CN107996392A - A kind of selection of the special Restoring Line of Japonica Rice of beer - Google Patents

A kind of selection of the special Restoring Line of Japonica Rice of beer Download PDF

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CN107996392A
CN107996392A CN201711373484.4A CN201711373484A CN107996392A CN 107996392 A CN107996392 A CN 107996392A CN 201711373484 A CN201711373484 A CN 201711373484A CN 107996392 A CN107996392 A CN 107996392A
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rice
plant
strain
single plant
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章清杞
陈幼玉
李美德
杨帆
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Fujian Agriculture and Forestry University
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Fujian Agriculture and Forestry University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
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  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention discloses a kind of selection of the special Restoring Line of Japonica Rice of beer, belongs to Special Rices breeding technical field.The present invention selects head rice rate high, short round shaped grain type, high yield japonica rice strain, the low japonica rice strain of gelatinization point, restoring force is strong, coordinate force is good, rice starch arrangement is loose, the high long-grained nonglutinous rice strain of amylose content, high yield, it is disease-resistant, cold-resistant japonica rice strain makees parent, screened by the hybridization combo and objective trait of science, select high yield, it is cold-resistant, it is disease-resistant, head rice rate is high, short round shaped grain type, gelatinization point is low, starch arrangement is loose, amylose content is high, the special Restoring Line of Japonica Rice of the good beer of coordinate force, the rice of the hybrid paddy rice of the restorer or its combo can be used for beer brewing, peasant planting can increase income more than 500 yuan per acre.The method of the present invention is scientific and efficient, easily operated, and the selection and breeding to special rice kind have definite meaning.

Description

A kind of selection of the special Restoring Line of Japonica Rice of beer
Technical field
Present invention relates particularly to a kind of selection of the special Restoring Line of Japonica Rice of beer, belongs to Special Rices breeding technique neck Domain.
Background technology
Beer is universally acknowledged nutraceutical, and flourishing long time international beverage, is known as the good reputation of " liquid bread ". In the latest 20 years, the beer industry in China is fast-developing, and beer production accounts for nearly 2/3rds of national drinks total amount, and in year by year Ascendant trend, yield rank first in the world, and become global maximum beer production and marketing state.For a long time, fructus hordei germinatus is superior with its Characteristic and as the primary raw material of beer brewing, numerous beer producers use import barley, and the factors of instability are very much.By In the rise of raw material heavy dependence import, particularly malt price so that many beer producers production costs ramp, The market competitiveness weakens, and economic benefit declines.Therefore the fine quality rice raw material that exploitation can substitute fructus hordei germinatus is an important technical Problem.Using rice as major ingredient, and auxiliary material is made with fructus hordei germinatus, while add a certain proportion of burnt malt and make modifying agent, addition one Quantitative enzyme preparation, rice ratio can bring up to more than 50%.Different rice varieties to brewing performance there are notable difference, And this species diversity is often difficult to make up by process means.At home to the selection and breeding and research of quality beer rice special kind Almost blank, thus be necessary to cultivate a kind of new rice variety with high-quality brewing quality and characteristic to mix instead of multi items The rice of conjunction.
Research shows that it is short round shaped grain to be generally the appearance requirement grain of rice in the breeding objective of quality beer rice special, and starch arranges It is loose, amylose content is high, gelatinization point is low.Rice, which is applied to beer brewing tool, to have great advantage, and rice pest insects enrich, product Various, the ample supply and prompt delivery of kind, and price is cheaper than fructus hordei germinatus.Under the shortage of high-quality beer wheat, malt rise in price sternness situation, beer Raw material oneself become a critically important new problem being faced of our times brewing industry.Not good raw material, then good brewing Person also can not make quality beer.It is contemplated that selected in the future with high adjunct rice to make the rice special kind of quality beer Educate and industry development is by as the hot spot of vast beer producers and related scientific research personnel concern.
