CN107996231A - Film and preparation method thereof is invaded in a kind of natural resistance of prevention and control rice sheath blight disease - Google Patents
Film and preparation method thereof is invaded in a kind of natural resistance of prevention and control rice sheath blight disease Download PDFInfo
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- CN107996231A CN107996231A CN201711228212.5A CN201711228212A CN107996231A CN 107996231 A CN107996231 A CN 107996231A CN 201711228212 A CN201711228212 A CN 201711228212A CN 107996231 A CN107996231 A CN 107996231A
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- film
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
- A01G13/02—Protective coverings for plants; Coverings for the ground; Devices for laying-out or removing coverings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
- A01G2013/004—Liquid mulch
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Film and preparation method thereof is invaded in a kind of natural resistance the invention discloses prevention and control rice sheath blight disease, and following one or more combination is included at least in its raw material components：Water soluble chitosan, guar gum, sodium benzoate, nano silicon dioxide, organosilicon.The film is using water soluble chitosan and guar gum as main film forming substance, and two kinds of materials are natural products, to environment and human body nonhazardous.The present invention achievees the purpose that to prevent Rhizoctonia solani Kuhn from invading, while can reduce the usage amount of chemical pesticide, be conducive to environmental protection and food security by forming one layer of sightless film of naked eyes on rice cane and blade.
Description
Technical field
Film is invaded in a kind of natural resistance the present invention relates to prevention and control rice sheath blight disease, which sprays after rice,
Its surface forms layer protecting film, while rice normal growth and development is not influenced, protects rice not by Rhizoctonia solani Kuhn
Intrusion, can also reduce the use of chemical pesticide, environmental protection, water source and rice safety.Belong to harmful organism green prevention neck
Domain.
Background technology
Rice is a kind of important grain product, has the population of nearly half in the world using rice as staple food.The disease of rice
Insect pest can cause the rice underproduction, be the main reason for influencing the yield of rice.
Rice sheath blight disease belongs to one of three major disease of rice, occurs in China extremely widespread.Whether occurrence frequency, generation
Area or caused by damage losses banded sclerotial blight occupy first of rice disease.Especially in South Rice Region of China, since how fertile dense planting is, line
Blight tends to occur seriously, the rice underproduction 50% can be caused when being in a bad way.Traditional rice sheath blight disease controlling technology is mainly
Chemical pesticide control, but it is big to environmental hazard, is also easy to produce the drawbacks of resistant strain also becomes increasingly conspicuous.
Plant protection membrane technology is a kind of new plant protection technology of rising in recent years, is protected compared to traditional plant
Shield technology, plant-protecting film have the advantages that ecological, environmental protective, easy and effective.The principle of plant-protecting film be using plant self
Protection.Almost all of plant outer surface all has the cuticula of " the natural protection coat " that is referred to as plant.Land plant
Leaf surfaces there is one layer of cutin membrane, it is mainly made of wax and hydrophobic cutin, becomes plant and external environment
Interface, the outermost wax coat of cutin membrane, can prevent the intrusion of pathogen.Plant protection membrane technology is in rice sheath blight disease
Prevention in application for rice pest research provide a kind of new thinking, tool is of great significance.
In recent years, with attention of the people to environment and own health, natural materials are just gradually in the fields such as food, medical treatment
Substitution synthetic material gradually.Most of these natural materials have the features such as good film-forming property, good permeability, and energy plant surface is formed
One layer of thin pellicle, this tunic change the structure of plant surface, and then can influence the knowledge of pathogen or insect to plant
Not, the parasitism of pathogen, the feeding of insect, oviposition are influenced.
But at present in the art there has been no on by plant protection membrane technology come the technology of prevention and control rice sheath blight disease
Scheme discloses.
The content of the invention
The purpose of the present invention is the preventing and controlling pesticide dosage for current rice sheath blight disease is big, drug-fast strain produces etc.
Problem, there is provided film and preparation method thereof is invaded in a kind of natural resistance for preventing rice sheath blight disease, mainly selected water soluble chitosan,
The basic material of the food-grades such as guar gum, made natural resistance are invaded film and are sprayed in rice plant surface, can prevent rice
Banded sclerotial blight, reduces the usage amount of pesticide, reduces environmental pollution.
