctgccaaa gcctttgggt ctatgttgag taacaagact 1140
agcatgaaag aatggacaga catactagcc agaagcaaca cttgcaatga gggcacaaaa 1200
acttttcttg tactcaagct cagctatgat gacttgccat cacacttgaa acaatgcttt 1260
gctttttgtg ctgtatttcc caaagattat gagattggtg ttgaaacatt aattcagcta 1320
tggatggcac atgatttcat accactgaaa gaaggtgata atcttgagaa ggtaggtaga 1380
gaaatttttg atgagttaac ttggaggtca ttctttcaag atgtcaagcg aatccctcga 1440
agagagtggt ggggggagct ccgtcctaga acaatatgca agatacatga tcttatgcat 1500
gacattgcct tgtctgttat gggaaaagat tgtcttacta tagttgatag gcctaatgaa 1560
aaggagttac tgtccactgg tcctacacgt tacttgttct catcatatga gtatatcgga 1620
actctgttgg atgattatct gaagaaacac tctcccgctc tccagacact gctgtatccg 1680
tatccctaca ccagtgactc agcaccacac ttgtcgaagt gcaattatct gcgagcactg 1740
caactcttca gtttgagaaa acttccactc tggccaaggc acttacagca cctgaggtat 1800
cttgatctct caaataacat gttgatcgaa gagcttccca aggaaataag catactgtat 1860
aatctacaga ctcttaacct ttgtaactgc agaaggcttg atcaacttcc agaggatatg 1920
aagtatatgg aaaatctccg tcacctctat acaaatggat gctcatcatt gaagtgcatg 1980
cctccaggcc tcgggcaact cacttctcta cagactctaa catattttgt ggtgagctct 2040
agtcctggtt gcagtactat tagagaacta caggacttga atcttggtgg tgagttagag 2100
ctatctcgtc ttcagtttgc aactgaagta gatgctaaag catgcagcct tggaaataaa 2160
gagaaactta cacatttatc tcttaaatgg ggtgatgaca gcagtgatga attgggccac 2220
cacaggaatg tgctcgatgc tcttaaacct catgctgtcc tggagtttct aagaatacgc 2280
tcctatagag gtactggctt ccccgcatgg gtggtaagta ttaattttct gcagcatttg 2340
accgagctcc agctagatgg ttgtacaatg tgtgaggaat ttccccaatt cggtcaattt 2400
aagtcccttg aagttcttgt tttgaaaagg ttgaacaaac ttcaaagcct atgcaatcac 2460
agttcatctg caatatttcc agcattaaaa gttctcagat taaaaaaatt ggagattttt 2520
gagagatggg tggcaacaga aggagaagag ttagcatttc ctcaacttga gaatgttaaa 2580
attaaggact gcccaaagct agcaattctg cctgaagcac caaaactcaa gtttatagct 2640
ctaaaagaag aaaaggcaca gctatcctta tcaatattta agtccaggta tatggcttgc 2700
ttgtctgggg ttggcctgtc cgtccgtgat acagaagcag caccaagaac agagcttgat 2760
caggattgtg aagtatctct ttcgaacctg ctgttggatg gttgcaactt tttgttctgc 2820
tcaactccat tgcagccaac tgttggggtc tggaaatggt ttggtcagct tgtgcatttg 2880
gaaataaaat cttgtgatat gctcatctac tggcctgaag aagagttccg ttgcttggta 2940
tcattgaata gtttatccat taattcctgc agcaagctag taggacacac ccaggggaaa 3000
ggatgccgta cccgtactca agtaagggat caactcctgc caaatcttaa aaatttaaga 3060
atacatcatt gtggaagttt gacagagctt tttgttctgc cgccatctct cacaagtatt 3120
gatatgctgg attgcaatag tattgagtcc atactggggc aggatgatac agagttggag 3180
agtatactac actttgatac agcatcatca tcggaacact tcaatgacct cacgtccaca 3240
tgtttgctag agcagtcact ttcacccaga attaatcctt tgccatgtct tgattatttg 3300
cgtatagtta gctgtaagaa gcttcgtttc gtgccagtgc agttagatgc actccaattt 3360
ttgtgtattt ctgattgcaa tgggctggag tcgctggatt gtttgggaga cctaccgtca 3420
ctggaatatc tataccttat gggctgcaaa catctggcat ctgtaccagg aagtcttggg 3480
agttactcgg cccttcagaa gctccgcatt gaatactgcc cagctttaaa tatgaagcca 3540
cttcatggac atctccaaca gcggcttgat agccttgaat tgaaggatct atctaaggct 3600
ggttcaagcc atcctaatga aggtaccttt caatttcatt taccccattt tgtatctctt 3660
tttccatctg tatagtttac agatgtacat attgcagcct gtagtttcaa gtttatccaa 3720
ctccttgtat tttcacattt ggtatgtagt gaccgcgtac acagtttctc tgatatggct 3780
attccaatat catttatttg tataccgtaa gtttgacttg gattgacgtc gtcatgtgtg 3840
gccgatctgt attttctatc attttggtaa aagttaatac accatggaag tatgctctct 3900
tgtgtagtga ccgtacacag atcattcttt tatgtctatc ccactatcat ttatttgtaa 3960
agtttgactt gctattaatg ttcacagaga tattctgaca ttgttttcca tgtacagggc 4020
ctaaactatg ggaaccaaaa tcctggaaat atatgattcc ttcactgcgc aagcgtgagt 4080
ctgaatag 4088
<210> 53
<211> 4088
<212> DNA
<213> 普通小麦
<400> 53
atggcggagt ttgtagtcag gccactggta tccacgttga tgaacacggc atccagctac 60
ctgctggacc agtacaaggt gatggatggc atgaaggagc agcgcgagac cctcaagcgc 120
cagctgccag ccatcttgga catcatccaa gatgcggaga agaaaggagc ttccctacct 180
ggagtgcgcg cttggctcga agcactcaag aaggtggcct atgaggcgaa cgatgtcttt 240
gatgagttca agtacgaggc cctccggcgt gacgccaaga agaagggaca ctacaagaag 300
cttggctttg acatcgtaag cctcttccct gctcacaacc ccattgtatt tcgttacagg 360
atgggcaaga agttgtgcag gattgtgcgc aaaattgagg gccttgtcag agagatgaat 420
gacttcggat tcaaccaaac tcagcaagca ccaccatcca agcagtggcg caacacagat 480
tccataataa ttgactctga gaaggatatt gtcagcaggt tcagaaacga agagaagaag 540
aagatcgttg atatattgat tgatcaagct ggcgataggg acctcatcgt ccttcccatt 600
gttggaatgg gcgggctggg caagaccacc tttgctcagc ttgtctacaa tgaccctata 660
atcaaggagc atttcaagct ccagaggtgg tgttgtgtgt cagatgattt tgatgttgtg 720
aagattgcaa acaacatctg tgagaccaat gagatccatc gtgaaaaggc attgcagaac 780
cttcagaaag aagtaagtgg gaagagatac ctcattgtgt tggatgatgt atggaatgaa 840
gatgctgata agtgggaaaa actgaaaacc tgcctgaagc acggtgggaa ggggagtgca 900
atattgacga ccactcgcaa tgtgcaagta gcacgaatta tgaagatgtg tattgctgat 960
agccataatc tcagaaatct agataaagta tttctgaagg aaatatttga gaatagagca 1020
ttctgtttgc aaaagccaaa ggctgctgag ctaagcgatg tggttgataa gattatggac 1080
agatgtggag gctctccttt agctgccaaa gcctttgggt ctatgttgag taacaagact 1140
agcatgaaag aatggacaga catactagcc agaagcaaca cttgcaatga gggcacaaaa 1200
acttttcttg tactcaagct cagctatgat gacttgccat cacacttgaa acaatgcttt 1260
gctttttgtg ctgtatttcc caaagattat gagattggtg ttgaaacatt aattcagcta 1320
tggatggcac atgatttcat accactgaaa gaaggtgata atcttgagaa ggtaggtaga 1380
gaaatttttg atgagttaac ttggaggtca ttctttcaag atgtcaagcg aatccctcga 1440
agagagtggt ggggggagct ccgtcctaga acaatatgca agatacatga tcttatgcat 1500
gacattgcct tgtctgttat gggaaaagat tgtcttacta tagttgatag gcctaatgaa 1560
aaggagttac tgtccactgg tcctacacgt tacttgttct catcatatga gtatatcgga 1620
actctgttgg atgattatct gaagaaacac tctcccgctc tccagacact gctgtatccg 1680
tatccctaca ccagtgactc agcaccacac ttgtcgaagt gcaattatct gcgagcactg 1740
caactcttca gtttgagaaa acttccactc tggccaaggc acttacagca cctgaggtat 1800
cttgatctct caaataacat gttgatcgaa gagcttccca aggaaataag catactgtat 1860
aatctacaga ctcttaacct ttgtaactgc agaaggcttg atcaacttcc agaggatatg 1920
aagtatatgg aaaatctccg tcacctctat acaaatggat gctcatcatt gaagtgcatg 1980
cctccaggcc tcgggcaact cacttctcta cagactctaa catattttgt ggtgagctct 2040
agtcctggtt gcagtactat tagagaacta caggacttga atcttggtgg tgagttagag 2100
ctatctcgtc ttcagtttgc aactgaagta gatgctaaag catgcagcct tggaaataaa 2160
gagaaactta cacatttatc tcttaaatgg ggtgatgaca gcagtgatga attgggccac 2220
cacaggaatg tgctcgatgc tcttaaacct catgctgtcc tggagtttct aagaatacgc 2280
tcctatagag gtactggctt ccccgcatgg gtggtaagta ttaattttct gcagcatttg 2340
accgagctcc agctagatgg ttgtacaatg tgtgaggaat ttccccaatt cggtcaattt 2400
aagtcccttg aagttcttgt tttgaaaagg ttgaacaaac ttcaaagcct atgcaatcac 2460
agttcatctg caatatttcc agcattaaaa gttctcagat taaaaaaatt ggagattttt 2520
gagagatggg tggcaacaga aggagaagag ttagcatttc ctcaacttga gaatgttaaa 2580
attaaggact gcccaaagct agcaattctg cctgaagcac caaaactcaa gtttatagct 2640
ctaaaagaag aaaaggcaca gctatcctta tcaatattta agtccaggta tatggcttgc 2700
ttgtctgggg ttggcctgtc cgtccgtgat acagaagcag caccaagaac agagcttgat 2760
caggattgtg aagtatctct ttcgaacctg ctgttggatg gttgcaactt tttgttctgc 2820
tcaactccat tgcagccaac tgttggggtc tggaaatggt ttggtcagct tgtgcatttg 2880
gaaataaaat cttgtgatat gctcatctac tggcctgaag aagagttccg ttgcttggta 2940
tcattgaata gtttatccat taattcctgc agcaagctag taggacacac ccaggggaaa 3000
ggatgccgta cccgtactca agtaagggat caactcctgc caaatcttaa aaatttaaga 3060
atacatcatt gtggaagttt gacagagctt tttgttctgc cgccatctct cacaagtatt 3120
gatatgctgg attgcaatag tattgagtcc atactggggc aggatgatac agagttggag 3180
agtatactac actttgatac agcatcatca tcggaacact tcaatgacct cacgtccaca 3240
tgtttgctag agcagtcact ttcacccaga attaatcctt tgccatgtct tgattatttg 3300
cgtatagtta gctgtaagaa gcttcgtttc gtgccagtgc agttagatgc actccaattt 3360
ttgtgtattt ctgattgcaa tgggctggag tcgctggatt gtttgggaga cctaccgtca 3420
ctggaatatc tataccttat gggctgcaaa catctggcat ctgtaccagg aagtcttggg 3480
agttactcgg cccttcagaa gctccgcatt gaatactgcc cagctttaaa tatgaagcca 3540
cttcatggac atctccaaca gcggcttgat agccttgaat tgaaggatct atctaaggct 3600
ggttcaagcc atcctaatga aggtaccttt caatttcatt taccccattt tgtatctctt 3660
tttccatctg tatagtttac agatgtacat attgcagcct gtagtttcaa gtttatccaa 3720
ctccttgtat tttcacattt ggtatgtagt gaccgcgtac acagtttctc tgatatggct 3780
attccaatat catttatttg tataccgtaa gtttgacttg gattgacgtc gtcatgtgtg 3840
gccgatctgt attttctatc attttggtaa aagttaatac accatggaag tatgctctct 3900
tgtgtagtga ccgtacacag atcattcttt tatgtctatc ccactatcat ttatttgtaa 3960
agtttgactt gctattaatg ttcacagaga tattctgaca ttgttttcca tgtacagggc 4020
ctaaactatg ggaaccaaaa tcctggaaat atatgattcc ttcactgcgc aagcgtgagt 4080
ctgaatag 4088
<210> 54
<211> 4088
<212> DNA
<213> 普通小麦
<400> 54
atggcggagt ttgtagtcag gccactggta tccacgttga tgaacacggc atccagctac 60
ctgctggacc agtacaaggt gatggatggc atgaaggagc agcgcgagac cctcaagcgc 120
cagctgccag ccatcttgga catcatccaa gatgcggaga agaaaggagc ttccctacct 180
ggagtgcgcg cttggctcga agcactcaag aaggtggcct atgaggcgaa cgatgtcttt 240
gatgagttca agtacgaggc cctccggcgt gacgccaaga agaagggaca ctacaagaag 300
cttggctttg acatcgtaag cctcttccct gctcacaacc ccattgtatt tcgttacagg 360
atgggcaaga agttgtgcag gattgtgcgc aaaattgagg gccttgtcag agagatgaat 420
gacttcggat tcaaccaaac tcagcaagca ccaccatcca agcagtggcg caacacagat 480
tccataataa ttgactctga gaaggatatt gtcagcaggt ccagaaacga agagaagaag 540
aagatcgttg atatattgat tgatcaagct ggcgataggg acctcatcgt ccttcccatt 600
gttggaatgg gcgggctgag caagaccacc tttgctcagc ttgtctacaa tgaccctata 660
atcaaggagc atttcaagct ccagaggtgg tgttgtgtgt cagatgattt tgatgttgtg 720
aagattgcaa acaacatctg tgagaccaat gagatccatc gtgaaaaggc attgcagaac 780
cttcagaaag aagtaagtgg gaagagatac ctcattgtgt tggatgatgt atggaatgaa 840
gatgctgata agtgggaaaa actgaaaacc tgcctgaagc acggtgggaa ggggagtgca 900
atattgacga ccactcgcaa tgtgcaagta gcacgaatta tgaagatgtg tattgctgat 960
agccataatc tcagaaatct agataaagta tttctgaagg aaatatttga gaatagagca 1020
ttctgtttgc aaaagccaaa ggctgctgag ctaagcgatg tggttgataa gattatggac 1080
agatgtggag gctctccttt agctgccaaa gcctttgggt ctatgttgag taacaagact 1140
agcatgaaag aatggacaga catactagcc agaagcaaca cttgcaatga gggcacaaaa 1200
acttttcttg tactcaagct cagctatgat gacttgccat cacacttgaa acaatgcttt 1260
gctttttgtg ctgtatttcc caaagattat gagattggtg ttgaaacatt aattcagcta 1320
tggatggcac atgatttcat accactgaaa gaaggtgata atcttgagaa ggtaggtaga 1380
gaaatttttg atgagttaac ttggaggtca ttctttcaag atgtcaagcg aatccctcga 1440
agagagtggt ggggggagct ccgtcctaga acaatatgca agatacatga tcttatgcat 1500
gacattgcct tgtctgttat gggaaaagat tgtcttacta tagttgatag gcctaatgaa 1560
aaggagttac tgtccactgg tcctacacgt tacttgttct catcatatga gtatatcgga 1620
actctgttgg atgattatct gaagaaacac tctcccgctc tccagacact gctgtatccg 1680
tatccctaca ccagtgactc agcaccacac ttgtcgaagt gcaattatct gcgagcactg 1740
caactcttca gtttgagaaa acttccactc tggccaaggc acttacagca cctgaggtat 1800
cttgatctct caaataacat gttgatcgaa gagcttccca aggaaataag catactgtat 1860
aatctacaga ctcttaacct ttgtaactgc agaaggcttg atcaacttcc agaggatatg 1920
aagtatatgg aaaatctccg tcacctctat acaaatggat gctcatcatt gaagtgcatg 1980
cctccaggcc tcgggcaact cacttctcta cagactctaa catattttgt ggtgagctct 2040
agtcctggtt gcagtactat tagagaacta caggacttga atcttggtgg tgagttagag 2100
ctatctcgtc ttcagtttgc aactgaagta gatgctaaag catgcagcct tggaaataaa 2160
gagaaactta cacatttatc tcttaaatgg ggtgatgaca gcagtgatga attgggccac 2220
cacaggaatg tgctcgatgc tcttaaacct catgctgtcc tggagtttct aagaatacgc 2280
tcctatagag gtactggctt ccccgcatgg gtggtaagta ttaattttct gcagcatttg 2340
accgagctcc agctagatgg ttgtacaatg tgtgaggaat ttccccaatt cggtcaattt 2400
aagtcccttg aagttcttgt tttgaaaagg ttgaacaaac ttcaaagcct atgcaatcac 2460
agttcatctg caatatttcc agcattaaaa gttctcagat taaaaaaatt ggagattttt 2520
gagagatggg tggcaacaga aggagaagag ttagcatttc ctcaacttga gaatgttaaa 2580
attaaggact gcccaaagct agcaattctg cctgaagcac caaaactcaa gtttatagct 2640
ctaaaagaag aaaaggcaca gctatcctta tcaatattta agtccaggta tatggcttgc 2700
ttgtctgggg ttggcctgtc cgtccgtgat acagaagcag caccaagaac agagcttgat 2760
caggattgtg aagtatctct ttcgaacctg ctgttggatg gttgcaactt tttgttctgc 2820
tcaactccat tgcagccaac tgttggggtc tggaaatggt ttggtcagct tgtgcatttg 2880
gaaataaaat cttgtgatat gctcatctac tggcctgaag aagagttccg ttgcttggta 2940
tcattgaata gtttatccat taattcctgc agcaagctag taggacacac ccaggggaaa 3000
ggatgccgta cccgtactca agtaagggat caactcctgc caaatcttaa aaatttaaga 3060
atacatcatt gtggaagttt gacagagctt tttgttctgc cgccatctct cacaagtatt 3120
gatatgctgg attgcaatag tattgagtcc atactggggc aggatgatac agagttggag 3180
agtatactac actttgatac agcatcatca tcggaacact tcaatgacct cacgtccaca 3240
tgtttgctag agcagtcact ttcacccaga attaatcctt tgccatgtct tgattatttg 3300
cgtatagtta gctgtaagaa gcttcgtttc gtgccagtgc agttagatgc actccaattt 3360
ttgtgtattt ctgattgcaa tgggctggag tcgctggatt gtttgggaga cctaccgtca 3420
ctggaatatc tataccttat gggctgcaaa catctggcat ctgtaccagg aagtcttggg 3480
agttactcgg cccttcagaa gctccgcatt gaatactgcc cagctttaaa tatgaagcca 3540
cttcatggac atctccaaca gcggcttgat agccttgaat tgaaggatct atctaaggct 3600
ggttcaagcc atcctaatga aggtaccttt caatttcatt taccccattt tgtatctctt 3660
tttccatctg tatagtttac agatgtacat attgcagcct gtagtttcaa gtttatccaa 3720
ctccttgtat tttcacattt ggtatgtagt gaccgcgtac acagtttctc tgatatggct 3780
attccaatat catttatttg tataccgtaa gtttgacttg gattgacgtc gtcatgtgtg 3840
gccgatctgt attttctatc attttggtaa aagttaatac accatggaag tatgctctct 3900
tgtgtagtga ccgtacacag atcattcttt tatgtctatc ccactatcat ttatttgtaa 3960
agtttgactt gctattaatg ttcacagaga tattctgaca ttgttttcca tgtacagggc 4020
ctaaactatg ggaaccaaaa tcctggaaat atatgattcc ttcactgcgc aagcgtgagt 4080
ctgaatag 4088
<210> 55
<211> 4088
<212> DNA
<213> 普通小麦
<400> 55
atggcggagt ttgtagtcag gccactggta tccacgttga tgaacacggc atccagctac 60
ctgctggacc agtacaaggt gatggatggc atgaaggagc agcgcgagac cctcaagcgc 120
cagctgccag ccatcttgga catcatccaa gatgcggaga agaaaggagc ttccctacct 180
ggagtgcgcg cttggctcga agcactcaag aaggtggcct atgaggcgaa cgatgtcttt 240
gatgagttca agtacgaggc cctccggcgt gacgccaaga agaagggaca ctacaagaag 300
cttggctttg acatcgtaag cctcttccct gctcacaacc ccattgtatt tcgttacagg 360
atgggcaaga agttgtgcag gattgtgcgc aaaattgagg gccttgtcag agagatgaat 420
gacttcggat tcaaccaaac tcagcaagca ccaccatcca agcagtggcg caacacagat 480
tccataataa ttgactctga gaaggatatt gtcagcaggt ccagaaacga agagaagaag 540
aagatcgttg atatattgat tgatcaagct ggcgataggg acctcatcgt ccttcccatt 600
gttggaatgg gcgggctggg caagaccacc tttgctcagc ttgtctacaa tgaccctata 660
atcaaggagc atttcaagct ccagaggtgg tgttgtgtgt cagatgattt tgatgttgtg 720
aagattgcaa acaacatctg tgagaccaat gagatccatc gtgaaaaggc attgcagaac 780
cttcagaaag aagtaagtgg gaagagatac ctcattgtgt tggatgatgt atggaatgaa 840
gatgctgata agtgggaaaa actgaaaacc tgcctgaagc acggtgggaa ggggagtgca 900
atattgacga ccactcgcaa tgtgcaagta gcacgaatta tgaagatgtg tattgctgat 960
agccataatc tcagaaatct agataaagta tttctgaagg aaatatttga gaatagagca 1020
ttctgtttgc aaaagccaaa ggctgctgag ctaagcgatg tggttgataa gattatggac 1080
agatgtggag gctctccttt agctgccaaa gcctttgggt ctatgttgag taacaagact 1140
agcatgaaag aatggacaga catactagcc agaagcaaca cttgcaatga gggcacaaaa 1200
acttttcttg tactcaagct cagctatgat gacttgccat cacacttgaa acaatgcttt 1260
gctttttgtg ctgtatttcc caaagattat gagattggtg ttgaaacatt aattcagcta 1320
tggatggcac atgatttcat accactgaaa taaggtgata atcttgagaa ggtaggtaga 1380
gaaatttttg atgagttaac ttggaggtca ttctttcaag atgtcaagcg aatccctcga 1440
agagagtggt ggggggagct ccgtcctaga acaatatgca agatacatga tcttatgcat 1500
gacattgcct tgtctgttat gggaaaagat tgtcttacta tagttgatag gcctaatgaa 1560
aaggagttac tgtccactgg tcctacacgt tacttgttct catcatatga gtatatcgga 1620
actctgttgg atgattatct gaagaaacac tctcccgctc tccagacact gctgtatccg 1680
tatccctaca ccagtgactc agcaccacac ttgtcgaagt gcaattatct gcgagcactg 1740
caactcttca gtttgagaaa acttccactc tggccaaggc acttacagca cctgaggtat 1800
cttgatctct caaataacat gttgatcgaa gagcttccca aggaaataag catactgtat 1860
aatctacaga ctcttaacct ttgtaactgc agaaggcttg atcaacttcc agaggatatg 1920
aagtatatgg aaaatctccg tcacctctat acaaatggat gctcatcatt gaagtgcatg 1980
cctccaggcc tcgggcaact cacttctcta cagactctaa catattttgt ggtgagctct 2040
agtcctggtt gcagtactat tagagaacta caggacttga atcttggtgg tgagttagag 2100
ctatctcgtc ttcagtttgc aactgaagta gatgctaaag catgcagcct tggaaataaa 2160
gagaaactta cacatttatc tcttaaatgg ggtgatgaca gcagtgatga attgggccac 2220
cacaggaatg tgctcgatgc tcttaaacct catgctgtcc tggagtttct aagaatacgc 2280
tcctatagag gtactggctt ccccgcatgg gtggtaagta ttaattttct gcagcatttg 2340
accgagctcc agctagatgg ttgtacaatg tgtgaggaat ttccccaatt cggtcaattt 2400
aagtcccttg aagttcttgt tttgaaaagg ttgaacaaac ttcaaagcct atgcaatcac 2460
agttcatctg caatatttcc agcattaaaa gttctcagat taaaaaaatt ggagattttt 2520
gagagatggg tggcaacaga aggagaagag ttagcatttc ctcaacttga gaatgttaaa 2580
attaaggact gcccaaagct agcaattctg cctgaagcac caaaactcaa gtttatagct 2640
ctaaaagaag aaaaggcaca gctatcctta tcaatattta agtccaggta tatggcttgc 2700
ttgtctgggg ttggcctgtc cgtccgtgat acagaagcag caccaagaac agagcttgat 2760
caggattgtg aagtatctct ttcgaacctg ctgttggatg gttgcaactt tttgttctgc 2820
tcaactccat tgcagccaac tgttggggtc tggaaatggt ttggtcagct tgtgcatttg 2880
gaaataaaat cttgtgatat gctcatctac tggcctgaag aagagttccg ttgcttggta 2940
tcattgaata gtttatccat taattcctgc agcaagctag taggacacac ccaggggaaa 3000
ggatgccgta cccgtactca agtaagggat caactcctgc caaatcttaa aaatttaaga 3060
atacatcatt gtggaagttt gacagagctt tttgttctgc cgccatctct cacaagtatt 3120
gatatgctgg attgcaatag tattgagtcc atactggggc aggatgatac agagttggag 3180
agtatactac actttgatac agcatcatca tcggaacact tcaatgacct cacgtccaca 3240
tgtttgctag agcagtcact ttcacccaga attaatcctt tgccatgtct tgattatttg 3300
cgtatagtta gctgtaagaa gcttcgtttc gtgccagtgc agttagatgc actccaattt 3360
ttgtgtattt ctgattgcaa tgggctggag tcgctggatt gtttgggaga cctaccgtca 3420
ctggaatatc tataccttat gggctgcaaa catctggcat ctgtaccagg aagtcttggg 3480
agttactcgg cccttcagaa gctccgcatt gaatactgcc cagctttaaa tatgaagcca 3540
cttcatggac atctccaaca gcggcttgat agccttgaat tgaaggatct atctaaggct 3600
ggttcaagcc atcctaatga aggtaccttt caatttcatt taccccattt tgtatctctt 3660
tttccatctg tatagtttac agatgtacat attgcagcct gtagtttcaa gtttatccaa 3720
ctccttgtat tttcacattt ggtatgtagt gaccgcgtac acagtttctc tgatatggct 3780
attccaatat catttatttg tataccgtaa gtttgacttg gattgacgtc gtcatgtgtg 3840
gccgatctgt attttctatc attttggtaa aagttaatac accatggaag tatgctctct 3900
tgtgtagtga ccgtacacag atcattcttt tatgtctatc ccactatcat ttatttgtaa 3960
agtttgactt gctattaatg ttcacagaga tattctgaca ttgttttcca tgtacagggc 4020
ctaaactatg ggaaccaaaa tcctggaaat atatgattcc ttcactgcgc aagcgtgagt 4080
ctgaatag 4088
<210> 56
<211> 4088
<212> DNA
<213> 普通小麦
<400> 56
atggcggagt ttgtagtcag gccactggta tccacgttga tgaacacggc atccagctac 60
ctgctggacc agtacaaggt gatggatggc atgaaggagc agcgcgagac cctcaagcgc 120
cagctgccag ccatcttgga catcatccaa gatgcggaga agaaaggagc ttccctacct 180
ggagtgcgcg cttggctcga agcactcaag aaggtggcct atgaggcgaa cgatgtcttt 240
gatgagttca agtacgaggc cctccggcgt gacgccaaga agaagggaca ctacaagaag 300
cttggctttg acatcgtaag cctcttccct gctcacaacc ccattgtatt tcgttacagg 360
atgggcaaga agttgtgcag gattgtgcgc aaaattgagg gccttgtcag agagatgaat 420
gacttcggat tcaaccaaac tcagcaagca ccaccatcca agcagtggcg caacacagat 480
tccataataa ttgactctga gaaggatatt gtcagcaggt ccagaaacga agagaagaag 540
aagatcgttg atatattgat tgatcaagct ggcgataggg acctcatcgt ccttcccatt 600
gttggaatgg gcgggctggg caagaccacc tttgctcagc ttgtctacaa tgaccctata 660
atcaaggagc atttcaagct ccagaggtgg tgttgtgtgt cagatgattt tgatgttgtg 720
aagattgcaa acaacatctg tgagaccaat gagatccatc gtgaaaaggc attgcagaac 780
cttcagaaag aagtaagtgg gaagagatac ctcattgtgt tggatgatgt atggaatgaa 840
gatgctgata agtgggaaaa actgaaaacc tgcctgaagc acggtgggaa ggggagtgca 900
atattgacga ccactcgcaa tgtgcaagta gcacgaatta tgaagatgtg tattgctgat 960
agccataatc tcagaaatct agataaagta tttctgaagg aaatatttga gaatagagca 1020
ttctgtttgc aaaagccaaa ggctgctgag ctaagcgatg tggttgataa gattatggac 1080
agatgtggag gctctccttt agctgccaaa gcctttgggt ctatgttgag taacaagact 1140
agcatgaaag aatggacaga catactagcc agaagcaaca cttgcaatga gggcacaaaa 1200
acttttcttg tactcaagct cagctatgat gacttgccat cacacttgaa acaatgcttt 1260
gctttttgtg ctgtatttcc caaagattat gagattggtg ttgaaacatt aattcagcta 1320
tggatggcac atgatttcat accactgaaa gaaggtgata atcttgagaa ggtaggtaga 1380
gaaatttttg atgagttaac ttggaggtca ttctttcaag atgtcaagcg aatccctcga 1440
agagagtgat ggggggagct ccgtcctaga acaatatgca agatacatga tcttatgcat 1500
gacattgcct tgtctgttat gggaaaagat tgtcttacta tagttgatag gcctaatgaa 1560
aaggagttac tgtccactgg tcctacacgt tacttgttct catcatatga gtatatcgga 1620
actctgttgg atgattatct gaagaaacac tctcccgctc tccagacact gctgtatccg 1680
tatccctaca ccagtgactc agcaccacac ttgtcgaagt gcaattatct gcgagcactg 1740
caactcttca gtttgagaaa acttccactc tggccaaggc acttacagca cctgaggtat 1800
cttgatctct caaataacat gttgatcgaa gagcttccca aggaaataag catactgtat 1860
aatctacaga ctcttaacct ttgtaactgc agaaggcttg atcaacttcc agaggatatg 1920
aagtatatgg aaaatctccg tcacctctat acaaatggat gctcatcatt gaagtgcatg 1980
cctccaggcc tcgggcaact cacttctcta cagactctaa catattttgt ggtgagctct 2040
agtcctggtt gcagtactat tagagaacta caggacttga atcttggtgg tgagttagag 2100
ctatctcgtc ttcagtttgc aactgaagta gatgctaaag catgcagcct tggaaataaa 2160
gagaaactta cacatttatc tcttaaatgg ggtgatgaca gcagtgatga attgggccac 2220
cacaggaatg tgctcgatgc tcttaaacct catgctgtcc tggagtttct aagaatacgc 2280
tcctatagag gtactggctt ccccgcatgg gtggtaagta ttaattttct gcagcatttg 2340
accgagctcc agctagatgg ttgtacaatg tgtgaggaat ttccccaatt cggtcaattt 2400
aagtcccttg aagttcttgt tttgaaaagg ttgaacaaac ttcaaagcct atgcaatcac 2460
agttcatctg caatatttcc agcattaaaa gttctcagat taaaaaaatt ggagattttt 2520
gagagatggg tggcaacaga aggagaagag ttagcatttc ctcaacttga gaatgttaaa 2580
attaaggact gcccaaagct agcaattctg cctgaagcac caaaactcaa gtttatagct 2640
ctaaaagaag aaaaggcaca gctatcctta tcaatattta agtccaggta tatggcttgc 2700
ttgtctgggg ttggcctgtc cgtccgtgat acagaagcag caccaagaac agagcttgat 2760
caggattgtg aagtatctct ttcgaacctg ctgttggatg gttgcaactt tttgttctgc 2820
tcaactccat tgcagccaac tgttggggtc tggaaatggt ttggtcagct tgtgcatttg 2880
gaaataaaat cttgtgatat gctcatctac tggcctgaag aagagttccg ttgcttggta 2940
tcattgaata gtttatccat taattcctgc agcaagctag taggacacac ccaggggaaa 3000
ggatgccgta cccgtactca agtaagggat caactcctgc caaatcttaa aaatttaaga 3060
atacatcatt gtggaagttt gacagagctt tttgttctgc cgccatctct cacaagtatt 3120
gatatgctgg attgcaatag tattgagtcc atactggggc aggatgatac agagttggag 3180
agtatactac actttgatac agcatcatca tcggaacact tcaatgacct cacgtccaca 3240
tgtttgctag agcagtcact ttcacccaga attaatcctt tgccatgtct tgattatttg 3300
cgtatagtta gctgtaagaa gcttcgtttc gtgccagtgc agttagatgc actccaattt 3360
ttgtgtattt ctgattgcaa tgggctggag tcgctggatt gtttgggaga cctaccgtca 3420
ctggaatatc tataccttat gggctgcaaa catctggcat ctgtaccagg aagtcttggg 3480
agttactcgg cccttcagaa gctccgcatt gaatactgcc cagctttaaa tatgaagcca 3540
cttcatggac atctccaaca gcggcttgat agccttgaat tgaaggatct atctaaggct 3600
ggttcaagcc atcctaatga aggtaccttt caatttcatt taccccattt tgtatctctt 3660
tttccatctg tatagtttac agatgtacat attgcagcct gtagtttcaa gtttatccaa 3720
ctccttgtat tttcacattt ggtatgtagt gaccgcgtac acagtttctc tgatatggct 3780
attccaatat catttatttg tataccgtaa gtttgacttg gattgacgtc gtcatgtgtg 3840
gccgatctgt attttctatc attttggtaa aagttaatac accatggaag tatgctctct 3900
tgtgtagtga ccgtacacag atcattcttt tatgtctatc ccactatcat ttatttgtaa 3960
agtttgactt gctattaatg ttcacagaga tattctgaca ttgttttcca tgtacagggc 4020
ctaaactatg ggaaccaaaa tcctggaaat atatgattcc ttcactgcgc aagcgtgagt 4080
ctgaatag 4088
<210> 57
<211> 4088
<212> DNA
<213> 普通小麦
<400> 57
atggcggagt ttgtagtcag gccactggta tccacgttga tgaacacggc atccagctac 60
ctgctggacc agtacaaggt gatggatggc atgaaggagc agcgcgagac cctcaagcgc 120
cagctgccag ccatcttgga catcatccaa gatgcggaga agaaaggagc ttccctacct 180
ggagtgcgcg cttggctcga agcactcaag aaggtggcct atgaggcgaa cgatgtcttt 240
gatgagttca agtacgaggc cctccggcgt gacgccaaga agaagggaca ctacaagaag 300
cttggctttg acatcgtaag cctcttccct gctcacaacc ccattgtatt tcgttacagg 360
atgggcaaga agttgtgcag gattgtgcgc aaaattgagg gccttgtcag agagatgaat 420
gacttcggat tcaaccaaac tcagcaagca ccaccatcca agcagtggcg caacacagat 480
tccataataa ttgactctga gaaggatatt gtcagcaggt ccagaaacga agagaagaag 540
aagatcgttg atatattgat tgatcaagct ggcgataggg acctcatcgt ccttcccatt 600
gttggaatgg gcgggctggg caagaccacc tttgctcagc ttgtctacaa tgaccctata 660
atcaaggagc atttcaagct ccagaggtgg tgttgtgtgt cagatgattt tgatgttgtg 720
aagattgcaa acaacatctg tgagaccaat gagatccatc gtgaaaaggc attgcagaac 780
cttcagaaag aagtaagtgg gaagagatac ctcattgtgt tggatgatgt atggaatgaa 840
gatgctgata agtgggaaaa actgaaaacc tgcctgaagc acggtgggaa ggggagtgca 900
atattgacga ccactcgcaa tgtgcaagta gcacgaatta tgaagatgtg tattgctgat 960
agccataatc tcagaaatct agataaagta tttctgaagg aaatatttga gaatagagca 1020
ttctgtttgc aaaagccaaa ggctgctgag ctaagcgatg tggttgataa gattatggac 1080
agatgtggag gctctccttt agctgccaaa gcctttgggt ctatgttgag taacaagact 1140
agcatgaaag aatggacaga catactagcc agaagcaaca cttgcaatga gggcacaaaa 1200
acttttcttg tactcaagct cagctatgat gacttgccat cacacttgaa acaatgcttt 1260
gctttttgtg ctgtatttcc caaagattat gagattggtg ttgaaacatt aattcagcta 1320
tggatggcac atgatttcat accactgaaa gaaggtgata atcttgagaa ggtaggtaga 1380
gaaatttttg atgagttaac ttggaggtca ttctttcaag atgtcaagcg aatccctcga 1440
agagagtggt ggggggagct ccgtcctaga acaatatgca agatacatga tcttatgcat 1500
gacattgcct tgtctgttat gggaaaagat tgtcttacta tagttgatag gcctaatgaa 1560
aaggagttac tgtccactgg tcctacacgt tacttgttct catcatatga gtatatcgga 1620
actctgttgg atgattatct gaagaaacac tctcccgctc tccagacact gctgtatccg 1680
tatccctaca ccagtgactc agcaccacac ttgtcgaagt gcaattatct gcgagcactg 1740
caactcttca gtttgagaaa acttccactc tggccaaggc acttacagca cctgaggtat 1800
cttgatctct caaataacat gttgatcgaa gagcttccca aggaaataag catactgtat 1860
aatctacaga ctcttaacct ttgtaactgc agaaggcttg atcaacttcc agaggatatg 1920
aagtatatgg aaaatctccg tcacctctat acaaatggat gctcatcatt gaagtgcatg 1980
cctccaggcc tcgggcaact cacttctcta cagactctaa catattttgt ggtgagctct 2040
agtcctggtt gcagtactat tagagaacta caggacttga atcttggtgg tgagttagag 2100
ctatctcgtc ttcagtttgc aactgaagta gatgctaaag catgcagcct tggaaataaa 2160
gagaaactta cacatttatc tcttaaatgg ggtgatgaca gcagtgatga attgggccac 2220
cacaggaatg tgctcgatgc tcttaaacct catgctgtcc tggagtttct aagaatacgc 2280
tcctatagag gtactggctt ccccgcatgg gtggtaagta ttaattttct gcagcatttg 2340
accgagctcc agctagatgg ttgtacaatg tgtgaggaat ttccccaatt cggtcaattt 2400
aagtcccttg aagttcttgt tttgaaaagg ttgaacaaac ttcaaagcct atgcaatcac 2460
agttcatctg caatatttcc agcattaaaa gttctcagat taaaaaaatt ggagattttt 2520
gagagatggg tggcaacaga aggagaagag ttagcatttc ctcaacttga gaatgttaaa 2580
attaaggact gcccaaagct agcaattctg cctgaagcac caaaactcaa gtttatagct 2640
ctaaaagaag aaaaggcaca gctatcctta tcaatattta agtccaggta tatggcttgc 2700
ttgtctgggg ttggcctgtc cgtccgtgat acagaagcag caccaagaac agagcttgat 2760
caggattgtg aagtatctct ttcgaacctg ctgttggatg gttgcaactt tttgttctgc 2820
tcaactccat tgcagccaac tgttggggtc tggaaatggt ttggtcagct tgtgcatttg 2880
gaaataaaat cttgtgatat gctcatctac tggcctgaag aagagttccg ttgcttggta 2940
tcattgaata gtttatccat taattcctgc agcaagctag taggacacac ccaggggaaa 3000
ggatgccgta cccgtactca agtaagggat taactcctgc caaatcttaa aaatttaaga 3060
atacatcatt gtggaagttt gacagagctt tttgttctgc cgccatctct cacaagtatt 3120
gatatgctgg attgcaatag tattgagtcc atactggggc aggatgatac agagttggag 3180
agtatactac actttgatac agcatcatca tcggaacact tcaatgacct cacgtccaca 3240
tgtttgctag agcagtcact ttcacccaga attaatcctt tgccatgtct tgattatttg 3300
cgtatagtta gctgtaagaa gcttcgtttc gtgccagtgc agttagatgc actccaattt 3360
ttgtgtattt ctgattgcaa tgggctggag tcgctggatt gtttgggaga cctaccgtca 3420
ctggaatatc tataccttat gggctgcaaa catctggcat ctgtaccagg aagtcttggg 3480
agttactcgg cccttcagaa gctccgcatt gaatactgcc cagctttaaa tatgaagcca 3540
cttcatggac atctccaaca gcggcttgat agccttgaat tgaaggatct atctaaggct 3600
ggttcaagcc atcctaatga aggtaccttt caatttcatt taccccattt tgtatctctt 3660
tttccatctg tatagtttac agatgtacat attgcagcct gtagtttcaa gtttatccaa 3720
ctccttgtat tttcacattt ggtatgtagt gaccgcgtac acagtttctc tgatatggct 3780
attccaatat catttatttg tataccgtaa gtttgacttg gattgacgtc gtcatgtgtg 3840
gccgatctgt attttctatc attttggtaa aagttaatac accatggaag tatgctctct 3900
tgtgtagtga ccgtacacag atcattcttt tatgtctatc ccactatcat ttatttgtaa 3960
agtttgactt gctattaatg ttcacagaga tattctgaca ttgttttcca tgtacagggc 4020
ctaaactatg ggaaccaaaa tcctggaaat atatgattcc ttcactgcgc aagcgtgagt 4080
ctgaatag 4088
<210> 58
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 58
aatgatacgg cgaccaccga 20
<210> 59
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 59
caagcagaag acggcatacg a 21
<210> 60
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 60
cccaaatcaa cacacaccag 20
<210> 61
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 61
tcgcgacgaa tcgtatatgt 20
<210> 62
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 62
caaggctgct attgctgtgt ttggccctga 30
<210> 63
<211> 28
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 63
gagcgtggtc ttgcccaacc ctccatat 28
<210> 64
<211> 26
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 64
gttcttgagc agcttgtatt catccg 26
<210> 65
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 65
caacatagct cccaacttcc g 21
<210> 66
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 66
acttccgcca ttgttgctct c 21
<210> 67
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 67
ctccttctaa ttagatgcat acattagagg 30
<210> 68
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 68
gtagctcaaa ccgtcggtcc 20
<210> 69
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 69
gattcgtcgc gatgagatc 19
<210> 70
<211> 26
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 70
ggcctaaact atgggaacca aaatcc 26
<210> 71
<211> 25
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 71
ggaaatatat ggttccttca ctgcg 25
<210> 72
<211> 23
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 72
gcaagcgtga gtctgaatag agg 23
<210> 73
<211> 22
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 73
cagtggcctg actacaaact cc 22
<210> 74
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 74
cctgctttgg gttgtatcc 19
<210> 75
<211> 25
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 75
gctggattgc aatagtattg agtcc 25
<210> 76
<211> 22
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 76
gagagagaga gagcaacaat gg 22
<210> 77
<211> 28
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 77
atagaatata gatgcacgct agatcttt 28
<210> 78
<211> 28
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 78
ctgaattcgt gatacttgtc ttacttat 28
<210> 79
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 79
gcctaactcc agaccatttc 20
<210> 80
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 80
aacttgactc ccttgaagag g 21
<210> 81
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 81
cgcagatgga tttctgaagg c 21
<210> 82
<211> 22
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 82
atgtgccaaa tatcatagtc cg 22
<210> 83
<211> 18
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 83
caaataacgg cttctggg 18
<210> 84
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 84
atggactcgg caatactcc 19
<210> 85
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 85
aagctccgtg attcctgtaa g 21
<210> 86
<211> 25
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 86
agtactgtaa taattgattc cgtcg 25
<210> 87
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 87
aacaatggcg gaagttctg 19
<210> 88
<211> 24
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 88
atgttctctt cagaggattt gtca 24
<210> 89
<211> 29
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 89
cgtgatactt gtcttactta ttcctagtc 29
<210> 90
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 90
ccgtgctgta taccatgtga 20
<210> 91
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 91
gcgaacttta ctgtcaacgg 20
<210> 92
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 92
caaagatctc atcgcgacg 19
<210> 93
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 93
caatttcatc aagacatgcg 20
<210> 94
<211> 25
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 94
gatgttattt aattatggtt tggcc 25
<210> 95
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 95
aatgccagcc tgttaaacgc 20
<210> 96
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 96
tgtgttagat ccgtgctgct 20
<210> 97
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 97
atgatgtggt gtggttgggc 20
<210> 98
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 98
cttgagtgct gttggtcttc c 21
<210> 99
<211> 23
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 99
ggcaactctg ttctatagct cgt 23
<210> 100
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 100
cttccacatc cccaccttcc 20
<210> 101
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 101
tgccttgaac agatccatcc a 21
<210> 102
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 102
ctgagcatgg agtagcagca 20
<210> 103
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 103
gaatgggtcg ttacgttgcg 20
<210> 104
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 104
atggcggaag ttctggtgag 20
<210> 105
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 105
ttgacgactc ctcctcctct 20
<210> 106
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 106
ttgacgagtc ctcctcctct 20
<210> 107
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 107
ctcatgcata atcggttgcc t 21
<210> 108
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 108
ctcatgcata atccgttgcc t 21
<210> 109
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 109
agatcggacc agcctgacta 20
<210> 110
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 110
aagcccgtca aagtccacat 20
<210> 111
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 111
tccatcacgc acagccaaat 20
<210> 112
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 112
cgggatgtac tggtggtcc 19
<210> 113
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 113
cgttggcgtg ttagagttgc 20
<210> 114
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 114
ggctgtgaag aaagctgtcc 20
<210> 115
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 115
atagggtatg acggcaaggc 20
<210> 116
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 116
aggtgggttg ggaaacttga 20
<210> 117
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 117
agatgggttg ggaaacttga 20
<210> 118
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 118
acagttgcag aagtttgagg c 21
<210> 119
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 119
acagttgcag aggtttgagc t 21
<210> 120
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 120
tgcttcaggt gtgctctcaa 20
<210> 121
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 121
gctagtggcg tctgagatgt 20
<210> 122
<211> 23
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 122
agtttggtgt cattgggttt tcg 23
<210> 123
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 123
cgagatgcgc ctttggttat g 21
<210> 124
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 124
cgtcgcgatg agatctttga 20
<210> 125
<211> 22
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 125
acaacttcat ccacccgaat ca 22
<210> 126
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 126
cgagatgcgc ctttggttat g 21
<210> 127
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 127
tggcggtcaa agatgcgtat 20
<210> 128
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 128
tggatggcat gaaggagcag 20
<210> 129
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 129
tgatcaagct ggcgataggg 20
<210> 130
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 130
atggacagat gtggaggctc 20
<210> 131
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 131
ctgtccactg gtcctacacg 20
<210> 132
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 132
gctctagtcc tggttgcagt 20
<210> 133
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 133
tttgagagat gggtggcaac 20
<210> 134
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 134
aaaggatgcc gtacccgtac 20
<210> 135
<211> 20
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 135
gacatctcca acagcggctt 20
<210> 136
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 136
tgcgctcacc agaacttccg ccattgttgc 30
<210> 137
<211> 19
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 137
gaaattcctg ctgcattgc 19
<210> 138
<211> 2026
<212> DNA
<213> 人工序列
<220>
<223> 衍生自玉米泛素启动子的启动子构建体
<400> 138
atgaggtctc aggaggcggc cgcgtgcagc gtgacccggt cgtgcccctc tctagagata 60
atgagcattg catgtctaag ttataaaaaa ttaccacata ttttttttgt cacacttgtt 120
tgaagtgcag tttatctatc tttatacata tatttaaact ttactctacg aataatataa 180
tctatagtac tacaataata tcagtgtttt agagaatcat ataaatgaac agttagacat 240
ggtctaaagg acaattgagt attttgacaa caggactcta cagttttatc tttttagtgt 300
gcatgtgttc tccttttttt ttgcaaatag cttcacctat ataatacttc atccatttta 360
ttagtacatc catttagggt ttagggttaa tggtttttat agactaattt ttttagtaca 420
tctattttat tctattttag cctctaaatt aagaaaacta aaactctatt ttagtttttt 480
tatttaataa tttagatata aaatagaata aaataaagtg actaaaaatt aaacaaatac 540
cctttaagaa attaaaaaaa ctaaggaaac atttttcttg tttcgagtag ataatgccag 600
cctgttaaac gccgtcgacg agtctaacgg acaccaacca gcgaaccagc agcgtcgcgt 660
cgggccaagc gaagcagacg gcacggcatc tctgtcgctg cctctggacc cctctcgaga 720
gttccgctcc accgttggac ttgctccgct gtcggcatcc agaaattgcg tggcggagcg 780
gcagacgtga gccggcacgg caggcggcct cctcctcctc tcacggcacg gcagctacgg 840
gggattcctt tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa taaatagaca 900
ccccctccac accctctttc cccaacctcg tgttgttcgg agcgcacaca cacacaacca 960
gatctccccc aaatccaccc gtcggcacct ccgcttcaag gtacgccgct cgtcctcccc 1020
ccccccccct ctctaccttc tctagatcgg cgttccggtc catggttagg gcccggtagt 1080
tctacttctg ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg ctgctagcgt 1140
tcgtacacgg atgcgacctg tacgtcagac acgttctgat tgctaacttg ccagtgtttc 1200
tctttgggga atcctgggat ggctctagcc gttccgcaga cgggatcgat ttcatgattt 1260
tttttgtttc gttgcatagg gtttggtttg cccttttcct ttatttcaat atatgccgtg 1320
cacttgtttg tcgggtcatc ttttcatgct tttttttgtc ttggttgtga tgatgtggtc 1380
tggttgggcg gtcgttctag atcggagtag aattctgttt caaactacct ggtggattta 1440
ttaattttgg atctgtatgt gtgtgccata catattcata gttacgaatt gaagatgatg 1500
gatggaaata tcgatctagg ataggtatac atgttgatgc gggttttact gatgcatata 1560
cagagatgct ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg gtcgttcatt 1620
cgttctagat cggagtagaa tactgtttca aactacctgg tgtatttatt aattttggaa 1680
ctgtatgtgt gtgtcataca tcttcatagt tacgagttta agatggatgg aaatatcgat 1740
ctaggatagg tatacatgtt gatgtgggtt ttactgatgc atatacatga tggcatatgc 1800
agcatctatt catatgctct aaccttgagt acctatctat tataataaac aagtatgttt 1860
tataattatt ttgatcttga tatacttgga tgatggcata tgcagcagct atatgtggat 1920
ttttttagcc ctgccttcat acgctattta tttgcttggt actgtttctt ttgtcgatgc 1980
tcaccctgtt gtttggtgtt acttctgcag gaatgagaga ccatga 2026
<210> 139
<211> 2419
<212> DNA
<213> 人工序列
<220>
<223> 衍生自节节麦Sr33启动子的启动子构建体
<400> 139
atgaggtctc aggaggcggc cgctttaaga gtgttgagga tgtgcttact taaatttcaa 60
aagggctatt tagggataat atttggtgaa gcatggctgg tggactgacc ataggtaccc 120
actgaataaa catatcagcc caaaggcaga gggtatccct ccctgtaata cgtactagta 180
ttcgaggagc gtgctaaagt agtatcctct cttgatgctc ctcgtctccc tccactgagt 240
actcccttcc tctcctcatt ctcatctcag tcctctcctc ctccctcgaa taagatagat 300
ccgcccttgt tgctgatctc atctcgcctc ctattggcct cgcccctgcc aactgactgc 360
tctattggtg accgtggact ttttgggtat atatatttcc tgtcgatttg tttccactta 420
aagtagcaat gcttggatct aaaacttatt acttctcctg cttcagattc tgatccattt 480
gtgttgattg aactcagggt gggatcgtgg ggctctgtat ctatctagga tggatattcc 540
aagcaattta ctctcttgag tgctgttggt cttcctctgc tcctcgtctc cgcttcctct 600
cagtctcctc cattgcgctg cctggaacag atcgatccgc cttgttgctg atctcatcca 660
tcctccgtgg agatttcggg tggcagtttc cagtgactat tcagagcacc atactagtgg 720
cctggtaaaa atttccctcc catctttctt gctattctct tcttattaat ctgctgattt 780
gcttgatggt aagttcttac ttattcttcc agatcgagtg aagcgacatc cgccgccgtc 840
ttgtccccta cgacgccgct gtgttctgct ctgtcctcct ctgccggtga agcatcaacg 900
gtgacggcca tccggtgttg acgccgcggc tgtgttctcc tctgccgtga agtatcaaag 960
gtgggtttgg atggatatca cagtttggat tggcaactct gttctatagc tcgttgaaat 1020
taaagcttca cacagttttg attggattac agctccccat attcatctat tttgcaccat 1080
gcatcgaatc cactgacctc cacacgctca cggatctggc ctctggtgcc cacacgctca 1140
cggatccgac agagtgctcc cttcctatca tctcagtcct catctcctgg tcgatccggc 1200
ttcttgtttg gtgagctcgt ccaacctccg agctcgcccc tgacacctgt ggagtggagg 1260
tatatttctt tccagctgaa gtacgcttgg atctaaaaac ttacgtctcc tgcttcactg 1320
cttgcttggg agtctgatcg atttgtgttg gttgaatcat ttggggtggc agtggagcag 1380
ctagcaagaa tcatggagtt tccaatcatt attcagagca ccacgcatgg aatccactga 1440
cctggtaact ttgtctcatc tttcttgcta ctgaaatctt ccacatcccc accttccttg 1500
ttcatctgct gatttcccag attatacttc cagattgagt gaagcgccgt cttgtcccct 1560
cacggatctg gcctctggtg cccagccgcc gctttcgctt cgttgtccga cgaggccacc 1620
gccgctgtgt tctgctctgc cgtgaagcac aaaaaggtgg ggtcttcctc tgctctttct 1680
ccactgacgg cgatccgacc ttgacacccg tggccgtggc agtggggtct tcctctgctc 1740
tgttttttta ccagttattg gatctaaaaa gggatctaaa agttattact tctcctgctt 1800
gagattctga ccgatttgtg ttgattgaat ccagggtggg gtcgtggggc tctgtatcta 1860
tctagtacgg agtaccactg tattccaagc aatttactct cttgactgct gttggacttc 1920
ctctgtcccc gcttccattg tgctgccttg aacagatcca tccaccttgt tgctaatctc 1980
atccaacctc cgggtatgtt tcttgtcgat ttgtttccag taaagtagca atgcttgggt 2040
ctaaaaagtc attgcttctc ctgcttgacg agtctcattg atttgcccag tcattattca 2100
gagcaccatg catccaaggc gctgacccgg ctataagttt ttattacagg gccgccgtgt 2160
tgtccccatc gagcaaagcg tttaatccac tgacccgctt cgttgtccgc acagcgtgac 2220
catccgacga agacgccgcc gttgtgttct gctctcctct gaagcatcaa aaggtgggct 2280
tggtccagat ccccacattc atcgatttgg cttgccaact cagttccacc agagcttgga 2340
ttgtgttaca gatccctaaa ttcatcgatt tgcaggtttc agctgattga tcaagagagc 2400
tctcaatgag agaccatga 2419
<210> 140
<211> 1277
<212> DNA
<213> 人工序列
<220>
<223> 衍生自栽培一粒小麦编号PI190945的Sr22基因的启动子的启动子构建体
<400> 140
atgaggtctc aggaggcggc cgcaactacc tgggtgactg tatttatcct cagtggacca 60
ctattgtcaa gacagtaccc aaccatgtcg gagagaagag gaaaagattt gcccaaaagc 120
aagagagtgc taggaaggat gtcgagcgtg cctttggtgt tttgcaatct cgatggggca 180
tcgttcggta tcctgctaat acctggagca cgcagaaact gtgggaggtg atgactgctt 240
gtgtgatcat gcacaatatg atcgtagaag acaagcgccc ggaacgtctg tacgatcaag 300
ggtttcagtt tcaggatgag aatgttgtgc ctgagcatgg agtagcagca acgtttgaat 360
agttcaccca atttcatcaa gacatgcgtg attgggaaac tcacgtgcag ctgcaaaata 420
atttgattga gtatatgtgg gtcatgttgg caaccaataa atgtatcttc ttttattcgt 480
ttgcaaaact atgaaacatt tatttttatt tgtatccggt ttgtaaaact atactatttt 540
attctgtaaa ctatgttatc tgacaatatg tttgaatgca aactaatata atattgggcg 600
gccaaccggc cgcgttggca cgttgggtgc gctgccgact caaatctaaa aaaagacgga 660
cgccgggcgg gcggccgacc caaacgaaca aaaagcggac gaaatcgccg tccatttggg 720
