CN108004117B

CN108004117B - Microalgae culture device

Info

Publication number: CN108004117B
Application number: CN201711249311.1A
Authority: CN
Inventors: 胡壮涛; 丁玉龙; 王广宁; 葛立保; 马家好
Original assignee: Fujian Dongfang Liyang Seed Breeding Co ltd
Current assignee: Fujian Dongfang Liyang Seed Breeding Co ltd
Priority date: 2017-12-01
Filing date: 2017-12-01
Publication date: 2021-02-19
Anticipated expiration: 2037-12-01
Also published as: CN108004117A

Abstract

Disclosed is a microalgae culture device, which comprises a cuboid culture main body (1) and a cuboid box (2) arranged inside the culture main body (1), wherein the cuboid box (2) and the culture main body (1) share a top wall (3), the top wall (3) of the culture main body (1) is provided with a first round hole (4) and a second round hole (5), a bottom wall (6) of the culture main body (1) is provided with a roughly annular nanotube (7) around the cuboid box (2), a first pipe (8) inserted into the first round hole (4) is connected with an upper port of a reducing tee (9), a left port and a right port of the reducing tee (9) are communicated with the nanotube (7), a second pipe (10) inserted into the second round hole (5) is fixed on a side wall of the culture main body (1), the nanotube (7) and the second pipe (10) are provided with a plurality of micropores, the inner wall of the cuboid box (2) is provided with at least one LED lamp tube (11).

Description

Microalgae culture device

Technical Field

The invention relates to the field of microalgae, in particular to a microalgae culture device.

Background

Microalgae are groups of algae that are small in size, intermediate between microorganisms and plants, and capable of oxygen-releasing photosynthesis. Microalgae cells can effectively utilize light energy through photosynthesis to convert H2O, CO2 and inorganic salts into organic substances and release oxygen, thereby creating conditions for the survival of other organisms. In addition, the microalgae cells are rich in various high-value-added biological substances such as proteins, amino acids, carbohydrates, vitamins, antibiotics, highly unsaturated fatty acids, polysaccharides, pigments and the like, and are important resources of human food, medicines, fuels, fine chemical materials and the like.

In the aquatic breeding industry, plant baits (microalgae) and animal baits (rotifers, fairy worms and the like) are mainly used in the process of breeding shrimp, crab and fish fries, wherein the baits used by the animal baits are also the microalgae. The microalgae used as a primary producer is an indispensable important bait in the process of culturing the aquatic animal seedlings. Especially, the unsaturated fatty acid content in the microalgae is high, so that enough nutrition is provided for the early stage of the seedling. Some microalgae can also produce pigments such as astaxanthin and the like, and can improve the immunity and the anti-stress capability of seedlings.

The high-efficiency culture of the microalgae cells is a key link for developing and utilizing microalgae resources. At present, microalgae mainly adopts a photoautotrophic culture mode, and has several problems in the existing expanded culture: 1. the intensity of the light is not suitable. Light intensity is a major factor affecting photosynthesis and cultivation efficiency of microalgae. Insufficient or excessive light intensity or uneven light transmission is a major problem in the expanded culture. 2. Microalgae adhere to the wall and sink to the bottom in a large amount. Microalgae are prone to adherent growth. When the water body is unevenly stirred and a large amount of microalgae adhere to the wall and sink to the bottom, the effect of shading is achieved, the utilization of light and nutrient substances cannot be achieved, and the death of the microalgae is caused. 3. Is easy to be polluted by the outside. The open culture device allows exogenous bacteria, fungi, protozoa or other microalgae to enter the culture device, causing pollution. 4. The structure is not compact, the cleaning is difficult, and the secondary pollution is easy. In the existing tubular incubator, in order to achieve the maximum utilization of a light source, a tube is slender and occupies a large space; meanwhile, in order to enhance the sealing performance, the cleaning is not easy. How to realize the high-efficiency culture of microalgae is an important direction for the research of microalgae at present.

Disclosure of Invention

Problems to be solved by the invention

The invention aims to provide a device for microalgae enlarged culture, which has a compact structure, can improve the unit illumination area, is not easy to cause exogenous pollution, can control the adherent growth phenomenon of microalgae, is easy to clean, can achieve higher microalgae yield and realizes high-efficiency culture of the microalgae. The invention has reasonable design, convenience and practicability.

