CN108014191A - A kind of Flos persicae extract and its application - Google Patents
A kind of Flos persicae extract and its application Download PDFInfo
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- CN108014191A CN108014191A CN201810098474.2A CN201810098474A CN108014191A CN 108014191 A CN108014191 A CN 108014191A CN 201810098474 A CN201810098474 A CN 201810098474A CN 108014191 A CN108014191 A CN 108014191A
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- China
- Prior art keywords
- flos persicae
- persicae extract
- hyaluronidase
- application
- extract
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- 239000000284 extract Substances 0.000 title claims abstract description 68
- 241000628997 Flos Species 0.000 title claims abstract description 65
- 108010003272 Hyaluronate lyase Proteins 0.000 claims abstract description 48
- 102000001974 Hyaluronidases Human genes 0.000 claims abstract description 47
- 229960002773 hyaluronidase Drugs 0.000 claims abstract description 47
- 235000006040 Prunus persica var persica Nutrition 0.000 claims abstract description 32
- 230000000694 effects Effects 0.000 claims abstract description 25
- 230000002401 inhibitory effect Effects 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 238000002360 preparation method Methods 0.000 claims abstract description 10
- 230000002829 reductive effect Effects 0.000 claims abstract description 7
- 238000002137 ultrasound extraction Methods 0.000 claims abstract description 7
- 239000002904 solvent Substances 0.000 claims abstract description 6
- 239000007864 aqueous solution Substances 0.000 claims abstract description 4
- 239000000243 solution Substances 0.000 claims description 29
- 244000144730 Amygdalus persica Species 0.000 claims description 28
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical group OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 9
- 230000003110 anti-inflammatory effect Effects 0.000 claims description 7
- 239000003814 drug Substances 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 5
- 229940122393 Hyaluronidase inhibitor Drugs 0.000 claims description 5
- 102100034343 Integrase Human genes 0.000 claims description 5
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims description 5
- 230000003266 anti-allergic effect Effects 0.000 claims description 5
- 239000012467 final product Substances 0.000 claims description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 4
- 230000036541 health Effects 0.000 claims description 4
- 239000003960 organic solvent Substances 0.000 claims description 4
- 239000000047 product Substances 0.000 claims description 4
- 235000013305 food Nutrition 0.000 claims description 3
- 230000001629 suppression Effects 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 abstract description 22
- 238000010521 absorption reaction Methods 0.000 abstract description 10
- 230000009471 action Effects 0.000 abstract description 3
- 230000001195 anabolic effect Effects 0.000 abstract description 2
- 238000009472 formulation Methods 0.000 abstract description 2
- 239000000203 mixture Substances 0.000 abstract description 2
- 239000012265 solid product Substances 0.000 abstract description 2
- 240000006413 Prunus persica var. persica Species 0.000 abstract 1
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 17
- 229920002674 hyaluronan Polymers 0.000 description 17
- 229960003160 hyaluronic acid Drugs 0.000 description 17
- 102000004190 Enzymes Human genes 0.000 description 10
- 108090000790 Enzymes Proteins 0.000 description 10
- 229940088598 enzyme Drugs 0.000 description 10
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 7
- 230000036632 reaction speed Effects 0.000 description 6
- BHZOKUMUHVTPBX-UHFFFAOYSA-M sodium acetic acid acetate Chemical compound [Na+].CC(O)=O.CC([O-])=O BHZOKUMUHVTPBX-UHFFFAOYSA-M 0.000 description 6
- 206010061218 Inflammation Diseases 0.000 description 5
- 229920002385 Sodium hyaluronate Polymers 0.000 description 5
- 230000008859 change Effects 0.000 description 5
- 230000004054 inflammatory process Effects 0.000 description 5
- 235000013944 peach juice Nutrition 0.000 description 5
- 239000007974 sodium acetate buffer Substances 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 239000003112 inhibitor Substances 0.000 description 4
- 229940010747 sodium hyaluronate Drugs 0.000 description 4
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 240000005809 Prunus persica Species 0.000 description 3
- 208000026935 allergic disease Diseases 0.000 description 3
- 230000003796 beauty Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 238000011161 development Methods 0.000 description 3
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- 235000011187 glycerol Nutrition 0.000 description 3
- 238000009413 insulation Methods 0.000 description 3