tcgctccatc ggagttgctc tgtctggccg ccaaatgggg acgtgacgga caccgagtga 780
actcgtgcgg tgtgtccgct tcacatctaa atttaggtaa aaaaatgaat ccgtatggac 840
atgttttgag aatgggtcgt tacgttgcga caatattttt atccacaacg acctaaacaa 900
atgcgggcag atgaaatgta tggctgcgtt ggagatgctg ttaacggagc aaagtcgttg 960
gtagtcggac cgagcaaagt aattgctgag gcaggtagtg gacgagactg tagaaaggtt 1020
ccacgcttaa tatcttgagc agtacttgta gcacatgtgt ccctgcaaag ctgtctccgt 1080
gctgtatacc atgtgaattg cttttctcaa aagtagtgga gtaccctacc gtgtttcatt 1140
agtcttgccg gcttcataga caaaggattc ttttttcccg tgccatcgcc atctgcaagc 1200
ccaaatcaac acacaccaga gagagagaga gagagagaga gagagagaga gagagagcaa 1260
caaatgagag accatga 1277
<210> 141
<211> 2981
<212> DNA
<213> 人工序列
<220>
<223> 编码区-1_Sr22_基因座_构建体
<400> 141
atgaggtctc aaatgatggc ggaagttctg gtgagcgcat ccacaggggc aatgggttcc 60
ctcctgcgga agttgggagc tatgttgacg gatgaataca agctgctcaa gaacgtccgt 120
ggggacatca agttcctcaa ggacgagctg gaggtcatgt gcgcgttcct cctcaagatg 180
tcagatgtgg aggagcctga cgaaccaaca aagctgcgtg tgacggcggt gcgggagatg 240
tcctataaga tagaggacaa catcgacaag ttcatggtcc tcgtggaaca ggaacacggg 300
tcctcctgtt ccgaggcggc tcatggcgtc gccaagctca tggacaagtg caagaacttg 360
ctgccggaca tcaaggcccg ccgcaggatc gccaaggagg tcaaagacat caagaaagaa 420
attaaggatg tcagcgacag attttcaagg taccagatct tgttctcctt acagtactat 480
tagtacctac catcagattt gtgaccgatt tgttgccact caaggtacaa gattgacgac 540
tcctcctcct ctatgccggc aaaagacaag gtcgaccctc gacttcgtgc agtctacaaa 600
gacgccgcag agctcgtcgg aattgatgga ccaaaggatg aacttgtcaa gtggctgaat 660
gagaaagaag gccaatcact gaaatctgtc tctattgttg gatatggagg gttgggcaag 720
accacgctcg ccaaccagat ccgggtcaac cttggagcga ccttcgactg cggggctttt 780
gtctcgattt cacgcaagcc tgacatgaag gcgatattaa gatctatatt atcacaaatc 840
accaagaaag acgatgcttg ctctagatta gatgatatac aactcatcat cgacaagatt 900
cgagaattcc tccaagacac aaggtatgta tgtgagatct gctacgaagt aacattgctt 960
aattagctca tgcataatcg gttgcctaga attttgctaa tgaagattgc tttgtgtgat 1020
ttactttcgt cctcaacttg tatgactgcg cttaaacttt caagtcattg tttcttgcaa 1080
cttatgtcga tctgttcact aattaagata tagctaaaat gtgtttgccg atttctgtcg 1140
ccacacattt tctaatttga ttgccacaag tttgttaaac ttgcggctat gttctgttta 1200
ttaaataatt gtgacatggc gtcagtcaaa acaataaagt acatgcaatt ccctctcttt 1260
tgtacaagat aaatatccgc acgcactaca tgactgcaaa tatatctatc ttctaaggac 1320
ccaaagcact tgcatgtgca gaccattgtt accggatatg cagtacatta ggtttgtgat 1380
tagccacttt aagaacatag ggtttctaga ggctataaac ctacggttat cagtattata 1440
atcttgaatg gatatcaaac atccgttgaa gaatctatgc ccataacaca aaaagagaaa 1500
agggaagaat taatcatagt cagatcggac cagcctgact agcgtaacat aatgacaaca 1560
gtaggttgtt gcgtctttcc tggtttcgta gcaatttgca tgacgaattg aggcgcagag 1620
ccaccactca atatgtggtc ctaccaatca tgaggaaata acttgttacc aaaatgacaa 1680
aagaaaatag gtagtcccca tcattgtttc aatatctatc ctcttgcgaa catgctattg 1740
gcagtggcaa aacattgtac cttgccactg gcaacagctg cctttactcg gcctgagcat 1800
tcatattttt ttcctcttgg ttgttaaatc ttgctatttt tcgcttgcct tctatggcaa 1860
tctttttttt tcttatattg ccaccaaatt gtatctaaga gcaactccaa cacgccgacg 1920
caatttttgt ccatttagat cggccaagcg gacggggtgt ctggttttgc ctttgggtgg 1980
tgcgcctacg cattagtccg cgtgggcatt taaaaaactt aaaaagctta aaataattaa 2040
atataaataa aaatgcatat taaagcccgt caaagtccac ataattaaac ataatacatt 2100
aatatattaa taaaaaacag ccgccccagc ccgcagccct aggcgtcttc gtcggaggag 2160
tgggtgaggt cgaagaatgc cggcagctca gcccacggga aggcctgctg gtgggcagcc 2220
agcaccgcct gctccgctgt ctgcggcggc ggcggcccta cctggcaccg cgcacgcctc 2280
ctcctcgcgc tcctgccgct cctcctcctc atcgcacgcc caacgaagtt ttggcgctcc 2340
tccccctcgg gttcccgctg catgcggacc tggccgtcgt accgctcctc ctccagcgtg 2400
cggtccttcc acgccacgag gtaggccgcc tcctcctcca tcacgcacag ccaaatgggc 2460
ggcttgctca tccgcttgca gacgatgccc tgccattggt aaacgccggt gggcgcgggt 2520
ggtgctagcg ctggtgcagg cggtgacgat gtggctggtg gtcccggcag gtagacacta 2580
tagctggcgg ccgatagctg cagcgtgtca tcgaggccag accactagga gtcctcgcac 2640
taagactcct cggtgacgtg atgggccccc tcctccaatg ccttgaggaa cgccgcctcc 2700
tcctcaacct actatttgac caccggtggc ggcggtggag ggcaggcttc gagctggacg 2760
gcgtcgcgga ggcgcacatg gtgctcgatg gtgaaccaga cttctcagtt aggggagtct 2820
ggcctcgatg ggtggagtcg cgacgctgct ctggcgtcag gagagcacgc cgggcccgaa 2880
tctcgttggc gcgcgcacgc tcggactgtg gcaccgccgg cactggaatg tggtgggggt 2940
cgaggtgcca gccgtggggg aagttcacgt agagaccatg a 2981
<210> 142
<211> 2997
<212> DNA
<213> 人工序列
<220>
<223> 编码区-2_Sr22_基因座_驯化的_BsaI_构建体
<400> 142
atgaggtctc aacgtccggc cacggcagtg agacgcggta ctcccggtgg tactaactac 60
gcgtggtgca ccgggatgta ctggtggtcc cgtggcgctg ccgccctccc atcccgcagc 120
gccgacacca atgcgaaccc cttcgctgcc ggcctcaccg gcccttgcaa cgagccggcc 180
tcgtggtcgt gcttgccttg cccttacctt tgccaaagaa acccatgacg gctagggttt 240
tctgtcaccg gcgagggagc agatgtggac tagagtggga ggagaagtgg atgaggcccg 300
ccccgccgca cccttgcaat taaaaagggc gtgatccgga cgtttctagt cactgccatg 360
cgggcccgag gtaggtgggc gccattgact acgtctgtgg tggtagttgg ctggcagaca 420
cgccagcccg aggcgcacac cgagcggacg cgcaacacgt ccgtctcgtg ttcgtgtgaa 480
cgcaaatcag gcacaaattt gggctggaaa tgcgtctaca tggatgccga acggacgtga 540
tttgggtttg tgtccgcacg ttgggccggc gtttttgtgc ctttcgaccc taatggacac 600
acacggacca aatccgttgg cgtgttagag ttgcttgtac ctagtactac tatgtgtgga 660
gttgttatct ttgagtttct cccccatctg tagctgtgta ttattgtgag atgctctcag 720
gatttcactt attttagata atgacaactt tcttgatgct agacaattac atcaaggtgt 780
attatatcta ggatgcacac agatgtgaga ttgtttcagg attttaggtt acttggtagg 840
tgttactata aatatctaat gagctctaat gtgtatgttc atgaaactgt gttgcaggta 900
ctttatcata attgatgata tatgggagtt aggaacatgg gaaactttga aatgtgcatt 960
tgtcaagaat acattgggca gcagaataat tatcacaaca cgtatagttg atgttgccaa 1020
atcttgttct ccttctagtg aagatcttgt ctatgagatg aaaccactca gtgaggctga 1080
ctcgaagaaa ttgtttttca agaggatatt tggctgtgaa gaaagctgtc ctgatagctt 1140
gaaagaagct gccaatgata ttttgaaaaa atgccgtggc ctacctctgg ccatcaatgc 1200
catttctagc gtgttggtca caacaaggga aacaaaagaa gagtgggatc gggtgcgaca 1260
ttcaatacgc tcttcaaagg tcaaaagtga tataatagaa accatgaatt acatattgtc 1320
tcttagttat tttgatcttc cccaccacct aagaagctgc ttattgtacc tggctctgtt 1380
tcctgaagac caattgattg gaaggaagcg gttagtacgc agatggattt ctgaaggctt 1440
tatccatgga gaaagtggac aagatctcat ggaattagga gaggaatatt ttcaccagct 1500
tgtgaacaga agtctaatac agcccggcaa catagggtat gacggcaagg caatgtattg 1560
ccgagtccat gacaccatcc ttgatttcct aattgacaaa tcctctgaag agaacatgtg 1620
tactgtccta aagaaacaat gcaagcctaa tggcatagtt cgtcggctct ctctaatggg 1680
gaatgaggat gaagaaatcg tggagcaatt ggatctgtca cacgctcggt caatcactgc 1740
ttttggggat atcaagctat tgccttcgct tgggaggtca aaatgcttgc gcgtgctaga 1800
cttgcaagac tgcgatcagt tggaaaatca tcatatcaag gatattgaaa gactctatca 1860
gctaagatac ttggatatct cttctacagg aatcacggag cttcccaggc aaattgggga 1920
gttgctgtat ctagagacgc tagttgccta tggattacgt gagcttcctg aaagcacaag 1980
tcggctacaa cgactggcac gtttgtttgt ttactctggt tgtaagcttc caggtgggtt 2040
gggaaacttg ataaacttgc aagagcttga ctgcgttgat gccttgcatt tgaagcatgt 2100
ggaagagctc ggcaaactaa caaatctgag aaagctgagt attaaattgg acactggtgg 2160
gattgaaggc aacaaattag aagagtccaa ggaaaagctg gtgtcctctc tctgtaaact 2220
ggacgaatgc ggcctccttt ccctgagtat tgattattat ctgagagaaa aggatggaga 2280
agagccgttc ctccctgctt tgggttgtat ccaggaggtt tttgtatatg gacaagatat 2340
cagccggatt agcagatggc tcgcgtcact tcccaaccta cacaggttgc ttttggacga 2400
tccgaagata gagcagcagg atattgagat gattggattg atacccaatc tgatcgattt 2460
gacactgccg cctctataca aaacagacga cgctgggcgg ctcatcatca ggcgtgaagg 2520
atttcaacag ttgcagaagt ttgaggctta caatactcgt atgggggttt taatgtttga 2580
accgggggct atgccgaggc tcaaggagct taaactccat aacttcatcg aaaagcccaa 2640
atctgctgcg gtcgatttcg actttggcat ccaacgcctc tccagccttg ctcgcctcac 2700
tgtcagctta tcctgcggtg gctggacggt ggcagaagta gaggctgcgg aggatgcttt 2760
caagagcatg gccgaggcga accccaaccg tccaatactg gagatgacaa gatataatac 2820
gcagcatatg ttacaagacg agcaaatagg tatgacaggc tcaggtacta ttaactagtt 2880
tgtacttttg tcttggtgag cagtagatca aaagatgtgc atatgcttca atcttttttc 2940
cggtaatcta tttcagctac aacgccggcc gtcaagtcct aggcttagag accatga 2997
<210> 143
<211> 2974
<212> DNA
<213> 人工序列
<220>
<223> 编码区-1_ PI190945_构建体
<400> 143
atgaggtctc aaatgatggc ggaagttctg gtgagcgcat ccacaggggc aatgggttcc 60
ctcctgcgga agttgggagc tatgttgacg gatgaataca agctgctcaa gaacgtccgt 120
ggggacatca agttcctcaa ggacgagctg gaggtcatgt gcgcgttcct cctcaagatg 180
tcagatgtgg aggagcctga cgaaccaaca aagctgcgtg tgacggcggt gcgggagatg 240
tcctataaga tagaggacaa catcgacaag ttcatggtcc tcgtggaaca cgagtcctcc 300
tgctccgagg cggctcatgg cgtcgccaag ctcatggaca agtgcaagaa cttgctgccg 360
gacatcaaga cccgccgcag gatcgccaag gaggtcaaag acatcaagaa agaaattaag 420
gatgtcagcg acagattttc aaggtaccag atcttgttct ctttacagta ctattagtac 480
ctaccatcag atttgtgacc gatttgttgc cactcaaggt acaagattga cgagtcctcc 540
tcctctatgc cggcaaaagg caaggtcgac cctcgacttc gtgcagtcta caaagacgcc 600
gcagagctcg tcggaattga tggaccaaag gatgaacttg tcaagtggct gaatgagaaa 660
gagggccaat cactgaaatc tgtctctatt gttggatatg gagggttggg caagaccacg 720
ctcgccaacc agatccgggt caaccttgga gcgaccttcg actgcggggc ttttgtctcg 780
atttcacgca agcctgacat gaaggcgata ttaagatcta tattatcgca aatcaccaag 840
aaagacgagg cttgctctag attagatgat atacaactca tcatcgacaa gattcgagaa 900
ttcctccaag acacaaggta tgtatgtgag atctactacg aagtaacatt gcttaattag 960
ctcatgcata atccgttgcc tagaattttg ctaatgagat tgctttgtgt gatttacttt 1020
cgtcctcaac ttgtatgact gcgcttaaac tttcaagtca ttgtttcttg caacttatgt 1080
cgatctgttc actaattaag atatagctaa aatgtgtttg ccaatttctg tcgccacaca 1140
ttttctaatt tgattgccac aggtttgtta aacttgcggc tatgttctgt ttattaaata 1200
attgtgacat ggcgtcagtc aaaacaataa agtacatgca attccctctc ttttgtacaa 1260
gataaatatc cgcacgcact acatgactgc aaatatatct atcttctaag gacccaaagc 1320
acttgcatgt gcagaccatt gttaccggat atgcagtaca ttaggtttgt gattagccac 1380
tttaagaaca tagggtttct agaggctata aacctacggt tatcagtatt ataatcttga 1440
atggatatca aacatccgtt gaagaatcta tgcccataac acaaaaagag aaaagggaag 1500
aattaatcat agtcagatcg gaccagcctg actagcgtaa cataatgaca acagtaggtt 1560
gttgcgtctt tcctggtttc gtagcaattt gcatgacgaa ttgaggcgca gagccaccac 1620
tcaatatgtg gtcctaccaa tcatgaggaa ataacttgtt accaaaatga caaaagaaaa 1680
taggtagtcc ccatcattgt ttcaatatct atcctcttgc gaacatgcta ttggcagtgg 1740
caaaacattg taccttgcca ctggcaacag ctgcctttac tcggcctgag cattcatatt 1800
tttttcctct tggttgttaa atcttgctat ttttcgcttg ccttctatgg caatcttttt 1860
ttttcttata ttgccaccaa attgtatcta agagcaactc caacacgccg acgcaatttt 1920
tgtccattta gatcggccaa gcggacgggg tgtctggttt tgcctttggg tggtgcgcct 1980
acgcattagt ccgcgtgggc atttaaaaaa cttaaaaagc ttaaaataat taaatataaa 2040
taaaaatgca tattaaagcc cgtcaaagtc cacataatta aacataatac attaatatat 2100
taataaaaaa cagccgcccc agcccgcagc cctaggcgtc ttcgtcggag gagtgggtga 2160
ggtcgaagaa tgccggcagc tcagcccacg ggaaggcctg ctggtgggca gccagcaccg 2220
cctgctccgc tgtctgcggc ggcggcggcc ctacctggca ccgcgcacgc ctcctcctcg 2280
cgctcctgcc gctcctcctc ctcatcgcac gcccaacgaa gttttggcgc tcctccccct 2340
cgggttcccg ctgcatgcgg acctggccgt cgtaccgctc ctcctccagc gtgcggtcct 2400
tccacgccac gaggtaggcc gcctcctcct ccatcacgca cagccaaatg ggcggcttgc 2460
tcatccgctt gcagacgatg ccctgccatt ggtaaacgcc ggtgggcgcg ggtggtgcta 2520
gcgctggtgc aggcggtgac gatgtggctg gtggtcccgg caggtagaca ctatagctgg 2580
cggccgatag ctgcagcgtg tcatcgaggc cagaccacta ggagtcctcg cactaagact 2640
cctcggtgac gtgatgggcc ccctcctcca atgccttgag gaacgccgcc tcctcctcaa 2700
cctactattt gaccaccggt ggcggcggtg gagggcaggc ttcgagctgg acggcgtcgc 2760
ggaggcgcac atggtgctcg atggtgaacc agacttctca gttaggggag tctggcctcg 2820
atgggtggag tcgcgacgct gctctggcgt caggagagca cgccgggccc gaatctcgtt 2880
ggcgcgcgca cgctcggact gtggcaccgc cggcactgga atgtggtggg ggtcgaggtg 2940
ccagccgtgg gggaagttca cgtagagacc atga 2974
<210> 144
<211> 2994
<212> DNA
<213> 人工序列
<220>
<223> 编码区-2_PI190945_驯化的_BsaI_构建体
<400> 144
atgaggtctc aacgtccggc cacggcagtg agacgcggta ctcccggtgg tactaactac 60
gcgtggtgca ccgggatgta ctggtggtcc cgtggcgctg ccgccctccc atcccgcagc 120
gccgacacca atgcgaaccc cttcgctgcc ggcctcaccg gcccttgcaa cgagccggcc 180
tcgtggtcgt gcttgccttg cccttacctt tgccaaagaa acccatgacg gctagggttt 240
tctgtcaccg gcgagggagc agatgtggac tagagtggga ggagaagtgg atgaggcccg 300
ccccgccgca cccttgcaat taaaaagggc gtgatccgga cgtttctagt cactgccatg 360
cgggcccgag gtaggtgggc gccattgact acgtctgtgg tggtagttgg ctggcagaca 420
cgccagcccg aggcgcacac cgagcggacg cgcaacacgt ccgtctcgtg ttcgtgtgaa 480
cgcaaatcag gcacaaattt gggctggaaa tgcgtctaca tggatgccga acggacgtga 540
tttgggtttg tgtccgcacg ttgggccggc gtttttgtgc ctttcgaccc taatggacac 600
acacggacca aatccgttgg cgtgttagag ttgcttgtac ctagtactac tatgtgtgga 660
gttgttatct ttgagtttct cccccatctg tagctgtgta ttattgtgag atgctctcag 720
gatttcactt attttagata atgacaactt tcttgatgct agacaattac atcaaggtgt 780
attatatcta ggatgcacac agatgtgaga ttgtttcagg attttaggtt acttggtagg 840
tgttactata aatatctaat gagctctaat gtgtatgttc atgaaactgt gttgcaggta 900
ctttatcata attgatgata tatgggagtt aggaacatgg gaaactttga aatgtgcatt 960
tgtcaagaat acattgggca gcagaataat tatcacaaca cgtatagttg atgttgccaa 1020
atcttgttct ccttctagtg aagatcttgt ctatgagatg aaaccactca gtgaggctga 1080
ctcgaagaaa ttgtttttca agaggatatt tggctgtgaa gaaagctgtc ctgatagctt 1140
gaaagaagct gccaatgata ttttgaaaaa atgccgtggc ctacctctgg ccatcaatgc 1200
catttctagc gtgttggtca caacaaggga aacaaaagaa gagtgggatc gggtgcgaca 1260
ttcaatacgc tcttcaaagg tcaaaagtga tataatagaa accatgaatt acatattgtc 1320
tcttagttat tttgatcttc cccaccacct aagaagctgc ttattgtacc tggctctgtt 1380
tcctgaagac caattgattg gaaggaagcg gttagtacgc agatggattt ctgaaggctt 1440
tatccatgga gaaagtggac aagatctcat ggaattagga gaggaatatt ttcaccagct 1500
tgtgaacaga agtctaatac agcccggcaa catagggtat gacggcaagg caatgtattg 1560
ccgagtccat gacaccatcc ttgatttcct aattgacaaa tcctctgaag agaacatgtg 1620
tactgtccta aagaaacaat gcaagcctaa tggcatagtt cgtcggctct ctctaatggg 1680
gaatgaggat gaagaaatcg tggagcaatt ggatctgtca cacgctcggt caatcactgc 1740
ttttggggat atcaagctat tgccttcgct tgggaggtca aaatgcttgc gcgtgctaga 1800
cttgcaagac tgcgatcagt tggaaaatca tcatatcaag gatattgaaa gactctatca 1860
gctaagatac ttggatatct cttctacagg aatcacggag cttcccaggc aaattgggga 1920
gttgctgtat ctagagacgc tagttgccta tggattacgt gagcttcctg aaagcacaag 1980
tcggctacaa cgactggcac gtttgtttgt ttactctggt tgtaagcttc caggtgggtt 2040
gggaaacttg ataaacttgc aagagcttga ctgcgttgat gccttgcatt tgaagcatgt 2100
ggaagagctc ggcaaactaa caaatctgag aaagctgagt attaaattgg acactggtgg 2160
gattgaaggc aacaaattag aagagtccaa ggaaaagctg gtgtcctctc tctgtaaact 2220
ggacgaatgc ggcctccttt ccctgagtat tgattattat ctgagagaaa aggatggaga 2280
agagccgttc ctccctgctt tgggttgtat ccaggaggtt tttgtatatg gacaagatat 2340
cagccggatt agcagatggc tcgcgtcact tcccaaccta cacgggttgc ttttggacaa 2400
tccgaagata gagcagcagg atattgagat gattggattg atacccaatc tgatcgattt 2460
gacactgtat ttacgcataa cagacgacgc tgggcggctc atcatcaagc gtgaaggatt 2520
tcaacagttg cagaggtttg agctttcccg tattcgtatg ggggatttaa tgtttgaacc 2580
gggggctatg ccgaggctca aggagcttat actctaccac ttcatcgaaa agcccaaatc 2640
tggtgcggtc gatttcgact ttggcatcca acacctctcc agccttgctc gcctcactgt 2700
cggcttatta tgcgttggct cgacggcggc ggaagtagag gctgcggagg atgctttcaa 2760
gagcatggcc gaggcgaacc ccaaccgtcc aatactggag atgacaagac tttatccgca 2820
gcatatgtta caagacgagc aaataggtat gacaggctca ggtactatta actagtttgt 2880
acttttgtct tggtgagcag tagatcaaaa gatgtgcata tgcttcaatc ttttttccgg 2940
taatctattt cagctacaac gccggccgtc aagtcctagg cttagagacc atga 2994
<210> 145
<211> 2975
<212> DNA
<213> 人工序列
<220>
<223> 编码区-1_PI573523_构建体
<400> 145
atgaggtctc aaatgatggc ggaagttctg gtgagcgcat ccacaggcgc aatgggttcc 60
ctcctccgga agttgggagc tatgttgacg gatgaataca agctgctcaa gaacgtccgt 120
ggggacatca agttcctcaa ggacgagctg gaggtcatgt gcgcgttcct cctcaagatg 180
tcagatgtgg aggagcctga cgaaccaaca aagctgcgtg tgacggcggt gcgggagatg 240
tcctataaga tagaggacaa catcgacaag ttcatggtcc tcgtggaaca cgagtcctcc 300
tgctccgagg cggctcatgg cgtcgccaag ctcatggaca agtgcaagaa cttgctgccg 360
gacatcaaga cccgccgcag gatcgccaag gaggtcaaag acatcaagaa agaaattaag 420
gatgtcagcg acagattttc aaggtaccag atcttgttct ctttacagta ctattagtac 480
ctaccatcag atttgtgacc gatttgttgc cactcaaggt acaagattga cgagtcctcc 540
tcctctatgc cggcaaaagg caaggtcgac cctcgacttc gtgcagtcta caaagacgcc 600
gcagagctcg tcggaattga tggaccaaag gatgaacttg tcaagtggct gaatgagaaa 660
gagggccaat cactgaaatc tgtctctatt gttggatatg gagggttggg caagaccacg 720
ctcgccaacc agatccgggt caaccttgga gcgaccttcg actgcggggc ttttgtctcg 780
atttcacgca agcctgacat gaaggcgata ttaagatcta tattatcaca aatcaccaag 840
aaagacgatg cttgctctag attagatgat atacaactca tcatcgacaa gattcgagaa 900
ttcctccaag acacaaggta tgtatgtgag atctgctacg aagtaacatt gcttaattag 960
ctcatgcata atcggttgcc tagaattttg ctaatgaaga ttgctttgtg tgatttactt 1020
tcgtcctcaa cttgtatgac tgcgcttaaa ctttcaagtc attgtttctt gcaacttatg 1080
tcgatctgtt cactaattaa gatatagcta aaatgtgttt gccgatttct gtcgccacac 1140
attttctaat ttgattgcca caagtttgtt aaacttgcgg ctatgttctg tttattaaat 1200
aattgtgaca tggcgtcagt caaaacaata aagtacatgc aattccctct cttttgtaca 1260
agataaatat ccgcacgcac tacatgactg caaatatatc tatcttctaa ggacccaaag 1320
cacttgcatg tgcagaccat tgttaccgga tatgcagtac attaggtttg tgattagcca 1380
ctttaagaac atagggtttc tagaggctat aaacctacgg ttatcagtat tataatcttg 1440
aatggatatc aaacatccgt tgaagaatct atgcccataa cacaaaaaga gaaaagggaa 1500
gaattaatca tagtcagatc ggaccagcct gactagcgta acataatgac aacagtaggt 1560
tgttgcgtct ttcctggttt cgtagcaatt tgcatgacga attgaggcgc agagccacca 1620
ctcaatatgt ggtcctacca atcatgagga aataacttgt taccaaaatg acaaaagaaa 1680
ataggtagtc cccatcattg tttcaatatc tatcctcttg cgaacatgct attggcagtg 1740
gcaaaacatt gtaccttgcc actggcaaca gctgccttta ctcggcctga gcattcatat 1800
ttttttcctc ttggttgtta aatcttgcta tttttcgctt gccttctatg gcaatctttt 1860
tttttcttat attgccacca aattgtatct aagagcaact ccaacacgcc gacgcaattt 1920
ttgtccattt agatcggcca agcggacggg gtgtctggtt ttgcctttgg gtggtgcgcc 1980
tacgcattag tccgcgtggg catttaaaaa acttaaaaag cttaaaataa ttaaatataa 2040
ataaaaatgc atattaaagc ccatcaaagt ccacataatt aaacataata cattaatata 2100
ttaataaaaa acagccgccc cagcccgcag ccctaggcgt cttcgtcgga ggagtgggtg 2160
aggtcgaaga atgccggcag ctcagcccac gggaaggcct gctggtgggc agccagcacc 2220
gcctgctccg ctgtctgcgg cggcggcggc cctacctggc accgcgcacg cctcctcctc 2280
gcgctcctgc cgctcctcct cctcatcgca cgcccaacga agttttggcg ctcctccccc 2340
tcgggttccc gctgcatgcg gacctggccg tcgtaccgct cctcctccag cgtgcggtcc 2400
ttccacgcca cgaggtaggc cgcctcctcc tccatcacgc acagccaaat gggcggcttg 2460
ctcatccgct tgcagacgat gccctgccat tggtaaacgc cggtgggcgc gggtggtgct 2520
agcgctggtg caggcggtga cgatgtggct ggtggtcccg gcaggtagac actatagttg 2580
gcggccgata gctgcagcgt gtcatcgagg ccagaccact aggagtcctc gcactaagac 2640
tcctcggtga cgtgatgggc cccctcctcc aatgccttga ggaacgccgc ctcctcctca 2700
acctactatt tgaccaccgg tggcggcggt ggagggcagg cttcgagctg gacggcgtcg 2760
cggaggcgca catggtgctc gatggtgaac cagacttctc agttagggga gtctggcctc 2820
gatgggtgga gtcgcgacgc tgctctggcg tcaggagagc acgccgggcc cgaatctcgt 2880
tggcgcgcgc acgctcggac tgtggcaccg ccggcactgg aatgtggtgg gggtcgaggt 2940
gccagccgtg ggggaagttc acgtagagac catga 2975
<210> 146
<211> 2998
<212> DNA
<213> 人工序列
<220>
<223> 编码区-2_PI573523_驯化的_BsaI_构建体
<400> 146
tgaggtctca acgtccggcc atggcagtga gacgcggtac tcccggtggt actaactacg 60
cgtggtgcac cgggatgtac tggtggtccc gtggcgctgc cgccctccca tcccgcagcg 120
ccgacaccaa tgcgaacccc ttcgctgccg gcctcaccgg cccttgcaac gagccggcct 180
cgtggtcgtg cttgccttgc ccttaccttt gccaaagaaa cccatgacgg ctagggtttt 240
ctgtcaccgg cgagggagca gatgtggact agagtgggag gagaagtgga tgaggcccgc 300
cccgccgcac ccttgcaatt aaaaagggcg tgatccggac gtttctagtc actgccatgc 360
gggcccgagg taggtgggcg gcattgacta cgtctgtggt ggtagttggc tggcagacac 420
gccagcccga ggcgcacacc gagcggacgc gcaacacgtc cgtctcgtgt tcgtgtgaac 480
gcaaatcagg cacaaatttg ggctggaaat gcgtctacat ggatgccgaa cggacgtgat 540
ttgggtttgt gtccgcacgt tgggccggcg tttttgtgcc tttcgaccct aatggacaca 600
cacggaccaa atccgttggc gtgttagagt tgcttgtacc tagtactact atgtgtggag 660
ttgttatctt tgagtttctc ccccatctgt agctgtgtat tattgtgaga tgctctcagg 720
atttcactta ttttagataa tgacaacttt cttgatgcta gacaattaca tcaaggtgta 780
ttatatctag gatgcacaca gatgtgagat tgtttcagga ttttaggtta cttggtaggt 840
gttactataa atatctaatg agctctaatg tgtatgttca tgaaactgtg ttgcaggtac 900
tttatcataa ttgatgatat atgggagtta ggaacatggg aaactttgaa atgtgcattt 960
gtcaagaata cattgggcag cagaataatt atcacaacac gtatagttga tgttgccaaa 1020
tcttgttctc cttctagtga agatcttgtc tatgagatga aaccactcag tgaggctgac 1080
tcgaagaaat tgtttttcaa gaggatattt ggctgtgaag aaagctgtcc tgatagcttg 1140
aaagaagctg ccaatgatat tttgaaaaaa tgccgtggcc tacctctggc catcaatgcc 1200
atttctagct tgttggtcac aacaagggaa acaaaagaag agtgggatcg ggtgcgacat 1260
tcaatacgct cttcaaaggt caaaagtgat ataatagaaa ccatgaatta catattgtct 1320
cttagttatt ttgatcttcc ccaccaccta agaagctgct tattgtacct ggctctgttt 1380
cctgaagacc aattgattgg aaggaagcgg ttagtacgca gatggatttc tgaaggcttt 1440
atccatggag aaagtggaca agatctcatg gaattaggag aggaatattt tcaccagctt 1500
gtgaacagaa gtctaataca gcccggcaac atagggtatg acggcaaggc ggagtattgc 1560
cgagtccatg acaccatcct tgatttccta attgacaaat cctctgaaga gaacatgtgt 1620
actgtcctga agaaacaatg caagcctaat ggcatagttc gtcggctctc tctaatgggg 1680
aatgaggatg aagaaatcat ggagcaattg gatctgtcac acgctcggtc aatcagtgct 1740
tttggggata tcaagctatt gccttcgctt gggaggtcaa aatgcttgcg cgtgctagac 1800
ttgcaaggct gctatcagtt gaaaaatcat catatcaagg atattgaaag actctatcag 1860
ctgagatact tggatatctc ttttacagga atcacggagc ttcccaggca aattggggag 1920
ttgctgtatc tagagacgct agttagtaca tcctatggat tacgtgagct tcctgaaagc 1980
acaagtcggc tacaacgact ggcacgtttg tttgttgaca gcggttgtaa gcttccagat 2040
gggttgggaa acttgataaa cttgcaagag cttgactgcg ttgatgcctt gcagttgaag 2100
catgtggaag agctcggcaa actaacaaat ctgagaaagc tgaggattaa attggacact 2160
gatgggattg aaggcaacaa attagaacag tccaaggaaa agctggtgtc ctctctctgt 2220
aaactggacg aatgcggcct cctttccctg agtatttatt attatctgag agaaaaggat 2280
ggagaagagc cgttcctccc tgctttgggt tgtatccagg aggtttttgt acatggacaa 2340
gatatcagcc ggattagcag atggctcgcg tcacttccca acctacacag gttgtttttg 2400
aaccaaccga agatagagca gcaggatatt gagatgattg gattgatacc caatctgatg 2460
gatttgacac tgtatttatg cataacagac gacgctgggc ggctcatcat caagcgtgaa 2520
ggatttcaac agttgcagag gtttgagctt tcccgtattc gtatggggga tttaatgttt 2580
gaaccggggg ctatgccgag gctcaaggag cttatactct acgacttcat cggaaagccc 2640
aaatctggtg cggtcgattt cgactttggc atccaacacc tctccagcct tgctcgcctc 2700
actgtcggct tatcctgcgt tggctcgacg gtggcggaag tagaggccgc ggaggatgct 2760
ttcaagagca tggccgaggc gaaccccaac cgtccaatac tggagatgac aagatataat 2820
acgcagcata tgttacaaga cgagcaaata ggtatgacag gctcaggtac tattaactag 2880
tttgtacttt tgtcttggtg agcggtagat caaaagatgt gcatatgctt caatcttttt 2940
tccggtaatc tatttcagct acaacgccgg ccgtcaagtc ctaggcttag agacctga 2998
<210> 147
<211> 1459
<212> DNA
<213> 人工序列
<220>
<223> 终止子_Sr33_天然_构建体
<400> 147
atgaggtctc agcttctgac gttgcttcag gtgtgctctc aatctataca tatttactca 60
tcatattatt ctccaccttt ttttccatct ctagagctca gcttgtcatt gcattgttca 120
attgtgcttc tctagcagct acggaaattc tgaacaggca aaggtaattt tacagaagtt 180
tctttggcat aagtatctga gaaaaatgca cttggcacaa tttacctttt cgcagtagaa 240
aagagtcagt ggatcatacc ttcttaattg ttcgattgct cgtaatactt gggggcactc 300
tatgattgct gtgtgctttt agttttgata tagttcctgt tagcatggac agactgtgtg 360
attggttcat gaatttcagg gtggttctaa tgaggaaatg atgcttgctt cagaaatagt 420
ttccatttgt acctaagctg tgtagttggt aaagttgcat tttggatgtt ctgttggtat 480
agcttacaga agaaagactt gcgttgtgca ggtaccaggg gcgcaggtta ctgctagtgg 540
cgtctgagat gtttcataaa cgttgggtgg gcaccggtca tgcaaaaaat tgaaggataa 600
aaaggttccg agaggttgtg tgatgtgctc cttttaaatg gcagtcgctt gcataaagag 660
atgttgcttg gttttttagt aaagcgttgt agtaagttgc tctgtttcgg ctgagagcca 720
tcgttgttgc tgtctgttac atcgaatggc tggctcatgt catgttgatg ttttttttat 780
ctgaaccgga caaacctgag ggtgtagtat catgtttgta atgactgaaa ttctgggctc 840
acccgagctc cttaattcgt aaacaactgc gactcattgt tggattcctt acttcagaaa 900
atatttactt gctctggctc atacagctct gcatgttttg tagtggtttg tcgctgttta 960
ggtgctgctt cagactcctg gtacctttgg ccgctgctgc tactgcatga gctttgtatg 1020
gcattgttag tatagtttgg tgtcattggg ttttcgcccc atggtgagtg tccattgcgc 1080
gcactcgcag cggtttcctg gcgataagtt gaacttgatc tttctctttc ccacctccta 1140
aacgtctatt cagacttgtg tatatttgca ttacggggat tacccgagtt caaaaataaa 1200
tattatttac tagttctgcc aatgtgtttc cgttacatac aaataaaagg gtgcttccag 1260
ctacaagtat atttttgaga agggccattg caaaacgaaa tgaagcccaa attctaccaa 1320
catgtttctg tcacatatac attaaaaaaa cggtgtaaat gtcccgtcaa aaaagggtgt 1380
atgtaggaaa aggagttcga cgccgccagc tccctcctaa cgctcagatt gtcttcgcgg 1440
ccgccgctag agaccatga 1459
<210> 148
<211> 1598
<212> DNA
<213> 人工序列
<220>
<223> 终止子_Schomburgk_驯化的_BsaI_构建体
<400> 148
atgaggtctc agctttgcct aaaccgagat gcgcctttgg ttatggagcc caagtcacgt 60
actcggattg gatgctgcag caaatgatac ttctgtacat gtctgcaagt atcacgaatt 120
agtggaatga ctaggaataa gtaagacaag tatcacgaat tcagagtcgg atgcaatttt 180
tttcttttct tttgggcata agtagattgt catttgagta tttttttcca ttaatatttg 240
tgtattctta gattgagact ttgatatgta acccagattt ctgttgttag gaaacacata 300
ccgtgtagct tatcagaaga aaaagatcta gcgtgcatct atattctatc ggactatgat 360
atttggcaca tgtgtgccat ataaacaaaa ctgtcacacc tctatttcct tcactctaaa 420
tagatgtata cattagtttg attctttctt ctttcacgtg catttctcct tctaattaga 480
tgcatacatt agaggacaaa aaagacgatg tcattctaga ttttctttat taaaatattt 540
aaaatgtttt gtagctcaaa ccgtcggtcc gatgtaaaaa ccgtttttac atatacgatt 600
cgtcgcgatg agatctttga aactagatcc tatgttgaca tgtttcgatg acttttttta 660
accaaaattt accatgcctt ttatatgcaa gttaccgtgc tatttccaca taagtcatcg 720
gaggcataaa aatggttcac cgacactaca gttcatatat taccatgatg tttacatata 780
agttaccggg aggggggggg ggggtgtctg ttttcaataa tttccccacc gagtcaaata 840
ttaccatagt atatgtacgt aagttatcag gtatccgatg ggtatattac catggctatc 900
ttttaaaaaa atctctaatt taccgcggtg tttgtatcta aattatcaag tttgtggtgc 960
ctaatttact atgctattta catacaaaag ttaccagtgt attttttgac aattttttac 1020
tattcttccc caaatcaaga ctattaccgt gctgtttaca cacaagttac cgggtatatt 1080
ttcaacaact tcatccaccc gaatcaaact tatcatgatg tttgtgagta acttatcatg 1140
tctgcaatgc gtatattacc attctattta aacaaaattt accaacgata tgcttcaaac 1200
aattttttac gtgggtcaaa gctagcatac tgtttttgcg taagttattg tatctgcaat 1260
aaatatatta ccgtgctatt tacctagaag ttattggggg taatgttctt caacgaaaaa 1320
aaattcaggg tcaaaaaatt attgtggtgt ttgaacgaga gttattagct ctccgatggg 1380
taaattatca tgttatttgc acataaatta ccgagggtat gcttttcaac aacttcctcc 1440
atccaggtca aagttatcat ggtgtttttg ctcaagtttt caagtttgtg gtgcgtatat 1500
taccacgatg ttgactgttt acaaggtttt tgagtcgccg actaatcgcc gactaatcgt 1560
cgagtcgtcg cggcggcggc cgccgctaga gaccatga 1598
<210> 149
<211> 958
<212> DNA
<213> 人工序列
<220>
<223> 终止子_PI190945_驯化的_BsaI_构建体
<400> 149
atgaggtctc agctttgcct aaaccgagat gcgcctttgg ttatggagcc caagtcacgt 60
actctgattg gatgctgcag caaatgaagc tacacatttc tattcgggct acctcttttt 120
ttttgaaact ctattcgggc tacctctttg tctcaaagct ggtgtactct tgtgatttgt 180
gttgcgcaac tgatacttct gtgtatgtct gcaagtatca cgaattagtg ggaatgacta 240
ggaataagta agacaagtat cacgaattca gagtcacatg caattttttt cttttctttt 300
gggcataagt agattgccat ttgagtattt tttccattaa tatttgcgta ttcttagatt 360
gggacttaga tatgtaaccc agatttctgt tgttaggaaa cacataccgt gtagcttatc 420
aaaagaaaaa gatctttaga gaaaataaca tcattagatt gttcaacttg gatgaaaaat 480
gtgaaaatgg ccaaaccata attaaataac atcattagat tgttccctgc aaaaaataca 540
tcattagatt gttaaatata attgaacctt ttgttgtctt ttttctaaca tgcacgtggc 600
ggtcaaagat gcgtattaaa agactatgtc agcatgttta ttgtgagtct tggtatgaat 660
attgctctct cctgattcat ccattatgcc tggcaacatt ttctatacaa aaatgtaaat 720
tgaattaaca tgcagacgac tgtcaaagat atactactgt acatcaaaga ccacgtcaat 780
gcgtagacag atttgtgttt acaaactagg cgggtagtgg gaaaggatcc catatgtgtt 840
gaattgttga ttatccactt ttgctaggaa cggatctaca tagtaaaagc cgacccccca 900
agttcgcccc agaagccgtt atttgaaatt aaaatgcggc cgccgctaga gaccatga 958
<210> 150
<211> 4118
<212> DNA
<213> 人工序列
<220>
<223> 编码区_Sr45_基因座_驯化的_BsaI_构建体
<400> 150
atgaggtctc aaatgatggc ggagtttgta gtcaggccac tggtatccac gttgatgaac 60
acggcatcca gctacctgct ggaccagtac aaggtgatgg atggcatgaa ggagcagcgc 120
gaaaccctca agcgccagct gccagccatc ttggacatca tccaagatgc ggagaagaaa 180
ggagcttccc tacctggagt gcgcgcttgg ctcgaagcac tcaagaaggt ggcctatgag 240
gcgaacgatg tctttgatga gttcaagtac gaggccctcc ggcgtgacgc caagaagaag 300
ggacactaca agaagcttgg ctttgacatc gtaagcctct tccctgctca caaccccatt 360
gtatttcgtt acaggatggg caagaagttg tgcaggattg tgcgcaaaat tgagggcctt 420
gtcagagaga tgaatgactt cggattcaac caaactcagc aagcaccacc atccaagcag 480
tggcgcaaca cagattccat aataattgac tctgagaagg atattgtcag caggtccaga 540
aacgaagaga agaagaagat cgttgatata ttgattgatc aagctggcga tagggacctc 600
atcgtccttc ccattgttgg aatgggcggg ctgggcaaga ccacctttgc tcagcttgtc 660
tacaatgacc ctataatcaa ggagcatttc aagctccaga ggtggtgttg tgtgtcagat 720
gattttgatg ttgtgaagat tgcaaacaac atctgtgaga ctaatgagat ccatcgtgaa 780
aaggcattgc agaaccttca gaaagaagta agtgggaaga gatacctcat tgtgttggat 840
gatgtatgga atgaagatgc tgataagtgg gaaaaactga aaacctgcct gaagcacggt 900
gggaagggga gtgcaatatt gacgaccact cgcaatgtgc aagtagcacg aattatgaag 960
atgtgtattg ctgatagcca taatctcaga aatctagata aagtatttct gaaggaaata 1020
tttgagaata gagcattctg tttgcaaaag ccaaaggctg ctgagctaag cgatgtggtt 1080
gataagatta tggacagatg tggaggctct cctttagctg ccaaagcctt tgggtctatg 1140
ttgagtaaca agactagcat gaaagaatgg acagacatac tagccagaag caacacttgc 1200
aatgagggca caaaaacttt tcttgtactc aagctcagct atgatgactt gccatcacac 1260
ttgaaacaat gctttgcttt ttgtgctgta tttcccaaag attatgagat tggtgttgaa 1320
acattaattc agctatggat ggcacatgat ttcataccac tgaaagaagg tgataatctt 1380
gagaaggtag gtagagaaat ttttgatgag ttaacttgga ggtcattctt tcaagatgtc 1440
aagcgaatcc ctcgaagaga gtggtggggg gagctccgtc ctagaacaat atgcaagata 1500
catgatctta tgcatgacat tgccttgtct gttatgggaa aagattgtct tactatagtt 1560
gataggccta atgaaaagga gttactgtcc actggtccta cacgttactt gttctcatca 1620
tatgagtata tcggaactct gttggatgat tatctgaaga aacactctcc cgctctccag 1680
acactgctgt atccgtatcc ctacaccagt gactcagcac cacacttgtc gaagtgcaat 1740
tatctgcgag cactgcaact cttcagtttg agaaaacttc cactctggcc aaggcactta 1800
cagcacctga ggtatcttga tctctcaaat aacatgttga tcgaagagct tcccaaggaa 1860
ataagcatac tgtataatct acagactctt aacctttgta actgcagaag gcttgatcaa 1920
cttccagagg atatgaagta tatggaaaat ctccgtcacc tctatacaaa tggatgctca 1980
tcattgaagt gcatgcctcc aggcctcggg caactcactt ctctacagac tctaacatat 2040
tttgtggtga gctctagtcc tggttgcagt actattagag aactacagga cttgaatctt 2100
ggtggtgagt tagagctatc tcgtctgcag tttgcaactg aagtagatgc taaagcatgc 2160
agccttggaa ataaagagaa acttacacat ttatctctta aatggggtga tgacagcagt 2220
gatgaattgg gccaccacag gaatgtgctc gatgctctta aacctcatgc tgtcctggag 2280
tttctaagaa tacgctccta tagaggtact ggcttccccg catgggtggt aagtattaat 2340
tttctgcagc atttgaccga gctccagcta gatggttgta caatgtgtga ggaatttccc 2400
caattcggtc aatttaagtc ccttgaagtt cttgttttga aaaggttgaa caaacttcaa 2460
agcctatgca atcacagttc atctgcaata tttccagcat taaaagttct cagattaaaa 2520
aaattggaga tttttgagag atgggtggca acagaaggag aagagttagc atttcctcaa 2580
cttgagaatg ttaaaattaa ggactgccca aagctagcaa ttctgcctga agcaccaaaa 2640
ctcaagttta tagctctaaa agaagaaaag gcacagctat ccttatcaat atttaagtcc 2700
aggtatatgg cttgcttgtc tggggttggc ctgtccgtcc gtgatacaga agcagcacca 2760
agaacagagc ttgatcagga ttgtgaagta tctctttcga acctgctgtt ggatggttgc 2820
aactttttgt tctgctcaac tccattgcag ccaactgttg gggtctggaa atggtttggt 2880
cagcttgtgc atttggaaat aaaatcttgt gatatgctca tctactggcc tgaagaagag 2940
ttccgttgct tggtatcatt gaatagttta tccattaatt cctgcagcaa gctagtagga 3000
cacacccagg ggaaaggatg ccgtacccgt actcaagtaa gggatcaact cctgccaaat 3060
cttaaaaatt taagaataca tcattgtgga agtttgacag agctttttgt tctgccgcca 3120
tctctcacaa gtattgatat gctggattgc aatagtattg agtccatact ggggcaggat 3180
gatacagagt tggagagtat actacacttt gatacagcat catcatcgga acacttcaat 3240
gacctcacgt ccacatgttt gctagagcag tcactttcac ccagaattaa tcctttgcca 3300
tgtcttgatt atttgcgtat agttagctgt aagaagcttc gtttcgtgcc agtgcagtta 3360
gatgcactcc aatttttgtg tatttctgat tgcaatgggc tggagtcgct ggattgtttg 3420
ggagatctac cgtcactgga atatctatac cttatgggct gcaaacatct ggcatctgta 3480
ccaggaagtc ttgggagtta ctcggccctt cagaagctcc gcattgaata ctgcccagct 3540
ttaaatatga agccacttca tggacatctc caacagcggc ttgatagcct tgaattgaag 3600
gatctatcta aggctggttc aagccatcct aatgaaggta cctttcaatt tcatttaccc 3660
cattttgtat ctctttttcc atctgtatag tttacagatg tacatattgc agcctgtagt 3720
ttcaagttta tccaactcct tgtattttca catttggtat gtagtgaccg cgtacacagt 3780
ttctctgata tggctattcc aatatcattt atttgtatac cgtaagtttg acttggattg 3840
acgtcgtcat gtgtggccga tctgtatttt ctatcatttt ggtaaaagtt aatacaccat 3900
ggaagtatgc tctcttgtgt agtgaccgta cacagatcat tcttttatgt ctatcccact 3960
atcatttatt tgtaaagttt gacttgctat taatgttcac agagatattc tgacattgtt 4020
ttccatgtac agggcctaaa ctatgggaac caaaatcctg gaaatatatg attccttcac 4080
tgcgcaagcg tgagtctgaa taggcttaga gaccatga 4118
<210> 151
<211> 9744
<212> DNA
<213> 人工序列
<220>
<223> 完整_基因_Sr33-P_Sr22-CR_Sr33-T_天然_构建体
<400> 151
atgagcggcc gctttaagag tgttgaggat gtgcttactt aaatttcaaa agggctattt 60
agggataata tttggtgaag catggctggt ggactgacca taggtaccca ctgaataaac 120
atatcagccc aaaggcagag ggtatccctc cctgtaatac gtactagtat tcgaggagcg 180
tgctaaagta gtatcctctc ttgatgctcc tcgtctccct ccactgagta ctcccttcct 240
ctcctcattc tcatctcagt cctctcctcc tccctcgaat aagatagatc cgcccttgtt 300
gctgatctca tctcgcctcc tattggcctc gcccctgcca actgactgct ctattggtga 360
ccgtggactt tttgggtata tatatttcct gtcgatttgt ttccacttaa agtagcaatg 420
cttggatcta aaacttatta cttctcctgc ttcagattct gatccatttg tgttgattga 480
actcagggtg ggatcgtggg gctctgtatc tatctaggat ggatattcca agcaatttac 540
tctcttgagt gctgttggtc ttcctctgct cctcgtctcc gcttcctctc agtctcctcc 600
attgcgctgc ctggaacaga tcgatccgcc ttgttgctga tctcatccat cctccgtgga 660
gatttcgggt ggcagtttcc agtgactatt cagagcacca tactagtggc ctggtaaaaa 720
tttccctccc atctttcttg ctattctctt cttattaatc tgctgatttg cttgatggta 780
agttcttact tattcttcca gatcgagtga agcgacatcc gccgccgtct tgtcccctac 840
gacgccgctg tgttctgctc tgtcctcctc tgccggtgaa gcatcaacgg tgacggccat 900
ccggtgttga cgccgcggct gtgttctcct ctgccgtgaa gtatcaaagg tgggtttgga 960
tggatatcac agtttggatt ggcaactctg ttctatagct cgttgaaatt aaagcttcac 1020
acagttttga ttggattaca gctccccata ttcatctatt ttgcaccatg catcgaatcc 1080
actgacctcc acacgctcac ggatctggcc tctggtgccc acacgctcac ggatccgaca 1140
gagtgctccc ttcctatcat ctcagtcctc atctcctggt cgatccggct tcttgtttgg 1200
tgagctcgtc caacctccga gctcgcccct gacacctgtg gagtggaggt atatttcttt 1260
ccagctgaag tacgcttgga tctaaaaact tacgtctcct gcttcactgc ttgcttggga 1320
gtctgatcga tttgtgttgg ttgaatcatt tggggtggca gtggagcagc tagcaagaat 1380
catggagttt ccaatcatta ttcagagcac cacgcatgga atccactgac ctggtaactt 1440
tgtctcatct ttcttgctac tgaaatcttc cacatcccca ccttccttgt tcatctgctg 1500
atttcccaga ttatacttcc agattgagtg aagcgccgtc ttgtcccctc acggatctgg 1560
cctctggtgc ccagccgccg ctttcgcttc gttgtccgac gaggccaccg ccgctgtgtt 1620
ctgctctgcc gtgaagcaca aaaaggtggg gtcttcctct gctctttctc cactgacggc 1680
gatccgacct tgacacccgt ggccgtggca gtggggtctt cctctgctct gtttttttac 1740
cagttattgg atctaaaaag ggatctaaaa gttattactt ctcctgcttg agattctgac 1800
cgatttgtgt tgattgaatc cagggtgggg tcgtggggct ctgtatctat ctagtacgga 1860
gtaccactgt attccaagca atttactctc ttgactgctg ttggacttcc tctgtccccg 1920
cttccattgt gctgccttga acagatccat ccaccttgtt gctaatctca tccaacctcc 1980
gggtatgttt cttgtcgatt tgtttccagt aaagtagcaa tgcttgggtc taaaaagtca 2040
ttgcttctcc tgcttgacga gtctcattga tttgcccagt cattattcag agcaccatgc 2100
atccaaggcg ctgacccggc tataagtttt tattacaggg ccgccgtgtt gtccccatcg 2160
agcaaagcgt ttaatccact gacccgcttc gttgtccgca cagcgtgacc atccgacgaa 2220
gacgccgccg ttgtgttctg ctctcctctg aagcatcaaa aggtgggctt ggtccagatc 2280
cccacattca tcgatttggc ttgccaactc agttccacca gagcttggat tgtgttacag 2340
atccctaaat tcatcgattt gcaggtttca gctgattgat caagagagct ctcatggcgg 2400
aagttctggt gagcgcatcc acaggggcaa tgggttccct cctgcggaag ttgggagcta 2460
tgttgacgga tgaatacaag ctgctcaaga acgtccgtgg ggacatcaag ttcctcaagg 2520
acgagctgga ggtcatgtgc gcgttcctcc tcaagatgtc agatgtggag gagcctgacg 2580
aaccaacaaa gctgcgtgtg acggcggtgc gggagatgtc ctataagata gaggacaaca 2640
tcgacaagtt catggtcctc gtggaacagg aacacgggtc ctcctgttcc gaggcggctc 2700
atggcgtcgc caagctcatg gacaagtgca agaacttgct gccggacatc aaggcccgcc 2760
gcaggatcgc caaggaggtc aaagacatca agaaagaaat taaggatgtc agcgacagat 2820
tttcaaggta ccagatcttg ttctccttac agtactatta gtacctacca tcagatttgt 2880
gaccgatttg ttgccactca aggtacaaga ttgacgactc ctcctcctct atgccggcaa 2940
aagacaaggt cgaccctcga cttcgtgcag tctacaaaga cgccgcagag ctcgtcggaa 3000
ttgatggacc aaaggatgaa cttgtcaagt ggctgaatga gaaagaaggc caatcactga 3060
aatctgtctc tattgttgga tatggagggt tgggcaagac cacgctcgcc aaccagatcc 3120
gggtcaacct tggagcgacc ttcgactgcg gggcttttgt ctcgatttca cgcaagcctg 3180
acatgaaggc gatattaaga tctatattat cacaaatcac caagaaagac gatgcttgct 3240
ctagattaga tgatatacaa ctcatcatcg acaagattcg agaattcctc caagacacaa 3300
ggtatgtatg tgagatctgc tacgaagtaa cattgcttaa ttagctcatg cataatcggt 3360
tgcctagaat tttgctaatg aagattgctt tgtgtgattt actttcgtcc tcaacttgta 3420
tgactgcgct taaactttca agtcattgtt tcttgcaact tatgtcgatc tgttcactaa 3480
ttaagatata gctaaaatgt gtttgccgat ttctgtcgcc acacattttc taatttgatt 3540
gccacaagtt tgttaaactt gcggctatgt tctgtttatt aaataattgt gacatggcgt 3600
cagtcaaaac aataaagtac atgcaattcc ctctcttttg tacaagataa atatccgcac 3660
gcactacatg actgcaaata tatctatctt ctaaggaccc aaagcacttg catgtgcaga 3720
ccattgttac cggatatgca gtacattagg tttgtgatta gccactttaa gaacataggg 3780
tttctagagg ctataaacct acggttatca gtattataat cttgaatgga tatcaaacat 3840
ccgttgaaga atctatgccc ataacacaaa aagagaaaag ggaagaatta atcatagtca 3900
gatcggacca gcctgactag cgtaacataa tgacaacagt aggttgttgc gtctttcctg 3960
gtttcgtagc aatttgcatg acgaattgag gcgcagagcc accactcaat atgtggtcct 4020
accaatcatg aggaaataac ttgttaccaa aatgacaaaa gaaaataggt agtccccatc 4080
attgtttcaa tatctatcct cttgcgaaca tgctattggc agtggcaaaa cattgtacct 4140
tgccactggc aacagctgcc tttactcggc ctgagcattc atattttttt cctcttggtt 4200
gttaaatctt gctatttttc gcttgccttc tatggcaatc tttttttttc ttatattgcc 4260
accaaattgt atctaagagc aactccaaca cgccgacgca atttttgtcc atttagatcg 4320
gccaagcgga cggggtgtct ggttttgcct ttgggtggtg cgcctacgca ttagtccgcg 4380
tgggcattta aaaaacttaa aaagcttaaa ataattaaat ataaataaaa atgcatatta 4440
aagcccgtca aagtccacat aattaaacat aatacattaa tatattaata aaaaacagcc 4500
gccccagccc gcagccctag gcgtcttcgt cggaggagtg ggtgaggtcg aagaatgccg 4560
gcagctcagc ccacgggaag gcctgctggt gggcagccag caccgcctgc tccgctgtct 4620
gcggcggcgg cggccctacc tggcaccgcg cacgcctcct cctcgcgctc ctgccgctcc 4680
tcctcctcat cgcacgccca acgaagtttt ggcgctcctc cccctcgggt tcccgctgca 4740
tgcggacctg gccgtcgtac cgctcctcct ccagcgtgcg gtccttccac gccacgaggt 4800
aggccgcctc ctcctccatc acgcacagcc aaatgggcgg cttgctcatc cgcttgcaga 4860
cgatgccctg ccattggtaa acgccggtgg gcgcgggtgg tgctagcgct ggtgcaggcg 4920
gtgacgatgt ggctggtggt cccggcaggt agacactata gctggcggcc gatagctgca 4980
gcgtgtcatc gaggccagac cactaggagt cctcgcacta agactcctcg gtgacgtgat 5040
gggccccctc ctccaatgcc ttgaggaacg ccgcctcctc ctcaacctac tatttgacca 5100
ccggtggcgg cggtggaggg caggcttcga gctggacggc gtcgcggagg cgcacatggt 5160
gctcgatggt gaaccagact tctcagttag gggagtctgg cctcgatggg tggagtcgcg 5220
acgctgctct ggcgtcagga gagcacgccg ggcccgaatc tcgttggcgc gcgcacgctc 5280
ggactgtggc accgccggca ctggaatgtg gtgggggtcg aggtgccagc cgtgggggaa 5340
gttcacgtcc ggccacggca gtgagacgcg gtactcccgg tggtactacg cgtggtgcac 5400
cgggatgtac tggtggtccc gtggcgctgc cgccctccca tcccgcagcg ccgacaccaa 5460
tgcgaacccc ttcgctgccg gcctcaccgg cccttgcaac gagccggcct cgtggtcgtg 5520
cttgccttgc ccttaccttt gccaaagaaa cccatgacgg ctagggtttt ctgtcaccgg 5580
cgagggagca gatgtggact agagtgggag gagaagtgga tgaggcccgc cccgccgcac 5640
ccttgcaatt aaaaagggcg tgatccggac gtttctagtc actgccatgc gggcccgagg 5700
taggtgggcg ccattgacta cgtctgtggt ggtagttggc tggcagacac gccagcccga 5760
ggcgcacacc gagcggacgc gcaacacgtc cgtctcgtgt tcgtgtgaac gcaaatcagg 5820
cacaaatttg ggctggaaat gcgtctacat ggatgccgaa cggacgtgat ttgggtttgt 5880
gtccgcacgt tgggccggcg tttttgtgcc tttcgaccct aatggacaca cacggaccaa 5940
atccgttggc gtgttagagt tgcttgtacc tagtactact atgtgtggag ttgttatctt 6000
tgagtttctc ccccatctgt agctgtgtat tattgtgaga tgctctcagg atttcactta 6060
ttttagataa tgacaacttt cttgatgcta gacaattaca tcaaggtgta ttatatctag 6120
gatgcacaca gatgtgagat tgtttcagga ttttaggtta cttggtaggt gttactataa 6180
atatctaatg agctctaatg tgtatgttca tgaaactgtg ttgcaggtac tttatcataa 6240
ttgatgatat atgggagtta ggaacatggg aaactttgaa atgtgcattt gtcaagaata 6300
cattgggcag cagaataatt atcacaacac gtatagttga tgttgccaaa tcttgttctc 6360
cttctagtga agatcttgtc tatgagatga aaccactcag tgaggctgac tcgaagaaat 6420
tgtttttcaa gaggatattt ggctgtgaag aaagctgtcc tgatagcttg aaagaagctg 6480
ccaatgatat tttgaaaaaa tgccgtggcc tacctctggc catcaatgcc atttctagcg 6540
tgttggtcac aacaagggaa acaaaagaag agtgggatcg ggtgcgacat tcaatacgct 6600
cttcaaaggt caaaagtgat ataatagaga ccatgaatta catattgtct cttagttatt 6660
ttgatcttcc ccaccaccta agaagctgct tattgtacct ggctctgttt cctgaagacc 6720
aattgattgg aaggaagcgg ttagtacgca gatggatttc tgaaggcttt atccatggag 6780
aaagtggaca agatctcatg gaattaggag aggaatattt tcaccagctt gtgaacagaa 6840
gtctaataca gcccggcaac atagggtatg acggcaaggc aatgtattgc cgagtccatg 6900
acaccatcct tgatttccta attgacaaat cctctgaaga gaacatgtgt actgtcctaa 6960
agaaacaatg caagcctaat ggcatagttc gtcggctctc tctaatgggg aatgaggatg 7020
aagaaatcgt ggagcaattg gatctgtcac acgctcggtc aatcactgct tttggggata 7080
tcaagctatt gccttcgctt gggaggtcaa aatgcttgcg cgtgctagac ttgcaagact 7140
gcgatcagtt ggaaaatcat catatcaagg atattgaaag actctatcag ctaagatact 7200
tggatatctc ttctacagga atcacggagc ttcccaggca aattggggag ttgctgtatc 7260
tagagacgct agttgcctat ggattacgtg agcttcctga aagcacaagt cggctacaac 7320
gactggcacg tttgtttgtt tactctggtt gtaagcttcc aggtgggttg ggaaacttga 7380
taaacttgca agagcttgac tgcgttgatg ccttgcattt gaagcatgtg gaagagctcg 7440
gcaaactaac aaatctgaga aagctgagta ttaaattgga cactggtggg attgaaggca 7500
acaaattaga agagtccaag gaaaagctgg tgtcctctct ctgtaaactg gacgaatgcg 7560
gcctcctttc cctgagtatt gattattatc tgagagaaaa ggatggagaa gagccgttcc 7620
tccctgcttt gggttgtatc caggaggttt ttgtatatgg acaagatatc agccggatta 7680
gcagatggct cgcgtcactt cccaacctac acaggttgct tttggacgat ccgaagatag 7740
agcagcagga tattgagatg attggattga tacccaatct gatcgatttg acactgccgc 7800
ctctatacaa aacagacgac gctgggcggc tcatcatcag gcgtgaagga tttcaacagt 7860
tgcagaagtt tgaggcttac aatactcgta tgggggtttt aatgtttgaa ccgggggcta 7920
tgccgaggct caaggagctt aaactccata acttcatcga aaagcccaaa tctgctgcgg 7980
tcgatttcga ctttggcatc caacgcctct ccagccttgc tcgcctcact gtcagcttat 8040
cctgcggtgg ctggacggtg gcagaagtag aggctgcgga ggatgctttc aagagcatgg 8100
ccgaggcgaa ccccaaccgt ccaatactgg agatgacaag atataatacg cagcatatgt 8160