Means for solving the problems

The present inventors have conducted intensive studies to achieve the above objects, and specifically, a microalgae culture apparatus includes a rectangular culture body and a rectangular box disposed inside the culture body, the rectangular box and the culture body share a top wall, the top wall of the culture body is provided with a first circular hole and a second circular hole, the bottom wall of the culture body is surrounded by a substantially annular nanotube, the first tube inserted into the first circular hole is connected to an upper opening of a reducing tee, a left opening and a right opening of the reducing tee are communicated with the nanotube, the second tube inserted into the second circular hole is fixed on a side wall of the culture body, the nanotube and the second tube are provided with a plurality of micropores, wherein the density of the micropores of the nanotube is 700-, one end of the elbow is connected with the third round hole.

In the microalgae culturing device, the top wall is provided with a handle, and the bottom wall is provided with a column foot for supporting.

In the microalgae culturing apparatus, the culturing main body and the cuboid box are made of an acrylic material, and the first pipe and the second pipe are made of a PVC material.

In the microalgae culturing device, the culture main body is 100cm long, 40cm wide, 80cm high and 1cm thick.

In the microalgae culturing device, the first round hole is 5cm away from the rear wall of the culturing main body and 50cm away from the side wall of the culturing main body, the diameter of the first round hole is 3cm, the diameter of the second round hole is 3cm, and the diameter of the third round hole is 3.5 cm.

In the microalgae culture device, the cuboid box is 30cm long, 20cm wide, 80cm high and 0.8cm thick.

In the microalgae culturing device, the distance between the left side wall of the cuboid box and the left side wall of the culturing main body is 35cm, and the distance between the front wall of the cuboid box and the front wall of the culturing main body is 10 cm.

In the microalgae culture device, the diameter of the first pipe and/or the second pipe is 2.5cm, the second pipe is adhered to the side wall of the culture main body, and the diameter of the elbow is 3.5 cm.

In the microalgae culture device, the power of the LED lamp tube is 25 watts, the color temperature is 6500K, the length of the LED lamp tube is 75cm, and the diameter is 2.5 cm.

In the microalgae culture device, the LED lamp tube is vertically placed on the bottom wall of the cuboid box, wherein the distance between the LED lamp tube and the side wall of the cuboid box is 10 cm.

Compared with the prior art, the invention has the following advantages: (1) the unit illumination surface area is large. The microalgae cells can fully absorb light, enhance photosynthesis and accelerate propagation. (2) The micro-algae has strong fluidity. The water flow is clockwise internal circulation under the guidance of the multi-section inflation, the flow of the microalgae cells is driven, no dead angle exists, the microalgae cells cannot sink to the bottom and adhere to the wall, and the phenomenon of adherent growth of the microalgae is controllable. (3) Easy to operate and clean. The short culture device is convenient for operators to feed water and drain water, collects the algae liquid, is beneficial to cleaning, and does not cause secondary pollution. (4) The structure is compact. The culture device has a small and compact structure and can better utilize the space. Can be placed in the same space in a large scale and can be produced. (5) Is not easy to cause the pollution of the external bacteria. The closed culture device can reduce the pollution of exogenous bacteria, fungi, protozoa, other algae and impurities and maintain the high-density culture of algae cells at high purity. (6) Higher microalgae yield. By improving the light receiving area of microalgae, the photosynthesis of microalgae cells is enhanced, the proliferation of the microalgae cells is accelerated, and meanwhile, the water body circularly flows in the device in a clockwise manner, so that the absorption of the microalgae cells on carbon dioxide gas, light and nutrient substances is increased, and the yield of the microalgae is improved. The device for the expanded culture of the microalgae is ingenious in design, excellent in performance, convenient and practical.

The above description is only an overview of the technical solutions of the present invention, and in order to make the technical means of the present invention more clearly apparent, and to make the implementation of the content of the description possible for those skilled in the art, and to make the above and other objects, features and advantages of the present invention more obvious, the following description is given by way of example of the specific embodiments of the present invention.

Drawings

FIG. 1 is a schematic perspective view showing a microalgae cultivation apparatus according to an embodiment of the present invention.

Detailed Description

Specific embodiments of the present invention will be described in more detail below with reference to the accompanying drawings. While specific embodiments of the invention are shown in the drawings, it should be understood that the invention may be embodied in various forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.

It should be noted that certain terms are used throughout the description and claims to refer to particular components. As one skilled in the art will appreciate, various names may be used to refer to a component. This specification and claims do not intend to distinguish between components that differ in name but not function. In the following description and in the claims, the terms "include" and "comprise" are used in an open-ended fashion, and thus should be interpreted to mean "include, but not limited to. The description which follows is a preferred embodiment of the invention, but is made for the purpose of illustrating the general principles of the invention and not for the purpose of limiting the scope of the invention. The scope of the present invention is defined by the appended claims.