- 239000006210 lotion Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 2
- 206010020751 Hypersensitivity Diseases 0.000 description 2
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- 235000013334 alcoholic beverage Nutrition 0.000 description 2
- 125000005211 alkyl trimethyl ammonium group Chemical group 0.000 description 2
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- 238000010494 dissociation reaction Methods 0.000 description 2
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- 230000005764 inhibitory process Effects 0.000 description 2
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- 230000001151 other effect Effects 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000002798 spectrophotometry method Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 241001515965 unidentified phage Species 0.000 description 2
- 239000000341 volatile oil Substances 0.000 description 2
- ZGSCRDSBTNQPMS-UJURSFKZSA-N 3-O-Ethylascorbic acid Chemical group CCOC1=C(O)C(=O)O[C@@H]1[C@@H](O)CO ZGSCRDSBTNQPMS-UJURSFKZSA-N 0.000 description 1
- 235000019489 Almond oil Nutrition 0.000 description 1
- 241000282693 Cercopithecidae Species 0.000 description 1
- 206010010144 Completed suicide Diseases 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Natural products CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 102100039285 Hyaluronidase-2 Human genes 0.000 description 1
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- 201000009906 Meningitis Diseases 0.000 description 1
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- 230000033228 biological regulation Effects 0.000 description 1
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- CDQSJQSWAWPGKG-UHFFFAOYSA-N butane-1,1-diol Chemical compound CCCC(O)O CDQSJQSWAWPGKG-UHFFFAOYSA-N 0.000 description 1
- 229940105847 calamine Drugs 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
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- 230000001882 diuretic effect Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 235000018927 edible plant Nutrition 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
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- 235000013402 health food Nutrition 0.000 description 1
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- 238000009738 saturating Methods 0.000 description 1
- 239000002884 skin cream Substances 0.000 description 1
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 1
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
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- 201000004595 synovitis Diseases 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
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- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- -1 various preparations Substances 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
- 239000011787 zinc oxide Substances 0.000 description 1
- 235000014692 zinc oxide Nutrition 0.000 description 1
- CPYIZQLXMGRKSW-UHFFFAOYSA-N zinc;iron(3+);oxygen(2-) Chemical compound [O-2].[O-2].[O-2].[O-2].[Fe+3].[Fe+3].[Zn+2] CPYIZQLXMGRKSW-UHFFFAOYSA-N 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/736—Prunus, e.g. plum, cherry, peach, apricot or almond
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
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- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2800/74—Biological properties of particular ingredients
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- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
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Abstract
The present invention relates to a kind of Flos persicae extract and its application, application of more particularly to a kind of Flos persicae extract in hyaluronidase activity is suppressed.The Flos persicae extract is to utilize water or hydrophilic solvent and its aqueous solution, and ultrasonic extraction is carried out to peach blossom, and be concentrated under reduced pressure be evaporated after obtained light yellow or rose to wine-colored solid product, various preparations can be made, formulation uses.In order to verify action effect of the Flos persicae extract of the present invention to hyaluronidase activity, the present invention is by hyaluronidase and Flos persicae extract anabolic reaction system, absorption value of the reaction system in 400nm is measured, and inhibiting rate of the Flos persicae extract to hyaluronidase is calculated according to sample and the absorption value of control.The results show that Flos persicae extract produced by the present invention has obvious inhibitory action to hyaluronidase activity, and strengthen with the increase of application dose, there is preferable concentration dependant effect, its semi-inhibit dosage (IC to hyaluronidase activity50) it is 0.25mg/mL.