tacaagacga gcaaataggt atgacaggct caggtactat taactagttt gtacttttgt 8220
cttggtgagc agtagatcaa aagatgtgca tatgcttcaa tcttttttcc ggtaatctat 8280
ttcagctaca acgccggccg tcaagtccta gctgacgttg cttcaggtgt gctctcaatc 8340
tatacatatt tactcatcat attattctcc accttttttt ccatctctag agctcagctt 8400
gtcattgcat tgttcaattg tgcttctcta gcagctacgg aaattctgaa caggcaaagg 8460
taattttaca gaagtttctt tggcataagt atctgagaaa aatgcacttg gcacaattta 8520
ccttttcgca gtagaaaaga gtcagtggat cataccttct taattgttcg attgctcgta 8580
atacttgggg gcactctatg attgctgtgt gcttttagtt ttgatatagt tcctgttagc 8640
atggacagac tgtgtgattg gttcatgaat ttcagggtgg ttctaatgag gaaatgatgc 8700
ttgcttcaga aatagtttcc atttgtacct aagctgtgta gttggtaaag ttgcattttg 8760
gatgttctgt tggtatagct tacagaagaa agacttgcgt tgtgcaggta ccaggggcgc 8820
aggttactgc tagtggcgtc tgagatgttt cataaacgtt gggtgggcac cggtcatgca 8880
aaaaattgaa ggataaaaag gttccgagag gttgtgtgat gtgctccttt taaatggcag 8940
tcgcttgcat aaagagatgt tgcttggttt tttagtaaag cgttgtagta agttgctctg 9000
tttcggctga gagccatcgt tgttgctgtc tgttacatcg aatggctggc tcatgtcatg 9060
ttgatgtttt ttttatctga accggacaaa cctgagggtg tagtatcatg tttgtaatga 9120
ctgaaattct gggctcaccc gagctcctta attcgtaaac aactgcgact cattgttgga 9180
ttccttactt cagaaaatat ttacttgctc tggctcatac agctctgcat gttttgtagt 9240
ggtttgtcgc tgtttaggtg ctgcttcaga ctcctggtac ctttggccgc tgctgctact 9300
gcatgagctt tgtatggcat tgttagtata gtttggtgtc attgggtttt cgccccatgg 9360
tgagtgtcca ttgcgcgcac tcgcagcggt ttcctggcga taagttgaac ttgatctttc 9420
tctttcccac ctcctaaacg tctattcaga cttgtgtata tttgcattac ggggattacc 9480
cgagttcaaa aataaatatt atttactagt tctgccaatg tgtttccgtt acatacaaat 9540
aaaagggtgc ttccagctac aagtatattt ttgagaaggg ccattgcaaa acgaaatgaa 9600
gcccaaattc taccaacatg tttctgtcac atatacatta aaaaaacggt gtaaatgtcc 9660
cgtcaaaaaa gggtgtatgt aggaaaagga gttcgacgcc gccagctccc tcctaacgct 9720
cagattgtct tcgcggccgc atga 9744
<210> 152
<211> 21636
<212> DNA
<213> 人工序列
<220>
<223> 转化构建体
<400> 152
gtcgacgatc gtcaacgttc acttctaaag aaatagcgcc actcagcttc ctcagcggct 60
ttatccagcg atttcctatt atgtcggcat agttctcaag atcgacagcc tgtcacggtt 120
aagcgagaaa tgaataagaa ggctgataat tcggatctct gcgagggaga tgatatttga 180
tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc 240
cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca 300
aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg 360
tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca 420
ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt 480
ttttaatgta ctgaattcca atccccaaaa atctgagctt aacagcacag ttgctcctct 540
cagagcagaa tcgggtattc aacaccctca tatcaactac tacgttgtgt ataacggtcc 600
acatgccggt atatacgatg actggggttg tacaaaggcg gcaacaaacg gcgttcccgg 660
agttgcacac aagaaatttg ccactattac agaggcaaga gcagcagctg acgcgtacac 720
aacaagtcag caaacagaca ggttgaactt catccccaaa ggagaagctc aactcaagcc 780
caagagcttt gctaaggccc taacaagccc accaaagcaa aaagcccact ggctcacgct 840
aggaaccaaa aggcccagca gtgatccagc cccaaaagag atctcctttg ccccggagat 900
tacaatggac gatttcctct atctttacga tctaggaagg aagttcgaag gtgaaggtga 960
cgacactatg ttcaccactg ataatgagaa ggttagcctc ttcaatttca gaaagaatgc 1020
tgacccacag atggttagag aggcctacgc agcaggtctc atcaagacga tctacccgag 1080
taacaatctc caggagatca aataccttcc caagaaggtt aaagatgcag tcaaaagatt 1140
caggactaat tgcatcaaga acacagagaa agacatattt ctcaagatca gaagtactat 1200
tccagtatgg acgattcaag gcttgcttca taaaccaagg caagtaatag agattggagt 1260
ctctaaaaag gtagttccta ctgaatctaa ggccatgcat ggagtctaag attcaaatcg 1320
aggatctaac agaactcgcc gtgaagactg gcgaacagtt catacagagt cttttacgac 1380
tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca cgacactctg gtctactcca 1440
aaaatgtcaa agatacagtc tcagaagacc aaagggctat tgagactttt caacaaagga 1500
taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc atcgaaagga 1560
cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggctatca 1620
ttcaagatct ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg 1680
tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gacatctcca 1740
ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc tctatataag 1800
gaagttcatt tcatttggag aggacacgct cgagtataag agctctattt ttacaacaat 1860
taccaacaac aacaaacaac aaacaacatt acaattacta tttacaatta ccatgagccc 1920
agaacgacgc ccggccgaca tccgccgtgc caccgaggcg gacatgccgg cggtctgcac 1980
catcgtcaac cactacatcg agacaagcac ggtcaacttc cgtaccgagc cgcaggaacc 2040
gcaggagtgg acggacgacc tcgtccgtct gcgggagcgc tatccctggc tcgtcgccga 2100
ggtggacggc gaggtcgccg gcatcgccta cgcgggcccc tggaaggcac gcaacgccta 2160
cgactggacg gccgagtcaa ccgtgtacgt ctccccccgc caccagcgga cgggactggg 2220
ctccacgctc tacacccacc tgctgaagtc cctggaggca cagggcttca agagcgtggt 2280
cgctgtcatc gggctgccca acgacccgag cgtgcgcatg cacgaggcgc tcggatatgc 2340
cccccgcggc atgctgcggg cggccggctt caagcacggg aactggcatg acgtgggttt 2400
ctggcagctg gacttcagcc tgccggtacc gccccgtccg gtcctgcccg tcaccgagat 2460
ttgatgaccc gggggatcct agagtcctgc tttaatgaga tatgcgagac gcctatgatc 2520
gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct 2580
cagatcctta ccgccggttt cggttcattc taatgaatat atcacccgtt actatcgtat 2640
ttttatgaat aatattctcc gttcaattta ctgattgtac cctactactt atatgtacaa 2700
tattaaaatg aaaacaatat attgtgctga ataggtttat agcgacatct atgatagagc 2760
gccacaataa caaacaattg cgttttatta ttacaaatcc aattttaaaa aaagcggcag 2820
aaccggtcaa acctaaaaga ctgattacat aaatcttatt caaatttcaa aagtgcccca 2880
ggggctagta tctacgacac accgagcggc gaactaataa cgctcactga agggaactcc 2940
ggttccccgc cggcgcgcat gggtgagatt ccttgaagtt gagtattggc cgtccgctct 3000
accgaaagtt acgggcacca ttcaacccgg tccagcacgg cggccgggta accgacttgc 3060
tgccccgaga attatgcagc atttttttgg tgtatgtggg ccccaaatga agtgcaggtc 3120
aaaccttgac agtgacgaca aatcgttggg cgggtccagg gcgaattttg cgacaacatg 3180
tcgaggctca gcaggacctg caggcatgca agctctagag cggccgcgtg cagcgtgacc 3240
cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa aaaattacca 3300
catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata catatattta 3360
aactttactc tacgaataat ataatctata gtactacaat aatatcagtg ttttagagaa 3420
tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg acaacaggac 3480
tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa atagcttcac 3540
ctatataata cttcatccat tttattagta catccattta gggtttaggg ttaatggttt 3600
ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta aattaagaaa 3660
actaaaactc tattttagtt tttttattta ataatttaga tataaaatag aataaaataa 3720
agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg aaacattttt 3780
cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta acggacacca 3840
accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg catctctgtc 3900
gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc cgctgtcggc 3960
atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg gcctcctcct 4020
cctctcacgg cacggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc 4080
ctcgcccgcc gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttgt 4140
tcggagcgca cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt 4200
caaggtacgc cgctcgtcct cccccccccc ccctctctac cttctctaga tcggcgttcc 4260
ggtccatggt tagggcccgg tagttctact tctgttcatg tttgtgttag atccgtgttt 4320
gtgttagatc cgtgctgcta gcgttcgtac acggatgcga cctgtacgtc agacacgttc 4380
tgattgctaa cttgccagtg tttctctttg gggaatcctg ggatggctct agccgttccg 4440
cagacgggat cgatttcatg attttttttg tttcgttgca tagggtttgg tttgcccttt 4500
tcctttattt caatatatgc cgtgcacttg tttgtcgggt catcttttca tgcttttttt 4560
tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt ctagatcgga gtagaattct 4620
gtttcaaact acctggtgga tttattaatt ttggatctgt atgtgtgtgc catacatatt 4680
catagttacg aattgaagat gatggatgga aatatcgatc taggataggt atacatgttg 4740
atgcgggttt tactgatgca tatacagaga tgctttttgt tcgcttggtt gtgatgatgt 4800
ggtgtggttg ggcggtcgtt cattcgttct agatcggagt agaatactgt ttcaaactac 4860
ctggtgtatt tattaatttt ggaactgtat gtgtgtgtca tacatcttca tagttacgag 4920
tttaagatgg atggaaatat cgatctagga taggtataca tgttgatgtg ggttttactg 4980
atgcatatac atgatggcat atgcagcatc tattcatatg ctctaacctt gagtacctat 5040
ctattataat aaacaagtat gttttataat tattttgatc ttgatatact tggatgatgg 5100
catatgcagc agctatatgt ggattttttt agccctgcct tcatacgcta tttatttgct 5160
tggtactgtt tcttttgtcg atgctcaccc tgttgtttgg tgttacttct gcaggaatga 5220
tggcggaagt tctggtgagc gcatccacag gggcaatggg ttccctcctg cggaagttgg 5280
gagctatgtt gacggatgaa tacaagctgc tcaagaacgt ccgtggggac atcaagttcc 5340
tcaaggacga gctggaggtc atgtgcgcgt tcctcctcaa gatgtcagat gtggaggagc 5400
ctgacgaacc aacaaagctg cgtgtgacgg cggtgcggga gatgtcctat aagatagagg 5460
acaacatcga caagttcatg gtcctcgtgg aacaggaaca cgggtcctcc tgttccgagg 5520
cggctcatgg cgtcgccaag ctcatggaca agtgcaagaa cttgctgccg gacatcaagg 5580
cccgccgcag gatcgccaag gaggtcaaag acatcaagaa agaaattaag gatgtcagcg 5640
acagattttc aaggtaccag atcttgttct ccttacagta ctattagtac ctaccatcag 5700
atttgtgacc gatttgttgc cactcaaggt acaagattga cgactcctcc tcctctatgc 5760
cggcaaaaga caaggtcgac cctcgacttc gtgcagtcta caaagacgcc gcagagctcg 5820
tcggaattga tggaccaaag gatgaacttg tcaagtggct gaatgagaaa gaaggccaat 5880
cactgaaatc tgtctctatt gttggatatg gagggttggg caagaccacg ctcgccaacc 5940
agatccgggt caaccttgga gcgaccttcg actgcggggc ttttgtctcg atttcacgca 6000
agcctgacat gaaggcgata ttaagatcta tattatcaca aatcaccaag aaagacgatg 6060
cttgctctag attagatgat atacaactca tcatcgacaa gattcgagaa ttcctccaag 6120
acacaaggta tgtatgtgag atctgctacg aagtaacatt gcttaattag ctcatgcata 6180
atcggttgcc tagaattttg ctaatgaaga ttgctttgtg tgatttactt tcgtcctcaa 6240
cttgtatgac tgcgcttaaa ctttcaagtc attgtttctt gcaacttatg tcgatctgtt 6300
cactaattaa gatatagcta aaatgtgttt gccgatttct gtcgccacac attttctaat 6360
ttgattgcca caagtttgtt aaacttgcgg ctatgttctg tttattaaat aattgtgaca 6420
tggcgtcagt caaaacaata aagtacatgc aattccctct cttttgtaca agataaatat 6480
ccgcacgcac tacatgactg caaatatatc tatcttctaa ggacccaaag cacttgcatg 6540
tgcagaccat tgttaccgga tatgcagtac attaggtttg tgattagcca ctttaagaac 6600
atagggtttc tagaggctat aaacctacgg ttatcagtat tataatcttg aatggatatc 6660
aaacatccgt tgaagaatct atgcccataa cacaaaaaga gaaaagggaa gaattaatca 6720
tagtcagatc ggaccagcct gactagcgta acataatgac aacagtaggt tgttgcgtct 6780
ttcctggttt cgtagcaatt tgcatgacga attgaggcgc agagccacca ctcaatatgt 6840
ggtcctacca atcatgagga aataacttgt taccaaaatg acaaaagaaa ataggtagtc 6900
cccatcattg tttcaatatc tatcctcttg cgaacatgct attggcagtg gcaaaacatt 6960
gtaccttgcc actggcaaca gctgccttta ctcggcctga gcattcatat ttttttcctc 7020
ttggttgtta aatcttgcta tttttcgctt gccttctatg gcaatctttt tttttcttat 7080
attgccacca aattgtatct aagagcaact ccaacacgcc gacgcaattt ttgtccattt 7140
agatcggcca agcggacggg gtgtctggtt ttgcctttgg gtggtgcgcc tacgcattag 7200
tccgcgtggg catttaaaaa acttaaaaag cttaaaataa ttaaatataa ataaaaatgc 7260
atattaaagc ccgtcaaagt ccacataatt aaacataata cattaatata ttaataaaaa 7320
acagccgccc cagcccgcag ccctaggcgt cttcgtcgga ggagtgggtg aggtcgaaga 7380
atgccggcag ctcagcccac gggaaggcct gctggtgggc agccagcacc gcctgctccg 7440
ctgtctgcgg cggcggcggc cctacctggc accgcgcacg cctcctcctc gcgctcctgc 7500
cgctcctcct cctcatcgca cgcccaacga agttttggcg ctcctccccc tcgggttccc 7560
gctgcatgcg gacctggccg tcgtaccgct cctcctccag cgtgcggtcc ttccacgcca 7620
cgaggtaggc cgcctcctcc tccatcacgc acagccaaat gggcggcttg ctcatccgct 7680
tgcagacgat gccctgccat tggtaaacgc cggtgggcgc gggtggtgct agcgctggtg 7740
caggcggtga cgatgtggct ggtggtcccg gcaggtagac actatagctg gcggccgata 7800
gctgcagcgt gtcatcgagg ccagaccact aggagtcctc gcactaagac tcctcggtga 7860
cgtgatgggc cccctcctcc aatgccttga ggaacgccgc ctcctcctca acctactatt 7920
tgaccaccgg tggcggcggt ggagggcagg cttcgagctg gacggcgtcg cggaggcgca 7980
catggtgctc gatggtgaac cagacttctc agttagggga gtctggcctc gatgggtgga 8040
gtcgcgacgc tgctctggcg tcaggagagc acgccgggcc cgaatctcgt tggcgcgcgc 8100
acgctcggac tgtggcaccg ccggcactgg aatgtggtgg gggtcgaggt gccagccgtg 8160
ggggaagttc acgtccggcc acggcagtga gacgcggtac tcccggtggt actaactacg 8220
cgtggtgcac cgggatgtac tggtggtccc gtggcgctgc cgccctccca tcccgcagcg 8280
ccgacaccaa tgcgaacccc ttcgctgccg gcctcaccgg cccttgcaac gagccggcct 8340
cgtggtcgtg cttgccttgc ccttaccttt gccaaagaaa cccatgacgg ctagggtttt 8400
ctgtcaccgg cgagggagca gatgtggact agagtgggag gagaagtgga tgaggcccgc 8460
cccgccgcac ccttgcaatt aaaaagggcg tgatccggac gtttctagtc actgccatgc 8520
gggcccgagg taggtgggcg ccattgacta cgtctgtggt ggtagttggc tggcagacac 8580
gccagcccga ggcgcacacc gagcggacgc gcaacacgtc cgtctcgtgt tcgtgtgaac 8640
gcaaatcagg cacaaatttg ggctggaaat gcgtctacat ggatgccgaa cggacgtgat 8700
ttgggtttgt gtccgcacgt tgggccggcg tttttgtgcc tttcgaccct aatggacaca 8760
cacggaccaa atccgttggc gtgttagagt tgcttgtacc tagtactact atgtgtggag 8820
ttgttatctt tgagtttctc ccccatctgt agctgtgtat tattgtgaga tgctctcagg 8880
atttcactta ttttagataa tgacaacttt cttgatgcta gacaattaca tcaaggtgta 8940
ttatatctag gatgcacaca gatgtgagat tgtttcagga ttttaggtta cttggtaggt 9000
gttactataa atatctaatg agctctaatg tgtatgttca tgaaactgtg ttgcaggtac 9060
tttatcataa ttgatgatat atgggagtta ggaacatggg aaactttgaa atgtgcattt 9120
gtcaagaata cattgggcag cagaataatt atcacaacac gtatagttga tgttgccaaa 9180
tcttgttctc cttctagtga agatcttgtc tatgagatga aaccactcag tgaggctgac 9240
tcgaagaaat tgtttttcaa gaggatattt ggctgtgaag aaagctgtcc tgatagcttg 9300
aaagaagctg ccaatgatat tttgaaaaaa tgccgtggcc tacctctggc catcaatgcc 9360
atttctagcg tgttggtcac aacaagggaa acaaaagaag agtgggatcg ggtgcgacat 9420
tcaatacgct cttcaaaggt caaaagtgat ataatagaaa ccatgaatta catattgtct 9480
cttagttatt ttgatcttcc ccaccaccta agaagctgct tattgtacct ggctctgttt 9540
cctgaagacc aattgattgg aaggaagcgg ttagtacgca gatggatttc tgaaggcttt 9600
atccatggag aaagtggaca agatctcatg gaattaggag aggaatattt tcaccagctt 9660
gtgaacagaa gtctaataca gcccggcaac atagggtatg acggcaaggc aatgtattgc 9720
cgagtccatg acaccatcct tgatttccta attgacaaat cctctgaaga gaacatgtgt 9780
actgtcctaa agaaacaatg caagcctaat ggcatagttc gtcggctctc tctaatgggg 9840
aatgaggatg aagaaatcgt ggagcaattg gatctgtcac acgctcggtc aatcactgct 9900
tttggggata tcaagctatt gccttcgctt gggaggtcaa aatgcttgcg cgtgctagac 9960
ttgcaagact gcgatcagtt ggaaaatcat catatcaagg atattgaaag actctatcag 10020
ctaagatact tggatatctc ttctacagga atcacggagc ttcccaggca aattggggag 10080
ttgctgtatc tagagacgct agttgcctat ggattacgtg agcttcctga aagcacaagt 10140
cggctacaac gactggcacg tttgtttgtt tactctggtt gtaagcttcc aggtgggttg 10200
ggaaacttga taaacttgca agagcttgac tgcgttgatg ccttgcattt gaagcatgtg 10260
gaagagctcg gcaaactaac aaatctgaga aagctgagta ttaaattgga cactggtggg 10320
attgaaggca acaaattaga agagtccaag gaaaagctgg tgtcctctct ctgtaaactg 10380
gacgaatgcg gcctcctttc cctgagtatt gattattatc tgagagaaaa ggatggagaa 10440
gagccgttcc tccctgcttt gggttgtatc caggaggttt ttgtatatgg acaagatatc 10500
agccggatta gcagatggct cgcgtcactt cccaacctac acaggttgct tttggacgat 10560
ccgaagatag agcagcagga tattgagatg attggattga tacccaatct gatcgatttg 10620
acactgccgc ctctatacaa aacagacgac gctgggcggc tcatcatcag gcgtgaagga 10680
tttcaacagt tgcagaagtt tgaggcttac aatactcgta tgggggtttt aatgtttgaa 10740
ccgggggcta tgccgaggct caaggagctt aaactccata acttcatcga aaagcccaaa 10800
tctgctgcgg tcgatttcga ctttggcatc caacgcctct ccagccttgc tcgcctcact 10860
gtcagcttat cctgcggtgg ctggacggtg gcagaagtag aggctgcgga ggatgctttc 10920
aagagcatgg ccgaggcgaa ccccaaccgt ccaatactgg agatgacaag atataatacg 10980
cagcatatgt tacaagacga gcaaataggt atgacaggct caggtactat taactagttt 11040
gtacttttgt cttggtgagc agtagatcaa aagatgtgca tatgcttcaa tcttttttcc 11100
ggtaatctat ttcagctaca acgccggccg tcaagtccta ggctttgcct aaaccgagat 11160
gcgcctttgg ttatggagcc caagtcacgt actcggattg gatgctgcag caaatgatac 11220
ttctgtacat gtctgcaagt atcacgaatt agtggaatga ctaggaataa gtaagacaag 11280
tatcacgaat tcagagtcgg atgcaatttt tttcttttct tttgggcata agtagattgt 11340
catttgagta tttttttcca ttaatatttg tgtattctta gattgagact ttgatatgta 11400
acccagattt ctgttgttag gaaacacata ccgtgtagct tatcagaaga aaaagatcta 11460
gcgtgcatct atattctatc ggactatgat atttggcaca tgtgtgccat ataaacaaaa 11520
ctgtcacacc tctatttcct tcactctaaa tagatgtata cattagtttg attctttctt 11580
ctttcacgtg catttctcct tctaattaga tgcatacatt agaggacaaa aaagacgatg 11640
tcattctaga ttttctttat taaaatattt aaaatgtttt gtagctcaaa ccgtcggtcc 11700
gatgtaaaaa ccgtttttac atatacgatt cgtcgcgatg agatctttga aactagatcc 11760
tatgttgaca tgtttcgatg acttttttta accaaaattt accatgcctt ttatatgcaa 11820
gttaccgtgc tatttccaca taagtcatcg gaggcataaa aatggttcac cgacactaca 11880
gttcatatat taccatgatg tttacatata agttaccggg aggggggggg ggggtgtctg 11940
ttttcaataa tttccccacc gagtcaaata ttaccatagt atatgtacgt aagttatcag 12000
gtatccgatg ggtatattac catggctatc ttttaaaaaa atctctaatt taccgcggtg 12060
tttgtatcta aattatcaag tttgtggtgc ctaatttact atgctattta catacaaaag 12120
ttaccagtgt attttttgac aattttttac tattcttccc caaatcaaga ctattaccgt 12180
gctgtttaca cacaagttac cgggtatatt ttcaacaact tcatccaccc gaatcaaact 12240
tatcatgatg tttgtgagta acttatcatg tctgcaatgc gtatattacc attctattta 12300
aacaaaattt accaacgata tgcttcaaac aattttttac gtgggtcaaa gctagcatac 12360
tgtttttgcg taagttattg tatctgcaat aaatatatta ccgtgctatt tacctagaag 12420
ttattggggg taatgttctt caacgaaaaa aaattcaggg tcaaaaaatt attgtggtgt 12480
ttgaacgaga gttattagct ctccgatggg taaattatca tgttatttgc acataaatta 12540
ccgagggtat gcttttcaac aacttcctcc atccaggtca aagttatcat ggtgtttttg 12600
ctcaagtttt caagtttgtg gtgcgtatat taccacgatg ttgactgttt acaaggtttt 12660
tgagtcgccg actaatcgcc gactaatcgt cgagtcgtcg cggcggcggc cgccaccgcg 12720
gctgagtggc tccttcaagc ttgcggatcc gggcggaaat aggtaaagaa gttgcggata 12780
aggtaattgc cattgcagat tatttggatt gagagtgaat atgagactct aattggatac 12840
cgaggggaat ttatggaacg tcagtggagc atttttgaca agaaatattt gctagctgat 12900
agtgacctta ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt gcttagctca 12960
ttaaactcca gaaacccgcg gctgagtggc tccttcaacg ttgcggttct gtcagttcca 13020
aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc ccttaattct 13080
ccgctcatga tcagattgtc gtttcccgcc ttcagtttaa actatcagtg tttgacagga 13140
tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa 13200
gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc 13260
ctcgggagtg cttggcattc cgtgcgataa tgacttctgt tcaaccaccc aaacgtcgga 13320
aagcctgacg acggagcagc attccaaaaa gatcccttgg ctcgtctggg tcggctagaa 13380
ggtcgagtgg gctgctgtgg cttgatccct caacgcggtc gcggacgtag cgcagcgccg 13440
aaaaatcctc gatcgcaaat ccgacgctgt cgaaaagcgt gatctgcttg tcgctctttc 13500
ggccgacgtc ctggccagtc atcacgcgcc aaagttccgt cacaggatga tctggcgcga 13560
gttgctggat ctcgccttca atccgggtct gtggcgggaa ctccacgaaa atatccgaac 13620
gcagcaagat cgtcgacccc tttccgacgc tcaccgggct ggttgccctc gccgctgggc 13680
tggcggccgt ctatggccct gcaaacgcgc cagaaacgcc gtcgaagccg tgtgcgagac 13740
accgcggccg gccgccggcg ttgtggatac ctcgcggaaa acttggccct cactgacaga 13800
tgaggggcgg acgttgacac ttgaggggcc gactcacccg gcgcggcgtt gacagatgag 13860
gggcaggctc gatttcggcc ggcgacgtgg agctggccag cctcgcaaat cggcgaaaac 13920
gcctgatttt acgcgagttt cccacagatg atgtggacaa gcctggggat aagtgccctg 13980
cggtattgac acttgagggg cgcgactact gacagatgag gggcgcgatc cttgacactt 14040
gaggggcaga gtgctgacag atgaggggcg cacctattga catttgaggg gctgtccaca 14100
ggcagaaaat ccagcatttg caagggtttc cgcccgtttt tcggccaccg ctaacctgtc 14160
ttttaacctg cttttaaacc aatatttata aaccttgttt ttaaccaggg ctgcgccctg 14220
gcgcgtgacc gcgcacgccg aaggggggtg cccccccttc tcgaaccctc ccggcccgct 14280
aacgcgggcc tcccatcccc ccaggggctg cgcccctcgg ccgcgaacgg cctcacccca 14340
aaaatggcag ccaagctcct aacattttat tagagagcag gctagttgct tagatacatg 14400
atcttcaggc cgttatctgt cagggcaagc gaaaattggc catttatgac gaccaatgcc 14460
ccgcagaagc tcccatcttt gccgccatag acgccgcgcc ccccttttgg ggtgtagaac 14520
atccttttgc cagatgtgga aaagaagttc gttgtcccat tgttggcaat gacgtagtag 14580
ccggcgaaag tgcgagaccc atttgcgcta tatataagcc tacgatttcc gttgcgacta 14640
ttgtcgtaat tggatgaact attatcgtag ttgctctcag agttgtcgta atttgatgga 14700
ctattgtcgt aattgcttat ggagttgtcg tagttgcttg gagaaatgtc gtagttggat 14760
ggggagtagt catagggaag acgagcttca tccactaaaa caattggcag gtcagcaagt 14820
gcctgccccg atgccatcgc aagtacgagg cttagaacca ccttcaacag atcgcgcata 14880
gtcttcccca gctctctaac gcttgagtta agccgcgccg cgaagcggcg tcggcttgaa 14940
cgaattgtta gacattattt gccgactacc ttggtgatct cgcctttcac gtagtgaaca 15000
aattcttcca actgatctgc gcgcgaggcc aagcgatctt cttgtccaag ataagcctgc 15060
ctagcttcaa gtatgacggg ctgatactgg gccggcaggc gctccattgc ccagtcggca 15120
gcgacatcct tcggcgcgat tttgccggtt actgcgctgt accaaatgcg ggacaacgta 15180
agcactacat ttcgctcatc gccagcccag tcgggcggcg agttccatag cgttaaggtt 15240
tcatttagcg cctcaaatag atcctgttca ggaaccggat caaagagttc ctccgccgct 15300
ggacctacca aggcaacgct atgttctctt gcttttgtca gcaagatagc cagatcaatg 15360
tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat tgcgctgcca ttctccaaat 15420
tgcagttcgc gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg 15480
acttctacag cgcggagaat ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg 15540
ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac cagcaaatca 15600
atatcactgt gtggcttcag gccgccatcc actgcggagc cgtacaaatg tacggccagc 15660
aacgtcggtt cgagatggcg ctcgatgacg ccaactacct ctgatagttg agtcgatact 15720
tcggcgatca ccgcttccct catgatgttt aactcctgaa ttaagccgcg ccgcgaagcg 15780
gtgtcggctt gaatgaattg ttaggcgtca tcctgtgctc ccgagaacca gtaccagtac 15840
atcgctgttt cgttcgagac ttgaggtcta gttttatacg tgaacaggtc aatgccgccg 15900
agagtaaagc cacattttgc gtacaaattg caggcaggta cattgttcgt ttgtgtctct 15960
aatcgtatgc caaggagctg tctgcttagt gcccactttt tcgcaaattc gatgagactg 16020
tgcgcgactc ctttgcctcg gtgcgtgtgc gacacaacaa tgtgttcgat agaggctaga 16080
tcgttccatg ttgagttgag ttcaatcttc ccgacaagct cttggtcgat gaatgcgcca 16140
tagcaagcag agtcttcatc agagtcatca tccgagatgt aatccttccg gtaggggctc 16200
acacttctgg tagatagttc aaagccttgg tcggataggt gcacatcgaa cacttcacga 16260
acaatgaaat ggttctcagc atccaatgtt tccgccacct gctcagggat caccgaaatc 16320
ttcatatgac gcctaacgcc tggcacagcg gatcgcaaac ctggcgcggc ttttggcaca 16380
aaaggcgtga caggtttgcg aatccgttgc tgccacttgt ttaatagact ggatggaggc 16440
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 16500
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 16560
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 16620
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 16680
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 16740
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 16800
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgatatcctt tttttctgcg 16860
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 16920
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 16980
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 17040
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 17100
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 17160
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 17220
acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 17280
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 17340
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 17400
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 17460
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 17520
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 17580
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 17640
tctgtgcggt atttcacacc gcatatgaag atcggcggca atagcttctt agcgccatcc 17700
cggctgagaa agcccagtaa ggaaacaact gtaggttcga gtcgcgagat cccccggaac 17760
caaaggaagt aggttaaacc cgctccgatc aggccgagcc acgccaggcc gagaacattg 17820
gttcctgtag gcatcgggat tggcggatca aacactaaag ctactggaac gagcagaagt 17880
cctccggccg ccagttgcca ggcggtaaag gtgagcagag gcacgggagg ttgccacttg 17940
cgggtcagca cggttccgaa cgccatggaa accgcccccg ccaggcccgc tgcgacgccg 18000
acaggatcta gcgctgcgtt tggtgtcaac accaacagcg ccacgcccgc agttccgcaa 18060
atagccccca ggaccgccat caatcgtatc gggctaccta gcagagcggc agagatgaac 18120
acgaccatca gcggctgcac agcgcctacc gtcgccgcga ccccgcccgg caggcggtag 18180
accgaaataa acaacaagct ccagaatagc gaaatattaa gtgcgccgag gatgaagatg 18240
cgcatccacc agattcccgt tggaatctgt cggacgatca tcacgagcaa taaacccgcc 18300
ggcaacgccc gcagcagcat accggcgacc cctcggcctc gctgttcggg ctccacgaaa 18360
acgccggaca gatgcgcctt gtgagcgtcc ttggggccgt cctcctgttt gaagaccgac 18420
agcccaatga tctcgccgtc gatgtaggcg ccgaatgcca cggcatctcg caaccgttca 18480
gcgaacgcct ccatgggctt tttctcctcg tgctcgtaaa cggacccgaa catctctgga 18540
gctttcttca gggccgacaa tcggatctcg cggaaatcct gcacgtcggc cgctccaagc 18600
cgtcgaatct gagccttaat cacaattgtc aattttaatc ctctgtttat cggcagttcg 18660
tagagcgcgc cgtgcgtccc gagcgatact gagcgaagca agtgcgtcga gcagtgcccg 18720
cttgttcctg aaatgccagt aaagcgctgg ctgctgaacc cccagccgga actgacccca 18780
caaggcccta gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt tccaccaggc 18840
cgctgcctcg caactcttcg caggcttcgc cgacctgctc gcgccacttc ttcacgcggg 18900
tggaatccga tccgcacatg aggcggaagg tttccagctt gagcgggtac ggctcccggt 18960
gcgagctgaa atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg tacttctccc 19020
atatgaattt cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg tcgatcagga 19080
cctggcaacg ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc agcagcgaca 19140
ccgattccag gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc tgtaggcgcg 19200
acaggcattc ctcggccttc gtgtaatacc ggccattgat cgaccagccc aggtcctggc 19260
aaagctcgta gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc gcgtactcca 19320
acacctgctg ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg gtgtaggtga 19380
tcttcacgtc cttgttgacg tggaaaatga ccttgttttg cagcgcctcg cgcgggattt 19440
tcttgttgcg cgtggtgaac agggcagagc gggccgtgtc gtttggcatc gctcgcatcg 19500
tgtccggcca cggcgcaata tcgaacaagg aaagctgcat ttccttgatc tgctgcttcg 19560
tgtgtttcag caacgcggcc tgcttggcct cgctgacctg ttttgccagg tcctcgccgg 19620
cggtttttcg cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc gacttcgcca 19680
aacctgccgc ctcctgttcg agacgacgcg aacgctccac ggcggccgat ggcgcgggca 19740
gggcaggggg agccagttgc acgctgtcgc gctcgatctt ggccgtagct tgctggacca 19800
tcgagccgac ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg cttgcgatgg 19860
tttcggcatc ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat gccttccggt 19920
caaacgtccg attcattcac cctccttgcg ggattgcccc gactcacgcc ggggcaatgt 19980
gcccttattc ctgatttgac ccgcctggtg ccttggtgtc cagataatcc accttatcgg 20040
caatgaagtc ggtcccgtag accgtctggc cgtccttctc gtacttggta ttccgaatct 20100
tgccctgcac gaataccagc tccgcgaagt cgctcttctt gatggagcgc atggggacgt 20160
gcttggcaat cacgcgcacc ccccggccgt tttagcggct aaaaaagtca tggctctgcc 20220
ctcgggcgga ccacgcccat catgaccttg ccaagctcgt cctgcttctc ttcgatcttc 20280
gccagcaggg cgaggatcgt ggcatcaccg aaccgcgccg tgcgcgggtc gtcggtgagc 20340
cagagtttca gcaggccgcc caggcggccc aggtcgccat tgatgcgggc cagctcgcgg 20400
acgtgctcat agtccacgac gcccgtgatt ttgtagccct ggccgacggc cagcaggtag 20460
gccgacaggc tcatgccggc cgccgccgcc ttttcctcaa tcgctcttcg ttcgtctgga 20520
aggcagtaca ccttgatagg tgggctgccc ttcctggttg ggtaatgact ccaacttatt 20580
gatagtgttt tatgttcaga taatgcccga tgactttgtc atgcagctcc accgattttg 20640
agaacgacag cgacttccgt cccagccgtg ccaggtgctg cctcagattc aggttatgcc 20700
gctcaattcg ctgcgtatat cgcttgctga ttacgtgcag ctttcccttc aggcgggatt 20760
catacagcgg ccagccatcc gtcatccata tcaccacgtc aaagggtgac agcaggctca 20820
taagacgccc cagcgtcgcc atagtgcgtt caccgaatac gtgcgcaaca accgtcttcc 20880
ggagactgtc atacgcgtaa aacagccagc gctggcgcga tttagccccg acatagcccc 20940
actgttcgtc catttccgcg cagacgatga cgtcactgcc cggctgtatg cgcgaggtta 21000
ccgactgcgg cctgagtttt ttaagtgacg taaaatcgtg ttgaggccaa cgcccataat 21060
gcgggctgtt gcccggcatc caacgccatt catggccata tcaatgattt tctggtgcgt 21120
accgggttga gaagcggtgt aagtgaactg cagttgccat gttttacggc agtgagagca 21180
gagatagcgc tgatgtccgg cggtgctttt gccgttacgc accaccccgt cagtagctga 21240
acaggaggga cagctgatag aaacagaagc cactggagca cctcaaaaac accatcatac 21300
actaaatcag taagttggca gcatcacccc tggttggctt ggtttcatca gccatccgct 21360
tgccctcatc tgttacgccg gcggtagccg gccagcctcg cagagcagga ttcccgttga 21420
gcaccgccag gtgcgaataa gggacagtga agaaggaaca cccgctcgcg ggtgggccta 21480
cttcacctat cctgcccggc tgacgccgtt ggatacacca aggaaagtct acacgaaccc 21540
tttggcaaaa tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa atcgctataa 21600
tgaccccgaa gcagggttat gcagcggaaa agatcc 21636
<210> 153
<211> 21890
<212> DNA
<213> 人工序列
<220>
<223> 转化构建体
<400> 153
gtcgacgatc gtcaacgttc acttctaaag aaatagcgcc actcagcttc ctcagcggct 60
ttatccagcg atttcctatt atgtcggcat agttctcaag atcgacagcc tgtcacggtt 120
aagcgagaaa tgaataagaa ggctgataat tcggatctct gcgagggaga tgatatttga 180
tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc 240
cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca 300
aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg 360
tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca 420
ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt 480
ttttaatgta ctgaattcca atccccaaaa atctgagctt aacagcacag ttgctcctct 540
cagagcagaa tcgggtattc aacaccctca tatcaactac tacgttgtgt ataacggtcc 600
acatgccggt atatacgatg actggggttg tacaaaggcg gcaacaaacg gcgttcccgg 660
agttgcacac aagaaatttg ccactattac agaggcaaga gcagcagctg acgcgtacac 720
aacaagtcag caaacagaca ggttgaactt catccccaaa ggagaagctc aactcaagcc 780
caagagcttt gctaaggccc taacaagccc accaaagcaa aaagcccact ggctcacgct 840
aggaaccaaa aggcccagca gtgatccagc cccaaaagag atctcctttg ccccggagat 900
tacaatggac gatttcctct atctttacga tctaggaagg aagttcgaag gtgaaggtga 960
cgacactatg ttcaccactg ataatgagaa ggttagcctc ttcaatttca gaaagaatgc 1020
tgacccacag atggttagag aggcctacgc agcaggtctc atcaagacga tctacccgag 1080
taacaatctc caggagatca aataccttcc caagaaggtt aaagatgcag tcaaaagatt 1140
caggactaat tgcatcaaga acacagagaa agacatattt ctcaagatca gaagtactat 1200
tccagtatgg acgattcaag gcttgcttca taaaccaagg caagtaatag agattggagt 1260
ctctaaaaag gtagttccta ctgaatctaa ggccatgcat ggagtctaag attcaaatcg 1320
aggatctaac agaactcgcc gtgaagactg gcgaacagtt catacagagt cttttacgac 1380
tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca cgacactctg gtctactcca 1440
aaaatgtcaa agatacagtc tcagaagacc aaagggctat tgagactttt caacaaagga 1500
taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc atcgaaagga 1560
cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggctatca 1620
ttcaagatct ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg 1680
tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gacatctcca 1740
ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc tctatataag 1800
gaagttcatt tcatttggag aggacacgct cgagtataag agctctattt ttacaacaat 1860
taccaacaac aacaaacaac aaacaacatt acaattacta tttacaatta ccatgagccc 1920
agaacgacgc ccggccgaca tccgccgtgc caccgaggcg gacatgccgg cggtctgcac 1980
catcgtcaac cactacatcg agacaagcac ggtcaacttc cgtaccgagc cgcaggaacc 2040
gcaggagtgg acggacgacc tcgtccgtct gcgggagcgc tatccctggc tcgtcgccga 2100
ggtggacggc gaggtcgccg gcatcgccta cgcgggcccc tggaaggcac gcaacgccta 2160
cgactggacg gccgagtcaa ccgtgtacgt ctccccccgc caccagcgga cgggactggg 2220
ctccacgctc tacacccacc tgctgaagtc cctggaggca cagggcttca agagcgtggt 2280
cgctgtcatc gggctgccca acgacccgag cgtgcgcatg cacgaggcgc tcggatatgc 2340
cccccgcggc atgctgcggg cggccggctt caagcacggg aactggcatg acgtgggttt 2400
ctggcagctg gacttcagcc tgccggtacc gccccgtccg gtcctgcccg tcaccgagat 2460
ttgatgaccc gggggatcct agagtcctgc tttaatgaga tatgcgagac gcctatgatc 2520
gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct 2580
cagatcctta ccgccggttt cggttcattc taatgaatat atcacccgtt actatcgtat 2640
ttttatgaat aatattctcc gttcaattta ctgattgtac cctactactt atatgtacaa 2700
tattaaaatg aaaacaatat attgtgctga ataggtttat agcgacatct atgatagagc 2760
gccacaataa caaacaattg cgttttatta ttacaaatcc aattttaaaa aaagcggcag 2820
aaccggtcaa acctaaaaga ctgattacat aaatcttatt caaatttcaa aagtgcccca 2880
ggggctagta tctacgacac accgagcggc gaactaataa cgctcactga agggaactcc 2940
ggttccccgc cggcgcgcat gggtgagatt ccttgaagtt gagtattggc cgtccgctct 3000
accgaaagtt acgggcacca ttcaacccgg tccagcacgg cggccgggta accgacttgc 3060
tgccccgaga attatgcagc atttttttgg tgtatgtggg ccccaaatga agtgcaggtc 3120
aaaccttgac agtgacgaca aatcgttggg cgggtccagg gcgaattttg cgacaacatg 3180
tcgaggctca gcaggacctg caggcatgca agctctagag cggccgcttt aagagtgttg 3240
aggatgtgct tacttaaatt tcaaaagggc tatttaggga taatatttgg tgaagcatgg 3300
ctggtggact gaccataggt acccactgaa taaacatatc agcccaaagg cagagggtat 3360
ccctccctgt aatacgtact agtattcgag gagcgtgcta aagtagtatc ctctcttgat 3420
gctcctcgtc tccctccact gagtactccc ttcctctcct cattctcatc tcagtcctct 3480
cctcctccct cgaataagat agatccgccc ttgttgctga tctcatctcg cctcctattg 3540
gcctcgcccc tgccaactga ctgctctatt ggtgaccgtg gactttttgg gtatatatat 3600
ttcctgtcga tttgtttcca cttaaagtag caatgcttgg atctaaaact tattacttct 3660
cctgcttcag attctgatcc atttgtgttg attgaactca gggtgggatc gtggggctct 3720
gtatctatct aggatggata ttccaagcaa tttactctct tgagtgctgt tggtcttcct 3780
ctgctcctcg tctccgcttc ctctcagtct cctccattgc gctgcctgga acagatcgat 3840
ccgccttgtt gctgatctca tccatcctcc gtggagattt cgggtggcag tttccagtga 3900
ctattcagag caccatacta gtggcctggt aaaaatttcc ctcccatctt tcttgctatt 3960
ctcttcttat taatctgctg atttgcttga tggtaagttc ttacttattc ttccagatcg 4020
agtgaagcga catccgccgc cgtcttgtcc cctacgacgc cgctgtgttc tgctctgtcc 4080
tcctctgccg gtgaagcatc aacggtgacg gccatccggt gttgacgccg cggctgtgtt 4140
ctcctctgcc gtgaagtatc aaaggtgggt ttggatggat atcacagttt ggattggcaa 4200
ctctgttcta tagctcgttg aaattaaagc ttcacacagt tttgattgga ttacagctcc 4260
ccatattcat ctattttgca ccatgcatcg aatccactga cctccacacg ctcacggatc 4320
tggcctctgg tgcccacacg ctcacggatc cgacagagtg ctcccttcct atcatctcag 4380
tcctcatctc ctggtcgatc cggcttcttg tttggtgagc tcgtccaacc tccgagctcg 4440
cccctgacac ctgtggagtg gaggtatatt tctttccagc tgaagtacgc ttggatctaa 4500
aaacttacgt ctcctgcttc actgcttgct tgggagtctg atcgatttgt gttggttgaa 4560
tcatttgggg tggcagtgga gcagctagca agaatcatgg agtttccaat cattattcag 4620
agcaccacgc atggaatcca ctgacctggt aactttgtct catctttctt gctactgaaa 4680
tcttccacat ccccaccttc cttgttcatc tgctgatttc ccagattata cttccagatt 4740
gagtgaagcg ccgtcttgtc ccctcacgga tctggcctct ggtgcccagc cgccgctttc 4800
gcttcgttgt ccgacgaggc caccgccgct gtgttctgct ctgccgtgaa gcacaaaaag 4860
gtggggtctt cctctgctct ttctccactg acggcgatcc gaccttgaca cccgtggccg 4920
tggcagtggg gtcttcctct gctctgtttt tttaccagtt attggatcta aaaagggatc 4980
taaaagttat tacttctcct gcttgagatt ctgaccgatt tgtgttgatt gaatccaggg 5040
tggggtcgtg gggctctgta tctatctagt acggagtacc actgtattcc aagcaattta 5100
ctctcttgac tgctgttgga cttcctctgt ccccgcttcc attgtgctgc cttgaacaga 5160
tccatccacc ttgttgctaa tctcatccaa cctccgggta tgtttcttgt cgatttgttt 5220
ccagtaaagt agcaatgctt gggtctaaaa agtcattgct tctcctgctt gacgagtctc 5280
attgatttgc ccagtcatta ttcagagcac catgcatcca aggcgctgac ccggctataa 5340
gtttttatta cagggccgcc gtgttgtccc catcgagcaa agcgtttaat ccactgaccc 5400
gcttcgttgt ccgcacagcg tgaccatccg acgaagacgc cgccgttgtg ttctgctctc 5460
ctctgaagca tcaaaaggtg ggcttggtcc agatccccac attcatcgat ttggcttgcc 5520
aactcagttc caccagagct tggattgtgt tacagatccc taaattcatc gatttgcagg 5580
tttcagctga ttgatcaaga gagctctcaa tgatggcgga agttctggtg agcgcatcca 5640
caggggcaat gggttccctc ctgcggaagt tgggagctat gttgacggat gaatacaagc 5700
tgctcaagaa cgtccgtggg gacatcaagt tcctcaagga cgagctggag gtcatgtgcg 5760
cgttcctcct caagatgtca gatgtggagg agcctgacga accaacaaag ctgcgtgtga 5820
cggcggtgcg ggagatgtcc tataagatag aggacaacat cgacaagttc atggtcctcg 5880
tggaacagga acacgggtcc tcctgttccg aggcggctca tggcgtcgcc aagctcatgg 5940
acaagtgcaa gaacttgctg ccggacatca aggcccgccg caggatcgcc aaggaggtca 6000
aagacatcaa gaaagaaatt aaggatgtca gcgacagatt ttcaaggtac cagatcttgt 6060
tctccttaca gtactattag tacctaccat cagatttgtg accgatttgt tgccactcaa 6120
ggtacaagat tgacgactcc tcctcctcta tgccggcaaa agacaaggtc gaccctcgac 6180
ttcgtgcagt ctacaaagac gccgcagagc tcgtcggaat tgatggacca aaggatgaac 6240
ttgtcaagtg gctgaatgag aaagaaggcc aatcactgaa atctgtctct attgttggat 6300
atggagggtt gggcaagacc acgctcgcca accagatccg ggtcaacctt ggagcgacct 6360
tcgactgcgg ggcttttgtc tcgatttcac gcaagcctga catgaaggcg atattaagat 6420
ctatattatc acaaatcacc aagaaagacg atgcttgctc tagattagat gatatacaac 6480
tcatcatcga caagattcga gaattcctcc aagacacaag gtatgtatgt gagatctgct 6540
acgaagtaac attgcttaat tagctcatgc ataatcggtt gcctagaatt ttgctaatga 6600
agattgcttt gtgtgattta ctttcgtcct caacttgtat gactgcgctt aaactttcaa 6660
gtcattgttt cttgcaactt atgtcgatct gttcactaat taagatatag ctaaaatgtg 6720
tttgccgatt tctgtcgcca cacattttct aatttgattg ccacaagttt gttaaacttg 6780
cggctatgtt ctgtttatta aataattgtg acatggcgtc agtcaaaaca ataaagtaca 6840
tgcaattccc tctcttttgt acaagataaa tatccgcacg cactacatga ctgcaaatat 6900
atctatcttc taaggaccca aagcacttgc atgtgcagac cattgttacc ggatatgcag 6960
tacattaggt ttgtgattag ccactttaag aacatagggt ttctagaggc tataaaccta 7020
cggttatcag tattataatc ttgaatggat atcaaacatc cgttgaagaa tctatgccca 7080
taacacaaaa agagaaaagg gaagaattaa tcatagtcag atcggaccag cctgactagc 7140
gtaacataat gacaacagta ggttgttgcg tctttcctgg tttcgtagca atttgcatga 7200
cgaattgagg cgcagagcca ccactcaata tgtggtccta ccaatcatga ggaaataact 7260
tgttaccaaa atgacaaaag aaaataggta gtccccatca ttgtttcaat atctatcctc 7320
ttgcgaacat gctattggca gtggcaaaac attgtacctt gccactggca acagctgcct 7380
ttactcggcc tgagcattca tatttttttc ctcttggttg ttaaatcttg ctatttttcg 7440
cttgccttct atggcaatct ttttttttct tatattgcca ccaaattgta tctaagagca 7500
actccaacac gccgacgcaa tttttgtcca tttagatcgg ccaagcggac ggggtgtctg 7560
gttttgcctt tgggtggtgc gcctacgcat tagtccgcgt gggcatttaa aaaacttaaa 7620
aagcttaaaa taattaaata taaataaaaa tgcatattaa agcccgtcaa agtccacata 7680
attaaacata atacattaat atattaataa aaaacagccg ccccagcccg cagccctagg 7740
cgtcttcgtc ggaggagtgg gtgaggtcga agaatgccgg cagctcagcc cacgggaagg 7800
cctgctggtg ggcagccagc accgcctgct ccgctgtctg cggcggcggc ggccctacct 7860
ggcaccgcgc acgcctcctc ctcgcgctcc tgccgctcct cctcctcatc gcacgcccaa 7920
cgaagttttg gcgctcctcc ccctcgggtt cccgctgcat gcggacctgg ccgtcgtacc 7980
gctcctcctc cagcgtgcgg tccttccacg ccacgaggta ggccgcctcc tcctccatca 8040
cgcacagcca aatgggcggc ttgctcatcc gcttgcagac gatgccctgc cattggtaaa 8100
cgccggtggg cgcgggtggt gctagcgctg gtgcaggcgg tgacgatgtg gctggtggtc 8160
ccggcaggta gacactatag ctggcggccg atagctgcag cgtgtcatcg aggccagacc 8220
actaggagtc ctcgcactaa gactcctcgg tgacgtgatg ggccccctcc tccaatgcct 8280
tgaggaacgc cgcctcctcc tcaacctact atttgaccac cggtggcggc ggtggagggc 8340
aggcttcgag ctggacggcg tcgcggaggc gcacatggtg ctcgatggtg aaccagactt 8400
ctcagttagg ggagtctggc ctcgatgggt ggagtcgcga cgctgctctg gcgtcaggag 8460
agcacgccgg gcccgaatct cgttggcgcg cgcacgctcg gactgtggca ccgccggcac 8520
tggaatgtgg tgggggtcga ggtgccagcc gtgggggaag ttcacgtccg gccacggcag 8580
tgagacgcgg tactcccggt ggtactaact acgcgtggtg caccgggatg tactggtggt 8640
cccgtggcgc tgccgccctc ccatcccgca gcgccgacac caatgcgaac cccttcgctg 8700
ccggcctcac cggcccttgc aacgagccgg cctcgtggtc gtgcttgcct tgcccttacc 8760
tttgccaaag aaacccatga cggctagggt tttctgtcac cggcgaggga gcagatgtgg 8820
actagagtgg gaggagaagt ggatgaggcc cgccccgccg cacccttgca attaaaaagg 8880
gcgtgatccg gacgtttcta gtcactgcca tgcgggcccg aggtaggtgg gcgccattga 8940
ctacgtctgt ggtggtagtt ggctggcaga cacgccagcc cgaggcgcac accgagcgga 9000
cgcgcaacac gtccgtctcg tgttcgtgtg aacgcaaatc aggcacaaat ttgggctgga 9060
aatgcgtcta catggatgcc gaacggacgt gatttgggtt tgtgtccgca cgttgggccg 9120
gcgtttttgt gcctttcgac cctaatggac acacacggac caaatccgtt ggcgtgttag 9180
agttgcttgt acctagtact actatgtgtg gagttgttat ctttgagttt ctcccccatc 9240
tgtagctgtg tattattgtg agatgctctc aggatttcac ttattttaga taatgacaac 9300
tttcttgatg ctagacaatt acatcaaggt gtattatatc taggatgcac acagatgtga 9360
gattgtttca ggattttagg ttacttggta ggtgttacta taaatatcta atgagctcta 9420
atgtgtatgt tcatgaaact gtgttgcagg tactttatca taattgatga tatatgggag 9480
ttaggaacat gggaaacttt gaaatgtgca tttgtcaaga atacattggg cagcagaata 9540
attatcacaa cacgtatagt tgatgttgcc aaatcttgtt ctccttctag tgaagatctt 9600
gtctatgaga tgaaaccact cagtgaggct gactcgaaga aattgttttt caagaggata 9660
tttggctgtg aagaaagctg tcctgatagc ttgaaagaag ctgccaatga tattttgaaa 9720
aaatgccgtg gcctacctct ggccatcaat gccatttcta gcgtgttggt cacaacaagg 9780
gaaacaaaag aagagtggga tcgggtgcga cattcaatac gctcttcaaa ggtcaaaagt 9840
gatataatag aaaccatgaa ttacatattg tctcttagtt attttgatct tccccaccac 9900
ctaagaagct gcttattgta cctggctctg tttcctgaag accaattgat tggaaggaag 9960
cggttagtac gcagatggat ttctgaaggc tttatccatg gagaaagtgg acaagatctc 10020
atggaattag gagaggaata ttttcaccag cttgtgaaca gaagtctaat acagcccggc 10080
aacatagggt atgacggcaa ggcaatgtat tgccgagtcc atgacaccat ccttgatttc 10140
ctaattgaca aatcctctga agagaacatg tgtactgtcc taaagaaaca atgcaagcct 10200
aatggcatag ttcgtcggct ctctctaatg gggaatgagg atgaagaaat cgtggagcaa 10260
ttggatctgt cacacgctcg gtcaatcact gcttttgggg atatcaagct attgccttcg 10320
cttgggaggt caaaatgctt gcgcgtgcta gacttgcaag actgcgatca gttggaaaat 10380
catcatatca aggatattga aagactctat cagctaagat acttggatat ctcttctaca 10440
ggaatcacgg agcttcccag gcaaattggg gagttgctgt atctagagac gctagttgcc 10500
tatggattac gtgagcttcc tgaaagcaca agtcggctac aacgactggc acgtttgttt 10560
gtttactctg gttgtaagct tccaggtggg ttgggaaact tgataaactt gcaagagctt 10620
gactgcgttg atgccttgca tttgaagcat gtggaagagc tcggcaaact aacaaatctg 10680
agaaagctga gtattaaatt ggacactggt gggattgaag gcaacaaatt agaagagtcc 10740
aaggaaaagc tggtgtcctc tctctgtaaa ctggacgaat gcggcctcct ttccctgagt 10800
attgattatt atctgagaga aaaggatgga gaagagccgt tcctccctgc tttgggttgt 10860
atccaggagg tttttgtata tggacaagat atcagccgga ttagcagatg gctcgcgtca 10920
cttcccaacc tacacaggtt gcttttggac gatccgaaga tagagcagca ggatattgag 10980
atgattggat tgatacccaa tctgatcgat ttgacactgc cgcctctata caaaacagac 11040
gacgctgggc ggctcatcat caggcgtgaa ggatttcaac agttgcagaa gtttgaggct 11100
tacaatactc gtatgggggt tttaatgttt gaaccggggg ctatgccgag gctcaaggag 11160
cttaaactcc ataacttcat cgaaaagccc aaatctgctg cggtcgattt cgactttggc 11220
atccaacgcc tctccagcct tgctcgcctc actgtcagct tatcctgcgg tggctggacg 11280
gtggcagaag tagaggctgc ggaggatgct ttcaagagca tggccgaggc gaaccccaac 11340
cgtccaatac tggagatgac aagatataat acgcagcata tgttacaaga cgagcaaata 11400
ggtatgacag gctcaggtac tattaactag tttgtacttt tgtcttggtg agcagtagat 11460
caaaagatgt gcatatgctt caatcttttt tccggtaatc tatttcagct acaacgccgg 11520
ccgtcaagtc ctaggcttct gacgttgctt caggtgtgct ctcaatctat acatatttac 11580
tcatcatatt attctccacc tttttttcca tctctagagc tcagcttgtc attgcattgt 11640
tcaattgtgc ttctctagca gctacggaaa ttctgaacag gcaaaggtaa ttttacagaa 11700
gtttctttgg cataagtatc tgagaaaaat gcacttggca caatttacct tttcgcagta 11760
gaaaagagtc agtggatcat accttcttaa ttgttcgatt gctcgtaata cttgggggca 11820
ctctatgatt gctgtgtgct tttagttttg atatagttcc tgttagcatg gacagactgt 11880
gtgattggtt catgaatttc agggtggttc taatgaggaa atgatgcttg cttcagaaat 11940
agtttccatt tgtacctaag ctgtgtagtt ggtaaagttg cattttggat gttctgttgg 12000
tatagcttac agaagaaaga cttgcgttgt gcaggtacca ggggcgcagg ttactgctag 12060
tggcgtctga gatgtttcat aaacgttggg tgggcaccgg tcatgcaaaa aattgaagga 12120
