CN107711490A - A kind of hybridizing method for improveing ilexrotunda winter resistance - Google Patents

A kind of hybridizing method for improveing ilexrotunda winter resistance Download PDF

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Publication number
CN107711490A
CN107711490A CN201710987694.6A CN201710987694A CN107711490A CN 107711490 A CN107711490 A CN 107711490A CN 201710987694 A CN201710987694 A CN 201710987694A CN 107711490 A CN107711490 A CN 107711490A
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ilexrotunda
seed
pollen
generation
hybridization
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CN201710987694.6A
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Chinese (zh)
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刘建军
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界首市铭硕园种植专业合作社
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Priority to CN201710987694.6A priority Critical patent/CN107711490A/en
Publication of CN107711490A publication Critical patent/CN107711490A/en

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination

Abstract

The invention discloses a kind of hybridizing method for improveing ilexrotunda winter resistance, including following aspect:(1)Pollen preserves, and 5 months April using excellent llex cornuta lindl plant as female parent, collects male pollen using sterilizing conical flask, low temperature, which is baked, is filled with nitrogen preservation in backward conical flask;(2)Hybridization, it will first preserve pollen in Second Year March to April and carry out activation process, respectively using liquor natrii hypochloritis and plant extract solution to being pollinated after Chinese ilex sprinkling during pollination;(3)Seed treatment, 1 generation seed is put into ammonification pond ammoniated treatment, then carries out intermittent warming;(4)Repeat to hybridize, according to step(2)Method, 1 generation seed of processing and llex cornuta lindl hybridization are cultivated into obtain 2 generation seeds, 2 generation seeds cultivate to obtain 3 generation seeds with llex cornuta lindl hybridization again, then 3 generation seeds are carried out breeding to cultivate in kind that ilexrotunda seed must be hybridized;(5)Cultivate, by hybridization ilexrotunda seed through step(3)Greenhouse cultivation is carried out after processing.

Description

A kind of hybridizing method for improveing ilexrotunda winter resistance

Technical field

The invention belongs to ilexrotunda planting technology field, and in particular to a kind of hybridizing method for improveing ilexrotunda winter resistance.

Background technology

Llex cornuta lindl is Ilex evergreen shrubs, and seeds are flourishing, leaf peculiar, and fruit after green maturation by turning For red, erythrocarpus is overworked in end of the branch, to Second Year ability in March shedding, can have higher ornamental value after pruned, frequently as Christmas tree uses;Llex cornuta lindl habit light, moistening and rich soil, it has higher winter resistance, can be in -8--10 DEG C of temperature The lower normal growth of degree, adaptability are stronger.Ilexrotunda is Aquifoliaceae evergreen shrubs, and alias iron holly bark, bear gall are wooden, when its fruit is ripe Take on a red color, wild tree species are born in the hillside evergreen broadleaf forest of 400~1100 meters of height above sea level more and border area, long in China at present On the south the basin of river, and there is plantation in the state such as Japan, Korea;It is usually used in landscape seeds, and leaf is thick and dense, you can plays screening The moon effect, can show the changeable landscape color of level, have higher ornamental value again.Ilexrotunda habit shade tolerant, happiness are born in Warm and moist weather and loose rich soil, it is suitable for being grown in the warm and humid area in subtropical zone, cold resistance is relatively poor, in Chinese north The relative cold district in side is not suitable for plantation breeding;And ilexrotunda seed shell is harder, natural germination percentage is relatively low, using sand storage Method can play emollescence, improve germination percentage, but storage time is longer, is not suitable for rapid scale plant development.

The content of the invention

The present invention is directed to the problem of existing:Warm and moist weather and loose rich soil are born in ilexrotunda habit shade tolerant, happiness, It is suitable for being grown in the warm and humid area in subtropical zone, cold resistance is relatively poor, with respect to cold district is not suitable for planting in north of China numerous Grow, and llex cornuta lindl has preferable winter resistance;And ilexrotunda seed shell is harder, natural germination percentage is relatively low, using sand Tibetan method can play emollescence, improve germination percentage, but storage time is longer, is not suitable for rapid scale plant development.For Solve the above problems, the invention provides a kind of hybridizing method for improveing ilexrotunda winter resistance.

