CN108003219A - A kind of can improve cracks rice protein extracting ratio and carries out the method for glycosylation modification to it - Google Patents
A kind of can improve cracks rice protein extracting ratio and carries out the method for glycosylation modification to it Download PDFInfo
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- CN108003219A CN108003219A CN201711313093.3A CN201711313093A CN108003219A CN 108003219 A CN108003219 A CN 108003219A CN 201711313093 A CN201711313093 A CN 201711313093A CN 108003219 A CN108003219 A CN 108003219A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
- C07K1/1077—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups by covalent attachment of residues other than amino acids or peptide residues, e.g. sugars, polyols, fatty acids
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Abstract
The invention discloses a kind of can improve to crack rice protein extracting ratio and carries out the method for glycosylation modification to it, powder of cracking rice is handled with reference to heat treatment using suitable super-pressure first, the recovery rate of albumin in rice, globulin, alcohol soluble protein and glutelin can be significantly improved, reduce glutelin ordered structure content to make it easier for dissolving, be conducive to the extraction of follow-up rice gluten；Then it will crack rice and be extracted in alkaline solution, albumen dissolubility in alkaline solution of cracking rice is preferable, has the advantages that the time is short, temperature is low, energy consumption is few using ultrasonic wave assisted extraction albumen of cracking rice, while obtained albumen of cracking rice also possesses good functional characteristic；Finally rice gluten is modified using lactose, under the action of ultrasonic wave, albumen solubility after glycosylation modification significantly improves, emulsifying activity improves and emulsion stability strengthens, expose hydrophobic grouping, the surface hydrophobic enhancing of former protein molecular, can be used as food additives etc., is more conducive to absorption of human body.
Description
Technical field
The present invention relates to rice deep process technology field, more particularly to one kind can improve the protein extracting ratio and to it of cracking rice
The method for carrying out glycosylation modification.
Background technology
For a long time, China's paddy processing is in the state for only meeting grain ration, and 4 caused by annual paddy processing
0000000 tons of rice husks, more than 3,000 ten thousand tons crack rice, the valuable byproduct such as more than 1 400 ten thousand tons of rice brans is not yet opened well
Hair utilizes.The substantial amounts of paddy conversion in China at present and deep processing are the task of top priority.Implement rice deep processing efficiently to rise in value complete utilization
Industrialization technology, can make China make the country prosperous from eating rice production big country to eating rice production transformation, and poorly efficient agricultural changes to high-efficiency agriculture.It is broken
Rice is accessory substance caused by husk rice, and nutritive value and whole rice are essentially identical, but utilization rate is only the 30% of whole rice.Rice gluten is
One kind resistance immunogenic peptide, is the good albumen source of infant and allergic human population.Rice gluten is complete because of its Coordinate specific of essential amino acid
It is whole, the advantages that utilization rate is high and easily digested and enjoy favor.Therefore from middle these nutritive value poles of extraction of cracking rice
High rice gluten is with good economic efficiency and application value.
The prior art, which has often to crack rice, is further ground into the extraction that rice gluten is carried out after rice flour, complicated in extraction process
And recovery rate is low, it is modified accordingly to improve the recovery rate of rice gluten, it is necessary to be handled before the extraction cracked rice, so that
It can improve subsequently to the efficiency of rice protein.However, albumen of cracking rice is mainly a kind of alkali solubility albumen, surface hydrophobic it is high and
There are more disulfide bond, dissolubility is low under conditions of neutral pH, so as to influence its functional characteristic and in production should
With, and the glutelin of water-insoluble accounts for 80% or so in albumen of cracking rice, therefore research is modified to albumen of cracking rice to improve
Dissolubility is of great significance.Lactose is one of important nutrient of growth and development, has facilitation simultaneously can intellectual development
Enough promote the growth of enteron aisle lactic acid bacteria, be conducive to intestinal health.Lactose can promote the absorption of calcium in the metabolic process of calcium, and low
After the rice gluten of quick property combines, the functional characteristic of rice gluten is enhanced, makes that it is more easy to be absorbed by the body and nutritive value maximizes,
Therefore need the rice glutens extracted to carry out glycosylation modification, to meet health of people demand, so as to improve industrial value more.