taaaaaggtt ccgagaggtt gtgtgatgtg ctccttttaa atggcagtcg cttgcataaa 12180
gagatgttgc ttggtttttt agtaaagcgt tgtagtaagt tgctctgttt cggctgagag 12240
ccatcgttgt tgctgtctgt tacatcgaat ggctggctca tgtcatgttg atgttttttt 12300
tatctgaacc ggacaaacct gagggtgtag tatcatgttt gtaatgactg aaattctggg 12360
ctcacccgag ctccttaatt cgtaaacaac tgcgactcat tgttggattc cttacttcag 12420
aaaatattta cttgctctgg ctcatacagc tctgcatgtt ttgtagtggt ttgtcgctgt 12480
ttaggtgctg cttcagactc ctggtacctt tggccgctgc tgctactgca tgagctttgt 12540
atggcattgt tagtatagtt tggtgtcatt gggttttcgc cccatggtga gtgtccattg 12600
cgcgcactcg cagcggtttc ctggcgataa gttgaacttg atctttctct ttcccacctc 12660
ctaaacgtct attcagactt gtgtatattt gcattacggg gattacccga gttcaaaaat 12720
aaatattatt tactagttct gccaatgtgt ttccgttaca tacaaataaa agggtgcttc 12780
cagctacaag tatatttttg agaagggcca ttgcaaaacg aaatgaagcc caaattctac 12840
caacatgttt ctgtcacata tacattaaaa aaacggtgta aatgtcccgt caaaaaaggg 12900
tgtatgtagg aaaaggagtt cgacgccgcc agctccctcc taacgctcag attgtcttcg 12960
cggccgccac cgcggctgag tggctccttc aagcttgcgg atccgggcgg aaataggtaa 13020
agaagttgcg gataaggtaa ttgccattgc agattatttg gattgagagt gaatatgaga 13080
ctctaattgg ataccgaggg gaatttatgg aacgtcagtg gagcattttt gacaagaaat 13140
atttgctagc tgatagtgac cttaggcgac ttttgaacgc gcaataatgg tttctgacgt 13200
atgtgcttag ctcattaaac tccagaaacc cgcggctgag tggctccttc aacgttgcgg 13260
ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca tcggcggggg tcataacgtg 13320
actcccttaa ttctccgctc atgatcagat tgtcgtttcc cgccttcagt ttaaactatc 13380
agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa 13440
tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca 13500
accacagggt tcccctcggg agtgcttggc attccgtgcg ataatgactt ctgttcaacc 13560
acccaaacgt cggaaagcct gacgacggag cagcattcca aaaagatccc ttggctcgtc 13620
tgggtcggct agaaggtcga gtgggctgct gtggcttgat ccctcaacgc ggtcgcggac 13680
gtagcgcagc gccgaaaaat cctcgatcgc aaatccgacg ctgtcgaaaa gcgtgatctg 13740
cttgtcgctc tttcggccga cgtcctggcc agtcatcacg cgccaaagtt ccgtcacagg 13800
atgatctggc gcgagttgct ggatctcgcc ttcaatccgg gtctgtggcg ggaactccac 13860
gaaaatatcc gaacgcagca agatcgtcga cccctttccg acgctcaccg ggctggttgc 13920
cctcgccgct gggctggcgg ccgtctatgg ccctgcaaac gcgccagaaa cgccgtcgaa 13980
gccgtgtgcg agacaccgcg gccggccgcc ggcgttgtgg atacctcgcg gaaaacttgg 14040
ccctcactga cagatgaggg gcggacgttg acacttgagg ggccgactca cccggcgcgg 14100
cgttgacaga tgaggggcag gctcgatttc ggccggcgac gtggagctgg ccagcctcgc 14160
aaatcggcga aaacgcctga ttttacgcga gtttcccaca gatgatgtgg acaagcctgg 14220
ggataagtgc cctgcggtat tgacacttga ggggcgcgac tactgacaga tgaggggcgc 14280
gatccttgac acttgagggg cagagtgctg acagatgagg ggcgcaccta ttgacatttg 14340
aggggctgtc cacaggcaga aaatccagca tttgcaaggg tttccgcccg tttttcggcc 14400
accgctaacc tgtcttttaa cctgctttta aaccaatatt tataaacctt gtttttaacc 14460
agggctgcgc cctggcgcgt gaccgcgcac gccgaagggg ggtgcccccc cttctcgaac 14520
cctcccggcc cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc tcggccgcga 14580
acggcctcac cccaaaaatg gcagccaagc tcctaacatt ttattagaga gcaggctagt 14640
tgcttagata catgatcttc aggccgttat ctgtcagggc aagcgaaaat tggccattta 14700
tgacgaccaa tgccccgcag aagctcccat ctttgccgcc atagacgccg cgcccccctt 14760
ttggggtgta gaacatcctt ttgccagatg tggaaaagaa gttcgttgtc ccattgttgg 14820
caatgacgta gtagccggcg aaagtgcgag acccatttgc gctatatata agcctacgat 14880
ttccgttgcg actattgtcg taattggatg aactattatc gtagttgctc tcagagttgt 14940
cgtaatttga tggactattg tcgtaattgc ttatggagtt gtcgtagttg cttggagaaa 15000
tgtcgtagtt ggatggggag tagtcatagg gaagacgagc ttcatccact aaaacaattg 15060
gcaggtcagc aagtgcctgc cccgatgcca tcgcaagtac gaggcttaga accaccttca 15120
acagatcgcg catagtcttc cccagctctc taacgcttga gttaagccgc gccgcgaagc 15180
ggcgtcggct tgaacgaatt gttagacatt atttgccgac taccttggtg atctcgcctt 15240
tcacgtagtg aacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttgtc 15300
caagataagc ctgcctagct tcaagtatga cgggctgata ctgggccggc aggcgctcca 15360
ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa 15420
tgcgggacaa cgtaagcact acatttcgct catcgccagc ccagtcgggc ggcgagttcc 15480
atagcgttaa ggtttcattt agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga 15540
gttcctccgc cgctggacct accaaggcaa cgctatgttc tcttgctttt gtcagcaaga 15600
tagccagatc aatgtcgatc gtggctggct cgaagatacc tgcaagaatg tcattgcgct 15660
gccattctcc aaattgcagt tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt 15720
gcacaacaat ggtgacttct acagcgcgga gaatctcgct ctctccaggg gaagccgaag 15780
tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg 15840
taaccagcaa atcaatatca ctgtgtggct tcaggccgcc atccactgcg gagccgtaca 15900
aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat gacgccaact acctctgata 15960
gttgagtcga tacttcggcg atcaccgctt ccctcatgat gtttaactcc tgaattaagc 16020
cgcgccgcga agcggtgtcg gcttgaatga attgttaggc gtcatcctgt gctcccgaga 16080
accagtacca gtacatcgct gtttcgttcg agacttgagg tctagtttta tacgtgaaca 16140
ggtcaatgcc gccgagagta aagccacatt ttgcgtacaa attgcaggca ggtacattgt 16200
tcgtttgtgt ctctaatcgt atgccaagga gctgtctgct tagtgcccac tttttcgcaa 16260
attcgatgag actgtgcgcg actcctttgc ctcggtgcgt gtgcgacaca acaatgtgtt 16320
cgatagaggc tagatcgttc catgttgagt tgagttcaat cttcccgaca agctcttggt 16380
cgatgaatgc gccatagcaa gcagagtctt catcagagtc atcatccgag atgtaatcct 16440
tccggtaggg gctcacactt ctggtagata gttcaaagcc ttggtcggat aggtgcacat 16500
cgaacacttc acgaacaatg aaatggttct cagcatccaa tgtttccgcc acctgctcag 16560
ggatcaccga aatcttcata tgacgcctaa cgcctggcac agcggatcgc aaacctggcg 16620
cggcttttgg cacaaaaggc gtgacaggtt tgcgaatccg ttgctgccac ttgtttaata 16680
gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 16740
tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 16800
ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 16860
actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 16920
taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 16980
tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 17040
gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgatat 17100
cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 17160
gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 17220
gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac 17280
tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 17340
ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 17400
cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 17460
gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag 17520
gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 17580
gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 17640
cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 17700
tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 17760
cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc 17820
cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat 17880
tttctcctta cgcatctgtg cggtatttca caccgcatat gaagatcggc ggcaatagct 17940
tcttagcgcc atcccggctg agaaagccca gtaaggaaac aactgtaggt tcgagtcgcg 18000
agatcccccg gaaccaaagg aagtaggtta aacccgctcc gatcaggccg agccacgcca 18060
ggccgagaac attggttcct gtaggcatcg ggattggcgg atcaaacact aaagctactg 18120
gaacgagcag aagtcctccg gccgccagtt gccaggcggt aaaggtgagc agaggcacgg 18180
gaggttgcca cttgcgggtc agcacggttc cgaacgccat ggaaaccgcc cccgccaggc 18240
ccgctgcgac gccgacagga tctagcgctg cgtttggtgt caacaccaac agcgccacgc 18300
ccgcagttcc gcaaatagcc cccaggaccg ccatcaatcg tatcgggcta cctagcagag 18360
cggcagagat gaacacgacc atcagcggct gcacagcgcc taccgtcgcc gcgaccccgc 18420
ccggcaggcg gtagaccgaa ataaacaaca agctccagaa tagcgaaata ttaagtgcgc 18480
cgaggatgaa gatgcgcatc caccagattc ccgttggaat ctgtcggacg atcatcacga 18540
gcaataaacc cgccggcaac gcccgcagca gcataccggc gacccctcgg cctcgctgtt 18600
cgggctccac gaaaacgccg gacagatgcg ccttgtgagc gtccttgggg ccgtcctcct 18660
gtttgaagac cgacagccca atgatctcgc cgtcgatgta ggcgccgaat gccacggcat 18720
ctcgcaaccg ttcagcgaac gcctccatgg gctttttctc ctcgtgctcg taaacggacc 18780
cgaacatctc tggagctttc ttcagggccg acaatcggat ctcgcggaaa tcctgcacgt 18840
cggccgctcc aagccgtcga atctgagcct taatcacaat tgtcaatttt aatcctctgt 18900
ttatcggcag ttcgtagagc gcgccgtgcg tcccgagcga tactgagcga agcaagtgcg 18960
tcgagcagtg cccgcttgtt cctgaaatgc cagtaaagcg ctggctgctg aacccccagc 19020
cggaactgac cccacaaggc cctagcgttt gcaatgcacc aggtcatcat tgacccaggc 19080
gtgttccacc aggccgctgc ctcgcaactc ttcgcaggct tcgccgacct gctcgcgcca 19140
cttcttcacg cgggtggaat ccgatccgca catgaggcgg aaggtttcca gcttgagcgg 19200
gtacggctcc cggtgcgagc tgaaatagtc gaacatccgt cgggccgtcg gcgacagctt 19260
gcggtacttc tcccatatga atttcgtgta gtggtcgcca gcaaacagca cgacgatttc 19320
ctcgtcgatc aggacctggc aacgggacgt tttcttgcca cggtccagga cgcggaagcg 19380
gtgcagcagc gacaccgatt ccaggtgccc aacgcggtcg gacgtgaagc ccatcgccgt 19440
cgcctgtagg cgcgacaggc attcctcggc cttcgtgtaa taccggccat tgatcgacca 19500
gcccaggtcc tggcaaagct cgtagaacgt gaaggtgatc ggctcgccga taggggtgcg 19560
cttcgcgtac tccaacacct gctgccacac cagttcgtca tcgtcggccc gcagctcgac 19620
gccggtgtag gtgatcttca cgtccttgtt gacgtggaaa atgaccttgt tttgcagcgc 19680
ctcgcgcggg attttcttgt tgcgcgtggt gaacagggca gagcgggccg tgtcgtttgg 19740
catcgctcgc atcgtgtccg gccacggcgc aatatcgaac aaggaaagct gcatttcctt 19800
gatctgctgc ttcgtgtgtt tcagcaacgc ggcctgcttg gcctcgctga cctgttttgc 19860
caggtcctcg ccggcggttt ttcgcttctt ggtcgtcata gttcctcgcg tgtcgatggt 19920
catcgacttc gccaaacctg ccgcctcctg ttcgagacga cgcgaacgct ccacggcggc 19980
cgatggcgcg ggcagggcag ggggagccag ttgcacgctg tcgcgctcga tcttggccgt 20040
agcttgctgg accatcgagc cgacggactg gaaggtttcg cggggcgcac gcatgacggt 20100
gcggcttgcg atggtttcgg catcctcggc ggaaaacccc gcgtcgatca gttcttgcct 20160
gtatgccttc cggtcaaacg tccgattcat tcaccctcct tgcgggattg ccccgactca 20220
cgccggggca atgtgccctt attcctgatt tgacccgcct ggtgccttgg tgtccagata 20280
atccacctta tcggcaatga agtcggtccc gtagaccgtc tggccgtcct tctcgtactt 20340
ggtattccga atcttgccct gcacgaatac cagctccgcg aagtcgctct tcttgatgga 20400
gcgcatgggg acgtgcttgg caatcacgcg caccccccgg ccgttttagc ggctaaaaaa 20460
gtcatggctc tgccctcggg cggaccacgc ccatcatgac cttgccaagc tcgtcctgct 20520
tctcttcgat cttcgccagc agggcgagga tcgtggcatc accgaaccgc gccgtgcgcg 20580
ggtcgtcggt gagccagagt ttcagcaggc cgcccaggcg gcccaggtcg ccattgatgc 20640
gggccagctc gcggacgtgc tcatagtcca cgacgcccgt gattttgtag ccctggccga 20700
cggccagcag gtaggccgac aggctcatgc cggccgccgc cgccttttcc tcaatcgctc 20760
ttcgttcgtc tggaaggcag tacaccttga taggtgggct gcccttcctg gttgggtaat 20820
gactccaact tattgatagt gttttatgtt cagataatgc ccgatgactt tgtcatgcag 20880
ctccaccgat tttgagaacg acagcgactt ccgtcccagc cgtgccaggt gctgcctcag 20940
attcaggtta tgccgctcaa ttcgctgcgt atatcgcttg ctgattacgt gcagctttcc 21000
cttcaggcgg gattcataca gcggccagcc atccgtcatc catatcacca cgtcaaaggg 21060
tgacagcagg ctcataagac gccccagcgt cgccatagtg cgttcaccga atacgtgcgc 21120
aacaaccgtc ttccggagac tgtcatacgc gtaaaacagc cagcgctggc gcgatttagc 21180
cccgacatag ccccactgtt cgtccatttc cgcgcagacg atgacgtcac tgcccggctg 21240
tatgcgcgag gttaccgact gcggcctgag ttttttaagt gacgtaaaat cgtgttgagg 21300
ccaacgccca taatgcgggc tgttgcccgg catccaacgc cattcatggc catatcaatg 21360
attttctggt gcgtaccggg ttgagaagcg gtgtaagtga actgcagttg ccatgtttta 21420
cggcagtgag agcagagata gcgctgatgt ccggcggtgc ttttgccgtt acgcaccacc 21480
ccgtcagtag ctgaacagga gggacagctg atagaaacag aagccactgg agcacctcaa 21540
aaacaccatc atacactaaa tcagtaagtt ggcagcatca cccctggttg gcttggtttc 21600
atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc 21660
aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct 21720
cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa 21780
gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga 21840
aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaaagatcc 21890
<210> 154
<211> 22029
<212> DNA
<213> 人工序列
<220>
<223> 转化构建体
<400> 154
gtcgacgatc gtcaacgttc acttctaaag aaatagcgcc actcagcttc ctcagcggct 60
ttatccagcg atttcctatt atgtcggcat agttctcaag atcgacagcc tgtcacggtt 120
aagcgagaaa tgaataagaa ggctgataat tcggatctct gcgagggaga tgatatttga 180
tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc 240
cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca 300
aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg 360
tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca 420
ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt 480
ttttaatgta ctgaattcca atccccaaaa atctgagctt aacagcacag ttgctcctct 540
cagagcagaa tcgggtattc aacaccctca tatcaactac tacgttgtgt ataacggtcc 600
acatgccggt atatacgatg actggggttg tacaaaggcg gcaacaaacg gcgttcccgg 660
agttgcacac aagaaatttg ccactattac agaggcaaga gcagcagctg acgcgtacac 720
aacaagtcag caaacagaca ggttgaactt catccccaaa ggagaagctc aactcaagcc 780
caagagcttt gctaaggccc taacaagccc accaaagcaa aaagcccact ggctcacgct 840
aggaaccaaa aggcccagca gtgatccagc cccaaaagag atctcctttg ccccggagat 900
tacaatggac gatttcctct atctttacga tctaggaagg aagttcgaag gtgaaggtga 960
cgacactatg ttcaccactg ataatgagaa ggttagcctc ttcaatttca gaaagaatgc 1020
tgacccacag atggttagag aggcctacgc agcaggtctc atcaagacga tctacccgag 1080
taacaatctc caggagatca aataccttcc caagaaggtt aaagatgcag tcaaaagatt 1140
caggactaat tgcatcaaga acacagagaa agacatattt ctcaagatca gaagtactat 1200
tccagtatgg acgattcaag gcttgcttca taaaccaagg caagtaatag agattggagt 1260
ctctaaaaag gtagttccta ctgaatctaa ggccatgcat ggagtctaag attcaaatcg 1320
aggatctaac agaactcgcc gtgaagactg gcgaacagtt catacagagt cttttacgac 1380
tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca cgacactctg gtctactcca 1440
aaaatgtcaa agatacagtc tcagaagacc aaagggctat tgagactttt caacaaagga 1500
taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc atcgaaagga 1560
cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggctatca 1620
ttcaagatct ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg 1680
tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gacatctcca 1740
ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc tctatataag 1800
gaagttcatt tcatttggag aggacacgct cgagtataag agctctattt ttacaacaat 1860
taccaacaac aacaaacaac aaacaacatt acaattacta tttacaatta ccatgagccc 1920
agaacgacgc ccggccgaca tccgccgtgc caccgaggcg gacatgccgg cggtctgcac 1980
catcgtcaac cactacatcg agacaagcac ggtcaacttc cgtaccgagc cgcaggaacc 2040
gcaggagtgg acggacgacc tcgtccgtct gcgggagcgc tatccctggc tcgtcgccga 2100
ggtggacggc gaggtcgccg gcatcgccta cgcgggcccc tggaaggcac gcaacgccta 2160
cgactggacg gccgagtcaa ccgtgtacgt ctccccccgc caccagcgga cgggactggg 2220
ctccacgctc tacacccacc tgctgaagtc cctggaggca cagggcttca agagcgtggt 2280
cgctgtcatc gggctgccca acgacccgag cgtgcgcatg cacgaggcgc tcggatatgc 2340
cccccgcggc atgctgcggg cggccggctt caagcacggg aactggcatg acgtgggttt 2400
ctggcagctg gacttcagcc tgccggtacc gccccgtccg gtcctgcccg tcaccgagat 2460
ttgatgaccc gggggatcct agagtcctgc tttaatgaga tatgcgagac gcctatgatc 2520
gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct 2580
cagatcctta ccgccggttt cggttcattc taatgaatat atcacccgtt actatcgtat 2640
ttttatgaat aatattctcc gttcaattta ctgattgtac cctactactt atatgtacaa 2700
tattaaaatg aaaacaatat attgtgctga ataggtttat agcgacatct atgatagagc 2760
gccacaataa caaacaattg cgttttatta ttacaaatcc aattttaaaa aaagcggcag 2820
aaccggtcaa acctaaaaga ctgattacat aaatcttatt caaatttcaa aagtgcccca 2880
ggggctagta tctacgacac accgagcggc gaactaataa cgctcactga agggaactcc 2940
ggttccccgc cggcgcgcat gggtgagatt ccttgaagtt gagtattggc cgtccgctct 3000
accgaaagtt acgggcacca ttcaacccgg tccagcacgg cggccgggta accgacttgc 3060
tgccccgaga attatgcagc atttttttgg tgtatgtggg ccccaaatga agtgcaggtc 3120
aaaccttgac agtgacgaca aatcgttggg cgggtccagg gcgaattttg cgacaacatg 3180
tcgaggctca gcaggacctg caggcatgca agctctagag cggccgcttt aagagtgttg 3240
aggatgtgct tacttaaatt tcaaaagggc tatttaggga taatatttgg tgaagcatgg 3300
ctggtggact gaccataggt acccactgaa taaacatatc agcccaaagg cagagggtat 3360
ccctccctgt aatacgtact agtattcgag gagcgtgcta aagtagtatc ctctcttgat 3420
gctcctcgtc tccctccact gagtactccc ttcctctcct cattctcatc tcagtcctct 3480
cctcctccct cgaataagat agatccgccc ttgttgctga tctcatctcg cctcctattg 3540
gcctcgcccc tgccaactga ctgctctatt ggtgaccgtg gactttttgg gtatatatat 3600
ttcctgtcga tttgtttcca cttaaagtag caatgcttgg atctaaaact tattacttct 3660
cctgcttcag attctgatcc atttgtgttg attgaactca gggtgggatc gtggggctct 3720
gtatctatct aggatggata ttccaagcaa tttactctct tgagtgctgt tggtcttcct 3780
ctgctcctcg tctccgcttc ctctcagtct cctccattgc gctgcctgga acagatcgat 3840
ccgccttgtt gctgatctca tccatcctcc gtggagattt cgggtggcag tttccagtga 3900
ctattcagag caccatacta gtggcctggt aaaaatttcc ctcccatctt tcttgctatt 3960
ctcttcttat taatctgctg atttgcttga tggtaagttc ttacttattc ttccagatcg 4020
agtgaagcga catccgccgc cgtcttgtcc cctacgacgc cgctgtgttc tgctctgtcc 4080
tcctctgccg gtgaagcatc aacggtgacg gccatccggt gttgacgccg cggctgtgtt 4140
ctcctctgcc gtgaagtatc aaaggtgggt ttggatggat atcacagttt ggattggcaa 4200
ctctgttcta tagctcgttg aaattaaagc ttcacacagt tttgattgga ttacagctcc 4260
ccatattcat ctattttgca ccatgcatcg aatccactga cctccacacg ctcacggatc 4320
tggcctctgg tgcccacacg ctcacggatc cgacagagtg ctcccttcct atcatctcag 4380
tcctcatctc ctggtcgatc cggcttcttg tttggtgagc tcgtccaacc tccgagctcg 4440
cccctgacac ctgtggagtg gaggtatatt tctttccagc tgaagtacgc ttggatctaa 4500
aaacttacgt ctcctgcttc actgcttgct tgggagtctg atcgatttgt gttggttgaa 4560
tcatttgggg tggcagtgga gcagctagca agaatcatgg agtttccaat cattattcag 4620
agcaccacgc atggaatcca ctgacctggt aactttgtct catctttctt gctactgaaa 4680
tcttccacat ccccaccttc cttgttcatc tgctgatttc ccagattata cttccagatt 4740
gagtgaagcg ccgtcttgtc ccctcacgga tctggcctct ggtgcccagc cgccgctttc 4800
gcttcgttgt ccgacgaggc caccgccgct gtgttctgct ctgccgtgaa gcacaaaaag 4860
gtggggtctt cctctgctct ttctccactg acggcgatcc gaccttgaca cccgtggccg 4920
tggcagtggg gtcttcctct gctctgtttt tttaccagtt attggatcta aaaagggatc 4980
taaaagttat tacttctcct gcttgagatt ctgaccgatt tgtgttgatt gaatccaggg 5040
tggggtcgtg gggctctgta tctatctagt acggagtacc actgtattcc aagcaattta 5100
ctctcttgac tgctgttgga cttcctctgt ccccgcttcc attgtgctgc cttgaacaga 5160
tccatccacc ttgttgctaa tctcatccaa cctccgggta tgtttcttgt cgatttgttt 5220
ccagtaaagt agcaatgctt gggtctaaaa agtcattgct tctcctgctt gacgagtctc 5280
attgatttgc ccagtcatta ttcagagcac catgcatcca aggcgctgac ccggctataa 5340
gtttttatta cagggccgcc gtgttgtccc catcgagcaa agcgtttaat ccactgaccc 5400
gcttcgttgt ccgcacagcg tgaccatccg acgaagacgc cgccgttgtg ttctgctctc 5460
ctctgaagca tcaaaaggtg ggcttggtcc agatccccac attcatcgat ttggcttgcc 5520
aactcagttc caccagagct tggattgtgt tacagatccc taaattcatc gatttgcagg 5580
tttcagctga ttgatcaaga gagctctcaa tgatggcgga agttctggtg agcgcatcca 5640
caggggcaat gggttccctc ctgcggaagt tgggagctat gttgacggat gaatacaagc 5700
tgctcaagaa cgtccgtggg gacatcaagt tcctcaagga cgagctggag gtcatgtgcg 5760
cgttcctcct caagatgtca gatgtggagg agcctgacga accaacaaag ctgcgtgtga 5820
cggcggtgcg ggagatgtcc tataagatag aggacaacat cgacaagttc atggtcctcg 5880
tggaacagga acacgggtcc tcctgttccg aggcggctca tggcgtcgcc aagctcatgg 5940
acaagtgcaa gaacttgctg ccggacatca aggcccgccg caggatcgcc aaggaggtca 6000
aagacatcaa gaaagaaatt aaggatgtca gcgacagatt ttcaaggtac cagatcttgt 6060
tctccttaca gtactattag tacctaccat cagatttgtg accgatttgt tgccactcaa 6120
ggtacaagat tgacgactcc tcctcctcta tgccggcaaa agacaaggtc gaccctcgac 6180
ttcgtgcagt ctacaaagac gccgcagagc tcgtcggaat tgatggacca aaggatgaac 6240
ttgtcaagtg gctgaatgag aaagaaggcc aatcactgaa atctgtctct attgttggat 6300
atggagggtt gggcaagacc acgctcgcca accagatccg ggtcaacctt ggagcgacct 6360
tcgactgcgg ggcttttgtc tcgatttcac gcaagcctga catgaaggcg atattaagat 6420
ctatattatc acaaatcacc aagaaagacg atgcttgctc tagattagat gatatacaac 6480
tcatcatcga caagattcga gaattcctcc aagacacaag gtatgtatgt gagatctgct 6540
acgaagtaac attgcttaat tagctcatgc ataatcggtt gcctagaatt ttgctaatga 6600
agattgcttt gtgtgattta ctttcgtcct caacttgtat gactgcgctt aaactttcaa 6660
gtcattgttt cttgcaactt atgtcgatct gttcactaat taagatatag ctaaaatgtg 6720
tttgccgatt tctgtcgcca cacattttct aatttgattg ccacaagttt gttaaacttg 6780
cggctatgtt ctgtttatta aataattgtg acatggcgtc agtcaaaaca ataaagtaca 6840
tgcaattccc tctcttttgt acaagataaa tatccgcacg cactacatga ctgcaaatat 6900
atctatcttc taaggaccca aagcacttgc atgtgcagac cattgttacc ggatatgcag 6960
tacattaggt ttgtgattag ccactttaag aacatagggt ttctagaggc tataaaccta 7020
cggttatcag tattataatc ttgaatggat atcaaacatc cgttgaagaa tctatgccca 7080
taacacaaaa agagaaaagg gaagaattaa tcatagtcag atcggaccag cctgactagc 7140
gtaacataat gacaacagta ggttgttgcg tctttcctgg tttcgtagca atttgcatga 7200
cgaattgagg cgcagagcca ccactcaata tgtggtccta ccaatcatga ggaaataact 7260
tgttaccaaa atgacaaaag aaaataggta gtccccatca ttgtttcaat atctatcctc 7320
ttgcgaacat gctattggca gtggcaaaac attgtacctt gccactggca acagctgcct 7380
ttactcggcc tgagcattca tatttttttc ctcttggttg ttaaatcttg ctatttttcg 7440
cttgccttct atggcaatct ttttttttct tatattgcca ccaaattgta tctaagagca 7500
actccaacac gccgacgcaa tttttgtcca tttagatcgg ccaagcggac ggggtgtctg 7560
gttttgcctt tgggtggtgc gcctacgcat tagtccgcgt gggcatttaa aaaacttaaa 7620
aagcttaaaa taattaaata taaataaaaa tgcatattaa agcccgtcaa agtccacata 7680
attaaacata atacattaat atattaataa aaaacagccg ccccagcccg cagccctagg 7740
cgtcttcgtc ggaggagtgg gtgaggtcga agaatgccgg cagctcagcc cacgggaagg 7800
cctgctggtg ggcagccagc accgcctgct ccgctgtctg cggcggcggc ggccctacct 7860
ggcaccgcgc acgcctcctc ctcgcgctcc tgccgctcct cctcctcatc gcacgcccaa 7920
cgaagttttg gcgctcctcc ccctcgggtt cccgctgcat gcggacctgg ccgtcgtacc 7980
gctcctcctc cagcgtgcgg tccttccacg ccacgaggta ggccgcctcc tcctccatca 8040
cgcacagcca aatgggcggc ttgctcatcc gcttgcagac gatgccctgc cattggtaaa 8100
cgccggtggg cgcgggtggt gctagcgctg gtgcaggcgg tgacgatgtg gctggtggtc 8160
ccggcaggta gacactatag ctggcggccg atagctgcag cgtgtcatcg aggccagacc 8220
actaggagtc ctcgcactaa gactcctcgg tgacgtgatg ggccccctcc tccaatgcct 8280
tgaggaacgc cgcctcctcc tcaacctact atttgaccac cggtggcggc ggtggagggc 8340
aggcttcgag ctggacggcg tcgcggaggc gcacatggtg ctcgatggtg aaccagactt 8400
ctcagttagg ggagtctggc ctcgatgggt ggagtcgcga cgctgctctg gcgtcaggag 8460
agcacgccgg gcccgaatct cgttggcgcg cgcacgctcg gactgtggca ccgccggcac 8520
tggaatgtgg tgggggtcga ggtgccagcc gtgggggaag ttcacgtccg gccacggcag 8580
tgagacgcgg tactcccggt ggtactaact acgcgtggtg caccgggatg tactggtggt 8640
cccgtggcgc tgccgccctc ccatcccgca gcgccgacac caatgcgaac cccttcgctg 8700
ccggcctcac cggcccttgc aacgagccgg cctcgtggtc gtgcttgcct tgcccttacc 8760
tttgccaaag aaacccatga cggctagggt tttctgtcac cggcgaggga gcagatgtgg 8820
actagagtgg gaggagaagt ggatgaggcc cgccccgccg cacccttgca attaaaaagg 8880
gcgtgatccg gacgtttcta gtcactgcca tgcgggcccg aggtaggtgg gcgccattga 8940
ctacgtctgt ggtggtagtt ggctggcaga cacgccagcc cgaggcgcac accgagcgga 9000
cgcgcaacac gtccgtctcg tgttcgtgtg aacgcaaatc aggcacaaat ttgggctgga 9060
aatgcgtcta catggatgcc gaacggacgt gatttgggtt tgtgtccgca cgttgggccg 9120
gcgtttttgt gcctttcgac cctaatggac acacacggac caaatccgtt ggcgtgttag 9180
agttgcttgt acctagtact actatgtgtg gagttgttat ctttgagttt ctcccccatc 9240
tgtagctgtg tattattgtg agatgctctc aggatttcac ttattttaga taatgacaac 9300
tttcttgatg ctagacaatt acatcaaggt gtattatatc taggatgcac acagatgtga 9360
gattgtttca ggattttagg ttacttggta ggtgttacta taaatatcta atgagctcta 9420
atgtgtatgt tcatgaaact gtgttgcagg tactttatca taattgatga tatatgggag 9480
ttaggaacat gggaaacttt gaaatgtgca tttgtcaaga atacattggg cagcagaata 9540
attatcacaa cacgtatagt tgatgttgcc aaatcttgtt ctccttctag tgaagatctt 9600
gtctatgaga tgaaaccact cagtgaggct gactcgaaga aattgttttt caagaggata 9660
tttggctgtg aagaaagctg tcctgatagc ttgaaagaag ctgccaatga tattttgaaa 9720
aaatgccgtg gcctacctct ggccatcaat gccatttcta gcgtgttggt cacaacaagg 9780
gaaacaaaag aagagtggga tcgggtgcga cattcaatac gctcttcaaa ggtcaaaagt 9840
gatataatag aaaccatgaa ttacatattg tctcttagtt attttgatct tccccaccac 9900
ctaagaagct gcttattgta cctggctctg tttcctgaag accaattgat tggaaggaag 9960
cggttagtac gcagatggat ttctgaaggc tttatccatg gagaaagtgg acaagatctc 10020
atggaattag gagaggaata ttttcaccag cttgtgaaca gaagtctaat acagcccggc 10080
aacatagggt atgacggcaa ggcaatgtat tgccgagtcc atgacaccat ccttgatttc 10140
ctaattgaca aatcctctga agagaacatg tgtactgtcc taaagaaaca atgcaagcct 10200
aatggcatag ttcgtcggct ctctctaatg gggaatgagg atgaagaaat cgtggagcaa 10260
ttggatctgt cacacgctcg gtcaatcact gcttttgggg atatcaagct attgccttcg 10320
cttgggaggt caaaatgctt gcgcgtgcta gacttgcaag actgcgatca gttggaaaat 10380
catcatatca aggatattga aagactctat cagctaagat acttggatat ctcttctaca 10440
ggaatcacgg agcttcccag gcaaattggg gagttgctgt atctagagac gctagttgcc 10500
tatggattac gtgagcttcc tgaaagcaca agtcggctac aacgactggc acgtttgttt 10560
gtttactctg gttgtaagct tccaggtggg ttgggaaact tgataaactt gcaagagctt 10620
gactgcgttg atgccttgca tttgaagcat gtggaagagc tcggcaaact aacaaatctg 10680
agaaagctga gtattaaatt ggacactggt gggattgaag gcaacaaatt agaagagtcc 10740
aaggaaaagc tggtgtcctc tctctgtaaa ctggacgaat gcggcctcct ttccctgagt 10800
attgattatt atctgagaga aaaggatgga gaagagccgt tcctccctgc tttgggttgt 10860
atccaggagg tttttgtata tggacaagat atcagccgga ttagcagatg gctcgcgtca 10920
cttcccaacc tacacaggtt gcttttggac gatccgaaga tagagcagca ggatattgag 10980
atgattggat tgatacccaa tctgatcgat ttgacactgc cgcctctata caaaacagac 11040
gacgctgggc ggctcatcat caggcgtgaa ggatttcaac agttgcagaa gtttgaggct 11100
tacaatactc gtatgggggt tttaatgttt gaaccggggg ctatgccgag gctcaaggag 11160
cttaaactcc ataacttcat cgaaaagccc aaatctgctg cggtcgattt cgactttggc 11220
atccaacgcc tctccagcct tgctcgcctc actgtcagct tatcctgcgg tggctggacg 11280
gtggcagaag tagaggctgc ggaggatgct ttcaagagca tggccgaggc gaaccccaac 11340
cgtccaatac tggagatgac aagatataat acgcagcata tgttacaaga cgagcaaata 11400
ggtatgacag gctcaggtac tattaactag tttgtacttt tgtcttggtg agcagtagat 11460
caaaagatgt gcatatgctt caatcttttt tccggtaatc tatttcagct acaacgccgg 11520
ccgtcaagtc ctaggctttg cctaaaccga gatgcgcctt tggttatgga gcccaagtca 11580
cgtactcgga ttggatgctg cagcaaatga tacttctgta catgtctgca agtatcacga 11640
attagtggaa tgactaggaa taagtaagac aagtatcacg aattcagagt cggatgcaat 11700
ttttttcttt tcttttgggc ataagtagat tgtcatttga gtattttttt ccattaatat 11760
ttgtgtattc ttagattgag actttgatat gtaacccaga tttctgttgt taggaaacac 11820
ataccgtgta gcttatcaga agaaaaagat ctagcgtgca tctatattct atcggactat 11880
gatatttggc acatgtgtgc catataaaca aaactgtcac acctctattt ccttcactct 11940
aaatagatgt atacattagt ttgattcttt cttctttcac gtgcatttct ccttctaatt 12000
agatgcatac attagaggac aaaaaagacg atgtcattct agattttctt tattaaaata 12060
tttaaaatgt tttgtagctc aaaccgtcgg tccgatgtaa aaaccgtttt tacatatacg 12120
attcgtcgcg atgagatctt tgaaactaga tcctatgttg acatgtttcg atgacttttt 12180
ttaaccaaaa tttaccatgc cttttatatg caagttaccg tgctatttcc acataagtca 12240
tcggaggcat aaaaatggtt caccgacact acagttcata tattaccatg atgtttacat 12300
ataagttacc gggagggggg gggggggtgt ctgttttcaa taatttcccc accgagtcaa 12360
atattaccat agtatatgta cgtaagttat caggtatccg atgggtatat taccatggct 12420
atcttttaaa aaaatctcta atttaccgcg gtgtttgtat ctaaattatc aagtttgtgg 12480
tgcctaattt actatgctat ttacatacaa aagttaccag tgtatttttt gacaattttt 12540
tactattctt ccccaaatca agactattac cgtgctgttt acacacaagt taccgggtat 12600
attttcaaca acttcatcca cccgaatcaa acttatcatg atgtttgtga gtaacttatc 12660
atgtctgcaa tgcgtatatt accattctat ttaaacaaaa tttaccaacg atatgcttca 12720
aacaattttt tacgtgggtc aaagctagca tactgttttt gcgtaagtta ttgtatctgc 12780
aataaatata ttaccgtgct atttacctag aagttattgg gggtaatgtt cttcaacgaa 12840
aaaaaattca gggtcaaaaa attattgtgg tgtttgaacg agagttatta gctctccgat 12900
gggtaaatta tcatgttatt tgcacataaa ttaccgaggg tatgcttttc aacaacttcc 12960
tccatccagg tcaaagttat catggtgttt ttgctcaagt tttcaagttt gtggtgcgta 13020
tattaccacg atgttgactg tttacaaggt ttttgagtcg ccgactaatc gccgactaat 13080
cgtcgagtcg tcgcggcggc ggccgccacc gcggctgagt ggctccttca agcttgcgga 13140
tccgggcgga aataggtaaa gaagttgcgg ataaggtaat tgccattgca gattatttgg 13200
attgagagtg aatatgagac tctaattgga taccgagggg aatttatgga acgtcagtgg 13260
agcatttttg acaagaaata tttgctagct gatagtgacc ttaggcgact tttgaacgcg 13320
caataatggt ttctgacgta tgtgcttagc tcattaaact ccagaaaccc gcggctgagt 13380
ggctccttca acgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt cccgcgtcat 13440
cggcgggggt cataacgtga ctcccttaat tctccgctca tgatcagatt gtcgtttccc 13500
gccttcagtt taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa 13560
agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc 13620
catttgtatg tgcatgccaa ccacagggtt cccctcggga gtgcttggca ttccgtgcga 13680
taatgacttc tgttcaacca cccaaacgtc ggaaagcctg acgacggagc agcattccaa 13740
aaagatccct tggctcgtct gggtcggcta gaaggtcgag tgggctgctg tggcttgatc 13800
cctcaacgcg gtcgcggacg tagcgcagcg ccgaaaaatc ctcgatcgca aatccgacgc 13860
tgtcgaaaag cgtgatctgc ttgtcgctct ttcggccgac gtcctggcca gtcatcacgc 13920
gccaaagttc cgtcacagga tgatctggcg cgagttgctg gatctcgcct tcaatccggg 13980
tctgtggcgg gaactccacg aaaatatccg aacgcagcaa gatcgtcgac ccctttccga 14040
cgctcaccgg gctggttgcc ctcgccgctg ggctggcggc cgtctatggc cctgcaaacg 14100
cgccagaaac gccgtcgaag ccgtgtgcga gacaccgcgg ccggccgccg gcgttgtgga 14160
tacctcgcgg aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg 14220
gccgactcac ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg 14280
tggagctggc cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag 14340
atgatgtgga caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact 14400
actgacagat gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg 14460
gcgcacctat tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt 14520
ttccgcccgt ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt 14580
ataaaccttg tttttaacca gggctgcgcc ctggcgcgtg accgcgcacg ccgaaggggg 14640
gtgccccccc ttctcgaacc ctcccggccc gctaacgcgg gcctcccatc cccccagggg 14700
ctgcgcccct cggccgcgaa cggcctcacc ccaaaaatgg cagccaagct cctaacattt 14760
tattagagag caggctagtt gcttagatac atgatcttca ggccgttatc tgtcagggca 14820
agcgaaaatt ggccatttat gacgaccaat gccccgcaga agctcccatc tttgccgcca 14880
tagacgccgc gccccccttt tggggtgtag aacatccttt tgccagatgt ggaaaagaag 14940
ttcgttgtcc cattgttggc aatgacgtag tagccggcga aagtgcgaga cccatttgcg 15000
ctatatataa gcctacgatt tccgttgcga ctattgtcgt aattggatga actattatcg 15060
tagttgctct cagagttgtc gtaatttgat ggactattgt cgtaattgct tatggagttg 15120
tcgtagttgc ttggagaaat gtcgtagttg gatggggagt agtcataggg aagacgagct 15180
tcatccacta aaacaattgg caggtcagca agtgcctgcc ccgatgccat cgcaagtacg 15240
aggcttagaa ccaccttcaa cagatcgcgc atagtcttcc ccagctctct aacgcttgag 15300
ttaagccgcg ccgcgaagcg gcgtcggctt gaacgaattg ttagacatta tttgccgact 15360
accttggtga tctcgccttt cacgtagtga acaaattctt ccaactgatc tgcgcgcgag 15420
gccaagcgat cttcttgtcc aagataagcc tgcctagctt caagtatgac gggctgatac 15480
tgggccggca ggcgctccat tgcccagtcg gcagcgacat ccttcggcgc gattttgccg 15540
gttactgcgc tgtaccaaat gcgggacaac gtaagcacta catttcgctc atcgccagcc 15600
cagtcgggcg gcgagttcca tagcgttaag gtttcattta gcgcctcaaa tagatcctgt 15660
tcaggaaccg gatcaaagag ttcctccgcc gctggaccta ccaaggcaac gctatgttct 15720
cttgcttttg tcagcaagat agccagatca atgtcgatcg tggctggctc gaagatacct 15780
gcaagaatgt cattgcgctg ccattctcca aattgcagtt cgcgcttagc tggataacgc 15840
cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag aatctcgctc 15900
tctccagggg aagccgaagt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca 15960
tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca 16020
tccactgcgg agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg 16080
acgccaacta cctctgatag ttgagtcgat acttcggcga tcaccgcttc cctcatgatg 16140
tttaactcct gaattaagcc gcgccgcgaa gcggtgtcgg cttgaatgaa ttgttaggcg 16200
tcatcctgtg ctcccgagaa ccagtaccag tacatcgctg tttcgttcga gacttgaggt 16260
ctagttttat acgtgaacag gtcaatgccg ccgagagtaa agccacattt tgcgtacaaa 16320
ttgcaggcag gtacattgtt cgtttgtgtc tctaatcgta tgccaaggag ctgtctgctt 16380
agtgcccact ttttcgcaaa ttcgatgaga ctgtgcgcga ctcctttgcc tcggtgcgtg 16440
tgcgacacaa caatgtgttc gatagaggct agatcgttcc atgttgagtt gagttcaatc 16500
ttcccgacaa gctcttggtc gatgaatgcg ccatagcaag cagagtcttc atcagagtca 16560
tcatccgaga tgtaatcctt ccggtagggg ctcacacttc tggtagatag ttcaaagcct 16620
tggtcggata ggtgcacatc gaacacttca cgaacaatga aatggttctc agcatccaat 16680
gtttccgcca cctgctcagg gatcaccgaa atcttcatat gacgcctaac gcctggcaca 16740
gcggatcgca aacctggcgc ggcttttggc acaaaaggcg tgacaggttt gcgaatccgt 16800
tgctgccact tgtttaatag actggatgga ggcggataaa gttgcaggac cacttctgcg 16860
ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc 16920
tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta 16980
cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc 17040
ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga 17100
tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 17160
gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat 17220
caaaggatct tcttgatatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 17280
accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 17340
ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt 17400
aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 17460
accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 17520
gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 17580
ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac 17640
gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 17700
gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 17760
ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 17820
aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat 17880
gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc 17940
tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga 18000
agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg 18060
aagatcggcg gcaatagctt cttagcgcca tcccggctga gaaagcccag taaggaaaca 18120
actgtaggtt cgagtcgcga gatcccccgg aaccaaagga agtaggttaa acccgctccg 18180
atcaggccga gccacgccag gccgagaaca ttggttcctg taggcatcgg gattggcgga 18240
tcaaacacta aagctactgg aacgagcaga agtcctccgg ccgccagttg ccaggcggta 18300
aaggtgagca gaggcacggg aggttgccac ttgcgggtca gcacggttcc gaacgccatg 18360
gaaaccgccc ccgccaggcc cgctgcgacg ccgacaggat ctagcgctgc gtttggtgtc 18420
aacaccaaca gcgccacgcc cgcagttccg caaatagccc ccaggaccgc catcaatcgt 18480
atcgggctac ctagcagagc ggcagagatg aacacgacca tcagcggctg cacagcgcct 18540
accgtcgccg cgaccccgcc cggcaggcgg tagaccgaaa taaacaacaa gctccagaat 18600
agcgaaatat taagtgcgcc gaggatgaag atgcgcatcc accagattcc cgttggaatc 18660
tgtcggacga tcatcacgag caataaaccc gccggcaacg cccgcagcag cataccggcg 18720
acccctcggc ctcgctgttc gggctccacg aaaacgccgg acagatgcgc cttgtgagcg 18780
tccttggggc cgtcctcctg tttgaagacc gacagcccaa tgatctcgcc gtcgatgtag 18840
gcgccgaatg ccacggcatc tcgcaaccgt tcagcgaacg cctccatggg ctttttctcc 18900
tcgtgctcgt aaacggaccc gaacatctct ggagctttct tcagggccga caatcggatc 18960
tcgcggaaat cctgcacgtc ggccgctcca agccgtcgaa tctgagcctt aatcacaatt 19020
gtcaatttta atcctctgtt tatcggcagt tcgtagagcg cgccgtgcgt cccgagcgat 19080
actgagcgaa gcaagtgcgt cgagcagtgc ccgcttgttc ctgaaatgcc agtaaagcgc 19140
tggctgctga acccccagcc ggaactgacc ccacaaggcc ctagcgtttg caatgcacca 19200
ggtcatcatt gacccaggcg tgttccacca ggccgctgcc tcgcaactct tcgcaggctt 19260
cgccgacctg ctcgcgccac ttcttcacgc gggtggaatc cgatccgcac atgaggcgga 19320
aggtttccag cttgagcggg tacggctccc ggtgcgagct gaaatagtcg aacatccgtc 19380
gggccgtcgg cgacagcttg cggtacttct cccatatgaa tttcgtgtag tggtcgccag 19440
caaacagcac gacgatttcc tcgtcgatca ggacctggca acgggacgtt ttcttgccac 19500
ggtccaggac gcggaagcgg tgcagcagcg acaccgattc caggtgccca acgcggtcgg 19560
acgtgaagcc catcgccgtc gcctgtaggc gcgacaggca ttcctcggcc ttcgtgtaat 19620
accggccatt gatcgaccag cccaggtcct ggcaaagctc gtagaacgtg aaggtgatcg 19680
gctcgccgat aggggtgcgc ttcgcgtact ccaacacctg ctgccacacc agttcgtcat 19740
cgtcggcccg cagctcgacg ccggtgtagg tgatcttcac gtccttgttg acgtggaaaa 19800
tgaccttgtt ttgcagcgcc tcgcgcggga ttttcttgtt gcgcgtggtg aacagggcag 19860
agcgggccgt gtcgtttggc atcgctcgca tcgtgtccgg ccacggcgca atatcgaaca 19920
aggaaagctg catttccttg atctgctgct tcgtgtgttt cagcaacgcg gcctgcttgg 19980
cctcgctgac ctgttttgcc aggtcctcgc cggcggtttt tcgcttcttg gtcgtcatag 20040
ttcctcgcgt gtcgatggtc atcgacttcg ccaaacctgc cgcctcctgt tcgagacgac 20100
gcgaacgctc cacggcggcc gatggcgcgg gcagggcagg gggagccagt tgcacgctgt 20160
cgcgctcgat cttggccgta gcttgctgga ccatcgagcc gacggactgg aaggtttcgc 20220
ggggcgcacg catgacggtg cggcttgcga tggtttcggc atcctcggcg gaaaaccccg 20280
cgtcgatcag ttcttgcctg tatgccttcc ggtcaaacgt ccgattcatt caccctcctt 20340
gcgggattgc cccgactcac gccggggcaa tgtgccctta ttcctgattt gacccgcctg 20400
gtgccttggt gtccagataa tccaccttat cggcaatgaa gtcggtcccg tagaccgtct 20460
ggccgtcctt ctcgtacttg gtattccgaa tcttgccctg cacgaatacc agctccgcga 20520
agtcgctctt cttgatggag cgcatgggga cgtgcttggc aatcacgcgc accccccggc 20580
cgttttagcg gctaaaaaag tcatggctct gccctcgggc ggaccacgcc catcatgacc 20640
ttgccaagct cgtcctgctt ctcttcgatc ttcgccagca gggcgaggat cgtggcatca 20700
ccgaaccgcg ccgtgcgcgg gtcgtcggtg agccagagtt tcagcaggcc gcccaggcgg 20760
cccaggtcgc cattgatgcg ggccagctcg cggacgtgct catagtccac gacgcccgtg 20820
attttgtagc cctggccgac ggccagcagg taggccgaca ggctcatgcc ggccgccgcc 20880
gccttttcct caatcgctct tcgttcgtct ggaaggcagt acaccttgat aggtgggctg 20940
cccttcctgg ttgggtaatg actccaactt attgatagtg ttttatgttc agataatgcc 21000
cgatgacttt gtcatgcagc tccaccgatt ttgagaacga cagcgacttc cgtcccagcc 21060
gtgccaggtg ctgcctcaga ttcaggttat gccgctcaat tcgctgcgta tatcgcttgc 21120
tgattacgtg cagctttccc ttcaggcggg attcatacag cggccagcca tccgtcatcc 21180
atatcaccac gtcaaagggt gacagcaggc tcataagacg ccccagcgtc gccatagtgc 21240
gttcaccgaa tacgtgcgca acaaccgtct tccggagact gtcatacgcg taaaacagcc 21300
agcgctggcg cgatttagcc ccgacatagc cccactgttc gtccatttcc gcgcagacga 21360
tgacgtcact gcccggctgt atgcgcgagg ttaccgactg cggcctgagt tttttaagtg 21420
acgtaaaatc gtgttgaggc caacgcccat aatgcgggct gttgcccggc atccaacgcc 21480
attcatggcc atatcaatga ttttctggtg cgtaccgggt tgagaagcgg tgtaagtgaa 21540
ctgcagttgc catgttttac ggcagtgaga gcagagatag cgctgatgtc cggcggtgct 21600
tttgccgtta cgcaccaccc cgtcagtagc tgaacaggag ggacagctga tagaaacaga 21660
agccactgga gcacctcaaa aacaccatca tacactaaat cagtaagttg gcagcatcac 21720
ccctggttgg cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag 21780
ccggccagcc tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag 21840
tgaagaagga acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc 21900
gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga 21960
aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg 22020
aaaagatcc 22029
<210> 155
<211> 21880
<212> DNA
<213> 人工序列
<220>
<223> 转化构建体
<400> 155
gtcgacgatc gtcaacgttc acttctaaag aaatagcgcc actcagcttc ctcagcggct 60
ttatccagcg atttcctatt atgtcggcat agttctcaag atcgacagcc tgtcacggtt 120
aagcgagaaa tgaataagaa ggctgataat tcggatctct gcgagggaga tgatatttga 180
tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc 240
cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca 300
aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg 360
tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca 420
ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt 480
ttttaatgta ctgaattcca atccccaaaa atctgagctt aacagcacag ttgctcctct 540
cagagcagaa tcgggtattc aacaccctca tatcaactac tacgttgtgt ataacggtcc 600
acatgccggt atatacgatg actggggttg tacaaaggcg gcaacaaacg gcgttcccgg 660
agttgcacac aagaaatttg ccactattac agaggcaaga gcagcagctg acgcgtacac 720
aacaagtcag caaacagaca ggttgaactt catccccaaa ggagaagctc aactcaagcc 780
caagagcttt gctaaggccc taacaagccc accaaagcaa aaagcccact ggctcacgct 840
aggaaccaaa aggcccagca gtgatccagc cccaaaagag atctcctttg ccccggagat 900
tacaatggac gatttcctct atctttacga tctaggaagg aagttcgaag gtgaaggtga 960
cgacactatg ttcaccactg ataatgagaa ggttagcctc ttcaatttca gaaagaatgc 1020
tgacccacag atggttagag aggcctacgc agcaggtctc atcaagacga tctacccgag 1080
taacaatctc caggagatca aataccttcc caagaaggtt aaagatgcag tcaaaagatt 1140
caggactaat tgcatcaaga acacagagaa agacatattt ctcaagatca gaagtactat 1200
tccagtatgg acgattcaag gcttgcttca taaaccaagg caagtaatag agattggagt 1260
ctctaaaaag gtagttccta ctgaatctaa ggccatgcat ggagtctaag attcaaatcg 1320
aggatctaac agaactcgcc gtgaagactg gcgaacagtt catacagagt cttttacgac 1380
tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca cgacactctg gtctactcca 1440
aaaatgtcaa agatacagtc tcagaagacc aaagggctat tgagactttt caacaaagga 1500
taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc atcgaaagga 1560
cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggctatca 1620
ttcaagatct ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg 1680
tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gacatctcca 1740
ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc tctatataag 1800
gaagttcatt tcatttggag aggacacgct cgagtataag agctctattt ttacaacaat 1860
taccaacaac aacaaacaac aaacaacatt acaattacta tttacaatta ccatgagccc 1920
agaacgacgc ccggccgaca tccgccgtgc caccgaggcg gacatgccgg cggtctgcac 1980
catcgtcaac cactacatcg agacaagcac ggtcaacttc cgtaccgagc cgcaggaacc 2040
gcaggagtgg acggacgacc tcgtccgtct gcgggagcgc tatccctggc tcgtcgccga 2100
ggtggacggc gaggtcgccg gcatcgccta cgcgggcccc tggaaggcac gcaacgccta 2160
cgactggacg gccgagtcaa ccgtgtacgt ctccccccgc caccagcgga cgggactggg 2220
ctccacgctc tacacccacc tgctgaagtc cctggaggca cagggcttca agagcgtggt 2280
cgctgtcatc gggctgccca acgacccgag cgtgcgcatg cacgaggcgc tcggatatgc 2340
cccccgcggc atgctgcggg cggccggctt caagcacggg aactggcatg acgtgggttt 2400
ctggcagctg gacttcagcc tgccggtacc gccccgtccg gtcctgcccg tcaccgagat 2460
ttgatgaccc gggggatcct agagtcctgc tttaatgaga tatgcgagac gcctatgatc 2520
gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct 2580
cagatcctta ccgccggttt cggttcattc taatgaatat atcacccgtt actatcgtat 2640
ttttatgaat aatattctcc gttcaattta ctgattgtac cctactactt atatgtacaa 2700
tattaaaatg aaaacaatat attgtgctga ataggtttat agcgacatct atgatagagc 2760
gccacaataa caaacaattg cgttttatta ttacaaatcc aattttaaaa aaagcggcag 2820
aaccggtcaa acctaaaaga ctgattacat aaatcttatt caaatttcaa aagtgcccca 2880
ggggctagta tctacgacac accgagcggc gaactaataa cgctcactga agggaactcc 2940
ggttccccgc cggcgcgcat gggtgagatt ccttgaagtt gagtattggc cgtccgctct 3000
accgaaagtt acgggcacca ttcaacccgg tccagcacgg cggccgggta accgacttgc 3060
tgccccgaga attatgcagc atttttttgg tgtatgtggg ccccaaatga agtgcaggtc 3120
aaaccttgac agtgacgaca aatcgttggg cgggtccagg gcgaattttg cgacaacatg 3180
tcgaggctca gcaggacctg caggcatgca agctctagag cggccgcttt aagagtgttg 3240
aggatgtgct tacttaaatt tcaaaagggc tatttaggga taatatttgg tgaagcatgg 3300
ctggtggact gaccataggt acccactgaa taaacatatc agcccaaagg cagagggtat 3360
ccctccctgt aatacgtact agtattcgag gagcgtgcta aagtagtatc ctctcttgat 3420
gctcctcgtc tccctccact gagtactccc ttcctctcct cattctcatc tcagtcctct 3480
cctcctccct cgaataagat agatccgccc ttgttgctga tctcatctcg cctcctattg 3540
gcctcgcccc tgccaactga ctgctctatt ggtgaccgtg gactttttgg gtatatatat 3600
ttcctgtcga tttgtttcca cttaaagtag caatgcttgg atctaaaact tattacttct 3660