The present invention is achieved by the following technical solutions:

A kind of hybridizing method for improveing ilexrotunda winter resistance, comprises the following steps:

(1)Pollen preserves:In April-May using excellent llex cornuta lindl plant as female parent, it can keep pollen that there is preferable heredity Shape, male pollen is collected using sterilizing conical flask, low temperature is filled with nitrogen into conical flask in 2-5 DEG C of condition after baking 16-19min Lower preservation, moisture and cytoactive in pollen are reduced, be filled with nitrogen and play insulation, the moistureproof and horizontal work of reduction cell metabolism With can for a long time be preserved to pollen, obtain preservation pollen;

(2)Hybridization:Pollen will be first preserved in Second Year March to April and carries out activation process, be improved the activity of pollen, be selected the iron winter Blue or green female plants pollination, in the morning 8 during pollination:00-9:00 respectively using liquor natrii hypochloritis and plant extract solution to Chinese ilex Flower sprinkling, can reduce microorganism is influenceed on pollination, and Chinese ilex pollen can be improved containing various plants active component in plant extraction liquid Activity, improve pollination success rate, dip in activation pollen using sterilizing hairbrush after natural air drying and pollinated, pollination 1 time daily, even It is continuous to carry out 3 days, 1 generation seed is obtained after culture;

(3)Seed treatment:1 generation seed is put into ammonification pond ammoniated treatment 75-80min, emollescence is played to kind of a shell, improves kind Sub- germination percentage, seed is then immersed into plant extraction liquid again and carries out intermittent warming 14-17h, improves tolerance energy of the seed to temperature Power, its resistance ability to external environment is improved, 1 generation seed must be handled;

(4)Repeat to hybridize:According to step(2)Method, 1 generation seed of processing and llex cornuta lindl hybridization are cultivated into obtain 2 generation seeds, 2 generations Seed cultivates to obtain 3 generation seeds with llex cornuta lindl hybridization again, and ilexrotunda can be made to obtain the stable excellent inhereditary feature of llex cornuta lindl, carried The cold tolerance of high ilexrotunda, then 3 generation seeds are carried out breeding to cultivate in kind that ilexrotunda seed must be hybridized, above-mentioned hot-house culture Temperature is 4-8 DEG C, cold resistance hybrid seed is screened, carbon dioxide volumetric concentration is 0.12%-0.14%;

(5)Cultivate:Ilexrotunda seed will be hybridized through step(3)Greenhouse cultivation is carried out after processing, the plant cultivated can be pushed away Extensive farming is planted.

Step(1)Described low temperature is baked, and its temperature is 35-40 DEG C.

Step(2)Described liquor natrii hypochloritis, its mass concentration are 50ppm.

Step(2)Described plant extraction liquid, its compound method are:

Purslane 14-18 parts, ganoderma lucidum 12-15 parts, Chinese holly fruit 7-10 parts, daffodil 5-8 parts are weighed according to quality meter part, after crushing Mill repeatedly 2 times, add the water of 6-8 times of gross mass, the boiling 3-4h under 52-56 DEG C, 2-3Mpa pressure, be concentrated into after filtering The 1/4 of original volume, the plant extraction liquid of system.

Step(3)Described ammoniated treatment, it is passed through ammonification pond body product 7%-9% ammonia, 34-37 DEG C of temperature, pressure 0.5-0.7Mpa。

Step(3)Described intermittent warming, its parameter are:17-21min is first preserved under the conditions of 5-8 DEG C, then in 28-32 12-14min is preserved under the conditions of DEG C.

The present invention has advantages below compared with prior art:Pollen is preserved, and pollen is gathered by female parent of excellent llex cornuta lindl It can keep pollen that there is preferable inhereditary feature, low temperature, which is baked, reduces moisture and cytoactive in pollen, can extend pollen Shelf lives;And nitrogen is filled with into conical flask and plays insulation, moistureproof and reduction cell metabolism horizontal force, can be to flower under low temperature Powder is preserved for a long time, and has higher activity.Hybridizing method, pollen will be preserved and carry out activation process, pollen can be awarded Higher activity is kept during powder, improves pollination probalility of success, liquor natrii hypochloritis and plant extract are used before being pollinated to ilexrotunda Liquid sprays to female Chinese ilex, and can reduce microorganism influences on pollination, can containing various plants active component in plant extraction liquid The activity of Chinese ilex pollen is improved, improves pollination success rate.Seed treatment, by 1 generation seed respectively through ammonification and intermittent warming, ammonification Processing can carry out sofening treatment to seed rigid shell, improve the germination percentage of seed, and intermittent warming can improve seed to temperature Tolerance, improve its resistance ability to external environment.Repeat hybridize, using llex cornuta lindl pollen to 1 generation seed with And 2 pollination hybridization is repeated in seed after hybridization, and ilexrotunda can be made to obtain the stable excellent inhereditary feature of llex cornuta lindl, improved The cold tolerance of ilexrotunda;Ilexrotunda after can making hybridization is bred in carrying out kind has more stable genetic characteristicses, follow-up Hereditary capacity is stable in plantation;Using low temperature high carbon dioxide environment culture, ilexrotunda cold resistance after hybridization can be cultivated Screening, and improve the speed of growth of plant.