Description
Technical field
The present invention relates to a kind of Flos persicae extract and its application, more particularly to a kind of Flos persicae extract is suppressing hyaluronic acid
Application in enzymatic activity.
Background technology
Important component of the hyaluronic acid as extracellular matrix, except maintaining the integrality of extracellular net structure, sticking
Elasticity and playing adjusts Premeabilisation of cells pressure, connection cell, shielding, lubricating joint ease up outside the mechanical functions such as blow stress, also has
Adjust cell function, the physiological activity such as free radical are quenched, sent out in many developments of human body and regulation process such as cell adherence, form
Play an important role in life, wound healing, tumour generation, vascularization, pain and inflammation, allergy.Hyaluronidase
(Hyaluronidase, EC 3.2.1.35) is the Specific lytic enzymes of hyaluronic acid, can be saturating for oligomerization by hyaluronic acid degradation
Bright matter acid, so as to change the activity and function of hyaluronic acid.The synthesis of hyaluronic acid and by hyaluronic acid enzymatic degraded it
Between chronic unbalance cause joint disease and other types of inflammatory reaction.Intake external source Hyaluronidase inhibitor can hinder
Only in human body hyaluronic acid degraded, so as to prevent the occurrence and development for having related disorders with hyaluronic acid degradation.
With the high speed development of world industry, agricultural and science and technology, new technology, new material are constantly applied, along with environment
Deteriorate, inevitably resulted in various allergens so that allergic disease becomes a major class and threatens the mankind to be good for
The common disease and frequently-occurring disease of health.And pathological research then shows, to the inhibitory action and antiallergic action of hyaluronic acid enzyme activition
Between there is close correlation.Therefore, usually using the suppression of hyaluronidase activity experiment as basic skills, for from each
Allergy preparations is screened in kind material and compound.
In inflammatory reaction caused by germ infection, various bacteria, bacteriophage, fungi can express generation hyaluronidase,
Parasitize streptococcic bacteriophage and be attached to the bacterium film surface that hyaluronic acid enriches by synthesizing hyaluronidase.This is slightly
Biogenic hyaluronidase not only plays an important role in its pathogenic course, but also the hyaluronidase produced can drop
Solution hyaluronic acid and human body is susceptible to suffer from the inflammatory reactions such as meningitis, synovitis periodontal disease, pneumonia, and Hyaluronidase inhibitor
Can be by preventing the degraded of hyaluronic acid from alleviating these illnesss.
China's peach cultivated area and yield rank first in the world.The flower amount of peach is very big, about 9 times of requirement.In order to
Obtain higher yield and excellent fruit quality, every year in peach produces, to dredge it is late bloom, malformed flower, towards smallpox etc., dredge
/ 3rd of the about total flower amount of flower amount.Peach blossom have effects that beautifying face and moistering lotion, subtract the name of a river in Anhui Province make light of one's life by commiting suicide, swelling diuretic, toxin expelling freely just,
Ancient times are the beauty treatment weight reducing good merchantable brand for being used as imperial palace top grade.Peach blossom can be used to infusing drugs in wine, make tea, make health food, for beauty
Beauty treatment and weight-reducing, treatment constipation etc., and to the influence of peach blossom and its extract for hyaluronidase activity and its in anti-inflammatory and
Application in terms of antiallergic action, which has no, to be related to.
The content of the invention
In view of the deficiencies of the prior art, the object of the present invention is to provide a kind of Flos persicae extract and its application, the peach blossom to carry
Thing is taken to have the function that to suppress hyaluronidase activity.
To achieve these goals, the technical solution adopted in the present invention is：
A kind of Flos persicae extract, preparation method are：By peach blossom solvent using solid-to-liquid ratio as 1:5-10 (kg/L) is in room temperature bar
100-300W ultrasonic extractions 2 times under part, each 30-60min, filters and merges extracting solution, and extracting solution is dense in 40-45 DEG C of decompression
Contracting is evaporated, to obtain the final product.