The content of the invention
The object of the invention be exactly in order to make up prior art the defects of, there is provided one kind can improve crack rice protein extracting ratio and
The method of glycosylation modification is carried out to it.
The present invention is achieved by the following technical solutions：
A kind of can improve cracks rice protein extracting ratio and carries out the method for glycosylation modification, including step in detail below to it：
（1）It will crack rice and be crushed after cleaning with pulverizer, and cross 100 mesh sieves, rice meal is made, the rice meal made be added suitable
The deionized water of amount is uniformly mixed, and vacuum sealing in Polythene Bag is added after being stirred, and the sample after encapsulation is put into high pressure and sets
In standby pressure chamber, hyperbaric heating is handled 15-20 minutes, is then decompressed to normal barometric pressure with the release speed of 100MPa/s；
（2）The 95% ethanol fast dewatering by the rice meal taken out from high-tension apparatus with 8-10 times of volume, filters, then to big
The absolute ethyl alcohol infiltration of meter Ti Ji equivalent, filters after mixing, and gained rice meal crushes at 40-45 DEG C after forced air drying, crosses 100
Mesh sieve, with valve bag it is packaged be placed on it is spare in drier；
（3）By step（2）The rice meal handled well adds suitable deionized water and adjusts liquid ratio, the hydrogen for being 10wt% with concentration
It is 9.5-10 that sodium hydroxide solution, which adjusts pH, 60-65 DEG C of temperature control, ultrasonic wave extraction 30-40 minutes, then at 3500-400 revs/min
Speed under centrifuge 20-25 minutes, take supernatant, it is 4.2-4.5 to add the salt acid for adjusting pH that appropriate concentration is 10wt%, continue
Centrifuged 20-25 minutes under 3500-400 revs/min of speed, take precipitation, be washed with deionized water 2-3 times, be then freeze-dried, obtain
To albumen of cracking rice；
（4）The albumen that will crack rice mixes according to a certain percentage with lactose, is dissolved in the phosphate buffer of appropriate 0.1mol/L, control
Rice gluten concentration is 12-16mg/mL, magnetic agitation 60-90 minutes at room temperature, adds the sodium azide of rice gluten quality 0.5%,
Mixture is preheating to 90-100 DEG C and is placed on processing a period of time in Vltrasonic device, is immediately placed in after reaction cold in ice bath
But come that reaction was completed, then when the 20-24 that dialyses is small, be finally freeze-dried obtained liquid.
A kind of can improve cracks rice protein extracting ratio and carries out the method for glycosylation modification, step to it（1）In
The suitable deionized water mixing of the addition, specific amount of water is 50%.
A kind of can improve cracks rice protein extracting ratio and carries out the method for glycosylation modification, step to it（1）In
The high heat treatment of the high pressure, actual conditions are：600MPa is boosted to the rate of rise of 50MPa/s, temperature maintains 45 DEG C.
A kind of can improve cracks rice protein extracting ratio and carries out the method for glycosylation modification, step to it（3）In
The adjusting liquid ratio, specific liquid ratio are 1:12.
A kind of can improve cracks rice protein extracting ratio and carries out the method for glycosylation modification, step to it（3）In
The ultrasonic wave, the power of specific ultrasonic wave is 120W.
A kind of can improve cracks rice protein extracting ratio and carries out the method for glycosylation modification, step to it（4）In
The albumen of cracking rice mixes according to a certain percentage with lactose to be referred to according to 55:45 ratio mixing.
A kind of can improve cracks rice protein extracting ratio and carries out the method for glycosylation modification, step to it（4）In
A period of time is handled in the Vltrasonic device, actual conditions refers to ultrasonic power 300W, ultrasonic time 30 minutes.