Rice yield is high, price is less than international beer wheat, in the case of China's beer raw material shortage and ensures beer quality On the premise of unaffected, increase auxiliary material rice ratio, reduce fructus hordei germinatus dosage, give full play to rice pest insects advantage, undoubtedly will Huge impetus is played to the sound and sustained development of China's Beer Industry.The present invention selects that head rice rate is high, short round shaped grain Type, high yield japonica rice strain, the low japonica rice strain of gelatinization point, restoring force is strong, coordinate force is good, rice starch arranges loose, straight chain The high long-grained nonglutinous rice strain of content of starch, high yield, disease-resistant, cold-resistant japonica rice strain make parent, pass through the hybridization combo and target of science Character screening, selects high yield, cold-resistant, disease-resistant, head rice rate is high, short round shaped grain type, gelatinization point are low, starch arrangement is loose, straight The rice of the hybrid paddy rice of the special Restoring Line of Japonica Rice of beer that chain content of starch is high, coordinate force is good, the restorer or its combo can use In beer brewing, peasant planting can increase income more than 500 yuan per acre.The method of the present invention is scientific and efficient, easily operated, is The industrialization development of beer rice lays the foundation.
The content of the invention
It is an object of the invention to the deficiency for existing breeding technique, there is provided a kind of to be recovered with the japonica rice of beer brewing The selection of system.For the present invention with head rice rate is high, short round shaped grain type, high yield japonica rice strain, the low japonica rice strain of gelatinization point is extensive The multiple long-grained nonglutinous rice strain that power is strong, coordinate force is good, rice starch arrangement is loose, amylose content is high, high yield, disease-resistant, cold-resistant round-grained rice Rice strain makees parent, is screened by the hybridization combo and objective trait of science, selects high yield, cold-resistant, disease-resistant, head rice rate High, short round shaped grain type, gelatinization point are low, starch arranges the special japonica rice of beer loose, that amylose content is high, coordinate force is good and recovers System.
To achieve the above object, the present invention adopts the following technical scheme that and selection and breeding step：
1）Using head rice rate is high, short round shaped grain type, high yield japonica rice strain as female parent, be denoted as kind P1；With the low japonica rice of gelatinization point Strain P2, restoring force is strong, coordinate force is high, amylose content is high, rice starch arranges loose long-grained nonglutinous rice strain P3, high yield, adaptation Property wide, cold-resistant blast resisting japonica rice strain P4 be male parent；Make hybridization P1/P2, P1/P3 and P1/P4, obtain first generation of hybrid F1-12、F1-13 And F1-14；
2）Plant first generation of hybrid F1-12、F1-13And F1-14, florescence is with F1-12Make it is maternal respectively with F1-13And F1-14Hybridize again, obtain Must be reestablished diplomatic relations a generation, with F1-123And F1-124Represent；
3）Plant F1-123And F1-124, florescence takes young fringe respectively, 73wt% alcohol disinfectings, is handled 4 days at 8.2 DEG C；Ultra-clean work Young fringe, 0.1wt%HgCl are peeled off on platform2Solution disinfection 10 minutes, sterile water wash 5 times, is inoculated in after drying up flower pesticide surface moisture Evoked callus on SK3 culture mediums, inoculum concentration are 3000-3200 flower pesticide, and callus subculture is placed on N6 culture mediums 1 time Middle carry out induction, is placed in root induction in the root media of the colchicine containing 3.3mg/L by the differentiation seedling of acquisition and adds Cultivate again, grow up to seedling, be finally transferred to take root on the MS culture mediums of no hormone, grow up to plantlet, then be transplanted in greenhouse and plant Plant, removes single times and polyploid, retains liploid plant, and single plant sowing obtains DH colonies after maturation, respectively with DH1-1 and DH1-2 Represent；
4）Spring sowing plantation DH1-1 and DH1-2, each colony 800-900 plants, DH1-2 carries out rice blast in seedling stage and resists cold Property identification, pull out not anti-strain, retain cold-resistant disease-resistant plant；Ear in summer ripe, the short round shaped grain type of DH1-1 colonies selection, Setting percentage obtains DH2-1 up to the excellent single plant harvest seed of more than 88% and economical character, and DH1-2 colonies select short round shaped grain type, solid Rate obtains DH2-2 up to the excellent single plant harvest seed of more than 88% and economical character；