The peach blossom is fresh peach blossom or bud, or, dries peach blossom or bud.
The solvent is water or hydrophilic organic solvent and its aqueous solution.
The hydrophilic organic solvent is methanol, ethanol, acetone.
A kind of application of Flos persicae extract in hyaluronidase activity is suppressed.
Flos persicae extract can suppress hyaluronidase activity, its semi-inhibit dosage IC50For 0.25mg/mL, suppress type
For linear hybrid type Reverse transcriptase, its inhibitory action has invertibity.
A kind of application of Flos persicae extract in Hyaluronidase inhibitor is prepared.
A kind of Flos persicae extract answering in the food of anti-inflammatory and antiallergic action, health products, cosmetics, medicine is prepared
With.
Beneficial effects of the present invention：
The Flos persicae extract of the present invention is to utilize water or hydrophilic solvent and its aqueous solution, and ultrasonic extraction is carried out to peach blossom,
And be concentrated under reduced pressure be evaporated after obtained light yellow or rose to wine-colored solid product, various preparations, formulation can be made
Use.In order to verify action effect of the Flos persicae extract of the present invention to hyaluronidase activity, the present invention by hyaluronidase and
Flos persicae extract anabolic reaction system, measures absorption value of the reaction system in 400nm, and according to sample and the absorption value meter of control
Calculate inhibiting rate of the Flos persicae extract to hyaluronidase.The results show that Flos persicae extract produced by the present invention is to hyaluronidase
Activity has obvious inhibitory action, and strengthens with the increase of application dose, has preferable concentration dependant effect, its is right
Semi-inhibit dosage (the IC of hyaluronidase activity50) it is 0.25mg/mL.
The present invention provides the new Hyaluronidase inhibitor and its system obtained in a kind of material from natural edible-plant
Preparation Method, to prepare anti-inflammatory and antiallergic action food, health products, cosmetics or medicine provide coming for new selection and safety
Source, also provides new application approach for peach blossom.Compared with existing medicine, Flos persicae extract is low with toxicity, securely and reliably, medicine
The characteristics of effect effect is strong, and aboundresources, technique is simple, and manufacturing cost is low, for improving resource utilization and increase peach plantation
Benefit has the function that positive, and economic benefit and social benefit are huge.
Brief description of the drawings
The embodiment of the present invention is described in further detail below in conjunction with attached drawing.
Fig. 1 is inhibition of the various concentrations Flos persicae extract to hyaluronidase activity.
Fig. 2 is Lineweaver-Burk curve of the various concentrations Flos persicae extract to hyaluronic acid enzyme inhibition.
Fig. 3 is Kmi of the various concentrations Flos persicae extract to hyaluronidase.
Fig. 4 is reaction speed V in the presence of Flos persicae extract to enzyme concentration ([E]) graph of a relation.
Embodiment
The embodiment of the present invention is described in further detail with reference to embodiments.
Embodiment 1
A kind of preparation method of Flos persicae extract：By peach blossom water using solid-to-liquid ratio as 1:5 (kg/L) are under normal temperature condition
100W ultrasonic extractions 2 times, each 30min, filter and merge extracting solution, and extracting solution is concentrated under reduced pressure at 45 DEG C and is evaporated, to obtain the final product.
Embodiment 2
A kind of preparation method of Flos persicae extract：By peach blossom methanol using solid-to-liquid ratio as 1:10 (kg/L) are under normal temperature condition
200W ultrasonic extractions 2 times, each 45min, filter and merge extracting solution, and extracting solution is concentrated under reduced pressure at 40 DEG C and is evaporated, to obtain the final product.
Embodiment 3
A kind of preparation method of Flos persicae extract：By peach blossom ethanol using solid-to-liquid ratio as 1:10 (kg/L) are under normal temperature condition
300W ultrasonic extractions 2 times, each 60min, filter and merge extracting solution, and extracting solution is concentrated under reduced pressure at 45 DEG C and is evaporated, to obtain the final product.
