CN107991471A - A kind of water-biomembrane visible ray shines analogue experiment installation - Google Patents

A kind of water-biomembrane visible ray shines analogue experiment installation Download PDF

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Publication number
CN107991471A
CN107991471A CN201711190668.7A CN201711190668A CN107991471A CN 107991471 A CN107991471 A CN 107991471A CN 201711190668 A CN201711190668 A CN 201711190668A CN 107991471 A CN107991471 A CN 107991471A
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CN
China
Prior art keywords
water
pump line
experiment
water inlet
light source
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CN201711190668.7A
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Chinese (zh)
Inventor
李明
钟爽
薛洪海
吕晨
李晨阳
刘滨硕
吴磊
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Jilin Jianzhu University
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Jilin Jianzhu University
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Priority to CN201711190668.7A priority Critical patent/CN107991471A/en
Publication of CN107991471A publication Critical patent/CN107991471A/en
Pending legal-status Critical Current

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N2033/0078Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00 testing material properties on manufactured objects

Abstract

The present invention provides a kind of aquatic organism film visible ray and shines analogue experiment installation,It includes light source,Water bath,Temperature controller,Water inlet pump line,Water outlet pump line,Magnetic stirring apparatus,Illumination experiment beaker,Unglazed experiment beaker and biomembrane fixed frame,Light source is multiple,The surface for being arranged on water bath of light source side by side,And the irradiating angle of each light source can be adjusted in synchronism,At least one circulating pump generator is additionally provided with water bath,By setting multiple water inlet pump lines and water outlet pump line,Enable to the constant of each neighbouring temperature of experiment beaker,Reduce influence of the temperature difference of each experiment beaker to simulated experiment result,The angle adjusting mechanism of visible light source,It is capable of the irradiating angle of effective simulated visible light to influence of the surface coatings in natural to pollutant removal,Improve the function of analogue experiment installation simulation,Circulating pump,The constant of regional water temperature in water bath can effectively be improved,Further improve simulated experiment precision.

