CN108012994B - Collecting method for oral secretion of small lepidoptera larva - Google Patents
Collecting method for oral secretion of small lepidoptera larva Download PDFInfo
- Publication number
- CN108012994B CN108012994B CN201711268737.1A CN201711268737A CN108012994B CN 108012994 B CN108012994 B CN 108012994B CN 201711268737 A CN201711268737 A CN 201711268737A CN 108012994 B CN108012994 B CN 108012994B
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- Prior art keywords
- small
- oral secretion
- lepidoptera
- larva
- adopts
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- 230000028327 secretion Effects 0.000 title claims abstract description 37
- 241000255777 Lepidoptera Species 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 19
- 238000007664 blowing Methods 0.000 claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 10
- 238000012546 transfer Methods 0.000 claims abstract description 10
- 238000005096 rolling process Methods 0.000 claims abstract description 7
- 241000238631 Hexapoda Species 0.000 claims description 8
- 239000003566 sealing material Substances 0.000 claims description 5
- 241000500437 Plutella xylostella Species 0.000 claims description 3
- 239000002390 adhesive tape Substances 0.000 claims description 3
- 238000005452 bending Methods 0.000 claims description 2
- 239000003755 preservative agent Substances 0.000 claims description 2
- 230000002335 preservative effect Effects 0.000 claims description 2
- 239000000463 material Substances 0.000 abstract description 3
- 238000010586 diagram Methods 0.000 description 3
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 241000255789 Bombyx mori Species 0.000 description 1
- 206010067171 Regurgitation Diseases 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 230000004665 defense response Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 239000005712 elicitor Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention relates to a method for collecting oral secretion of small lepidoptera larvae, which is characterized by comprising the following steps of: (1) rolling the middle-front part of the small lepidoptera larva from back to front by using a roller type pressing device; (2) collecting oral secretion by a liquid transfer device when the small lepidoptera larva spits out the oral secretion; (3) and finally, blowing the oral secretion in the pipettor into the collection container by using a blowing device. The method is easy to operate, easy in material obtaining, low in cost and high in collection efficiency, can be used for collecting oral secretion of the small lepidoptera larva by one person, and has wide application prospect.
Description
Technical Field
The invention relates to a method for collecting oral secretion of small lepidoptera larvae.
Background
Oral secretions of lepidopteran insects are also called regurgitation, and often contain various components such as insect saliva and digestive juice of the midgut. When lepidopteran larvae feed on plants, elicitors in oral secretions can elicit a defense response in the plant. The mutual relationship between insects and plants needs to be deeply researched, the action mechanism of insect induced plant defense reaction is clarified, and the acquisition of oral secretion of lepidoptera larvae is an important precondition.
The method for obtaining oral secretion of lepidoptera insect larvae mainly comprises the following steps of (1) pressing larva bodies to obtain oral secretion; (2) freezing the larva for several minutes, and then placing the larva at normal temperature until the larva recovers the secretion of the spitting cavity; (3) the head of the insect is collided to cause the secretion of the cavity of the outlet of the insect. In the above methods, only oral secretions from larger lepidopteran larvae are typically collected, for example: and Bombyx mori and the like. But for smaller lepidopteran larvae, for example: the above methods cannot obtain a large amount of oral secretion from diamondback moth. At present, a method for collecting the small lepidoptera larvae is lacked, so that the research on the action mechanism of the interrelation between the small lepidoptera pests and plants is slow. Therefore, it is necessary to find a method for collecting oral secretion of small lepidopteran larvae.
Disclosure of Invention
In view of the defects of the prior art, the invention aims to provide a method for collecting oral secretion of small lepidoptera larvae, which is reasonable in structural design, efficient and convenient.
