CN104542025B - Early screening method for good varieties of bundle plant type nobile-type dendrobium - Google Patents
Early screening method for good varieties of bundle plant type nobile-type dendrobium Download PDFInfo
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- CN104542025B CN104542025B CN201410711122.1A CN201410711122A CN104542025B CN 104542025 B CN104542025 B CN 104542025B CN 201410711122 A CN201410711122 A CN 201410711122A CN 104542025 B CN104542025 B CN 104542025B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
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- Biodiversity & Conservation Biology (AREA)
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Abstract
The invention discloses an early screening method for good varieties of bundle plant type nobile-type dendrobium. The method comprises the following steps: a, determining the number of leaves and the number of segments of one-year-old seedlings in a leaf termination period; b, respectively substituting the mean values of the number of leaves and the number of segments into corresponding linear equations y1=2.061x1(the number of leaves)-8.060, and y2=1.534x2 (the number of segments)-7.654; calculating the comprehensive score of the quality of nobile-type dendrobium flowering; c, predicting the nobile-type dendrobium flowering quality according to the comprehensive score of the quality of nobile-type dendrobium products and a variety grade dividing reference standard. According to the early screening method for good varieties of the bundle plant type nobile-type dendrobium disclosed by the invention, the variety screening efficiency is improved; the screening cycle is shortened; the production cost is reduced.
Description
Technical field
The present invention relates to plant variety screening technique, particularly to the side of a kind of clump of plant type spring Herba Dendrobii kind early screening
Method.
Background technology
Spring Herba Dendrobii is favored by global consumer because deep the features such as flower pattern pattern is abundant, the florescence is long, is when today, English, U.S. etc.
State popular top grade potted flower.
The plant type of dendrobium nobile potted flower can be divided into individual plant type and clump plant type, and the former has obvious major branch, and branch is few, general only main
Branch is bloomed, and joint number of blooming is more, and plant is tall and big, sturdy；The latter's major branch is inconspicuous, and branch is more, Flowering Branches quantity >=2, always
Branch quantity >=4, plant is relatively short, pseudobulb is thinner.
Because spring Herba Dendrobii is perennial plant, the trophophase is longer, and Plant technique just can be bloomed for 3 years.Using traditional large area
The method planted experimentally, the screening cycle is long, and production cost is high.Therefore, the research carrying out spring Herba Dendrobii improved seeds early screening very must
Will.
CN201310397973.9 discloses a kind of spring Herba Dendrobii improved seeds early screening method, according to institute in embodiment 1
State, it becomes flower quality evaluation more applicable for individual plant type spring Herba Dendrobii kind, and be not particularly suited for that branch is more, Flowering Branches quantity
>=2 clump plant type spring Herba Dendrobii kind.Because measuring into during colored index it is impossible to clearly measured which Flowering Branches.And simply clump
Plant type is processed as the combination plantation of individual plant type, and testing index is carried out simple superposition it is impossible to filter out improved seeds.In addition, shadow
Ring the one-tenth flower index of clump plant type Flowering quality 5 described in the embodiment 1 to bloom in addition to index, flower thorax ratio, always flower amount, Flowering Branches
And always branch all becomes colored quality closely related with it.Therefore, need to set up clump plant type becomes flower quality evaluation system and its improved seeds early
Phase screening technique.
Content of the invention
In order to solve the above problems, the method that the present invention provides a kind of clump of plant type spring Herba Dendrobii improved seeds early screening, bag
Include following steps：
A. measure annual seedling morphology index：Measure its number of sheets and joint number in spring Herba Dendrobii seedling leaf phase of stopping；
B. calculate spring Herba Dendrobii and become flower quality comprehensive score：Randomly draw the annual seedling being in the leaf phase of stopping, measure leaf
Number and joint number value, take average to substitute into corresponding linear equation respectively, calculate spring Herba Dendrobii and become flower quality comprehensive score y1And y2Value, institute
State x1For number of sheets value, described x2For joint number value；
C. prediction spring Herba Dendrobii becomes flower quality grade：According to Equation for Calculating gained y linear in step b1And y2Value prediction, Cheng Hua
Quality comprehensive score y1＞ 1.009 and y2During ＞ 1.009, become to spend best in quality；1.009≥y1＞ -0.652 and y2＞ -0.652
Or 1.009 >=y2＞ -0.652 and y1＞ 1.009, becomes flower quality medium；y1≤ -0.652 or y2When≤- 0.652, become flower quality
Poor；
D. spring Herba Dendrobii becomes flower quality comprehensive score y1＞ -0.652 and y2The kind of ＞ -0.652 is it is contemplated that be used for cultivating life
Produce.