Description

A kind of water-biomembrane visible ray shines analogue experiment installation
Technical field
The present invention relates to a kind of experimental provision, is specially that a kind of water-biomembrane visible ray shines analogue experiment installation, belongs to raw Thing film visible analog experimental technique field.
Background technology
In natural water, biomembrane is by microbial cell by the waterflooding surface of solids(Bacterium, microalgae etc.)With it is abiotic Material(Iron and manganese oxides and organic matter etc.)The microbiologic population assembled and formed.Biomembrane in surface water body generally existing but Commonly overlooked natural biology membrane interface.In recent years, research finds that removal of the biomembrane to water pollutant is most important, and light According to being very important environmental impact factor.The ultraviolet light of solar radiation arrival earth surface is less than 5% in nature, with visible Based on light.In some aqueous bio illumination experiments, such as in research illumination to surface coatings in natural degraded anionic surface Activating agent(Neopelex)In the experiment of influence, light source has the water temperature of simulated experiment system trace quantity mineral salting liquid A degree of influence, and temperature is to trace quantity mineral salting liquid water body parameter(DO and pH), natural water biology algae it is new old Metabolism and the degraded of organic pollution have different degrees of influence, increase the difficulty of research work, can not also obtain accurate Data, ultimately caused the direct effect that can not investigate illumination.Although in this regard, one kind of developmental research before Water-biomembrane visible ray shines analogue experiment installation, but the water temperature of this experimental provision can not fully achieve thermostatic control, Water temperature near each experiment beaker still easily goes out difference, so as to cause the simulated experiment result of each beaker with certain poor It is different, influence simulated experiment effect, meanwhile, the irradiating angle of light can only vertical irradiation, irradiating angle can not adjust, it is impossible to effectively The irradiating angle of simulated visible light pollutant effects are removed to surface coatings in natural, therefore, it is necessary to real to this simulation Experiment device is further improved, and to improve the model accuracy of analogue experiment installation, and makes it have simulated visible light photograph The function that firing angle degree influences aqueous bio film.
Based on above technical problem, the present invention provides a kind of water-biomembrane visible ray to shine analogue experiment installation, it passes through Multiple water inlet pump lines and multiple water outlet pump lines are set, the constant of each neighbouring temperature of experiment beaker is enabled to, reduces each reality Influence of the temperature difference of beaker to simulated experiment result is tested, meanwhile, there is provided the angle adjusting mechanism of visible light source, Neng Gouyou Influence of the irradiating angle of the simulated visible light of effect to surface coatings in natural degradation of contaminant, improves the analogue experiment installation The function of simulation, in addition, being also provided with circulating pump, accelerates water body flow in sink, so as to effectively improve in water bath Regional water temperature it is constant, further increase simulated experiment precision.
The content of the invention
The object of the present invention is to provide a kind of structure and using simple, reasonable, precision height, a kind of water-life that performance is stablized Thing film visible ray shines analogue experiment installation.
To achieve the above object, the present invention adopts the following technical scheme that:A kind of water-biomembrane visible ray is filled according to simulated experiment Put, it includes light source, water bath, temperature controller, water inlet pump line, water outlet pump line, magnetic stirring apparatus, illumination experiment beaker, nothing Light tests beaker and biomembrane fixed frame, and the light source is multiple, is set between the water bath and the temperature controller Have into water pump line and water outlet pump line, water-flow circuit formed between temperature controller, water inlet pump line, water bath and water outlet pump line, Multiple illumination experiment beakers and unglazed experiment beaker, the illumination experiment beaker and unglazed reality are provided with the water bath Test in beaker and be provided with biomembrane fixed frame, the magnetic stirring apparatus is provided with below the water bath, it is characterised in that institute The surface for being arranged on the water bath of light source side by side is stated, and the irradiating angle of each light source can be adjusted in synchronism, institute State and at least one circulating pump is additionally provided with water bath.
Further, preferably, the water inlet pump line includes at least water inlet branch pump line one, water inlet branch pump line two and water inlet branch Pump line three, wherein, the water inlet end of the water inlet branch pump line one, water inlet branch pump line two and water inlet branch pump line three with the constant temperature control Instrument connection processed, the water outlet of the water inlet branch pump line one, water inlet branch pump line two and water inlet branch pump line three connect with the water bath Connect, and the water bath is cylinder, the water inlet branch pump line one, water inlet branch pump line two and the circumferentially array of branch pump line three of intaking The upper end of the water bath is arranged on, the water outlet pump line includes at least water outlet branch pump line one and water outlet branch pump line two, wherein, institute State water outlet branch pump line one and the water bath, the water outlet branch pump line one and water outlet branch pump are connected with the water inlet end of water outlet branch pump line two The water outlet of pipe two connects the temperature controller, and the water inlet end Circle-Array of the water outlet branch pump line one and water outlet branch pump line two Row are arranged on the lower end of the water bath.