cctgcttcag attctgatcc atttgtgttg attgaactca gggtgggatc gtggggctct 3720
gtatctatct aggatggata ttccaagcaa tttactctct tgagtgctgt tggtcttcct 3780
ctgctcctcg tctccgcttc ctctcagtct cctccattgc gctgcctgga acagatcgat 3840
ccgccttgtt gctgatctca tccatcctcc gtggagattt cgggtggcag tttccagtga 3900
ctattcagag caccatacta gtggcctggt aaaaatttcc ctcccatctt tcttgctatt 3960
ctcttcttat taatctgctg atttgcttga tggtaagttc ttacttattc ttccagatcg 4020
agtgaagcga catccgccgc cgtcttgtcc cctacgacgc cgctgtgttc tgctctgtcc 4080
tcctctgccg gtgaagcatc aacggtgacg gccatccggt gttgacgccg cggctgtgtt 4140
ctcctctgcc gtgaagtatc aaaggtgggt ttggatggat atcacagttt ggattggcaa 4200
ctctgttcta tagctcgttg aaattaaagc ttcacacagt tttgattgga ttacagctcc 4260
ccatattcat ctattttgca ccatgcatcg aatccactga cctccacacg ctcacggatc 4320
tggcctctgg tgcccacacg ctcacggatc cgacagagtg ctcccttcct atcatctcag 4380
tcctcatctc ctggtcgatc cggcttcttg tttggtgagc tcgtccaacc tccgagctcg 4440
cccctgacac ctgtggagtg gaggtatatt tctttccagc tgaagtacgc ttggatctaa 4500
aaacttacgt ctcctgcttc actgcttgct tgggagtctg atcgatttgt gttggttgaa 4560
tcatttgggg tggcagtgga gcagctagca agaatcatgg agtttccaat cattattcag 4620
agcaccacgc atggaatcca ctgacctggt aactttgtct catctttctt gctactgaaa 4680
tcttccacat ccccaccttc cttgttcatc tgctgatttc ccagattata cttccagatt 4740
gagtgaagcg ccgtcttgtc ccctcacgga tctggcctct ggtgcccagc cgccgctttc 4800
gcttcgttgt ccgacgaggc caccgccgct gtgttctgct ctgccgtgaa gcacaaaaag 4860
gtggggtctt cctctgctct ttctccactg acggcgatcc gaccttgaca cccgtggccg 4920
tggcagtggg gtcttcctct gctctgtttt tttaccagtt attggatcta aaaagggatc 4980
taaaagttat tacttctcct gcttgagatt ctgaccgatt tgtgttgatt gaatccaggg 5040
tggggtcgtg gggctctgta tctatctagt acggagtacc actgtattcc aagcaattta 5100
ctctcttgac tgctgttgga cttcctctgt ccccgcttcc attgtgctgc cttgaacaga 5160
tccatccacc ttgttgctaa tctcatccaa cctccgggta tgtttcttgt cgatttgttt 5220
ccagtaaagt agcaatgctt gggtctaaaa agtcattgct tctcctgctt gacgagtctc 5280
attgatttgc ccagtcatta ttcagagcac catgcatcca aggcgctgac ccggctataa 5340
gtttttatta cagggccgcc gtgttgtccc catcgagcaa agcgtttaat ccactgaccc 5400
gcttcgttgt ccgcacagcg tgaccatccg acgaagacgc cgccgttgtg ttctgctctc 5460
ctctgaagca tcaaaaggtg ggcttggtcc agatccccac attcatcgat ttggcttgcc 5520
aactcagttc caccagagct tggattgtgt tacagatccc taaattcatc gatttgcagg 5580
tttcagctga ttgatcaaga gagctctcaa tgatggcgga agttctggtg agcgcatcca 5640
caggggcaat gggttccctc ctgcggaagt tgggagctat gttgacggat gaatacaagc 5700
tgctcaagaa cgtccgtggg gacatcaagt tcctcaagga cgagctggag gtcatgtgcg 5760
cgttcctcct caagatgtca gatgtggagg agcctgacga accaacaaag ctgcgtgtga 5820
cggcggtgcg ggagatgtcc tataagatag aggacaacat cgacaagttc atggtcctcg 5880
tggaacacga gtcctcctgc tccgaggcgg ctcatggcgt cgccaagctc atggacaagt 5940
gcaagaactt gctgccggac atcaagaccc gccgcaggat cgccaaggag gtcaaagaca 6000
tcaagaaaga aattaaggat gtcagcgaca gattttcaag gtaccagatc ttgttctctt 6060
tacagtacta ttagtaccta ccatcagatt tgtgaccgat ttgttgccac tcaaggtaca 6120
agattgacga gtcctcctcc tctatgccgg caaaaggcaa ggtcgaccct cgacttcgtg 6180
cagtctacaa agacgccgca gagctcgtcg gaattgatgg accaaaggat gaacttgtca 6240
agtggctgaa tgagaaagag ggccaatcac tgaaatctgt ctctattgtt ggatatggag 6300
ggttgggcaa gaccacgctc gccaaccaga tccgggtcaa ccttggagcg accttcgact 6360
gcggggcttt tgtctcgatt tcacgcaagc ctgacatgaa ggcgatatta agatctatat 6420
tatcgcaaat caccaagaaa gacgaggctt gctctagatt agatgatata caactcatca 6480
tcgacaagat tcgagaattc ctccaagaca caaggtatgt atgtgagatc tactacgaag 6540
taacattgct taattagctc atgcataatc cgttgcctag aattttgcta atgagattgc 6600
tttgtgtgat ttactttcgt cctcaacttg tatgactgcg cttaaacttt caagtcattg 6660
tttcttgcaa cttatgtcga tctgttcact aattaagata tagctaaaat gtgtttgcca 6720
atttctgtcg ccacacattt tctaatttga ttgccacagg tttgttaaac ttgcggctat 6780
gttctgttta ttaaataatt gtgacatggc gtcagtcaaa acaataaagt acatgcaatt 6840
ccctctcttt tgtacaagat aaatatccgc acgcactaca tgactgcaaa tatatctatc 6900
ttctaaggac ccaaagcact tgcatgtgca gaccattgtt accggatatg cagtacatta 6960
ggtttgtgat tagccacttt aagaacatag ggtttctaga ggctataaac ctacggttat 7020
cagtattata atcttgaatg gatatcaaac atccgttgaa gaatctatgc ccataacaca 7080
aaaagagaaa agggaagaat taatcatagt cagatcggac cagcctgact agcgtaacat 7140
aatgacaaca gtaggttgtt gcgtctttcc tggtttcgta gcaatttgca tgacgaattg 7200
aggcgcagag ccaccactca atatgtggtc ctaccaatca tgaggaaata acttgttacc 7260
aaaatgacaa aagaaaatag gtagtcccca tcattgtttc aatatctatc ctcttgcgaa 7320
catgctattg gcagtggcaa aacattgtac cttgccactg gcaacagctg cctttactcg 7380
gcctgagcat tcatattttt ttcctcttgg ttgttaaatc ttgctatttt tcgcttgcct 7440
tctatggcaa tctttttttt tcttatattg ccaccaaatt gtatctaaga gcaactccaa 7500
cacgccgacg caatttttgt ccatttagat cggccaagcg gacggggtgt ctggttttgc 7560
ctttgggtgg tgcgcctacg cattagtccg cgtgggcatt taaaaaactt aaaaagctta 7620
aaataattaa atataaataa aaatgcatat taaagcccgt caaagtccac ataattaaac 7680
ataatacatt aatatattaa taaaaaacag ccgccccagc ccgcagccct aggcgtcttc 7740
gtcggaggag tgggtgaggt cgaagaatgc cggcagctca gcccacggga aggcctgctg 7800
gtgggcagcc agcaccgcct gctccgctgt ctgcggcggc ggcggcccta cctggcaccg 7860
cgcacgcctc ctcctcgcgc tcctgccgct cctcctcctc atcgcacgcc caacgaagtt 7920
ttggcgctcc tccccctcgg gttcccgctg catgcggacc tggccgtcgt accgctcctc 7980
ctccagcgtg cggtccttcc acgccacgag gtaggccgcc tcctcctcca tcacgcacag 8040
ccaaatgggc ggcttgctca tccgcttgca gacgatgccc tgccattggt aaacgccggt 8100
gggcgcgggt ggtgctagcg ctggtgcagg cggtgacgat gtggctggtg gtcccggcag 8160
gtagacacta tagctggcgg ccgatagctg cagcgtgtca tcgaggccag accactagga 8220
gtcctcgcac taagactcct cggtgacgtg atgggccccc tcctccaatg ccttgaggaa 8280
cgccgcctcc tcctcaacct actatttgac caccggtggc ggcggtggag ggcaggcttc 8340
gagctggacg gcgtcgcgga ggcgcacatg gtgctcgatg gtgaaccaga cttctcagtt 8400
aggggagtct ggcctcgatg ggtggagtcg cgacgctgct ctggcgtcag gagagcacgc 8460
cgggcccgaa tctcgttggc gcgcgcacgc tcggactgtg gcaccgccgg cactggaatg 8520
tggtgggggt cgaggtgcca gccgtggggg aagttcacgt ccggccacgg cagtgagacg 8580
cggtactccc ggtggtacta actacgcgtg gtgcaccggg atgtactggt ggtcccgtgg 8640
cgctgccgcc ctcccatccc gcagcgccga caccaatgcg aaccccttcg ctgccggcct 8700
caccggccct tgcaacgagc cggcctcgtg gtcgtgcttg ccttgccctt acctttgcca 8760
aagaaaccca tgacggctag ggttttctgt caccggcgag ggagcagatg tggactagag 8820
tgggaggaga agtggatgag gcccgccccg ccgcaccctt gcaattaaaa agggcgtgat 8880
ccggacgttt ctagtcactg ccatgcgggc ccgaggtagg tgggcgccat tgactacgtc 8940
tgtggtggta gttggctggc agacacgcca gcccgaggcg cacaccgagc ggacgcgcaa 9000
cacgtccgtc tcgtgttcgt gtgaacgcaa atcaggcaca aatttgggct ggaaatgcgt 9060
ctacatggat gccgaacgga cgtgatttgg gtttgtgtcc gcacgttggg ccggcgtttt 9120
tgtgcctttc gaccctaatg gacacacacg gaccaaatcc gttggcgtgt tagagttgct 9180
tgtacctagt actactatgt gtggagttgt tatctttgag tttctccccc atctgtagct 9240
gtgtattatt gtgagatgct ctcaggattt cacttatttt agataatgac aactttcttg 9300
atgctagaca attacatcaa ggtgtattat atctaggatg cacacagatg tgagattgtt 9360
tcaggatttt aggttacttg gtaggtgtta ctataaatat ctaatgagct ctaatgtgta 9420
tgttcatgaa actgtgttgc aggtacttta tcataattga tgatatatgg gagttaggaa 9480
catgggaaac tttgaaatgt gcatttgtca agaatacatt gggcagcaga ataattatca 9540
caacacgtat agttgatgtt gccaaatctt gttctccttc tagtgaagat cttgtctatg 9600
agatgaaacc actcagtgag gctgactcga agaaattgtt tttcaagagg atatttggct 9660
gtgaagaaag ctgtcctgat agcttgaaag aagctgccaa tgatattttg aaaaaatgcc 9720
gtggcctacc tctggccatc aatgccattt ctagcgtgtt ggtcacaaca agggaaacaa 9780
aagaagagtg ggatcgggtg cgacattcaa tacgctcttc aaaggtcaaa agtgatataa 9840
tagaaaccat gaattacata ttgtctctta gttattttga tcttccccac cacctaagaa 9900
gctgcttatt gtacctggct ctgtttcctg aagaccaatt gattggaagg aagcggttag 9960
tacgcagatg gatttctgaa ggctttatcc atggagaaag tggacaagat ctcatggaat 10020
taggagagga atattttcac cagcttgtga acagaagtct aatacagccc ggcaacatag 10080
ggtatgacgg caaggcaatg tattgccgag tccatgacac catccttgat ttcctaattg 10140
acaaatcctc tgaagagaac atgtgtactg tcctaaagaa acaatgcaag cctaatggca 10200
tagttcgtcg gctctctcta atggggaatg aggatgaaga aatcgtggag caattggatc 10260
tgtcacacgc tcggtcaatc actgcttttg gggatatcaa gctattgcct tcgcttggga 10320
ggtcaaaatg cttgcgcgtg ctagacttgc aagactgcga tcagttggaa aatcatcata 10380
tcaaggatat tgaaagactc tatcagctaa gatacttgga tatctcttct acaggaatca 10440
cggagcttcc caggcaaatt ggggagttgc tgtatctaga gacgctagtt gcctatggat 10500
tacgtgagct tcctgaaagc acaagtcggc tacaacgact ggcacgtttg tttgtttact 10560
ctggttgtaa gcttccaggt gggttgggaa acttgataaa cttgcaagag cttgactgcg 10620
ttgatgcctt gcatttgaag catgtggaag agctcggcaa actaacaaat ctgagaaagc 10680
tgagtattaa attggacact ggtgggattg aaggcaacaa attagaagag tccaaggaaa 10740
agctggtgtc ctctctctgt aaactggacg aatgcggcct cctttccctg agtattgatt 10800
attatctgag agaaaaggat ggagaagagc cgttcctccc tgctttgggt tgtatccagg 10860
aggtttttgt atatggacaa gatatcagcc ggattagcag atggctcgcg tcacttccca 10920
acctacacgg gttgcttttg gacaatccga agatagagca gcaggatatt gagatgattg 10980
gattgatacc caatctgatc gatttgacac tgtatttacg cataacagac gacgctgggc 11040
ggctcatcat caagcgtgaa ggatttcaac agttgcagag gtttgagctt tcccgtattc 11100
gtatggggga tttaatgttt gaaccggggg ctatgccgag gctcaaggag cttatactct 11160
accacttcat cgaaaagccc aaatctggtg cggtcgattt cgactttggc atccaacacc 11220
tctccagcct tgctcgcctc actgtcggct tattatgcgt tggctcgacg gcggcggaag 11280
tagaggctgc ggaggatgct ttcaagagca tggccgaggc gaaccccaac cgtccaatac 11340
tggagatgac aagactttat ccgcagcata tgttacaaga cgagcaaata ggtatgacag 11400
gctcaggtac tattaactag tttgtacttt tgtcttggtg agcagtagat caaaagatgt 11460
gcatatgctt caatcttttt tccggtaatc tatttcagct acaacgccgg ccgtcaagtc 11520
ctaggcttct gacgttgctt caggtgtgct ctcaatctat acatatttac tcatcatatt 11580
attctccacc tttttttcca tctctagagc tcagcttgtc attgcattgt tcaattgtgc 11640
ttctctagca gctacggaaa ttctgaacag gcaaaggtaa ttttacagaa gtttctttgg 11700
cataagtatc tgagaaaaat gcacttggca caatttacct tttcgcagta gaaaagagtc 11760
agtggatcat accttcttaa ttgttcgatt gctcgtaata cttgggggca ctctatgatt 11820
gctgtgtgct tttagttttg atatagttcc tgttagcatg gacagactgt gtgattggtt 11880
catgaatttc agggtggttc taatgaggaa atgatgcttg cttcagaaat agtttccatt 11940
tgtacctaag ctgtgtagtt ggtaaagttg cattttggat gttctgttgg tatagcttac 12000
agaagaaaga cttgcgttgt gcaggtacca ggggcgcagg ttactgctag tggcgtctga 12060
gatgtttcat aaacgttggg tgggcaccgg tcatgcaaaa aattgaagga taaaaaggtt 12120
ccgagaggtt gtgtgatgtg ctccttttaa atggcagtcg cttgcataaa gagatgttgc 12180
ttggtttttt agtaaagcgt tgtagtaagt tgctctgttt cggctgagag ccatcgttgt 12240
tgctgtctgt tacatcgaat ggctggctca tgtcatgttg atgttttttt tatctgaacc 12300
ggacaaacct gagggtgtag tatcatgttt gtaatgactg aaattctggg ctcacccgag 12360
ctccttaatt cgtaaacaac tgcgactcat tgttggattc cttacttcag aaaatattta 12420
cttgctctgg ctcatacagc tctgcatgtt ttgtagtggt ttgtcgctgt ttaggtgctg 12480
cttcagactc ctggtacctt tggccgctgc tgctactgca tgagctttgt atggcattgt 12540
tagtatagtt tggtgtcatt gggttttcgc cccatggtga gtgtccattg cgcgcactcg 12600
cagcggtttc ctggcgataa gttgaacttg atctttctct ttcccacctc ctaaacgtct 12660
attcagactt gtgtatattt gcattacggg gattacccga gttcaaaaat aaatattatt 12720
tactagttct gccaatgtgt ttccgttaca tacaaataaa agggtgcttc cagctacaag 12780
tatatttttg agaagggcca ttgcaaaacg aaatgaagcc caaattctac caacatgttt 12840
ctgtcacata tacattaaaa aaacggtgta aatgtcccgt caaaaaaggg tgtatgtagg 12900
aaaaggagtt cgacgccgcc agctccctcc taacgctcag attgtcttcg cggccgccac 12960
cgcggctgag tggctccttc aagcttgcgg atccgggcgg aaataggtaa agaagttgcg 13020
gataaggtaa ttgccattgc agattatttg gattgagagt gaatatgaga ctctaattgg 13080
ataccgaggg gaatttatgg aacgtcagtg gagcattttt gacaagaaat atttgctagc 13140
tgatagtgac cttaggcgac ttttgaacgc gcaataatgg tttctgacgt atgtgcttag 13200
ctcattaaac tccagaaacc cgcggctgag tggctccttc aacgttgcgg ttctgtcagt 13260
tccaaacgta aaacggcttg tcccgcgtca tcggcggggg tcataacgtg actcccttaa 13320
ttctccgctc atgatcagat tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac 13380
aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt 13440
aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt 13500
tcccctcggg agtgcttggc attccgtgcg ataatgactt ctgttcaacc acccaaacgt 13560
cggaaagcct gacgacggag cagcattcca aaaagatccc ttggctcgtc tgggtcggct 13620
agaaggtcga gtgggctgct gtggcttgat ccctcaacgc ggtcgcggac gtagcgcagc 13680
gccgaaaaat cctcgatcgc aaatccgacg ctgtcgaaaa gcgtgatctg cttgtcgctc 13740
tttcggccga cgtcctggcc agtcatcacg cgccaaagtt ccgtcacagg atgatctggc 13800
gcgagttgct ggatctcgcc ttcaatccgg gtctgtggcg ggaactccac gaaaatatcc 13860
gaacgcagca agatcgtcga cccctttccg acgctcaccg ggctggttgc cctcgccgct 13920
gggctggcgg ccgtctatgg ccctgcaaac gcgccagaaa cgccgtcgaa gccgtgtgcg 13980
agacaccgcg gccggccgcc ggcgttgtgg atacctcgcg gaaaacttgg ccctcactga 14040
cagatgaggg gcggacgttg acacttgagg ggccgactca cccggcgcgg cgttgacaga 14100
tgaggggcag gctcgatttc ggccggcgac gtggagctgg ccagcctcgc aaatcggcga 14160
aaacgcctga ttttacgcga gtttcccaca gatgatgtgg acaagcctgg ggataagtgc 14220
cctgcggtat tgacacttga ggggcgcgac tactgacaga tgaggggcgc gatccttgac 14280
acttgagggg cagagtgctg acagatgagg ggcgcaccta ttgacatttg aggggctgtc 14340
cacaggcaga aaatccagca tttgcaaggg tttccgcccg tttttcggcc accgctaacc 14400
tgtcttttaa cctgctttta aaccaatatt tataaacctt gtttttaacc agggctgcgc 14460
cctggcgcgt gaccgcgcac gccgaagggg ggtgcccccc cttctcgaac cctcccggcc 14520
cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc tcggccgcga acggcctcac 14580
cccaaaaatg gcagccaagc tcctaacatt ttattagaga gcaggctagt tgcttagata 14640
catgatcttc aggccgttat ctgtcagggc aagcgaaaat tggccattta tgacgaccaa 14700
tgccccgcag aagctcccat ctttgccgcc atagacgccg cgcccccctt ttggggtgta 14760
gaacatcctt ttgccagatg tggaaaagaa gttcgttgtc ccattgttgg caatgacgta 14820
gtagccggcg aaagtgcgag acccatttgc gctatatata agcctacgat ttccgttgcg 14880
actattgtcg taattggatg aactattatc gtagttgctc tcagagttgt cgtaatttga 14940
tggactattg tcgtaattgc ttatggagtt gtcgtagttg cttggagaaa tgtcgtagtt 15000
ggatggggag tagtcatagg gaagacgagc ttcatccact aaaacaattg gcaggtcagc 15060
aagtgcctgc cccgatgcca tcgcaagtac gaggcttaga accaccttca acagatcgcg 15120
catagtcttc cccagctctc taacgcttga gttaagccgc gccgcgaagc ggcgtcggct 15180
tgaacgaatt gttagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg 15240
aacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttgtc caagataagc 15300
ctgcctagct tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc 15360
ggcagcgaca tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa 15420
cgtaagcact acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa 15480
ggtttcattt agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc 15540
cgctggacct accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc 15600
aatgtcgatc gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc 15660
aaattgcagt tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat 15720
ggtgacttct acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag 15780
gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa 15840
atcaatatca ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc 15900
cagcaacgtc ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga 15960
tacttcggcg atcaccgctt ccctcatgat gtttaactcc tgaattaagc cgcgccgcga 16020
agcggtgtcg gcttgaatga attgttaggc gtcatcctgt gctcccgaga accagtacca 16080
gtacatcgct gtttcgttcg agacttgagg tctagtttta tacgtgaaca ggtcaatgcc 16140
gccgagagta aagccacatt ttgcgtacaa attgcaggca ggtacattgt tcgtttgtgt 16200
ctctaatcgt atgccaagga gctgtctgct tagtgcccac tttttcgcaa attcgatgag 16260
actgtgcgcg actcctttgc ctcggtgcgt gtgcgacaca acaatgtgtt cgatagaggc 16320
tagatcgttc catgttgagt tgagttcaat cttcccgaca agctcttggt cgatgaatgc 16380
gccatagcaa gcagagtctt catcagagtc atcatccgag atgtaatcct tccggtaggg 16440
gctcacactt ctggtagata gttcaaagcc ttggtcggat aggtgcacat cgaacacttc 16500
acgaacaatg aaatggttct cagcatccaa tgtttccgcc acctgctcag ggatcaccga 16560
aatcttcata tgacgcctaa cgcctggcac agcggatcgc aaacctggcg cggcttttgg 16620
cacaaaaggc gtgacaggtt tgcgaatccg ttgctgccac ttgtttaata gactggatgg 16680
aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg 16740
ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag 16800
atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg 16860
aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 16920
accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 16980
tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 17040
tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgatat cctttttttc 17100
tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 17160
cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 17220
caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 17280
cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 17340
cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 17400
gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 17460
acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 17520
atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 17580
cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 17640
gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 17700
tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 17760
tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 17820
agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta 17880
cgcatctgtg cggtatttca caccgcatat gaagatcggc ggcaatagct tcttagcgcc 17940
atcccggctg agaaagccca gtaaggaaac aactgtaggt tcgagtcgcg agatcccccg 18000
gaaccaaagg aagtaggtta aacccgctcc gatcaggccg agccacgcca ggccgagaac 18060
attggttcct gtaggcatcg ggattggcgg atcaaacact aaagctactg gaacgagcag 18120
aagtcctccg gccgccagtt gccaggcggt aaaggtgagc agaggcacgg gaggttgcca 18180
cttgcgggtc agcacggttc cgaacgccat ggaaaccgcc cccgccaggc ccgctgcgac 18240
gccgacagga tctagcgctg cgtttggtgt caacaccaac agcgccacgc ccgcagttcc 18300
gcaaatagcc cccaggaccg ccatcaatcg tatcgggcta cctagcagag cggcagagat 18360
gaacacgacc atcagcggct gcacagcgcc taccgtcgcc gcgaccccgc ccggcaggcg 18420
gtagaccgaa ataaacaaca agctccagaa tagcgaaata ttaagtgcgc cgaggatgaa 18480
gatgcgcatc caccagattc ccgttggaat ctgtcggacg atcatcacga gcaataaacc 18540
cgccggcaac gcccgcagca gcataccggc gacccctcgg cctcgctgtt cgggctccac 18600
gaaaacgccg gacagatgcg ccttgtgagc gtccttgggg ccgtcctcct gtttgaagac 18660
cgacagccca atgatctcgc cgtcgatgta ggcgccgaat gccacggcat ctcgcaaccg 18720
ttcagcgaac gcctccatgg gctttttctc ctcgtgctcg taaacggacc cgaacatctc 18780
tggagctttc ttcagggccg acaatcggat ctcgcggaaa tcctgcacgt cggccgctcc 18840
aagccgtcga atctgagcct taatcacaat tgtcaatttt aatcctctgt ttatcggcag 18900
ttcgtagagc gcgccgtgcg tcccgagcga tactgagcga agcaagtgcg tcgagcagtg 18960
cccgcttgtt cctgaaatgc cagtaaagcg ctggctgctg aacccccagc cggaactgac 19020
cccacaaggc cctagcgttt gcaatgcacc aggtcatcat tgacccaggc gtgttccacc 19080
aggccgctgc ctcgcaactc ttcgcaggct tcgccgacct gctcgcgcca cttcttcacg 19140
cgggtggaat ccgatccgca catgaggcgg aaggtttcca gcttgagcgg gtacggctcc 19200
cggtgcgagc tgaaatagtc gaacatccgt cgggccgtcg gcgacagctt gcggtacttc 19260
tcccatatga atttcgtgta gtggtcgcca gcaaacagca cgacgatttc ctcgtcgatc 19320
aggacctggc aacgggacgt tttcttgcca cggtccagga cgcggaagcg gtgcagcagc 19380
gacaccgatt ccaggtgccc aacgcggtcg gacgtgaagc ccatcgccgt cgcctgtagg 19440
cgcgacaggc attcctcggc cttcgtgtaa taccggccat tgatcgacca gcccaggtcc 19500
tggcaaagct cgtagaacgt gaaggtgatc ggctcgccga taggggtgcg cttcgcgtac 19560
tccaacacct gctgccacac cagttcgtca tcgtcggccc gcagctcgac gccggtgtag 19620
gtgatcttca cgtccttgtt gacgtggaaa atgaccttgt tttgcagcgc ctcgcgcggg 19680
attttcttgt tgcgcgtggt gaacagggca gagcgggccg tgtcgtttgg catcgctcgc 19740
atcgtgtccg gccacggcgc aatatcgaac aaggaaagct gcatttcctt gatctgctgc 19800
ttcgtgtgtt tcagcaacgc ggcctgcttg gcctcgctga cctgttttgc caggtcctcg 19860
ccggcggttt ttcgcttctt ggtcgtcata gttcctcgcg tgtcgatggt catcgacttc 19920
gccaaacctg ccgcctcctg ttcgagacga cgcgaacgct ccacggcggc cgatggcgcg 19980
ggcagggcag ggggagccag ttgcacgctg tcgcgctcga tcttggccgt agcttgctgg 20040
accatcgagc cgacggactg gaaggtttcg cggggcgcac gcatgacggt gcggcttgcg 20100
atggtttcgg catcctcggc ggaaaacccc gcgtcgatca gttcttgcct gtatgccttc 20160
cggtcaaacg tccgattcat tcaccctcct tgcgggattg ccccgactca cgccggggca 20220
atgtgccctt attcctgatt tgacccgcct ggtgccttgg tgtccagata atccacctta 20280
tcggcaatga agtcggtccc gtagaccgtc tggccgtcct tctcgtactt ggtattccga 20340
atcttgccct gcacgaatac cagctccgcg aagtcgctct tcttgatgga gcgcatgggg 20400
acgtgcttgg caatcacgcg caccccccgg ccgttttagc ggctaaaaaa gtcatggctc 20460
tgccctcggg cggaccacgc ccatcatgac cttgccaagc tcgtcctgct tctcttcgat 20520
cttcgccagc agggcgagga tcgtggcatc accgaaccgc gccgtgcgcg ggtcgtcggt 20580
gagccagagt ttcagcaggc cgcccaggcg gcccaggtcg ccattgatgc gggccagctc 20640
gcggacgtgc tcatagtcca cgacgcccgt gattttgtag ccctggccga cggccagcag 20700
gtaggccgac aggctcatgc cggccgccgc cgccttttcc tcaatcgctc ttcgttcgtc 20760
tggaaggcag tacaccttga taggtgggct gcccttcctg gttgggtaat gactccaact 20820
tattgatagt gttttatgtt cagataatgc ccgatgactt tgtcatgcag ctccaccgat 20880
tttgagaacg acagcgactt ccgtcccagc cgtgccaggt gctgcctcag attcaggtta 20940
tgccgctcaa ttcgctgcgt atatcgcttg ctgattacgt gcagctttcc cttcaggcgg 21000
gattcataca gcggccagcc atccgtcatc catatcacca cgtcaaaggg tgacagcagg 21060
ctcataagac gccccagcgt cgccatagtg cgttcaccga atacgtgcgc aacaaccgtc 21120
ttccggagac tgtcatacgc gtaaaacagc cagcgctggc gcgatttagc cccgacatag 21180
ccccactgtt cgtccatttc cgcgcagacg atgacgtcac tgcccggctg tatgcgcgag 21240
gttaccgact gcggcctgag ttttttaagt gacgtaaaat cgtgttgagg ccaacgccca 21300
taatgcgggc tgttgcccgg catccaacgc cattcatggc catatcaatg attttctggt 21360
gcgtaccggg ttgagaagcg gtgtaagtga actgcagttg ccatgtttta cggcagtgag 21420
agcagagata gcgctgatgt ccggcggtgc ttttgccgtt acgcaccacc ccgtcagtag 21480
ctgaacagga gggacagctg atagaaacag aagccactgg agcacctcaa aaacaccatc 21540
atacactaaa tcagtaagtt ggcagcatca cccctggttg gcttggtttc atcagccatc 21600
cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc aggattcccg 21660
ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct cgcgggtggg 21720
cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa gtctacacga 21780
accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga aaaaatcgct 21840
ataatgaccc cgaagcaggg ttatgcagcg gaaaagatcc 21880
<210> 156
<211> 21633
<212> DNA
<213> 人工序列
<220>
<223> 转化构建体
<400> 156
gtcgacgatc gtcaacgttc acttctaaag aaatagcgcc actcagcttc ctcagcggct 60
ttatccagcg atttcctatt atgtcggcat agttctcaag atcgacagcc tgtcacggtt 120
aagcgagaaa tgaataagaa ggctgataat tcggatctct gcgagggaga tgatatttga 180
tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc 240
cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca 300
aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg 360
tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca 420
ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt 480
ttttaatgta ctgaattcca atccccaaaa atctgagctt aacagcacag ttgctcctct 540
cagagcagaa tcgggtattc aacaccctca tatcaactac tacgttgtgt ataacggtcc 600
acatgccggt atatacgatg actggggttg tacaaaggcg gcaacaaacg gcgttcccgg 660
agttgcacac aagaaatttg ccactattac agaggcaaga gcagcagctg acgcgtacac 720
aacaagtcag caaacagaca ggttgaactt catccccaaa ggagaagctc aactcaagcc 780
caagagcttt gctaaggccc taacaagccc accaaagcaa aaagcccact ggctcacgct 840
aggaaccaaa aggcccagca gtgatccagc cccaaaagag atctcctttg ccccggagat 900
tacaatggac gatttcctct atctttacga tctaggaagg aagttcgaag gtgaaggtga 960
cgacactatg ttcaccactg ataatgagaa ggttagcctc ttcaatttca gaaagaatgc 1020
tgacccacag atggttagag aggcctacgc agcaggtctc atcaagacga tctacccgag 1080
taacaatctc caggagatca aataccttcc caagaaggtt aaagatgcag tcaaaagatt 1140
caggactaat tgcatcaaga acacagagaa agacatattt ctcaagatca gaagtactat 1200
tccagtatgg acgattcaag gcttgcttca taaaccaagg caagtaatag agattggagt 1260
ctctaaaaag gtagttccta ctgaatctaa ggccatgcat ggagtctaag attcaaatcg 1320
aggatctaac agaactcgcc gtgaagactg gcgaacagtt catacagagt cttttacgac 1380
tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca cgacactctg gtctactcca 1440
aaaatgtcaa agatacagtc tcagaagacc aaagggctat tgagactttt caacaaagga 1500
taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc atcgaaagga 1560
cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggctatca 1620
ttcaagatct ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg 1680
tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gacatctcca 1740
ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc tctatataag 1800
gaagttcatt tcatttggag aggacacgct cgagtataag agctctattt ttacaacaat 1860
taccaacaac aacaaacaac aaacaacatt acaattacta tttacaatta ccatgagccc 1920
agaacgacgc ccggccgaca tccgccgtgc caccgaggcg gacatgccgg cggtctgcac 1980
catcgtcaac cactacatcg agacaagcac ggtcaacttc cgtaccgagc cgcaggaacc 2040
gcaggagtgg acggacgacc tcgtccgtct gcgggagcgc tatccctggc tcgtcgccga 2100
ggtggacggc gaggtcgccg gcatcgccta cgcgggcccc tggaaggcac gcaacgccta 2160
cgactggacg gccgagtcaa ccgtgtacgt ctccccccgc caccagcgga cgggactggg 2220
ctccacgctc tacacccacc tgctgaagtc cctggaggca cagggcttca agagcgtggt 2280
cgctgtcatc gggctgccca acgacccgag cgtgcgcatg cacgaggcgc tcggatatgc 2340
cccccgcggc atgctgcggg cggccggctt caagcacggg aactggcatg acgtgggttt 2400
ctggcagctg gacttcagcc tgccggtacc gccccgtccg gtcctgcccg tcaccgagat 2460
ttgatgaccc gggggatcct agagtcctgc tttaatgaga tatgcgagac gcctatgatc 2520
gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct 2580
cagatcctta ccgccggttt cggttcattc taatgaatat atcacccgtt actatcgtat 2640
ttttatgaat aatattctcc gttcaattta ctgattgtac cctactactt atatgtacaa 2700
tattaaaatg aaaacaatat attgtgctga ataggtttat agcgacatct atgatagagc 2760
gccacaataa caaacaattg cgttttatta ttacaaatcc aattttaaaa aaagcggcag 2820
aaccggtcaa acctaaaaga ctgattacat aaatcttatt caaatttcaa aagtgcccca 2880
ggggctagta tctacgacac accgagcggc gaactaataa cgctcactga agggaactcc 2940
ggttccccgc cggcgcgcat gggtgagatt ccttgaagtt gagtattggc cgtccgctct 3000
accgaaagtt acgggcacca ttcaacccgg tccagcacgg cggccgggta accgacttgc 3060
tgccccgaga attatgcagc atttttttgg tgtatgtggg ccccaaatga agtgcaggtc 3120
aaaccttgac agtgacgaca aatcgttggg cgggtccagg gcgaattttg cgacaacatg 3180
tcgaggctca gcaggacctg caggcatgca agctctagag cggccgcgtg cagcgtgacc 3240
cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa aaaattacca 3300
catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata catatattta 3360
aactttactc tacgaataat ataatctata gtactacaat aatatcagtg ttttagagaa 3420
tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg acaacaggac 3480
tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa atagcttcac 3540
ctatataata cttcatccat tttattagta catccattta gggtttaggg ttaatggttt 3600
ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta aattaagaaa 3660
actaaaactc tattttagtt tttttattta ataatttaga tataaaatag aataaaataa 3720
agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg aaacattttt 3780
cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta acggacacca 3840
accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg catctctgtc 3900
gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc cgctgtcggc 3960
atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg gcctcctcct 4020
cctctcacgg cacggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc 4080
ctcgcccgcc gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttgt 4140
tcggagcgca cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt 4200
caaggtacgc cgctcgtcct cccccccccc ccctctctac cttctctaga tcggcgttcc 4260
ggtccatggt tagggcccgg tagttctact tctgttcatg tttgtgttag atccgtgttt 4320
gtgttagatc cgtgctgcta gcgttcgtac acggatgcga cctgtacgtc agacacgttc 4380
tgattgctaa cttgccagtg tttctctttg gggaatcctg ggatggctct agccgttccg 4440
cagacgggat cgatttcatg attttttttg tttcgttgca tagggtttgg tttgcccttt 4500
tcctttattt caatatatgc cgtgcacttg tttgtcgggt catcttttca tgcttttttt 4560
tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt ctagatcgga gtagaattct 4620
gtttcaaact acctggtgga tttattaatt ttggatctgt atgtgtgtgc catacatatt 4680
catagttacg aattgaagat gatggatgga aatatcgatc taggataggt atacatgttg 4740
atgcgggttt tactgatgca tatacagaga tgctttttgt tcgcttggtt gtgatgatgt 4800
ggtgtggttg ggcggtcgtt cattcgttct agatcggagt agaatactgt ttcaaactac 4860
ctggtgtatt tattaatttt ggaactgtat gtgtgtgtca tacatcttca tagttacgag 4920
tttaagatgg atggaaatat cgatctagga taggtataca tgttgatgtg ggttttactg 4980
atgcatatac atgatggcat atgcagcatc tattcatatg ctctaacctt gagtacctat 5040
ctattataat aaacaagtat gttttataat tattttgatc ttgatatact tggatgatgg 5100
catatgcagc agctatatgt ggattttttt agccctgcct tcatacgcta tttatttgct 5160
tggtactgtt tcttttgtcg atgctcaccc tgttgtttgg tgttacttct gcaggaatga 5220
tggcggaagt tctggtgagc gcatccacag gcgcaatggg ttccctcctc cggaagttgg 5280
gagctatgtt gacggatgaa tacaagctgc tcaagaacgt ccgtggggac atcaagttcc 5340
tcaaggacga gctggaggtc atgtgcgcgt tcctcctcaa gatgtcagat gtggaggagc 5400
ctgacgaacc aacaaagctg cgtgtgacgg cggtgcggga gatgtcctat aagatagagg 5460
acaacatcga caagttcatg gtcctcgtgg aacacgagtc ctcctgctcc gaggcggctc 5520
atggcgtcgc caagctcatg gacaagtgca agaacttgct gccggacatc aagacccgcc 5580
gcaggatcgc caaggaggtc aaagacatca agaaagaaat taaggatgtc agcgacagat 5640
tttcaaggta ccagatcttg ttctctttac agtactatta gtacctacca tcagatttgt 5700
gaccgatttg ttgccactca aggtacaaga ttgacgagtc ctcctcctct atgccggcaa 5760
aaggcaaggt cgaccctcga cttcgtgcag tctacaaaga cgccgcagag ctcgtcggaa 5820
ttgatggacc aaaggatgaa cttgtcaagt ggctgaatga gaaagagggc caatcactga 5880
aatctgtctc tattgttgga tatggagggt tgggcaagac cacgctcgcc aaccagatcc 5940
gggtcaacct tggagcgacc ttcgactgcg gggcttttgt ctcgatttca cgcaagcctg 6000
acatgaaggc gatattaaga tctatattat cacaaatcac caagaaagac gatgcttgct 6060
ctagattaga tgatatacaa ctcatcatcg acaagattcg agaattcctc caagacacaa 6120
ggtatgtatg tgagatctgc tacgaagtaa cattgcttaa ttagctcatg cataatcggt 6180
tgcctagaat tttgctaatg aagattgctt tgtgtgattt actttcgtcc tcaacttgta 6240
tgactgcgct taaactttca agtcattgtt tcttgcaact tatgtcgatc tgttcactaa 6300
ttaagatata gctaaaatgt gtttgccgat ttctgtcgcc acacattttc taatttgatt 6360
gccacaagtt tgttaaactt gcggctatgt tctgtttatt aaataattgt gacatggcgt 6420
cagtcaaaac aataaagtac atgcaattcc ctctcttttg tacaagataa atatccgcac 6480
gcactacatg actgcaaata tatctatctt ctaaggaccc aaagcacttg catgtgcaga 6540
ccattgttac cggatatgca gtacattagg tttgtgatta gccactttaa gaacataggg 6600
tttctagagg ctataaacct acggttatca gtattataat cttgaatgga tatcaaacat 6660
ccgttgaaga atctatgccc ataacacaaa aagagaaaag ggaagaatta atcatagtca 6720
gatcggacca gcctgactag cgtaacataa tgacaacagt aggttgttgc gtctttcctg 6780
gtttcgtagc aatttgcatg acgaattgag gcgcagagcc accactcaat atgtggtcct 6840
accaatcatg aggaaataac ttgttaccaa aatgacaaaa gaaaataggt agtccccatc 6900
attgtttcaa tatctatcct cttgcgaaca tgctattggc agtggcaaaa cattgtacct 6960
tgccactggc aacagctgcc tttactcggc ctgagcattc atattttttt cctcttggtt 7020
gttaaatctt gctatttttc gcttgccttc tatggcaatc tttttttttc ttatattgcc 7080
accaaattgt atctaagagc aactccaaca cgccgacgca atttttgtcc atttagatcg 7140
gccaagcgga cggggtgtct ggttttgcct ttgggtggtg cgcctacgca ttagtccgcg 7200
tgggcattta aaaaacttaa aaagcttaaa ataattaaat ataaataaaa atgcatatta 7260
aagcccatca aagtccacat aattaaacat aatacattaa tatattaata aaaaacagcc 7320
gccccagccc gcagccctag gcgtcttcgt cggaggagtg ggtgaggtcg aagaatgccg 7380
gcagctcagc ccacgggaag gcctgctggt gggcagccag caccgcctgc tccgctgtct 7440
gcggcggcgg cggccctacc tggcaccgcg cacgcctcct cctcgcgctc ctgccgctcc 7500
tcctcctcat cgcacgccca acgaagtttt ggcgctcctc cccctcgggt tcccgctgca 7560
tgcggacctg gccgtcgtac cgctcctcct ccagcgtgcg gtccttccac gccacgaggt 7620
aggccgcctc ctcctccatc acgcacagcc aaatgggcgg cttgctcatc cgcttgcaga 7680
cgatgccctg ccattggtaa acgccggtgg gcgcgggtgg tgctagcgct ggtgcaggcg 7740
gtgacgatgt ggctggtggt cccggcaggt agacactata gttggcggcc gatagctgca 7800
gcgtgtcatc gaggccagac cactaggagt cctcgcacta agactcctcg gtgacgtgat 7860
gggccccctc ctccaatgcc ttgaggaacg ccgcctcctc ctcaacctac tatttgacca 7920
ccggtggcgg cggtggaggg caggcttcga gctggacggc gtcgcggagg cgcacatggt 7980
gctcgatggt gaaccagact tctcagttag gggagtctgg cctcgatggg tggagtcgcg 8040
acgctgctct ggcgtcagga gagcacgccg ggcccgaatc tcgttggcgc gcgcacgctc 8100
ggactgtggc accgccggca ctggaatgtg gtgggggtcg aggtgccagc cgtgggggaa 8160
gttcacgtcc ggccatggca gtgagacgcg gtactcccgg tggtactaac tacgcgtggt 8220
gcaccgggat gtactggtgg tcccgtggcg ctgccgccct cccatcccgc agcgccgaca 8280
ccaatgcgaa ccccttcgct gccggcctca ccggcccttg caacgagccg gcctcgtggt 8340
cgtgcttgcc ttgcccttac ctttgccaaa gaaacccatg acggctaggg ttttctgtca 8400
ccggcgaggg agcagatgtg gactagagtg ggaggagaag tggatgaggc ccgccccgcc 8460
gcacccttgc aattaaaaag ggcgtgatcc ggacgtttct agtcactgcc atgcgggccc 8520
gaggtaggtg ggcggcattg actacgtctg tggtggtagt tggctggcag acacgccagc 8580
ccgaggcgca caccgagcgg acgcgcaaca cgtccgtctc gtgttcgtgt gaacgcaaat 8640
caggcacaaa tttgggctgg aaatgcgtct acatggatgc cgaacggacg tgatttgggt 8700
ttgtgtccgc acgttgggcc ggcgtttttg tgcctttcga ccctaatgga cacacacgga 8760
ccaaatccgt tggcgtgtta gagttgcttg tacctagtac tactatgtgt ggagttgtta 8820
tctttgagtt tctcccccat ctgtagctgt gtattattgt gagatgctct caggatttca 8880
cttattttag ataatgacaa ctttcttgat gctagacaat tacatcaagg tgtattatat 8940
ctaggatgca cacagatgtg agattgtttc aggattttag gttacttggt aggtgttact 9000
ataaatatct aatgagctct aatgtgtatg ttcatgaaac tgtgttgcag gtactttatc 9060
ataattgatg atatatggga gttaggaaca tgggaaactt tgaaatgtgc atttgtcaag 9120
aatacattgg gcagcagaat aattatcaca acacgtatag ttgatgttgc caaatcttgt 9180
tctccttcta gtgaagatct tgtctatgag atgaaaccac tcagtgaggc tgactcgaag 9240
aaattgtttt tcaagaggat atttggctgt gaagaaagct gtcctgatag cttgaaagaa 9300
gctgccaatg atattttgaa aaaatgccgt ggcctacctc tggccatcaa tgccatttct 9360
agcttgttgg tcacaacaag ggaaacaaaa gaagagtggg atcgggtgcg acattcaata 9420
cgctcttcaa aggtcaaaag tgatataata gaaaccatga attacatatt gtctcttagt 9480
tattttgatc ttccccacca cctaagaagc tgcttattgt acctggctct gtttcctgaa 9540
gaccaattga ttggaaggaa gcggttagta cgcagatgga tttctgaagg ctttatccat 9600
ggagaaagtg gacaagatct catggaatta ggagaggaat attttcacca gcttgtgaac 9660
agaagtctaa tacagcccgg caacataggg tatgacggca aggcggagta ttgccgagtc 9720
catgacacca tccttgattt cctaattgac aaatcctctg aagagaacat gtgtactgtc 9780
ctgaagaaac aatgcaagcc taatggcata gttcgtcggc tctctctaat ggggaatgag 9840
gatgaagaaa tcatggagca attggatctg tcacacgctc ggtcaatcag tgcttttggg 9900
gatatcaagc tattgccttc gcttgggagg tcaaaatgct tgcgcgtgct agacttgcaa 9960
ggctgctatc agttgaaaaa tcatcatatc aaggatattg aaagactcta tcagctgaga 10020
tacttggata tctcttttac aggaatcacg gagcttccca ggcaaattgg ggagttgctg 10080
tatctagaga cgctagttag tacatcctat ggattacgtg agcttcctga aagcacaagt 10140
cggctacaac gactggcacg tttgtttgtt gacagcggtt gtaagcttcc agatgggttg 10200
ggaaacttga taaacttgca agagcttgac tgcgttgatg ccttgcagtt gaagcatgtg 10260
gaagagctcg gcaaactaac aaatctgaga aagctgagga ttaaattgga cactgatggg 10320
attgaaggca acaaattaga acagtccaag gaaaagctgg tgtcctctct ctgtaaactg 10380
gacgaatgcg gcctcctttc cctgagtatt tattattatc tgagagaaaa ggatggagaa 10440
gagccgttcc tccctgcttt gggttgtatc caggaggttt ttgtacatgg acaagatatc 10500
agccggatta gcagatggct cgcgtcactt cccaacctac acaggttgtt tttgaaccaa 10560
ccgaagatag agcagcagga tattgagatg attggattga tacccaatct gatggatttg 10620
acactgtatt tatgcataac agacgacgct gggcggctca tcatcaagcg tgaaggattt 10680
caacagttgc agaggtttga gctttcccgt attcgtatgg gggatttaat gtttgaaccg 10740
ggggctatgc cgaggctcaa ggagcttata ctctacgact tcatcggaaa gcccaaatct 10800
ggtgcggtcg atttcgactt tggcatccaa cacctctcca gccttgctcg cctcactgtc 10860
ggcttatcct gcgttggctc gacggtggcg gaagtagagg ccgcggagga tgctttcaag 10920
agcatggccg aggcgaaccc caaccgtcca atactggaga tgacaagata taatacgcag 10980
catatgttac aagacgagca aataggtatg acaggctcag gtactattaa ctagtttgta 11040
cttttgtctt ggtgagcggt agatcaaaag atgtgcatat gcttcaatct tttttccggt 11100
aatctatttc agctacaacg ccggccgtca agtcctaggc tttgcctaaa ccgagatgcg 11160
cctttggtta tggagcccaa gtcacgtact cggattggat gctgcagcaa atgatacttc 11220
tgtacatgtc tgcaagtatc acgaattagt ggaatgacta ggaataagta agacaagtat 11280
cacgaattca gagtcggatg caattttttt cttttctttt gggcataagt agattgtcat 11340
ttgagtattt ttttccatta atatttgtgt attcttagat tgagactttg atatgtaacc 11400
cagatttctg ttgttaggaa acacataccg tgtagcttat cagaagaaaa agatctagcg 11460
tgcatctata ttctatcgga ctatgatatt tggcacatgt gtgccatata aacaaaactg 11520
tcacacctct atttccttca ctctaaatag atgtatacat tagtttgatt ctttcttctt 11580
tcacgtgcat ttctccttct aattagatgc atacattaga ggacaaaaaa gacgatgtca 11640
ttctagattt tctttattaa aatatttaaa atgttttgta gctcaaaccg tcggtccgat 11700
gtaaaaaccg tttttacata tacgattcgt cgcgatgaga tctttgaaac tagatcctat 11760
gttgacatgt ttcgatgact ttttttaacc aaaatttacc atgcctttta tatgcaagtt 11820
accgtgctat ttccacataa gtcatcggag gcataaaaat ggttcaccga cactacagtt 11880
catatattac catgatgttt acatataagt taccgggagg gggggggggg gtgtctgttt 11940
tcaataattt ccccaccgag tcaaatatta ccatagtata tgtacgtaag ttatcaggta 12000
tccgatgggt atattaccat ggctatcttt taaaaaaatc tctaatttac cgcggtgttt 12060
gtatctaaat tatcaagttt gtggtgccta atttactatg ctatttacat acaaaagtta 12120
ccagtgtatt ttttgacaat tttttactat tcttccccaa atcaagacta ttaccgtgct 12180
gtttacacac aagttaccgg gtatattttc aacaacttca tccacccgaa tcaaacttat 12240
catgatgttt gtgagtaact tatcatgtct gcaatgcgta tattaccatt ctatttaaac 12300
aaaatttacc aacgatatgc ttcaaacaat tttttacgtg ggtcaaagct agcatactgt 12360
ttttgcgtaa gttattgtat ctgcaataaa tatattaccg tgctatttac ctagaagtta 12420
ttgggggtaa tgttcttcaa cgaaaaaaaa ttcagggtca aaaaattatt gtggtgtttg 12480
aacgagagtt attagctctc cgatgggtaa attatcatgt tatttgcaca taaattaccg 12540
agggtatgct tttcaacaac ttcctccatc caggtcaaag ttatcatggt gtttttgctc 12600
aagttttcaa gtttgtggtg cgtatattac cacgatgttg actgtttaca aggtttttga 12660
gtcgccgact aatcgccgac taatcgtcga gtcgtcgcgg cggcggccgc caccgcggct 12720
gagtggctcc ttcaagcttg cggatccggg cggaaatagg taaagaagtt gcggataagg 12780
taattgccat tgcagattat ttggattgag agtgaatatg agactctaat tggataccga 12840
ggggaattta tggaacgtca gtggagcatt tttgacaaga aatatttgct agctgatagt 12900
gaccttaggc gacttttgaa cgcgcaataa tggtttctga cgtatgtgct tagctcatta 12960
aactccagaa acccgcggct gagtggctcc ttcaacgttg cggttctgtc agttccaaac 13020
gtaaaacggc ttgtcccgcg tcatcggcgg gggtcataac gtgactccct taattctccg 13080
ctcatgatca gattgtcgtt tcccgccttc agtttaaact atcagtgttt gacaggatat 13140
attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg 13200
cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc 13260
gggagtgctt ggcattccgt gcgataatga cttctgttca accacccaaa cgtcggaaag 13320
cctgacgacg gagcagcatt ccaaaaagat cccttggctc gtctgggtcg gctagaaggt 13380
cgagtgggct gctgtggctt gatccctcaa cgcggtcgcg gacgtagcgc agcgccgaaa 13440
aatcctcgat cgcaaatccg acgctgtcga aaagcgtgat ctgcttgtcg ctctttcggc 13500
cgacgtcctg gccagtcatc acgcgccaaa gttccgtcac aggatgatct ggcgcgagtt 13560
gctggatctc gccttcaatc cgggtctgtg gcgggaactc cacgaaaata tccgaacgca 13620
gcaagatcgt cgaccccttt ccgacgctca ccgggctggt tgccctcgcc gctgggctgg 13680
cggccgtcta tggccctgca aacgcgccag aaacgccgtc gaagccgtgt gcgagacacc 13740
gcggccggcc gccggcgttg tggatacctc gcggaaaact tggccctcac tgacagatga 13800
ggggcggacg ttgacacttg aggggccgac tcacccggcg cggcgttgac agatgagggg 13860
caggctcgat ttcggccggc gacgtggagc tggccagcct cgcaaatcgg cgaaaacgcc 13920
tgattttacg cgagtttccc acagatgatg tggacaagcc tggggataag tgccctgcgg 13980
tattgacact tgaggggcgc gactactgac agatgagggg cgcgatcctt gacacttgag 14040
gggcagagtg ctgacagatg aggggcgcac ctattgacat ttgaggggct gtccacaggc 14100
agaaaatcca gcatttgcaa gggtttccgc ccgtttttcg gccaccgcta acctgtcttt 14160
taacctgctt ttaaaccaat atttataaac cttgttttta accagggctg cgccctggcg 14220
cgtgaccgcg cacgccgaag gggggtgccc ccccttctcg aaccctcccg gcccgctaac 14280
gcgggcctcc catcccccca ggggctgcgc ccctcggccg cgaacggcct caccccaaaa 14340
atggcagcca agctcctaac attttattag agagcaggct agttgcttag atacatgatc 14400
ttcaggccgt tatctgtcag ggcaagcgaa aattggccat ttatgacgac caatgccccg 14460
cagaagctcc catctttgcc gccatagacg ccgcgccccc cttttggggt gtagaacatc 14520
cttttgccag atgtggaaaa gaagttcgtt gtcccattgt tggcaatgac gtagtagccg 14580
gcgaaagtgc gagacccatt tgcgctatat ataagcctac gatttccgtt gcgactattg 14640
tcgtaattgg atgaactatt atcgtagttg ctctcagagt tgtcgtaatt tgatggacta 14700
ttgtcgtaat tgcttatgga gttgtcgtag ttgcttggag aaatgtcgta gttggatggg 14760
gagtagtcat agggaagacg agcttcatcc actaaaacaa ttggcaggtc agcaagtgcc 14820
tgccccgatg ccatcgcaag tacgaggctt agaaccacct tcaacagatc gcgcatagtc 14880
ttccccagct ctctaacgct tgagttaagc cgcgccgcga agcggcgtcg gcttgaacga 14940
attgttagac attatttgcc gactaccttg gtgatctcgc ctttcacgta gtgaacaaat 15000
tcttccaact gatctgcgcg cgaggccaag cgatcttctt gtccaagata agcctgccta 15060
gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca gtcggcagcg 15120
acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga caacgtaagc 15180
actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt taaggtttca 15240
tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc cgccgctgga 15300
cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag atcaatgtcg 15360
atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc tccaaattgc 15420
agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac aatggtgact 15480
tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa aaggtcgttg 15540
atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag caaatcaata 15600
tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac ggccagcaac 15660
gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt cgatacttcg 15720
gcgatcaccg cttccctcat gatgtttaac tcctgaatta agccgcgccg cgaagcggtg 15780
tcggcttgaa tgaattgtta ggcgtcatcc tgtgctcccg agaaccagta ccagtacatc 15840
gctgtttcgt tcgagacttg aggtctagtt ttatacgtga acaggtcaat gccgccgaga 15900
gtaaagccac attttgcgta caaattgcag gcaggtacat tgttcgtttg tgtctctaat 15960
cgtatgccaa ggagctgtct gcttagtgcc cactttttcg caaattcgat gagactgtgc 16020
gcgactcctt tgcctcggtg cgtgtgcgac acaacaatgt gttcgataga ggctagatcg 16080
ttccatgttg agttgagttc aatcttcccg acaagctctt ggtcgatgaa tgcgccatag 16140
caagcagagt cttcatcaga gtcatcatcc gagatgtaat ccttccggta ggggctcaca 16200
cttctggtag atagttcaaa gccttggtcg gataggtgca catcgaacac ttcacgaaca 16260
atgaaatggt tctcagcatc caatgtttcc gccacctgct cagggatcac cgaaatcttc 16320
atatgacgcc taacgcctgg cacagcggat cgcaaacctg gcgcggcttt tggcacaaaa 16380
ggcgtgacag gtttgcgaat ccgttgctgc cacttgttta atagactgga tggaggcgga 16440
taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa 16500
atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa 16560
gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa 16620
tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt 16680
ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt 16740
gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg 16800
agcgtcagac cccgtagaaa agatcaaagg atcttcttga tatccttttt ttctgcgcgt 16860
aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca 16920
agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac 16980
tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac 17040
atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct 17100
taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg 17160
gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca 17220
gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt 17280
aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta 17340
tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc 17400
gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc 17460
cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa 17520
ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag 17580
cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct 17640
gtgcggtatt tcacaccgca tatgaagatc ggcggcaata gcttcttagc gccatcccgg 17700
ctgagaaagc ccagtaagga aacaactgta ggttcgagtc gcgagatccc ccggaaccaa 17760
aggaagtagg ttaaacccgc tccgatcagg ccgagccacg ccaggccgag aacattggtt 17820
cctgtaggca tcgggattgg cggatcaaac actaaagcta ctggaacgag cagaagtcct 17880
ccggccgcca gttgccaggc ggtaaaggtg agcagaggca cgggaggttg ccacttgcgg 17940
gtcagcacgg ttccgaacgc catggaaacc gcccccgcca ggcccgctgc gacgccgaca 18000
ggatctagcg ctgcgtttgg tgtcaacacc aacagcgcca cgcccgcagt tccgcaaata 18060
gcccccagga ccgccatcaa tcgtatcggg ctacctagca gagcggcaga gatgaacacg 18120
accatcagcg gctgcacagc gcctaccgtc gccgcgaccc cgcccggcag gcggtagacc 18180
gaaataaaca acaagctcca gaatagcgaa atattaagtg cgccgaggat gaagatgcgc 18240
atccaccaga ttcccgttgg aatctgtcgg acgatcatca cgagcaataa acccgccggc 18300
aacgcccgca gcagcatacc ggcgacccct cggcctcgct gttcgggctc cacgaaaacg 18360
ccggacagat gcgccttgtg agcgtccttg gggccgtcct cctgtttgaa gaccgacagc 18420
ccaatgatct cgccgtcgat gtaggcgccg aatgccacgg catctcgcaa ccgttcagcg 18480
aacgcctcca tgggcttttt ctcctcgtgc tcgtaaacgg acccgaacat ctctggagct 18540
ttcttcaggg ccgacaatcg gatctcgcgg aaatcctgca cgtcggccgc tccaagccgt 18600
cgaatctgag ccttaatcac aattgtcaat tttaatcctc tgtttatcgg cagttcgtag 18660
agcgcgccgt gcgtcccgag cgatactgag cgaagcaagt gcgtcgagca gtgcccgctt 18720
gttcctgaaa tgccagtaaa gcgctggctg ctgaaccccc agccggaact gaccccacaa 18780
ggccctagcg tttgcaatgc accaggtcat cattgaccca ggcgtgttcc accaggccgc 18840
tgcctcgcaa ctcttcgcag gcttcgccga cctgctcgcg ccacttcttc acgcgggtgg 18900
aatccgatcc gcacatgagg cggaaggttt ccagcttgag cgggtacggc tcccggtgcg 18960
agctgaaata gtcgaacatc cgtcgggccg tcggcgacag cttgcggtac ttctcccata 19020
tgaatttcgt gtagtggtcg ccagcaaaca gcacgacgat ttcctcgtcg atcaggacct 19080
ggcaacggga cgttttcttg ccacggtcca ggacgcggaa gcggtgcagc agcgacaccg 19140
attccaggtg cccaacgcgg tcggacgtga agcccatcgc cgtcgcctgt aggcgcgaca 19200
ggcattcctc ggccttcgtg taataccggc cattgatcga ccagcccagg tcctggcaaa 19260
gctcgtagaa cgtgaaggtg atcggctcgc cgataggggt gcgcttcgcg tactccaaca 19320
cctgctgcca caccagttcg tcatcgtcgg cccgcagctc gacgccggtg taggtgatct 19380
tcacgtcctt gttgacgtgg aaaatgacct tgttttgcag cgcctcgcgc gggattttct 19440
tgttgcgcgt ggtgaacagg gcagagcggg ccgtgtcgtt tggcatcgct cgcatcgtgt 19500
ccggccacgg cgcaatatcg aacaaggaaa gctgcatttc cttgatctgc tgcttcgtgt 19560
gtttcagcaa cgcggcctgc ttggcctcgc tgacctgttt tgccaggtcc tcgccggcgg 19620
tttttcgctt cttggtcgtc atagttcctc gcgtgtcgat ggtcatcgac ttcgccaaac 19680
ctgccgcctc ctgttcgaga cgacgcgaac gctccacggc ggccgatggc gcgggcaggg 19740
cagggggagc cagttgcacg ctgtcgcgct cgatcttggc cgtagcttgc tggaccatcg 19800
agccgacgga ctggaaggtt tcgcggggcg cacgcatgac ggtgcggctt gcgatggttt 19860
cggcatcctc ggcggaaaac cccgcgtcga tcagttcttg cctgtatgcc ttccggtcaa 19920
acgtccgatt cattcaccct ccttgcggga ttgccccgac tcacgccggg gcaatgtgcc 19980
cttattcctg atttgacccg cctggtgcct tggtgtccag ataatccacc ttatcggcaa 20040
tgaagtcggt cccgtagacc gtctggccgt ccttctcgta cttggtattc cgaatcttgc 20100
cctgcacgaa taccagctcc gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct 20160
tggcaatcac gcgcaccccc cggccgtttt agcggctaaa aaagtcatgg ctctgccctc 20220
gggcggacca cgcccatcat gaccttgcca agctcgtcct gcttctcttc gatcttcgcc 20280
agcagggcga ggatcgtggc atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag 20340
agtttcagca ggccgcccag gcggcccagg tcgccattga tgcgggccag ctcgcggacg 20400
tgctcatagt ccacgacgcc cgtgattttg tagccctggc cgacggccag caggtaggcc 20460
gacaggctca tgccggccgc cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg 20520
cagtacacct tgataggtgg gctgcccttc ctggttgggt aatgactcca acttattgat 20580
agtgttttat gttcagataa tgcccgatga ctttgtcatg cagctccacc gattttgaga 20640
acgacagcga cttccgtccc agccgtgcca ggtgctgcct cagattcagg ttatgccgct 20700
caattcgctg cgtatatcgc ttgctgatta cgtgcagctt tcccttcagg cgggattcat 20760
acagcggcca gccatccgtc atccatatca ccacgtcaaa gggtgacagc aggctcataa 20820
gacgccccag cgtcgccata gtgcgttcac cgaatacgtg cgcaacaacc gtcttccgga 20880
gactgtcata cgcgtaaaac agccagcgct ggcgcgattt agccccgaca tagccccact 20940
gttcgtccat ttccgcgcag acgatgacgt cactgcccgg ctgtatgcgc gaggttaccg 21000
actgcggcct gagtttttta agtgacgtaa aatcgtgttg aggccaacgc ccataatgcg 21060
ggctgttgcc cggcatccaa cgccattcat ggccatatca atgattttct ggtgcgtacc 21120
gggttgagaa gcggtgtaag tgaactgcag ttgccatgtt ttacggcagt gagagcagag 21180
atagcgctga tgtccggcgg tgcttttgcc gttacgcacc accccgtcag tagctgaaca 21240
ggagggacag ctgatagaaa cagaagccac tggagcacct caaaaacacc atcatacact 21300
aaatcagtaa gttggcagca tcacccctgg ttggcttggt ttcatcagcc atccgcttgc 21360
cctcatctgt tacgccggcg gtagccggcc agcctcgcag agcaggattc ccgttgagca 21420
ccgccaggtg cgaataaggg acagtgaaga aggaacaccc gctcgcgggt gggcctactt 21480
cacctatcct gcccggctga cgccgttgga tacaccaagg aaagtctaca cgaacccttt 21540
ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac cgaaaaaatc gctataatga 21600
ccccgaagca gggttatgca gcggaaaaga tcc 21633
<210> 157
<211> 21878
<212> DNA
<213> 人工序列
<220>
<223> 转化构建体
<400> 157
gtcgacgatc gtcaacgttc acttctaaag aaatagcgcc actcagcttc ctcagcggct 60
ttatccagcg atttcctatt atgtcggcat agttctcaag atcgacagcc tgtcacggtt 120
aagcgagaaa tgaataagaa ggctgataat tcggatctct gcgagggaga tgatatttga 180
tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc 240
cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca 300
aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg 360
tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca 420
ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt 480
ttttaatgta ctgaattcca atccccaaaa atctgagctt aacagcacag ttgctcctct 540
cagagcagaa tcgggtattc aacaccctca tatcaactac tacgttgtgt ataacggtcc 600
acatgccggt atatacgatg actggggttg tacaaaggcg gcaacaaacg gcgttcccgg 660
agttgcacac aagaaatttg ccactattac agaggcaaga gcagcagctg acgcgtacac 720
aacaagtcag caaacagaca ggttgaactt catccccaaa ggagaagctc aactcaagcc 780
caagagcttt gctaaggccc taacaagccc accaaagcaa aaagcccact ggctcacgct 840
aggaaccaaa aggcccagca gtgatccagc cccaaaagag atctcctttg ccccggagat 900
tacaatggac gatttcctct atctttacga tctaggaagg aagttcgaag gtgaaggtga 960
cgacactatg ttcaccactg ataatgagaa ggttagcctc ttcaatttca gaaagaatgc 1020
tgacccacag atggttagag aggcctacgc agcaggtctc atcaagacga tctacccgag 1080
taacaatctc caggagatca aataccttcc caagaaggtt aaagatgcag tcaaaagatt 1140
caggactaat tgcatcaaga acacagagaa agacatattt ctcaagatca gaagtactat 1200
tccagtatgg acgattcaag gcttgcttca taaaccaagg caagtaatag agattggagt 1260
ctctaaaaag gtagttccta ctgaatctaa ggccatgcat ggagtctaag attcaaatcg 1320
aggatctaac agaactcgcc gtgaagactg gcgaacagtt catacagagt cttttacgac 1380
tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca cgacactctg gtctactcca 1440
aaaatgtcaa agatacagtc tcagaagacc aaagggctat tgagactttt caacaaagga 1500
taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc atcgaaagga 1560
cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggctatca 1620
ttcaagatct ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg 1680
tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gacatctcca 1740
ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc tctatataag 1800
gaagttcatt tcatttggag aggacacgct cgagtataag agctctattt ttacaacaat 1860
taccaacaac aacaaacaac aaacaacatt acaattacta tttacaatta ccatgagccc 1920
agaacgacgc ccggccgaca tccgccgtgc caccgaggcg gacatgccgg cggtctgcac 1980
catcgtcaac cactacatcg agacaagcac ggtcaacttc cgtaccgagc cgcaggaacc 2040
gcaggagtgg acggacgacc tcgtccgtct gcgggagcgc tatccctggc tcgtcgccga 2100
ggtggacggc gaggtcgccg gcatcgccta cgcgggcccc tggaaggcac gcaacgccta 2160
cgactggacg gccgagtcaa ccgtgtacgt ctccccccgc caccagcgga cgggactggg 2220
ctccacgctc tacacccacc tgctgaagtc cctggaggca cagggcttca agagcgtggt 2280
cgctgtcatc gggctgccca acgacccgag cgtgcgcatg cacgaggcgc tcggatatgc 2340
cccccgcggc atgctgcggg cggccggctt caagcacggg aactggcatg acgtgggttt 2400
ctggcagctg gacttcagcc tgccggtacc gccccgtccg gtcctgcccg tcaccgagat 2460
ttgatgaccc gggggatcct agagtcctgc tttaatgaga tatgcgagac gcctatgatc 2520
gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct 2580
cagatcctta ccgccggttt cggttcattc taatgaatat atcacccgtt actatcgtat 2640
ttttatgaat aatattctcc gttcaattta ctgattgtac cctactactt atatgtacaa 2700
tattaaaatg aaaacaatat attgtgctga ataggtttat agcgacatct atgatagagc 2760
gccacaataa caaacaattg cgttttatta ttacaaatcc aattttaaaa aaagcggcag 2820
aaccggtcaa acctaaaaga ctgattacat aaatcttatt caaatttcaa aagtgcccca 2880
ggggctagta tctacgacac accgagcggc gaactaataa cgctcactga agggaactcc 2940
ggttccccgc cggcgcgcat gggtgagatt ccttgaagtt gagtattggc cgtccgctct 3000
accgaaagtt acgggcacca ttcaacccgg tccagcacgg cggccgggta accgacttgc 3060
tgccccgaga attatgcagc atttttttgg tgtatgtggg ccccaaatga agtgcaggtc 3120
aaaccttgac agtgacgaca aatcgttggg cgggtccagg gcgaattttg cgacaacatg 3180
tcgaggctca gcaggacctg caggcatgca agctctagag cggccgcttt aagagtgttg 3240
aggatgtgct tacttaaatt tcaaaagggc tatttaggga taatatttgg tgaagcatgg 3300
ctggtggact gaccataggt acccactgaa taaacatatc agcccaaagg cagagggtat 3360
ccctccctgt aatacgtact agtattcgag gagcgtgcta aagtagtatc ctctcttgat 3420
gctcctcgtc tccctccact gagtactccc ttcctctcct cattctcatc tcagtcctct 3480
cctcctccct cgaataagat agatccgccc ttgttgctga tctcatctcg cctcctattg 3540
gcctcgcccc tgccaactga ctgctctatt ggtgaccgtg gactttttgg gtatatatat 3600
ttcctgtcga tttgtttcca cttaaagtag caatgcttgg atctaaaact tattacttct 3660
cctgcttcag attctgatcc atttgtgttg attgaactca gggtgggatc gtggggctct 3720
gtatctatct aggatggata ttccaagcaa tttactctct tgagtgctgt tggtcttcct 3780
ctgctcctcg tctccgcttc ctctcagtct cctccattgc gctgcctgga acagatcgat 3840
ccgccttgtt gctgatctca tccatcctcc gtggagattt cgggtggcag tttccagtga 3900
ctattcagag caccatacta gtggcctggt aaaaatttcc ctcccatctt tcttgctatt 3960
ctcttcttat taatctgctg atttgcttga tggtaagttc ttacttattc ttccagatcg 4020
agtgaagcga catccgccgc cgtcttgtcc cctacgacgc cgctgtgttc tgctctgtcc 4080
tcctctgccg gtgaagcatc aacggtgacg gccatccggt gttgacgccg cggctgtgtt 4140
ctcctctgcc gtgaagtatc aaaggtgggt ttggatggat atcacagttt ggattggcaa 4200
ctctgttcta tagctcgttg aaattaaagc ttcacacagt tttgattgga ttacagctcc 4260
ccatattcat ctattttgca ccatgcatcg aatccactga cctccacacg ctcacggatc 4320
tggcctctgg tgcccacacg ctcacggatc cgacagagtg ctcccttcct atcatctcag 4380
tcctcatctc ctggtcgatc cggcttcttg tttggtgagc tcgtccaacc tccgagctcg 4440
cccctgacac ctgtggagtg gaggtatatt tctttccagc tgaagtacgc ttggatctaa 4500
aaacttacgt ctcctgcttc actgcttgct tgggagtctg atcgatttgt gttggttgaa 4560
tcatttgggg tggcagtgga gcagctagca agaatcatgg agtttccaat cattattcag 4620
agcaccacgc atggaatcca ctgacctggt aactttgtct catctttctt gctactgaaa 4680
tcttccacat ccccaccttc cttgttcatc tgctgatttc ccagattata cttccagatt 4740
gagtgaagcg ccgtcttgtc ccctcacgga tctggcctct ggtgcccagc cgccgctttc 4800
gcttcgttgt ccgacgaggc caccgccgct gtgttctgct ctgccgtgaa gcacaaaaag 4860
gtggggtctt cctctgctct ttctccactg acggcgatcc gaccttgaca cccgtggccg 4920
tggcagtggg gtcttcctct gctctgtttt tttaccagtt attggatcta aaaagggatc 4980
taaaagttat tacttctcct gcttgagatt ctgaccgatt tgtgttgatt gaatccaggg 5040
tggggtcgtg gggctctgta tctatctagt acggagtacc actgtattcc aagcaattta 5100
ctctcttgac tgctgttgga cttcctctgt ccccgcttcc attgtgctgc cttgaacaga 5160
tccatccacc ttgttgctaa tctcatccaa cctccgggta tgtttcttgt cgatttgttt 5220
ccagtaaagt agcaatgctt gggtctaaaa agtcattgct tctcctgctt gacgagtctc 5280
attgatttgc ccagtcatta ttcagagcac catgcatcca aggcgctgac ccggctataa 5340
gtttttatta cagggccgcc gtgttgtccc catcgagcaa agcgtttaat ccactgaccc 5400
gcttcgttgt ccgcacagcg tgaccatccg acgaagacgc cgccgttgtg ttctgctctc 5460
ctctgaagca tcaaaaggtg ggcttggtcc agatccccac attcatcgat ttggcttgcc 5520
aactcagttc caccagagct tggattgtgt tacagatccc taaattcatc gatttgcagg 5580
tttcagctga ttgatcaaga gagctctcat ggcggaagtt ctggtgagcg catccacagg 5640
ggcaatgggt tccctcctgc ggaagttggg agctatgttg acggatgaat acaagctgct 5700
caagaacgtc cgtggggaca tcaagttcct caaggacgag ctggaggtca tgtgcgcgtt 5760
cctcctcaag atgtcagatg tggaggagcc tgacgaacca acaaagctgc gtgtgacggc 5820
ggtgcgggag atgtcctata agatagagga caacatcgac aagttcatgg tcctcgtgga 5880
acaggaacac gggtcctcct gttccgaggc ggctcatggc gtcgccaagc tcatggacaa 5940
gtgcaagaac ttgctgccgg acatcaaggc ccgccgcagg atcgccaagg aggtcaaaga 6000
catcaagaaa gaaattaagg atgtcagcga cagattttca aggtaccaga tcttgttctc 6060
cttacagtac tattagtacc taccatcaga tttgtgaccg atttgttgcc actcaaggta 6120
caagattgac gactcctcct cctctatgcc ggcaaaagac aaggtcgacc ctcgacttcg 6180
tgcagtctac aaagacgccg cagagctcgt cggaattgat ggaccaaagg atgaacttgt 6240
caagtggctg aatgagaaag aaggccaatc actgaaatct gtctctattg ttggatatgg 6300
agggttgggc aagaccacgc tcgccaacca gatccgggtc aaccttggag cgaccttcga 6360
ctgcggggct tttgtctcga tttcacgcaa gcctgacatg aaggcgatat taagatctat 6420
attatcacaa atcaccaaga aagacgatgc ttgctctaga ttagatgata tacaactcat 6480
catcgacaag attcgagaat tcctccaaga cacaaggtat gtatgtgaga tctgctacga 6540
agtaacattg cttaattagc tcatgcataa tcggttgcct agaattttgc taatgaagat 6600
tgctttgtgt gatttacttt cgtcctcaac ttgtatgact gcgcttaaac tttcaagtca 6660
ttgtttcttg caacttatgt cgatctgttc actaattaag atatagctaa aatgtgtttg 6720
ccgatttctg tcgccacaca ttttctaatt tgattgccac aagtttgtta aacttgcggc 6780
tatgttctgt ttattaaata attgtgacat ggcgtcagtc aaaacaataa agtacatgca 6840
attccctctc ttttgtacaa gataaatatc cgcacgcact acatgactgc aaatatatct 6900
atcttctaag gacccaaagc acttgcatgt gcagaccatt gttaccggat atgcagtaca 6960
ttaggtttgt gattagccac tttaagaaca tagggtttct agaggctata aacctacggt 7020
tatcagtatt ataatcttga atggatatca aacatccgtt gaagaatcta tgcccataac 7080
acaaaaagag aaaagggaag aattaatcat agtcagatcg gaccagcctg actagcgtaa 7140
cataatgaca acagtaggtt gttgcgtctt tcctggtttc gtagcaattt gcatgacgaa 7200
ttgaggcgca gagccaccac tcaatatgtg gtcctaccaa tcatgaggaa ataacttgtt 7260
accaaaatga caaaagaaaa taggtagtcc ccatcattgt ttcaatatct atcctcttgc 7320
gaacatgcta ttggcagtgg caaaacattg taccttgcca ctggcaacag ctgcctttac 7380
tcggcctgag cattcatatt tttttcctct tggttgttaa atcttgctat ttttcgcttg 7440
ccttctatgg caatcttttt ttttcttata ttgccaccaa attgtatcta agagcaactc 7500
caacacgccg acgcaatttt tgtccattta gatcggccaa gcggacgggg tgtctggttt 7560
tgcctttggg tggtgcgcct acgcattagt ccgcgtgggc atttaaaaaa cttaaaaagc 7620
ttaaaataat taaatataaa taaaaatgca tattaaagcc cgtcaaagtc cacataatta 7680
aacataatac attaatatat taataaaaaa cagccgcccc agcccgcagc cctaggcgtc 7740
ttcgtcggag gagtgggtga ggtcgaagaa tgccggcagc tcagcccacg ggaaggcctg 7800
ctggtgggca gccagcaccg cctgctccgc tgtctgcggc ggcggcggcc ctacctggca 7860
ccgcgcacgc ctcctcctcg cgctcctgcc gctcctcctc ctcatcgcac gcccaacgaa 7920
gttttggcgc tcctccccct cgggttcccg ctgcatgcgg acctggccgt cgtaccgctc 7980
ctcctccagc gtgcggtcct tccacgccac gaggtaggcc gcctcctcct ccatcacgca 8040
cagccaaatg ggcggcttgc tcatccgctt gcagacgatg ccctgccatt ggtaaacgcc 8100
ggtgggcgcg ggtggtgcta gcgctggtgc aggcggtgac gatgtggctg gtggtcccgg 8160
caggtagaca ctatagctgg cggccgatag ctgcagcgtg tcatcgaggc cagaccacta 8220
ggagtcctcg cactaagact cctcggtgac gtgatgggcc ccctcctcca atgccttgag 8280
gaacgccgcc tcctcctcaa cctactattt gaccaccggt ggcggcggtg gagggcaggc 8340
ttcgagctgg acggcgtcgc ggaggcgcac atggtgctcg atggtgaacc agacttctca 8400
gttaggggag tctggcctcg atgggtggag tcgcgacgct gctctggcgt caggagagca 8460
cgccgggccc gaatctcgtt ggcgcgcgca cgctcggact gtggcaccgc cggcactgga 8520
atgtggtggg ggtcgaggtg ccagccgtgg gggaagttca cgtccggcca cggcagtgag 8580
acgcggtact cccggtggta ctacgcgtgg tgcaccggga tgtactggtg gtcccgtggc 8640
gctgccgccc tcccatcccg cagcgccgac accaatgcga accccttcgc tgccggcctc 8700
accggccctt gcaacgagcc ggcctcgtgg tcgtgcttgc cttgccctta cctttgccaa 8760
agaaacccat gacggctagg gttttctgtc accggcgagg gagcagatgt ggactagagt 8820
gggaggagaa gtggatgagg cccgccccgc cgcacccttg caattaaaaa gggcgtgatc 8880
cggacgtttc tagtcactgc catgcgggcc cgaggtaggt gggcgccatt gactacgtct 8940
gtggtggtag ttggctggca gacacgccag cccgaggcgc acaccgagcg gacgcgcaac 9000
acgtccgtct cgtgttcgtg tgaacgcaaa tcaggcacaa atttgggctg gaaatgcgtc 9060
tacatggatg ccgaacggac gtgatttggg tttgtgtccg cacgttgggc cggcgttttt 9120
gtgcctttcg accctaatgg acacacacgg accaaatccg ttggcgtgtt agagttgctt 9180
gtacctagta ctactatgtg tggagttgtt atctttgagt ttctccccca tctgtagctg 9240
tgtattattg tgagatgctc tcaggatttc acttatttta gataatgaca actttcttga 9300
tgctagacaa ttacatcaag gtgtattata tctaggatgc acacagatgt gagattgttt 9360
caggatttta ggttacttgg taggtgttac tataaatatc taatgagctc taatgtgtat 9420
gttcatgaaa ctgtgttgca ggtactttat cataattgat gatatatggg agttaggaac 9480
atgggaaact ttgaaatgtg catttgtcaa gaatacattg ggcagcagaa taattatcac 9540
aacacgtata gttgatgttg ccaaatcttg ttctccttct agtgaagatc ttgtctatga 9600
gatgaaacca ctcagtgagg ctgactcgaa gaaattgttt ttcaagagga tatttggctg 9660
tgaagaaagc tgtcctgata gcttgaaaga agctgccaat gatattttga aaaaatgccg 9720
tggcctacct ctggccatca atgccatttc tagcgtgttg gtcacaacaa gggaaacaaa 9780
agaagagtgg gatcgggtgc gacattcaat acgctcttca aaggtcaaaa gtgatataat 9840
agagaccatg aattacatat tgtctcttag ttattttgat cttccccacc acctaagaag 9900
ctgcttattg tacctggctc tgtttcctga agaccaattg attggaagga agcggttagt 9960
acgcagatgg atttctgaag gctttatcca tggagaaagt ggacaagatc tcatggaatt 10020
aggagaggaa tattttcacc agcttgtgaa cagaagtcta atacagcccg gcaacatagg 10080
gtatgacggc aaggcaatgt attgccgagt ccatgacacc atccttgatt tcctaattga 10140
caaatcctct gaagagaaca tgtgtactgt cctaaagaaa caatgcaagc ctaatggcat 10200
agttcgtcgg ctctctctaa tggggaatga ggatgaagaa atcgtggagc aattggatct 10260
gtcacacgct cggtcaatca ctgcttttgg ggatatcaag ctattgcctt cgcttgggag 10320
gtcaaaatgc ttgcgcgtgc tagacttgca agactgcgat cagttggaaa atcatcatat 10380
caaggatatt gaaagactct atcagctaag atacttggat atctcttcta caggaatcac 10440
ggagcttccc aggcaaattg gggagttgct gtatctagag acgctagttg cctatggatt 10500
acgtgagctt cctgaaagca caagtcggct acaacgactg gcacgtttgt ttgtttactc 10560
tggttgtaag cttccaggtg ggttgggaaa cttgataaac ttgcaagagc ttgactgcgt 10620
tgatgccttg catttgaagc atgtggaaga gctcggcaaa ctaacaaatc tgagaaagct 10680
gagtattaaa ttggacactg gtgggattga aggcaacaaa ttagaagagt ccaaggaaaa 10740
gctggtgtcc tctctctgta aactggacga atgcggcctc ctttccctga gtattgatta 10800
ttatctgaga gaaaaggatg gagaagagcc gttcctccct gctttgggtt gtatccagga 10860
ggtttttgta tatggacaag atatcagccg gattagcaga tggctcgcgt cacttcccaa 10920
cctacacagg ttgcttttgg acgatccgaa gatagagcag caggatattg agatgattgg 10980
attgataccc aatctgatcg atttgacact gccgcctcta tacaaaacag acgacgctgg 11040
gcggctcatc atcaggcgtg aaggatttca acagttgcag aagtttgagg cttacaatac 11100
tcgtatgggg gttttaatgt ttgaaccggg ggctatgccg aggctcaagg agcttaaact 11160
ccataacttc atcgaaaagc ccaaatctgc tgcggtcgat ttcgactttg gcatccaacg 11220
cctctccagc cttgctcgcc tcactgtcag cttatcctgc ggtggctgga cggtggcaga 11280
agtagaggct gcggaggatg ctttcaagag catggccgag gcgaacccca accgtccaat 11340
actggagatg acaagatata atacgcagca tatgttacaa gacgagcaaa taggtatgac 11400
aggctcaggt actattaact agtttgtact tttgtcttgg tgagcagtag atcaaaagat 11460
gtgcatatgc ttcaatcttt tttccggtaa tctatttcag ctacaacgcc ggccgtcaag 11520
tcctagctga cgttgcttca ggtgtgctct caatctatac atatttactc atcatattat 11580
tctccacctt tttttccatc tctagagctc agcttgtcat tgcattgttc aattgtgctt 11640
ctctagcagc tacggaaatt ctgaacaggc aaaggtaatt ttacagaagt ttctttggca 11700
taagtatctg agaaaaatgc acttggcaca atttaccttt tcgcagtaga aaagagtcag 11760
tggatcatac cttcttaatt gttcgattgc tcgtaatact tgggggcact ctatgattgc 11820
tgtgtgcttt tagttttgat atagttcctg ttagcatgga cagactgtgt gattggttca 11880
tgaatttcag ggtggttcta atgaggaaat gatgcttgct tcagaaatag tttccatttg 11940
tacctaagct gtgtagttgg taaagttgca ttttggatgt tctgttggta tagcttacag 12000
aagaaagact tgcgttgtgc aggtaccagg ggcgcaggtt actgctagtg gcgtctgaga 12060
tgtttcataa acgttgggtg ggcaccggtc atgcaaaaaa ttgaaggata aaaaggttcc 12120
gagaggttgt gtgatgtgct ccttttaaat ggcagtcgct tgcataaaga gatgttgctt 12180
ggttttttag taaagcgttg tagtaagttg ctctgtttcg gctgagagcc atcgttgttg 12240
ctgtctgtta catcgaatgg ctggctcatg tcatgttgat gtttttttta tctgaaccgg 12300
acaaacctga gggtgtagta tcatgtttgt aatgactgaa attctgggct cacccgagct 12360
ccttaattcg taaacaactg cgactcattg ttggattcct tacttcagaa aatatttact 12420
tgctctggct catacagctc tgcatgtttt gtagtggttt gtcgctgttt aggtgctgct 12480
tcagactcct ggtacctttg gccgctgctg ctactgcatg agctttgtat ggcattgtta 12540
gtatagtttg gtgtcattgg gttttcgccc catggtgagt gtccattgcg cgcactcgca 12600
gcggtttcct ggcgataagt tgaacttgat ctttctcttt cccacctcct aaacgtctat 12660
tcagacttgt gtatatttgc attacgggga ttacccgagt tcaaaaataa atattattta 12720
ctagttctgc caatgtgttt ccgttacata caaataaaag ggtgcttcca gctacaagta 12780
tatttttgag aagggccatt gcaaaacgaa atgaagccca aattctacca acatgtttct 12840
gtcacatata cattaaaaaa acggtgtaaa tgtcccgtca aaaaagggtg tatgtaggaa 12900
aaggagttcg acgccgccag ctccctccta acgctcagat tgtcttcgcg gccgccaccg 12960
cggctgagtg gctccttcaa gcttgcggat ccgggcggaa ataggtaaag aagttgcgga 13020
taaggtaatt gccattgcag attatttgga ttgagagtga atatgagact ctaattggat 13080
accgagggga atttatggaa cgtcagtgga gcatttttga caagaaatat ttgctagctg 13140
atagtgacct taggcgactt ttgaacgcgc aataatggtt tctgacgtat gtgcttagct 13200
cattaaactc cagaaacccg cggctgagtg gctccttcaa cgttgcggtt ctgtcagttc 13260
caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt 13320
ctccgctcat gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag 13380
gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa 13440
aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc 13500
ccctcgggag tgcttggcat tccgtgcgat aatgacttct gttcaaccac ccaaacgtcg 13560
gaaagcctga cgacggagca gcattccaaa aagatccctt ggctcgtctg ggtcggctag 13620
aaggtcgagt gggctgctgt ggcttgatcc ctcaacgcgg tcgcggacgt agcgcagcgc 13680
cgaaaaatcc tcgatcgcaa atccgacgct gtcgaaaagc gtgatctgct tgtcgctctt 13740
tcggccgacg tcctggccag tcatcacgcg ccaaagttcc gtcacaggat gatctggcgc 13800
gagttgctgg atctcgcctt caatccgggt ctgtggcggg aactccacga aaatatccga 13860
acgcagcaag atcgtcgacc cctttccgac gctcaccggg ctggttgccc tcgccgctgg 13920
gctggcggcc gtctatggcc ctgcaaacgc gccagaaacg ccgtcgaagc cgtgtgcgag 13980
acaccgcggc cggccgccgg cgttgtggat acctcgcgga aaacttggcc ctcactgaca 14040
gatgaggggc ggacgttgac acttgagggg ccgactcacc cggcgcggcg ttgacagatg 14100
aggggcaggc tcgatttcgg ccggcgacgt ggagctggcc agcctcgcaa atcggcgaaa 14160
acgcctgatt ttacgcgagt ttcccacaga tgatgtggac aagcctgggg ataagtgccc 14220
tgcggtattg acacttgagg ggcgcgacta ctgacagatg aggggcgcga tccttgacac 14280
ttgaggggca gagtgctgac agatgagggg cgcacctatt gacatttgag gggctgtcca 14340
caggcagaaa atccagcatt tgcaagggtt tccgcccgtt tttcggccac cgctaacctg 14400
tcttttaacc tgcttttaaa ccaatattta taaaccttgt ttttaaccag ggctgcgccc 14460
tggcgcgtga ccgcgcacgc cgaagggggg tgccccccct tctcgaaccc tcccggcccg 14520
ctaacgcggg cctcccatcc ccccaggggc tgcgcccctc ggccgcgaac ggcctcaccc 14580
caaaaatggc agccaagctc ctaacatttt attagagagc aggctagttg cttagataca 14640
tgatcttcag gccgttatct gtcagggcaa gcgaaaattg gccatttatg acgaccaatg 14700
ccccgcagaa gctcccatct ttgccgccat agacgccgcg cccccctttt ggggtgtaga 14760
acatcctttt gccagatgtg gaaaagaagt tcgttgtccc attgttggca atgacgtagt 14820
agccggcgaa agtgcgagac ccatttgcgc tatatataag cctacgattt ccgttgcgac 14880
tattgtcgta attggatgaa ctattatcgt agttgctctc agagttgtcg taatttgatg 14940
gactattgtc gtaattgctt atggagttgt cgtagttgct tggagaaatg tcgtagttgg 15000
atggggagta gtcataggga agacgagctt catccactaa aacaattggc aggtcagcaa 15060
gtgcctgccc cgatgccatc gcaagtacga ggcttagaac caccttcaac agatcgcgca 15120
tagtcttccc cagctctcta acgcttgagt taagccgcgc cgcgaagcgg cgtcggcttg 15180
aacgaattgt tagacattat ttgccgacta ccttggtgat ctcgcctttc acgtagtgaa 15240
caaattcttc caactgatct gcgcgcgagg ccaagcgatc ttcttgtcca agataagcct 15300
gcctagcttc aagtatgacg ggctgatact gggccggcag gcgctccatt gcccagtcgg 15360
cagcgacatc cttcggcgcg attttgccgg ttactgcgct gtaccaaatg cgggacaacg 15420
taagcactac atttcgctca tcgccagccc agtcgggcgg cgagttccat agcgttaagg 15480
tttcatttag cgcctcaaat agatcctgtt caggaaccgg atcaaagagt tcctccgccg 15540
ctggacctac caaggcaacg ctatgttctc ttgcttttgt cagcaagata gccagatcaa 15600
tgtcgatcgt ggctggctcg aagatacctg caagaatgtc attgcgctgc cattctccaa 15660
attgcagttc gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg 15720
tgacttctac agcgcggaga atctcgctct ctccagggga agccgaagtt tccaaaaggt 15780
cgttgatcaa agctcgccgc gttgtttcat caagccttac ggtcaccgta accagcaaat 15840
caatatcact gtgtggcttc aggccgccat ccactgcgga gccgtacaaa tgtacggcca 15900
gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac ctctgatagt tgagtcgata 15960
cttcggcgat caccgcttcc ctcatgatgt ttaactcctg aattaagccg cgccgcgaag 16020
cggtgtcggc ttgaatgaat tgttaggcgt catcctgtgc tcccgagaac cagtaccagt 16080
acatcgctgt ttcgttcgag acttgaggtc tagttttata cgtgaacagg tcaatgccgc 16140
cgagagtaaa gccacatttt gcgtacaaat tgcaggcagg tacattgttc gtttgtgtct 16200
ctaatcgtat gccaaggagc tgtctgctta gtgcccactt tttcgcaaat tcgatgagac 16260
tgtgcgcgac tcctttgcct cggtgcgtgt gcgacacaac aatgtgttcg atagaggcta 16320
gatcgttcca tgttgagttg agttcaatct tcccgacaag ctcttggtcg atgaatgcgc 16380
catagcaagc agagtcttca tcagagtcat catccgagat gtaatccttc cggtaggggc 16440
tcacacttct ggtagatagt tcaaagcctt ggtcggatag gtgcacatcg aacacttcac 16500
gaacaatgaa atggttctca gcatccaatg tttccgccac ctgctcaggg atcaccgaaa 16560
tcttcatatg acgcctaacg cctggcacag cggatcgcaa acctggcgcg gcttttggca 16620
caaaaggcgt gacaggtttg cgaatccgtt gctgccactt gtttaataga ctggatggag 16680
gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct 16740
gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat 16800
ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa 16860
cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac 16920
caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 16980
taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 17040
cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgatatcc tttttttctg 17100
cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 17160
gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 17220
aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 17280
cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 17340
tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 17400
acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 17460
ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 17520
ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 17580
tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 17640
tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 17700
ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 17760
gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag 17820
cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg 17880
catctgtgcg gtatttcaca ccgcatatga agatcggcgg caatagcttc ttagcgccat 17940
cccggctgag aaagcccagt aaggaaacaa ctgtaggttc gagtcgcgag atcccccgga 18000
accaaaggaa gtaggttaaa cccgctccga tcaggccgag ccacgccagg ccgagaacat 18060
tggttcctgt aggcatcggg attggcggat caaacactaa agctactgga acgagcagaa 18120
gtcctccggc cgccagttgc caggcggtaa aggtgagcag aggcacggga ggttgccact 18180
tgcgggtcag cacggttccg aacgccatgg aaaccgcccc cgccaggccc gctgcgacgc 18240
cgacaggatc tagcgctgcg tttggtgtca acaccaacag cgccacgccc gcagttccgc 18300
aaatagcccc caggaccgcc atcaatcgta tcgggctacc tagcagagcg gcagagatga 18360
acacgaccat cagcggctgc acagcgccta ccgtcgccgc gaccccgccc ggcaggcggt 18420
agaccgaaat aaacaacaag ctccagaata gcgaaatatt aagtgcgccg aggatgaaga 18480
tgcgcatcca ccagattccc gttggaatct gtcggacgat catcacgagc aataaacccg 18540
ccggcaacgc ccgcagcagc ataccggcga cccctcggcc tcgctgttcg ggctccacga 18600
aaacgccgga cagatgcgcc ttgtgagcgt ccttggggcc gtcctcctgt ttgaagaccg 18660
acagcccaat gatctcgccg tcgatgtagg cgccgaatgc cacggcatct cgcaaccgtt 18720
cagcgaacgc ctccatgggc tttttctcct cgtgctcgta aacggacccg aacatctctg 18780
gagctttctt cagggccgac aatcggatct cgcggaaatc ctgcacgtcg gccgctccaa 18840
gccgtcgaat ctgagcctta atcacaattg tcaattttaa tcctctgttt atcggcagtt 18900
cgtagagcgc gccgtgcgtc ccgagcgata ctgagcgaag caagtgcgtc gagcagtgcc 18960
cgcttgttcc tgaaatgcca gtaaagcgct ggctgctgaa cccccagccg gaactgaccc 19020
cacaaggccc tagcgtttgc aatgcaccag gtcatcattg acccaggcgt gttccaccag 19080
gccgctgcct cgcaactctt cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg 19140
ggtggaatcc gatccgcaca tgaggcggaa ggtttccagc ttgagcgggt acggctcccg 19200
gtgcgagctg aaatagtcga acatccgtcg ggccgtcggc gacagcttgc ggtacttctc 19260
ccatatgaat ttcgtgtagt ggtcgccagc aaacagcacg acgatttcct cgtcgatcag 19320
gacctggcaa cgggacgttt tcttgccacg gtccaggacg cggaagcggt gcagcagcga 19380
caccgattcc aggtgcccaa cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg 19440
cgacaggcat tcctcggcct tcgtgtaata ccggccattg atcgaccagc ccaggtcctg 19500
gcaaagctcg tagaacgtga aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc 19560
caacacctgc tgccacacca gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt 19620
gatcttcacg tccttgttga cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat 19680
tttcttgttg cgcgtggtga acagggcaga gcgggccgtg tcgtttggca tcgctcgcat 19740
cgtgtccggc cacggcgcaa tatcgaacaa ggaaagctgc atttccttga tctgctgctt 19800
cgtgtgtttc agcaacgcgg cctgcttggc ctcgctgacc tgttttgcca ggtcctcgcc 19860
ggcggttttt cgcttcttgg tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc 19920
caaacctgcc gcctcctgtt cgagacgacg cgaacgctcc acggcggccg atggcgcggg 19980
cagggcaggg ggagccagtt gcacgctgtc gcgctcgatc ttggccgtag cttgctggac 20040
catcgagccg acggactgga aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat 20100
ggtttcggca tcctcggcgg aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg 20160
gtcaaacgtc cgattcattc accctccttg cgggattgcc ccgactcacg ccggggcaat 20220
gtgcccttat tcctgatttg acccgcctgg tgccttggtg tccagataat ccaccttatc 20280
ggcaatgaag tcggtcccgt agaccgtctg gccgtccttc tcgtacttgg tattccgaat 20340
cttgccctgc acgaatacca gctccgcgaa gtcgctcttc ttgatggagc gcatggggac 20400
gtgcttggca atcacgcgca ccccccggcc gttttagcgg ctaaaaaagt catggctctg 20460
ccctcgggcg gaccacgccc atcatgacct tgccaagctc gtcctgcttc tcttcgatct 20520
tcgccagcag ggcgaggatc gtggcatcac cgaaccgcgc cgtgcgcggg tcgtcggtga 20580
gccagagttt cagcaggccg cccaggcggc ccaggtcgcc attgatgcgg gccagctcgc 20640
ggacgtgctc atagtccacg acgcccgtga ttttgtagcc ctggccgacg gccagcaggt 20700
aggccgacag gctcatgccg gccgccgccg ccttttcctc aatcgctctt cgttcgtctg 20760
gaaggcagta caccttgata ggtgggctgc ccttcctggt tgggtaatga ctccaactta 20820
ttgatagtgt tttatgttca gataatgccc gatgactttg tcatgcagct ccaccgattt 20880
tgagaacgac agcgacttcc gtcccagccg tgccaggtgc tgcctcagat tcaggttatg 20940
ccgctcaatt cgctgcgtat atcgcttgct gattacgtgc agctttccct tcaggcggga 21000
ttcatacagc ggccagccat ccgtcatcca tatcaccacg tcaaagggtg acagcaggct 21060
cataagacgc cccagcgtcg ccatagtgcg ttcaccgaat acgtgcgcaa caaccgtctt 21120
ccggagactg tcatacgcgt aaaacagcca gcgctggcgc gatttagccc cgacatagcc 21180
ccactgttcg tccatttccg cgcagacgat gacgtcactg cccggctgta tgcgcgaggt 21240
taccgactgc ggcctgagtt ttttaagtga cgtaaaatcg tgttgaggcc aacgcccata 21300
atgcgggctg ttgcccggca tccaacgcca ttcatggcca tatcaatgat tttctggtgc 21360
gtaccgggtt gagaagcggt gtaagtgaac tgcagttgcc atgttttacg gcagtgagag 21420
cagagatagc gctgatgtcc ggcggtgctt ttgccgttac gcaccacccc gtcagtagct 21480
gaacaggagg gacagctgat agaaacagaa gccactggag cacctcaaaa acaccatcat 21540
acactaaatc agtaagttgg cagcatcacc cctggttggc ttggtttcat cagccatccg 21600
cttgccctca tctgttacgc cggcggtagc cggccagcct cgcagagcag gattcccgtt 21660
gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa cacccgctcg cgggtgggcc 21720
tacttcacct atcctgcccg gctgacgccg ttggatacac caaggaaagt ctacacgaac 21780
cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa aaatcgctat 21840
aatgaccccg aagcagggtt atgcagcgga aaagatcc 21878
<210> 158
<211> 21887
<212> DNA
<213> 人工序列
<220>
<223> 转化构建体
<400> 158
gtcgacgatc gtcaacgttc acttctaaag aaatagcgcc actcagcttc ctcagcggct 60
ttatccagcg atttcctatt atgtcggcat agttctcaag atcgacagcc tgtcacggtt 120
aagcgagaaa tgaataagaa ggctgataat tcggatctct gcgagggaga tgatatttga 180
tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc 240
cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca 300
aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg 360
tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca 420
ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt 480
ttttaatgta ctgaattcca atccccaaaa atctgagctt aacagcacag ttgctcctct 540
cagagcagaa tcgggtattc aacaccctca tatcaactac tacgttgtgt ataacggtcc 600
acatgccggt atatacgatg actggggttg tacaaaggcg gcaacaaacg gcgttcccgg 660
agttgcacac aagaaatttg ccactattac agaggcaaga gcagcagctg acgcgtacac 720
aacaagtcag caaacagaca ggttgaactt catccccaaa ggagaagctc aactcaagcc 780
caagagcttt gctaaggccc taacaagccc accaaagcaa aaagcccact ggctcacgct 840
aggaaccaaa aggcccagca gtgatccagc cccaaaagag atctcctttg ccccggagat 900
tacaatggac gatttcctct atctttacga tctaggaagg aagttcgaag gtgaaggtga 960
cgacactatg ttcaccactg ataatgagaa ggttagcctc ttcaatttca gaaagaatgc 1020
tgacccacag atggttagag aggcctacgc agcaggtctc atcaagacga tctacccgag 1080
taacaatctc caggagatca aataccttcc caagaaggtt aaagatgcag tcaaaagatt 1140
caggactaat tgcatcaaga acacagagaa agacatattt ctcaagatca gaagtactat 1200
tccagtatgg acgattcaag gcttgcttca taaaccaagg caagtaatag agattggagt 1260
ctctaaaaag gtagttccta ctgaatctaa ggccatgcat ggagtctaag attcaaatcg 1320
aggatctaac agaactcgcc gtgaagactg gcgaacagtt catacagagt cttttacgac 1380
tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca cgacactctg gtctactcca 1440
aaaatgtcaa agatacagtc tcagaagacc aaagggctat tgagactttt caacaaagga 1500
taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc atcgaaagga 1560
cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggctatca 1620
ttcaagatct ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg 1680
tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gacatctcca 1740
ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc tctatataag 1800
gaagttcatt tcatttggag aggacacgct cgagtataag agctctattt ttacaacaat 1860
taccaacaac aacaaacaac aaacaacatt acaattacta tttacaatta ccatgagccc 1920
agaacgacgc ccggccgaca tccgccgtgc caccgaggcg gacatgccgg cggtctgcac 1980
catcgtcaac cactacatcg agacaagcac ggtcaacttc cgtaccgagc cgcaggaacc 2040
gcaggagtgg acggacgacc tcgtccgtct gcgggagcgc tatccctggc tcgtcgccga 2100
ggtggacggc gaggtcgccg gcatcgccta cgcgggcccc tggaaggcac gcaacgccta 2160
cgactggacg gccgagtcaa ccgtgtacgt ctccccccgc caccagcgga cgggactggg 2220
ctccacgctc tacacccacc tgctgaagtc cctggaggca cagggcttca agagcgtggt 2280
cgctgtcatc gggctgccca acgacccgag cgtgcgcatg cacgaggcgc tcggatatgc 2340
cccccgcggc atgctgcggg cggccggctt caagcacggg aactggcatg acgtgggttt 2400
ctggcagctg gacttcagcc tgccggtacc gccccgtccg gtcctgcccg tcaccgagat 2460
ttgatgaccc gggggatcct agagtcctgc tttaatgaga tatgcgagac gcctatgatc 2520
gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct 2580
cagatcctta ccgccggttt cggttcattc taatgaatat atcacccgtt actatcgtat 2640
ttttatgaat aatattctcc gttcaattta ctgattgtac cctactactt atatgtacaa 2700
tattaaaatg aaaacaatat attgtgctga ataggtttat agcgacatct atgatagagc 2760
gccacaataa caaacaattg cgttttatta ttacaaatcc aattttaaaa aaagcggcag 2820
aaccggtcaa acctaaaaga ctgattacat aaatcttatt caaatttcaa aagtgcccca 2880
ggggctagta tctacgacac accgagcggc gaactaataa cgctcactga agggaactcc 2940
ggttccccgc cggcgcgcat gggtgagatt ccttgaagtt gagtattggc cgtccgctct 3000
accgaaagtt acgggcacca ttcaacccgg tccagcacgg cggccgggta accgacttgc 3060
tgccccgaga attatgcagc atttttttgg tgtatgtggg ccccaaatga agtgcaggtc 3120
aaaccttgac agtgacgaca aatcgttggg cgggtccagg gcgaattttg cgacaacatg 3180
tcgaggctca gcaggacctg caggcatgca agctctagag cggccgcttt aagagtgttg 3240
aggatgtgct tacttaaatt tcaaaagggc tatttaggga taatatttgg tgaagcatgg 3300
ctggtggact gaccataggt acccactgaa taaacatatc agcccaaagg cagagggtat 3360
ccctccctgt aatacgtact agtattcgag gagcgtgcta aagtagtatc ctctcttgat 3420
gctcctcgtc tccctccact gagtactccc ttcctctcct cattctcatc tcagtcctct 3480
cctcctccct cgaataagat agatccgccc ttgttgctga tctcatctcg cctcctattg 3540
gcctcgcccc tgccaactga ctgctctatt ggtgaccgtg gactttttgg gtatatatat 3600
ttcctgtcga tttgtttcca cttaaagtag caatgcttgg atctaaaact tattacttct 3660
cctgcttcag attctgatcc atttgtgttg attgaactca gggtgggatc gtggggctct 3720
gtatctatct aggatggata ttccaagcaa tttactctct tgagtgctgt tggtcttcct 3780
ctgctcctcg tctccgcttc ctctcagtct cctccattgc gctgcctgga acagatcgat 3840
ccgccttgtt gctgatctca tccatcctcc gtggagattt cgggtggcag tttccagtga 3900
ctattcagag caccatacta gtggcctggt aaaaatttcc ctcccatctt tcttgctatt 3960
ctcttcttat taatctgctg atttgcttga tggtaagttc ttacttattc ttccagatcg 4020
agtgaagcga catccgccgc cgtcttgtcc cctacgacgc cgctgtgttc tgctctgtcc 4080
tcctctgccg gtgaagcatc aacggtgacg gccatccggt gttgacgccg cggctgtgtt 4140
ctcctctgcc gtgaagtatc aaaggtgggt ttggatggat atcacagttt ggattggcaa 4200
ctctgttcta tagctcgttg aaattaaagc ttcacacagt tttgattgga ttacagctcc 4260
ccatattcat ctattttgca ccatgcatcg aatccactga cctccacacg ctcacggatc 4320
tggcctctgg tgcccacacg ctcacggatc cgacagagtg ctcccttcct atcatctcag 4380
tcctcatctc ctggtcgatc cggcttcttg tttggtgagc tcgtccaacc tccgagctcg 4440
cccctgacac ctgtggagtg gaggtatatt tctttccagc tgaagtacgc ttggatctaa 4500
aaacttacgt ctcctgcttc actgcttgct tgggagtctg atcgatttgt gttggttgaa 4560
tcatttgggg tggcagtgga gcagctagca agaatcatgg agtttccaat cattattcag 4620
agcaccacgc atggaatcca ctgacctggt aactttgtct catctttctt gctactgaaa 4680
tcttccacat ccccaccttc cttgttcatc tgctgatttc ccagattata cttccagatt 4740
gagtgaagcg ccgtcttgtc ccctcacgga tctggcctct ggtgcccagc cgccgctttc 4800
gcttcgttgt ccgacgaggc caccgccgct gtgttctgct ctgccgtgaa gcacaaaaag 4860
gtggggtctt cctctgctct ttctccactg acggcgatcc gaccttgaca cccgtggccg 4920
tggcagtggg gtcttcctct gctctgtttt tttaccagtt attggatcta aaaagggatc 4980
taaaagttat tacttctcct gcttgagatt ctgaccgatt tgtgttgatt gaatccaggg 5040
tggggtcgtg gggctctgta tctatctagt acggagtacc actgtattcc aagcaattta 5100
ctctcttgac tgctgttgga cttcctctgt ccccgcttcc attgtgctgc cttgaacaga 5160
tccatccacc ttgttgctaa tctcatccaa cctccgggta tgtttcttgt cgatttgttt 5220
ccagtaaagt agcaatgctt gggtctaaaa agtcattgct tctcctgctt gacgagtctc 5280
attgatttgc ccagtcatta ttcagagcac catgcatcca aggcgctgac ccggctataa 5340
gtttttatta cagggccgcc gtgttgtccc catcgagcaa agcgtttaat ccactgaccc 5400
gcttcgttgt ccgcacagcg tgaccatccg acgaagacgc cgccgttgtg ttctgctctc 5460
ctctgaagca tcaaaaggtg ggcttggtcc agatccccac attcatcgat ttggcttgcc 5520
aactcagttc caccagagct tggattgtgt tacagatccc taaattcatc gatttgcagg 5580
tttcagctga ttgatcaaga gagctctcaa tgatggcgga agttctggtg agcgcatcca 5640
caggcgcaat gggttccctc ctccggaagt tgggagctat gttgacggat gaatacaagc 5700
tgctcaagaa cgtccgtggg gacatcaagt tcctcaagga cgagctggag gtcatgtgcg 5760
cgttcctcct caagatgtca gatgtggagg agcctgacga accaacaaag ctgcgtgtga 5820
cggcggtgcg ggagatgtcc tataagatag aggacaacat cgacaagttc atggtcctcg 5880
tggaacacga gtcctcctgc tccgaggcgg ctcatggcgt cgccaagctc atggacaagt 5940
gcaagaactt gctgccggac atcaagaccc gccgcaggat cgccaaggag gtcaaagaca 6000
tcaagaaaga aattaaggat gtcagcgaca gattttcaag gtaccagatc ttgttctctt 6060
tacagtacta ttagtaccta ccatcagatt tgtgaccgat ttgttgccac tcaaggtaca 6120
agattgacga gtcctcctcc tctatgccgg caaaaggcaa ggtcgaccct cgacttcgtg 6180
cagtctacaa agacgccgca gagctcgtcg gaattgatgg accaaaggat gaacttgtca 6240
agtggctgaa tgagaaagag ggccaatcac tgaaatctgt ctctattgtt ggatatggag 6300
ggttgggcaa gaccacgctc gccaaccaga tccgggtcaa ccttggagcg accttcgact 6360
gcggggcttt tgtctcgatt tcacgcaagc ctgacatgaa ggcgatatta agatctatat 6420
tatcacaaat caccaagaaa gacgatgctt gctctagatt agatgatata caactcatca 6480
tcgacaagat tcgagaattc ctccaagaca caaggtatgt atgtgagatc tgctacgaag 6540
taacattgct taattagctc atgcataatc ggttgcctag aattttgcta atgaagattg 6600
ctttgtgtga tttactttcg tcctcaactt gtatgactgc gcttaaactt tcaagtcatt 6660
gtttcttgca acttatgtcg atctgttcac taattaagat atagctaaaa tgtgtttgcc 6720
gatttctgtc gccacacatt ttctaatttg attgccacaa gtttgttaaa cttgcggcta 6780
tgttctgttt attaaataat tgtgacatgg cgtcagtcaa aacaataaag tacatgcaat 6840
tccctctctt ttgtacaaga taaatatccg cacgcactac atgactgcaa atatatctat 6900
cttctaagga cccaaagcac ttgcatgtgc agaccattgt taccggatat gcagtacatt 6960
aggtttgtga ttagccactt taagaacata gggtttctag aggctataaa cctacggtta 7020
tcagtattat aatcttgaat ggatatcaaa catccgttga agaatctatg cccataacac 7080
aaaaagagaa aagggaagaa ttaatcatag tcagatcgga ccagcctgac tagcgtaaca 7140
taatgacaac agtaggttgt tgcgtctttc ctggtttcgt agcaatttgc atgacgaatt 7200
gaggcgcaga gccaccactc aatatgtggt cctaccaatc atgaggaaat aacttgttac 7260
caaaatgaca aaagaaaata ggtagtcccc atcattgttt caatatctat cctcttgcga 7320
acatgctatt ggcagtggca aaacattgta ccttgccact ggcaacagct gcctttactc 7380
ggcctgagca ttcatatttt tttcctcttg gttgttaaat cttgctattt ttcgcttgcc 7440
ttctatggca atcttttttt ttcttatatt gccaccaaat tgtatctaag agcaactcca 7500
acacgccgac gcaatttttg tccatttaga tcggccaagc ggacggggtg tctggttttg 7560
cctttgggtg gtgcgcctac gcattagtcc gcgtgggcat ttaaaaaact taaaaagctt 7620
aaaataatta aatataaata aaaatgcata ttaaagccca tcaaagtcca cataattaaa 7680
cataatacat taatatatta ataaaaaaca gccgccccag cccgcagccc taggcgtctt 7740
cgtcggagga gtgggtgagg tcgaagaatg ccggcagctc agcccacggg aaggcctgct 7800
ggtgggcagc cagcaccgcc tgctccgctg tctgcggcgg cggcggccct acctggcacc 7860
gcgcacgcct cctcctcgcg ctcctgccgc tcctcctcct catcgcacgc ccaacgaagt 7920
tttggcgctc ctccccctcg ggttcccgct gcatgcggac ctggccgtcg taccgctcct 7980
cctccagcgt gcggtccttc cacgccacga ggtaggccgc ctcctcctcc atcacgcaca 8040
gccaaatggg cggcttgctc atccgcttgc agacgatgcc ctgccattgg taaacgccgg 8100
tgggcgcggg tggtgctagc gctggtgcag gcggtgacga tgtggctggt ggtcccggca 8160
ggtagacact atagttggcg gccgatagct gcagcgtgtc atcgaggcca gaccactagg 8220
agtcctcgca ctaagactcc tcggtgacgt gatgggcccc ctcctccaat gccttgagga 8280
acgccgcctc ctcctcaacc tactatttga ccaccggtgg cggcggtgga gggcaggctt 8340
cgagctggac ggcgtcgcgg aggcgcacat ggtgctcgat ggtgaaccag acttctcagt 8400
taggggagtc tggcctcgat gggtggagtc gcgacgctgc tctggcgtca ggagagcacg 8460
ccgggcccga atctcgttgg cgcgcgcacg ctcggactgt ggcaccgccg gcactggaat 8520
gtggtggggg tcgaggtgcc agccgtgggg gaagttcacg tccggccatg gcagtgagac 8580
gcggtactcc cggtggtact aactacgcgt ggtgcaccgg gatgtactgg tggtcccgtg 8640
gcgctgccgc cctcccatcc cgcagcgccg acaccaatgc gaaccccttc gctgccggcc 8700
tcaccggccc ttgcaacgag ccggcctcgt ggtcgtgctt gccttgccct tacctttgcc 8760
aaagaaaccc atgacggcta gggttttctg tcaccggcga gggagcagat gtggactaga 8820
gtgggaggag aagtggatga ggcccgcccc gccgcaccct tgcaattaaa aagggcgtga 8880
tccggacgtt tctagtcact gccatgcggg cccgaggtag gtgggcggca ttgactacgt 8940
ctgtggtggt agttggctgg cagacacgcc agcccgaggc gcacaccgag cggacgcgca 9000