Wherein, leaf width 0.4 ~ 0.6 cm starts from stopping Ye Ye length 2 ~ 3 cm the leaf phase of stopping described in step a, to stopping
Uppermost leaf is gradually ripe, till Ye Ye length 4 ~ 5 of stopping cm, leaf width 1 ~ 1.5 cm.
Wherein, in described step a, stop Ye Qihou when spring Herba Dendrobii enters, from top down the 2nd leaf blade of number a length of 4 ~
During 6 cm, carry out the number of sheets, joint number measures.
Wherein, the number of sheets is the sum of plant leaf, and joint number is the stipes sum from root footpath to stem top.
Beneficial effect of the present invention：
The present invention establishes the index system of a kind of clump of plant type spring Herba Dendrobii improved seeds early screening, shortens screening varieties
The time limit, shorten to 1.5 years by 3 ~ 4 years, substantially increase the efficiency of screening varieties, reduce production cost.Meanwhile, perfect
The index system of different plant type spring Herba Dendrobii improved seeds early screening, is that other plant early screening provides theoretical basiss and side
The reference of method.
Specific embodiment
Following examples are used for the present invention is described, but are not limited to the scope of the present invention.
If no special instructions, the kind being related in embodiment is the commercially available product of Beijing Sen Hezhong industry limited company
Kind.
Embodiment 1
There are gradient disparities, there are extensively representational 6 clump plant type spring Herba Dendrobii Hybrids from becoming colored quality：Excellent product
Plant ' gloomy standing grain T-0 ' and ' gloomy standing grain T-2 ', medium kind ' firebird ' and ' gloomy standing grain 2004 ', poor kind ' gloomy standing grain H214 ' and ' happy female
2# ', blooms plant full-bloom stage respectively at the leaf phase and corresponding 3 years life of stopping of the annual seedling of spring Herba Dendrobii, carry out growth indexes and
The flower index of quality is become to measure, the data obtained is all analyzed using Excel and SPSS V18.0 software.
Growth of seedling index includes that stem is thick, section length, joint number, the number of sheets, leaf length, leaf width, plant height, fresh weight, root length, main root number,
Root is thick, its assay method：
Fresh weight（g）：The weight of the whole plant without cutting stipes, with electronic balance weighing, reading is accurate to arithmetic point
2 afterwards.
Plant height（cm）：It is the height from root neck to stem top, is measured with meter ruler, reading is accurate to 1 after arithmetic point.
Stem is thick（mm）：The thick meansigma methodss of two vertical direction stems of the thickest stipes of plant, with vernier caliper measurement, reading essence
Really to after arithmetic point 2.
Leaf is long（cm）：The leaf of top blade is long, is measured with meter ruler, and reading is accurate to 1 after arithmetic point.
Leaf width（cm）：Surveyed blade position the widest is set to the width of blade, is measured with meter ruler, and reading is accurate to arithmetic point
1 afterwards.
Root is long（cm）：When every plant radical >=3, thick the putting down of two vertical direction roots of measurement 3 representative
Average, is measured with meter ruler, and reading is accurate to 1 after arithmetic point.
Main root number（Bar）：The sum of the root directly is sprouted from root.
Root is thick（mm）：When every plant radical >=3, the length of measurement 3 representational, surveyed with slide gauge
Amount, reading is accurate to 2 after arithmetic point.
Each kind observes 30 plants respectively, takes average（It is shown in Table 1）.
Table 1 stop the leaf phase difference the annual seedling of spring Herba Dendrobii kind upgrowth situation
Note：Using Duncan multiple comparison graph, small English alphabet represents that different spring Herba Dendrobiis become the flower index of quality 0.05
Significant difference in level.Similarly hereinafter.
The flower index of quality is become to be flower thorax ratio（X1）, joint number of continuously blooming（X2）, open 3 colored joint numbers（X3）, open 2 colored joint numbers
（X4）, open 1 colored joint number（X5）, total joint number（X6）, joint number of blooming（X7）, total branch number（X8）, Flowering Branches number（X9）And always flower is measured
（X10）, its assay method：
X1：（Plant height-overhead thorax-lower sky thorax）/ plant height.
