CN107991443A - Application of the rice quality index in differentiating ratooning rice first rice crop rice and regenerating season rice - Google Patents
Application of the rice quality index in differentiating ratooning rice first rice crop rice and regenerating season rice Download PDFInfo
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- CN107991443A CN107991443A CN201711222444.XA CN201711222444A CN107991443A CN 107991443 A CN107991443 A CN 107991443A CN 201711222444 A CN201711222444 A CN 201711222444A CN 107991443 A CN107991443 A CN 107991443A
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- rice
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- regeneration
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/02—Food
- G01N33/10—Starch-containing substances, e.g. dough
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
Abstract
The invention discloses application of the rice quality index in differentiating ratooning rice first rice crop rice and regenerating season rice, ratooning rice first rice crop rice is detected by current conventional means and regenerates the index of quality in season rice, to judge first rice crop rice and regeneration season rice, regenerate chalky grain rate in season rice seed, chalkiness degree, amylose content and caustic SCC possess significant difference with the desired value in first rice crop seed, and chalky grain rate, the desired value of chalkiness degree is substantially lower than first rice crop rice, and the desired value of amylose content and caustic SCC is substantially higher than first rice crop rice.The present invention can accurately differentiate ratooning rice head season and regeneration season rice, and assay method is easy, and cost of determination is relatively low.Technical measures of the present invention can be widely applied to different rice and make ecotope discriminating ratooning rice head season and regeneration season rice.
Description
Technical field
The invention belongs to crops field, is more particularly to rice quality index and is differentiating ratooning rice first rice crop rice and regeneration
Application in season rice.
Background technology
Ratooning rice is by corresponding field management measure, makes the Dormant Buds after gathering in a season on rice stub, ears, opens
Spend Rice Cropping pattern that is solid and harvesting again.Ratooning rice has input and output rate height, efficiency height, saving of labor, province kind, section
The features such as water.Be south China rice region plantation one season rice heat have a surplus and plant area and the double cropping of rice of double cropping of rice heat deficiency
Area only plant one season semilate rice rice field raise the multiple-cropping index, increase one of effective measures of yield per unit area and income.Cause
This, development ratooning rice is to adapting to current agricultural structural adjustment, improving grain yield, ensure grain security and tool of increasing farmers' income
It is significant.Recently as rice varieties improvement and the raising of cultivation management measure, ratooning rice head season and regeneration season yield
Have and be greatly improved very much.In addition, substantial amounts of research shows, regeneration season rice quality is higher than head season, can generally reach
National standard superior rice two level or level-one.Based on the good economic benefit of high-quality regeneration season rice, many agricultural cooperative societys and peasant
All actively expand ratooning rice plantation.Such as association of Chun Lu proportion of crop planting Specialty Co-operative Organization of Honghu in Hubei province ratooning rice growing surface
Product reaches 80,000 mu, while also applies for " Honghu ratooning rice " geographical sign to the Ministry of Agriculture.In many ratooning rice producing regions, Ji Shui is regenerated
Rice purchasing price is higher than head season.
With the higher economic benefit that regeneration season rice is brought, pretend to be the problem of regenerating season rice gradual with first rice crop rice
It is prominent.Regeneration season rice is for head season, so differentiating that first rice crop and regeneration season rice are just particularly important.Cause
This, identification ratooning rice first rice crop rice and regeneration season rice, ensure that the authenticity of regeneration season rice is positive for protection peasant's production
Property, the benefit of planting foods for ensureing peasant and the just rights and interests for safeguarding consumer have important meaning.But there is presently no on such as
The method what differentiates ratooning rice head season and regeneration season rice.Therefore, research purpose of the present invention differentiates ratooning rice head to establish one kind
Season and the method for regeneration season rice, to safeguard peasant and consumers' rights and interests, Maintenance Market order, promotes ratooning rice sustainable development
Technical support is provided with guarantee national food security.
The content of the invention
In view of the above-mentioned problems, the present invention provides rice quality index to differentiate ratooning rice first rice crop rice and regeneration season rice
In application, it has been found that in first rice crop rice and regeneration season rice, there were significant differences for some index of quality of rice, because
This, can determine ratooning rice first rice crop rice and regeneration season rice by detecting these index of quality.
In order to achieve the above object, the present invention takes following technical measures：
Application of the rice quality index in differentiating ratooning rice first rice crop rice and regenerating season rice, passes through current conventional hand
The index of quality in section detection ratooning rice first rice crop rice and regeneration season rice, it is described to judge first rice crop rice and regeneration season rice
The index of rice quality include：Chalky grain rate, chalkiness degree, amylose content and caustic SCC.