Embodiment

Embodiment 1:

A kind of hybridizing method for improveing ilexrotunda winter resistance, comprises the following steps:

(1)Pollen preserves:In April-May using excellent llex cornuta lindl plant as female parent, it can keep pollen that there is preferable heredity Shape, male pollen is collected using sterilizing conical flask, nitrogen is filled with into conical flask after low temperature baking 17min protects under the conditions of 3 DEG C Deposit, reduce moisture and cytoactive in pollen, be filled with nitrogen and play insulation, moistureproof and reduction cell metabolism horizontal force, Pollen can be preserved for a long time, obtain preservation pollen;

(2)Hybridization:Pollen will be first preserved in Second Year March to April and carries out activation process, be improved the activity of pollen, be selected the iron winter Blue or green female plants pollination, in the morning 8 during pollination:00-9:00 respectively using liquor natrii hypochloritis and plant extract solution to Chinese ilex Flower sprinkling, can reduce microorganism is influenceed on pollination, and Chinese ilex pollen can be improved containing various plants active component in plant extraction liquid Activity, improve pollination success rate, dip in activation pollen using sterilizing hairbrush after natural air drying and pollinated, pollination 1 time daily, even It is continuous to carry out 3 days, 1 generation seed is obtained after culture;

(3)Seed treatment:1 generation seed is put into ammonification pond ammoniated treatment 76min, emollescence is played to kind of a shell, improves seed Germination percentage, seed is then immersed into plant extraction liquid again and carries out intermittent warming 15h, seed is improved to the tolerance of temperature, carries Its high resistance ability to external environment, must handle 1 generation seed;

(4)Repeat to hybridize:According to step(2)Method, 1 generation seed of processing and llex cornuta lindl hybridization are cultivated into obtain 2 generation seeds, 2 generations Seed cultivates to obtain 3 generation seeds with llex cornuta lindl hybridization again, and ilexrotunda can be made to obtain the stable excellent inhereditary feature of llex cornuta lindl, carried The cold tolerance of high ilexrotunda, then 3 generation seeds are carried out breeding to cultivate in kind that ilexrotunda seed must be hybridized, above-mentioned hot-house culture Temperature is 5 DEG C, and cold resistance hybrid seed is screened, and carbon dioxide volumetric concentration is 0.13%;

(5)Cultivate:Ilexrotunda seed will be hybridized through step(3)Greenhouse cultivation is carried out after processing, the plant cultivated can be pushed away Extensive farming is planted.

Step(1)Described low temperature is baked, and its temperature is 36 DEG C.

Step(2)Described liquor natrii hypochloritis, its mass concentration are 50ppm.

Step(2)Described plant extraction liquid, its compound method are:

15 parts of purslane, 13 parts of ganoderma lucidum, 8 parts of Chinese holly fruit, 6 parts of daffodil are weighed according to quality meter part, mills 2 after crushing repeatedly It is secondary, the water of 6.5 times of gross mass is added, the boiling 3.5h under 53 DEG C, 2.3Mpa pressure, the 1/4 of original volume is concentrated into after filtering, The plant extraction liquid of system.

Step(3)Described ammoniated treatment, it is passed through the ammonia of ammonification pond body product 7.2%, 35 DEG C of temperature, pressure 0.54Mpa。

Step(3)Described intermittent warming, its parameter are:18min is first preserved under the conditions of 6 DEG C, then under the conditions of 29 DEG C Preserve 13min.

Embodiment 2:

(1)Pollen preserves:In April-May using excellent llex cornuta lindl plant as female parent, it can keep pollen that there is preferable heredity Shape, male pollen is collected using sterilizing conical flask, nitrogen is filled with into conical flask after low temperature baking 18min protects under the conditions of 4 DEG C Deposit, reduce moisture and cytoactive in pollen, be filled with nitrogen and play insulation, moistureproof and reduction cell metabolism horizontal force, Pollen can be preserved for a long time, obtain preservation pollen;