The present invention mainly employs following technical scheme：
Film is invaded in a kind of natural resistance of prevention and control rice sheath blight disease of the present invention, is included at least in its raw material components with next
Kind or several combinations：Water soluble chitosan, guar gum, sodium benzoate, nano silicon dioxide, organosilicon.
Wherein, by weight, it is by 6 parts of water soluble chitosan, 1 part of guar gum, 1 part of sodium benzoate, nano-silica
1 part of SiClx, 1 part of organosilicon, 1000-16000 parts of water are made.
The preparation method of film is invaded in the natural resistance of this prevention and control rice sheath blight disease, is included the following steps：
Step 1：Film forming matter water soluble chitosan and guar gum are dissolved in the water, obtain film forming glue；
Step 2：Sodium benzoate is dissolved in the solution that step 1 obtains, glue is stirred at normal temperatures；
Step 3：By nano silicon dioxide:Add in the solution that step 2 obtains, glue is stirred at normal temperatures fully, surpassed
Obtained after sound degassing spare；
Step 4：Will:Organosilicon is added in the solution that step 3 obtains, and is stirred evenly, and is obtained naturally resistance and is invaded film.
Wherein, it is stirred in step 2 and step 3 using magnetic stirring apparatus.Mixing time is 30-60 minutes in step 2.
The chitosan that the present invention uses is the deacetylated product of chitin, is a kind of white or flaxen translucent solid
Body, since it is nontoxic, is easily biodegradable, and there are catabolic enzyme in human body, in addition its with good film forming, thickening property,
Hygroscopicity, have certain bacteriostatic activity, and induced resistance, can preferably suppress polyphenol oxidase activity after forming film, improves
Peroxidase activity is to reach fresh-keeping effect.The guar gum that the present invention uses is extracted from cluster bean endosperm, is one
The non-ionic sweet glycan of gala of kind.For white or fawn powder, be stickiness maximum in nature material, have well
Water solubility.
Therefore, it is main film forming substance that film of the invention, which selects natural materials water soluble chitosan and guar gum, is added
Add some auxiliary substances to be allowed to certain gas permeability, ductility, viscosity, spray and form one layer of naked eyes not in rice plant surface
Visible film layer, the film layer resistance of rainwater washing against, it is wrinkle resistant, do not peel off, hinder the intrusion of Rhizoctonia solani Kuhn, reach prevention rice
The purpose of banded sclerotial blight.Main matter in this film is nontoxic or less toxic, on the premise of rice normal growth and development is ensured,
Rice sheath blight disease can be prevented, pollution of the pesticide to environment is reduced, ensures grain security and health.It is nuisanceless to keeping the safety in production
Food is of great significance.
Brief description of the drawings
Inhibitory action schematic diagram of the film to Rhizoctonia solani Kuhn is invaded in the natural resistance that Fig. 1 is the present invention.
Embodiment
Technical scheme is clearly and completely described below in conjunction with embodiment.The numerical value that is directed to,
Number or ratio are mass figures, mass fraction or mass ratio such as without mark.
The present invention is implemented：
Embodiment 1：
The preparation method of film is invaded in the resistance of the present invention：By weight, by 6 parts of film forming matter water soluble chitosan and melon
You are dissolved in 100 parts of water 1 part of bean gum, obtain film forming glue.Sodium benzoate is dissolved in film forming glue for 1 part, is stirred with magnetic force
Device is mixed to stir glue at normal temperatures 30-60 minutes.1 part of nano silicon dioxide is added in solution, with magnetic stirring apparatus by glue
Liquid stirs fully at normal temperatures, ultrasound degassing after ten minutes it is spare.1 part of organosilicon is added, is stirred evenly.Finally obtain natural
Film is invaded in resistance.