In order to solve the technical problems, the technical scheme of the invention is as follows: a collection method of oral secretion of small lepidoptera larvae comprises the following steps:
(1) rolling the middle-front part of the small lepidoptera larva from back to front by using a roller type pressing device;
(2) collecting oral secretion by a liquid transfer device when the small lepidoptera larva spits out the oral secretion;
(3) and finally, blowing the oral secretion in the pipettor into the collection container by using a blowing device.
Preferably, the roller type pressing device comprises a handle, a connecting shaft is arranged at the front end of the handle, and a pipe body for rolling is sleeved outside the connecting shaft.
Preferably, the handle is Y-shaped.
Preferably, the rear end of the pipettor is provided with a receiver for butting a blowing device, the receiver is funnel-shaped, and the pipettor and the receiver are sealed by a sealing material.
Preferably, the pipette uses a capillary tube.
Preferably, the blowing device adopts an ear suction ball.
Compared with the prior art, the invention has the following beneficial effects: the method is easy to operate, easy in material obtaining, low in cost and high in collection efficiency, can be used for collecting oral secretion of the small lepidoptera larva by one person, and has wide application prospect.
The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.
Drawings
FIG. 1 is a schematic configuration diagram of an embodiment of the present invention.
FIG. 2 is a schematic configuration diagram of an embodiment of the present invention.
FIG. 3 is a schematic configuration diagram of an embodiment of the present invention.
In the figure: 1-roller type pressing device, 101-handle, 102-connecting shaft, 103-tube, 2-small lepidoptera larva, 3-pipettor, 4-oral secretion, 5-blowing device, 6-collecting container and 7-receiver.
Detailed Description
In order to make the aforementioned and other features and advantages of the invention more comprehensible, embodiments accompanied with figures are described in detail below.
As shown in figures 1-3, a method for collecting oral secretion of small lepidoptera larva comprises the following steps:
(1) rolling the middle front part of the small lepidoptera larva 2 from back to front by using the roller type pressing device 1;
(2) when the small lepidoptera larva 2 spits out the oral secretion 4, collecting the oral secretion 4 by using a liquid transfer device 3;
(3) finally, the oral secretion 4 in the pipettor 3 is blown into the collection container 6 by the blowing device 5.
In the embodiment of the present invention, the roller pressing device 1 includes a handle 101, a connecting shaft 102 is disposed at a front end of the handle 101, and a tube 103 for rolling is sleeved outside the connecting shaft 102; preferably, an adhesive tape is wound on the outside of the tube 103, and the diameter of the tube 103 is controlled by controlling the number of turns of the wound adhesive tape; preferably, the width of the tubular body 103 is greater than or equal to the width of the small lepidopteran larva 2; preferably, the handle 101 can be formed by bending a clip, the connecting shaft 102 can be an insect clip, and the tube body 103 can be a capillary tube, so that the material is easy to obtain and the cost is low; roller type press device 1 can avoid causing the injury to small-size lepidoptera larva 2, improves the survival rate of small-size lepidoptera larva 2 in the in-process of oral secretion 4 collection.
In the present embodiment, the handle 101 is in a Y shape.
In the embodiment of the invention, the rear end of the liquid transfer device 3 is provided with a receiver 7 for butting the blowing device 5, the receiver 7 is funnel-shaped, and the liquid transfer device 3 and the receiver 7 are sealed by a sealing material; the receiver 7 can adopt a gun head of a liquid-transfering gun; the sealing material can be preservative film.
In the present embodiment, the pipette 3 is a capillary tube.
In the embodiment of the present invention, the blowing device 5 is an ear-sucking ball.
In the embodiment of the invention, 60 plutella xylostella 4-instar larvae are taken, 3 groups are divided into 3 groups, each group has 20 heads, oral secretion 4 is collected, and each larva is pressed for 3 times by the method; the results of the experiment are shown in table 1.
The present invention is not limited to the above-described preferred embodiments, and various other types of collection methods of oral secretion from lepidopteran larvae can be developed by anyone based on the teaching of the present invention. All equivalent changes and modifications made according to the claims of the present invention should be covered by the present invention.