X2（Section）：The most Flowering Branches of joint number of blooming in plant, perpetual joint number.
X3（Section）：The all Flowering Branches of plant often save total joint number that 3 flowers are opened in position.
X4（Section）：The all Flowering Branches of plant often save total joint number that 2 flowers are opened in position.
X5（Section）：The all Flowering Branches of plant often save total joint number that 1 flower is opened in position.
X6（Section）：The paniculate total joint number of plant institute.
X7（Section）：The all Flowering Branches of plant often save position and open >=total joint number of 1 flower.
X8（Branch）：The total branch amount of plant.
X9（Branch）：Total Flowering Branches number of plant.
X10（Piece）：The sum that all Flowering Branches of plant are bloomed.
Wherein, plant height is the height from root neck to highest stem top；Overhead thorax is that number is all downwards from highest stem top
In Flowering Branches first Flower node to highest stem top length；Lower sky thorax is to count in all Flowering Branches the from root neck upwards
One Flower node length to root neck.Three is all measured with meter ruler, and with cm unit, reading is accurate to 1 after arithmetic point.
30 plants of the random observation respectively of each kind, takes average（It is shown in Table 2）.
The situation of blooming of the different spring Herba Dendrobii kind strain of table 2
Become flower quality quantitatively evaluating：Using SPSS18.0 software kit, each kind is become with flower index X1、X2、X3、X4、X5、X6、
X7、X8、X9And X10, carry out principal component analysiss, be extracted 3 main constituents：F1 = 0.303 ZX1+ 0.351 ZX2+ 0.366
ZX3+ 0.375 ZX4+ 0.222 ZX5+ 0.266 ZX6+ 0.388 ZX7+ 0.138 ZX8+ 0.274 ZX9+
0.382 ZX10, F2=- 0.343 ZX1- 0.308 ZX2- 0.185 ZX3+ 0.145 ZX4- 0.054 ZX5+
0.451 ZX6- 0.087 ZX7+ 0.495 ZX8+ 0.503 ZX9- 0.142 ZX10, F3=0.332 ZX1+ 0.099
ZX2+ 0.174 ZX3- 0.163 ZX4- 0.668 ZX5- 0.315 ZX6- 0.031 ZX7+ 0.515 ZX8+
0.102 ZX9+ 0.020 ZX10, its eigenvalue respectively 6.507,1.896 and 1.410, explain population variance 98.13%.With every
The ratio that eigenvalue corresponding to individual main constituent accounts for the total eigenvalue sum of extracted main constituent obtains main one-tenth as weight calculation
The aggregative model dividing：F = 0.182 ZX1+ 0.187 ZX2+ 0.232 ZX3+ 0.253 ZX4+ 0.041 ZX5+
0.218 ZX6+ 0.236 ZX7+ 0.261 ZX8+ 0.294 ZX9+ 0.229 ZX10.Wherein ZX1, ZX2... ..., ZX10
It is the value through standardization for the original variable.To substitute in aggregative model after table 2 data normalization, obtain each kind Cheng Huapin
The comprehensive score of matter is followed successively by from high to low：' gloomy standing grain T-0 '（1.905）＞ ' gloomy standing grain T-2 '（1.028）＞ ' firebird '（1.009）
The gloomy standing grain 2004 ' of ＞ '（-0.365）＞ ' gloomy standing grain H214 '（-0.652）＞ ' happy female 2# '（-2.924）.Kind grade classification is with reference to mark
Standard is：Become flower quality comprehensive score y1＞ 1.009 and y2During ＞ 1.009, become to spend best in quality；1.009≥y1＞ -0.652 and y2
＞ -0.652 or 1.009 >=y2＞ -0.652 and y1＞ 1.009, becomes flower quality medium；y1≤ -0.652 or y2When≤- 0.652,
Become flower inferior quality.
With bivariate correlation analysiss method carry out seedling stop leaf phase growth indexes and become flower quality comprehensive score phase
The analysis of closing property, result（It is shown in Table 3）.
