CN109221246A - A kind of long shoot trichoderma T6 bacterial strain wettable powder and its application - Google Patents
A kind of long shoot trichoderma T6 bacterial strain wettable powder and its application Download PDFInfo
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- CN109221246A CN109221246A CN201811100846.7A CN201811100846A CN109221246A CN 109221246 A CN109221246 A CN 109221246A CN 201811100846 A CN201811100846 A CN 201811100846A CN 109221246 A CN109221246 A CN 109221246A
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- bacterial strain
- wettable powder
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- shoot trichoderma
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- Agronomy & Crop Science (AREA)
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Abstract
The invention belongs to biological pesticide technical fields, and in particular to a kind of long shoot trichoderma T6 bacterial strain wettable powder and its application.The effective active composition of the wettable powder includes 10% long shoot trichoderma T6 bacterial strain conidial powder, additionally includes 0.6% wetting agent, 5% dispersing agent; 0.05% sticker; 0.1% conidia germination stimulus, 0.3% uv-protector, balance carriers talcum powder.The generation and harm of alternaria leaf spot of apple and apple anthrax leaf blight can be effectively prevented.
Description
Technical field
The invention belongs to biological pesticide technical fields, and in particular to a kind of long shoot trichoderma T6 bacterial strain wettable powder and its answer
With.
Background technique
In recent years, alternaria leaf spot of apple and apple anthrax leaf blight seriously affect the production of China's apple, mainly endanger
Evil Apple Leaves, can lead to fruit tree leaf after generation and largely fall off, seriously affect the normal growth, flower_bud formation, fruit of apple tree
The real yield and quality increased with coming year apple.When occurring serious, tree body can be caused to fall leaves in advance, shedding, fruit cannot be mature,
Effectively cause the underproduction and economic loss.Currently, both at home and abroad for alternaria leaf spot of apple and apple anthrax leaf blight prevention and treatment still
Using chemical bactericide as main control measure, but the use of chemical bactericide brings fruit quality decline and nuisance
Matter is exceeded and ecological disruption and the problems such as environmental pollution, has seriously affected food security and human health.Therefore, biology is anti-
It controls and can be used as a kind of environmentally friendly disease prevention techniques, be a kind of effective measures of current substitution chemical pesticide, it has also become
The hot spot studied both at home and abroad.Trichoderma (Trichoderma spp.) is a kind of widely distributed soil inhabitant.It is reported that trichoderma
Bacterium has preferable preventive and therapeutic effect to some soilborne fungal pathogens, especially domestic and international in relation to Trichoderma harzianum (T.hatzianum) and green
Color trichoderma (T.viride) is applied to vegetable disease prevention and treatment aspect and has more research and report, but about long shoot trichoderma T6 bacterium
The development of strain wettable powder and prevention and treatment alternaria leaf spot of apple and apple anthrax leaf blight etc. have not been reported.Meanwhile
Its biological and ecological methods to prevent plant disease, pests, and erosion activity how is kept in Trichoderma application process, improves biological and ecological methods to prevent plant disease, pests, and erosion vigor, Shelf-life and keeps field biocontrol effect
Stability etc. is the Main way of current urgent problem and research.Therefore, long shoot trichoderma T6 bacterial strain wettable powder is developed
Agent is to improve the effective way of microbial cells biocontrol effect, the prevention and treatment for alternaria leaf spot of apple and apple anthrax leaf blight
It is worth with important theory and practice.
Summary of the invention
Regarding to the issue above and the deficiencies in the prior art, the purpose of the present invention is to provide a kind of novel biological pesticide systems
Agent, i.e., a kind of long shoot trichoderma T6 bacterial strain wettable powder, in percentage by weight, the effective active composition of the wettable powder
Include 10% long shoot trichoderma T6 bacterial strain conidial powder.
Further, in percentage by weight, which further includes 0.6% wetting agent, 5% dispersing agent,
0.05% sticker, 0.1% conidia germination stimulus, 0.3% uv-protector, balance carriers talcum powder.