Further, preferably, present invention additionally comprises rotating base, the illumination experiment beaker and unglazed experiment beaker are equal It is spindle rotationally arranged in using the respective rotating base in the water bath.
Further, preferably, the outer surface coating of the unglazed experiment beaker is provided with one layer of tinfoil, the unglazed reality Test on the rim of a cup of beaker and be provided with nontransparent cup lid.
Further, preferably, the light source is greenhouse rare earth light source.
Further, preferably, present invention additionally comprises fixed frame, connection hanging rod, adjusting cylinder and fixed holder of lampshade, its In, the upper end of the connection hanging rod is rotatably hingedly arranged on the fixed frame, and the lower end of the connection hanging rod is rotatably cut with scissors Connect and be arranged on the fixed holder of lampshade, lampshade is fixedly installed under the fixed holder of lampshade, each light source is arranged at In the respective lampshade, the middle and lower part of the connection hanging rod is also rotatably hingedly arranged on the piston rod for adjusting cylinder, institute The cylinder body for stating adjusting cylinder is rotatably hingedly arranged on the fixed frame, and the adjusting cylinder of each light source is by same control Device Synchronization Control.
The beneficial effects of the present invention are:
A kind of water-biomembrane visible ray provided by the invention shines analogue experiment installation, it is by setting multiple water inlet pump lines and more A water outlet pump line, enables to the constant of each neighbouring temperature of experiment beaker, reduces the temperature difference of each experiment beaker to mould The influence of draft experiment result, meanwhile, there is provided the angle adjusting mechanism of visible light source, it is capable of the irradiation of effective simulated visible light Influence of the angle to surface coatings in natural degradation of contaminant, improves the function of analogue experiment installation simulation, in addition, also setting Circulating pump has been put, the constant of regional water temperature in water bath can have effectively been improved, further increase simulated experiment precision.
Brief description of the drawings
Fig. 1 is a kind of structure diagram of water-biomembrane visible ray according to analogue experiment installation of the present invention;
Wherein, 1, light source, 2, water bath, 3, temperature controller, 4, water inlet pump line, 5, water outlet pump line, 6, magnetic stirring apparatus, 7, light According to the facts test beaker, 8, unglazed experiment beaker, 9, biomembrane fixed frame, 10, water inlet branch pump line one, 11, water inlet branch pump line two, 12, Intake branch pump line three, 13, water outlet branch pump line one, 14, water outlet branch pump line two, 15, nontransparent cup lid, 16, fixed frame, 17, connection Hanging rod, 18, adjust cylinder, 19, fixed holder of lampshade, 20, circulating pump, 21, rotating base.
Embodiment
Come to carry out the present invention detailed description below in conjunction with attached drawing.It should be appreciated, however, that attached drawing has been provided only more Understand the present invention well, they should not be interpreted as limitation of the present invention.
As shown in Figure 1, the present invention, which provides a kind of water-biomembrane visible ray, shines analogue experiment installation, it includes light source 1, water Bath 2, temperature controller 3, water inlet pump line 4, water outlet pump line 5, magnetic stirring apparatus 6, illumination experiment beaker 7, unglazed experiment beaker 8 With biomembrane fixed frame 9, the light source 1 is multiple, is provided between the water bath 2 and the temperature controller 3 into water Pump line 4 and water outlet pump line 5, water-flow circuit is formed between temperature controller 3, water inlet pump line 4, water bath 2 and water outlet pump line 5, Multiple illumination experiment beakers 7 and unglazed experiment beaker 8, the illumination experiment beaker 7 and nothing are provided with the water bath 2 Biomembrane fixed frame 9 is provided with light experiment beaker 8, the lower section of the water bath 2 is provided with the magnetic stirring apparatus 6, it is special Sign is, the surface for being arranged on the water bath 2 of the light source 1 side by side, and the irradiating angle of each light source 1 can It is adjusted in synchronism, at least one circulating pump 20 is additionally provided with the water bath 2.
In the present embodiment, the water inlet pump line 4 includes at least water inlet branch pump line 1, water inlet branch pump line 2 11 and water inlet Branch pump line 3 12, wherein, the water inlet end of the water inlet branch pump line 1, water inlet branch pump line 2 11 and water inlet branch pump line 3 12 with The temperature controller connection, the water outlet of the water inlet branch pump line 1, water inlet branch pump line 2 11 and water inlet branch pump line 3 12 It is connected with the water bath 2, and the water bath 2 is cylinder, the water inlet branch pump line 1,2 11 and of water inlet branch pump line Circumferentially array is arranged on the upper end of the water bath to water inlet branch pump line 3 12, and the water outlet pump line 5 includes at least water outlet branch pump Pipe 1 and water outlet branch pump line 2 14, wherein, the water outlet branch pump line 1 connects institute with the water inlet end of water outlet branch pump line 2 14 Water bath 2 is stated, the water outlet branch pump line 1 connects the temperature controller with the water outlet of water outlet branch pump line 2 14, and described The water inlet end circumference array of water outlet branch pump line 1 and water outlet branch pump line 2 14 is arranged on the lower end of the water bath 2.
In the present embodiment, present invention additionally comprises rotating base 21, the illumination experiment beaker and unglazed experiment beaker are equal It is spindle rotationally arranged in using the respective rotating base 21 in the water bath 2.The outer surface of the unglazed experiment beaker Coating is provided with one layer of tinfoil, and nontransparent cup lid is provided with the rim of a cup of the unglazed experiment beaker.The light source is dilute for greenhouse Native light source.