acacgtccgt ctcgtgttcg tgtgaacgca aatcaggcac aaatttgggc tggaaatgcg 9060
tctacatgga tgccgaacgg acgtgatttg ggtttgtgtc cgcacgttgg gccggcgttt 9120
ttgtgccttt cgaccctaat ggacacacac ggaccaaatc cgttggcgtg ttagagttgc 9180
ttgtacctag tactactatg tgtggagttg ttatctttga gtttctcccc catctgtagc 9240
tgtgtattat tgtgagatgc tctcaggatt tcacttattt tagataatga caactttctt 9300
gatgctagac aattacatca aggtgtatta tatctaggat gcacacagat gtgagattgt 9360
ttcaggattt taggttactt ggtaggtgtt actataaata tctaatgagc tctaatgtgt 9420
atgttcatga aactgtgttg caggtacttt atcataattg atgatatatg ggagttagga 9480
acatgggaaa ctttgaaatg tgcatttgtc aagaatacat tgggcagcag aataattatc 9540
acaacacgta tagttgatgt tgccaaatct tgttctcctt ctagtgaaga tcttgtctat 9600
gagatgaaac cactcagtga ggctgactcg aagaaattgt ttttcaagag gatatttggc 9660
tgtgaagaaa gctgtcctga tagcttgaaa gaagctgcca atgatatttt gaaaaaatgc 9720
cgtggcctac ctctggccat caatgccatt tctagcttgt tggtcacaac aagggaaaca 9780
aaagaagagt gggatcgggt gcgacattca atacgctctt caaaggtcaa aagtgatata 9840
atagaaacca tgaattacat attgtctctt agttattttg atcttcccca ccacctaaga 9900
agctgcttat tgtacctggc tctgtttcct gaagaccaat tgattggaag gaagcggtta 9960
gtacgcagat ggatttctga aggctttatc catggagaaa gtggacaaga tctcatggaa 10020
ttaggagagg aatattttca ccagcttgtg aacagaagtc taatacagcc cggcaacata 10080
gggtatgacg gcaaggcgga gtattgccga gtccatgaca ccatccttga tttcctaatt 10140
gacaaatcct ctgaagagaa catgtgtact gtcctgaaga aacaatgcaa gcctaatggc 10200
atagttcgtc ggctctctct aatggggaat gaggatgaag aaatcatgga gcaattggat 10260
ctgtcacacg ctcggtcaat cagtgctttt ggggatatca agctattgcc ttcgcttggg 10320
aggtcaaaat gcttgcgcgt gctagacttg caaggctgct atcagttgaa aaatcatcat 10380
atcaaggata ttgaaagact ctatcagctg agatacttgg atatctcttt tacaggaatc 10440
acggagcttc ccaggcaaat tggggagttg ctgtatctag agacgctagt tagtacatcc 10500
tatggattac gtgagcttcc tgaaagcaca agtcggctac aacgactggc acgtttgttt 10560
gttgacagcg gttgtaagct tccagatggg ttgggaaact tgataaactt gcaagagctt 10620
gactgcgttg atgccttgca gttgaagcat gtggaagagc tcggcaaact aacaaatctg 10680
agaaagctga ggattaaatt ggacactgat gggattgaag gcaacaaatt agaacagtcc 10740
aaggaaaagc tggtgtcctc tctctgtaaa ctggacgaat gcggcctcct ttccctgagt 10800
atttattatt atctgagaga aaaggatgga gaagagccgt tcctccctgc tttgggttgt 10860
atccaggagg tttttgtaca tggacaagat atcagccgga ttagcagatg gctcgcgtca 10920
cttcccaacc tacacaggtt gtttttgaac caaccgaaga tagagcagca ggatattgag 10980
atgattggat tgatacccaa tctgatggat ttgacactgt atttatgcat aacagacgac 11040
gctgggcggc tcatcatcaa gcgtgaagga tttcaacagt tgcagaggtt tgagctttcc 11100
cgtattcgta tgggggattt aatgtttgaa ccgggggcta tgccgaggct caaggagctt 11160
atactctacg acttcatcgg aaagcccaaa tctggtgcgg tcgatttcga ctttggcatc 11220
caacacctct ccagccttgc tcgcctcact gtcggcttat cctgcgttgg ctcgacggtg 11280
gcggaagtag aggccgcgga ggatgctttc aagagcatgg ccgaggcgaa ccccaaccgt 11340
ccaatactgg agatgacaag atataatacg cagcatatgt tacaagacga gcaaataggt 11400
atgacaggct caggtactat taactagttt gtacttttgt cttggtgagc ggtagatcaa 11460
aagatgtgca tatgcttcaa tcttttttcc ggtaatctat ttcagctaca acgccggccg 11520
tcaagtccta ggcttctgac gttgcttcag gtgtgctctc aatctataca tatttactca 11580
tcatattatt ctccaccttt ttttccatct ctagagctca gcttgtcatt gcattgttca 11640
attgtgcttc tctagcagct acggaaattc tgaacaggca aaggtaattt tacagaagtt 11700
tctttggcat aagtatctga gaaaaatgca cttggcacaa tttacctttt cgcagtagaa 11760
aagagtcagt ggatcatacc ttcttaattg ttcgattgct cgtaatactt gggggcactc 11820
tatgattgct gtgtgctttt agttttgata tagttcctgt tagcatggac agactgtgtg 11880
attggttcat gaatttcagg gtggttctaa tgaggaaatg atgcttgctt cagaaatagt 11940
ttccatttgt acctaagctg tgtagttggt aaagttgcat tttggatgtt ctgttggtat 12000
agcttacaga agaaagactt gcgttgtgca ggtaccaggg gcgcaggtta ctgctagtgg 12060
cgtctgagat gtttcataaa cgttgggtgg gcaccggtca tgcaaaaaat tgaaggataa 12120
aaaggttccg agaggttgtg tgatgtgctc cttttaaatg gcagtcgctt gcataaagag 12180
atgttgcttg gttttttagt aaagcgttgt agtaagttgc tctgtttcgg ctgagagcca 12240
tcgttgttgc tgtctgttac atcgaatggc tggctcatgt catgttgatg ttttttttat 12300
ctgaaccgga caaacctgag ggtgtagtat catgtttgta atgactgaaa ttctgggctc 12360
acccgagctc cttaattcgt aaacaactgc gactcattgt tggattcctt acttcagaaa 12420
atatttactt gctctggctc atacagctct gcatgttttg tagtggtttg tcgctgttta 12480
ggtgctgctt cagactcctg gtacctttgg ccgctgctgc tactgcatga gctttgtatg 12540
gcattgttag tatagtttgg tgtcattggg ttttcgcccc atggtgagtg tccattgcgc 12600
gcactcgcag cggtttcctg gcgataagtt gaacttgatc tttctctttc ccacctccta 12660
aacgtctatt cagacttgtg tatatttgca ttacggggat tacccgagtt caaaaataaa 12720
tattatttac tagttctgcc aatgtgtttc cgttacatac aaataaaagg gtgcttccag 12780
ctacaagtat atttttgaga agggccattg caaaacgaaa tgaagcccaa attctaccaa 12840
catgtttctg tcacatatac attaaaaaaa cggtgtaaat gtcccgtcaa aaaagggtgt 12900
atgtaggaaa aggagttcga cgccgccagc tccctcctaa cgctcagatt gtcttcgcgg 12960
ccgccaccgc ggctgagtgg ctccttcaag cttgcggatc cgggcggaaa taggtaaaga 13020
agttgcggat aaggtaattg ccattgcaga ttatttggat tgagagtgaa tatgagactc 13080
taattggata ccgaggggaa tttatggaac gtcagtggag catttttgac aagaaatatt 13140
tgctagctga tagtgacctt aggcgacttt tgaacgcgca ataatggttt ctgacgtatg 13200
tgcttagctc attaaactcc agaaacccgc ggctgagtgg ctccttcaac gttgcggttc 13260
tgtcagttcc aaacgtaaaa cggcttgtcc cgcgtcatcg gcgggggtca taacgtgact 13320
cccttaattc tccgctcatg atcagattgt cgtttcccgc cttcagttta aactatcagt 13380
gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg 13440
gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc 13500
acagggttcc cctcgggagt gcttggcatt ccgtgcgata atgacttctg ttcaaccacc 13560
caaacgtcgg aaagcctgac gacggagcag cattccaaaa agatcccttg gctcgtctgg 13620
gtcggctaga aggtcgagtg ggctgctgtg gcttgatccc tcaacgcggt cgcggacgta 13680
gcgcagcgcc gaaaaatcct cgatcgcaaa tccgacgctg tcgaaaagcg tgatctgctt 13740
gtcgctcttt cggccgacgt cctggccagt catcacgcgc caaagttccg tcacaggatg 13800
atctggcgcg agttgctgga tctcgccttc aatccgggtc tgtggcggga actccacgaa 13860
aatatccgaa cgcagcaaga tcgtcgaccc ctttccgacg ctcaccgggc tggttgccct 13920
cgccgctggg ctggcggccg tctatggccc tgcaaacgcg ccagaaacgc cgtcgaagcc 13980
gtgtgcgaga caccgcggcc ggccgccggc gttgtggata cctcgcggaa aacttggccc 14040
tcactgacag atgaggggcg gacgttgaca cttgaggggc cgactcaccc ggcgcggcgt 14100
tgacagatga ggggcaggct cgatttcggc cggcgacgtg gagctggcca gcctcgcaaa 14160
tcggcgaaaa cgcctgattt tacgcgagtt tcccacagat gatgtggaca agcctgggga 14220
taagtgccct gcggtattga cacttgaggg gcgcgactac tgacagatga ggggcgcgat 14280
ccttgacact tgaggggcag agtgctgaca gatgaggggc gcacctattg acatttgagg 14340
ggctgtccac aggcagaaaa tccagcattt gcaagggttt ccgcccgttt ttcggccacc 14400
gctaacctgt cttttaacct gcttttaaac caatatttat aaaccttgtt tttaaccagg 14460
gctgcgccct ggcgcgtgac cgcgcacgcc gaaggggggt gccccccctt ctcgaaccct 14520
cccggcccgc taacgcgggc ctcccatccc cccaggggct gcgcccctcg gccgcgaacg 14580
gcctcacccc aaaaatggca gccaagctcc taacatttta ttagagagca ggctagttgc 14640
ttagatacat gatcttcagg ccgttatctg tcagggcaag cgaaaattgg ccatttatga 14700
cgaccaatgc cccgcagaag ctcccatctt tgccgccata gacgccgcgc cccccttttg 14760
gggtgtagaa catccttttg ccagatgtgg aaaagaagtt cgttgtccca ttgttggcaa 14820
tgacgtagta gccggcgaaa gtgcgagacc catttgcgct atatataagc ctacgatttc 14880
cgttgcgact attgtcgtaa ttggatgaac tattatcgta gttgctctca gagttgtcgt 14940
aatttgatgg actattgtcg taattgctta tggagttgtc gtagttgctt ggagaaatgt 15000
cgtagttgga tggggagtag tcatagggaa gacgagcttc atccactaaa acaattggca 15060
ggtcagcaag tgcctgcccc gatgccatcg caagtacgag gcttagaacc accttcaaca 15120
gatcgcgcat agtcttcccc agctctctaa cgcttgagtt aagccgcgcc gcgaagcggc 15180
gtcggcttga acgaattgtt agacattatt tgccgactac cttggtgatc tcgcctttca 15240
cgtagtgaac aaattcttcc aactgatctg cgcgcgaggc caagcgatct tcttgtccaa 15300
gataagcctg cctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg 15360
cccagtcggc agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc 15420
gggacaacgt aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata 15480
gcgttaaggt ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt 15540
cctccgccgc tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag 15600
ccagatcaat gtcgatcgtg gctggctcga agatacctgc aagaatgtca ttgcgctgcc 15660
attctccaaa ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca 15720
caacaatggt gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt 15780
ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa 15840
ccagcaaatc aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat 15900
gtacggccag caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt 15960
gagtcgatac ttcggcgatc accgcttccc tcatgatgtt taactcctga attaagccgc 16020
gccgcgaagc ggtgtcggct tgaatgaatt gttaggcgtc atcctgtgct cccgagaacc 16080
agtaccagta catcgctgtt tcgttcgaga cttgaggtct agttttatac gtgaacaggt 16140
caatgccgcc gagagtaaag ccacattttg cgtacaaatt gcaggcaggt acattgttcg 16200
tttgtgtctc taatcgtatg ccaaggagct gtctgcttag tgcccacttt ttcgcaaatt 16260
cgatgagact gtgcgcgact cctttgcctc ggtgcgtgtg cgacacaaca atgtgttcga 16320
tagaggctag atcgttccat gttgagttga gttcaatctt cccgacaagc tcttggtcga 16380
tgaatgcgcc atagcaagca gagtcttcat cagagtcatc atccgagatg taatccttcc 16440
ggtaggggct cacacttctg gtagatagtt caaagccttg gtcggatagg tgcacatcga 16500
acacttcacg aacaatgaaa tggttctcag catccaatgt ttccgccacc tgctcaggga 16560
tcaccgaaat cttcatatga cgcctaacgc ctggcacagc ggatcgcaaa cctggcgcgg 16620
cttttggcac aaaaggcgtg acaggtttgc gaatccgttg ctgccacttg tttaatagac 16680
tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg 16740
tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg 16800
gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact 16860
atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa 16920
ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt 16980
aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag 17040
ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgatatcct 17100
ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt 17160
tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 17220
cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct 17280
gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 17340
gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 17400
tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 17460
ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg 17520
gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 17580
ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 17640
tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 17700
ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct 17760
gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga 17820
acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt 17880
ctccttacgc atctgtgcgg tatttcacac cgcatatgaa gatcggcggc aatagcttct 17940
tagcgccatc ccggctgaga aagcccagta aggaaacaac tgtaggttcg agtcgcgaga 18000
tcccccggaa ccaaaggaag taggttaaac ccgctccgat caggccgagc cacgccaggc 18060
cgagaacatt ggttcctgta ggcatcggga ttggcggatc aaacactaaa gctactggaa 18120
cgagcagaag tcctccggcc gccagttgcc aggcggtaaa ggtgagcaga ggcacgggag 18180
gttgccactt gcgggtcagc acggttccga acgccatgga aaccgccccc gccaggcccg 18240
ctgcgacgcc gacaggatct agcgctgcgt ttggtgtcaa caccaacagc gccacgcccg 18300
cagttccgca aatagccccc aggaccgcca tcaatcgtat cgggctacct agcagagcgg 18360
cagagatgaa cacgaccatc agcggctgca cagcgcctac cgtcgccgcg accccgcccg 18420
gcaggcggta gaccgaaata aacaacaagc tccagaatag cgaaatatta agtgcgccga 18480
ggatgaagat gcgcatccac cagattcccg ttggaatctg tcggacgatc atcacgagca 18540
ataaacccgc cggcaacgcc cgcagcagca taccggcgac ccctcggcct cgctgttcgg 18600
gctccacgaa aacgccggac agatgcgcct tgtgagcgtc cttggggccg tcctcctgtt 18660
tgaagaccga cagcccaatg atctcgccgt cgatgtaggc gccgaatgcc acggcatctc 18720
gcaaccgttc agcgaacgcc tccatgggct ttttctcctc gtgctcgtaa acggacccga 18780
acatctctgg agctttcttc agggccgaca atcggatctc gcggaaatcc tgcacgtcgg 18840
ccgctccaag ccgtcgaatc tgagccttaa tcacaattgt caattttaat cctctgttta 18900
tcggcagttc gtagagcgcg ccgtgcgtcc cgagcgatac tgagcgaagc aagtgcgtcg 18960
agcagtgccc gcttgttcct gaaatgccag taaagcgctg gctgctgaac ccccagccgg 19020
aactgacccc acaaggccct agcgtttgca atgcaccagg tcatcattga cccaggcgtg 19080
ttccaccagg ccgctgcctc gcaactcttc gcaggcttcg ccgacctgct cgcgccactt 19140
cttcacgcgg gtggaatccg atccgcacat gaggcggaag gtttccagct tgagcgggta 19200
cggctcccgg tgcgagctga aatagtcgaa catccgtcgg gccgtcggcg acagcttgcg 19260
gtacttctcc catatgaatt tcgtgtagtg gtcgccagca aacagcacga cgatttcctc 19320
gtcgatcagg acctggcaac gggacgtttt cttgccacgg tccaggacgc ggaagcggtg 19380
cagcagcgac accgattcca ggtgcccaac gcggtcggac gtgaagccca tcgccgtcgc 19440
ctgtaggcgc gacaggcatt cctcggcctt cgtgtaatac cggccattga tcgaccagcc 19500
caggtcctgg caaagctcgt agaacgtgaa ggtgatcggc tcgccgatag gggtgcgctt 19560
cgcgtactcc aacacctgct gccacaccag ttcgtcatcg tcggcccgca gctcgacgcc 19620
ggtgtaggtg atcttcacgt ccttgttgac gtggaaaatg accttgtttt gcagcgcctc 19680
gcgcgggatt ttcttgttgc gcgtggtgaa cagggcagag cgggccgtgt cgtttggcat 19740
cgctcgcatc gtgtccggcc acggcgcaat atcgaacaag gaaagctgca tttccttgat 19800
ctgctgcttc gtgtgtttca gcaacgcggc ctgcttggcc tcgctgacct gttttgccag 19860
gtcctcgccg gcggtttttc gcttcttggt cgtcatagtt cctcgcgtgt cgatggtcat 19920
cgacttcgcc aaacctgccg cctcctgttc gagacgacgc gaacgctcca cggcggccga 19980
tggcgcgggc agggcagggg gagccagttg cacgctgtcg cgctcgatct tggccgtagc 20040
ttgctggacc atcgagccga cggactggaa ggtttcgcgg ggcgcacgca tgacggtgcg 20100
gcttgcgatg gtttcggcat cctcggcgga aaaccccgcg tcgatcagtt cttgcctgta 20160
tgccttccgg tcaaacgtcc gattcattca ccctccttgc gggattgccc cgactcacgc 20220
cggggcaatg tgcccttatt cctgatttga cccgcctggt gccttggtgt ccagataatc 20280
caccttatcg gcaatgaagt cggtcccgta gaccgtctgg ccgtccttct cgtacttggt 20340
attccgaatc ttgccctgca cgaataccag ctccgcgaag tcgctcttct tgatggagcg 20400
catggggacg tgcttggcaa tcacgcgcac cccccggccg ttttagcggc taaaaaagtc 20460
atggctctgc cctcgggcgg accacgccca tcatgacctt gccaagctcg tcctgcttct 20520
cttcgatctt cgccagcagg gcgaggatcg tggcatcacc gaaccgcgcc gtgcgcgggt 20580
cgtcggtgag ccagagtttc agcaggccgc ccaggcggcc caggtcgcca ttgatgcggg 20640
ccagctcgcg gacgtgctca tagtccacga cgcccgtgat tttgtagccc tggccgacgg 20700
ccagcaggta ggccgacagg ctcatgccgg ccgccgccgc cttttcctca atcgctcttc 20760
gttcgtctgg aaggcagtac accttgatag gtgggctgcc cttcctggtt gggtaatgac 20820
tccaacttat tgatagtgtt ttatgttcag ataatgcccg atgactttgt catgcagctc 20880
caccgatttt gagaacgaca gcgacttccg tcccagccgt gccaggtgct gcctcagatt 20940
caggttatgc cgctcaattc gctgcgtata tcgcttgctg attacgtgca gctttccctt 21000
caggcgggat tcatacagcg gccagccatc cgtcatccat atcaccacgt caaagggtga 21060
cagcaggctc ataagacgcc ccagcgtcgc catagtgcgt tcaccgaata cgtgcgcaac 21120
aaccgtcttc cggagactgt catacgcgta aaacagccag cgctggcgcg atttagcccc 21180
gacatagccc cactgttcgt ccatttccgc gcagacgatg acgtcactgc ccggctgtat 21240
gcgcgaggtt accgactgcg gcctgagttt tttaagtgac gtaaaatcgt gttgaggcca 21300
acgcccataa tgcgggctgt tgcccggcat ccaacgccat tcatggccat atcaatgatt 21360
ttctggtgcg taccgggttg agaagcggtg taagtgaact gcagttgcca tgttttacgg 21420
cagtgagagc agagatagcg ctgatgtccg gcggtgcttt tgccgttacg caccaccccg 21480
tcagtagctg aacaggaggg acagctgata gaaacagaag ccactggagc acctcaaaaa 21540
caccatcata cactaaatca gtaagttggc agcatcaccc ctggttggct tggtttcatc 21600
agccatccgc ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg 21660
attcccgttg agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc 21720
gggtgggcct acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc 21780
tacacgaacc ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa 21840
aatcgctata atgaccccga agcagggtta tgcagcggaa aagatcc 21887
<210> 159
<211> 20056
<212> DNA
<213> 人工序列
<220>
<223> 转化构建体
<400> 159
gtcgacgatc gtcaacgttc acttctaaag aaatagcgcc actcagcttc ctcagcggct 60
ttatccagcg atttcctatt atgtcggcat agttctcaag atcgacagcc tgtcacggtt 120
aagcgagaaa tgaataagaa ggctgataat tcggatctct gcgagggaga tgatatttga 180
tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc 240
cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca 300
aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg 360
tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca 420
ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt 480
ttttaatgta ctgaattcca atccccaaaa atctgagctt aacagcacag ttgctcctct 540
cagagcagaa tcgggtattc aacaccctca tatcaactac tacgttgtgt ataacggtcc 600
acatgccggt atatacgatg actggggttg tacaaaggcg gcaacaaacg gcgttcccgg 660
agttgcacac aagaaatttg ccactattac agaggcaaga gcagcagctg acgcgtacac 720
aacaagtcag caaacagaca ggttgaactt catccccaaa ggagaagctc aactcaagcc 780
caagagcttt gctaaggccc taacaagccc accaaagcaa aaagcccact ggctcacgct 840
aggaaccaaa aggcccagca gtgatccagc cccaaaagag atctcctttg ccccggagat 900
tacaatggac gatttcctct atctttacga tctaggaagg aagttcgaag gtgaaggtga 960
cgacactatg ttcaccactg ataatgagaa ggttagcctc ttcaatttca gaaagaatgc 1020
tgacccacag atggttagag aggcctacgc agcaggtctc atcaagacga tctacccgag 1080
taacaatctc caggagatca aataccttcc caagaaggtt aaagatgcag tcaaaagatt 1140
caggactaat tgcatcaaga acacagagaa agacatattt ctcaagatca gaagtactat 1200
tccagtatgg acgattcaag gcttgcttca taaaccaagg caagtaatag agattggagt 1260
ctctaaaaag gtagttccta ctgaatctaa ggccatgcat ggagtctaag attcaaatcg 1320
aggatctaac agaactcgcc gtgaagactg gcgaacagtt catacagagt cttttacgac 1380
tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca cgacactctg gtctactcca 1440
aaaatgtcaa agatacagtc tcagaagacc aaagggctat tgagactttt caacaaagga 1500
taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc atcgaaagga 1560
cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggctatca 1620
ttcaagatct ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg 1680
tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gacatctcca 1740
ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc tctatataag 1800
gaagttcatt tcatttggag aggacacgct cgagtataag agctctattt ttacaacaat 1860
taccaacaac aacaaacaac aaacaacatt acaattacta tttacaatta ccatgagccc 1920
agaacgacgc ccggccgaca tccgccgtgc caccgaggcg gacatgccgg cggtctgcac 1980
catcgtcaac cactacatcg agacaagcac ggtcaacttc cgtaccgagc cgcaggaacc 2040
gcaggagtgg acggacgacc tcgtccgtct gcgggagcgc tatccctggc tcgtcgccga 2100
ggtggacggc gaggtcgccg gcatcgccta cgcgggcccc tggaaggcac gcaacgccta 2160
cgactggacg gccgagtcaa ccgtgtacgt ctccccccgc caccagcgga cgggactggg 2220
ctccacgctc tacacccacc tgctgaagtc cctggaggca cagggcttca agagcgtggt 2280
cgctgtcatc gggctgccca acgacccgag cgtgcgcatg cacgaggcgc tcggatatgc 2340
cccccgcggc atgctgcggg cggccggctt caagcacggg aactggcatg acgtgggttt 2400
ctggcagctg gacttcagcc tgccggtacc gccccgtccg gtcctgcccg tcaccgagat 2460
ttgatgaccc gggggatcct agagtcctgc tttaatgaga tatgcgagac gcctatgatc 2520
gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct 2580
cagatcctta ccgccggttt cggttcattc taatgaatat atcacccgtt actatcgtat 2640
ttttatgaat aatattctcc gttcaattta ctgattgtac cctactactt atatgtacaa 2700
tattaaaatg aaaacaatat attgtgctga ataggtttat agcgacatct atgatagagc 2760
gccacaataa caaacaattg cgttttatta ttacaaatcc aattttaaaa aaagcggcag 2820
aaccggtcaa acctaaaaga ctgattacat aaatcttatt caaatttcaa aagtgcccca 2880
ggggctagta tctacgacac accgagcggc gaactaataa cgctcactga agggaactcc 2940
ggttccccgc cggcgcgcat gggtgagatt ccttgaagtt gagtattggc cgtccgctct 3000
accgaaagtt acgggcacca ttcaacccgg tccagcacgg cggccgggta accgacttgc 3060
tgccccgaga attatgcagc atttttttgg tgtatgtggg ccccaaatga agtgcaggtc 3120
aaaccttgac agtgacgaca aatcgttggg cgggtccagg gcgaattttg cgacaacatg 3180
tcgaggctca gcaggacctg caggcatgca agctctagag cggccgcttt aagagtgttg 3240
aggatgtgct tacttaaatt tcaaaagggc tatttaggga taatatttgg tgaagcatgg 3300
ctggtggact gaccataggt acccactgaa taaacatatc agcccaaagg cagagggtat 3360
ccctccctgt aatacgtact agtattcgag gagcgtgcta aagtagtatc ctctcttgat 3420
gctcctcgtc tccctccact gagtactccc ttcctctcct cattctcatc tcagtcctct 3480
cctcctccct cgaataagat agatccgccc ttgttgctga tctcatctcg cctcctattg 3540
gcctcgcccc tgccaactga ctgctctatt ggtgaccgtg gactttttgg gtatatatat 3600
ttcctgtcga tttgtttcca cttaaagtag caatgcttgg atctaaaact tattacttct 3660
cctgcttcag attctgatcc atttgtgttg attgaactca gggtgggatc gtggggctct 3720
gtatctatct aggatggata ttccaagcaa tttactctct tgagtgctgt tggtcttcct 3780
ctgctcctcg tctccgcttc ctctcagtct cctccattgc gctgcctgga acagatcgat 3840
ccgccttgtt gctgatctca tccatcctcc gtggagattt cgggtggcag tttccagtga 3900
ctattcagag caccatacta gtggcctggt aaaaatttcc ctcccatctt tcttgctatt 3960
ctcttcttat taatctgctg atttgcttga tggtaagttc ttacttattc ttccagatcg 4020
agtgaagcga catccgccgc cgtcttgtcc cctacgacgc cgctgtgttc tgctctgtcc 4080
tcctctgccg gtgaagcatc aacggtgacg gccatccggt gttgacgccg cggctgtgtt 4140
ctcctctgcc gtgaagtatc aaaggtgggt ttggatggat atcacagttt ggattggcaa 4200
ctctgttcta tagctcgttg aaattaaagc ttcacacagt tttgattgga ttacagctcc 4260
ccatattcat ctattttgca ccatgcatcg aatccactga cctccacacg ctcacggatc 4320
tggcctctgg tgcccacacg ctcacggatc cgacagagtg ctcccttcct atcatctcag 4380
tcctcatctc ctggtcgatc cggcttcttg tttggtgagc tcgtccaacc tccgagctcg 4440
cccctgacac ctgtggagtg gaggtatatt tctttccagc tgaagtacgc ttggatctaa 4500
aaacttacgt ctcctgcttc actgcttgct tgggagtctg atcgatttgt gttggttgaa 4560
tcatttgggg tggcagtgga gcagctagca agaatcatgg agtttccaat cattattcag 4620
agcaccacgc atggaatcca ctgacctggt aactttgtct catctttctt gctactgaaa 4680
tcttccacat ccccaccttc cttgttcatc tgctgatttc ccagattata cttccagatt 4740
gagtgaagcg ccgtcttgtc ccctcacgga tctggcctct ggtgcccagc cgccgctttc 4800
gcttcgttgt ccgacgaggc caccgccgct gtgttctgct ctgccgtgaa gcacaaaaag 4860
gtggggtctt cctctgctct ttctccactg acggcgatcc gaccttgaca cccgtggccg 4920
tggcagtggg gtcttcctct gctctgtttt tttaccagtt attggatcta aaaagggatc 4980
taaaagttat tacttctcct gcttgagatt ctgaccgatt tgtgttgatt gaatccaggg 5040
tggggtcgtg gggctctgta tctatctagt acggagtacc actgtattcc aagcaattta 5100
ctctcttgac tgctgttgga cttcctctgt ccccgcttcc attgtgctgc cttgaacaga 5160
tccatccacc ttgttgctaa tctcatccaa cctccgggta tgtttcttgt cgatttgttt 5220
ccagtaaagt agcaatgctt gggtctaaaa agtcattgct tctcctgctt gacgagtctc 5280
attgatttgc ccagtcatta ttcagagcac catgcatcca aggcgctgac ccggctataa 5340
gtttttatta cagggccgcc gtgttgtccc catcgagcaa agcgtttaat ccactgaccc 5400
gcttcgttgt ccgcacagcg tgaccatccg acgaagacgc cgccgttgtg ttctgctctc 5460
ctctgaagca tcaaaaggtg ggcttggtcc agatccccac attcatcgat ttggcttgcc 5520
aactcagttc caccagagct tggattgtgt tacagatccc taaattcatc gatttgcagg 5580
tttcagctga ttgatcaaga gagctctcaa tgatggcgga gtttgtagtc aggccactgg 5640
tatccacgtt gatgaacacg gcatccagct acctgctgga ccagtacaag gtgatggatg 5700
gcatgaagga gcagcgcgaa accctcaagc gccagctgcc agccatcttg gacatcatcc 5760
aagatgcgga gaagaaagga gcttccctac ctggagtgcg cgcttggctc gaagcactca 5820
agaaggtggc ctatgaggcg aacgatgtct ttgatgagtt caagtacgag gccctccggc 5880
gtgacgccaa gaagaaggga cactacaaga agcttggctt tgacatcgta agcctcttcc 5940
ctgctcacaa ccccattgta tttcgttaca ggatgggcaa gaagttgtgc aggattgtgc 6000
gcaaaattga gggccttgtc agagagatga atgacttcgg attcaaccaa actcagcaag 6060
caccaccatc caagcagtgg cgcaacacag attccataat aattgactct gagaaggata 6120
ttgtcagcag gtccagaaac gaagagaaga agaagatcgt tgatatattg attgatcaag 6180
ctggcgatag ggacctcatc gtccttccca ttgttggaat gggcgggctg ggcaagacca 6240
cctttgctca gcttgtctac aatgacccta taatcaagga gcatttcaag ctccagaggt 6300
ggtgttgtgt gtcagatgat tttgatgttg tgaagattgc aaacaacatc tgtgagacta 6360
atgagatcca tcgtgaaaag gcattgcaga accttcagaa agaagtaagt gggaagagat 6420
acctcattgt gttggatgat gtatggaatg aagatgctga taagtgggaa aaactgaaaa 6480
cctgcctgaa gcacggtggg aaggggagtg caatattgac gaccactcgc aatgtgcaag 6540
tagcacgaat tatgaagatg tgtattgctg atagccataa tctcagaaat ctagataaag 6600
tatttctgaa ggaaatattt gagaatagag cattctgttt gcaaaagcca aaggctgctg 6660
agctaagcga tgtggttgat aagattatgg acagatgtgg aggctctcct ttagctgcca 6720
aagcctttgg gtctatgttg agtaacaaga ctagcatgaa agaatggaca gacatactag 6780
ccagaagcaa cacttgcaat gagggcacaa aaacttttct tgtactcaag ctcagctatg 6840
atgacttgcc atcacacttg aaacaatgct ttgctttttg tgctgtattt cccaaagatt 6900
atgagattgg tgttgaaaca ttaattcagc tatggatggc acatgatttc ataccactga 6960
aagaaggtga taatcttgag aaggtaggta gagaaatttt tgatgagtta acttggaggt 7020
cattctttca agatgtcaag cgaatccctc gaagagagtg gtggggggag ctccgtccta 7080
gaacaatatg caagatacat gatcttatgc atgacattgc cttgtctgtt atgggaaaag 7140
attgtcttac tatagttgat aggcctaatg aaaaggagtt actgtccact ggtcctacac 7200
gttacttgtt ctcatcatat gagtatatcg gaactctgtt ggatgattat ctgaagaaac 7260
actctcccgc tctccagaca ctgctgtatc cgtatcccta caccagtgac tcagcaccac 7320
acttgtcgaa gtgcaattat ctgcgagcac tgcaactctt cagtttgaga aaacttccac 7380
tctggccaag gcacttacag cacctgaggt atcttgatct ctcaaataac atgttgatcg 7440
aagagcttcc caaggaaata agcatactgt ataatctaca gactcttaac ctttgtaact 7500
gcagaaggct tgatcaactt ccagaggata tgaagtatat ggaaaatctc cgtcacctct 7560
atacaaatgg atgctcatca ttgaagtgca tgcctccagg cctcgggcaa ctcacttctc 7620
tacagactct aacatatttt gtggtgagct ctagtcctgg ttgcagtact attagagaac 7680
tacaggactt gaatcttggt ggtgagttag agctatctcg tctgcagttt gcaactgaag 7740
tagatgctaa agcatgcagc cttggaaata aagagaaact tacacattta tctcttaaat 7800
ggggtgatga cagcagtgat gaattgggcc accacaggaa tgtgctcgat gctcttaaac 7860
ctcatgctgt cctggagttt ctaagaatac gctcctatag aggtactggc ttccccgcat 7920
gggtggtaag tattaatttt ctgcagcatt tgaccgagct ccagctagat ggttgtacaa 7980
tgtgtgagga atttccccaa ttcggtcaat ttaagtccct tgaagttctt gttttgaaaa 8040
ggttgaacaa acttcaaagc ctatgcaatc acagttcatc tgcaatattt ccagcattaa 8100
aagttctcag attaaaaaaa ttggagattt ttgagagatg ggtggcaaca gaaggagaag 8160
agttagcatt tcctcaactt gagaatgtta aaattaagga ctgcccaaag ctagcaattc 8220
tgcctgaagc accaaaactc aagtttatag ctctaaaaga agaaaaggca cagctatcct 8280
tatcaatatt taagtccagg tatatggctt gcttgtctgg ggttggcctg tccgtccgtg 8340
atacagaagc agcaccaaga acagagcttg atcaggattg tgaagtatct ctttcgaacc 8400
tgctgttgga tggttgcaac tttttgttct gctcaactcc attgcagcca actgttgggg 8460
tctggaaatg gtttggtcag cttgtgcatt tggaaataaa atcttgtgat atgctcatct 8520
actggcctga agaagagttc cgttgcttgg tatcattgaa tagtttatcc attaattcct 8580
gcagcaagct agtaggacac acccagggga aaggatgccg tacccgtact caagtaaggg 8640
atcaactcct gccaaatctt aaaaatttaa gaatacatca ttgtggaagt ttgacagagc 8700
tttttgttct gccgccatct ctcacaagta ttgatatgct ggattgcaat agtattgagt 8760
ccatactggg gcaggatgat acagagttgg agagtatact acactttgat acagcatcat 8820
catcggaaca cttcaatgac ctcacgtcca catgtttgct agagcagtca ctttcaccca 8880
gaattaatcc tttgccatgt cttgattatt tgcgtatagt tagctgtaag aagcttcgtt 8940
tcgtgccagt gcagttagat gcactccaat ttttgtgtat ttctgattgc aatgggctgg 9000
agtcgctgga ttgtttggga gatctaccgt cactggaata tctatacctt atgggctgca 9060
aacatctggc atctgtacca ggaagtcttg ggagttactc ggcccttcag aagctccgca 9120
ttgaatactg cccagcttta aatatgaagc cacttcatgg acatctccaa cagcggcttg 9180
atagccttga attgaaggat ctatctaagg ctggttcaag ccatcctaat gaaggtacct 9240
ttcaatttca tttaccccat tttgtatctc tttttccatc tgtatagttt acagatgtac 9300
atattgcagc ctgtagtttc aagtttatcc aactccttgt attttcacat ttggtatgta 9360
gtgaccgcgt acacagtttc tctgatatgg ctattccaat atcatttatt tgtataccgt 9420
aagtttgact tggattgacg tcgtcatgtg tggccgatct gtattttcta tcattttggt 9480
aaaagttaat acaccatgga agtatgctct cttgtgtagt gaccgtacac agatcattct 9540
tttatgtcta tcccactatc atttatttgt aaagtttgac ttgctattaa tgttcacaga 9600
gatattctga cattgttttc catgtacagg gcctaaacta tgggaaccaa aatcctggaa 9660
atatatgatt ccttcactgc gcaagcgtga gtctgaatag gcttctgacg ttgcttcagg 9720
tgtgctctca atctatacat atttactcat catattattc tccacctttt tttccatctc 9780
tagagctcag cttgtcattg cattgttcaa ttgtgcttct ctagcagcta cggaaattct 9840
gaacaggcaa aggtaatttt acagaagttt ctttggcata agtatctgag aaaaatgcac 9900
ttggcacaat ttaccttttc gcagtagaaa agagtcagtg gatcatacct tcttaattgt 9960
tcgattgctc gtaatacttg ggggcactct atgattgctg tgtgctttta gttttgatat 10020
agttcctgtt agcatggaca gactgtgtga ttggttcatg aatttcaggg tggttctaat 10080
gaggaaatga tgcttgcttc agaaatagtt tccatttgta cctaagctgt gtagttggta 10140
aagttgcatt ttggatgttc tgttggtata gcttacagaa gaaagacttg cgttgtgcag 10200
gtaccagggg cgcaggttac tgctagtggc gtctgagatg tttcataaac gttgggtggg 10260
caccggtcat gcaaaaaatt gaaggataaa aaggttccga gaggttgtgt gatgtgctcc 10320
ttttaaatgg cagtcgcttg cataaagaga tgttgcttgg ttttttagta aagcgttgta 10380
gtaagttgct ctgtttcggc tgagagccat cgttgttgct gtctgttaca tcgaatggct 10440
ggctcatgtc atgttgatgt tttttttatc tgaaccggac aaacctgagg gtgtagtatc 10500
atgtttgtaa tgactgaaat tctgggctca cccgagctcc ttaattcgta aacaactgcg 10560
actcattgtt ggattcctta cttcagaaaa tatttacttg ctctggctca tacagctctg 10620
catgttttgt agtggtttgt cgctgtttag gtgctgcttc agactcctgg tacctttggc 10680
cgctgctgct actgcatgag ctttgtatgg cattgttagt atagtttggt gtcattgggt 10740
tttcgcccca tggtgagtgt ccattgcgcg cactcgcagc ggtttcctgg cgataagttg 10800
aacttgatct ttctctttcc cacctcctaa acgtctattc agacttgtgt atatttgcat 10860
tacggggatt acccgagttc aaaaataaat attatttact agttctgcca atgtgtttcc 10920
gttacataca aataaaaggg tgcttccagc tacaagtata tttttgagaa gggccattgc 10980
aaaacgaaat gaagcccaaa ttctaccaac atgtttctgt cacatataca ttaaaaaaac 11040
ggtgtaaatg tcccgtcaaa aaagggtgta tgtaggaaaa ggagttcgac gccgccagct 11100
ccctcctaac gctcagattg tcttcgcggc cgccaccgcg gctgagtggc tccttcaagc 11160
ttgcggatcc gggcggaaat aggtaaagaa gttgcggata aggtaattgc cattgcagat 11220
tatttggatt gagagtgaat atgagactct aattggatac cgaggggaat ttatggaacg 11280
tcagtggagc atttttgaca agaaatattt gctagctgat agtgacctta ggcgactttt 11340
gaacgcgcaa taatggtttc tgacgtatgt gcttagctca ttaaactcca gaaacccgcg 11400
gctgagtggc tccttcaacg ttgcggttct gtcagttcca aacgtaaaac ggcttgtccc 11460
gcgtcatcgg cgggggtcat aacgtgactc ccttaattct ccgctcatga tcagattgtc 11520
gtttcccgcc ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta 11580
agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc 11640
gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggagtg cttggcattc 11700
cgtgcgataa tgacttctgt tcaaccaccc aaacgtcgga aagcctgacg acggagcagc 11760
attccaaaaa gatcccttgg ctcgtctggg tcggctagaa ggtcgagtgg gctgctgtgg 11820
cttgatccct caacgcggtc gcggacgtag cgcagcgccg aaaaatcctc gatcgcaaat 11880
ccgacgctgt cgaaaagcgt gatctgcttg tcgctctttc ggccgacgtc ctggccagtc 11940
atcacgcgcc aaagttccgt cacaggatga tctggcgcga gttgctggat ctcgccttca 12000
atccgggtct gtggcgggaa ctccacgaaa atatccgaac gcagcaagat cgtcgacccc 12060
tttccgacgc tcaccgggct ggttgccctc gccgctgggc tggcggccgt ctatggccct 12120
gcaaacgcgc cagaaacgcc gtcgaagccg tgtgcgagac accgcggccg gccgccggcg 12180
ttgtggatac ctcgcggaaa acttggccct cactgacaga tgaggggcgg acgttgacac 12240
ttgaggggcc gactcacccg gcgcggcgtt gacagatgag gggcaggctc gatttcggcc 12300
ggcgacgtgg agctggccag cctcgcaaat cggcgaaaac gcctgatttt acgcgagttt 12360
cccacagatg atgtggacaa gcctggggat aagtgccctg cggtattgac acttgagggg 12420
cgcgactact gacagatgag gggcgcgatc cttgacactt gaggggcaga gtgctgacag 12480
atgaggggcg cacctattga catttgaggg gctgtccaca ggcagaaaat ccagcatttg 12540
caagggtttc cgcccgtttt tcggccaccg ctaacctgtc ttttaacctg cttttaaacc 12600
aatatttata aaccttgttt ttaaccaggg ctgcgccctg gcgcgtgacc gcgcacgccg 12660
aaggggggtg cccccccttc tcgaaccctc ccggcccgct aacgcgggcc tcccatcccc 12720
ccaggggctg cgcccctcgg ccgcgaacgg cctcacccca aaaatggcag ccaagctcct 12780
aacattttat tagagagcag gctagttgct tagatacatg atcttcaggc cgttatctgt 12840
cagggcaagc gaaaattggc catttatgac gaccaatgcc ccgcagaagc tcccatcttt 12900
gccgccatag acgccgcgcc ccccttttgg ggtgtagaac atccttttgc cagatgtgga 12960
aaagaagttc gttgtcccat tgttggcaat gacgtagtag ccggcgaaag tgcgagaccc 13020
atttgcgcta tatataagcc tacgatttcc gttgcgacta ttgtcgtaat tggatgaact 13080
attatcgtag ttgctctcag agttgtcgta atttgatgga ctattgtcgt aattgcttat 13140
ggagttgtcg tagttgcttg gagaaatgtc gtagttggat ggggagtagt catagggaag 13200
acgagcttca tccactaaaa caattggcag gtcagcaagt gcctgccccg atgccatcgc 13260
aagtacgagg cttagaacca ccttcaacag atcgcgcata gtcttcccca gctctctaac 13320
gcttgagtta agccgcgccg cgaagcggcg tcggcttgaa cgaattgtta gacattattt 13380
gccgactacc ttggtgatct cgcctttcac gtagtgaaca aattcttcca actgatctgc 13440
gcgcgaggcc aagcgatctt cttgtccaag ataagcctgc ctagcttcaa gtatgacggg 13500
ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat 13560
tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc 13620
gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag 13680
atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct 13740
atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa 13800
gatacctgca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg 13860
ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat 13920
ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt 13980
tgtttcatca agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag 14040
gccgccatcc actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg 14100
ctcgatgacg ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttccct 14160
catgatgttt aactcctgaa ttaagccgcg ccgcgaagcg gtgtcggctt gaatgaattg 14220
ttaggcgtca tcctgtgctc ccgagaacca gtaccagtac atcgctgttt cgttcgagac 14280
ttgaggtcta gttttatacg tgaacaggtc aatgccgccg agagtaaagc cacattttgc 14340
gtacaaattg caggcaggta cattgttcgt ttgtgtctct aatcgtatgc caaggagctg 14400
tctgcttagt gcccactttt tcgcaaattc gatgagactg tgcgcgactc ctttgcctcg 14460
gtgcgtgtgc gacacaacaa tgtgttcgat agaggctaga tcgttccatg ttgagttgag 14520
ttcaatcttc ccgacaagct cttggtcgat gaatgcgcca tagcaagcag agtcttcatc 14580
agagtcatca tccgagatgt aatccttccg gtaggggctc acacttctgg tagatagttc 14640
aaagccttgg tcggataggt gcacatcgaa cacttcacga acaatgaaat ggttctcagc 14700
atccaatgtt tccgccacct gctcagggat caccgaaatc ttcatatgac gcctaacgcc 14760
tggcacagcg gatcgcaaac ctggcgcggc ttttggcaca aaaggcgtga caggtttgcg 14820
aatccgttgc tgccacttgt ttaatagact ggatggaggc ggataaagtt gcaggaccac 14880
ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 14940
gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 15000
ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 15060
taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 15120
agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 15180
atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 15240
aaaagatcaa aggatcttct tgatatcctt tttttctgcg cgtaatctgc tgcttgcaaa 15300
caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 15360
ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 15420
cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 15480
tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 15540
gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 15600
ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa 15660
gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 15720
caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 15780
ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 15840
tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 15900
ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 15960
agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 16020
aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc 16080
gcatatgaag atcggcggca atagcttctt agcgccatcc cggctgagaa agcccagtaa 16140
ggaaacaact gtaggttcga gtcgcgagat cccccggaac caaaggaagt aggttaaacc 16200
cgctccgatc aggccgagcc acgccaggcc gagaacattg gttcctgtag gcatcgggat 16260
tggcggatca aacactaaag ctactggaac gagcagaagt cctccggccg ccagttgcca 16320
ggcggtaaag gtgagcagag gcacgggagg ttgccacttg cgggtcagca cggttccgaa 16380
cgccatggaa accgcccccg ccaggcccgc tgcgacgccg acaggatcta gcgctgcgtt 16440
tggtgtcaac accaacagcg ccacgcccgc agttccgcaa atagccccca ggaccgccat 16500
caatcgtatc gggctaccta gcagagcggc agagatgaac acgaccatca gcggctgcac 16560
agcgcctacc gtcgccgcga ccccgcccgg caggcggtag accgaaataa acaacaagct 16620
ccagaatagc gaaatattaa gtgcgccgag gatgaagatg cgcatccacc agattcccgt 16680
tggaatctgt cggacgatca tcacgagcaa taaacccgcc ggcaacgccc gcagcagcat 16740
accggcgacc cctcggcctc gctgttcggg ctccacgaaa acgccggaca gatgcgcctt 16800
gtgagcgtcc ttggggccgt cctcctgttt gaagaccgac agcccaatga tctcgccgtc 16860
gatgtaggcg ccgaatgcca cggcatctcg caaccgttca gcgaacgcct ccatgggctt 16920
tttctcctcg tgctcgtaaa cggacccgaa catctctgga gctttcttca gggccgacaa 16980
tcggatctcg cggaaatcct gcacgtcggc cgctccaagc cgtcgaatct gagccttaat 17040
cacaattgtc aattttaatc ctctgtttat cggcagttcg tagagcgcgc cgtgcgtccc 17100
gagcgatact gagcgaagca agtgcgtcga gcagtgcccg cttgttcctg aaatgccagt 17160
aaagcgctgg ctgctgaacc cccagccgga actgacccca caaggcccta gcgtttgcaa 17220
tgcaccaggt catcattgac ccaggcgtgt tccaccaggc cgctgcctcg caactcttcg 17280
caggcttcgc cgacctgctc gcgccacttc ttcacgcggg tggaatccga tccgcacatg 17340
aggcggaagg tttccagctt gagcgggtac ggctcccggt gcgagctgaa atagtcgaac 17400
atccgtcggg ccgtcggcga cagcttgcgg tacttctccc atatgaattt cgtgtagtgg 17460
tcgccagcaa acagcacgac gatttcctcg tcgatcagga cctggcaacg ggacgttttc 17520
ttgccacggt ccaggacgcg gaagcggtgc agcagcgaca ccgattccag gtgcccaacg 17580
cggtcggacg tgaagcccat cgccgtcgcc tgtaggcgcg acaggcattc ctcggccttc 17640
gtgtaatacc ggccattgat cgaccagccc aggtcctggc aaagctcgta gaacgtgaag 17700
gtgatcggct cgccgatagg ggtgcgcttc gcgtactcca acacctgctg ccacaccagt 17760
tcgtcatcgt cggcccgcag ctcgacgccg gtgtaggtga tcttcacgtc cttgttgacg 17820
tggaaaatga ccttgttttg cagcgcctcg cgcgggattt tcttgttgcg cgtggtgaac 17880
agggcagagc gggccgtgtc gtttggcatc gctcgcatcg tgtccggcca cggcgcaata 17940
tcgaacaagg aaagctgcat ttccttgatc tgctgcttcg tgtgtttcag caacgcggcc 18000
tgcttggcct cgctgacctg ttttgccagg tcctcgccgg cggtttttcg cttcttggtc 18060
gtcatagttc ctcgcgtgtc gatggtcatc gacttcgcca aacctgccgc ctcctgttcg 18120
agacgacgcg aacgctccac ggcggccgat ggcgcgggca gggcaggggg agccagttgc 18180
acgctgtcgc gctcgatctt ggccgtagct tgctggacca tcgagccgac ggactggaag 18240
gtttcgcggg gcgcacgcat gacggtgcgg cttgcgatgg tttcggcatc ctcggcggaa 18300
aaccccgcgt cgatcagttc ttgcctgtat gccttccggt caaacgtccg attcattcac 18360
cctccttgcg ggattgcccc gactcacgcc ggggcaatgt gcccttattc ctgatttgac 18420
ccgcctggtg ccttggtgtc cagataatcc accttatcgg caatgaagtc ggtcccgtag 18480
accgtctggc cgtccttctc gtacttggta ttccgaatct tgccctgcac gaataccagc 18540
tccgcgaagt cgctcttctt gatggagcgc atggggacgt gcttggcaat cacgcgcacc 18600
ccccggccgt tttagcggct aaaaaagtca tggctctgcc ctcgggcgga ccacgcccat 18660
catgaccttg ccaagctcgt cctgcttctc ttcgatcttc gccagcaggg cgaggatcgt 18720
ggcatcaccg aaccgcgccg tgcgcgggtc gtcggtgagc cagagtttca gcaggccgcc 18780
caggcggccc aggtcgccat tgatgcgggc cagctcgcgg acgtgctcat agtccacgac 18840
gcccgtgatt ttgtagccct ggccgacggc cagcaggtag gccgacaggc tcatgccggc 18900
cgccgccgcc ttttcctcaa tcgctcttcg ttcgtctgga aggcagtaca ccttgatagg 18960
tgggctgccc ttcctggttg ggtaatgact ccaacttatt gatagtgttt tatgttcaga 19020
taatgcccga tgactttgtc atgcagctcc accgattttg agaacgacag cgacttccgt 19080
cccagccgtg ccaggtgctg cctcagattc aggttatgcc gctcaattcg ctgcgtatat 19140
cgcttgctga ttacgtgcag ctttcccttc aggcgggatt catacagcgg ccagccatcc 19200
gtcatccata tcaccacgtc aaagggtgac agcaggctca taagacgccc cagcgtcgcc 19260
atagtgcgtt caccgaatac gtgcgcaaca accgtcttcc ggagactgtc atacgcgtaa 19320
aacagccagc gctggcgcga tttagccccg acatagcccc actgttcgtc catttccgcg 19380
cagacgatga cgtcactgcc cggctgtatg cgcgaggtta ccgactgcgg cctgagtttt 19440
ttaagtgacg taaaatcgtg ttgaggccaa cgcccataat gcgggctgtt gcccggcatc 19500
caacgccatt catggccata tcaatgattt tctggtgcgt accgggttga gaagcggtgt 19560
aagtgaactg cagttgccat gttttacggc agtgagagca gagatagcgc tgatgtccgg 19620
cggtgctttt gccgttacgc accaccccgt cagtagctga acaggaggga cagctgatag 19680
aaacagaagc cactggagca cctcaaaaac accatcatac actaaatcag taagttggca 19740
gcatcacccc tggttggctt ggtttcatca gccatccgct tgccctcatc tgttacgccg 19800
gcggtagccg gccagcctcg cagagcagga ttcccgttga gcaccgccag gtgcgaataa 19860
gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat cctgcccggc 19920
tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa tcctgtatat 19980
cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa gcagggttat 20040
gcagcggaaa agatcc 20056
<210> 160
<211> 7815
<212> DNA
<213> 人工序列
<220>
<223> 转化构建体
<400> 160
gcgaacttta ctgtcaacgg acacaactac gacaaacgat actacctggg tgatagtatc 60
tatcctagtg gaccactatt gtcaagataa tacccaaccc tgtcggagag aagaggaaaa 120
gatttgccca tgagcaagag agtgctagga aggatgtcga gcgtgccttt ggtgttttgc 180
aatctcgatg gggcatcgtt cggtatgctg ctaatatctg gagcacgcag aaactgtggg 240
aggtgatgac tgcttgtgtg atcatgcata atatgatcgt agaagacgag cgaccggaac 300
gtctgtacga tcaagggttt cagtttcaga gtgagaatgt tgtgcctgag catggagtag 360
cagcaacgtt tgaacagttc atccaatttc atcaagacat gcgtgattgg gaaactcacg 420
tgcagccgca aaatgatttg gttgagtata tgtggggtca tgttggcaac caatagatat 480
atcttctttt attcgtttgc aaaactatga aacatttatt tttattttta tttgtatccg 540
gcttgtaaaa ctatactatt ttatttggta aactatgtta tttgacaata tgtttgaatg 600
caaatcaata taatattggg cggccagcca gccacgccga cacgtatagg tcgacgcgtt 660
gggcgcgctg ccgactcaaa tctaaaaaag ggcggacgcc gggcgggcgg gcgacccaaa 720
cgaacaaaaa gcgaatgaaa tcgccgtcca tttggatcgc tccatcggag ttgctctgtc 780
tggccgccaa atggggacgt gacggacacc gagtgaactc gtgcggtgtg tccgcttcac 840
atctaaattt aggtaaaaaa atgaatccgt atggacatgt tttgagaatg ggtcgttacg 900
ttgcgacaat atttttatcc acaacgacct aaacaaatgc gggcagatga aatgtatggc 960
tgcgttggag atgctgttaa cggagcaaag tcgttggtag tcggaccgag caaagtaatt 1020
gctgaggcag gtagtggacg agactgtaga aaggttccac gcttaatatc ttgagcagta 1080
cttgtagcac atgtgtccct gcaaagctgt ctccgtgctg tataccatgt gaattgcttt 1140
tctcaaaagt agtggagtac cctaccgtgt ttcattagtc ttgccggctt catagacaaa 1200
ggattctttt ttcccgtgcc atcgccatct gcaagcccaa atcaacacac accagagaga 1260
gagagagaga gagagagaga gagagagcaa caatggcgga agttctggtg agcgcatcca 1320
caggggcaat gggttccctc ctgcggaagt tgggagctat gttgacggat gaatacaagc 1380
tgctcaagaa cgtccgtggg gacatcaagt tcctcaagga cgagctggag gtcatgtgcg 1440
cgttcctcct caagatgtca gatgtggagg agcctgacga accaacaaag ctgcgtgtga 1500
cggcggtgcg ggagatgtcc tataagatag aggacaacat cgacaagttc atggtcctcg 1560
tggaacagga acacgggtcc tcctgttccg aggcggctca tggcgtcgcc aagctcatgg 1620
acaagtgcaa gaacttgctg ccggacatca aggcccgccg caggatcgcc aaggaggtca 1680
aagacatcaa gaaagaaatt aaggatgtca gcgacagatt ttcaaggtac cagatcttgt 1740
tctccttaca gtactattag tacctaccat cagatttgtg accgatttgt tgccactcaa 1800
ggtacaagat tgacgactcc tcctcctcta tgccggcaaa agacaaggtc gaccctcgac 1860
ttcgtgcagt ctacaaagac gccgcagagc tcgtcggaat tgatggacca aaggatgaac 1920
ttgtcaagtg gctgaatgag aaagaaggcc aatcactgaa atctgtctct attgttggat 1980
atggagggtt gggcaagacc acgctcgcca accagatccg ggtcaacctt ggagcgacct 2040
tcgactgcgg ggcttttgtc tcgatttcac gcaagcctga catgaaggcg atattaagat 2100
ctatattatc acaaatcacc aagaaagacg atgcttgctc tagattagat gatatacaac 2160
tcatcatcga caagattcga gaattcctcc aagacacaag gtatgtatgt gagatctgct 2220
acgaagtaac attgcttaat tagctcatgc ataatcggtt gcctagaatt ttgctaatga 2280
agattgcttt gtgtgattta ctttcgtcct caacttgtat gactgcgctt aaactttcaa 2340
gtcattgttt cttgcaactt atgtcgatct gttcactaat taagatatag ctaaaatgtg 2400
tttgccgatt tctgtcgcca cacattttct aatttgattg ccacaagttt gttaaacttg 2460
cggctatgtt ctgtttatta aataattgtg acatggcgtc agtcaaaaca ataaagtaca 2520
tgcaattccc tctcttttgt acaagataaa tatccgcacg cactacatga ctgcaaatat 2580