Experimental example 1：
To understand the indoor biological of inventive film agent activity, and scientific basis is provided to promote and apply in the future, therefore use bacterium
Silk growth rate method, verifies concentration EC in the suppression of film50.By film be diluted to 200 times, 400 times, 800 times, 1600 times,
3200 times of liquid, using clear water as control, dilution film liquid and clear water equivalent are applied on AEA tablets, after drying, in colony edge
Beat and take a diameter of 5mm Rhizoctonia solani Kuhns bacteria cake, be inoculated on the AEA plate centers position of series concentration containing film and medicament,
Each processing sets 6 repetitions.After 7-10 days, colony diameter is measured using crossing method, each bacterium colony bacterium is calculated using formula (1)
Silk growth inhibition ratio.Logarithm (x) by Excel softwares to each concentration for the treatment of, the probit value (y) for suppressing growth are linearly returned
Return analysis, calculate virulence regression equation and correlation coefficient r value, be inhibited middle concentration EC50。
From experimental result, growth of this film to Rhizoctonia solani Kuhn mycelia has certain inhibitory action, it suppresses
Rate is between 25%-80%, EC50For 463.6mg/L.Inhibitory action of the film to Rhizoctonia solani Kuhn is invaded in the natural resistance of the present invention
Schematic diagram is as shown in Figure 1.
Experimental example 2：
To understand the indoor prevention effect of this film, measured by pot experiment, the indoor preventive effect of this film.
Rice strain processing：Using tillering stage rice as experimental vehicle, wherein tillering stage rice is cultivated for crop field, treats its length to tiller
Phase, uproops, and cleans, dries, 15 plants one group, is compareed plus clear water, accomplishes fluently rice after drying on the cystosepiment of sky and is used in combination
Stalk is fixed and is finally placed in length × width × height=30cm × 20cm equipped with 0.2 times of nutrient solution × 15cm plastic tubs by absorbent cotton
In.This film and mixture 1 (0.96 part of difenoconazole is added in film) are diluted to 100 times, 200 times, 400 times, 800 times
And 1600 times of film liquid, spray on rice plant, every plant of quantitative 3mL.By cultured Rhizoctonia solani Kuhn card punch
The bacteria cake of 5mm is broken into, the rice root being connected to after each strain spray film, every plant connects 2 bacteria cakes.It is placed in per basin rice with plastic foil moisturizing
On supporter after processing.
Bacterial strain processing：Using AEA culture mediums, its formula is dusty yeast 5g, KCl 0.5g, MgSO40.25g, KH2PO4
1.5g, NaNO36g, glycerine 20mL, agar powder 20g, deionized water 1000mL.Fungus block is seeded to AEA plate centers, is put
Dark culturing 5-7 days in 28 DEG C of constant incubators.
Record data：The rice pathogenesis situation after 3d, 5d, 7d, including morbidity strain number and severity are recorded, according to public affairs
Formula (2)-(3) calculate its disease index
Disease index after CK------ blank control dispensers
Disease index after PT------ chemicals treatment dispensers
Table 2 is referred to the preventive effect of rice sheath blight disease.The preventive effect that film 200 times of liquid of dilution are invaded in resistance is optimal, average anti-
Effect up to 73.91%, resistance, which invades film and dilutes 200 times of liquid, be averaged preventive effect and jinggangmeisu, the addition common dose of difenoconazole
Quite.The preventive effect diluted in mixture 1 after 100 times is optimal, and 5 days average preventive effects reach 85.51% after inoculation, difenoconazole
Average preventive effect be 75.36% significant difference.7 days after inoculation, the average preventive effect of 200 times of the dilution of mixture 1 is significantly higher than resistance and invades film
200 times of liquid are diluted, the average preventive effect difference of the field recommended dose of difenoconazole is not notable.Therefore, film is invaded in natural resistance has
Certain indoor prevention effect, and addition conventional dose can effectively improve preventive effect, greatly reduce the dosage of medicament, and extend
The lasting period of film.
Experimental example 3：
To understand the indoor therapeutic effect of this film, the indoor therapeutic effect of this film is measured by pot experiment.