It is an advantage of the invention that：The present invention is first handled powder of cracking rice with reference to heat treatment using suitable super-pressure,
The solubility and gel expansion rate of powder of cracking rice can be effectively improved, and can significantly be carried under super-pressure combination thermal processes act
Albumin in high rice, globulin, the recovery rate of alcohol soluble protein and glutelin, reducing glutelin ordered structure content is more easy to it
In dissolving, be conducive to the extraction of follow-up rice gluten；Then it will crack rice and be extracted in alkaline solution, albumen of cracking rice is molten in alkalescence
Dissolubility is preferable in liquid, has the advantages that the time is short, temperature is low, energy consumption is few using ultrasonic wave assisted extraction albumen of cracking rice, at the same time
Obtained albumen of cracking rice also possesses good functional characteristic；Finally rice gluten is modified using lactose, in the work of ultrasonic wave
Under, the albumen solubility after glycosylation modification significantly improves, and emulsifying activity improves and emulsion stability strengthens, and makes hydrophobic grouping
Exposure, the surface hydrophobic enhancing of former protein molecular, can be used as food additives etc., is more conducive to absorption of human body.
Embodiment
A kind of can improve cracks rice protein extracting ratio and carries out the method for glycosylation modification to it, including walks in detail below
Suddenly：
（1）It will crack rice and be crushed after cleaning with pulverizer, and cross 100 mesh sieves, rice meal is made, the rice meal made will be added
50% deionized water is uniformly mixed, and vacuum sealing in Polythene Bag is added after being stirred, and the sample after encapsulation is put into high pressure and sets
In standby pressure chamber, 600MPa is boosted to the rate of rise of 50MPa/s, temperature maintains 45 DEG C, and hyperbaric heating is handled 15 minutes,
Then normal barometric pressure is decompressed to the release speed of 100MPa/s；
（2）The 95% ethanol fast dewatering by the rice meal taken out from high-tension apparatus with 8 times of volumes, filters, then to rice body
The absolute ethyl alcohol infiltration of product equivalent, filters after mixing, and gained rice meal crushes at 40 DEG C after forced air drying, crosses 100 mesh sieves, uses
Valve bag is packaged be placed on it is spare in drier；
（3）By step（2）It is 1 that the rice meal handled well, which adds suitable deionized water and adjusts liquid ratio,:12, be with concentration
It is 9.5 that the sodium hydroxide solution of 10wt%, which adjusts pH, and 60 DEG C of temperature control, is extracted 30 minutes, Ran Hou with the power ultrasonic of 120W
Centrifuged 20 minutes under 3500 revs/min of speed, take supernatant, it is 4.2 to add the salt acid for adjusting pH that appropriate concentration is 10wt%, after
Continue and centrifuged 20 minutes under 3500 revs/min of speed, take precipitation, be washed with deionized water 2 times, be then freeze-dried, cracked rice
Albumen；
（4）Albumen and lactose will be cracked rice by 55:45 ratio mixing, is dissolved in the phosphate buffer of appropriate 0.1mol/L, controls
Rice milling protein concentration is 12mg/mL, at room temperature magnetic agitation 60 minutes, adds the sodium azide of rice gluten quality 0.5%, will be mixed
Compound is preheating to 90 DEG C and is placed in Vltrasonic device, control ultrasonic power 300W, ultrasonic time 30 minutes, after reaction immediately
Be put into ice bath cool down come reaction was completed, then dialyse 20 it is small when, finally obtained liquid is freeze-dried.