(2)Hybridization:Pollen will be first preserved in Second Year March to April and carries out activation process, be improved the activity of pollen, be selected the iron winter Blue or green female plants pollination, in the morning 8 during pollination:00-9:00 respectively using liquor natrii hypochloritis and plant extract solution to Chinese ilex Flower sprinkling, can reduce microorganism is influenceed on pollination, and Chinese ilex pollen can be improved containing various plants active component in plant extraction liquid Activity, improve pollination success rate, reduce and dip in activation pollen using sterilizing hairbrush after natural air drying and pollinated, pollinate 1 daily It is secondary, it is carried out continuously 3 days, 1 generation seed is obtained after culture;

(3)Seed treatment:1 generation seed is put into ammonification pond ammoniated treatment 79min, emollescence is played to kind of a shell, improves seed Germination percentage, seed is then immersed into plant extraction liquid again and carries out intermittent warming 16h, seed is improved to the tolerance of temperature, carries Its high resistance ability to external environment, must handle 1 generation seed;

(4)Repeat to hybridize:According to step(2)Method, 1 generation seed of processing and llex cornuta lindl hybridization are cultivated into obtain 2 generation seeds, 2 generations Seed cultivates to obtain 3 generation seeds with llex cornuta lindl hybridization again, and ilexrotunda can be made to obtain the stable excellent inhereditary feature of llex cornuta lindl, carried The cold tolerance of high ilexrotunda, then 3 generation seeds are carried out breeding to cultivate in kind that ilexrotunda seed must be hybridized, above-mentioned hot-house culture Temperature is 6 DEG C, and cold resistance hybrid seed is screened, and carbon dioxide volumetric concentration is 0.14%;

(5)Cultivate:Ilexrotunda seed will be hybridized through step(3)Greenhouse cultivation is carried out after processing, the plant cultivated can be pushed away Extensive farming is planted.

Step(1)Described low temperature is baked, and its temperature is 39 DEG C.

Step(2)Described liquor natrii hypochloritis, its mass concentration are 50ppm.

Step(2)Described plant extraction liquid, its compound method are:

17 parts of purslane, 14 parts of ganoderma lucidum, 9 parts of Chinese holly fruit, 7 parts of daffodil are weighed according to quality meter part, mills 2 after crushing repeatedly It is secondary, the water of 7.5 times of gross mass is added, the boiling 4h under 55 DEG C, 2.7Mpa pressure, the 1/4 of original volume is concentrated into after filtering, system Plant extraction liquid.

Step(3)Described ammoniated treatment, it is passed through the ammonia of ammonification pond body product 8.5%, 36 DEG C of temperature, pressure 0.68Mpa。

Step(3)Described intermittent warming, its parameter are:20min is first preserved under the conditions of 7 DEG C, then under the conditions of 31 DEG C Preserve 14min.

Contrast 1:

This contrast 1 does not carry out step compared with embodiment 1(1)Middle nitrogen uses, and other steps are same as Example 1.

Contrast 2:

This contrast 2 does not carry out step compared with embodiment 1(2)Middle pollinating method, other steps are same as Example 1.

Contrast 3:

This contrast 3 does not carry out step compared with embodiment 1(3)Middle ammoniated treatment, other steps are same as Example 1.

Contrast 4:

This contrast 4 does not carry out step compared with embodiment 2(3)Middle intermittent warming, other steps are same as Example 2.

Contrast 5:

This contrast 5 does not carry out step compared with embodiment 2(4)Repeat to hybridize, other steps are same as Example 2.

Control group:

Control group is planted with normal ilexrotunda seed, is not carried out nitrogen and is hybridized using, pollination, ammonification, the repetition that relents.

To embodiment 1, embodiment 2, contrast 1, contrast 2, contrast 3, contrast 4, contrast 5 and control group experimental program, statistics Germination percentage, cold resistance, pollination success rate and the time at florescence of ilexrotunda.

Experimental data:

Project Germination percentage % Cold resistance DEG C Success rate of pollinating % Time at florescence d Embodiment 1 93.1 -9.0 81.8 34 Embodiment 2 92.5 -8.5 82.4 32 Contrast 1 93.5 -9.0 72.4 34 Contrast 2 92.8 -8.5 69.7 33 Contrast 3 76.0 -9.0 82.1 35 Contrast 4 83.9 -6.5 82.5 26 Contrast 5 92.7 -5.0 81.5 29 Control group 67.4 -3.5 0.0 25

Synthesis result:The ilexrotunda that the present invention is cultivated, compared with control group, ilexrotunda germination percentage can improve 25.7%, cold resistance 5.5 DEG C are improved, pollination success rate improves 21.6% more than 81.8%, compared with conventional method, ilexrotunda florescence time lengthening 9d.Using Pollen preserves and pollinating method, and it is 9.4%, 12.1% that can improve pollination success rate;Using ammoniated treatment method, germination percentage can be improved 17.1%;And Temp change method is used, germination percentage 8.6% can be improved, cold resistance improves 2 DEG C, florescence time lengthening 6d;It is miscellaneous using repeating Friendship method, 3.5 DEG C of cold resistance, florescence time lengthening 3d can be improved.