Rice strain processing：Using tillering stage rice as experimental vehicle, wherein tillering stage rice is cultivated for crop field, treats its length to tiller
Phase, uproops, and cleans, dries, 15 plants one group, is compareed plus clear water, and each processing sets 3 groups of repetitions, and rice is mounted in and is accomplished fluently
On the cystosepiment in hole, and with absorbent cotton by stalk fix it is last be placed in length × width × height=30cm equipped with 0.2 times of nutrient solution ×
In 20cm × 15cm plastic tubs.Cultured Rhizoctonia solani Kuhn is broken into the bacteria cake of 5mm with card punch, is connected to each strain rice root
Portion, every plant connects 2 bacteria cakes.It is placed in per basin rice on the supporter after being handled with plastic foil moisturizing, after placing 3-4 days, treats that CK plants
Strain is all susceptible, by this film and mixture 1 (0.96 part of difenoconazole is added in film) be diluted to 100 times, 200 times, 400
Again, 800 times and 1600 times of film liquid, sprays on rice plant, every plant quantifies 3mL.
Bacterial strain processing：Using AEA culture mediums.Fungus block is seeded to AEA plate centers, is placed in black in 28 DEG C of constant incubators
Light culture 5-7 days.
Record data：The rice pathogenesis situation after 3d, 5d, 7d, including morbidity strain number and severity are recorded, according to public affairs
Formula (5)-(6) calculate its disease index and preventive effect.Rice sheath blight disease severity grade scale such as table 1.
In formula：CK0Disease index before --- --- blank control dispenser；CK1Disease index after --- --- blank control dispenser；
PT0Disease index before --- --- chemicals treatment dispenser；PT1Disease index after --- --- chemicals treatment dispenser.
Table 3 is referred to the preventive effect of rice sheath blight disease.The therapeutic effect that resistance is invaded in film after 200 times of dilution is optimal, sprays film
5 days average preventive effects are 49.06% afterwards, are significantly higher than the field recommended dose of difenoconazole.The therapeutic effect of mixture 1 is with dilution
The increase of multiple and reduce, with the increase of time, therapeutic effect first increases to be reduced afterwards.The treatment after 100 times is diluted in mixture 1 to make
With optimal, 5 days average preventive effects reach 51.99% after spraying film, be significantly higher than resistance invade film dilute 200 times it is flat with field recommended dose
Equal preventive effect；The therapeutic effect that film 200 times of liquid of dilution is invaded in resistance dilutes 200 times of liquid height compared with mixture 1, but difference is not notable.Therefore, naturally
Film is invaded in resistance certain indoor therapeutic effect, and addition conventional dose can effectively improve preventive effect, greatly reduce medicament
Dosage.
Experimental example 4：
To understand influence of the inventive film agent to rice growth, choose similar in growing way, with point of suspension culture
Tiller phase rice seedlings are experimental subjects, by 5 concentration of inventive film dilution agent, be respectively 100 times, 200 times, 400 times, 800 times and
1600 times, clear water compares, and totally 6 processing, quantitative dilute solution are sprayed on rice cane with miniaturised nebuliser, each
Processing sets 3 repetitions, and rice seedlings plant height, adventitious root root long and the adventitious root radical newly grown were recorded at the three, the five, seven days
(the taken repetition of experiment is different every time, therefore each data are only contrasted with same day measurement data).
Test result indicates that spraying film of the present invention, the plant height, adventitious root root long and adventitious root radical difference to rice are not
Greatly, illustrate that the film does not have too much influence to the rice growth of rice seedlings.
To understand addition preventive effect of the inventive film agent to rice sheath blight disease, and scientific basis is provided to promote and apply from now on,
2016 using film of the present invention and in Guizhou Province Huangping County Jiu Zhou towns progress field trial (rice varieties：Excellent No. 3 of honor；Spray
Day with fog：Board agricultural hand air-blast atomizer, model SX-CS8A under city), and with 10% difenoconazole WDG 60g/hm2、
The solvable pulvis 50g/hm of 20% jinggangmeisu2Contrast.