Further, the wetting agent is lauryl sodium sulfate, and concentration is 60 μ g/mL.
Further, the dispersing agent is sodium lignin sulfonate.
Further, the sticker is xanthan gum.
Further, the conidia germination stimulus is zinc sulfate, and concentration is 10 μ g/mL.
Further, the uv-protector is carbon black.
Further, based on by the long shoot trichoderma T6 bacterial strain wettable powder quality for 1g, each group distribution ratio are as follows:
In addition the present invention also provides a kind of long shoot trichoderma T6 bacterial strain wettable powder prevention and treatment alternaria leaf spot of apple and
Application in apple anthrax leaf blight.
The beneficial effects of the present invention are:
1. the new and effective long shoot trichoderma T6 bacterial strain wettable powder for researching and developing acquisition is alternaria leaf spot of apple and apple charcoal
The prevention and treatment of subcutaneous ulcer leaf blight provides new biological agent；
2. the long shoot trichoderma T6 bacterial strain wettable powder developed can effectively substitute pesticide residue caused by chemical pesticide,
Pollution environment, pathogen develop drug resistance and the drawbacks such as person poultry poisoning, develop tool to green and the direction that organises to Apple Industry
There is important meaning.
3. the long shoot trichoderma T6 bacterial strain wettable powder developed can effectively prevent apple spot defoliation and apple anthrax leaf is withered
The generation and harm of disease have significant economic and social benefit for the upgrading, volume increase, synergy of Apple Industry.
Detailed description of the invention
Fig. 1: fungistatic effect figure of the different agents to Alternaria mali；
Fig. 2: fungistatic effect figure of the different agents to apple anthrax leaf spoting bacteria；
Note: A: control；B: long shoot trichoderma T6 bacterial strain wettable powder.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the invention, rather than the whole invented.Based on the embodiments of the present invention, ordinary skill people
Member's every other embodiment obtained without making creative work, shall fall within the protection scope of the present invention.
Experimental material used in the following example and instrument include:
For trying biomaterial: Alternaria mali, apple anthrax leaf spoting bacteria and long shoot trichoderma T6 bacterial strain are by sweet
Respectful agriculture university's plant protection institute's plant aetiology laboratory saves and provides.
Insecticides adjuvant: wetting agent lauryl sodium sulfate is produced by Huntsman Corporation, dispersing agent sodium lignin sulfonate is by henry
The production of Si Mai company, sticker xanthan gum are by the production of Tianjin recovery fine chemistry industry research institute, conidia germination stimulus zinc sulfate
It is produced by the production of Shanghai Jinshan chemical plant, uv-protector carbon black by Zhengzhou Zheng Hao chemical products Co., Ltd, carrier talc
By the production of Tianjin recovery fine chemistry industry research institute
For test instrument: high-pressure steam sterilizing pan, shaking table, microscope, blood counting chamber, hand sprayer, airslide disintegrating mill
(Hua Lisuofei Science and Technology Ltd. of Shanghai East China University of Science and Technology), ultraviolet lamp tube (Shanghai Yi Hui instrument and meter Co., Ltd) and small
The instrument of type high velocity fog drying instrument (Shanghai Shi Yuan biological plant Co., Ltd), other undeclared sources is commercial product.
A kind of long shoot trichoderma T6 bacterial strain wettable powder agent prescription of embodiment 1
A kind of long shoot trichoderma T6 bacterial strain wettable powder by quality for 1g based on, each group distribution ratio are as follows:
A kind of quality testing of long shoot trichoderma T6 bacterial strain wettable powder in 2 embodiment 1 of embodiment
Under the component proportion, the spore suspension rate of long shoot trichoderma T6 bacterial strain wettable powder be (80.11 ± 0.89) %,
Total suspension is (76 ± 1.23) %, wetting time be (52 ± 0.54) s, pH be (6.24 ± 0.181), water content is (2.02
± 0.14) %, standard screen sieving rate are (97 ± 0.54) % and spore count living is 2.67 × 109A/g meets national wettable
The relevant criterion of pulvis and biological agent.