In addition, present invention additionally comprises fixed frame 16, connection hanging rod 17, cylinder 18 and fixed holder of lampshade 19 are adjusted, wherein, institute The upper end for stating connection hanging rod 17 is rotatably hingedly arranged on the fixed frame 16, and the lower end of the connection hanging rod 17 is rotatably cut with scissors Connect and be arranged on the fixed holder of lampshade 19, lampshade is fixedly installed under the fixed holder of lampshade 19, each light source is all provided with Put in the respective lampshade, the middle and lower part of the connection hanging rod 17 is also rotatably hingedly arranged on the piston for adjusting cylinder 18 On bar, the cylinder body for adjusting cylinder 18 is rotatably hingedly arranged on the fixed frame 16, the adjusting gas of each light source Cylinder is by same controller Synchronization Control.
Embodiment:
Application of the product in the experiment that new micropollutants influence is degraded surface coatings in natural in research illumination.
During using the present invention, each beaker 1 is injected into trace quantity mineral salting liquid first(MMS), component in MMS solution and Concentration, see the table below,
Trace quantity mineral salting liquid(MMS)Its component and concentration
Component Concentration(μmol/L)
CaCl2 200
MgSO4 140
(NH4)2SO4 910
KNO3 150
NaHCO3 10
Secondly the biomembrane needed for testing(Biomembrane fixed frame)Parallelization is on biomembrane fixed frame, adjacent biomembrane About 8 mm are spaced between fixed frame, then immerse biomembrane fixed frame and biomembrane in beaker MMS solution together.Biomembrane is consolidated It is about 70 ° to determine the angle between frame and horizontal plane, so can ensure that every biomembrane is consolidated during simulated experiment as far as possible The light conditions for determining frame are consistent.
Wherein, the cultural method of surface coatings in natural is as follows:Natural Water is used under the conditions of natural lighting in laboratory Body is cultivated.Use biomembrane fixed frame(48 mm × 75 mm × 1 mm)As biofilm medium, by biomembrane Fixed frame is fixed on polystyrene frame(The patent No.:ZL01202517.8)After be put into aquarium(60 cm × 30 cm × 50 cm), about at the 30cm of underwater.Before use, biomembrane fixed frame and polystyrene frame are first scrubbed two with cleaning agent Time, then cleaned twice with ultra-pure water.The hydromining that uses of culture surface coatings in natural is from Natural Waters such as river, lake and wetlands Body.MMS solution is added in surface coatings in natural cultivating system, during biomembrane culture pH and DO about 7.3 ~ 8.9, 8.0 ~ 14.5 mg/L, every 3 ~ 5 days supply culture water bodys.Biofilm medium matter in order to prevent(Biomembrane fixed frame)Come off, Biological film culture device should try one's best in underwater keeps horizontality.In order to make to adhere on each biomembrane fixed frame to the full extent Bacterium algae and inorganic component be evenly distributed, reduce the difference of biomembrane on each biomembrane fixed frame, thus will as far as possible make whole Ambient environmental conditions residing for biomembrane fixed frame are identical.During biomembrane culture, about at interval of 2 ~ 3 days according to certain sky Between sequentially exchange dimensional orientation of the polypropylene shelf in cultivating system.Culture substrate surface presents obvious equal after about two weeks Even breen, this explanation biomembrane have formed on biomembrane fixed frame surface, can be used to test.In this research, with life The piece number of thing film represents biofilm biomass.
In terms of illumination, this research uses hothouse plants rare earth light source as experimental light sources, for simulated solar irradiation.Test light Source spectrum(Apart from about 1 m of light source)Approached with the spatial distribution and intensity of sunlight, spectrum is concentrated mainly on visible region, purple Exterior domain can be ignored.During simulated experiment, light source is located above simulated experiment system at about 1 m, and light irradiates from top to bottom. In order to ensure for intensity of illumination than more uniform, the present invention uses three rare earth light sources, each beaker of simulated experiment system in Experimental Area In biomembrane obtained by effectively intensity of illumination be about 40000 ± 3000 Lux.For no light experimental group, by beaker Wall is wrapped up with tinfoil and is capped at the same time at top, and the intensity of illumination in beaker is less than 5 Lux at this time, can use controller to adjusting Cylinder is controlled, and realizes that the angle synchronizing moving of light source is adjusted.
Temperature control water circulation system is opened in experiment so that water bath is connected by pumping plant keeps water to circulate shape State, accelerates water temperature distributing equilibrium in groove;The rotor of magnetic stirring apparatus control continuously stirs, and can make in simulated experiment beaker Material mixing it is uniform, the temperature in all beakers of during the experiment is controlled in 22.5 ± 1 oC.
This experimental provision reduces the influence that temperature change is brought to simulated experiment, has preferably investigated illumination to Natural Water The influence of the new micropollutants of body biology membrane degradation.
A kind of water-biomembrane visible ray provided by the invention shines analogue experiment installation, it is by setting multiple water inlet pump lines With multiple water outlet pump lines, each constant, temperature difference of each experiment beaker of reduction for testing temperature near beaker is enabled to Influence to simulated experiment result, meanwhile, being capable of effective simulated visible light there is provided the angle adjusting mechanism of visible light source Influence of the irradiating angle to the new micropollutants of surface coatings in natural degraded, improves the work(of analogue experiment installation simulation Can, in addition, be also provided with circulating pump, the constant of regional water temperature in water bath can be effectively improved, is further increased Simulated experiment precision.
Embodiment of above is merely to illustrate the present invention, and not limitation of the present invention, in relation to the common of technical field Technical staff, without departing from the spirit and scope of the present invention, can also make a variety of changes and modification, therefore all Equivalent technical solution falls within scope of the invention, and scope of patent protection of the invention should be defined by the claims.