Measure of 4 Flos persicae extract of embodiment to hyaluronidase activity inhibitory action
With the 0.2mol/L acetic acid-sodium acetate buffer solutions (sodium chloride containing 0.15mol/L) of pH 6.0, compound concentration is respectively
The hyaluronidase solution and 0.344mg/mL sodium hyaluronate solutions of 1mg/mL, takes 45 μ L hyaluronidase solutions, 50 μ respectively
L sodium hyaluronate solutions and the mixing of different amounts of Flos persicae extract, then 0.5mL is supplied with acetic acid-sodium acetate buffer solution, form peach
The different reaction system of flower extract concentration；Reaction system is in 37 DEG C of water-bath insulation reaction 15min, with 2mL mass concentrations 1%0
Six alkyl trimethyl ammonium bromides (CTAB) solution terminate reaction, with spectrophotometric determination reaction system 400nm absorption value
A1, while measure and the reaction system of hyaluronidase is replaced in the absorption value A of 400nm with the hyaluronidase inactivated2.The same time-division
Flos persicae extract Ce Ding be not added with, only adds absorption of the reaction system of hyaluronidase or the hyaluronidase of inactivation in 400nm
Value A3And A4.Inhibiting rate is calculated as follows：
Inhibiting rate maps Flos persicae extract concentration in corresponding reaction system, the result is shown in Figure 1.Fig. 1 is the results show that originally
Flos persicae extract made from invention has hyaluronidase activity an obvious inhibitory action, and with application dose increase and
Enhancing, has preferable concentration dependant effect.Its semi-inhibit dosage (IC to hyaluronidase activity50) it is 0.25mg/mL.
5 Flos persicae extract of embodiment suppresses type to hyaluronidase and determines
With the 0.2mol/L acetic acid-sodium acetate buffer solutions (sodium chloride containing 0.15mol/L) of pH 6.0, compound concentration is respectively
The hyaluronidase solution and 0.344mg/mL sodium hyaluronate solutions of 1mg/mL, takes 25 μ L of hyaluronidase solution, peach respectively
Flower extract and sodium hyaluronate solution, then 0.5mL is supplied with acetic acid-sodium acetate buffer solution, measure peach blossom in reaction system and carry
When taking thing concentration different (0-0.3mg/mL) and the change of hyaluronic acid na concn (0.014-0.062mg/mL), reaction system exists
The light absorption value (method is with embodiment 4) of 400nm, while measure the reactant that hyaluronidase is replaced with the hyaluronidase inactivated
It is the absorption value of (control) in 400nm, according to sample with calculating enzyme's reaction speeding to the change for impinging upon A400nm, to react speed
Rate obtains Lineweaver-Burk curves (such as Fig. 2) to concentration of substrate with double-reciprocal plot, and calculates the power of enzymic catalytic reaction
Parameter Kmi and Vmax value is learned, judges Flos persicae extract to hyaluronidase activity by comparing the change of Kmi and Vmax values
Suppress type.Fig. 2 the result shows that, enzyme kinetics curve when adding Flos persicae extract and dynamics during no inhibitor are bent
Line intersects at the second quadrant, adds the maximum reaction velocity Vmax reductions of enzymatic reaction after Flos persicae extract, Michaelis constant Kmi liters
Height, and the dissociation constant KI of the dissociation constant KSI ＞ inhibitor of substrate suppression.Therefore, Flos persicae extract is to hyaluronidase
Mixed type Reverse transcriptase.
Kmi (Michaelis constant during Reverse transcriptase) of the 6 various concentrations Flos persicae extract of embodiment to hyaluronidase
Figure is obtained by concentration ([I]) mappings of the Kmi under various concentrations Flos persicae extract existence condition to Flos persicae extract
3, Fig. 3 the result shows that, with the increase of Flos persicae extract concentration in reaction system, Kmi increases, the i.e. binding ability of enzyme-to-substrate
Decline, activated complex is reduced, so that reaction rate declines.In addition from figure 3, it can be seen that Kmi and Flos persicae extract concentration
Relation between ([I]) is a straight line, therefore Flos persicae extract is linear hybrid type Reverse transcriptase to hyaluronidase.