5）Screening is processed to DH2-1, DH2-2 single-strain seed of harvest using miniature rice mill in laboratory, is therefrom selected White belly is big, the single plant of grain length ﹤ 5.5mm, Coarse Rice Rate ﹥ 80% and head rice rate ﹥ 65%；
6）To 5）The DH2-1 single plants rice of upper choosing carries out amylose content and gelatinization point measure, selects amylose content ﹥ The single plant of 65 DEG C of 25% and gelatinization point ﹤, carries out rice starch arrangement density using scanning electron microscope and identifies, select starch arrangement to dredge The single plant of pine；To 5）The DH2-2 single plants rice of upper choosing carries out gelatinization point measure, selects the single plant of 65 DEG C of gelatinization point ﹤；
7）Sowing 6）Screening gained DH2-1, DH2-2 single plant, florescence select plant height 105- respectively in DH2-1, DH2-2 single plant 3 plants of the single plant that 115cm, sword-like leave are upright, fringe head is larger and pollen loose powder is good, makees hybridization DH2-1/DH2-2, obtain first familiar generation strain with F1-1234Represent；
8）Plant F1-1234, florescence takes young fringe, 73wt% alcohol disinfectings, is handled 4 days at 8.2 DEG C, children is peeled off on superclean bench Fringe, 0.1wt%HgCl2Solution disinfection 10 minutes, sterile water wash 5 times, SK3 culture mediums are inoculated in after drying up flower pesticide surface moisture Upper evoked callus, inoculum concentration are 3300-3500 flower pesticide, and callus subculture, which is placed in N6 culture mediums for 1 time, to be divided Change induction, the differentiation seedling of acquisition is placed in root induction in the root media of the colchicine containing 3.3mg/L and doubles to cultivate, it is long Into seedling, be transferred on the MS culture mediums of no hormone and take root, grow up to plantlet, then be transplanted in greenhouse and plant, remove single times and Polyploid, retains liploid plant, and single plant sowing obtains DH colonies after maturation, is represented with DH1-1234；
9）Spring sowing plantation DH1-1234, colony 850-950 plants, seedling stage carries out rice blast and Evaluation of Cold Tolerance, pulls out not Anti- strain, retains disease-resistant cold-resistant plant；Ear in summer ripe, select short round shaped grain type, setting percentage up to more than 88% and economical character Excellent round-grained rice type strain, single plant harvest seed obtain DH2-1234；
10）Screening is processed to the DH2-1234 of harvest using miniature rice mill in laboratory, therefrom select white belly greatly, grain length The single plant of ﹤ 5.5mm, Coarse Rice Rate ﹥ 80% and head rice rate ﹥ 65%；The single plant rice of upper choosing carries out amylose content and gelatinization temperature Degree measure, selects the single plant of 65 DEG C of amylose content ﹥ 25% and gelatinization point ﹤, and rice starch arrangement is carried out using scanning electron microscope Density is identified, selects starch to arrange loose single plant；
11）Plantation 10）The single plant of upper choosing, while plant test cross sterile line, seedling stage carry out the single plant of upper choosing rice blast and resistance to Cold identification, pulls out not anti-strain, retains disease-resistant cold-resistant plant；Florescence carries out upper menu strain test cross, maturity period selection Setting percentage obtains DH3- up to the round-grained rice type strain that more than 85%, yield traits is good, big panicle many grains per panicle and blade annesl are good, single plant harvest seed 1234；
12）Strain plantation 11）The DH3-1234 single plants and test cross F1 of upper choosing, maturity period investigation test cross F1 setting percentages and yield traits Performance, the corresponding DH3-1234 strains of test cross F1 for selecting setting percentage more than 85% and yield traits to do very well, therefrom selects agronomy Character and yield traits show excellent single plant sowing, that is, are bred as the special Restoring Line of Japonica Rice of beer.
A kind of selection of the special Restoring Line of Japonica Rice of beer of the present invention, the head rice rate is high, short round shaped grain type, High yield japonica rice strain P1 is military round-grained rice 075, and the low japonica rice strain P2 of gelatinization point is salt round-grained rice 2, and restoring force is strong, coordinate force is high, straight Chain content of starch is high, the loose long-grained nonglutinous rice strain P3 of rice starch arrangement is Lu extensive 6684 or Lu extensive 37, and high yield, wide adaptability, resist cold Blast resisting japonica rice strain P4 is beautiful round-grained rice 314.