Above-described application, it is preferred that the first rice crop rice of ratooning rice and the criterion of regeneration season rice are：Regenerate season
Chalky grain rate in rice seed, chalkiness degree, amylose content and caustic SCC possess conspicuousness with the desired value in first rice crop seed
Difference, and chalky grain rate, the desired value of chalkiness degree is substantially lower than first rice crop rice, and the desired value of amylose content and caustic SCC is bright
It is aobvious higher than first rice crop rice.
Above-described application, it is preferred that ratooning rice first rice crop rice and the criterion of regeneration season rice are：Regenerate season rice
Chalky grain rate and chalkiness degree ratio head season low 40-85% and 55-95% in rice, and regenerate season rice grain amylose content and caustic SCC
Higher 5-60% than head season respectively and 5-165%.
Above-described application, it is preferred that the rice varieties are：It is Huang Huazhan, day two excellent 616, new two excellent 223, rich
Two excellent fragrant No. 1, two excellent 6326, standard two excellent 199, deep two excellent 5814, fragrant No. 1 middle, Feng You 9918 or Feng You king.
Compared with prior art, the present invention has the following advantages：
Differentiate ratooning rice first rice crop rice and regenerate the basis that season rice is to ensure that regeneration season rice authenticity, be to safeguard peasant
With the important means of consumers' rights and interests.The present invention can accurately differentiate ratooning rice head season and regeneration season rice, and assay method
Simplicity, cost of determination are relatively low.Technical measures of the present invention can be widely applied to different rice and make ecotope discriminating regeneration
Rice head season and regeneration season rice.
Embodiment
Technical solution of the present invention, is the conventional scheme of this area if not otherwise specified；The reagent or material,
If not otherwise specified, commercial channel is derived from.
Embodiment 1：
The otherness index of quality of ratooning rice first rice crop rice and regeneration season rice determines：
1. test place：This research selection is regional positioned at Huangzhou, Qichun, Tuanfeng County, Wuxue City and the Xishui of Southeastern Hubei etc..These
The plantation of regional ratooning rice is with a long history, and ratooning rice cultivated area is big.
2. experimental cultivar：Kind is pushed away in the local ratooning rice production of each area selection.
Huangzhou：Huang Huazhan, day two excellent 616, new two excellent 223 and rich two excellent fragrant No. 1；
Qichun：Rich two excellent fragrant No. 1 and two excellent 6326；
Tuanfeng County：Standard two excellent 199；
Wuxue City：Middle fragrant No. 1, two excellent 6326 and Huang Huazhan；
Xishui：Deep two excellent 5814.
3. field selects：In same area, for each kind, the three pieces of peasant for planting kind fields are randomly selected
Block.
4. field management：Each department ratooning rice head season is with regeneration season field management by field householder according to local ratooning rice
Cultivation management mode carries out.
A 5. season sampling：When ratooning rice head season rice is ripe, 3 500 grams of weights are taken at random in the field of selection
Seed samples.
6. regenerate season sampling：When ratooning rice regeneration season rice is ripe, 3 weights 500 are taken at random in the field of selection
Gram seed samples.
7. the measure of chalky grain rate and chalkiness degree：
1) 200 are taken at random from whole grain polished rice, is placed on black substrate (such as black glazed paper) range estimation has chalk white
The grain of rice, calculates it and accounts for the percentage of sum, i.e. chalky grain rate.
2) taken at random from sample with the white whole grain polished rice 10 of chalk (less than 10 persons have number to take by real), the grain of rice is put down
It is placed on computation paper and faces observation, the percentage of whole grain of rice projected area is accounted for by grain range estimation chalk fine flour product, obtains its average value,
As Chalkiness size.
3) chalkiness degree is calculated according to the chalky grain rate of each sample, Chalkiness size, i.e., chalkiness degree=chalky grain rate × chalk is white
Size.
8. the measure of amylose content：
The measure of sample：
1) head milled rice sample 2-3g to be measured is weighed, rice flour is worn into flour mill；Again with the sample sifter in 0.25mm apertures by rice
Under dressing sieve.
2) the rice flour 0.1000g under sieve is weighed, is placed in 100ml volumetric flasks, adds 95% ethanol 1.0ml, jog capacity
Bottle, makes sample moistening scattered.
3) NaOH solution of 9.0ml 1.0mol/L is added, lye is slowly flowed down along bottleneck wall, rotational volume bottle, makes
Lye rinses the sample attached in bottle wall.
4) volumetric flask is placed in boiling water after boiling 10min and taken out, after being cooled to room temperature plus distilled water is settled to 100ml.