Experiment carries out in Huangping County Jiu Zhou towns, and film and medicine film mixture are invaded in natural resistance, and (mixture 1 adds 0.96 part in film
Difenoconazole and mixture 2 add 0.33 part of jinggangmeisu in film) it is diluted to 200 times, 400 times, 800 times.Including 12
Handle, 3 repetitions, totally 36 cells.Respectively at before spraying film, spray film after 1 day, 3 days, 5 days, 7 days to every cell carry out diagonal
5 points of samplings, every investigation are connected 5 clumps, totally 25 clumps, and record total strain number, diseased plant number and sick series, measure each cell rice line
Blight disease index, calculates preventive effect.
As can be seen from Table 5, resistance, which is invaded film and is diluted to after 200 times, can reach the field control effect of rice sheath blight disease
66.85%, preventive effect is up to 75.59% after mixture 1 is diluted to 200 times, and preventive effect is 69.33% after mixture 2 dilutes 200 times, mixture 1,2
Preventive effect is higher than single dose difenoconazole and jinggangmeisu, and the preventive effect that film is invaded in resistance is significantly higher than jinggangmeisu.Mixture 1 dilutes 200 times
Liquid be averaged preventive effect and other concentration differences it is notable, be significantly higher than difenoconazole and resistance is used alone to invade film and dilute 200 times of liquid；It is mixed
Agent 2 dilutes 200 times of liquid preventive effect that be averaged and jinggangmeisu and resistance is relatively used alone invades film 200 times of liquid of dilution, and significant difference.Thus may be used
Infer, 3 kinds of formulas have rice sheath blight disease certain control effect, can effectively reduce making for difenoconazole and jinggangmeisu
With.
The Rhizoctonia solani Kuhn of the present invention naturally invade in the formula of film by resistance, and water soluble chitosan and guar gum are film forming
Agent, Nano carbon white increase particulate matter in film, and organosilicon makes protective film have certain ductility on rice.Control efficacy
Show as 200 times of 100 times of dilution and dilution preferably, average preventive effect is respectively 73.91% and 62.32%.Field efficiency test table
Now preferable for 200 times and 400 times of dilution, average preventive effect is 66.85% and 63.73%, and after adding conventional pesticide, preventive effect significantly carries
Height increase.It can be seen that film of the present invention, which is used alone, has rice sheath blight disease certain preventive effect from this data, after adding pesticide
Using preventive effect can also be increased.Non- murder by poisoning its stomach and nervous system of the present invention, the problem of without poisoning and drug-fast problem,
On the premise of not influencing rice normal growth and development, Rice Cropping non agricultural chemical residuum is solved the problems, such as.
1 rice sheath blight disease severity of table is classified
Table 1Severity classification of rice sheath blight
Table 2 hinders the prevention effect for invading film with mixture 1 to rice sheath blight disease
Table2Preventive effect of invasion film and mix1on Rice Sheath
Blight
Note：With after column of figure, different letters represent to show using difference ` between DuncanShi duncan's new multiple range methods inspection numerical value in table
Write (p>0.05).
Note:Data in the same column followed by different letters are
significantly different by Duncan’s new multiple range test(p>0.05)。
Film and therapeutic effect of the mixture 1 to rice sheath blight disease are invaded in the resistance of table 3
Table3Effect of invasion film and mix1on Rice Sheath Blight
Note：With after column of figure, different letters represent to show using difference ` between DuncanShi duncan's new multiple range methods inspection numerical value in table
Write (p>0.05).
Note:Data in the same column followed by different letters are
significantly different by Duncan’s new multiple range test(p>0.05)。
Table 4 sprays resistance and invades influence of the film to tillering stage Plant Height of Rice, adventitious root root long and adventitious root radical
Table 4The effect on the rice plant height,the length and the number
of adventitious roots affter spraying invasion film
Note：With after column of figure, different letters represent to show using difference ` between DuncanShi duncan's new multiple range methods inspection numerical value in table
Write (p>0.05).
Note:Data in the same column followed by different letters are
significantly different by Duncan’s new multiple range test(p>0.05)。
Field efficacy of 5 Rhizoctonia solani Kuhn of table to rice sheath blight disease
Table5The control effect of Rhizoctonia solani anti invasion film on
Rice Sheath Blight in field
Note：With after column of figure, different letters represent to show using difference ` between DuncanShi duncan's new multiple range methods inspection numerical value in table
Write (p>0.05).