Beneficial effects of the present invention：
Using the method for the present invention can select high yield, cold-resistant, disease-resistant, head rice rate is high, short round shaped grain type, gelatinization point are low, starch Arrange the Restoring Line of Japonica Rice for the suitable brewing that loose, amylose content is high, coordinate force is good.The special rice yield of beer brewing High, price is less than beer wheat, in the case of China's beer raw material shortage, increases auxiliary material rice ratio, reduces fructus hordei germinatus dosage, China's rice pest insects advantage is given full play to, huge promotion will be undoubtedly played to the sound and sustained development of China's Beer Industry and is made With.The peasant planting kind can increase income more than 500 yuan per acre.The method of the present invention is scientific and efficient, easily operated, is beer The industrialization development of wine rice lays the foundation.
Embodiment：
Embodiment 1：
1）Select high head rice rate, short round shaped grain type, high yield japonica rice strain P1- forces round-grained rice 075, the low japonica rice strain P2- salt of gelatinization point Round-grained rice 2, restoring force is strong, coordinate force is high, amylose content is high, rice starch arranges loose long-grained nonglutinous rice strain P3- Lus extensive 6684, high Production, wide adaptability, the beautiful round-grained rice 314 of cold-resistant blast resisting japonica rice strain P4-；Make hybridization P1/P2, P1/P3 and P1/P4, obtain hybrid one For F1-12、F1-13And F1-14；
2）Plant first generation of hybrid F1-12、F1-13And F1-14, florescence is with F1-12Make it is maternal respectively with F1-13And F1-14Hybridize again, obtain Must be reestablished diplomatic relations a generation, with F1-123And F1-124Represent；
3）Plant F1-123And F1-124, florescence takes young fringe respectively, 73wt% alcohol disinfectings, is handled 4 days at 8.2 DEG C；Ultra-clean work Young fringe, 0.1wt%HgCl are peeled off on platform2Solution disinfection 10 minutes, sterile water wash 5 times, is inoculated in after drying up flower pesticide surface moisture Evoked callus on SK3 culture mediums, inoculum concentration are 3000 flower pesticide, callus subculture be placed in N6 culture mediums for 1 time into Row induction, is placed in root induction in the root media of the colchicine containing 3.3mg/L by the differentiation seedling of acquisition and doubles to train Support, grow up to seedling, be finally transferred to take root on the MS culture mediums of no hormone, grow up to plantlet, then be transplanted in greenhouse and plant, go Except single times and polyploid, retain liploid plant, single plant sowing obtains DH colonies after maturation, is represented respectively with DH1-1 and DH1-2；
4）Spring sowing plantation DH1-1 and DH1-2, each 800 plants of colony, DH1-2 carry out rice blast and cold resistance mirror in seedling stage It is fixed, not anti-strain is pulled out, retains cold-resistant disease-resistant plant；Ear in summer ripe, DH1-1 colonies select short round shaped grain type, solid Rate obtains DH2-1 up to the excellent single plant harvest seed of more than 88% and economical character, and DH1-2 colonies select short round shaped grain type, setting percentage to reach More than the 88% single plant harvest seed excellent with economical character obtains DH2-2；
5）Screening is processed to DH2-1, DH2-2 single-strain seed of harvest using miniature rice mill in laboratory, is therefrom selected White belly is big, the single plant of grain length ﹤ 5.5mm, Coarse Rice Rate ﹥ 80% and head rice rate ﹥ 65%；
6）To 5）The DH2-1 single plants rice of upper choosing carries out amylose content and gelatinization point measure, selects amylose content ﹥ The single plant of 65 DEG C of 25% and gelatinization point ﹤, carries out rice starch arrangement density using scanning electron microscope and identifies, select starch arrangement to dredge The single plant of pine；To 5）The DH2-2 single plants rice of upper choosing carries out gelatinization point measure, selects the single plant of 65 DEG C of gelatinization point ﹤；