For the purpose of facilitating an understanding of the embodiments of the present invention, the following description will be made in terms of several specific embodiments with reference to the accompanying drawings, and the drawings are not intended to limit the embodiments of the present invention.

Specifically, as shown in fig. 1, which is a schematic perspective view of a microalgae culture apparatus in one embodiment of the present invention, the microalgae culture apparatus includes a cuboid culture body 1 and a cuboid box 2 disposed inside the culture body 1, the cuboid box 2 and the culture body 1 share a top wall 3, the top wall 3 of the culture body 1 is provided with a first circular hole 4 and a second circular hole 5, a substantially annular nanotube 7 is disposed around the cuboid box 2 on a bottom wall 6 of the culture body 1, a first tube 8 inserted into the first circular hole 4 is connected with an upper port of a reducer tee 9, a left port and a right port of the reducer tee 9 are communicated with the nanotube 7, a second tube 10 inserted into the second circular hole 5 is fixed on a side wall of the culture body 1, the nanotube 7 and the second tube 10 are provided with a plurality of micropores, wherein the density of the nanotube 7 is 700-, the inner wall of the cuboid box 2 is provided with at least one LED lamp tube 11, the bottom wall 6 of the culture main body 1 is provided with a third round hole 12, and one end of the elbow 13 is connected with the third round hole 12.

The culture device in the prior art has small unit illumination area, and microalgae cells only have illumination from the top and cannot sufficiently absorb light, so that the effect of rapid propagation is achieved. The culture apparatus in the prior art has the advantages that only up-and-down convection is carried out on water flow, the water flow cannot form an effective circulation loop, dead angles exist between microalgae cells at the bottom of a barrel and the edge of the barrel easily, a large amount of adherent growth of the microalgae cells is caused, sufficient CO2 and nutrients cannot be absorbed, meanwhile, the microalgae cells cannot be irradiated only on the surface of a water body along with the water flow, and along with the death of part of the microalgae cells, the bred bacteria and protozoa also accelerate the aging of other microalgae cells, so that the microalgae cultivation fails. The existing culture device has good sealing performance, but has a huge structure, the height of a tube-type culture device with the diameter of 40cm is 2 meters, the length of a thin-tube-type culture device with the diameter of 30cm is more than 10 meters, the huge structure causes small space utilization rate, the production operation is difficult, the cleaning is not easy, and the secondary pollution is easily caused.

The microalgae culture device provided by the invention has the advantages that the yield and the quality of microalgae are obviously improved. The concave inner structure of the microalgae culture device is ingenious in inflation mode, microalgae cells can be driven to flow and circulate along with a water body, meanwhile, light is arranged in the device, the light receiving surface area of the microalgae is enlarged, the culture device has no dead angle, the microalgae cells cannot sink to the bottom and adhere to the wall, and the phenomenon of adherent growth of the microalgae is controllable. The short culture device is convenient for operators to feed water and drain water, collects the algae liquid, is beneficial to cleaning, and does not cause secondary pollution. The culture device has a small and compact structure and can better utilize the space. Meanwhile, the absorption of carbon dioxide gas, light and nutrient substances by microalgae cells is increased, and the yield of microalgae is improved.

In the preferred embodiment of the microalgae cultivation apparatus of the present invention, the top wall 3 is provided with a handle 14, and the bottom wall 6 is provided with a support pedestal 15.

In a preferred embodiment of the microalgae cultivation apparatus of the present invention, the cultivation body 1 and the rectangular parallelepiped box 2 are made of an acryl material, and the first tube 8 and the second tube 10 are made of a PVC material.

In a preferred embodiment of the microalgae cultivation apparatus of the present invention, the cultivation body 1 has a length of 100cm, a width of 40cm, a height of 80cm and a thickness of 1 cm.

In a preferred embodiment of the microalgae cultivation apparatus of the present invention, the first circular hole 4 is 5cm from the rear wall of the cultivation body 1 and 50cm from the side wall of the cultivation body 1, the diameter of the first circular hole 4 is 3cm, the diameter of the second circular hole 5 is 3cm, and the diameter of the third circular hole 12 is 3.5 cm.

In a preferred embodiment of the microalgae cultivation apparatus of the present invention, the rectangular parallelepiped box 2 has a length of 30cm, a width of 20cm, a height of 80cm and a thickness of 0.8 cm.

In the preferred embodiment of the microalgae cultivation apparatus of the present invention, the left side wall of the rectangular parallelepiped box 2 is 35cm away from the left side wall of the cultivation main body 1, and the front wall of the rectangular parallelepiped box 2 is 10cm away from the front wall of the cultivation main body 1.