5) 5.0ml sample solutions are drawn, adds and has filled in the 100ml volumetric flasks of half bottle of distilled water, then to this volumetric flask
The acetic acid solution 1.0ml of middle addition 1.0mol/L, makes sample acidifying (interference for reducing amylopectin).
6) 1.5ml iodine reagents are added, are fully shaken up, 100ml is settled to distilled water, stands 20min.
7) sample is replaced with the NaOH solution of the 0.09mol/L of 5.0ml, prepares blank solution.
8) zero point is adjusted at spectrophotometer wavelength 620nm with blank solution, and measures the absorbance of colored samples.
The drafting of standard curve and the calculating of sample amylose content：
1) 4 standard samples that the similarity condition known amylose content of lower more than 3 days is stored in sample to be tested are weighed
(its amylose content is left 1%, 10%, 20%, 30% respectively in advance through GB/T15683-2008 method Accurate Determinings for product
It is right) each 0.1000g, with above-mentioned improvement short cut technique, measure is carried out at the same time with sample to be tested.
2) abscissa is made with absorbance, amylose content makees ordinate, draws standard curve；Or according to 4 standards
The amylose content (y) of sample and corresponding absorbance (x) establish the regression equation (y=a+bx) of standard curve.
3) amylose content in polished rice sample can directly be read with the absorbance of sample from standard curve, also may be used
The absorbance of sample is substituted into regression equation and is calculated.
9. the measure of caustic SCC：
1) the complete head milled rice of every part of material selection 6, is put into the culture dish of a diameter of 60mm, is repeated 2 times.
2) the KOH solution 5.0ml of 1.7mol/L is added into ware with pipette, and the grain of rice is arranged uniformly, makes each grain of rice
Between leave equal gap.
3) ware lid is covered, culture dish is put into insulating box (30 DEG C), when standing 23 is small.
4) carefully culture dish is taken out from insulating box and (sample must not be rocked), be placed on the table top for having padded black cloth
On, estimate the appearance of endosperm and digest diffusion.
5) the average caustic SCC (level) of each 6 grain of rices of sample is calculated by following equation：Caustic SCC (level)=each grain of rice alkali
Summation/6 for value (level) that disappear.
6) the double repeated experiment error of every part of sample should be no more than 0.5 grade, seek the average value repeated twice, as measure
As a result.
Caustic SCC grade scale (GB/T17891-1999)
Analysis of experimental results
This research determines rice chalky grain rate, chalkiness degree, amylose content and caustic SCC.Can from 1 result of table
Go out, ratooning rice head season and regeneration season chalky grain rate, chalkiness degree, the difference of amylose content and caustic SCC is in different location and product
Showed between kind consistent.Regeneration season rice chalky grain rate and chalkiness degree are substantially less than head season rice.With head aspect ratio, regeneration season rice
The reduction amplitude of rice chalky grain rate is 46-82%, and the reduction amplitude of chalkiness degree is 61-86%.Regenerate the amylose of season rice
Content and caustic SCC are all significantly higher than a season in all places, and regeneration season rice grain amylose content and caustic SCC averagely compare respectively
Head season high 6-56% and 9-162%.Therefore, applicant thinks to measure and compares ratooning rice head season and the regeneration season white grain of rice chalk
Rate, chalkiness degree, amylose content and caustic SCC can identify difference head season and regeneration season rice.
The comparison of 1. ratooning rice of table head season seed and regeneration season seed constituent content
The same kind in same place in same column, marks different alphabetical values notable in P=0.05 level differences.
Embodiment 2：
Application of the rice quality index in differentiating ratooning rice first rice crop rice and regenerating season rice：
1. test place：This research is in addition positioned at the Honghu of Jianghan Plain, Jiangling, Jianli, Jing Zhou, Shashi and Zhijiang
Deng 5 areas field is determined according to the method introduced in embodiment 1.
2. experimental cultivar：Kind is pushed away in the local ratooning rice production of each area selection.
Honghu：Rich two excellent fragrant No. 1；
Jiangling：Rich two excellent fragrant No. 1；
Jianli：Feng You 9918 and Feng You king；
Jing Zhou：It two excellent 616；
Shashi：Rich two excellent fragrant No. 1.
3. field management：Each department ratooning rice head season is with regeneration season field management by field householder according to local ratooning rice
Cultivation management mode carries out.
4. it is measured by sampling：Method according to being introduced in embodiment 1 samples and measures a season and regenerate season rice chalkiness grain rate,
Chalkiness degree, amylose content and caustic SCC.