The bacteriostasis of one of 3 embodiment 1 of embodiment long shoot trichoderma T6 bacterial strain wettable powder agent prescription measures
Long shoot trichoderma T6 wettable powder prepared by embodiment 1 is added in PDA culture medium, gently shakes and is allowed to mix system
At plate, the plate of equivalent sterile water is added as control.Then, apple spot fallen leaves are picked them separately and apple anthrax leaf is withered
The pure culture biscuits involvng inoculation of germ is each to handle and compare equal 3 repetitions to plate center.In the incubator that temperature is (25 ± 1) DEG C
Culture, and 3d starts the bacterium using " crossing method " measurement apple spot fallen leaves and apple anthrax leaf spoting bacteria after culture
Diameter is fallen, calculates each processing bacteriostasis rate with following formula.
Bacteriostasis rate (%)=[(control colony diameter-processing colony diameter)/(control colony diameter -0.5)] × 100
Note: 0.5 is the diameter (cm) for accessing bacteria cake in formula.
Select alternaria leaf spot of apple and apple anthrax leaf blight to the control efficiency of long shoot trichoderma T6 wettable powder into
Row measurement.It is obtained by table 1, long shoot trichoderma T6 wettable powder is indoors to Alternaria mali and apple anthrax leaf blight
The bacteriostasis rate of bacterium is respectively 80.63% and 93.75%.Fungistatic effect is as shown in Figure 1, 2.
Fungistatic effect of 1 different agents of table to Alternaria mali and apple anthrax leaf spoting bacteria
The measurement of one of 4 embodiment 1 of embodiment long shoot trichoderma T6 bacterial strain wettable powder agent prescription control efficiency
By the healthy apple newly slightly blade cuttage acquired from test orchard in the triangular flask for filling sterile water, 8 every bottle,
And use the method for spray inoculation respectively by the spore suspension of the apple spot fallen leaves of culture and anthrax leaf spoting bacteria (concentration for
1×107A/mL) front and back sides for trying Apple Leaves are inoculated in, plastic jacket moisturizing (relative humidity about 90%) is placed in 25 DEG C
Climate box in cultivate.After Bacteria culturing 3d to be seeded, using spray-on process by the long shoot trichoderma T6 bacterial strain wettable powder of research and development
Agent (800 times of liquid) is uniformly sprayed on the leave dual sides of inoculation pathogen.Each processing and compare equal 3 repetitions in test, i.e., 3
Bottle totally 24 branches.Then, it after cultivating 7d, is sent out according to the generation grade scale of apple spot fallen leaves and anthrax leaf blight
State of an illness condition investigation statistics calculate control efficiency.
Control efficiency (%)=[(control disease index-processing disease index)/control disease index] × 100
Long shoot trichoderma T6 bacterial strain wettable powder is selected to imitate the prevention and treatment of alternaria leaf spot of apple and apple anthrax leaf blight
Fruit is measured.It is obtained by table 2, long shoot trichoderma T6 wettable powder prevents alternaria leaf spot of apple and apple anthrax leaf blight
Controlling effect is respectively 80.12% and 84.54%.
2 different agents of table are to Alternaria mali and apple anthrax leaf blight control efficiency
Claims (9)
1. a kind of long shoot trichoderma T6 bacterial strain wettable powder, which is characterized in that in percentage by weight, the wettable powder
Effective active composition includes 10% long shoot trichoderma T6 bacterial strain conidial powder.
2. a kind of long shoot trichoderma T6 bacterial strain wettable powder as described in claim 1, which is characterized in that percentage
It calculates, which further includes 0.6% wetting agent, 5% dispersing agent, 0.05% sticker, 0.1% spore germination promotion
Agent, 0.3% uv-protector, balance carriers talcum powder.
3. a kind of long shoot trichoderma T6 bacterial strain wettable powder as claimed in claim 2, which is characterized in that the wetting agent is
Lauryl sodium sulfate, concentration are 60 μ g/mL.