By 1mg/mL hyaluronidase solutions and 5mg/mL Flos persicae extracts acetic acid-sodium acetate solution with volume ratio 9:10
Ratio mixes, and after 37 DEG C of insulation 15min, takes different volumes (5-150 μ L) mixed liquor to add 40 μ L 3.44mg/mL Sodium Hyaluronates
Solution, then adds acetic acid-sodium acetate buffer solution to 0.5mL, 37 DEG C of water-bath insulation reaction 15min, with 2mL mass concentrations 1%0
Six alkyl trimethyl ammonium bromides (CTAB) solution terminate reaction, with spectrophotometric determination reaction system 400nm absorption value.
The system for being not added with Flos persicae extract is set at the same time as no inhibitor group.Measure is replaced transparent with the hyaluronidase inactivated at the same time
The reaction system (control) of matter acid enzyme 400nm absorption value, by sample with to impinge upon A400nm change calculate enzymatic reaction
Speed, Fig. 4 is obtained with reaction speed V to enzyme concentration ([E]) mapping.As seen from Figure 4, there is Flos persicae extract existence condition
Under, with the increase of enzyme concentration, reaction speed increase, and finally enter a plateau.Though there is the substrate of same concentrations,
During identical enzyme concentration, reaction speed is below reaction speed during no inhibitor.Therefore, Flos persicae extract is to hyaluronidase
Reversible inhibition.
7 peach juice low alcohol beverage of embodiment
The Flos persicae extract of peach juice mass fraction 0.3% is added in peach juice, is inoculated with the saccharomyces cerevisiae after spreading cultivation, inoculum concentration
17-22 DEG C of 1-3%, fermentation temperature, fermentation are filtered after a week, and karusen is made.By weight percentage, according to karusen
40%th, peach juice 20%, white granulated sugar 10%, citric acid 0.2%-0.5%, flavoring peach essence 0.02%-0.1%, remaining is water, through adjusting
Match somebody with somebody, homogeneous, sterilization, it is filling be made peach juice low alcohol beverage, auxiliary is used to improve inflammatory reaction.
8 peach blossom lotion of embodiment/frost
(1) peach blossom lotion：By weight percentage, including Flos persicae extract 0.01-1%, hyaluronic acid 0.1-1%,
Peach blossom essential oil 0.1-0.5%, glycerine 1-3%, butanediol 2-5%, remaining is water.With anti-inflammatory, whitening, moisturizing and other effects.
(2) peach blossom skin cream：By weight percentage, including Flos persicae extract 0.01-2%, Sweet Almond Oil 5-10%, Mi
Monkey peach seed oil 5-10%, peach blossom essential oil 0.1-0.5%, hyaluronic acid 0.1-0.5%, Vitamin C Ethyl Ether 0.5-2.0%, table
Face activating agent G57 0.5-2.0%, remaining is water.With anti-oxidant, light spot, nourishes rough skin and anti-inflammatory and other effects.
External preparation for skin medicine of the embodiment 9 containing Flos persicae extract
By weight percentage, including calamine 15%, Flos persicae extract 3%, glycerine 5%, remaining is water.Stove is sweet
Stone, Flos persicae extract are uniformly mixed after being ground into fine powder, and glycerol adding, a small amount of water are ground into paste, then add full institute's water requirement, are made mixed
Suspension；Add weight of suspension 0.2-1% sodium carboxymethylcelluloses, stirred evenly after being sufficiently stirred dissolving.It is used for skin table outside
Face is embrocated, for skin anti-inflammatory, itching-relieving action.