Claims (6)

1. a kind of hybridizing method for improveing ilexrotunda winter resistance, it is characterised in that comprise the following steps:
(1)Pollen preserves:In April-May using excellent llex cornuta lindl plant as female parent, male pollen is collected using sterilizing conical flask, Nitrogen is filled with into conical flask to preserve under the conditions of 2-5 DEG C, obtain preservation pollen after low temperature baking 16-19min;
(2)Hybridization:Pollen will be first preserved in Second Year March to April and carries out activation process, select the pollination of ilexrotunda female plants, In the morning 8 during pollination:00-9:00 is sprayed using liquor natrii hypochloritis and plant extract solution to Chinese ilex respectively, natural air drying Dip in activation pollen using sterilizing hairbrush afterwards to be pollinated, pollination 1 time, is carried out continuously 3 days daily, and 1 generation seed is obtained after culture;
(3)Seed treatment:1 generation seed is put into ammonification pond ammoniated treatment 75-80min, seed is then immersed into plant extraction liquid Intermittent warming 14-17h is carried out, 1 generation seed must be handled;
(4)Repeat to hybridize:According to step(2)Method, 1 generation seed of processing and llex cornuta lindl hybridization are cultivated into obtain 2 generation seeds, 2 generations Seed cultivates to obtain 3 generation seeds with llex cornuta lindl hybridization again, then 3 generation seeds is carried out breeding to cultivate in kind that ilexrotunda kind must be hybridized Son, above-mentioned hot-house culture temperature are 4-8 DEG C, and carbon dioxide volumetric concentration is 0.12%-0.14%;
(5)Cultivate:Ilexrotunda seed will be hybridized through step(3)Greenhouse cultivation is carried out after processing, the plant cultivated can be pushed away Extensive farming is planted.
2. the hybridizing method of improvement ilexrotunda winter resistance as claimed in claim 1, it is characterised in that step(1)Described is low Temperature is baked, and its temperature is 35-40 DEG C.
3. the hybridizing method of improvement ilexrotunda winter resistance as claimed in claim 1, it is characterised in that step(2)Described time Sodium chlorate solution, its mass concentration are 50ppm.
4. the hybridizing method of improvement ilexrotunda winter resistance as claimed in claim 1, it is characterised in that step(2)Described plant Thing extract solution, its compound method are:
Purslane 14-18 parts, ganoderma lucidum 12-15 parts, Chinese holly fruit 7-10 parts, daffodil 5-8 parts are weighed according to quality meter part, after crushing Mill repeatedly 2 times, add the water of 6-8 times of gross mass, the boiling 3-4h under 52-56 DEG C, 2-3Mpa pressure, be concentrated into after filtering The 1/4 of original volume, the plant extraction liquid of system.
5. the hybridizing method of improvement ilexrotunda winter resistance as claimed in claim 1, it is characterised in that step(3)Described ammonia Change is handled, and it is passed through ammonification pond body product 7%-9% ammonia, 34-37 DEG C of temperature, pressure 0.5-0.7Mpa.
6. the hybridizing method of improvement ilexrotunda winter resistance as claimed in claim 1, it is characterised in that step(3)Described change Temperature processing, its parameter are:17-21min is first preserved under the conditions of 5-8 DEG C, then 12-14min is preserved under the conditions of 28-32 DEG C.
CN201710987694.6A 2017-10-21 2017-10-21 A kind of hybridizing method for improveing ilexrotunda winter resistance CN107711490A (en)

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Publication number Priority date Publication date Assignee Title
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US20170231140P1 (en) * 2016-02-09 2017-08-10 Jack Mitchell Magee Ilex plant named 'EN1'

Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
CN102986525A (en) * 2012-12-31 2013-03-27 江苏省中国科学院植物研究所 Crossbreeding method for hollies
US20170231140P1 (en) * 2016-02-09 2017-08-10 Jack Mitchell Magee Ilex plant named 'EN1'

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ZHANG DONG-LIN等: "cross compatibility of Ilex glabra(L.) A.Gray with Ilex× meserveae S.Y.Hu and Ilex verticillata (L.) A.Gray", 《中南林业科技大学学报》 *
张金兰等: "氨化处理对假高粱等杂草种子灭活机制的分析研究", 《植物检疫》 *
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Application publication date: 20180223