In a preferred embodiment of the microalgae cultivation apparatus of the present invention, the diameter of the first tube 8 and/or the second tube 10 is 2.5cm, the second tube 10 is adhered to the sidewall of the cultivation main body 1, and the diameter of the elbow 13 is 3.5 cm.

In the preferred embodiment of the microalgae culturing device of the invention, the power of the LED lamp tube 11 is 25 watts, the color temperature is 6500K, the length of the LED lamp tube 11 is 75cm, and the diameter is 2.5 cm.

In the preferred embodiment of the microalgae cultivation device of the invention, the LED lamp tube 11 is vertically placed on the bottom wall of the rectangular box 2, wherein the distance between the LED lamp tube 11 and the side wall of the rectangular box 2 is 10 cm.

In a preferred embodiment of the microalgae cultivation apparatus of the present invention, the wall of the second tube 10 has 100 micropores with a diameter of 5 mm.

The microalgae culture apparatus of the present invention can be manufactured and used in the field of microalgae.

Although the embodiments of the present invention have been described above with reference to the accompanying drawings, the present invention is not limited to the above-described embodiments and application fields, and the above-described embodiments are illustrative, instructive, and not restrictive. Those skilled in the art, having the benefit of this disclosure, may effect numerous modifications thereto without departing from the scope of the invention as defined by the appended claims.

Claims

1. The utility model provides a little algae culture apparatus, its includes the cultivation main part (1) of cuboid and arranges cultivation main part (1) inside cuboid box (2), cuboid box (2) with cultivation main part (1) sharing roof (3), its characterized in that: the top wall (3) of the culture main body (1) is provided with a first round hole (4) and a second round hole (5), the bottom wall (6) of the culture main body (1) is provided with an annular nano tube (7) around the cuboid box (2), a first tube (8) inserted into the first round hole (4) is connected with an upper opening of a reducing tee (9), a left opening and a right opening of the reducing tee (9) are communicated with the nano tube (7), a second tube (10) inserted into the second round hole (5) is fixed on the side wall of the culture main body (1), the nano tube (7) and the second tube (10) are provided with a plurality of micropores, wherein the density of the micropores of the nano tube (7) is 700 and 1200 micropores/m, the inner wall of the cuboid box (2) is provided with at least one LED lamp tube (11), the bottom wall (6) of the culture main body (1) is provided with a third round hole (12), one end of the elbow (13) is connected with the third round hole (12), the LED lamp tube (11) is vertically placed on the bottom wall of the cuboid box (2), and the distance between the LED lamp tube (11) and the side wall of the cuboid box (2) is 10 cm.

2. The microalgae cultivation apparatus as claimed in claim 1, characterized in that: the top wall (3) is provided with a handle (14), and the bottom wall (6) is provided with a column base (15) for supporting.

3. The microalgae cultivation apparatus as claimed in claim 1, characterized in that: cultivate main part (1) and cuboid box (2) and make by ya keli material, first pipe (8) and second pipe (10) are made by the PVC material.

4. The microalgae cultivation apparatus as claimed in claim 1, characterized in that: the length of the culture main body (1) is 100cm, the width is 40cm, the height is 80cm and the thickness is 1 cm.

5. The microalgae cultivation apparatus as claimed in claim 4, characterized in that: the first round hole (4) is 5cm away from the rear wall of the culture main body (1) and 50cm away from the side wall of the culture main body (1), the diameter of the first round hole (4) is 3cm, the diameter of the second round hole (5) is 3cm, and the diameter of the third round hole (12) is 3.5 cm.

6. The microalgae cultivation apparatus as claimed in claim 1, characterized in that: the cuboid box (2) is 30cm long, 20cm wide, 80cm high and 0.8cm thick.

7. The microalgae cultivation apparatus as claimed in claim 1, characterized in that: the distance between the left side wall of the cuboid box (2) and the left side wall of the culture main body (1) is 35cm, and the distance between the front wall of the cuboid box (2) and the front wall of the culture main body (1) is 10 cm.

8. The microalgae cultivation apparatus as claimed in claim 1, characterized in that: the diameter of the first pipe (8) and/or the second pipe (10) is 2.5cm, the second pipe (10) is adhered to the side wall of the culture main body (1), and the diameter of the elbow (13) is 3.5 cm.

9. The microalgae cultivation apparatus as claimed in claim 1, characterized in that: the power of the LED lamp tube (11) is 25 watts, the color temperature is 6500K, the length of the LED lamp tube (11) is 75cm, and the diameter is 2.5 cm.