Table 3 stop leaf phase growth indexes and become flower quality correlation coefficient
| Growth indexes | Section length | Leaf is long | Leaf width | Joint number | The number of sheets | Stem is thick |
| Correlation coefficient | .606 | -.600 | -.306 | 0.962** | 0.981** | -.190 |
| Growth indexes | Fresh weight | Plant height | Root is long | One-level radical | Root is thick | |
| Correlation coefficient | .460 | 0.882* | .324 | -.904* | -.838* |
Note：Using Pearson correlation coefficient, two-sided criterion of significance, notable in * 0.05 level；* in * 0.01 level
Significantly.
Obtain the seedling significantly correlated in 0.01 level with becoming flower quality and stop leaf phase growth indexes for the number of sheets, joint number.
Set up linear equation respectively：y1=2.061x1（The number of sheets）-8.060、y2=1.534x2（Joint number）- 7.654, r1=0.981、r2=
0.962, can effectively predict that spring Herba Dendrobii becomes flower quality comprehensive score.
Embodiment 2
Stop the leaf phase when the annual seedling of spring Herba Dendrobii enters, by when down the 2nd leaf of number is about 5cm on top, randomly select
30 plants of spring Herba Dendrobii kind ' gloomy standing grain 947 '（Better-than-average kind is evaluated as on production）, measure its number of sheets, joint number（The same embodiment of method
1）.The number of sheets is the sum of plant leaf, and joint number is the stipes sum from root footpath to stem top.
Take the average (unit is respectively piece, section) of two indices, x1=3.9、x2=5.0, substitute into linear equation y respectively1=
2.061x1（The number of sheets）-8.060、y2=1.534x2(joint number）- 7.654, obtain it and become flower quality comprehensive score y1=-0.022 ＞ -
0.652 and y2=0.016 ＞ -0.652, belongs to medium above kind, can be used for cultivation and production, consistent with producing evaluation.
The above is only the preferred embodiment of the present invention it is noted that ordinary skill people for the art
For member, on the premise of without departing from the technology of the present invention principle, some improvements and modifications can also be made, these improvements and modifications
Also should be regarded as protection scope of the present invention.
Claims (2)
1. a kind of clump plant type spring Herba Dendrobii improved seeds early screening method, comprises the following steps：
A. measure annual seedling morphology index：When spring Herba Dendrobii enters the Ye Qihou that stops, from top, down the 2nd leaf blade of number is long
During for 4 ~ 6cm, carry out the number of sheets, joint number measures, wherein, the number of sheets is the sum of plant leaf, and joint number is from root footpath Chu Zhijing top
Stipes sum；
B. calculate spring Herba Dendrobii and become flower quality comprehensive score：Randomly draw the annual seedling being in the leaf phase of stopping, measure the number of sheets and
Joint number value, takes average to substitute into corresponding linear equation y respectively1=2.061x1-8.060、y2=1.534x2- 7.654, calculate spring stone
Dry measure used in former times becomes flower quality comprehensive score y1And y2Value, described x1For number of sheets value, described x2For joint number value；
C. prediction spring Herba Dendrobii becomes flower quality grade：According to Equation for Calculating gained y linear in step b1And y2Value prediction, becomes flower quality
Comprehensive score y1＞ 1.009 and y2During ＞ 1.009, become to spend best in quality；1.009≥y1＞ -0.652 and y2＞ -0.652 or
1.009≥y2＞ -0.652 and y1＞ 1.009, becomes flower quality medium；y1≤ -0.652 or y2When≤- 0.652, become flower quality relatively
Difference；
D. spring Herba Dendrobii becomes flower quality comprehensive score y1＞ -0.652 and y2The kind of ＞ -0.652, you can consider for cultivation and production.
2. early screening method as claimed in claim 1 it is characterised in that the leaf phase of stopping described in step a from the leaf that stops
Leaf length 2 ~ 3cm, leaf width 0.4 ~ 0.6cm start, gradually ripe to the leaf that stops, and stop Ye Ye length 4 ~ 5cm, till leaf width 1 ~ 1.5cm.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101750471A (en) * | 2009-12-17 | 2010-06-23 | 华中农业大学 | Method for fast screening dendrobium nobile potted flower with high quality |
| CN103461116A (en) * | 2013-09-04 | 2013-12-25 | 北京林业大学 | Method for early screening of fine varieties of nobile-types |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101750471A (en) * | 2009-12-17 | 2010-06-23 | 华中农业大学 | Method for fast screening dendrobium nobile potted flower with high quality |
| CN103461116A (en) * | 2013-09-04 | 2013-12-25 | 北京林业大学 | Method for early screening of fine varieties of nobile-types |
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