Claims (1)
1. A collection method of oral secretion of small lepidoptera larvae is characterized by comprising the following steps:
(1) rolling the middle-front part of the small lepidoptera larva from back to front by using a roller type pressing device;
(2) collecting oral secretion by a liquid transfer device when the small lepidoptera larva spits out the oral secretion;
(3) finally, blowing the oral secretion in the pipettor into a collection container by using a blowing device;
the roller type pressing device comprises a handle, a connecting shaft is arranged at the front end of the handle, a pipe body used for rolling is sleeved outside the connecting shaft, the handle is Y-shaped, a receiver used for being abutted to a blowing device is arranged at the rear end of the liquid transfer device, the receiver is funnel-shaped, the liquid transfer device and the receiver are sealed through a sealing material, the liquid transfer device adopts a capillary tube, the blowing device adopts an ear suction ball, an adhesive tape is wound outside the pipe body, the width of the pipe body is larger than or equal to that of small lepidoptera larvae, the handle is formed by bending a clip, the connecting shaft adopts an insect needle, the pipe body adopts a capillary tube, the receiver adopts a gun head of a liquid transfer gun, and the sealing material adopts a preservative film; collecting oral secretion of diamondback moth 4-instar larvae of 60 heads, dividing into 3 groups, each group has 20 heads, and pressing each larva for 3 times by the above method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711268737.1A CN108012994B (en) | 2017-12-05 | 2017-12-05 | Collecting method for oral secretion of small lepidoptera larva |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711268737.1A CN108012994B (en) | 2017-12-05 | 2017-12-05 | Collecting method for oral secretion of small lepidoptera larva |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108012994A CN108012994A (en) | 2018-05-11 |
| CN108012994B true CN108012994B (en) | 2020-02-14 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711268737.1A Expired - Fee Related CN108012994B (en) | 2017-12-05 | 2017-12-05 | Collecting method for oral secretion of small lepidoptera larva |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108012994B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111103171B (en) * | 2019-12-30 | 2021-07-30 | 中南大学 | Cryptopteris macrocephala secretion collecting device and method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2170961T3 (en) * | 1996-08-19 | 2002-08-16 | Us Agriculture | ARTIFICIAL MEANS TO CREATE ENTOMOFAGOS. |
| CN201978755U (en) * | 2010-12-20 | 2011-09-21 | 金华职业技术学院 | Rubber head sucker with pipettor tip |
| CN202468241U (en) * | 2012-02-09 | 2012-10-03 | 南京中竞科电子科技有限公司 | Micro peristaltic pump |
| CN205623973U (en) * | 2016-05-12 | 2016-10-12 | 中国农业科学院棉花研究所 | Piercingsucking mouthparts insect oral secretion's collection device |
-
2017
- 2017-12-05 CN CN201711268737.1A patent/CN108012994B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2170961T3 (en) * | 1996-08-19 | 2002-08-16 | Us Agriculture | ARTIFICIAL MEANS TO CREATE ENTOMOFAGOS. |
| CN201978755U (en) * | 2010-12-20 | 2011-09-21 | 金华职业技术学院 | Rubber head sucker with pipettor tip |
| CN202468241U (en) * | 2012-02-09 | 2012-10-03 | 南京中竞科电子科技有限公司 | Micro peristaltic pump |
| CN205623973U (en) * | 2016-05-12 | 2016-10-12 | 中国农业科学院棉花研究所 | Piercingsucking mouthparts insect oral secretion's collection device |
Non-Patent Citations (1)
| Title |
|---|
| 总结与讨论;黄宇萍;《小菜蛾诱导的甘蓝挥发性物质及其效应"，黄宇萍，中国优秀硕士学位论文全文数据库 农业科技辑》;20121215(第12期);第45-46页 * |
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| Publication number | Publication date |
|---|---|
| CN108012994A (en) | 2018-05-11 |
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