Claims (7)
1. a kind of can improve cracks rice protein extracting ratio and carries out the method for glycosylation modification to it, it is characterised in that including with
Lower specific steps：
（1）It will crack rice and be crushed after cleaning with pulverizer, and cross 100 mesh sieves, rice meal is made, the rice meal made be added suitable
The deionized water of amount is uniformly mixed, and vacuum sealing in Polythene Bag is added after being stirred, and the sample after encapsulation is put into high pressure and sets
In standby pressure chamber, hyperbaric heating is handled 15-20 minutes, is then decompressed to normal barometric pressure with the release speed of 100MPa/s；
（2）The 95% ethanol fast dewatering by the rice meal taken out from high-tension apparatus with 8-10 times of volume, filters, then to big
The absolute ethyl alcohol infiltration of meter Ti Ji equivalent, filters after mixing, and gained rice meal crushes at 40-45 DEG C after forced air drying, crosses 100
Mesh sieve, with valve bag it is packaged be placed on it is spare in drier；
（3）By step（2）The rice meal handled well adds suitable deionized water and adjusts liquid ratio, the hydrogen for being 10wt% with concentration
It is 9.5-10 that sodium hydroxide solution, which adjusts pH, 60-65 DEG C of temperature control, ultrasonic wave extraction 30-40 minutes, then at 3500-400 revs/min
Speed under centrifuge 20-25 minutes, take supernatant, it is 4.2-4.5 to add the salt acid for adjusting pH that appropriate concentration is 10wt%, continue
Centrifuged 20-25 minutes under 3500-400 revs/min of speed, take precipitation, be washed with deionized water 2-3 times, be then freeze-dried, obtain
To albumen of cracking rice；
（4）The albumen that will crack rice mixes according to a certain percentage with lactose, is dissolved in the phosphate buffer of appropriate 0.1mol/L, control
Rice gluten concentration is 12-16mg/mL, magnetic agitation 60-90 minutes at room temperature, adds the sodium azide of rice gluten quality 0.5%,
Mixture is preheating to 90-100 DEG C and is placed on processing a period of time in Vltrasonic device, is immediately placed in after reaction cold in ice bath
But come that reaction was completed, then when the 20-24 that dialyses is small, be finally freeze-dried obtained liquid.
2. a kind of can improve according to claim 1 cracks rice protein extracting ratio and carries out the side of glycosylation modification to it
Method, it is characterised in that step（1）Described in the mixing of addition suitable deionized water, specific amount of water is 50%.
3. a kind of can improve according to claim 1 cracks rice protein extracting ratio and carries out the side of glycosylation modification to it
Method, it is characterised in that step（1）Described in the high heat treatment of high pressure, actual conditions is：Boosted with the rate of rise of 50MPa/s
To 600MPa, temperature maintains 45 DEG C.
4. a kind of can improve according to claim 1 cracks rice protein extracting ratio and carries out the side of glycosylation modification to it
Method, it is characterised in that step（3）Described in adjusting liquid ratio, specific liquid ratio be 1:12.
5. a kind of can improve according to claim 1 cracks rice protein extracting ratio and carries out the side of glycosylation modification to it
Method, it is characterised in that step（3）Described in ultrasonic wave, the power of specific ultrasonic wave is 120W.
6. a kind of can improve according to claim 1 cracks rice protein extracting ratio and carries out the side of glycosylation modification to it
Method, it is characterised in that step（4）Described in albumen of cracking rice mix and refer to according to 55 according to a certain percentage with lactose:45 ratio
Example mixing.
7. a kind of can improve according to claim 1 cracks rice protein extracting ratio and carries out the side of glycosylation modification to it
Method, it is characterised in that step（4）Described in Vltrasonic device in handle a period of time, actual conditions refers to ultrasonic power 300W,
Ultrasonic time 30 minutes.
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| CN201711313093.3A CN108003219A (en) | 2017-12-12 | 2017-12-12 | A kind of can improve cracks rice protein extracting ratio and carries out the method for glycosylation modification to it |
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| CN201711313093.3A CN108003219A (en) | 2017-12-12 | 2017-12-12 | A kind of can improve cracks rice protein extracting ratio and carries out the method for glycosylation modification to it |
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Cited By (5)
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|---|---|---|---|---|
| CN110679724A (en) * | 2019-09-16 | 2020-01-14 | 天津科技大学 | Preparation method of easy-to-absorb glycated glutenin |
| CN110720544A (en) * | 2019-09-16 | 2020-01-24 | 天津科技大学 | Preparation method of glycosylated glutenin |
| CN111410917A (en) * | 2020-05-06 | 2020-07-14 | 江西师范大学 | Preparation method of fish skin gelatin-based surfactant |
| EP3564254A4 (en) * | 2016-12-27 | 2020-10-21 | Spiber Inc. | Method for recovering protein |
| CN113831961A (en) * | 2021-09-22 | 2021-12-24 | 中国农业大学 | Walnut oil extraction method |
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Cited By (6)
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| EP3564254A4 (en) * | 2016-12-27 | 2020-10-21 | Spiber Inc. | Method for recovering protein |
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| CN113831961A (en) * | 2021-09-22 | 2021-12-24 | 中国农业大学 | Walnut oil extraction method |
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