The foregoing is merely the embodiment that the present invention is optimal, for those skilled in the art, the present invention can have
Various modifications and variations.Within the spirit and principles of the invention, any modification, equivalent replacement, improvement and so on, should all
Within protection scope of the present invention.
Claims (8)
1. a kind of Flos persicae extract, it is characterised in that the preparation method of the Flos persicae extract is：By peach blossom solvent with solid-liquid
Than for 1:5-10 (kg/L) 100-300W ultrasonic extractions 2 times under normal temperature condition, each 30-60min, filters and merges extraction
Liquid, extracting solution is concentrated under reduced pressure at 40-45 DEG C and is evaporated, to obtain the final product.
2. Flos persicae extract according to claim 1, it is characterised in that the peach blossom is fresh peach blossom or bud, or,
Dry peach blossom or bud.
3. Flos persicae extract according to claim 1, it is characterised in that the solvent is water or hydrophilic organic solvent
And its aqueous solution.
4. Flos persicae extract according to claim 3, it is characterised in that the hydrophilic organic solvent is methanol, second
Alcohol, acetone.
A kind of 5. application of the Flos persicae extract in hyaluronidase activity is suppressed as claimed in claim 1.
6. application according to claim 5, it is characterised in that Flos persicae extract can suppress hyaluronidase activity, its
Semi-inhibit dosage IC50For 0.25mg/mL, suppression type is linear hybrid type Reverse transcriptase, its inhibitory action has invertibity.
A kind of 7. application of the Flos persicae extract in Hyaluronidase inhibitor is prepared as claimed in claim 1.
8. a kind of Flos persicae extract as claimed in claim 1 is preparing the food, health products, makeup of anti-inflammatory and antiallergic action
Application in product, medicine.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108704081A (en) * | 2018-08-15 | 2018-10-26 | 佛山汇沐化学科技有限公司 | A kind of beautifying face and moistering lotion lavipeditum powder and preparation method thereof |
| CN108785243A (en) * | 2018-08-15 | 2018-11-13 | 李媛媛 | A kind of anti-aging whitening acne eliminating cream and preparation method thereof |
| CN111870558A (en) * | 2020-08-17 | 2020-11-03 | 山东华熙海御生物医药有限公司 | Composition for inhibiting hyaluronidase activity and skin care product |
| CN114848548A (en) * | 2022-04-25 | 2022-08-05 | 李利 | Peach blossom extract-fullerene compound and preparation method and application thereof |
| CN116870061A (en) * | 2023-08-04 | 2023-10-13 | 无限极（中国）有限公司 | Peach blossom extract and preparation method and application thereof |
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2018
- 2018-01-31 CN CN201810098474.2A patent/CN108014191A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108704081A (en) * | 2018-08-15 | 2018-10-26 | 佛山汇沐化学科技有限公司 | A kind of beautifying face and moistering lotion lavipeditum powder and preparation method thereof |
| CN108785243A (en) * | 2018-08-15 | 2018-11-13 | 李媛媛 | A kind of anti-aging whitening acne eliminating cream and preparation method thereof |
| CN108785243B (en) * | 2018-08-15 | 2021-04-20 | 广州惜颜生物科技有限公司 | Anti-aging whitening acne-removing cream and preparation method thereof |
| CN111870558A (en) * | 2020-08-17 | 2020-11-03 | 山东华熙海御生物医药有限公司 | Composition for inhibiting hyaluronidase activity and skin care product |
| CN114848548A (en) * | 2022-04-25 | 2022-08-05 | 李利 | Peach blossom extract-fullerene compound and preparation method and application thereof |
| CN114848548B (en) * | 2022-04-25 | 2024-02-13 | 李利 | Peach blossom extract-fullerene compound and preparation method and application thereof |
| CN116870061A (en) * | 2023-08-04 | 2023-10-13 | 无限极（中国）有限公司 | Peach blossom extract and preparation method and application thereof |
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Application publication date: 20180511 |