7）Sowing 6）Screening gained DH2-1, DH2-2 single plant, florescence select plant height 105- respectively in DH2-1, DH2-2 single plant 3 plants of the single plant that 115cm, sword-like leave are upright, fringe head is larger and pollen loose powder is good, makees hybridization DH2-1/DH2-2, obtain first familiar generation strain with F1-1234Represent；
8）Plant F1-1234, florescence takes young fringe, 73wt% alcohol disinfectings, is handled 4 days at 8.2 DEG C, children is peeled off on superclean bench Fringe, 0.1wt%HgCl2Solution disinfection 10 minutes, sterile water wash 5 times, SK3 culture mediums are inoculated in after drying up flower pesticide surface moisture Upper evoked callus, inoculum concentration are 3300 flower pesticide, and callus subculture, which is placed in N6 culture mediums for 1 time, to carry out differentiation and lure Lead, the differentiation seedling of acquisition is placed in root induction in the root media of the colchicine containing 3.3mg/L and doubles to cultivate, is grown up to small Seedling, is transferred on the MS culture mediums of no hormone and takes root, grow up to plantlet, then is transplanted in greenhouse and plants, and removes single times and more times Body, retains liploid plant, and single plant sowing obtains DH colonies after maturation, is represented with DH1-1234；
9）Spring sowing plantation DH1-1234,850 plants of colony, seedling stage carry out rice blast and Evaluation of Cold Tolerance, pull out not anti-strain, Retain disease-resistant cold-resistant plant；Ear in summer ripe, select short round shaped grain type, setting percentage excellent up to more than 88% and economical character Round-grained rice type strain, single plant harvest seed obtain DH2-1234；
10）Screening is processed to the DH2-1234 of harvest using miniature rice mill in laboratory, therefrom select white belly greatly, grain length The single plant of ﹤ 5.5mm, Coarse Rice Rate ﹥ 80% and head rice rate ﹥ 65%；The single plant rice of upper choosing carries out amylose content and gelatinization temperature Degree measure, selects the single plant of 65 DEG C of amylose content ﹥ 25% and gelatinization point ﹤, and rice starch arrangement is carried out using scanning electron microscope Density is identified, selects starch to arrange loose single plant；
11）Plantation 10）The single plant of upper choosing, while plant test cross sterile line, seedling stage carry out the single plant of upper choosing rice blast and resistance to Cold identification, pulls out not anti-strain, retains disease-resistant cold-resistant plant；Florescence carries out upper menu strain test cross, maturity period selection Setting percentage obtains DH3-1234 up to the strain that more than 85%, yield traits is good, big panicle many grains per panicle and blade annesl are good, single plant harvest seed；
12）Strain plantation 11）The DH3-1234 single plants and test cross F1 of upper choosing, maturity period investigation test cross F1 setting percentages and yield traits Performance, the corresponding DH3-1234 strains of test cross F1 for selecting setting percentage more than 85% and yield traits to do very well, therefrom selects agronomy Character and yield traits show excellent single plant sowing, that is, are bred as the special Restoring Line of Japonica Rice of beer-beer round-grained rice 1.
Beer round-grained rice 1 or the hybrid paddy rice rice of its combo can be used for beer brewing, and the beer flavor of brewing is good, No. 1 production of beer round-grained rice Amount is high, price is less than beer wheat, in the case of China's beer raw material shortage, increases auxiliary material rice ratio, reduces fructus hordei germinatus and uses Amount, gives full play to China's rice pest insects advantage, undoubtedly huge push away will be played to the sound and sustained development of China's Beer Industry Action is used.540 kilograms of per mu yield per acre of peasant planting beer round-grained rice 1, per acre than planting common 515 yuan of income of paddy increase.