4. a kind of long shoot trichoderma T6 bacterial strain wettable powder as claimed in claim 2, which is characterized in that the dispersing agent is
Sodium lignin sulfonate.
5. a kind of long shoot trichoderma T6 bacterial strain wettable powder as claimed in claim 2, which is characterized in that the sticker is
Xanthan gum.
6. a kind of long shoot trichoderma T6 bacterial strain wettable powder as claimed in claim 2, which is characterized in that the spore germination
Promotor is zinc sulfate, and concentration is 10 μ g/mL.
7. a kind of long shoot trichoderma T6 bacterial strain wettable powder as claimed in claim 2, which is characterized in that the ultraviolet protection
Agent is carbon black.
8. a kind of long shoot trichoderma T6 bacterial strain wettable powder as described in claim 1, which is characterized in that by the long shoot wood
Mould T6 bacterial strain wettable powder quality is 1g meter, each group distribution ratio are as follows:
Surplus is carrier talc.
9. a kind of long shoot trichoderma T6 bacterial strain wettable powder described in claim 1 is in prevention and treatment alternaria leaf spot of apple and apple charcoal
Application in subcutaneous ulcer leaf blight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811100846.7A CN109221246A (en) | 2018-09-20 | 2018-09-20 | A kind of long shoot trichoderma T6 bacterial strain wettable powder and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811100846.7A CN109221246A (en) | 2018-09-20 | 2018-09-20 | A kind of long shoot trichoderma T6 bacterial strain wettable powder and its application |
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| Publication Number | Publication Date |
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| CN109221246A true CN109221246A (en) | 2019-01-18 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201811100846.7A Pending CN109221246A (en) | 2018-09-20 | 2018-09-20 | A kind of long shoot trichoderma T6 bacterial strain wettable powder and its application |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111642520A (en) * | 2020-07-16 | 2020-09-11 | 浙江省柑桔研究所 | Paecilomyces lilacinus wettable powder and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104059860A (en) * | 2014-07-04 | 2014-09-24 | 江苏丘陵地区镇江农业科学研究所 | Trichoderma koningiopsis TZ-11 bacterial strain and application thereof |
| MX2013014682A (en) * | 2013-11-29 | 2015-05-28 | Univ Autonoma De Baja California | Trichoderma longibrachiatum thica-4 strain acting as a fungicide and promoting the growth of plants of agricultural interest. |
| CN106993631A (en) * | 2017-05-12 | 2017-08-01 | 浙江省柑桔研究所 | A kind of knife spore Verticillium dahliae wettable powder and its application |
-
2018
- 2018-09-20 CN CN201811100846.7A patent/CN109221246A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX2013014682A (en) * | 2013-11-29 | 2015-05-28 | Univ Autonoma De Baja California | Trichoderma longibrachiatum thica-4 strain acting as a fungicide and promoting the growth of plants of agricultural interest. |
| CN104059860A (en) * | 2014-07-04 | 2014-09-24 | 江苏丘陵地区镇江农业科学研究所 | Trichoderma koningiopsis TZ-11 bacterial strain and application thereof |
| CN106993631A (en) * | 2017-05-12 | 2017-08-01 | 浙江省柑桔研究所 | A kind of knife spore Verticillium dahliae wettable powder and its application |
Non-Patent Citations (4)
| Title |
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| O.A.DREHVAL 等: "Screening of strains of soil micromycetes–antagonists of fungal and bacterial plant pathogens", 《BIOSYSTEMS DIVERSITY》 * |
| 张建华 等: "长枝木霉 T6 发酵液稳定性及粗提物抑菌活性", 《农药》 * |
| 张素敏 等: "《园林植物病害发生与防治》", 30 September 2014, 中国农业大学出版社，第1版 * |
| 赵政阳 等: "《中国果树科学与实践》", 30 June 2015, 陕西科学技术出版社，第1版 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111642520A (en) * | 2020-07-16 | 2020-09-11 | 浙江省柑桔研究所 | Paecilomyces lilacinus wettable powder and application thereof |
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Application publication date: 20190118 |
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