Claims (6)

1. a kind of water-biomembrane visible ray shines analogue experiment installation, it is characterised in that it includes light source, water bath, thermostatic control Instrument, water inlet pump line, water outlet pump line, magnetic stirring apparatus, illumination experiment beaker, unglazed experiment beaker and biomembrane fixed frame, it is described Light source to be multiple, be provided between the water bath and the temperature controller into water pump line and water outlet pump line, thermostatic control Water-flow circuit is formed between instrument, water inlet pump line, water bath and water outlet pump line, multiple illumination are provided with the water bath Beaker and unglazed experiment beaker are tested, biomembrane fixed frame, institute are provided with the illumination experiment beaker and unglazed experiment beaker State and the magnetic stirring apparatus be provided with below water bath, it is characterised in that the light source side by side be arranged on the water bath Surface, and the irradiating angle of each light source can be adjusted in synchronism, and at least one follow is additionally provided with the water bath Ring pumps.
2. a kind of water-biomembrane visible ray according to claim 1 shines analogue experiment installation, it is characterised in that it is described into Water pump pipe includes at least water inlet branch pump line one, water inlet branch pump line two and water inlet branch pump line three, wherein, the water inlet branch pump line one, The water inlet end of water inlet branch pump line two and water inlet branch pump line three is connected with the temperature controller, the branch pump line one of intaking, into The water outlet of water branch pump line two and water inlet branch pump line three is connected with the water bath, and the water bath is cylinder, described Circumferentially array is arranged on the upper end of the water bath for water inlet branch pump line one, water inlet branch pump line two and water inlet branch pump line three, described Water outlet pump line includes at least water outlet branch pump line one and water outlet branch pump line two, wherein, the water outlet branch pump line one and water outlet branch pump line Two water inlet end connects the water bath, and the water outlet branch pump line one connects the constant temperature control with the water outlet of water outlet branch pump line two Instrument processed, and the water inlet end circumference array of the water outlet branch pump line one and water outlet branch pump line two is arranged on the lower end of the water bath.
3. a kind of water-biomembrane visible ray according to claim 1 shines analogue experiment installation, it is characterised in that further includes Rotating base, the illumination experiment beaker and unglazed experiment beaker are spindle rotationally arranged in using the respective rotating base In the water bath.
4. a kind of water-biomembrane visible ray according to claim 1 shines analogue experiment installation, it is characterised in that the nothing The outer surface coating of light experiment beaker is provided with one layer of tinfoil, and nontransparent cup is provided with the rim of a cup of the unglazed experiment beaker Lid.
5. a kind of water-biomembrane visible ray according to claim 1-4 any one shines analogue experiment installation, its feature exists In the light source is greenhouse rare earth light source.
6. a kind of water-biomembrane visible ray according to claim 1-4 any one shines analogue experiment installation, its feature exists In, further include fixed frame, connection hanging rod, adjust cylinder and fixed holder of lampshade, wherein, the upper end of the connection hanging rod is rotatably cut with scissors Connect and be arranged on the fixed frame, the lower end of the connection hanging rod is rotatably hingedly arranged on the fixed holder of lampshade, described Lampshade is fixedly installed under fixed holder of lampshade, each light source is arranged in the respective lampshade, the connection hanging rod Middle and lower part also rotatably hingedly be arranged on adjust cylinder piston rod on, it is described adjust cylinder cylinder body rotatably hingedly set On the fixed frame, the adjusting cylinder of each light source is by same controller Synchronization Control.
CN201711190668.7A 2017-11-24 2017-11-24 A kind of water-biomembrane visible ray shines analogue experiment installation Pending CN107991471A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115102614A (en) * 2022-05-23 2022-09-23 中航光电科技股份有限公司 Full-duplex underwater visible light MIMO communication system with decontamination function

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Publication number Priority date Publication date Assignee Title
CN115102614A (en) * 2022-05-23 2022-09-23 中航光电科技股份有限公司 Full-duplex underwater visible light MIMO communication system with decontamination function
CN115102614B (en) * 2022-05-23 2023-07-21 中航光电科技股份有限公司 Full duplex underwater visible light MIMO communication system with decontamination function

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