Embodiment 2：
1）Select high head rice rate, short round shaped grain type, high yield japonica rice strain P1- forces round-grained rice 075, the low japonica rice strain P2- salt of gelatinization point Round-grained rice 2, restoring force is strong, coordinate force is high, amylose content is high, rice starch arranges loose long-grained nonglutinous rice strain P3- Lus extensive 37, high Production, wide adaptability, the beautiful round-grained rice 314 of cold-resistant blast resisting japonica rice strain P4-；Make hybridization P1/P2, P1/P3 and P1/P4, obtain hybrid one For F1-12、F1-13And F1-14；
2）Plant first generation of hybrid F1-12、F1-13And F1-14, florescence is with F1-12Make it is maternal respectively with F1-13And F1-14Hybridize again, obtain Must be reestablished diplomatic relations a generation, with F1-123And F1-124Represent；
3）Plant F1-123And F1-124, florescence takes young fringe respectively, 73wt% alcohol disinfectings, is handled 4 days at 8.2 DEG C；Ultra-clean work Young fringe, 0.1wt%HgCl are peeled off on platform2Solution disinfection 10 minutes, sterile water wash 5 times, is inoculated in after drying up flower pesticide surface moisture Evoked callus on SK3 culture mediums, inoculum concentration are 3200 flower pesticide, callus subculture be placed in N6 culture mediums for 1 time into Row induction, is placed in root induction in the root media of the colchicine containing 3.3mg/L by the differentiation seedling of acquisition and doubles to train Support, grow up to seedling, be finally transferred to take root on the MS culture mediums of no hormone, grow up to plantlet, then be transplanted in greenhouse and plant, go Except single times and polyploid, retain liploid plant, single plant sowing obtains DH colonies after maturation, is represented respectively with DH1-1 and DH1-2；
4）Spring sowing plantation DH1-1 and DH1-2, each 900 plants of colony, DH1-2 carry out rice blast and cold resistance mirror in seedling stage It is fixed, not anti-strain is pulled out, retains cold-resistant disease-resistant plant；Ear in summer ripe, DH1-1 colonies select short round shaped grain type, solid Rate obtains DH2-1 up to the excellent single plant harvest seed of more than 88% and economical character, and DH1-2 colonies select short round shaped grain type, setting percentage to reach More than the 88% single plant harvest seed excellent with economical character obtains DH2-2；
5）Screening is processed to DH2-1, DH2-2 single-strain seed of harvest using miniature rice mill in laboratory, is therefrom selected White belly is big, the single plant of grain length ﹤ 5.5mm, Coarse Rice Rate ﹥ 80% and head rice rate ﹥ 65%；
6）To 5）The DH2-1 single plants rice of upper choosing carries out amylose content and gelatinization point measure, selects amylose content ﹥ The single plant of 65 DEG C of 25% and gelatinization point ﹤, carries out rice starch arrangement density using scanning electron microscope and identifies, select starch arrangement to dredge The single plant of pine；To 5）The DH2-2 single plants rice of upper choosing carries out gelatinization point measure, selects the single plant of 65 DEG C of gelatinization point ﹤；
7）Sowing 6）Screening gained DH2-1, DH2-2 single plant, florescence select plant height 105- respectively in DH2-1, DH2-2 single plant 3 plants of the single plant that 115cm, sword-like leave are upright, fringe head is larger and pollen loose powder is good, makees hybridization DH2-1/DH2-2, obtain first familiar generation strain with F1-1234Represent；
8）Plant F1-1234, florescence takes young fringe, 73wt% alcohol disinfectings, is handled 4 days at 8.2 DEG C, children is peeled off on superclean bench Fringe, 0.1wt%HgCl2Solution disinfection 10 minutes, sterile water wash 5 times, SK3 culture mediums are inoculated in after drying up flower pesticide surface moisture Upper evoked callus, inoculum concentration are 3500 flower pesticide, and callus subculture, which is placed in N6 culture mediums for 1 time, to carry out differentiation and lure Lead, the differentiation seedling of acquisition is placed in root induction in the root media of the colchicine containing 3.3mg/L and doubles to cultivate, is grown up to small Seedling, is transferred on the MS culture mediums of no hormone and takes root, grow up to plantlet, then is transplanted in greenhouse and plants, and removes single times and more times Body, retains liploid plant, and single plant sowing obtains DH colonies after maturation, is represented with DH1-1234；
9）Spring sowing plantation DH1-1234,950 plants of colony, seedling stage carry out rice blast and Evaluation of Cold Tolerance, pull out not anti-strain, Retain disease-resistant cold-resistant plant；Ear in summer ripe, select short round shaped grain type, setting percentage excellent up to more than 88% and economical character Round-grained rice type strain, single plant harvest seed obtain DH2-1234；
10）Screening is processed to the DH2-1234 of harvest using miniature rice mill in laboratory, therefrom select white belly greatly, grain length The single plant of ﹤ 5.5mm, Coarse Rice Rate ﹥ 80% and head rice rate ﹥ 65%；The single plant rice of upper choosing carries out amylose content and gelatinization temperature Degree measure, selects the single plant of 65 DEG C of amylose content ﹥ 25% and gelatinization point ﹤, and rice starch arrangement is carried out using scanning electron microscope Density is identified, selects starch to arrange loose single plant；
11）Plantation 10）The single plant of upper choosing, while plant test cross sterile line, seedling stage carry out the single plant of upper choosing rice blast and resistance to Cold identification, pulls out not anti-strain, retains disease-resistant cold-resistant plant；Florescence carries out upper menu strain test cross, maturity period selection Setting percentage obtains DH3-1234 up to the strain that more than 85%, yield traits is good, big panicle many grains per panicle and blade annesl are good, single plant harvest seed；
12）Strain plantation 11）The DH3-1234 single plants and test cross F1 of upper choosing, maturity period investigation test cross F1 setting percentages and yield traits Performance, the corresponding DH3-1234 strains of test cross F1 for selecting setting percentage more than 85% and yield traits to do very well, therefrom selects agronomy Character and yield traits show excellent single plant sowing, that is, are bred as the special Restoring Line of Japonica Rice of beer-beer round-grained rice 2.