Note:Data in the same column followed by different letters are
significantly different by Duncan’s new multiple range test(p>0.05)。
Certainly, the above is the concrete application example of the present invention, and the present invention also has other embodiments, all using equivalent
The technical solution that replacement or equivalent transformation are formed, all falls within protection domain of the presently claimed invention.
Claims (5)
1. film is invaded in a kind of natural resistance of prevention and control rice sheath blight disease, it is characterised in that：Included at least in its raw material components following
One or more combination：Water soluble chitosan, guar gum, sodium benzoate, nano silicon dioxide, organosilicon.
2. film is invaded in the natural resistance of prevention and control rice sheath blight disease according to claim 1, it is characterised in that：By weight,
It is by 6 parts of water soluble chitosan, 1 part of guar gum, 1 part of sodium benzoate, 1 part of nano silicon dioxide, 1 part of organosilicon, water 1000-
16000 parts are made.
3. the preparation method of film is invaded in a kind of natural resistance of prevention and control rice sheath blight disease as claimed in claim 1 or 2, its feature exists
In including the following steps：
Step 1：Film forming matter water soluble chitosan and guar gum are dissolved in the water, obtain film forming glue；
Step 2：Sodium benzoate is dissolved in the solution that step 1 obtains, glue is stirred at normal temperatures；
Step 3：By nano silicon dioxide:Add in the solution that step 2 obtains, glue is stirred at normal temperatures fully, ultrasound is de-
Obtained after gas spare；
Step 4：Will:Organosilicon is added in the solution that step 3 obtains, and is stirred evenly, and is obtained naturally resistance and is invaded film.
4. the preparation method of film is invaded in the natural resistance of prevention and control rice sheath blight disease according to claim 3, it is characterised in that：Step
Rapid 2 and step 3 in be stirred using magnetic stirring apparatus.
5. the preparation method of film is invaded in the natural resistance of prevention and control rice sheath blight disease according to claim 4, it is characterised in that：Step
Mixing time is 30-60 minutes in rapid 2.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711228212.5A CN107996231A (en) | 2017-11-29 | 2017-11-29 | Film and preparation method thereof is invaded in a kind of natural resistance of prevention and control rice sheath blight disease |
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| CN107996231A true CN107996231A (en) | 2018-05-08 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115259936A (en) * | 2022-06-27 | 2022-11-01 | 大连益丰农生物科技有限公司 | Gelatin selenium peptide-chitin composite plant protection spraying film agent and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105766912A (en) * | 2016-03-25 | 2016-07-20 | 台州市农业科学研究院 | Chitosan preparation and application thereof in preventing and controlling rice false smut |
| CN105820668A (en) * | 2016-05-27 | 2016-08-03 | 贵州大学 | HZSN.J absorption-resistant film agent for preventing and controlling rice planthoppers and preparation method thereof |
| CN106070209A (en) * | 2016-05-27 | 2016-11-09 | 贵州大学 | The citral HP β CD K sustained release film formulation of prevention and control rice blast |
-
2017
- 2017-11-29 CN CN201711228212.5A patent/CN107996231A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105766912A (en) * | 2016-03-25 | 2016-07-20 | 台州市农业科学研究院 | Chitosan preparation and application thereof in preventing and controlling rice false smut |
| CN105820668A (en) * | 2016-05-27 | 2016-08-03 | 贵州大学 | HZSN.J absorption-resistant film agent for preventing and controlling rice planthoppers and preparation method thereof |
| CN106070209A (en) * | 2016-05-27 | 2016-11-09 | 贵州大学 | The citral HP β CD K sustained release film formulation of prevention and control rice blast |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115259936A (en) * | 2022-06-27 | 2022-11-01 | 大连益丰农生物科技有限公司 | Gelatin selenium peptide-chitin composite plant protection spraying film agent and preparation method thereof |
| CN115259936B (en) * | 2022-06-27 | 2024-05-17 | 大连益丰农生物科技有限公司 | Gelatin selenopeptide-chitin composite plant protection spray film agent and preparation method thereof |
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Application publication date: 20180508 |