atctatcttc taaggaccca aagcacttgc atgtgcagac cattgttacc ggatatgcag 2640
tacattaggt ttgtgattag ccactttaag aacatagggt ttctagaggc tataaaccta 2700
cggttatcag tattataatc ttgaatggat atcaaacatc cgttgaagaa tctatgccca 2760
taacacaaaa agagaaaagg gaagaattaa tcatagtcag atcggaccag cctgactagc 2820
gtaacataat gacaacagta ggttgttgcg tctttcctgg tttcgtagca atttgcatga 2880
cgaattgagg cgcagagcca ccactcaata tgtggtccta ccaatcatga ggaaataact 2940
tgttaccaaa atgacaaaag aaaataggta gtccccatca ttgtttcaat atctatcctc 3000
ttgcgaacat gctattggca gtggcaaaac attgtacctt gccactggca acagctgcct 3060
ttactcggcc tgagcattca tatttttttc ctcttggttg ttaaatcttg ctatttttcg 3120
cttgccttct atggcaatct ttttttttct tatattgcca ccaaattgta tctaagagca 3180
actccaacac gccgacgcaa tttttgtcca tttagatcgg ccaagcggac ggggtgtctg 3240
gttttgcctt tgggtggtgc gcctacgcat tagtccgcgt gggcatttaa aaaacttaaa 3300
aagcttaaaa taattaaata taaataaaaa tgcatattaa agcccgtcaa agtccacata 3360
attaaacata atacattaat atattaataa aaaacagccg ccccagcccg cagccctagg 3420
cgtcttcgtc ggaggagtgg gtgaggtcga agaatgccgg cagctcagcc cacgggaagg 3480
cctgctggtg ggcagccagc accgcctgct ccgctgtctg cggcggcggc ggccctacct 3540
ggcaccgcgc acgcctcctc ctcgcgctcc tgccgctcct cctcctcatc gcacgcccaa 3600
cgaagttttg gcgctcctcc ccctcgggtt cccgctgcat gcggacctgg ccgtcgtacc 3660
gctcctcctc cagcgtgcgg tccttccacg ccacgaggta ggccgcctcc tcctccatca 3720
cgcacagcca aatgggcggc ttgctcatcc gcttgcagac gatgccctgc cattggtaaa 3780
cgccggtggg cgcgggtggt gctagcgctg gtgcaggcgg tgacgatgtg gctggtggtc 3840
ccggcaggta gacactatag ctggcggccg atagctgcag cgtgtcatcg aggccagacc 3900
actaggagtc ctcgcactaa gactcctcgg tgacgtgatg ggccccctcc tccaatgcct 3960
tgaggaacgc cgcctcctcc tcaacctact atttgaccac cggtggcggc ggtggagggc 4020
aggcttcgag ctggacggcg tcgcggaggc gcacatggtg ctcgatggtg aaccagactt 4080
ctcagttagg ggagtctggc ctcgatgggt ggagtcgcga cgctgctctg gcgtcaggag 4140
agcacgccgg gcccgaatct cgttggcgcg cgcacgctcg gactgtggca ccgccggcac 4200
tggaatgtgg tgggggtcga ggtgccagcc gtgggggaag ttcacgtccg gccacggcag 4260
tgagacgcgg tactcccggt ggtactacgc gtggtgcacc gggatgtact ggtggtcccg 4320
tggcgctgcc gccctcccat cccgcagcgc cgacaccaat gcgaacccct tcgctgccgg 4380
cctcaccggc ccttgcaacg agccggcctc gtggtcgtgc ttgccttgcc cttacctttg 4440
ccaaagaaac ccatgacggc tagggttttc tgtcaccggc gagggagcag atgtggacta 4500
gagtgggagg agaagtggat gaggcccgcc ccgccgcacc cttgcaatta aaaagggcgt 4560
gatccggacg tttctagtca ctgccatgcg ggcccgaggt aggtgggcgc cattgactac 4620
gtctgtggtg gtagttggct ggcagacacg ccagcccgag gcgcacaccg agcggacgcg 4680
caacacgtcc gtctcgtgtt cgtgtgaacg caaatcaggc acaaatttgg gctggaaatg 4740
cgtctacatg gatgccgaac ggacgtgatt tgggtttgtg tccgcacgtt gggccggcgt 4800
ttttgtgcct ttcgacccta atggacacac acggaccaaa tccgttggcg tgttagagtt 4860
gcttgtacct agtactacta tgtgtggagt tgttatcttt gagtttctcc cccatctgta 4920
gctgtgtatt attgtgagat gctctcagga tttcacttat tttagataat gacaactttc 4980
ttgatgctag acaattacat caaggtgtat tatatctagg atgcacacag atgtgagatt 5040
gtttcaggat tttaggttac ttggtaggtg ttactataaa tatctaatga gctctaatgt 5100
gtatgttcat gaaactgtgt tgcaggtact ttatcataat tgatgatata tgggagttag 5160
gaacatggga aactttgaaa tgtgcatttg tcaagaatac attgggcagc agaataatta 5220
tcacaacacg tatagttgat gttgccaaat cttgttctcc ttctagtgaa gatcttgtct 5280
atgagatgaa accactcagt gaggctgact cgaagaaatt gtttttcaag aggatatttg 5340
gctgtgaaga aagctgtcct gatagcttga aagaagctgc caatgatatt ttgaaaaaat 5400
gccgtggcct acctctggcc atcaatgcca tttctagcgt gttggtcaca acaagggaaa 5460
caaaagaaga gtgggatcgg gtgcgacatt caatacgctc ttcaaaggtc aaaagtgata 5520
taatagagac catgaattac atattgtctc ttagttattt tgatcttccc caccacctaa 5580
gaagctgctt attgtacctg gctctgtttc ctgaagacca attgattgga aggaagcggt 5640
tagtacgcag atggatttct gaaggcttta tccatggaga aagtggacaa gatctcatgg 5700
aattaggaga ggaatatttt caccagcttg tgaacagaag tctaatacag cccggcaaca 5760
tagggtatga cggcaaggca atgtattgcc gagtccatga caccatcctt gatttcctaa 5820
ttgacaaatc ctctgaagag aacatgtgta ctgtcctaaa gaaacaatgc aagcctaatg 5880
gcatagttcg tcggctctct ctaatgggga atgaggatga agaaatcgtg gagcaattgg 5940
atctgtcaca cgctcggtca atcactgctt ttggggatat caagctattg ccttcgcttg 6000
ggaggtcaaa atgcttgcgc gtgctagact tgcaagactg cgatcagttg gaaaatcatc 6060
atatcaagga tattgaaaga ctctatcagc taagatactt ggatatctct tctacaggaa 6120
tcacggagct tcccaggcaa attggggagt tgctgtatct agagacgcta gttgcctatg 6180
gattacgtga gcttcctgaa agcacaagtc ggctacaacg actggcacgt ttgtttgttt 6240
actctggttg taagcttcca ggtgggttgg gaaacttgat aaacttgcaa gagcttgact 6300
gcgttgatgc cttgcatttg aagcatgtgg aagagctcgg caaactaaca aatctgagaa 6360
agctgagtat taaattggac actggtggga ttgaaggcaa caaattagaa gagtccaagg 6420
aaaagctggt gtcctctctc tgtaaactgg acgaatgcgg cctcctttcc ctgagtattg 6480
attattatct gagagaaaag gatggagaag agccgttcct ccctgctttg ggttgtatcc 6540
aggaggtttt tgtatatgga caagatatca gccggattag cagatggctc gcgtcacttc 6600
ccaacctaca caggttgctt ttggacgatc cgaagataga gcagcaggat attgagatga 6660
ttggattgat acccaatctg atcgatttga cactgccgcc tctatacaaa acagacgacg 6720
ctgggcggct catcatcagg cgtgaaggat ttcaacagtt gcagaagttt gaggcttaca 6780
atactcgtat gggggtttta atgtttgaac cgggggctat gccgaggctc aaggagctta 6840
aactccataa cttcatcgaa aagcccaaat ctgctgcggt cgatttcgac tttggcatcc 6900
aacgcctctc cagccttgct cgcctcactg tcagcttatc ctgcggtggc tggacggtgg 6960
cagaagtaga ggctgcggag gatgctttca agagcatggc cgaggcgaac cccaaccgtc 7020
caatactgga gatgacaaga tataatacgc agcatatgtt acaagacgag caaataggta 7080
tgacaggctc aggtactatt aactagtttg tacttttgtc ttggtgagca gtagatcaaa 7140
agatgtgcat atgcttcaat cttttttccg gtaatctatt tcagctacaa cgccggccgt 7200
caagtcctag atgcctaaac cgagatgcgc ctttggttat ggagcccaag tcacgtactc 7260
ggattggatg ctgcagcaaa tgatacttct gtacatgtct gcaagtatca cgaattagtg 7320
gaatgactag gaataagtaa gacaagtatc acgaattcag agtcggatgc aatttttttc 7380
ttttcttttg ggcataagta gattgtcatt tgagtatttt tttccattaa tatttgtgta 7440
ttcttagatt gagactttga tatgtaaccc agatttctgt tgttaggaaa cacataccgt 7500
gtagcttatc agaagaaaaa gatctagcgt gcatctatat tctatcggac tatgatattt 7560
ggcacatgtg tgccatataa acaaaactgt cacacctcta tttccttcac tctaaataga 7620
tgtatacatt agtttgattc tttcttcttt cacgtgcatt tctccttcta attagatgca 7680
tacattagag gacaaaaaag acgatgtcat tctagatttt ctttattaaa atatttaaaa 7740
tgttttgtag ctcaaaccgt cggtccgatg taaaaaccgt ttttacatat acgattcgtc 7800
gcgatgagat ctttg 7815
<210> 161
<211> 7299
<212> DNA
<213> 人工序列
<220>
<223> 转化构建体
<400> 161
caatttcatc aagacatgcg tgattgggaa actcacgtgc agctgcaaaa taatttgatt 60
gagtatatgt gggtcatgtt ggcaaccaat aaatgtatct tcttttattc gtttgcaaaa 120
ctatgaaaca tttattttta tttgtatccg gtttgtaaaa ctatactatt ttattctgta 180
aactatgtta tctgacaata tgtttgaatg caaactaata taatattggg cggccaaccg 240
gccgcgttgg cacgttgggt gcgctgccga ctcaaatcta aaaaaagacg gacgccgggc 300
gggcggccga cccaaacgaa caaaaagcgg acgaaatcgc cgtccatttg ggtcgctcca 360
tcggagttgc tctgtctggc cgccaaatgg ggacgtgacg gacaccgagt gaactcgtgc 420
ggtgtgtccg cttcacatct aaatttaggt aaaaaaatga atccgtatgg acatgttttg 480
agaatgggtc gttacgttgc gacaatattt ttatccacaa cgacctaaac aaatgcgggc 540
agatgaaatg tatggctgcg ttggagatgc tgttaacgga gcaaagtcgt tggtagtcgg 600
accgagcaaa gtaattgctg aggcaggtag tggacgagac tgtagaaagg ttccacgctt 660
aatatcttga gcagtacttg tagcacatgt gtccctgcaa agctgtctcc gtgctgtata 720
ccatgtgaat tgcttttctc aaaagtagtg gagtacccta ccgtgtttca ttagtcttgc 780
cggcttcata gacaaaggat tcttttttcc cgtgccatcg ccatctgcaa gcccaaatca 840
acacacacca gagagagaga gagagagaga gagagagaga gagagagcaa caatggcgga 900
agttctggtg agcgcatcca caggggcaat gggttccctc ctgcggaagt tgggagctat 960
gttgacggat gaatacaagc tgctcaagaa cgtccgtggg gacatcaagt tcctcaagga 1020
cgagctggag gtcatgtgcg cgttcctcct caagatgtca gatgtggagg agcctgacga 1080
accaacaaag ctgcgtgtga cggcggtgcg ggagatgtcc tataagatag aggacaacat 1140
cgacaagttc atggtcctcg tggaacacga gtcctcctgc tccgaggcgg ctcatggcgt 1200
cgccaagctc atggacaagt gcaagaactt gctgccggac atcaagaccc gccgcaggat 1260
cgccaaggag gtcaaagaca tcaagaaaga aattaaggat gtcagcgaca gattttcaag 1320
gtaccagatc ttgttctctt tacagtacta ttagtaccta ccatcagatt tgtgaccgat 1380
ttgttgccac tcaaggtaca agattgacga gtcctcctcc tctatgccgg caaaaggcaa 1440
ggtcgaccct cgacttcgtg cagtctacaa agacgccgca gagctcgtcg gaattgatgg 1500
accaaaggat gaacttgtca agtggctgaa tgagaaagag ggccaatcac tgaaatctgt 1560
ctctattgtt ggatatggag ggttgggcaa gaccacgctc gccaaccaga tccgggtcaa 1620
ccttggagcg accttcgact gcggggcttt tgtctcgatt tcacgcaagc ctgacatgaa 1680
ggcgatatta agatctatat tatcgcaaat caccaagaaa gacgaggctt gctctagatt 1740
agatgatata caactcatca tcgacaagat tcgagaattc ctccaagaca caaggtatgt 1800
atgtgagatc tactacgaag taacattgct taattagctc atgcataatc cgttgcctag 1860
aattttgcta atgagattgc tttgtgtgat ttactttcgt cctcaacttg tatgactgcg 1920
cttaaacttt caagtcattg tttcttgcaa cttatgtcga tctgttcact aattaagata 1980
tagctaaaat gtgtttgcca atttctgtcg ccacacattt tctaatttga ttgccacagg 2040
tttgttaaac ttgcggctat gttctgttta ttaaataatt gtgacatggc gtcagtcaaa 2100
acaataaagt acatgcaatt ccctctcttt tgtacaagat aaatatccgc acgcactaca 2160
tgactgcaaa tatatctatc ttctaaggac ccaaagcact tgcatgtgca gaccattgtt 2220
accggatatg cagtacatta ggtttgtgat tagccacttt aagaacatag ggtttctaga 2280
ggctataaac ctacggttat cagtattata atcttgaatg gatatcaaac atccgttgaa 2340
gaatctatgc ccataacaca aaaagagaaa agggaagaat taatcatagt cagatcggac 2400
cagcctgact agcgtaacat aatgacaaca gtaggttgtt gcgtctttcc tggtttcgta 2460
gcaatttgca tgacgaattg aggcgcagag ccaccactca atatgtggtc ctaccaatca 2520
tgaggaaata acttgttacc aaaatgacaa aagaaaatag gtagtcccca tcattgtttc 2580
aatatctatc ctcttgcgaa catgctattg gcagtggcaa aacattgtac cttgccactg 2640
gcaacagctg cctttactcg gcctgagcat tcatattttt ttcctcttgg ttgttaaatc 2700
ttgctatttt tcgcttgcct tctatggcaa tctttttttt tcttatattg ccaccaaatt 2760
gtatctaaga gcaactccaa cacgccgacg caatttttgt ccatttagat cggccaagcg 2820
gacggggtgt ctggttttgc ctttgggtgg tgcgcctacg cattagtccg cgtgggcatt 2880
taaaaaactt aaaaagctta aaataattaa atataaataa aaatgcatat taaagcccgt 2940
caaagtccac ataattaaac ataatacatt aatatattaa taaaaaacag ccgccccagc 3000
ccgcagccct aggcgtcttc gtcggaggag tgggtgaggt cgaagaatgc cggcagctca 3060
gcccacggga aggcctgctg gtgggcagcc agcaccgcct gctccgctgt ctgcggcggc 3120
ggcggcccta cctggcaccg cgcacgcctc ctcctcgcgc tcctgccgct cctcctcctc 3180
atcgcacgcc caacgaagtt ttggcgctcc tccccctcgg gttcccgctg catgcggacc 3240
tggccgtcgt accgctcctc ctccagcgtg cggtccttcc acgccacgag gtaggccgcc 3300
tcctcctcca tcacgcacag ccaaatgggc ggcttgctca tccgcttgca gacgatgccc 3360
tgccattggt aaacgccggt gggcgcgggt ggtgctagcg ctggtgcagg cggtgacgat 3420
gtggctggtg gtcccggcag gtagacacta tagctggcgg ccgatagctg cagcgtgtca 3480
tcgaggccag accactagga gtcctcgcac taagactcct cggtgacgtg atgggccccc 3540
tcctccaatg ccttgaggaa cgccgcctcc tcctcaacct actatttgac caccggtggc 3600
ggcggtggag ggcaggcttc gagctggacg gcgtcgcgga ggcgcacatg gtgctcgatg 3660
gtgaaccaga cttctcagtt aggggagtct ggcctcgatg ggtggagtcg cgacgctgct 3720
ctggcgtcag gagagcacgc cgggcccgaa tctcgttggc gcgcgcacgc tcggactgtg 3780
gcaccgccgg cactggaatg tggtgggggt cgaggtgcca gccgtggggg aagttcacgt 3840
ccggccacgg cagtgagacg cggtactccc ggtggtacta cgcgtggtgc accgggatgt 3900
actggtggtc ccgtggcgct gccgccctcc catcccgcag cgccgacacc aatgcgaacc 3960
ccttcgctgc cggcctcacc ggcccttgca acgagccggc ctcgtggtcg tgcttgcctt 4020
gcccttacct ttgccaaaga aacccatgac ggctagggtt ttctgtcacc ggcgagggag 4080
cagatgtgga ctagagtggg aggagaagtg gatgaggccc gccccgccgc acccttgcaa 4140
ttaaaaaggg cgtgatccgg acgtttctag tcactgccat gcgggcccga ggtaggtggg 4200
cgccattgac tacgtctgtg gtggtagttg gctggcagac acgccagccc gaggcgcaca 4260
ccgagcggac gcgcaacacg tccgtctcgt gttcgtgtga acgcaaatca ggcacaaatt 4320
tgggctggaa atgcgtctac atggatgccg aacggacgtg atttgggttt gtgtccgcac 4380
gttgggccgg cgtttttgtg cctttcgacc ctaatggaca cacacggacc aaatccgttg 4440
gcgtgttaga gttgcttgta cctagtacta ctatgtgtgg agttgttatc tttgagtttc 4500
tcccccatct gtagctgtgt attattgtga gatgctctca ggatttcact tattttagat 4560
aatgacaact ttcttgatgc tagacaatta catcaaggtg tattatatct aggatgcaca 4620
cagatgtgag attgtttcag gattttaggt tacttggtag gtgttactat aaatatctaa 4680
tgagctctaa tgtgtatgtt catgaaactg tgttgcaggt actttatcat aattgatgat 4740
atatgggagt taggaacatg ggaaactttg aaatgtgcat ttgtcaagaa tacattgggc 4800
agcagaataa ttatcacaac acgtatagtt gatgttgcca aatcttgttc tccttctagt 4860
gaagatcttg tctatgagat gaaaccactc agtgaggctg actcgaagaa attgtttttc 4920
aagaggatat ttggctgtga agaaagctgt cctgatagct tgaaagaagc tgccaatgat 4980
attttgaaaa aatgccgtgg cctacctctg gccatcaatg ccatttctag cgtgttggtc 5040
acaacaaggg aaacaaaaga agagtgggat cgggtgcgac attcaatacg ctcttcaaag 5100
gtcaaaagtg atataataga gaccatgaat tacatattgt ctcttagtta ttttgatctt 5160
ccccaccacc taagaagctg cttattgtac ctggctctgt ttcctgaaga ccaattgatt 5220
ggaaggaagc ggttagtacg cagatggatt tctgaaggct ttatccatgg agaaagtgga 5280
caagatctca tggaattagg agaggaatat tttcaccagc ttgtgaacag aagtctaata 5340
cagcccggca acatagggta tgacggcaag gcaatgtatt gccgagtcca tgacaccatc 5400
cttgatttcc taattgacaa atcctctgaa gagaacatgt gtactgtcct aaagaaacaa 5460
tgcaagccta atggcatagt tcgtcggctc tctctaatgg ggaatgagga tgaagaaatc 5520
gtggagcaat tggatctgtc acacgctcgg tcaatcactg cttttgggga tatcaagcta 5580
ttgccttcgc ttgggaggtc aaaatgcttg cgcgtgctag acttgcaaga ctgcgatcag 5640
ttggaaaatc atcatatcaa ggatattgaa agactctatc agctaagata cttggatatc 5700
tcttctacag gaatcacgga gcttcccagg caaattgggg agttgctgta tctagagacg 5760
ctagttgcct atggattacg tgagcttcct gaaagcacaa gtcggctaca acgactggca 5820
cgtttgtttg tttactctgg ttgtaagctt ccaggtgggt tgggaaactt gataaacttg 5880
caagagcttg actgcgttga tgccttgcat ttgaagcatg tggaagagct cggcaaacta 5940
acaaatctga gaaagctgag tattaaattg gacactggtg ggattgaagg caacaaatta 6000
gaagagtcca aggaaaagct ggtgtcctct ctctgtaaac tggacgaatg cggcctcctt 6060
tccctgagta ttgattatta tctgagagaa aaggatggag aagagccgtt cctccctgct 6120
ttgggttgta tccaggaggt ttttgtatat ggacaagata tcagccggat tagcagatgg 6180
ctcgcgtcac ttcccaacct acacgggttg cttttggaca atccgaagat agagcagcag 6240
gatattgaga tgattggatt gatacccaat ctgatcgatt tgacactgta tttacgcata 6300
acagacgacg ctgggcggct catcatcaag cgtgaaggat ttcaacagtt gcagaggttt 6360
gagctttccc gtattcgtat gggggattta atgtttgaac cgggggctat gccgaggctc 6420
aaggagctta tactctacca cttcatcgaa aagcccaaat ctggtgcggt cgatttcgac 6480
tttggcatcc aacacctctc cagccttgct cgcctcactg tcggcttatt atgcgttggc 6540
tcgacggcgg cggaagtaga ggctgcggag gatgctttca agagcatggc cgaggcgaac 6600
cccaaccgtc caatactgga gatgacaaga ctttatccgc agcatatgtt acaagacgag 6660
caaataggta tgacaggctc aggtactatt aactagtttg tacttttgtc ttggtgagca 6720
gtagatcaaa agatgtgcat atgcttcaat cttttttccg gtaatctatt tcagctacaa 6780
cgccggccgt caagtcctag atgcctaaac cgagatgcgc ctttggttat ggagcccaag 6840
tcacgtactc tgattggatg ctgcagcaaa tgaagctaca catttctatt cgggctacct 6900
cttttttttt gaaactctat tcgggctacc tctttgtctc aaagctggtg tactcttgtg 6960
atttgtgttg cgcaactgat acttctgtgt atgtctgcaa gtatcacgaa ttagtgggaa 7020
tgactaggaa taagtaagac aagtatcacg aattcagagt cacatgcaat ttttttcttt 7080
tcttttgggc ataagtagat tgccatttga gtattttttc cattaatatt tgcgtattct 7140
tagattggga cttagatatg taacccagat ttctgttgtt aggaaacaca taccgtgtag 7200
cttatcaaaa gaaaaagatc tttagagaaa ataacatcat tagattgttc aacttggatg 7260
aaaaatgtga aaatggccaa accataatta aataacatc 7299
<210> 162
<211> 6481
<212> DNA
<213> 人工序列
<220>
<223> 转化构建体
<400> 162
agtactgtaa taattgattc cgtcgcgcct ctgcgtccac cgcccaacag cccaaatcgc 60
cgccgcctcg ctcttcccta ctccggccac cgccgccgcc gacggccacg ctccgctgtt 120
tccccccacc ccacccctcc agcaccatgc ctctgttcct cgttgccaca tcggtaacct 180
gccccacagg cggccccacc gtgctgctgt ggggtggccg tcgatgtcgc cggcagggtc 240
ctcccgcgtg cttctccaag cgacctctgc cagctcctcc cttgtctcgc gtcgcctgcc 300
atgcgtggct cggacccaaa atcttttcgc ccgttcgcgg agccgggtag ggaggggggc 360
ggagatgctc tgaacttgaa gccgagatat ttggagtgca gcagatggac tcatttttct 420
aagagcgcac atgctatata tcagcggtaa atttcctcat acattgttgc taacggagca 480
agatttgtag ccaaacgaag atttgttttc ctggtaacga aggattctgt attgtttcac 540
ctacccataa caaaaagata acgcatttct gtatggcagg gctgcgtggt ggtcgttgaa 600
gtcgccggcc ccgctgtccc atttttttcc acacgccatc gtccacttgc tctgaatctg 660
cctaactcca gaccatttct tccacacgtt gctcttatcc ttttgcgcgt gcttccgcgc 720
ggtctcatcc tgttgacctg ctaaatctgc acaactacat gccatttctc ttctgcatag 780
atctcacgct ctcatccagg tttcctggat ctgcacagct gcaggccatt tctcttctgc 840
atagcgcttg tagtgtttcc ctgtgtttgc agtgtgagag ctaaaatggc ggagtttgta 900
gtcaggccac tggtatccac gttgatgaac acggcatcca gctacctgct ggaccagtac 960
aaggtgatgg atggcatgaa ggagcagcgc gagaccctca agcgccagct gccagccatc 1020
ttggacatca tccaagatgc ggagaagaaa ggagcttccc tacctggagt gcgcgcttgg 1080
ctcgaagcac tcaagaaggt ggcctatgag gcgaacgatg tctttgatga gttcaagtac 1140
gaggccctcc ggcgtgacgc caagaagaag ggacactaca agaagcttgg ctttgacatc 1200
gtaagcctct tccctgctca caaccccatt gtatttcgtt acaggatggg caagaagttg 1260
tgcaggattg tgcgcaaaat tgagggcctt gtcagagaga tgaatgactt cggattcaac 1320
caaactcagc aagcaccacc atccaagcag tggcgcaaca cagattccat aataattgac 1380
tctgagaagg atattgtcag caggtccaga aacgaagaga agaagaagat cgttgatata 1440
ttgattgatc aagctggcga tagggacctc atcgtccttc ccattgttgg aatgggcggg 1500
ctgggcaaga ccacctttgc tcagcttgtc tacaatgacc ctataatcaa ggagcatttc 1560
aagctccaga ggtggtgttg tgtgtcagat gattttgatg ttgtgaagat tgcaaacaac 1620
atctgtgaga ccaatgagat ccatcgtgaa aaggcattgc agaaccttca gaaagaagta 1680
agtgggaaga gatacctcat tgtgttggat gatgtatgga atgaagatgc tgataagtgg 1740
gaaaaactga aaacctgcct gaagcacggt gggaagggga gtgcaatatt gacgaccact 1800
cgcaatgtgc aagtagcacg aattatgaag atgtgtattg ctgatagcca taatctcaga 1860
aatctagata aagtatttct gaaggaaata tttgagaata gagcattctg tttgcaaaag 1920
ccaaaggctg ctgagctaag cgatgtggtt gataagatta tggacagatg tggaggctct 1980
cctttagctg ccaaagcctt tgggtctatg ttgagtaaca agactagcat gaaagaatgg 2040
acagacatac tagccagaag caacacttgc aatgagggca caaaaacttt tcttgtactc 2100
aagctcagct atgatgactt gccatcacac ttgaaacaat gctttgcttt ttgtgctgta 2160
tttcccaaag attatgagat tggtgttgaa acattaattc agctatggat ggcacatgat 2220
ttcataccac tgaaagaagg tgataatctt gagaaggtag gtagagaaat ttttgatgag 2280
ttaacttgga ggtcattctt tcaagatgtc aagcgaatcc ctcgaagaga gtggtggggg 2340
gagctccgtc ctagaacaat atgcaagata catgatctta tgcatgacat tgccttgtct 2400
gttatgggaa aagattgtct tactatagtt gataggccta atgaaaagga gttactgtcc 2460
actggtccta cacgttactt gttctcatca tatgagtata tcggaactct gttggatgat 2520
tatctgaaga aacactctcc cgctctccag acactgctgt atccgtatcc ctacaccagt 2580
gactcagcac cacacttgtc gaagtgcaat tatctgcgag cactgcaact cttcagtttg 2640
agaaaacttc cactctggcc aaggcactta cagcacctga ggtatcttga tctctcaaat 2700
aacatgttga tcgaagagct tcccaaggaa ataagcatac tgtataatct acagactctt 2760
aacctttgta actgcagaag gcttgatcaa cttccagagg atatgaagta tatggaaaat 2820
ctccgtcacc tctatacaaa tggatgctca tcattgaagt gcatgcctcc aggcctcggg 2880
caactcactt ctctacagac tctaacatat tttgtggtga gctctagtcc tggttgcagt 2940
actattagag aactacagga cttgaatctt ggtggtgagt tagagctatc tcgtcttcag 3000
tttgcaactg aagtagatgc taaagcatgc agccttggaa ataaagagaa acttacacat 3060
ttatctctta aatggggtga tgacagcagt gatgaattgg gccaccacag gaatgtgctc 3120
gatgctctta aacctcatgc tgtcctggag tttctaagaa tacgctccta tagaggtact 3180
ggcttccccg catgggtggt aagtattaat tttctgcagc atttgaccga gctccagcta 3240
gatggttgta caatgtgtga ggaatttccc caattcggtc aatttaagtc ccttgaagtt 3300
cttgttttga aaaggttgaa caaacttcaa agcctatgca atcacagttc atctgcaata 3360
tttccagcat taaaagttct cagattaaaa aaattggaga tttttgagag atgggtggca 3420
acagaaggag aagagttagc atttcctcaa cttgagaatg ttaaaattaa ggactgccca 3480
aagctagcaa ttctgcctga agcaccaaaa ctcaagttta tagctctaaa agaagaaaag 3540
gcacagctat ccttatcaat atttaagtcc aggtatatgg cttgcttgtc tggggttggc 3600
ctgtccgtcc gtgatacaga agcagcacca agaacagagc ttgatcagga ttgtgaagta 3660
tctctttcga acctgctgtt ggatggttgc aactttttgt tctgctcaac tccattgcag 3720
ccaactgttg gggtctggaa atggtttggt cagcttgtgc atttggaaat aaaatcttgt 3780
gatatgctca tctactggcc tgaagaagag ttccgttgct tggtatcatt gaatagttta 3840
tccattaatt cctgcagcaa gctagtagga cacacccagg ggaaaggatg ccgtacccgt 3900
actcaagtaa gggatcaact cctgccaaat cttaaaaatt taagaataca tcattgtgga 3960
agtttgacag agctttttgt tctgccgcca tctctcacaa gtattgatat gctggattgc 4020
aatagtattg agtccatact ggggcaggat gatacagagt tggagagtat actacacttt 4080
gatacagcat catcatcgga acacttcaat gacctcacgt ccacatgttt gctagagcag 4140
tcactttcac ccagaattaa tcctttgcca tgtcttgatt atttgcgtat agttagctgt 4200
aagaagcttc gtttcgtgcc agtgcagtta gatgcactcc aatttttgtg tatttctgat 4260
tgcaatgggc tggagtcgct ggattgtttg ggagacctac cgtcactgga atatctatac 4320
cttatgggct gcaaacatct ggcatctgta ccaggaagtc ttgggagtta ctcggccctt 4380
cagaagctcc gcattgaata ctgcccagct ttaaatatga agccacttca tggacatctc 4440
caacagcggc ttgatagcct tgaattgaag gatctatcta aggctggttc aagccatcct 4500
aatgaaggta cctttcaatt tcatttaccc cattttgtat ctctttttcc atctgtatag 4560
tttacagatg tacatattgc agcctgtagt ttcaagttta tccaactcct tgtattttca 4620
catttggtat gtagtgaccg cgtacacagt ttctctgata tggctattcc aatatcattt 4680
atttgtatac cgtaagtttg acttggattg acgtcgtcat gtgtggccga tctgtatttt 4740
ctatcatttt ggtaaaagtt aatacaccat ggaagtatgc tctcttgtgt agtgaccgta 4800
cacagatcat tcttttatgt ctatcccact atcatttatt tgtaaagttt gacttgctat 4860
taatgttcac agagatattc tgacattgtt ttccatgtac agggcctaaa ctatgggaac 4920
caaaatcctg gaaatatatg attccttcac tgcgcaagcg tgagtctgaa tagagataca 4980
actaccatgt atgtttctgg tttttttttc ttgcaacttt gattctaaaa gtttgtaggc 5040
taaaagatcg gttttggtat gttcaccatt cttgacctga atgtgtttaa tttccttgca 5100
gggtcgctcg tgtgcgcata caagtgatga acacaattgg taactgccct cttcaaggga 5160
gtcaagttct tttatttgcc tgctccgtga gctctgatga aaataaggcg ctcgaaggcc 5220
tgaagcatgg gggaaaatga acttcaactt gcagaactgg agagagaagg tcgtgctgaa 5280
tcgtgtttac tttgtgcaga acaaacaagc atgtccttgg gcaaatcgtg cggactataa 5340
gtgcacttgc agtcgtgtgc tggcgatcga gccttttgtt ttccatgtgt acttacagtt 5400
caccgtagta atacggtgtg ctatttcaca tcttttacat aatctttaga gcagagcacg 5460
atcctggtta attcagacag ctgattagca gacatctggt tagttcagag ttagagattg 5520
tgtgcgtgtg tttatggctg cttcagagag ctgattgtgt gcgtgttttt gtgcgtgcac 5580
gacgtgctag tatctgcttg ccaattcgcc atgtacacaa aagtctgcaa ccaaacttgg 5640
aagatctcaa attaccagtg cctttcagga tgaacataca gtacatgatt tcagaagagt 5700
agagtgcagg tgccagcttc caccattttg ttctgccgca aaccagagct gatcgcagca 5760
aagaaattat atagacagcc atggtagtat agtaagctca ctctagttct tcagttccag 5820
caggcacaca tgtcctctca acagaacaga acagaaggga acagccccac accgcaggct 5880
gttgtcaccg actcaccgtg cacaaacctg cttccgtcca ttttacgacg gtccgtcgct 5940
tgccatctct agcttccctt ttttttgata gaaagaccgt aatggaaccc cctacagtat 6000
aattggtgca acaaatccaa attgcaagta ccataccttg aatctgggtg ggtgggaagg 6060
catcaacccc tccccatcac taggctatgc cttagtctgc catctctagc ttccctaccg 6120
accagggaat cacggctcgt gctacccctt ttagcacccc gacgaggagt agcttctgca 6180
cggattatag tggggaagaa aatgtatgac taggatacag gaattccaga gaagccaaat 6240
tcaggaaaca accaatccct ttattttaca aagccgccca taaacctttt tttaataaaa 6300
catggtagta cggggatttt attttaataa acaaccaatc cccttctttt acctggtatt 6360
atgattttat ccattcccca ataaccaaca taatttcttc taccaaaaaa acaacccttg 6420
gtgatgaatt tattcagcat aatgagaaga acatgtataa ctgcaatgca gcaggaattt 6480
c 6481
<210> 163
<211> 12150
<212> DNA
<213> 人工序列
<220>
<223> 载体
<400> 163
gtcgacgatc gtcaacgttc acttctaaag aaatagcgcc actcagcttc ctcagcggct 60
ttatccagcg atttcctatt atgtcggcat agttctcaag atcgacagcc tgtcacggtt 120
aagcgagaaa tgaataagaa ggctgataat tcggatctct gcgagggaga tgatatttga 180
tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc 240
cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca 300
aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg 360
tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca 420
ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt 480
ttttaatgta ctgaattcca atccccaaaa atctgagctt aacagcacag ttgctcctct 540
cagagcagaa tcgggtattc aacaccctca tatcaactac tacgttgtgt ataacggtcc 600
acatgccggt atatacgatg actggggttg tacaaaggcg gcaacaaacg gcgttcccgg 660
agttgcacac aagaaatttg ccactattac agaggcaaga gcagcagctg acgcgtacac 720
aacaagtcag caaacagaca ggttgaactt catccccaaa ggagaagctc aactcaagcc 780
caagagcttt gctaaggccc taacaagccc accaaagcaa aaagcccact ggctcacgct 840
aggaaccaaa aggcccagca gtgatccagc cccaaaagag atctcctttg ccccggagat 900
tacaatggac gatttcctct atctttacga tctaggaagg aagttcgaag gtgaaggtga 960
cgacactatg ttcaccactg ataatgagaa ggttagcctc ttcaatttca gaaagaatgc 1020
tgacccacag atggttagag aggcctacgc agcaggtctc atcaagacga tctacccgag 1080
taacaatctc caggagatca aataccttcc caagaaggtt aaagatgcag tcaaaagatt 1140
caggactaat tgcatcaaga acacagagaa agacatattt ctcaagatca gaagtactat 1200
tccagtatgg acgattcaag gcttgcttca taaaccaagg caagtaatag agattggagt 1260
ctctaaaaag gtagttccta ctgaatctaa ggccatgcat ggagtctaag attcaaatcg 1320
aggatctaac agaactcgcc gtgaagactg gcgaacagtt catacagagt cttttacgac 1380
tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca cgacactctg gtctactcca 1440
aaaatgtcaa agatacagtc tcagaagacc aaagggctat tgagactttt caacaaagga 1500
taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc atcgaaagga 1560
cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggctatca 1620
ttcaagatct ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg 1680
tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gacatctcca 1740
ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc tctatataag 1800
gaagttcatt tcatttggag aggacacgct cgagtataag agctctattt ttacaacaat 1860
taccaacaac aacaaacaac aaacaacatt acaattacta tttacaatta ccatgagccc 1920
agaacgacgc ccggccgaca tccgccgtgc caccgaggcg gacatgccgg cggtctgcac 1980
catcgtcaac cactacatcg agacaagcac ggtcaacttc cgtaccgagc cgcaggaacc 2040
gcaggagtgg acggacgacc tcgtccgtct gcgggagcgc tatccctggc tcgtcgccga 2100
ggtggacggc gaggtcgccg gcatcgccta cgcgggcccc tggaaggcac gcaacgccta 2160
cgactggacg gccgagtcaa ccgtgtacgt ctccccccgc caccagcgga cgggactggg 2220
ctccacgctc tacacccacc tgctgaagtc cctggaggca cagggcttca agagcgtggt 2280
cgctgtcatc gggctgccca acgacccgag cgtgcgcatg cacgaggcgc tcggatatgc 2340
cccccgcggc atgctgcggg cggccggctt caagcacggg aactggcatg acgtgggttt 2400
ctggcagctg gacttcagcc tgccggtacc gccccgtccg gtcctgcccg tcaccgagat 2460
ttgatgaccc gggggatcct agagtcctgc tttaatgaga tatgcgagac gcctatgatc 2520
gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct 2580
cagatcctta ccgccggttt cggttcattc taatgaatat atcacccgtt actatcgtat 2640
ttttatgaat aatattctcc gttcaattta ctgattgtac cctactactt atatgtacaa 2700
tattaaaatg aaaacaatat attgtgctga ataggtttat agcgacatct atgatagagc 2760
gccacaataa caaacaattg cgttttatta ttacaaatcc aattttaaaa aaagcggcag 2820
aaccggtcaa acctaaaaga ctgattacat aaatcttatt caaatttcaa aagtgcccca 2880
ggggctagta tctacgacac accgagcggc gaactaataa cgctcactga agggaactcc 2940
ggttccccgc cggcgcgcat gggtgagatt ccttgaagtt gagtattggc cgtccgctct 3000
accgaaagtt acgggcacca ttcaacccgg tccagcacgg cggccgggta accgacttgc 3060
tgccccgaga attatgcagc atttttttgg tgtatgtggg ccccaaatga agtgcaggtc 3120
aaaccttgac agtgacgaca aatcgttggg cgggtccagg gcgaattttg cgacaacatg 3180
tcgaggctca gcaggacctg caggcatgca agctctagag cggccgccac cgcggctgag 3240
tggctccttc aagcttgcgg atccgggcgg aaataggtaa agaagttgcg gataaggtaa 3300
ttgccattgc agattatttg gattgagagt gaatatgaga ctctaattgg ataccgaggg 3360
gaatttatgg aacgtcagtg gagcattttt gacaagaaat atttgctagc tgatagtgac 3420
cttaggcgac ttttgaacgc gcaataatgg tttctgacgt atgtgcttag ctcattaaac 3480
tccagaaacc cgcggctgag tggctccttc aacgttgcgg ttctgtcagt tccaaacgta 3540
aaacggcttg tcccgcgtca tcggcggggg tcataacgtg actcccttaa ttctccgctc 3600
atgatcagat tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac aggatatatt 3660
ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt 3720
gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg 3780
agtgcttggc attccgtgcg ataatgactt ctgttcaacc acccaaacgt cggaaagcct 3840
gacgacggag cagcattcca aaaagatccc ttggctcgtc tgggtcggct agaaggtcga 3900
gtgggctgct gtggcttgat ccctcaacgc ggtcgcggac gtagcgcagc gccgaaaaat 3960
cctcgatcgc aaatccgacg ctgtcgaaaa gcgtgatctg cttgtcgctc tttcggccga 4020
cgtcctggcc agtcatcacg cgccaaagtt ccgtcacagg atgatctggc gcgagttgct 4080
ggatctcgcc ttcaatccgg gtctgtggcg ggaactccac gaaaatatcc gaacgcagca 4140
agatcgtcga cccctttccg acgctcaccg ggctggttgc cctcgccgct gggctggcgg 4200
ccgtctatgg ccctgcaaac gcgccagaaa cgccgtcgaa gccgtgtgcg agacaccgcg 4260
gccggccgcc ggcgttgtgg atacctcgcg gaaaacttgg ccctcactga cagatgaggg 4320
gcggacgttg acacttgagg ggccgactca cccggcgcgg cgttgacaga tgaggggcag 4380
gctcgatttc ggccggcgac gtggagctgg ccagcctcgc aaatcggcga aaacgcctga 4440
ttttacgcga gtttcccaca gatgatgtgg acaagcctgg ggataagtgc cctgcggtat 4500
tgacacttga ggggcgcgac tactgacaga tgaggggcgc gatccttgac acttgagggg 4560
cagagtgctg acagatgagg ggcgcaccta ttgacatttg aggggctgtc cacaggcaga 4620
aaatccagca tttgcaaggg tttccgcccg tttttcggcc accgctaacc tgtcttttaa 4680
cctgctttta aaccaatatt tataaacctt gtttttaacc agggctgcgc cctggcgcgt 4740
gaccgcgcac gccgaagggg ggtgcccccc cttctcgaac cctcccggcc cgctaacgcg 4800
ggcctcccat ccccccaggg gctgcgcccc tcggccgcga acggcctcac cccaaaaatg 4860
gcagccaagc tcctaacatt ttattagaga gcaggctagt tgcttagata catgatcttc 4920
aggccgttat ctgtcagggc aagcgaaaat tggccattta tgacgaccaa tgccccgcag 4980
aagctcccat ctttgccgcc atagacgccg cgcccccctt ttggggtgta gaacatcctt 5040
ttgccagatg tggaaaagaa gttcgttgtc ccattgttgg caatgacgta gtagccggcg 5100
aaagtgcgag acccatttgc gctatatata agcctacgat ttccgttgcg actattgtcg 5160
taattggatg aactattatc gtagttgctc tcagagttgt cgtaatttga tggactattg 5220
tcgtaattgc ttatggagtt gtcgtagttg cttggagaaa tgtcgtagtt ggatggggag 5280
tagtcatagg gaagacgagc ttcatccact aaaacaattg gcaggtcagc aagtgcctgc 5340
cccgatgcca tcgcaagtac gaggcttaga accaccttca acagatcgcg catagtcttc 5400
cccagctctc taacgcttga gttaagccgc gccgcgaagc ggcgtcggct tgaacgaatt 5460
gttagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg aacaaattct 5520
tccaactgat ctgcgcgcga ggccaagcga tcttcttgtc caagataagc ctgcctagct 5580
tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca 5640
tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact 5700
acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt 5760
agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct 5820
accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc 5880
gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt 5940
tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct 6000
acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc 6060
aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca 6120
ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc 6180
ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg 6240
atcaccgctt ccctcatgat gtttaactcc tgaattaagc cgcgccgcga agcggtgtcg 6300
gcttgaatga attgttaggc gtcatcctgt gctcccgaga accagtacca gtacatcgct 6360
gtttcgttcg agacttgagg tctagtttta tacgtgaaca ggtcaatgcc gccgagagta 6420
aagccacatt ttgcgtacaa attgcaggca ggtacattgt tcgtttgtgt ctctaatcgt 6480
atgccaagga gctgtctgct tagtgcccac tttttcgcaa attcgatgag actgtgcgcg 6540
actcctttgc ctcggtgcgt gtgcgacaca acaatgtgtt cgatagaggc tagatcgttc 6600
catgttgagt tgagttcaat cttcccgaca agctcttggt cgatgaatgc gccatagcaa 6660
gcagagtctt catcagagtc atcatccgag atgtaatcct tccggtaggg gctcacactt 6720
ctggtagata gttcaaagcc ttggtcggat aggtgcacat cgaacacttc acgaacaatg 6780
aaatggttct cagcatccaa tgtttccgcc acctgctcag ggatcaccga aatcttcata 6840
tgacgcctaa cgcctggcac agcggatcgc aaacctggcg cggcttttgg cacaaaaggc 6900
gtgacaggtt tgcgaatccg ttgctgccac ttgtttaata gactggatgg aggcggataa 6960
agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 7020
tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 7080
ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 7140
acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 7200
ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 7260
gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 7320
gtcagacccc gtagaaaaga tcaaaggatc ttcttgatat cctttttttc tgcgcgtaat 7380
ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 7440
gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 7500
ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 7560
cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 7620
cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 7680
ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 7740
tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 7800
cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 7860
ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 7920
aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 7980
ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 8040
tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga 8100
gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg 8160
cggtatttca caccgcatat gaagatcggc ggcaatagct tcttagcgcc atcccggctg 8220
agaaagccca gtaaggaaac aactgtaggt tcgagtcgcg agatcccccg gaaccaaagg 8280
aagtaggtta aacccgctcc gatcaggccg agccacgcca ggccgagaac attggttcct 8340
gtaggcatcg ggattggcgg atcaaacact aaagctactg gaacgagcag aagtcctccg 8400
gccgccagtt gccaggcggt aaaggtgagc agaggcacgg gaggttgcca cttgcgggtc 8460
agcacggttc cgaacgccat ggaaaccgcc cccgccaggc ccgctgcgac gccgacagga 8520
tctagcgctg cgtttggtgt caacaccaac agcgccacgc ccgcagttcc gcaaatagcc 8580
cccaggaccg ccatcaatcg tatcgggcta cctagcagag cggcagagat gaacacgacc 8640
atcagcggct gcacagcgcc taccgtcgcc gcgaccccgc ccggcaggcg gtagaccgaa 8700
ataaacaaca agctccagaa tagcgaaata ttaagtgcgc cgaggatgaa gatgcgcatc 8760
caccagattc ccgttggaat ctgtcggacg atcatcacga gcaataaacc cgccggcaac 8820
gcccgcagca gcataccggc gacccctcgg cctcgctgtt cgggctccac gaaaacgccg 8880
gacagatgcg ccttgtgagc gtccttgggg ccgtcctcct gtttgaagac cgacagccca 8940
atgatctcgc cgtcgatgta ggcgccgaat gccacggcat ctcgcaaccg ttcagcgaac 9000
gcctccatgg gctttttctc ctcgtgctcg taaacggacc cgaacatctc tggagctttc 9060
ttcagggccg acaatcggat ctcgcggaaa tcctgcacgt cggccgctcc aagccgtcga 9120
atctgagcct taatcacaat tgtcaatttt aatcctctgt ttatcggcag ttcgtagagc 9180
gcgccgtgcg tcccgagcga tactgagcga agcaagtgcg tcgagcagtg cccgcttgtt 9240
cctgaaatgc cagtaaagcg ctggctgctg aacccccagc cggaactgac cccacaaggc 9300
cctagcgttt gcaatgcacc aggtcatcat tgacccaggc gtgttccacc aggccgctgc 9360
ctcgcaactc ttcgcaggct tcgccgacct gctcgcgcca cttcttcacg cgggtggaat 9420
ccgatccgca catgaggcgg aaggtttcca gcttgagcgg gtacggctcc cggtgcgagc 9480
tgaaatagtc gaacatccgt cgggccgtcg gcgacagctt gcggtacttc tcccatatga 9540
atttcgtgta gtggtcgcca gcaaacagca cgacgatttc ctcgtcgatc aggacctggc 9600
aacgggacgt tttcttgcca cggtccagga cgcggaagcg gtgcagcagc gacaccgatt 9660
ccaggtgccc aacgcggtcg gacgtgaagc ccatcgccgt cgcctgtagg cgcgacaggc 9720
attcctcggc cttcgtgtaa taccggccat tgatcgacca gcccaggtcc tggcaaagct 9780
cgtagaacgt gaaggtgatc ggctcgccga taggggtgcg cttcgcgtac tccaacacct 9840
gctgccacac cagttcgtca tcgtcggccc gcagctcgac gccggtgtag gtgatcttca 9900
cgtccttgtt gacgtggaaa atgaccttgt tttgcagcgc ctcgcgcggg attttcttgt 9960
tgcgcgtggt gaacagggca gagcgggccg tgtcgtttgg catcgctcgc atcgtgtccg 10020
gccacggcgc aatatcgaac aaggaaagct gcatttcctt gatctgctgc ttcgtgtgtt 10080
tcagcaacgc ggcctgcttg gcctcgctga cctgttttgc caggtcctcg ccggcggttt 10140
ttcgcttctt ggtcgtcata gttcctcgcg tgtcgatggt catcgacttc gccaaacctg 10200
ccgcctcctg ttcgagacga cgcgaacgct ccacggcggc cgatggcgcg ggcagggcag 10260
ggggagccag ttgcacgctg tcgcgctcga tcttggccgt agcttgctgg accatcgagc 10320
cgacggactg gaaggtttcg cggggcgcac gcatgacggt gcggcttgcg atggtttcgg 10380
catcctcggc ggaaaacccc gcgtcgatca gttcttgcct gtatgccttc cggtcaaacg 10440
tccgattcat tcaccctcct tgcgggattg ccccgactca cgccggggca atgtgccctt 10500
attcctgatt tgacccgcct ggtgccttgg tgtccagata atccacctta tcggcaatga 10560
agtcggtccc gtagaccgtc tggccgtcct tctcgtactt ggtattccga atcttgccct 10620
gcacgaatac cagctccgcg aagtcgctct tcttgatgga gcgcatgggg acgtgcttgg 10680
caatcacgcg caccccccgg ccgttttagc ggctaaaaaa gtcatggctc tgccctcggg 10740
cggaccacgc ccatcatgac cttgccaagc tcgtcctgct tctcttcgat cttcgccagc 10800
agggcgagga tcgtggcatc accgaaccgc gccgtgcgcg ggtcgtcggt gagccagagt 10860
ttcagcaggc cgcccaggcg gcccaggtcg ccattgatgc gggccagctc gcggacgtgc 10920
tcatagtcca cgacgcccgt gattttgtag ccctggccga cggccagcag gtaggccgac 10980
aggctcatgc cggccgccgc cgccttttcc tcaatcgctc ttcgttcgtc tggaaggcag 11040
tacaccttga taggtgggct gcccttcctg gttgggtaat gactccaact tattgatagt 11100
gttttatgtt cagataatgc ccgatgactt tgtcatgcag ctccaccgat tttgagaacg 11160
acagcgactt ccgtcccagc cgtgccaggt gctgcctcag attcaggtta tgccgctcaa 11220
ttcgctgcgt atatcgcttg ctgattacgt gcagctttcc cttcaggcgg gattcataca 11280
gcggccagcc atccgtcatc catatcacca cgtcaaaggg tgacagcagg ctcataagac 11340
gccccagcgt cgccatagtg cgttcaccga atacgtgcgc aacaaccgtc ttccggagac 11400
tgtcatacgc gtaaaacagc cagcgctggc gcgatttagc cccgacatag ccccactgtt 11460
cgtccatttc cgcgcagacg atgacgtcac tgcccggctg tatgcgcgag gttaccgact 11520
gcggcctgag ttttttaagt gacgtaaaat cgtgttgagg ccaacgccca taatgcgggc 11580
tgttgcccgg catccaacgc cattcatggc catatcaatg attttctggt gcgtaccggg 11640
ttgagaagcg gtgtaagtga actgcagttg ccatgtttta cggcagtgag agcagagata 11700
gcgctgatgt ccggcggtgc ttttgccgtt acgcaccacc ccgtcagtag ctgaacagga 11760
gggacagctg atagaaacag aagccactgg agcacctcaa aaacaccatc atacactaaa 11820
tcagtaagtt ggcagcatca cccctggttg gcttggtttc atcagccatc cgcttgccct 11880
catctgttac gccggcggta gccggccagc ctcgcagagc aggattcccg ttgagcaccg 11940
ccaggtgcga ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac 12000
ctatcctgcc cggctgacgc cgttggatac accaaggaaa gtctacacga accctttggc 12060
aaaatcctgt atatcgtgcg aaaaaggatg gatataccga aaaaatcgct ataatgaccc 12120
cgaagcaggg ttatgcagcg gaaaagatcc 12150

Claims (53)

1.包含核苷酸序列的核酸分子，其中所述核苷酸序列选自：
(a)SEQ ID NO:1、4、7、10、19、22、25、34或49中列出的核苷酸序列；
(b)编码SEQ ID NO:2、5、8、11、20、23、26、35或50中列出的氨基酸序列的核苷酸序列，并且任选地，其中所述核苷酸序列不是天然形成的；
(c)SEQ ID NO:3、6、9、12、21、24、27、36或51中列出的核苷酸序列；
(d)与(a)或(c)中列出的核苷酸序列的至少一种具有至少85％序列一致性的核苷酸序列，其中所述核酸分子能够赋予包含所述核酸分子的小麦植物以抵抗秆锈病的抗性，并且任选地，其中所述核苷酸序列不是天然形成的；以及
(e)包含编码氨基酸序列的核苷酸序列的核酸分子，其中所述氨基酸序列与(c)中列出的至少一种氨基酸序列具有至少85％序列一致性，其中所述核酸分子能够赋予包含所述核酸分子的小麦植物以抵抗秆锈病的抗性，并且任选地，其中所述核苷酸序列不是天然形成的。
2.权利要求1的核酸分子，其中所述核酸分子为分离的核酸分子。
3.权利要求1或2的核酸分子，其中(d)或(e)的所述核酸分子编码包含卷曲螺旋结构域、核苷酸结合结构域和富含亮氨酸重复结构域的蛋白质。
4.权利要求3的核酸分子，其中所述卷曲螺旋结构域、所述核苷酸结合结构域和所述富含亮氨酸重复结构域的至少一种包含与SEQ ID NO:2或50中对应的结构域具有至少95％、96％、97％、98％、99％或100％的氨基酸序列。
5.权利要求3或4的核酸分子，其中所述富含亮氨酸重复结构域包含选自以下的氨基酸：在对应于SEQ ID NO:2的氨基酸607的氨基酸位置处的丝氨酸(S)、在对应于SEQ ID NO:2的氨基酸650的氨基酸位置处的酪氨酸(Y)、在对应于SEQ ID NO:2的氨基酸651的氨基酸位置处的丝氨酸(S)，以及在对应于SEQ ID NO:2的氨基酸657的氨基酸位置处的甘氨酸(G)。
6.表达盒，其包含权利要求1-5中任意一项的核酸分子以及可操作地连接的异源启动子。
7.载体，其包含权利要求1-5中任意一项的核酸分子或者权利要求6的表达盒。
8.权利要求7的载体，其进一步包含其他的小麦秆锈病抗性基因。
9.权利要求8的载体，其中所述其他的小麦秆锈病抗性基因选自Sr26、Sr32、Sr33、Sr39、Sr40、Sr47和Sr50。
10.使用权利要求1-5中任意一项的核酸分子、权利要求6的表达盒或权利要求7-9中任意一项的载体转化的宿主细胞。
11.权利要求10的宿主细胞，其中所述宿主细胞为植物细胞、细菌、真菌细胞或动物细胞。
12.权利要求10或11的宿主细胞，其中所述宿主细胞不是野生一粒小麦或栽培一粒小麦。
13.使用权利要求1-5中任意一项的核酸分子、权利要求6的表达盒或权利要求7-9中任意一项的载体转化的植物。
14.权利要求13的植物，其中所述植物为小麦植物。
15.权利要求14的植物，其中所述小麦植物不是野生一粒小麦或栽培一粒小麦。
16.包含在其基因组中稳定地引入多核苷酸构建体的转基因植物或种子，其中所述多核苷酸构建体包含选自以下的核苷酸序列：
(a)SEQ ID NO:1、4、7、10、19、22、25、34或49中列出的核苷酸序列；
(b)编码SEQ ID NO:2、5、8、11、20、23、26、35或50中列出的氨基酸序列的核苷酸序列，并且任选地，其中所述核苷酸序列不是天然形成的；
(c)SEQ ID NO:3、6、9、12、21、24、27、36或51中列出的核苷酸序列；
(d)与(a)或(c)中列出的核苷酸序列的至少一种具有至少85％序列一致性的核苷酸序列，其中所述核酸分子能够赋予包含所述核酸分子的小麦植物以抵抗秆锈病的抗性，并且任选地，其中所述核苷酸序列不是天然形成的；以及
(e)包含编码氨基酸序列的核苷酸序列的核酸分子，其中所述氨基酸序列与(c)中列出的至少一种氨基酸序列具有至少85％序列一致性，其中所述核酸分子能够赋予包含所述核酸分子的小麦植物以抵抗秆锈病的抗性，并且任选地，其中所述核苷酸序列不是天然形成的。
17.权利要求16的转基因植物或种子，其中(d)或(e)的所述核酸分子编码包含卷曲螺旋结构域、核苷酸结合结构域和富含亮氨酸重复结构域的蛋白质。
18.权利要求17的转基因植物或种子，其中所述卷曲螺旋结构域、所述核苷酸结合结构域和所述富含亮氨酸重复结构域的至少一种包含与SEQ ID NO:2或50中对应的结构域具有至少95％、96％、97％、98％、99％或100％的氨基酸序列。
19.权利要求17或18的转基因植物或种子，其中所述富含亮氨酸重复结构域包含选自以下的氨基酸：在对应于SEQ ID NO:2的氨基酸607的氨基酸位置处的丝氨酸(S)、在对应于SEQ ID NO:2的氨基酸650的氨基酸位置处的酪氨酸(Y)、在对应于SEQ ID NO:2的氨基酸651的氨基酸位置处的丝氨酸(S)，以及在对应于SEQ ID NO:2的氨基酸657的氨基酸位置处的甘氨酸(G)。
20.权利要求16-19中任意一项的转基因植物或种子，其中所述多核苷酸构建体包含(b)-(e)中任意一项的核苷酸序列并且进一步包含可操作地连接的用于在植物中表达所述核苷酸序列的启动子。
21.权利要求20的转基因植物或种子，其中所述启动子选自病原体诱导型、构成型、组织优选型、伤口诱导型和化学调节型启动子。
22.权利要求16-21中任意一项的转基因植物或种子，其中所述转基因植物为小麦植物并且所述转基因种子为小麦种子。
23.权利要求22的转基因植物或种子，其中相对于对照小麦植物而言，所述转基因植物或种子包含抵抗由小麦秆锈病菌的至少一个物种引起的小麦秆锈病的增强的抗性。
24.权利要求22或23的转基因植物或种子，其中所述多核苷酸构建体包含编码用于小麦秆锈病的R蛋白质的至少2个核苷酸序列。
25.权利要求24的转基因植物或种子，其中所述编码用于小麦秆锈病的R蛋白质的至少2个核苷酸序列的每一个编码用于小麦秆锈病的不同的R蛋白质。
26.用于增强小麦植物抵抗小麦秆锈病的抗性的方法，所述方法包括将多核苷酸构建体引入至少一种小麦植物细胞中，所述多核苷酸构建体包含选自以下的核苷酸序列：
(a)SEQ ID NO:1、4、7、10、19、22、25、34或49中列出的核苷酸序列；
(b)编码SEQ ID NO:2、5、8、11、20、23、26、35或50中列出的氨基酸序列的核苷酸序列，并且任选地，其中所述核苷酸序列不是天然形成的；
(c)SEQ ID NO:3、6、9、12、21、24、27、36或51中列出的核苷酸序列；
(d)与(a)或(c)中列出的核苷酸序列的至少一种具有至少85％序列一致性的核苷酸序列，其中所述核酸分子能够赋予包含所述核酸分子的小麦植物以抵抗秆锈病的抗性，并且任选地，其中所述核苷酸序列不是天然形成的；以及
(e)包含编码氨基酸序列的核苷酸序列的核酸分子，其中所述氨基酸序列与(c)中列出的至少一种氨基酸序列具有至少85％序列一致性，其中所述核酸分子能够赋予包含所述核酸分子的小麦植物以抵抗秆锈病的抗性，并且任选地，其中所述核苷酸序列不是天然形成的。
27.权利要求26的方法，其中(d)或(e)的所述核酸分子编码包含卷曲螺旋结构域、核苷酸结合结构域和富含亮氨酸重复结构域的蛋白质。
28.权利要求27的方法，其中所述卷曲螺旋结构域、所述核苷酸结合结构域和所述富含亮氨酸重复结构域的至少一种包含与SEQ ID NO:2或50中对应的结构域具有至少95％、96％、97％、98％、99％或100％的氨基酸序列。
29.权利要求27或28的方法，其中所述富含亮氨酸重复结构域包含选自以下的氨基酸：在对应于SEQ ID NO:2的氨基酸607的氨基酸位置处的丝氨酸(S)、在对应于SEQ ID NO:2的氨基酸650的氨基酸位置处的酪氨酸(Y)、在对应于SEQ ID NO:2的氨基酸651的氨基酸位置处的丝氨酸(S)，以及在对应于SEQ ID NO:2的氨基酸657的氨基酸位置处的甘氨酸(G)。
30.权利要求26-29中任意一项的方法，其中所述多核苷酸构建体被稳定地引入所述植物细胞的基因组中。
31.权利要求26-30中任意一项的方法，其中所述小麦植物细胞再生成在其基因组中包含所述多核苷酸构建体的小麦植物。
32.权利要求26-31中任意一项的方法，其中所述多核苷酸构建体包含(b)-(e)中任意一项的核苷酸序列并且进一步包含可操作地连接的用于在植物中表达所述核苷酸序列的启动子。
33.权利要求32的方法，其中所述启动子选自病原体诱导型、构成型、组织优选型、伤口诱导型和化学调节型启动子。
34.权利要求26-33中任意一项的方法，其中相对于对照小麦植物而言，包含所述多核苷酸构建体的所述小麦植物包含抵抗由小麦秆锈病菌的至少一个物种引起的小麦秆锈病的增强的抗性。
35.权利要求26-34中任意一项的方法，其中所述多核苷酸构建体包含编码用于小麦秆锈病的R蛋白质的至少2个核苷酸序列。
36.权利要求35的方法，其中所述编码用于小麦秆锈病的R蛋白质的至少2个核苷酸序列的每一个编码用于小麦秆锈病的不同的R蛋白质。
37.通过权利要求26-36中任意一项的方法产生的小麦植物。
38.权利要求37的小麦植物的种子，其中所述种子包含所述多核苷酸构建体。
39.在农业农作物生产中限制小麦秆锈病的方法，所述方法包括种植根据权利要求22-25和38中任意一项的小麦种子以及使小麦植物在有利于所述小麦植物生长和发育的条件下生长。
40.权利要求39的方法，其进一步包括由所述小麦植物收获至少一个种子。
41.权利要求22-25、37和38中任意一项的小麦植物或种子在农业中的用途。
42.使用权利要求22-25、37和38中任意一项的小麦植物或种子生产的人类或动物食物产品。
43.包含选自以下的氨基酸序列的多肽：
(a)由SEQ ID NO:1、4、7、10、19、22、25、34或49中列出的核苷酸序列编码的氨基酸序列；
(b)SEQ ID NO:2、5、8、11、20、23、26、35或50中列出的氨基酸序列；
(c)由SEQ ID NO:3中列出的核苷酸序列编码的氨基酸序列；以及
(d)与(c)中列出的至少一个氨基酸序列具有至少85％序列一致性的氨基酸序列，其中包含所述氨基酸序列的多肽能够赋予包含所述多肽的小麦植物以抵抗秆锈病的抗性，并且任选地，其中所述多肽不是天然形成的。
44.权利要求43的多肽，其中(d)的所述多肽包含卷曲螺旋结构域、核苷酸结合结构域和富含亮氨酸重复结构域。
45.权利要求44的多肽，其中所述卷曲螺旋结构域、所述核苷酸结合结构域和所述富含亮氨酸重复结构域的至少一种包含与SEQ ID NO:2或50中对应的结构域具有至少95％、96％、97％、98％、99％或100％的氨基酸序列。
46.权利要求44或45的多肽，其中所述富含亮氨酸重复结构域包含选自以下的氨基酸：在对应于SEQ ID NO:2的氨基酸607的氨基酸位置处的丝氨酸(S)、在对应于SEQ ID NO:2的氨基酸650的氨基酸位置处的酪氨酸(Y)、在对应于SEQ ID NO:2的氨基酸651的氨基酸位置处的丝氨酸(S)，以及在对应于SEQ ID NO:2的氨基酸657的氨基酸位置处的甘氨酸(G)。
47.用于鉴定小麦植物的方法，其中所述小麦植物展示新赋予的或增强的抵抗小麦秆锈病的抗性，所述方法包括检测所述小麦植物中选自Sr22和Sr45的R基因的存在情况。
48.权利要求47的方法，其中所述R基因的存在情况是通过检测所述R基因中至少一种标志物而检测的。
49.权利要求47或48的方法，其中所述方法包括检测Sr22和Sr45两者的存在情况。
50.权利要求47-49中任意一项的方法，其中所述Sr22包含SEQ ID NO:1、4、7、10、19、22、25或34中列出的核苷酸序列或由其组成，并且所述Sr45包含SEQ ID NO:49中列出的核苷酸序列或由其组成。
51.权利要求47-50中任意一项的方法，其中检测所述R基因的存在情况包括选自以下的一种：PCR扩增、核酸测序、核酸杂交以及用于检测由R基因编码的R蛋白质的免疫测试。
52.通过权利要求47-51中任意一项的方法鉴定的小麦植物。
53.权利要求52所述小麦植物的种子。
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