Interpretation of result
Table 2 is shown, regional in Honghu, Jiangling, Jianli, Jing Zhou and Shashi etc., ratooning rice regeneration season rice chalkiness grain rate and
Chalkiness degree is substantially less than head season, and regeneration season amylose content and caustic SCC are significantly higher than a season.Regenerate season rice chalkiness grain rate
With chalkiness degree is lower 44-85% than head season respectively and 56-91%, regeneration season rice grain amylose content and caustic SCC are respectively than head season
High 30-57% and 10-128%.This result and 1 result of embodiment are completely the same, therefore confirm and measure and compare ratooning rice head
Season and regeneration season rice chalky grain rate, chalkiness degree, amylose content and caustic SCC can identify difference head season and regeneration season rice
Rice.
Above testing result is consistent completely with actual result, therefore can be by measuring rice chalkiness grain rate and chalkiness degree, directly
Chain content of starch and caustic SCC, identification differentiation is carried out to ratooning rice first rice crop and regeneration season rice.
The comparison of 2. ratooning rice of table head season and regeneration season seed boron, arsenic and molybdenum concentration
The same kind in same place in same column, marks different alphabetical values notable in P=0.05 level differences.
By to other areas, regenerating the chalky grain rate in season rice and first rice crop, chalkiness degree, amylose content and alkali disappear
Value is detected, chalky grain rate and chalkiness degree ratio head season low 40-85% and 55-95% in regeneration season rice, and regenerates season rice
Amylose content and caustic SCC is higher 5-60% than head season respectively and 5-165%..
Claims (4)
1. application of the rice quality index in differentiating ratooning rice first rice crop rice and regenerating season rice, the finger of the rice quality
Mark includes：Chalky grain rate, chalkiness degree, amylose content and caustic SCC.
2. application according to claim 1, the criterion of the first rice crop rice and regeneration season rice of the ratooning rice are：
Chalky grain rate, chalkiness degree, amylose content and caustic SCC possess with the desired value in first rice crop seed in regeneration season rice seed
Significant difference, and chalky grain rate, the desired value of chalkiness degree is substantially lower than first rice crop rice, the finger of amylose content and caustic SCC
Scale value is substantially higher than first rice crop rice.
3. application according to claim 1, the criterion of the ratooning rice first rice crop rice and regeneration season rice are：Again
Chalky grain rate and chalkiness degree ratio head season low 40-85% and 55-95% in raw season rice, and regenerate season rice grain amylose content and alkali
The value that disappears is higher 5-60% than head season respectively and 5-165%.
4. application according to claim 1, the rice varieties are：It is Huang Huazhan, day two excellent 616, new two excellent 223, rich
Two excellent fragrant No. 1, two excellent 6326, standard two excellent 199, deep two excellent 5814, fragrant No. 1 middle, Feng You 9918 or Feng You king.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111272959A (en) * | 2020-03-04 | 2020-06-12 | 中国水稻研究所 | Method for judging quality stability of multi-year/multi-point rice variety |
| CN111289706A (en) * | 2019-08-13 | 2020-06-16 | 中国水稻研究所 | Physical and chemical screening method of japonica rice varieties suitable for making rice balls |
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| CN101743871A (en) * | 2009-12-21 | 2010-06-23 | 江安县农业技术推广站 | Compartment-opening, strengthening, no-tillage, water-saving and integrating technique of rice |
| CN105104174A (en) * | 2015-09-18 | 2015-12-02 | 江苏丘陵地区镇江农业科学研究所 | Cultivation method for high-yield japonica rice variety with good taste |
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2017
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101743871A (en) * | 2009-12-21 | 2010-06-23 | 江安县农业技术推广站 | Compartment-opening, strengthening, no-tillage, water-saving and integrating technique of rice |
| CN105067784B (en) * | 2015-07-22 | 2016-08-24 | 中国水稻研究所 | Utilize the physicochemical character index of rice quality to the method judging rice eating-quality |
| CN105104174A (en) * | 2015-09-18 | 2015-12-02 | 江苏丘陵地区镇江农业科学研究所 | Cultivation method for high-yield japonica rice variety with good taste |
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| 刘国华 等: "中稻头季稻与再生稻的品质比较研究", 《杂交水稻》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111289706A (en) * | 2019-08-13 | 2020-06-16 | 中国水稻研究所 | Physical and chemical screening method of japonica rice varieties suitable for making rice balls |
| CN111289706B (en) * | 2019-08-13 | 2021-05-18 | 中国水稻研究所 | Physical and chemical screening method of japonica rice varieties suitable for making rice balls |
| CN111272959A (en) * | 2020-03-04 | 2020-06-12 | 中国水稻研究所 | Method for judging quality stability of multi-year/multi-point rice variety |
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Application publication date: 20180504 |