Beer round-grained rice 2 or the hybrid paddy rice rice of its combo can be used for beer brewing, and the beer flavor of brewing is good, No. 2 productions of beer round-grained rice Amount is high, price is less than beer wheat, in the case of China's beer raw material shortage, increases auxiliary material rice ratio, reduces fructus hordei germinatus and uses Amount, gives full play to China's rice pest insects advantage, undoubtedly huge push away will be played to the sound and sustained development of China's Beer Industry Action is used.550 kilograms of per mu yield per acre of peasant planting beer round-grained rice 2, per acre than planting common 520 yuan of income of paddy increase.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with Modification, should all belong to the covering scope of the present invention.

Claims (2)

  1. A kind of 1. selection of the special Restoring Line of Japonica Rice of beer, it is characterised in that：The selection comprises the following steps：
    1）Using head rice rate is high, short round shaped grain type, high yield japonica rice strain as female parent, kind P1 is denoted as, with the low japonica rice of gelatinization point Strain P2, restoring force is strong, coordinate force is high, amylose content is high, rice starch arranges loose long-grained nonglutinous rice strain P3, high yield, adaptation Property wide, cold-resistant blast resisting japonica rice strain P4 be male parent；Make hybridization P1/P2, P1/P3 and P1/P4, obtain first generation of hybrid F1-12、F1-13 And F1-14；
    2）Plant first generation of hybrid F1-12、F1-13And F1-14, florescence is with F1-12Make it is maternal respectively with F1-13And F1-14Hybridize again, obtain Must be reestablished diplomatic relations a generation, with F1-123And F1-124Represent；
    3）Plant F1-123And F1-124, florescence takes young fringe respectively, 73wt% alcohol disinfectings, is handled 4 days at 8.2 DEG C；Superclean bench It is upper to peel off young fringe, 0.1wt%HgCl2Solution disinfection 10 minutes, sterile water wash 5 times, is inoculated in after drying up flower pesticide surface moisture Evoked callus on SK3 culture mediums, inoculum concentration are 3000-3200 flower pesticide, and callus subculture is placed on N6 culture mediums 1 time Middle carry out induction, is placed in root induction in the root media of the colchicine containing 3.3mg/L by the differentiation seedling of acquisition and adds Cultivate again, grow up to seedling, be finally transferred to take root on the MS culture mediums of no hormone, grow up to plantlet, then be transplanted in greenhouse and plant Plant, removes single times and polyploid, retains liploid plant, and single plant sowing obtains DH colonies after maturation, respectively with DH1-1 and DH1-2 Represent；
    4）Spring sowing plantation DH1-1 and DH1-2, each colony 800-900 plants, DH1-2 carries out rice blast in seedling stage and resists cold Property identification, pull out not anti-strain, retain cold-resistant disease-resistant plant；Ear in summer ripe, the short round shaped grain type of DH1-1 colonies selection, Setting percentage obtains DH2-1 up to the excellent single plant harvest seed of more than 88% and economical character, and DH1-2 colonies select short round shaped grain type, solid Rate obtains DH2-2 up to the excellent single plant harvest seed of more than 88% and economical character；
    5）Screening is processed to DH2-1, DH2-2 single-strain seed of harvest using miniature rice mill in laboratory, is therefrom selected White belly is big, the single plant of grain length ﹤ 5.5mm, Coarse Rice Rate ﹥ 80% and head rice rate ﹥ 65%；
    6）To 5）The DH2-1 single plants rice of upper choosing carries out amylose content and gelatinization point measure, selects amylose content ﹥ The single plant of 65 DEG C of 25% and gelatinization point ﹤, carries out rice starch arrangement density using scanning electron microscope and identifies, select starch arrangement to dredge The single plant of pine；To 5）The DH2-2 single plants rice of upper choosing carries out gelatinization point measure, selects the single plant of 65 DEG C of gelatinization point ﹤；
    7）Sowing 6）Screening gained DH2-1, DH2-2 single plant, florescence select plant height 105- respectively in DH2-1, DH2-2 single plant 3 plants of the single plant that 115cm, sword-like leave are upright, fringe head is larger and pollen loose powder is good, makees hybridization DH2-1/DH2-2, obtain first familiar generation strain with F1-1234Represent；
    8）Plant F1-1234, florescence takes young fringe, 73wt% alcohol disinfectings, is handled 4 days at 8.2 DEG C, children is peeled off on superclean bench Fringe, 0.1wt%HgCl2Solution disinfection 10 minutes, sterile water wash 5 times, SK3 culture mediums are inoculated in after drying up flower pesticide surface moisture Upper evoked callus, inoculum concentration are 3300-3500 flower pesticide, and callus subculture, which is placed in N6 culture mediums for 1 time, to be divided Change induction, the differentiation seedling of acquisition is placed in root induction in the root media of the colchicine containing 3.3mg/L and doubles to cultivate, it is long Into seedling, be transferred on the MS culture mediums of no hormone and take root, grow up to plantlet, then be transplanted in greenhouse and plant, remove single times and Polyploid, retains liploid plant, and single plant sowing obtains DH colonies after maturation, is represented with DH1-1234；
    9）Spring sowing plantation DH1-1234, colony 850-950 plants, seedling stage carries out rice blast and Evaluation of Cold Tolerance, pulls out not Anti- strain, retains disease-resistant cold-resistant plant；Ear in summer ripe, select short round shaped grain type, setting percentage up to more than 88% and economical character Excellent round-grained rice type strain, single plant harvest seed obtain DH2-1234；
    10）Screening is processed to the DH2-1234 of harvest using miniature rice mill in laboratory, therefrom select white belly greatly, grain length The single plant of ﹤ 5.5mm, Coarse Rice Rate ﹥ 80% and head rice rate ﹥ 65%；The single plant rice of upper choosing carries out amylose content and gelatinization temperature Degree measure, selects the single plant of 65 DEG C of amylose content ﹥ 25% and gelatinization point ﹤, and rice starch arrangement is carried out using scanning electron microscope Density is identified, selects starch to arrange loose single plant；
    11）Plantation 10）The single plant of upper choosing, while plant test cross sterile line, seedling stage carry out the single plant of upper choosing rice blast and resistance to Cold identification, pulls out not anti-strain, retains disease-resistant cold-resistant plant；Florescence carries out upper menu strain test cross, maturity period selection Setting percentage obtains DH3- up to the round-grained rice type strain that more than 85%, yield traits is good, big panicle many grains per panicle and blade annesl are good, single plant harvest seed 1234；
    12）Strain plantation 11）The DH3-1234 single plants and test cross F1 of upper choosing, maturity period investigation test cross F1 setting percentages and yield traits Performance, the corresponding DH3-1234 strains of test cross F1 for selecting setting percentage more than 85% and yield traits to do very well, therefrom selects agronomy Character and yield traits show excellent single plant sowing, that is, are bred as the special Restoring Line of Japonica Rice of beer.
  2. A kind of 2. selection of the special Restoring Line of Japonica Rice of beer according to claim 1, it is characterised in that：The whole essence Rice rate is high, short round shaped grain type, high yield japonica rice strain P1 are military round-grained rice 075；The low japonica rice strain P2 of gelatinization point is salt round-grained rice 2, is recovered Power is strong, coordinate force is high, amylose content is high, to arrange loose long-grained nonglutinous rice strain P3 be Lu extensive 6684 or Lu extensive 37 to rice starch, height Production, wide adaptability, cold-resistant blast resisting japonica rice strain P4 are beautiful round-grained rice 314.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113261500A (en) * 2021-05-07 2021-08-17 湖南杂交水稻研究中心 Breeding method of special ultrahigh-yield hybrid rice for brewing Luzhou-flavor liquor

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113261500A (en) * 2021-05-07 2021-08-17 湖南杂交水稻研究中心 Breeding method of special ultrahigh-yield hybrid rice for brewing Luzhou-flavor liquor

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